LOCUS       NM_002273               1784 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens keratin 8 (KRT8), transcript variant 2, mRNA.
ACCESSION   NM_002273
VERSION     NM_002273.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Li,K.J., Hong,Y., Yu,Y.Z., Xie,Z., Lv,D.J., Wang,C., Xie,T.,
            Chen,H., Chen,Z.S., Zeng,J. and Zhao,S.C.
  TITLE     NAT10 Promotes Prostate Cancer Growth and Metastasis by Acetylating
            mRNAs of HMGA1 and KRT8
  JOURNAL   Adv Sci (Weinh) 11 (32), e2310131 (2024)
   PUBMED   38922788
  REMARK    GeneRIF: NAT10 Promotes Prostate Cancer Growth and Metastasis by
            Acetylating mRNAs of HMGA1 and KRT8.
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Kuzmuk,V., Pranke,I., Rollason,R., Butler,M., Ding,W.Y.,
            Beesley,M., Waters,A.M., Coward,R.J., Sessions,R., Tuffin,J.,
            Foster,R.R., Mollet,G., Antignac,C., Edelman,A., Welsh,G.I. and
            Saleem,M.A.
  TITLE     A small molecule chaperone rescues keratin-8 mediated trafficking
            of misfolded podocin to correct genetic Nephrotic Syndrome
  JOURNAL   Kidney Int 105 (4), 744-758 (2024)
   PUBMED   37995908
  REMARK    GeneRIF: A small molecule chaperone rescues keratin-8 mediated
            trafficking of misfolded podocin to correct genetic Nephrotic
            Syndrome.
REFERENCE   3  (bases 1 to 1784)
  AUTHORS   Pranke,I.M., Chevalier,B., Premchandar,A., Baatallah,N.,
            Tomaszewski,K.F., Bitam,S., Tondelier,D., Golec,A., Stolk,J.,
            Lukacs,G.L., Hiemstra,P.S., Dadlez,M., Lomas,D.A., Irving,J.A.,
            Delaunay-Moisan,A., van Anken,E., Hinzpeter,A., Sermet-Gaudelus,I.
            and Edelman,A.
  TITLE     Keratin 8 is a scaffolding and regulatory protein of ERAD complexes
  JOURNAL   Cell Mol Life Sci 79 (9), 503 (2022)
   PUBMED   36045259
  REMARK    GeneRIF: Keratin 8 is a scaffolding and regulatory protein of ERAD
            complexes.
REFERENCE   4  (bases 1 to 1784)
  AUTHORS   Chevalier,B., Baatallah,N., Najm,M., Castanier,S., Jung,V.,
            Pranke,I., Golec,A., Stoven,V., Marullo,S., Antigny,F.,
            Guerrera,I.C., Sermet-Gaudelus,I., Edelman,A. and Hinzpeter,A.
  TITLE     Differential CFTR-Interactome Proximity Labeling Procedures
            Identify Enrichment in Multiple SLC Transporters
  JOURNAL   Int J Mol Sci 23 (16), 8937 (2022)
   PUBMED   36012204
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1784)
  AUTHORS   Xiong,F., Guo,T., Wang,X., Wu,G., Liu,W., Wang,Q., Wang,B. and
            Chen,Y.
  TITLE     Keratin 8 Is an Inflammation-Induced and Prognosis-Related Marker
            for Pancreatic Adenocarcinoma
  JOURNAL   Dis Markers 2022, 8159537 (2022)
   PUBMED   35958278
  REMARK    GeneRIF: Keratin 8 Is an Inflammation-Induced and Prognosis-Related
            Marker for Pancreatic Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1784)
  AUTHORS   Ljubimov,A.V., Bartek,J., Couchman,J.R., Kapuller,L.L.,
            Veselov,V.V., Kovarik,J., Perevoshchikov,A.G. and Krutovskikh,V.A.
  TITLE     Distribution of individual components of basement membrane in human
            colon polyps and adenocarcinomas as revealed by monoclonal
            antibodies
  JOURNAL   Int J Cancer 50 (4), 562-566 (1992)
   PUBMED   1371500
REFERENCE   7  (bases 1 to 1784)
  AUTHORS   Waseem,A., Alexander,C.M., Steel,J.B. and Lane,E.B.
  TITLE     Embryonic simple epithelial keratins 8 and 18: chromosomal location
            emphasizes difference from other keratin pairs
  JOURNAL   New Biol 2 (5), 464-478 (1990)
   PUBMED   1705144
REFERENCE   8  (bases 1 to 1784)
  AUTHORS   Yamamoto,R., Kao,L.C., McKnight,C.E. and Strauss,J.F. 3rd.
  TITLE     Cloning and sequence of cDNA for human placental cytokeratin 8.
            Regulation of the mRNA in trophoblastic cells by cAMP
  JOURNAL   Mol Endocrinol 4 (3), 370-374 (1990)
   PUBMED   1692965
REFERENCE   9  (bases 1 to 1784)
  AUTHORS   Smedts,F., Ramaekers,F., Robben,H., Pruszczynski,M., van Muijen,G.,
            Lane,B., Leigh,I. and Vooijs,P.
  TITLE     Changing patterns of keratin expression during progression of
            cervical intraepithelial neoplasia
  JOURNAL   Am J Pathol 136 (3), 657-668 (1990)
   PUBMED   1690513
REFERENCE   10 (bases 1 to 1784)
  AUTHORS   Krauss,S. and Franke,W.W.
  TITLE     Organization and sequence of the human gene encoding cytokeratin 8
  JOURNAL   Gene 86 (2), 241-249 (1990)
   PUBMED   1691124
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA825311.1, BC075839.1 and
            BE300986.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_002273.3.
            
            Summary: This gene is a member of the type II keratin family
            clustered on the long arm of chromosome 12. Type I and type II
            keratins heteropolymerize to form intermediate-sized filaments in
            the cytoplasm of epithelial cells. The product of this gene
            typically dimerizes with keratin 18 to form an intermediate
            filament in simple single-layered epithelial cells. This protein
            plays a role in maintaining cellular structural integrity and also
            functions in signal transduction and cellular differentiation.
            Mutations in this gene cause cryptogenic cirrhosis. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation in a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and
            3 encode the same isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063513.1, SRR7346977.396177.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000692008.1/ ENSP00000509398.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                DA825311.1         10-56
            48-1750             BC075839.1         1-1703
            1751-1784           BE300986.1         11-44               c
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..1784
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /note="keratin 8"
                     /db_xref="GeneID:3856"
                     /db_xref="HGNC:HGNC:6446"
                     /db_xref="MIM:148060"
     exon            1..419
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1547
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /note="isoform 2 is encoded by transcript variant 2;
                     keratin, type II cytoskeletal 8; cytokeratin-8; type-II
                     keratin Kb8; keratin 8, type II"
                     /codon_start=1
                     /product="keratin, type II cytoskeletal 8 isoform 2"
                     /protein_id="NP_002264.1"
                     /db_xref="CCDS:CCDS8841.1"
                     /db_xref="GeneID:3856"
                     /db_xref="HGNC:HGNC:6446"
                     /db_xref="MIM:148060"
                     /translation="MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSS
                     NFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKF
                     ASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLK
                     LEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLT
                     DEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRA
                     EAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAA
                     IADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYR
                     KLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS
                     SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK"
     exon            420..628
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            629..689
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            690..785
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            786..1076
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1297
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1356
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1784
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1759..1764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /note="hexamer: AATAAA"
     polyA_site      1784
                     /gene="KRT8"
                     /gene_synonym="CARD2; CK-8; CK8; CYK8; K2C8; K8; KO"
                     /note="major polyA site"
ORIGIN      
        1 attcctgaga gctctcctca ccaagaagca gcttctccgc tccttctagg atctccgcct
       61 ggttcggccc gcctgcctcc actcctgcct ctaccatgtc catcagggtg acccagaagt
      121 cctacaaggt gtccacctct ggcccccggg ccttcagcag ccgctcctac acgagtgggc
      181 ccggttcccg catcagctcc tcgagcttct cccgagtggg cagcagcaac tttcgcggtg
      241 gcctgggcgg cggctatggt ggggccagcg gcatgggagg catcaccgca gttacggtca
      301 accagagcct gctgagcccc cttgtcctgg aggtggaccc caacatccag gccgtgcgca
      361 cccaggagaa ggagcagatc aagaccctca acaacaagtt tgcctccttc atagacaagg
      421 tacggttcct ggagcagcag aacaagatgc tggagaccaa gtggagcctc ctgcagcagc
      481 agaagacggc tcgaagcaac atggacaaca tgttcgagag ctacatcaac aaccttaggc
      541 ggcagctgga gactctgggc caggagaagc tgaagctgga ggcggagctt ggcaacatgc
      601 aggggctggt ggaggacttc aagaacaagt atgaggatga gatcaataag cgtacagaga
      661 tggagaacga atttgtcctc atcaagaagg atgtggatga agcttacatg aacaaggtag
      721 agctggagtc tcgcctggaa gggctgaccg acgagatcaa cttcctcagg cagctatatg
      781 aagaggagat ccgggagctg cagtcccaga tctcggacac atctgtggtg ctgtccatgg
      841 acaacagccg ctccctggac atggacagca tcattgctga ggtcaaggca cagtacgagg
      901 atattgccaa ccgcagccgg gctgaggctg agagcatgta ccagatcaag tatgaggagc
      961 tgcagagcct ggctgggaag cacggggatg acctgcggcg cacaaagact gagatctctg
     1021 agatgaaccg gaacatcagc cggctccagg ctgagattga gggcctcaaa ggccagaggg
     1081 cttccctgga ggccgccatt gcagatgccg agcagcgtgg agagctggcc attaaggatg
     1141 ccaacgccaa gttgtccgag ctggaggccg ccctgcagcg ggccaagcag gacatggcgc
     1201 ggcagctgcg tgagtaccag gagctgatga acgtcaagct ggccctggac atcgagatcg
     1261 ccacctacag gaagctgctg gagggcgagg agagccggct ggagtctggg atgcagaaca
     1321 tgagtattca tacgaagacc accagcggct atgcaggtgg tctgagctcg gcctatgggg
     1381 gcctcacaag ccccggcctc agctacagcc tgggctccag ctttggctct ggcgcgggct
     1441 ccagctcctt cagccgcacc agctcctcca gggccgtggt tgtgaagaag atcgagacac
     1501 gtgatgggaa gctggtgtct gagtcctctg acgtcctgcc caagtgaaca gctgcggcag
     1561 cccctcccag cctacccctc ctgcgctgcc ccagagcctg ggaaggaggc cgctatgcag
     1621 ggtagcactg ggaacaggag acccacctga ggctcagccc tagccctcag cccacctggg
     1681 gagtttacta cctggggacc ccccttgccc atgcctccag ctacaaaaca attcaattgc
     1741 tttttttttt tggtccaaaa taaaacctca gctagctctg ccaa
//
