LOCUS       NM_173352               2933 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens keratin 78 (KRT78), transcript variant 1, mRNA.
ACCESSION   NM_173352
VERSION     NM_173352.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Langbein,L., Eckhart,L., Fischer,H., Rogers,M.A.,
            Praetzel-Wunder,S., Parry,D.A., Kittstein,W. and Schweizer,J.
  TITLE     Localisation of keratin K78 in the basal layer and first suprabasal
            layers of stratified epithelia completes expression catalogue of
            type II keratins and provides new insights into sequential keratin
            expression
  JOURNAL   Cell Tissue Res 363 (3), 735-750 (2016)
   PUBMED   26340985
  REMARK    GeneRIF: K78 is strongly expressed in the basal and parabasal cell
            layers with decreasing intensity in the lower suprabasal cells of
            keratinising and non-keratinising squamous epithelia and
            keratinocyte cultures.
REFERENCE   3  (bases 1 to 2933)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   4  (bases 1 to 2933)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   5  (bases 1 to 2933)
  AUTHORS   Schweizer,J., Bowden,P.E., Coulombe,P.A., Langbein,L., Lane,E.B.,
            Magin,T.M., Maltais,L., Omary,M.B., Parry,D.A., Rogers,M.A. and
            Wright,M.W.
  TITLE     New consensus nomenclature for mammalian keratins
  JOURNAL   J Cell Biol 174 (2), 169-174 (2006)
   PUBMED   16831889
REFERENCE   6  (bases 1 to 2933)
  AUTHORS   Hesse,M., Zimek,A., Weber,K. and Magin,T.M.
  TITLE     Comprehensive analysis of keratin gene clusters in humans and
            rodents
  JOURNAL   Eur J Cell Biol 83 (1), 19-26 (2004)
   PUBMED   15085952
REFERENCE   7  (bases 1 to 2933)
  AUTHORS   Coulombe,P.A. and Omary,M.B.
  TITLE     'Hard' and 'soft' principles defining the structure, function and
            regulation of keratin intermediate filaments
  JOURNAL   Curr Opin Cell Biol 14 (1), 110-122 (2002)
   PUBMED   11792552
  REMARK    Review article
REFERENCE   8  (bases 1 to 2933)
  AUTHORS   Hesse,M., Magin,T.M. and Weber,K.
  TITLE     Genes for intermediate filament proteins and the draft sequence of
            the human genome: novel keratin genes and a surprisingly high
            number of pseudogenes related to keratin genes 8 and 18
  JOURNAL   J Cell Sci 114 (Pt 14), 2569-2575 (2001)
   PUBMED   11683385
REFERENCE   9  (bases 1 to 2933)
  AUTHORS   Rogers,M.A., Winter,H., Langbein,L., Wolf,C. and Schweizer,J.
  TITLE     Characterization of a 300 kbp region of human DNA containing the
            type II hair keratin gene domain
  JOURNAL   J Invest Dermatol 114 (3), 464-472 (2000)
   PUBMED   10692104
REFERENCE   10 (bases 1 to 2933)
  AUTHORS   Mischke,D. and Wild,G.
  TITLE     Polymorphic keratins in human epidermis
  JOURNAL   J Invest Dermatol 88 (2), 191-197 (1987)
   PUBMED   2433356
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK290901.1, BX647095.1 and
            AC107016.20.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_173352.3.
            
            Summary: This gene is a member of the type II keratin gene family
            and encodes a protein with an intermediate filament domain.
            Keratins are the major structural proteins in epithelial cells,
            forming a cytoplasmic network of 10 to 12 nm wide intermediate
            filaments and creating a scaffold that gives cells the ability to
            withstand mechanical and non-mechanical stresses. The genes of the
            type II keratin family are located as a gene cluster at 12p13.13.
            Four pseudogenes of this gene family have been identified.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK290901.1, ERR279862.1360.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2156670 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304620.5/ ENSP00000306261.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1774              AK290901.1         1-1774
            1775-2666           BX647095.1         1393-2284
            2667-2836           AC107016.20        133515-133684
            2837-2933           BX647095.1         2452-2548
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..2933
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /note="keratin 78"
                     /db_xref="GeneID:196374"
                     /db_xref="HGNC:HGNC:28926"
                     /db_xref="MIM:611159"
     exon            1..448
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1627
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /note="isoform 1 is encoded by transcript variant 1;
                     keratin, type II cytoskeletal 78; keratin-5b;
                     cytokeratin-78; type-II keratin Kb40; keratin 78, type II"
                     /codon_start=1
                     /product="keratin, type II cytoskeletal 78 isoform 1"
                     /protein_id="NP_775487.2"
                     /db_xref="CCDS:CCDS8840.1"
                     /db_xref="GeneID:196374"
                     /db_xref="HGNC:HGNC:28926"
                     /db_xref="MIM:611159"
                     /translation="MSLSPCRAQRGFSARSACSARSRGRSRGGFSSRGGFSSRSLNSF
                     GGCLEGSRGSTWGSGGRLGVRFGEWSGGPGLSLCPPGGIQEVTINQNLLTPLKIEIDP
                     QFQVVRTQETQEIRTLNNQFASFIDKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEP
                     VFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVL
                     KKDVDGVFLSKMELEGKLEALREYLYFLKHLNEEELGQLQTQASDTSVVLSMDNNRYL
                     DFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQ
                     EIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARL
                     LCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGG
                     LGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKK
                     TVESSLKTSITY"
     exon            449..663
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            664..724
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            725..820
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            821..985
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1111
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1332
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1367
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..2933
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1755..1760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /note="hexamer: AATAAA"
     polyA_site      1775
                     /gene="KRT78"
                     /gene_synonym="CK-78; K5B; K78; Kb40"
                     /note="major polyA site"
ORIGIN      
        1 agaaactcac acaccttgac aatctgtctg tccgtctgca gctgcgtgac tgtctgtctc
       61 tgccatgtct ctctccccat gccgggccca gaggggcttc agcgctcgct cagcctgttc
      121 tgctcgctca aggggccgca gcaggggagg cttcagcagc aggggcggct tcagcagcag
      181 gagccttaat tcctttgggg ggtgcctgga aggctctcgt gggagtacct gggggtcagg
      241 gggtaggctg ggggtgcggt ttggggagtg gagtggtggg cctgggctct ccctgtgccc
      301 tccggggggc atccaagaag tgaccatcaa ccagaatctg ctgaccccac tgaagattga
      361 gatcgatccc cagttccagg tggtgcggac gcaggagacc caggagatca gaaccctcaa
      421 caaccagttt gcttccttca ttgacaaggt gcggttcctg gagcagcaga acaaggtcct
      481 ggagacgaag tggcatctgc tgcagcaaca ggggttgagt ggcagccagc agggcctgga
      541 gcctgtcttt gaggcctgcc tggatcagct caggaagcag ctggagcagc tccagggaga
      601 acgaggggct ctggatgctg agttgaaggc ctgccgggac caggaggagg agtataagtc
      661 caagtatgag gaggaggccc acaggcgtgc cacacttgag aacgactttg tggtcctcaa
      721 gaaggatgtg gatggggttt tcctgagcaa gatggagttg gagggcaagc tggaggctct
      781 gagagagtac ctctacttct tgaagcatct gaatgaagaa gagctgggcc agctccagac
      841 ccaggccagc gacacgtctg tggtgctgtc catggacaac aaccgctacc tggacttcag
      901 cagcatcatc actgaggtcc gcgcccggta cgaggagatc gcccggagca gcaaggctga
      961 ggctgaggcc ttgtaccaga ccaagtacca ggaacttcag gtgtctgccc agcttcatgg
     1021 ggacaggatg caggaaacga aagtccagat ctctcagcta caccaagaga ttcagaggct
     1081 gcagagtcag actgagaacc tcaagaagca gaacgccagc ctgcaggccg ccatcactga
     1141 tgctgagcag cgtggggagc tggccctcaa ggacgctcag gccaaggtgg acgagctgga
     1201 ggctgctctg aggatggcca agcagaacct ggcccggctg ctgtgcgagt accaggagct
     1261 gacgagcacg aagctttccc tggatgtgga gattgccact taccgcaggc tgctggaggg
     1321 cgaggagtgc aggatgtctg gggagtgcac cagccaggtc actatctcct cggtgggagg
     1381 cagcgctgtc atgtctggag gagttggtgg aggcttgggg agcacttgtg gactcggtag
     1441 tgggaaaggc agccctgggt cctgctgcac cagcattgtg actggaggct ccaacatcat
     1501 tctgggctct gggaaggacc ctgttttgga ttcctgctct gtgtctggct ccagcgctgg
     1561 ctccagctgc cacaccatcc tgaagaagac agttgagtcg agtctgaaga catccatcac
     1621 ctactgagcg acccagcagc cacctccttc ctgaacacat ttggcccact ccccccatca
     1681 gccggctctg caaggccaac tccgtgtccg ctgcccacag cccaagccag cccacagcgg
     1741 atgctgcaaa aatcaataaa gtctcccctc ctgctgttct gaatgctcta agtgcttgca
     1801 cacctcaccc agcaaaacaa aagctgtgtg gctccccagc ttcctctcct cggaaggcaa
     1861 ttgtggcata tctgtgtcca gacgtgttct attggtatct cactccacat cccagccact
     1921 ctatcaaggg atccttcaag ctctccagtg tgttccttgg ggtgccctcc atctcccaca
     1981 actccactga tgttttagct gtgcttgtag ctcacttcct catcaactgc ctgttttaag
     2041 aggttctagt cctgactggg gcagccccct tccttcttgt tcctccttct tcctgactgt
     2101 gcaccaaaat tttctccttt taggaacctt ctctaattcc catgtctaaa ctactgggcc
     2161 cttcctgtcc ctggcagtct ggctgcacct gttgctgtcc tgggtgctat gtctgtctgg
     2221 ctctggtaag ggactcagct tctctattag cctggcactc ctgcatccag gatgaggatg
     2281 tgctcagctt gtgcctgggg gctctgatgg agattggggt tcaaactctg cccctacccc
     2341 cttatctgtt ggctctcatc ttatccttgg gcaagtgtga acatgaggct ggaaacttct
     2401 cctccttcct ctccttcagg aagctctgca ggaagccagg agtgtgggaa tcaggtgggg
     2461 tgccaggagg ggctggaagg atcacctttt ctacacaccc ccagcagcct catgggtgcc
     2521 tgcagttgct ctttccccat tctcgctttc cagacccagg tgggggacct ggctcaaccc
     2581 caaactccat agtctgggct gcaggaggtc acactacgcc tctccttgct ttagagggag
     2641 ggcagacctt tcctcagatt catgaatcgc tccatccaaa ttatgcaaga aggcttttca
     2701 gctttgtgtg aaggagacca gagccggtga ggatgtggct tgaatcccag ctctgctgtt
     2761 tcctagtggt gtgaccttga tcaagcagct tgacctcttc agttacctca tctgtaaaaa
     2821 gagattaata ataatatgta cctcacaggg ctgtgtggcg acagtgccac taggatccca
     2881 gaaggaaact aaattcatcc aaggtagttc aattaaattg actttttgaa aaa
//
