LOCUS       NM_002265               6058 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens karyopherin subunit beta 1 (KPNB1), transcript variant
            1, mRNA.
ACCESSION   NM_002265
VERSION     NM_002265.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Li,J., Zhang,B., Feng,Z., An,D., Zhou,Z., Wan,C., Hu,Y., Sun,Y.,
            Wang,Y., Liu,X., Wei,W., Yang,X., Meng,J., Che,M., Sheng,Y., Wu,B.,
            Wen,L., Huang,F., Li,Y. and Yang,K.
  TITLE     Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma
            progression through the YBX1-NLGN3 axis
  JOURNAL   J Exp Clin Cancer Res 43 (1), 28 (2024)
   PUBMED   38254206
  REMARK    GeneRIF: Stabilization of KPNB1 by deubiquitinase USP7 promotes
            glioblastoma progression through the YBX1-NLGN3 axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Schertzer,M., Jullien,L., Pinto,A.L., Calado,R.T., Revy,P. and
            Londono-Vallejo,A.
  TITLE     Human RTEL1 Interacts with KPNB1 (Importin beta) and NUP153 and
            Connects Nuclear Import to Nuclear Envelope Stability in S-Phase
  JOURNAL   Cells 12 (24), 2798 (2023)
   PUBMED   38132118
  REMARK    GeneRIF: Human RTEL1 Interacts with KPNB1 (Importin beta) and
            NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in
            S-Phase.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6058)
  AUTHORS   Wang,Y., Xu,J., Zhu,X. and Kuang,H.
  TITLE     MicroRNA-130a-3p impedes the progression of papillary thyroid
            carcinoma through downregulation of KPNB1 by targeting PSME3
  JOURNAL   Endocrine 82 (1), 96-107 (2023)
   PUBMED   37166548
  REMARK    GeneRIF: MicroRNA-130a-3p impedes the progression of papillary
            thyroid carcinoma through downregulation of KPNB1 by targeting
            PSME3.
REFERENCE   4  (bases 1 to 6058)
  AUTHORS   Shi,Q., Lin,M., Cheng,X., Zhang,Z., Deng,S., Lang,K., Yang,Z. and
            Sun,X.
  TITLE     KPNB1-mediated nuclear import in cancer
  JOURNAL   Eur J Pharmacol 955, 175925 (2023)
   PUBMED   37473981
  REMARK    GeneRIF: KPNB1-mediated nuclear import in cancer.
            Review article
REFERENCE   5  (bases 1 to 6058)
  AUTHORS   Okpara,M.O., Hermann,C., van der Watt,P.J., Garnett,S.,
            Blackburn,J.M. and Leaner,V.D.
  TITLE     A mass spectrometry-based approach for the identification of
            Kpnbeta1 binding partners in cancer cells
  JOURNAL   Sci Rep 12 (1), 20171 (2022)
   PUBMED   36418423
  REMARK    GeneRIF: A mass spectrometry-based approach for the identification
            of Kpnbeta1 binding partners in cancer cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6058)
  AUTHORS   O'Neill,R.E., Jaskunas,R., Blobel,G., Palese,P. and Moroianu,J.
  TITLE     Nuclear import of influenza virus RNA can be mediated by viral
            nucleoprotein and transport factors required for protein import
  JOURNAL   J Biol Chem 270 (39), 22701-22704 (1995)
   PUBMED   7559393
REFERENCE   7  (bases 1 to 6058)
  AUTHORS   Chi,N.C., Adam,E.J. and Adam,S.A.
  TITLE     Sequence and characterization of cytoplasmic nuclear protein import
            factor p97
  JOURNAL   J Cell Biol 130 (2), 265-274 (1995)
   PUBMED   7615630
REFERENCE   8  (bases 1 to 6058)
  AUTHORS   Gorlich,D., Kostka,S., Kraft,R., Dingwall,C., Laskey,R.A.,
            Hartmann,E. and Prehn,S.
  TITLE     Two different subunits of importin cooperate to recognize nuclear
            localization signals and bind them to the nuclear envelope
  JOURNAL   Curr Biol 5 (4), 383-392 (1995)
   PUBMED   7627554
REFERENCE   9  (bases 1 to 6058)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
REFERENCE   10 (bases 1 to 6058)
  AUTHORS   Mukaigawa,J. and Nayak,D.P.
  TITLE     Two signals mediate nuclear localization of influenza virus
            (A/WSN/33) polymerase basic protein 2
  JOURNAL   J Virol 65 (1), 245-253 (1991)
   PUBMED   1985200
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025682.6, AC015674.12 and
            KF570270.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002265.5.
            
            Summary: Nucleocytoplasmic transport, a signal- and
            energy-dependent process, takes place through nuclear pore
            complexes embedded in the nuclear envelope. The import of proteins
            containing a nuclear localization signal (NLS) requires the NLS
            import receptor, a heterodimer of importin alpha and beta subunits
            also known as karyopherins. Importin alpha binds the NLS-containing
            cargo in the cytoplasm and importin beta docks the complex at the
            cytoplasmic side of the nuclear pore complex. In the presence of
            nucleoside triphosphates and the small GTP binding protein Ran, the
            complex moves into the nuclear pore complex and the importin
            subunits dissociate. Importin alpha enters the nucleoplasm with its
            passenger protein and importin beta remains at the pore.
            Interactions between importin beta and the FG repeats of
            nucleoporins are essential in translocation through the pore
            complex. The protein encoded by this gene is a member of the
            importin beta family. Two transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Feb
            2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L38951.1, SRR1660805.130913.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290158.9/ ENSP00000290158.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-366               AC025682.6         6489-6854           c
            367-425             AC025682.6         6329-6387           c
            426-608             AC025682.6         3897-4079           c
            609-809             AC015674.12        139928-140128       c
            810-962             AC015674.12        138328-138480       c
            963-1022            AC015674.12        135810-135869       c
            1023-1112           AC015674.12        133810-133899       c
            1113-1223           AC015674.12        132719-132829       c
            1224-1325           AC015674.12        131830-131931       c
            1326-1550           AC015674.12        128578-128802       c
            1551-1742           AC015674.12        127119-127310       c
            1743-1873           AC015674.12        126156-126286       c
            1874-2021           AC015674.12        123823-123970       c
            2022-2093           AC015674.12        123427-123498       c
            2094-2238           AC015674.12        122206-122350       c
            2239-2321           AC015674.12        120497-120579       c
            2322-2429           AC015674.12        119861-119968       c
            2430-2573           AC015674.12        118799-118942       c
            2574-2679           AC015674.12        118575-118680       c
            2680-2794           AC015674.12        116854-116968       c
            2795-2956           AC015674.12        116319-116480       c
            2957-3324           AC015674.12        114217-114584       c
            3325-3565           KF570270.1         1-241               c
            3566-6058           AC015674.12        111483-113975       c
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..6058
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="karyopherin subunit beta 1"
                     /db_xref="GeneID:3837"
                     /db_xref="HGNC:HGNC:6400"
                     /db_xref="MIM:602738"
     exon            1..366
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..2957
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="isoform 1 is encoded by transcript variant 1;
                     importin 1; nuclear factor p97; importin 90; importin
                     beta-1 subunit; importin subunit beta-1; PTAC97; pore
                     targeting complex 97 kDa subunit; karyopherin (importin)
                     beta 1"
                     /codon_start=1
                     /product="importin subunit beta-1 isoform 1"
                     /protein_id="NP_002256.2"
                     /db_xref="CCDS:CCDS11513.1"
                     /db_xref="GeneID:3837"
                     /db_xref="HGNC:HGNC:6400"
                     /db_xref="MIM:602738"
                     /translation="MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVL
                     ANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTE
                     TYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQD
                     IDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFI
                     MQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVA
                     LQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQD
                     ENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGC
                     ILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLA
                     PLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQ
                     KLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMES
                     HIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGV
                     QEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQ
                     SNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQ
                     QASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFIL
                     SFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTN
                     KAKTLATWATKELRKLKNQA"
     exon            367..425
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            426..608
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            609..809
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            810..962
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1022
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1112
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1223
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1325
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1550
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1742
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1873
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2021
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2093
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2238
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2321
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2429
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2430..2573
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2679
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2794
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2795..2956
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..6058
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5192..5197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="hexamer: AATAAA"
     polyA_site      5223
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="major polyA site"
     regulatory      6030..6035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="hexamer: AATAAA"
     polyA_site      6058
                     /gene="KPNB1"
                     /gene_synonym="IMB1; Impnb; IPO1; IPOB; NTF97"
                     /note="major polyA site"
ORIGIN      
        1 ccctccctgc gcgccgcctc tcactcacag cctcccttcc ttctttctcc ctccgcctcc
       61 cgagcaccag cgcgctctga gctgccccca gggtccctcc cccgccgcca gcagcccatt
      121 tggagggagg aagtaaggga agaggagagg aaggggagcc ggaccgacta cccagacaga
      181 gccggtgaat gggtttgtgg tgacccccgc cccccacccc accctccctt cccacccgac
      241 ccccaacccc catccccagt tcgagccgcc gcccgaaagg ccgggccgtc gtcttaggag
      301 gagtcgccgc cgccgccacc tccgccatgg agctgatcac cattctcgag aagaccgtgt
      361 ctcccgatcg gctggagctg gaagcggcgc agaagttcct ggagcgtgcg gccgtggaga
      421 acctgcccac tttccttgtg gaactgtcca gagtgctggc aaatccagga aacagtcagg
      481 ttgccagagt tgcagctggt ctacaaatca agaactcttt gacatctaaa gatccagata
      541 tcaaggcaca atatcagcag aggtggcttg ctattgatgc taatgctcga cgagaagtca
      601 agaactatgt tttgcagaca ttgggtacag aaacttaccg gcctagttct gcctcacagt
      661 gtgtggctgg tattgcttgt gcagagatcc cagtaaacca gtggccagaa ctcattcctc
      721 agctggtggc caatgtcaca aaccccaaca gcacagagca catgaaggag tcgacattgg
      781 aagccatcgg ttatatttgc caagatatag acccagagca gctacaagat aaatccaatg
      841 agattctgac tgccataatc caggggatga ggaaagaaga gcctagtaat aatgtgaagc
      901 tagctgctac gaatgcactc ctgaactcat tggagttcac caaagcaaac tttgataaag
      961 agtctgaaag gcactttatt atgcaggtgg tctgtgaagc cacacagtgt ccagatacga
     1021 gggtacgagt ggctgcttta cagaatctgg tgaagataat gtccttatat tatcagtaca
     1081 tggagacata tatgggtcct gctctttttg caatcacaat cgaagcaatg aaaagtgaca
     1141 ttgatgaggt ggctttacaa gggatagaat tctggtccaa tgtctgtgat gaggaaatgg
     1201 atttggccat tgaagcttca gaggcagcag aacaaggacg gccccctgag cacaccagca
     1261 agttttatgc gaagggagca ctacagtatc tggttccaat cctcacacag acactaacta
     1321 aacaggacga aaatgatgat gacgatgact ggaacccctg caaagcagca ggggtgtgcc
     1381 tcatgcttct ggccacctgc tgtgaagatg acattgtccc acatgtcctc cccttcatta
     1441 aagaacacat caagaaccca gattggcggt accgggatgc agcagtgatg gcttttggtt
     1501 gtatcttgga aggaccagag cccagtcagc tcaaaccact agttatacag gctatgccca
     1561 ccctaataga attaatgaaa gaccccagtg tagttgttcg agatacagct gcatggactg
     1621 taggcagaat ttgtgagctg cttcctgaag ctgccatcaa tgatgtctac ttggctcccc
     1681 tgctacagtg tctgattgag ggtctcagtg ctgaacccag agtggcttca aatgtgtgct
     1741 gggctttctc cagtctggct gaagctgctt atgaagctgc agacgttgct gatgatcagg
     1801 aagaaccagc tacttactgc ttatcttctt catttgaact catagttcag aagctcctag
     1861 agactacaga cagacctgat ggacaccaga acaacctgag gagttctgca tatgaatctc
     1921 tgatggaaat tgtgaaaaac agtgccaagg attgttatcc tgctgtccag aaaacgactt
     1981 tggtcatcat ggaacgactg caacaggttc ttcagatgga gtcacatatc cagagcacat
     2041 ccgatagaat ccagttcaat gaccttcagt ctttactctg tgcaactctt cagaatgttc
     2101 ttcggaaagt gcaacatcaa gatgctttgc agatctctga tgtggttatg gcctccctgt
     2161 taaggatgtt ccaaagcaca gctgggtctg ggggagtaca agaggatgcc ctgatggcag
     2221 ttagcacact ggtggaagtg ttgggtggtg aattcctcaa gtacatggag gcctttaaac
     2281 ccttcctggg cattggatta aaaaattatg ctgaatacca ggtttgtttg gcagctgtgg
     2341 gcttagtggg agacttgtgc cgtgccctgc aatccaacat catacctttc tgtgacgagg
     2401 tgatgcagct gcttctggaa aatttgggga atgagaacgt ccacaggtct gtgaagccgc
     2461 agattctgtc agtgtttggt gatattgccc ttgctattgg aggagagttt aaaaaatact
     2521 tagaggttgt attgaatact cttcagcagg cctcccaagc ccaggtggac aagtcagact
     2581 atgacatggt ggattatctg aatgagctaa gggaaagctg cttggaagcc tatactggaa
     2641 tcgtccaggg attaaagggg gatcaggaga acgtacaccc ggatgtgatg ctggtacaac
     2701 ccagagtaga atttattctg tctttcattg accacattgc tggagatgag gatcacacag
     2761 atggagtagt agcttgtgct gctggactaa taggggactt atgtacagca tttgggaagg
     2821 atgtactgaa attagtagaa gctaggccaa tgatccatga attgttaact gaagggcgga
     2881 gatcgaagac taacaaagca aaaacccttg ctacatgggc aacaaaagaa ctgaggaaac
     2941 tgaagaacca agcttgatct gttaccattg ggatgataac ctgaggaccc ccactggaaa
     3001 tctcccatct tttgaaaaac ctggaagtga ggagtgtgca cggatgctga atgtttggga
     3061 atgagaggat gagtgagtga ggcttgaaaa cacaccacat tgaaaatcct gccacagcag
     3121 cagccgcagc cgccaacagc agcgctgtta gtgagctaag taagcactga cttcgtagaa
     3181 aaccataaca tcggccatct tggaaaagag aaaaacaatg gagttactta tttaaaaaaa
     3241 aagaaagaaa gttatctctt cccaggagag gctagaagta gcttttctgt cttttggcca
     3301 gtgccgagtg gaatgcctgg tttgggggag gaggagggac tgggttcagc tgtggtgctt
     3361 tgttgtaaaa ggcagcctgg cctttgctac tgaggagaaa gatggagcct gggtctcaag
     3421 cccaccttcg ctgtaccttt gccacatggt actgtatgct tgccagctag aaggagggtc
     3481 agggattttt tacagtctga gaatgagtgt gtgtgagtga ggcggtatcc acattctcaa
     3541 cttcaagtca ttgcagtttc tttttcccag aaaacaaggg gttagatgtt gcatttcata
     3601 aaactaaccg aagttttgtt tactgatgca gcacaagaga tgtaaaaaaa aaaaaaaaaa
     3661 aaaaaaaaaa aacacacaca cagaggaaag acgctcttta ggttttgttt tgtttttttt
     3721 ttttggtttt gttttttgtt ttttttactc tagggaaaac actgacgaat ggtcagagct
     3781 cctatcctga tcttttcatc aaggcgcctt tcctaataat atggttcaac tgtgaatgta
     3841 gaagtggggg ggagggggga gaaaaagaaa actctggcgt tagaggatat agaaaaatat
     3901 aagtacaatt gttacaaata acgcagactt caaaaacaaa aaaatcacaa cccaaacaaa
     3961 ccaaaattta aatgatcaga attggcagca caaagaaaac gccctctcct gacttgtatt
     4021 gtggcagtct gaacgccccc agaaaattgt gccaaagagt ttagaaaaat aaatatacaa
     4081 taaaagtaaa cacatacaca caaaacagca aacttcaggt aactattttg gattgcaaac
     4141 aggataaatt aaatgttcaa acaatctgat aaaataacca tttggaaact gcttggcctt
     4201 ctgttctttt atttgattga ctacaatgcg gtattggtct cttgctgcac ttcaaaagca
     4261 accaacaaaa caaaaacaaa aaaaagtgtg tgttgtgtga atacacacac acactaacta
     4321 gaagtcttgt gatgaaaatg gcacttggaa aaggttttat ttttccactg aagttgaagg
     4381 ttaataaaat ggtgtcaaac gtcccctggt cacacacttg aatatttttt tagaagtgtg
     4441 atgtggtatg attaccataa atcagactta attattttcc cttttacaag ggaacagggc
     4501 atcctgaatt ttagagcctt tcagcaataa gaagggatgt tggtgagctt tgatcctctt
     4561 ttggttttgc agttgttagg agtttttgct ggcattttga atatgctgct ttcagaaaaa
     4621 ccaaggaagt tttaaaattg cttcctggtc cttagaggac taaaaacaag accctcattc
     4681 ccactttcat ttccactcta gcaaaaactg ggcttgcgtt tttctccaac tcctcgttta
     4741 tatcctccct tccatgtcca agccttccat tcctaagtgg gattggctca gttttgccca
     4801 tccatatggc agcatctcta atagctcttg tacagggtat cagatattgt gccttttggt
     4861 gccaggttca aagtcaagtg ccgatctatg aaccagtgta caaaaaaaaa aaaatccagg
     4921 tatttgaagg agagacgctc cattgtgaat aaagagctca taccagctcc taagccctat
     4981 taagaagagg cctggtcctc taatgccttg tttccatttc agttgttctt tgagagacag
     5041 aatgatgtac taaccattcg tgattattaa gatagggttg ggtcagggct tagggagggg
     5101 gcagaaatat tggggataga aaaaaaatct gatcattcct cagtgctacc catttctgtc
     5161 ctgtgtgggc tgcttagcta gacagcagga gaataaagta caccgagaac cataatgaaa
     5221 aaaccttccg tgtgttttgt catgttttgt tccagggaag cagttgatga gtgctgttac
     5281 taatgctttc tcccagatcc attcagtggt ggagaggagg aaaatgggct ggttggatgt
     5341 ggtcttggtg ccttgcagtt actctgcact ggttatgcat ttaattctcc tcttttctag
     5401 ttaacctttt gccagtgggt tttccatagt ctgggtattt gtccttatat cagttatacc
     5461 acctaaggca actgggtgca aaatgcattc tgttcactca ctgtctgggc cttccccacc
     5521 ctagtcttgg cacattcctt caagaatgta gttaccgtct gcttgggaag atgtcagtgc
     5581 aaatgtgaag ataatgggca tcggactagg ctttggtttg ccacaagtgg cagctgcctg
     5641 tatcaattac atttattaat tttgcttttc atttttccca gttctcctca cccccccttt
     5701 gttgaaatgt tggacttgct gtcagaggca actgtaatat tgccttaggg acttgtggag
     5761 aaggggaatt gctcagtgta gtgttttaac tttcagaacc aagcaatcta tttactctta
     5821 caaatattta agaagtgtgt tagtccaact ttaagaaaaa tccaaactca tcagcttttg
     5881 atagcatctt ggtttttgaa acaacttcaa tctgtaattg gcattcagaa tgcccttggc
     5941 atgccagtct gtgatggcat ttaagacctg taaaacactt gagcccaact cgattaacca
     6001 aaaccgataa ccaccacctt tatcttctaa ataaagtccg ctttattttt attttcaa
//
