LOCUS       NM_173598              17775 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens kinase suppressor of ras 2 (KSR2), mRNA.
ACCESSION   NM_173598
VERSION     NM_173598.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 17775)
  AUTHORS   Chen,Y., Lin,D., Shi,C., Guo,L., Liu,L., Chen,L., Li,T., Liu,Y.,
            Zheng,C., Chi,X., Meng,C. and Xue,Y.
  TITLE     MiR-3138 deteriorates the insulin resistance of HUVECs via
            KSR2/AMPK/GLUT4 signaling pathway
  JOURNAL   Cell Cycle 20 (4), 353-368 (2021)
   PUBMED   33509040
  REMARK    GeneRIF: MiR-3138 deteriorates the insulin resistance of HUVECs via
            KSR2/AMPK/GLUT4 signaling pathway.
REFERENCE   2  (bases 1 to 17775)
  AUTHORS   Popli,P., Richters,M.M., Chadchan,S.B., Kim,T.H., Tycksen,E.,
            Griffith,O., Thaker,P.H., Griffith,M. and Kommagani,R.
  TITLE     Splicing factor SF3B1 promotes endometrial cancer progression via
            regulating KSR2 RNA maturation
  JOURNAL   Cell Death Dis 11 (10), 842 (2020)
   PUBMED   33040078
  REMARK    GeneRIF: Splicing factor SF3B1 promotes endometrial cancer
            progression via regulating KSR2 RNA maturation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 17775)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   4  (bases 1 to 17775)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 17775)
  AUTHORS   Lavoie,H., Sahmi,M., Maisonneuve,P., Marullo,S.A., Thevakumaran,N.,
            Jin,T., Kurinov,I., Sicheri,F. and Therrien,M.
  TITLE     MEK drives BRAF activation through allosteric control of KSR
            proteins
  JOURNAL   Nature 554 (7693), 549-553 (2018)
   PUBMED   29433126
REFERENCE   6  (bases 1 to 17775)
  AUTHORS   Zemunik,T., Boban,M., Lauc,G., Jankovic,S., Rotim,K., Vatavuk,Z.,
            Bencic,G., Dogas,Z., Boraska,V., Torlak,V., Susac,J., Zobic,I.,
            Rudan,D., Pulanic,D., Modun,D., Mudnic,I., Gunjaca,G., Budimir,D.,
            Hayward,C., Vitart,V., Wright,A.F., Campbell,H. and Rudan,I.
  TITLE     Genome-wide association study of biochemical traits in Korcula
            Island, Croatia
  JOURNAL   Croat Med J 50 (1), 23-33 (2009)
   PUBMED   19260141
REFERENCE   7  (bases 1 to 17775)
  AUTHORS   Wang,X., Wang,T.T., White,J.H. and Studzinski,G.P.
  TITLE     Expression of human kinase suppressor of Ras 2 (hKSR-2) gene in
            HL60 leukemia cells is directly upregulated by
            1,25-dihydroxyvitamin D(3) and is required for optimal cell
            differentiation
  JOURNAL   Exp Cell Res 313 (14), 3034-3045 (2007)
   PUBMED   17599832
  REMARK    GeneRIF: These results demonstrate that kinase suppressor of Ras 2
            is a direct target of 1,25-dihydroxyvitamin D(3)in HL60 cells, and
            is required for optimal monocytic differentiation.
REFERENCE   8  (bases 1 to 17775)
  AUTHORS   Channavajhala,P.L., Rao,V.R., Spaulding,V., Lin,L.L. and Zhang,Y.G.
  TITLE     hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappaB pathways
            in inflammation
  JOURNAL   Biochem Biophys Res Commun 334 (4), 1214-1218 (2005)
   PUBMED   16039990
  REMARK    GeneRIF: We find that while hKSR-2 blocks MEKK3 activation, it has
            little to no effect on other members of the MAP3K family, including
            MEKK4, TAK1, and Ras-Raf, suggesting that its effects are
            selective.
REFERENCE   9  (bases 1 to 17775)
  AUTHORS   Channavajhala,P.L., Wu,L., Cuozzo,J.W., Hall,J.P., Liu,W., Lin,L.L.
            and Zhang,Y.
  TITLE     Identification of a novel human kinase supporter of Ras (hKSR-2)
            that functions as a negative regulator of Cot (Tpl2) signaling
  JOURNAL   J Biol Chem 278 (47), 47089-47097 (2003)
   PUBMED   12975377
  REMARK    GeneRIF: hKSR-2, a new member of the KSR family, negatively
            regulates Cot-mediated MAP kinase and NF-kappaB pathway signaling
REFERENCE   10 (bases 1 to 17775)
  AUTHORS   Manning,G., Whyte,D.B., Martinez,R., Hunter,T. and Sudarsanam,S.
  TITLE     The protein kinase complement of the human genome
  JOURNAL   Science 298 (5600), 1912-1934 (2002)
   PUBMED   12471243
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC084291.27, AC092936.9, AC079127.28 and AC073864.29.
            
            On May 17, 2019 this sequence version replaced NM_173598.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2151119
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000339824.7/ ENSP00000339952.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-915               AC084291.27        36093-37007
            916-1056            AC092936.9         195803-195943       c
            1057-1207           AC092936.9         190940-191090       c
            1208-1721           AC092936.9         96523-97036         c
            1722-1906           AC092936.9         2986-3170           c
            1907-1976           AC079127.28        72969-73038
            1977-2060           AC079127.28        76126-76209
            2061-2128           AC079127.28        96755-96822
            2129-2253           AC079127.28        100035-100159
            2254-2422           AC079127.28        115441-115609
            2423-2464           AC079127.28        123621-123662
            2465-2537           AC079127.28        124315-124387
            2538-2586           AC079127.28        128209-128257
            2587-2954           AC079127.28        130109-130476
            2955-3051           AC073864.29        33684-33780
            3052-3185           AC073864.29        34826-34959
            3186-3317           AC073864.29        42780-42911
            3318-3447           AC073864.29        48055-48184
            3448-3581           AC073864.29        49580-49713
            3582-17775          AC073864.29        52170-66363
FEATURES             Location/Qualifiers
     source          1..17775
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.22-q24.23"
     gene            1..17775
                     /gene="KSR2"
                     /note="kinase suppressor of ras 2"
                     /db_xref="GeneID:283455"
                     /db_xref="HGNC:HGNC:18610"
                     /db_xref="MIM:610737"
     exon            1..915
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     CDS             736..3588
                     /gene="KSR2"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="kinase suppressor of Ras 2"
                     /protein_id="NP_775869.4"
                     /db_xref="CCDS:CCDS61250.2"
                     /db_xref="GeneID:283455"
                     /db_xref="HGNC:HGNC:18610"
                     /db_xref="MIM:610737"
                     /translation="MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTK
                     CATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVR
                     KEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGN
                     LSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGA
                     PVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGT
                     PPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKS
                     KPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANT
                     LSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCT
                     KEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDH
                     IPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHY
                     YKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDE
                     AEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYH
                     GRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITS
                     LCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGK
                     VVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSD
                     VFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFE
                     QEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL"
     exon            916..1056
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1207
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1721
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1906
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..1976
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2060
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2128
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2253
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2422
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2464
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2537
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2586
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2954
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3051
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..3185
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3186..3317
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3447
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3581
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3582..17775
                     /gene="KSR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      17714..17719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KSR2"
                     /note="hexamer: AATAAA"
     polyA_site      17753
                     /gene="KSR2"
                     /note="major polyA site"
ORIGIN      
        1 gattgacagg caagatgtcg gtgtggagcg aggcaggagc gattgcagca accgtcaccc
       61 ccggagcccg gcggcggcag cagcagcagc agcagcagcg cggggaggag acagcagcca
      121 gccgcagtag ccgccgcagc cgcgggagca atgcagacag caccttgaga acgcgctaaa
      181 gcactccaaa ccgtggacca tgccgtgtcc ctgcaactgg gaggggggaa aaccagattt
      241 tttttgacat aaaagcaagc cagccaaaaa tatataattt tgtccttcaa ggagccggtg
      301 tgtaaagagt gagcgctatt ttccttctcc tcctcctcct ctccctcctc ctcctctccc
      361 tcctcctctc tctcctcctt ctcctcttct ttttcctcct cctcctcctc cctaagtgta
      421 cagacagcat cacatcacct ggtttgcttc tgagaaacag catctctcct tttcagggac
      481 cagagactca gagagagagg agacattcta atggagtccg cttgatatcc caaaataaaa
      541 taataaattt tggctcttgt cccctcctcc cactcccacc ccctcaaccc tgtgtgagat
      601 gttggatttc ttcttcatga gacattgttg agaagtcgca gtggttggag aggcggaggg
      661 tagactacct ctactccccc ccctcttttt tctctctggg aggaggagga ggggaagggg
      721 gttgcagagc aagcgatgga tgaggaaaac atgacgaaaa gcgaggagca gcagcctctg
      781 agtttgcaaa aagccttaca gcagtgcgaa ctggtccaaa acatgataga cttgagcatc
      841 tccaacctgg aagggcttag gaccaaatgt gctacctcca acgacctcac acaaaaagaa
      901 atccggaccc tggagagcaa gctggtgaag tacttcagcc ggcagctgtc ctgcaaaaag
      961 aaggtagcct tgcaggagcg caacgcggag ctggacggct tcccccagct acggcactgg
     1021 ttccgaatcg tcgatgtgcg caaggaggtc ctggaggaaa tctcccccgg ccagctgagc
     1081 ctggaggacc tcttggagat gacggatgaa caggtgtgcg agactgtgga gaaatacgga
     1141 gccaaccggg aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac
     1201 atgtcaggag gcaacctttc caaacaagac tggaccatcc agtggcccac gacagagacg
     1261 gggaaggaga acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc
     1321 tcccagagcc ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact
     1381 cccggggccc ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg
     1441 tgcccgcccc cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg
     1501 cacgcggtcc gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc
     1561 acgccgccca tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc
     1621 cgaaaactga tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag
     1681 ttccagctgg ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gaagagcaaa
     1741 cccttgaacc tcaagatcca cagcagcgta ggcagctgcg agaacatccc ctctcagcag
     1801 cgctccccgc tgctgtccga gcgctccctc cgctccttct ttgtgggaca cgcacctttc
     1861 ctgccttcca cccctcctgt tcacactgag gccaacttct ctgcaaacac actgtcagtg
     1921 ccacgctggt ccccgcagat ccctcgcaga gatctcggca actccatcaa gcacaggttt
     1981 tccaccaagt actggatgtc tcagacgtgc acagtctgtg ggaaagggat gctttttggc
     2041 ctcaagtgta aaaactgcaa gttaaagtgc cacaacaaat gcaccaaaga agccccaccc
     2101 tgtcatcttc tgatcatcca ccgaggagat ccagcaaggt tagtccggac agagtccgtt
     2161 ccgtgtgaca tcaacaaccc tctacggaag ccacctcgct attcagacct gcacatcagt
     2221 cagacgctcc ccaaaaccaa caaaatcaac aaggaccaca tccctgtccc ttaccagcca
     2281 gactccagca gcaacccctc ctccacgacg tcctccacgc cctcctcgcc agcacccccc
     2341 ctccctccta gtgccacgcc gccttctccc ctacaccctt ccccacagtg cacacggcag
     2401 cagaagaact tcaacctgcc agcatcccac tactacaaat acaagcagca gttcatcttc
     2461 ccagatgtgg tgccggtgcc ggagacgccg acccgggcgc cccaggtcat cctgcatccg
     2521 gtgacctcga atccaatctt ggaaggaaat ccattacttc aaattgaagt ggagccaacg
     2581 tcggagaatg aagaggtcca tgatgaggcc gaagagtcag aggatgactt cgaggagatg
     2641 aacctgtccc tcctctcggc ccggagcttc ccacgcaagg ccagccagac cagcatcttc
     2701 cttcaggagt gggacatccc ctttgagcag ctggagatcg gcgagctcat tggaaagggc
     2761 cgctttgggc aagtgtacca cggccgctgg catggcgagg tggccatccg gctgattgac
     2821 attgagaggg acaacgagga ccagctcaag gccttcaagc gggaggtgat ggcctacagg
     2881 cagacacggc atgagaacgt ggtgcttttc atgggtgcct gcatgagccc gcctcacctg
     2941 gccatcatca ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa
     3001 atcgttttgg atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc
     3061 tacctccacg ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac
     3121 aacggcaaag tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct
     3181 ggcaggcggg aggacaaact gcgcatccag aatggctggc tatgccacct ggcaccagag
     3241 atcatccgcc agctgtcccc cgacacagag gaggataagc tccccttctc caagcactct
     3301 gacgtctttg cccttggcac aatctggtat gaactccacg ccagggaatg gcctttcaag
     3361 acccaaccag cagaggcaat aatctggcaa atgggcacag gcatgaaacc caacctcagc
     3421 cagattggca tgggaaaaga aatctcggac attcttctct tctgctgggc ctttgaacaa
     3481 gaagagagac ctaccttcac caagctcatg gacatgctgg agaaactgcc aaagcgaaac
     3541 cgtcgcctgt ctcaccctgg acatttctgg aagtctgcag agctgtgacc tttggacatc
     3601 gggacggcgc ccagctgcct gggctcccgt cacctctccc tccctactct gtcctctgcc
     3661 agctcaggag gccagagact cagcatcaga gggtaccaac cccgaccgac ctgggagcac
     3721 tggacaactg ctcggcggcc tccctgcacc tcagacttga gcccttttcc tcgggcaggg
     3781 ttggggaccc ccgagctagg actgaaccgg accagccagg gtgatgcgtt attgatcagt
     3841 gcggcccagg ggacagcact aatgagagca ctgcaggctg cacgtggtgc ctttgggcta
     3901 tgggactagg acaggagacc ccacgtgggg gggctcacag acggggctat gtgtgtgtgt
     3961 gtctgtgtgt ttgacgttgt tcactcttgt ttccccttga caagcaagag ccagactgtc
     4021 ccaatgacgg ggcactcctc agttctgtct gtatgtggta ggttccagct gtaccatctg
     4081 ggaagctaag aggagggtac agagccgagt ggcagcttag tcaaggctac tgagccaaga
     4141 tcgagcaggg aaggtcggga gtggctaagc tcgttccttt ggaaatatct gttttcagac
     4201 accccaaccc gagcctgtca tggcgtgtgg cacctttgca tagagctctg aagtacagta
     4261 ggaaagttag aacgtctttt gagttctggg tctctctggg ggacctattc ccccatttct
     4321 gctgttttct cctgttctct gagagtagca tcccttttca cctcactgcc cgcacctcct
     4381 tgccatttgg aaagccaaga tgacttctga ggggctcctt agagctcacc gtcattcagg
     4441 gcaccccctg aagtctggtc cctgagaatc agggctctgg gcagggagtt ctaggacatt
     4501 ccttttcttc cctcttctct gctactcaca ccagtgaagc ttattccccc accaaggtgg
     4561 atgcgggttc ttggggtacg tccatgttgg gttatgtggc tgtacctatg ataacacgtt
     4621 tgcaaaagat ttccctgcct gtcacccttg aggagtcctg ttgggggagc acaaagggag
     4681 gatgtttgtt acacttctgc gttctttgct ctagcctggt gcggggtcct caagtagctt
     4741 ccccatcgga agcctgtgtc cctcacctgt gtgtgttccc tcagtgcccc accttccctc
     4801 cctcctcttg atcccaacct gggccggcga ctcttcctga ccatgttggg gaagcccctg
     4861 gtgttctgta tctgtaggaa ggatgccgag agcaggctgg gcatgcagat gggagaggag
     4921 aacctctggg tcatttgccc atgttttcaa agccctgacc ccctgcatgt gacctgatac
     4981 aaaagggaaa tctgtcccca agcagagcaa tagcaggatt atctgggcag tgaacaggaa
     5041 gcccagggcg gccacagccc ctctgtgttt cctgggtact acctgggcct tagttttctt
     5101 gtagctacta tctatccagg catctccctc ctagagggcc agagagagga actggaactt
     5161 caaaaaccct acctgatctt actttcaaaa tccaaataga cctatgagga tctcttcccc
     5221 accttgggtg actcccagac tggttcatgc tctgggggcc aaacctgctt ccaaaccggg
     5281 aagaatggtg agatcagtca tgcttcccag gagcctggct acaggaaacc cctgtgtttc
     5341 ccaaagggcc ctcctgaatt gcctttccaa cactcccagc ttactgggac tagaggacta
     5401 aagaccccgg cttgggctgc tcctaagcag acactgctct tctggggaca tctatggctc
     5461 ttggcttgac gacgtgtgtg tgtcttggag gcattttata tatgtttgcc tgagccccag
     5521 ccactctggg gttgctgggg atggctcttg tcaatggaca cctccccatc ccaacacaac
     5581 actctgtgtc ccagccctgg gcctggactt gcacctgtct tggctctgcc cagcagccta
     5641 ctgagtgcct gaaacccaac cactcacttc tcttccatca caccaggact tcttgggtcc
     5701 acaggctcca gtccttgtca tgttcccttg tgccaagaga caggatatga ggaagggaag
     5761 aggcagccag ggtctactac cctgcagctg aaatgtccac aagaactggc accttctcac
     5821 tcttgcctct cttgtttcca gaattaacta aaatgtccaa ccacagcctt tggcaaggcc
     5881 tagcatgatc tccaacagct gatttttcca ttcattcatg agcaccagtt tgggattaag
     5941 gacagggcct gagtgatcca ggggaaaacc cagccctttg tggaacaggg tgccttgggt
     6001 ccatccctga atctgagtca cttgctcttg acttctcgat ctttccccac ttcactactc
     6061 tgtccccacc tgcattggtc tctgctgcag ccaggccagg atcctggcct tcagtgcctt
     6121 cctgacgccc ctaccctgtc ccctctacac cgccctgact tgttgagaaa ccagctttaa
     6181 tttgcacaca tttaaatcca ggaagaacca aaccgctgct ttccctgctg gcttccatct
     6241 ttattcgctg atgaggtttt gggaggaggt tatattcatc ttacgcaggg aggaaaaaaa
     6301 aagaggaaag aggctgtata tttttacatc ataattacca gcctcacctg caactcattc
     6361 tatacaatga cacgaccaag atttgtgtgt ttgcccttgt cccatcacag ctgcacacag
     6421 gtgccctgta cacataagtc actgactcag cagcgagttg ttccagtcag tcttgctttt
     6481 agcaaaccgt catatacatg ggcttcccga ggggaccagt ccacagggtg aagattcacc
     6541 aggaagcctt ggctttgcag agagaaacat ctcagctttc tttaaagggc atgtggattt
     6601 gcacataaat ttcacccaac tcttccagga tacagattat tcataaaacc aaggagcttc
     6661 cttcttggcc ccctgtggga ggacgtgccc cttgtgtccc catccccaga gccaagctgg
     6721 aaacccaaag tcattgctta gatccatttt gtcctggtat tccttttact gatcaaagag
     6781 aaaattttca tcttgccatg gaccccccca tcctcacccc atccacttgt ctccacagct
     6841 accttggatt gtcagtcaat agaagccctt gactgatatt gaccactaga cttttgtatc
     6901 cagggaacct agttcttccc tttttctcac ctcccatgcc atatacacac atcatgcccc
     6961 tccccacaaa cacacgaaac cagccttatc tcaccttcca ccattcccat ccccacctac
     7021 agctgatcat caaaggtaca cggaggaagt agtagacacg gagctcaggc caatcattat
     7081 cttgagaaat ctaaacccag ggctgtcagg ctatgcccca taggccatac ccagcctgcc
     7141 aaatggtttt tacatgtttt aattgaggaa aaatcaaaag aataatattt catgacatga
     7201 aaattctata acattcaaat ttcatgtcaa taaacaaaat gttattggag cacagtcacc
     7261 tctccattta cacaaccacg gctgctccta tgcaactgtg acagagccag ctatttgcaa
     7321 cagagacgat gtggcccaca cagtcaaaaa cttttacaat ctggcccttt cctaactttg
     7381 ctagcctttg atagagagga ttggatatgg ctgtcagggg aggaacagga gcctttgata
     7441 gagagggttg gatatggctc tcaggggagg aacaggaagt tggaggggga gagaacttgt
     7501 ttcagcggaa gaaggccagc ttgccccaag atgcagcggg ctgcctccgg aagtagagaa
     7561 ctccctgtcc ttggataggg tggagcagat gttgactgct tggcctgtgt gatatggagg
     7621 caattcaggc cctgaaggct ggaccaactc cctggacttt aaaactcttc gtttgctaag
     7681 gggggccata ataacaaaat gtcacacact gagtggctta aacaacacga atttattcct
     7741 cacaattctt gaggctggaa gtcccaagat caaggtgtca gcagggttgg tttctggtga
     7801 ggcctctttc tttggcttgc agacagctgc cctctgatcg tgacctcacg tggcctttcc
     7861 tctgtgactg cacatcccca gtatctcctc ctcttctgat aaagacaaca atcttattag
     7921 attagggttc caacgctctg tgattttatt cccatccccg gctttctttc cccacactgg
     7981 ttctcaatct tgagtcccga aatatgccct ccctatcctc atcctgtgcc taccttcact
     8041 tcacccatcc atcccctgca ggctggtctt gttccactgt gccaccatat ccccaagccc
     8101 ccctcacctc cgtgaggctc ctaggactgc tgcagcaaac caccacccac tggggcctac
     8161 aacaacagat gtatcatctc tcacccatct ggatgccaga agtccaaaat caaagtgtta
     8221 gcagggccat gttccccctg aaatctgtaa gggaatcctt cctggcggct tctgaattcc
     8281 tggtgctttc ctggtcatca ttggcattcc ttggcttgca gctgtgtcag tccaatccct
     8341 gccctcatca tctctctgtg tgtccctatc ttcatatggc catcttctta taaggacacg
     8401 agtcacattg gattaagggc ccaccctatt tcagtatgac ctcatcttaa ctaattatat
     8461 ctgcaaagac cctctctcca aatgaagtct cattctgagg tactaggaat gaggacttca
     8521 acatgtcttt tttttttttt tttttttttt gttggggaca ccacaactta taacaccctc
     8581 taagacattt tctcttgtgc cttgactggt cctccactgt agaacccagg tgcccttgaa
     8641 gatagattcc ccagaattcc tggtctacaa ggtcaggctg ttgctgtcat gcttaccaag
     8701 cttgccccaa tcaacacgtc ccatcactga attccaacag ctctggatct cagttctgtg
     8761 gctgtatggg aacagcctgt gtgtaagtgg cttgtctgta gcggactgtc acttgacccc
     8821 tgaatgtgcc tcatgaccct atccaaagca gttggtgaca cagcatctga ttttatctat
     8881 actcttgttc attgacaaag gagtggattt tgatgagcta cattttgctg tttccttttt
     8941 tggctttgcc tgtgatgtgg ggaagttcct aggcagactc atgtggccac tctccctggc
     9001 tcccagcctc acctcctccc gtaccacctc accctcacct gcacacacct gcaagggaat
     9061 cactttgaac tccaagggga gtgacaggca gcaaatgtca gtctgggtcc tggaatgtgg
     9121 agccgcagtg aacctctgtc ctctctggtc agtgcctgac tatgatatta acccaaatcc
     9181 cagactccct tctcgaggcc ctgcaatcca tacccattct gttaccagtt tagttttgaa
     9241 accagtagtg gggcaggagg ttgatggaaa atctgcggtc ccagtgacct cctggacttg
     9301 tcttagtagt gcctggcaca tagtaagtgc tcaagaaatg tttacagaat gcagacagag
     9361 aggtttgctg ggtaagagta agaccctggc acagggagtt agaagggcac cattggcagg
     9421 gctggatggt attgggattc ccctttgcca gaccatgttg tgggtgccat atcaacccca
     9481 atgagtttgt ttgttaggtt tgttgttgtg gtttagagtt agagtcttgc tctgtcaccc
     9541 aggctggagt acagcgtgat catagctcac tgcagcctcg aactcctggc cacaagcaat
     9601 cctcccacct tagcctctca agttgctggt actacaggtg cacaccatca tgcctggcta
     9661 attttttatt tgttgtacag acagggtctt gctatgttgc ccgcgctggt cttgaactcc
     9721 tggtctcaag caatccccct gcttcagcct cccaaagcac tgggattaca gggctaagcc
     9781 attgtgctga gccagtttgc caggtttaat atctgtgacg tatgaagcga cccattgagt
     9841 caggtggccc aggatgtcta ggaggcagag taacaatcca ccctgaacag ccctcattca
     9901 cagagcactt actacgtgcc aggctggaat tcctatgatg gtttggggag atcagtagtc
     9961 ttattctcgt tccaatgggg caactgtggt acagggacag aaaccagcct ccaaattgca
    10021 gacccagcat tttaaaaacg agtctttggg ctcatccatt cccaggaacc cacctccagg
    10081 tcaaggggtt catgtccagc tgctcatggg gatgttacaa tcccagcctc tgagacttat
    10141 tggcaacttg gaaccaggaa agagagagag gaaaagcagg aagttgtgga agggagggtg
    10201 caggggctgg cagggaccat actgggagtt tgcacggaag cttgaactta tgttctcaaa
    10261 ctgacaggct gccagcagca gcccggattt ccaggaaatg tacatctggg ttggcagagt
    10321 ccctcctggg tccctccagt atccctgctc caggcttcct atcccctccg tctctcctgc
    10381 tggatgttgc actgtggtcc cgtgttcctg agaggcgtac atcagtgagt gctcttccct
    10441 ctcaggggtg ggaaagggcc cttccttccc actcattggg atcatgcagc tcccatttcc
    10501 taacaccaag aatatgaagg attcatctca tggggttgag aatggctgtc ctccttccac
    10561 tgaggactga gctggaggag atggactctg tctagaatgt gcctgatcta aataagacct
    10621 tgtttcccaa gcatgtccca tagacctttc attccatgga aaactcgccg gatttgcatt
    10681 attgaaaggg tcctgggaca agcatgcatt ggaaatgctt aggtaaaatg aaactaattt
    10741 cttgattgca ggacttatca gaacttttaa gacactattg tgtggttgaa tctctaaatg
    10801 cctgatagga ggctgttctt cccaagcttt cccaggcttg tttctgaatc tccaacaagg
    10861 tcaacctctg tgaatgagac ttgatacctt acctggtact gaaggaggca atgtcaatgt
    10921 actggcccac agaaaagagc tgaccactag cattcataga cactgctagg agatgagaaa
    10981 ggtagcttag agcccgattc catccatgtg gacagtggtt ccatgaacaa gaacaccaca
    11041 tctggctgat ggtgtcttcc aatggagggg tcaaatctgc tgtgtgttgg tggcttctcc
    11101 aacataccat ggcttggagg ccaatgtgaa aagtatataa gatgtaacac cctcagaagg
    11161 gttaagtttt cacctgagtt ttcatcttca accaagtcat tgggatggtg atctagaagg
    11221 acctctaaga tctagaagga taaagatctg gtccaaggag tacaaatatg taatggatct
    11281 accaccagtg tcccctgctg tactcttggc agacatcact aatccatcac agcatgcctt
    11341 cccactgatc acagatgtag cttcagggca ttcgagttac cacttaagat ggatatgagt
    11401 gaccctccct tatctcactc ttctcctttc acagaagcag gatctgaggc ccagagaggg
    11461 aagctataat aaactcccct aagtcaggtc ccttctaaga ggcagaggta ggtttagaac
    11521 tcaggcctca gaaacttcca gaagccaagc catttgggct gtagttggtg gacagtctat
    11581 cagcgctctg cagatgtttc ttctcagtta gggaacttgg aaaatgcaga aagaaagatt
    11641 ctgtctctaa gactctgtgt atgatcctac tcggtaatct gcctgcttgg agaaaaatca
    11701 tttatcttct gggacggctt caccacaagg cctgaatgct atgcccagtc tagtaacagc
    11761 tgggctggaa gtcacggcat atgggggcct taaggaataa cagtgtgcgt ggtggtgtgc
    11821 aggagagctg caagggcttt tctttcttga ggaggtttgt aaaatttctg ctgttcagtc
    11881 tgggcttctg ggaagtcagg gggtgctgca tataaagggc aggaaaagga gcctgactcc
    11941 aattgctctt tggtttcttc cctgaatgga aggctcaaaa tcaagtttcc acccagataa
    12001 gatcttgagg tcctagtgga ccaccagctc tactcctgag ctcttgaaat acctctaatc
    12061 accagctcgg tggctgagag taccttcttg tccactgcct cttcttttcc ttctcgtctt
    12121 cacttatccc ccatccaact tctgcccgat aatatctccc ctgtattgta aactgaggag
    12181 aaagttgcac aattgtagag atgaaaaata gctgccatgt atttagctta aattagctca
    12241 gaagaaaggc tccctccctc cattgagcgg gcagcacttg tttatgccaa catgctattt
    12301 gcatcgcgcc ttcttaaatt tgaaagtcta atgattaaaa acaaatctta gtttttcttt
    12361 tccaccactt aaaaaaaaaa aaccagcttg gctagttctt ctagtttaaa aataccctga
    12421 cctccagcac tacagtgaga attttggaaa ggctggtacc aggatatcag gaggaaataa
    12481 atacaggcca acaataacca gaggcctttt ctgagtggtg ggcgacagga tgaacaaggt
    12541 agaagagagg tctttgtgtc ctcacacaga tgctggcatt ctgggtttgt ctggaattca
    12601 gtgaacctct gtcctctctg acagagattt cttcaccaac cattcattca gtgggtgatt
    12661 tgttgagcat ctactatgtg ttagaccctg ggcattcagt gatgagtgat acaatgtctc
    12721 acctgttctg aagcctaaag cccaatgggg aagagagaca agaagcaagt caaccacaaa
    12781 tatataaatt tgcaactcat gtagatctat ggaaagaaaa ctcacggggg accagaaaca
    12841 tgggagaggg tatactgtgg gagagctgct gacaaaggtc tttctgagga ggtgacattt
    12901 aagtcaagat ctaaaggaag agaaggataa agagatgttc attctaagcc agggtttctc
    12961 aacctcagta ctgttgagct atttggggcc agataattct ttgaggtggc gactgtcctg
    13021 ggcattggag gatgttaagc agcttcctca actctcccca caagatacca gtagcacctg
    13081 gtactggtga catccaaaaa catccccaga cacaccctga ctttgctgtc tggaatcaat
    13141 cctcataaag ccccaagcct tggagcacag aggctggaag cccatatggt ggcatgtcag
    13201 gcatggtgtc acctgagctt ctctgacccc cacttctgat atggcatcca gtttggagtc
    13261 tctgttctcc atgagctccc atagctgtcc catattccac aaggcattgc ttctgggcca
    13321 cggtcctcta gttccagcat agccctattt ttgggtatgt tggtggattt caaaagcctc
    13381 cagttgacat ccaaagtgtg acatccaaaa ccatctccag acattgccag atgtcccctg
    13441 agttgtctcc aggccacgcg actaggattc aaatccagca tcctgccatc tgcaaccttc
    13501 catgggagaa aggaaatcag aggagaacag gaaaacacaa ggaggggact ccttaagtga
    13561 acattgcagg gaaataggtt tttaaagcac tagttagcat caccagtggt ttgaaggctg
    13621 tctaggtaag gagaatgtgg acttgccttt caccgtctgc acggacaggg gctgaaatct
    13681 ccagtcattc tctccatcct tccacagccc ttctaagaag cagatcctct tgctgagctg
    13741 aaatcttgag acttgcatcc ggcaatcttg gtgttgcttt ctgaggctac tcagggaaag
    13801 cccttctgta ttccccagga aggaaagtct cccagatagt gaagggcctt ttctaccttc
    13861 cttgcaattc ccagttcact gtgaagaaga gagtcaggcc gggttagaat ggctgtctct
    13921 ggtagcaatg aagcaacaac atagacagtc tggacaagat gtggcagttc tgtccagtcc
    13981 cgtgtgactg atttgggcat ggagacaggc agtgggtgag gatggcctct gtaggtccag
    14041 ctgttttacc cctagaatca aatccctgga gcttccggca gtcccaggat tgggaagggt
    14101 aacagagagg acgagtcggg cactgcaaaa gaccagatct tggaaaattg ctttgggaat
    14161 tgggacacct tccaagaaga cagttgagtt ggctggggct ctgccctaga tggggtcaac
    14221 ctctccccaa cacagccctg cccaggttgg ggcattggca gcttaagtgc gtcctggaca
    14281 acaaatgtaa tgtattggtc ctgtaagatt ctagccattt tcctctgctt atttttctac
    14341 atgtgttaca agtaataatg tttagatggg tacatatgaa cttgtgtaag ttcaagtaac
    14401 tgctatgtag ctacagcatg ggcatcaagg tactctaagg tacttgccct gccacaccga
    14461 ggccccctgc cccggccccc tttccctcct agccagcctt ctagggagca ctgcacaccc
    14521 tgccccttca aagctatctg gacagtcctg gctcgactgg agcattaagg gaggggctgt
    14581 ccttggtcag gcttctgccc aactgtgtga tgcataaatc tttgcctggc tccttcttcc
    14641 ttcttttcgg aagtctatct cctcctgagg gcctctgcgg tcctaaccta tcaaggaata
    14701 tcatagattg tactccccac cccatcaaaa cacaccctga ctttgcgtct ggaatcaatc
    14761 ctcataaagc cccaaggctt ggagcacaga ggccagaagc ccatatggtg gcatgtcagg
    14821 catgctgtca catgagcttc tctgactcca cttccaagat ggcatccaat ttggagtctc
    14881 tgttctccat gagctcccat agctgtccca tattccacaa ggcactgctt ctgggccccg
    14941 gtcctctagt ttcctcatag ccctattttt gggtacattg gtggatttca aatgcctaca
    15001 gttgagcagc cgaaggattc taagaaaatt ttgcttgtat ttcaaaggct catgtcccca
    15061 gccccattca ctacaccctc agcagagccc agcgtggagc aaccttgtcc cttcattccc
    15121 caaatcttca ttttatatag tcatttagta aatatttgtt aagtgcctcc tgtgattctg
    15181 gaaaaaatgg agaaaagtgg gtgctgcctt cagggtacta ggagtccgat gaagagaggg
    15241 aggagttcca gtagggtccc gaggccccac aggagctccc aacccagaga aagaccctcc
    15301 aggaaggatt ctcagaggag gagacttctg aaccctgtct tgaaatttga gtagaatggt
    15361 agggaacggt gtgtcagaca gaaggaacag cctatgtgtg gactcagagg caagatcatg
    15421 atttattgag ggtaactgca agtggctaaa atcctgcccc ctggcacttg cctgcctttc
    15481 cctgctttcc ccaaagcctg attttgggac cctggagtct ccatctccaa acagagggtg
    15541 ccctggctca gatgtggaga ccttgacttc tttccccagc atgctctggt tcctactgtt
    15601 agccttggtg tcctgtgggg gaagatgtct gaggagagtt ttgtgcttgc tggagtcaga
    15661 ccagtactca ggccacctca ggcctgctct gcatccctct ctggctctac agacctctct
    15721 ctctctctct ctctctctct ctctctctct ctgtctgtct gtctctgtgt ctgtctctgt
    15781 ctctgcctgc ctccacacag tgaaggatgt gtctctattt cttagtgccc tgcattgggt
    15841 cctgaagtcc atgggcatct tggctataga tgggttggcc atgagccctt ctctctaatg
    15901 catttttcat tcctgttcct cttttgcttc actttccctc tatcgactct tgcgacctgt
    15961 gtctctgatg tctctggggc ccccagttct gtcttgccat accaaaaaac cttcttcagc
    16021 aaaaaacctc tttctgggac agttcatatc actctatgaa taggcccctg aagtgttgag
    16081 gtcaaggaca tgtgtgaacg tggaaatgta tttatggaaa gaccctacac tgagggtctc
    16141 taactaaaca tttcaggtat gatgggcaaa atgctggttt gttacagaaa tgactgaaga
    16201 agacctaggg gccatagtgg gccacaggcc aagcctgcaa tacagcctga agtgtgggaa
    16261 agaatgtatc agtcctgttg atggagtggt gtgtttgctg tctctataaa tatgtcaggg
    16321 tttgaaagac agaaaacaaa gcatctttgc tgaccgccag actcccaggg tatatgtggc
    16381 tactgcaaat tagcgaggct tgtttatctg cttcaacttg actcaatttg catttcttga
    16441 gccactgtct attgtggata atgatttcat caacactttc tcagcatcag cagcccctcc
    16501 agagtattcc ttgaattttc ttagctttgt gcaacccacg agcacttaat atatcactaa
    16561 cccttgagtt tattggaggg agtgaatgaa ataatgaatg tcaataacgt tacgcaatac
    16621 ctggcacaca gtaggtgctt catcaatgtg gcttttcttc tcaatcaccc aaatgaacct
    16681 cccccagaat cccacctcta gggggacatt cctgaaaaat aagggactag aatagcgagg
    16741 tggtgggtgt tacccattta tttagatatt tgtgtcctgg tgaacatggg gagggatgcc
    16801 caagtgtcat aaggacaccc cctccccccg tctacaaggc agaggtctct taggatgtga
    16861 gagagtctct ggcaagacca gcccccaccc caccaaggcc ctcatagaaa cagaacattc
    16921 ttcctcccta tcccaaagca gaagttgact ggggcagata aggcaagtca cattacatct
    16981 gtgcccttga ccatacctgg accaggcaga gaagatccca gacttaggtg gtcaacgttt
    17041 ccaccccaag cctctcattt ctcgctctct ttgagacttg atcttgtacc aggatccact
    17101 cgacagactt aacgcatgat taatttgatt gattactctt gaagttactg accttaggaa
    17161 aattcctcaa ttgtaaaaag gctttctcta tatgtaaatt atgctgttat ctttttctat
    17221 acacaaagta tatagaccta taaatattat acatatatat tatatatata aagactaatt
    17281 cttgtacaga ccaacgcgga ccgatggtcc atattctttt ctccatgttt gtttgtgtcg
    17341 actgttgcag agtgagttga tgtaagaagg tggtttcatg ttttcttttg gggggccact
    17401 tctttctttt cctttccgtt tctatctctg tctgtctccg tctctgtctc tctctttctc
    17461 acttgttgcc aatgtcttgt tgctgagaag gaacactcta ttcagatgcc atccaggaaa
    17521 gaaaggccag atggatggag ggtccactgt gtatatttgt acagaatcat ttataaagtt
    17581 ttctacactt ctaaaaaaag tatttctaac ttgggctgtt ggacagtttg tgtgcctctg
    17641 agccagcaag cctgttgttt cttgtgttta gtgcaatgtt tagtgagaaa ccaatgtctg
    17701 aattatttat gccaataaaa aatttgagaa ttactgcatt gcactactgc caagacaggt
    17761 ctgaattcta ttgtt
//
