LOCUS       NM_001366306           11288 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens karyopherin subunit alpha 5 (KPNA5), transcript
            variant 4, mRNA.
ACCESSION   NM_001366306 XM_017010839
VERSION     NM_001366306.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 11288)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 11288)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11288)
  AUTHORS   Sun,M., Long,J., Yi,Y. and Xia,W.
  TITLE     Importin alpha-importin beta complex mediated nuclear translocation
            of insulin-like growth factor binding protein-5
  JOURNAL   Endocr J 64 (10), 963-975 (2017)
   PUBMED   28835592
  REMARK    GeneRIF: Data suggest that IGFBP5 nuclear import is mediated by
            KPNA5/KPNB1 complex; nuclear localization sequence of IGFBP5 is
            critical domain in this nuclear translocation. (IGFBP5 =
            insulin-like growth factor binding protein-5; KPNA5 = karyopherin
            subunit alpha-5; KPNB1 = karyopherin subunit beta-1/importin-beta)
REFERENCE   6  (bases 1 to 11288)
  AUTHORS   Di Marzio,P., Choe,S., Ebright,M., Knoblauch,R. and Landau,N.R.
  TITLE     Mutational analysis of cell cycle arrest, nuclear localization and
            virion packaging of human immunodeficiency virus type 1 Vpr
  JOURNAL   J Virol 69 (12), 7909-7916 (1995)
   PUBMED   7494303
REFERENCE   7  (bases 1 to 11288)
  AUTHORS   Gallay,P., Swingler,S., Song,J., Bushman,F. and Trono,D.
  TITLE     HIV nuclear import is governed by the phosphotyrosine-mediated
            binding of matrix to the core domain of integrase
  JOURNAL   Cell 83 (4), 569-576 (1995)
   PUBMED   7585960
REFERENCE   8  (bases 1 to 11288)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   9  (bases 1 to 11288)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
REFERENCE   10 (bases 1 to 11288)
  AUTHORS   Sharova,N. and Bukrinskaya,A.
  TITLE     p17 and p17-containing gag precursors of input human
            immunodeficiency virus are transported into the nuclei of infected
            cells
  JOURNAL   AIDS Res Hum Retroviruses 7 (3), 303-306 (1991)
   PUBMED   2064827
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL132795.12, KF458260.1 and
            AL354716.9.
            
            On May 17, 2019 this sequence version replaced NM_001366306.1.
            
            Summary: The transport of molecules between the nucleus and the
            cytoplasm in eukaryotic cells is mediated by the nuclear pore
            complex (NPC) which consists of 60-100 proteins and is probably 120
            million daltons in molecular size. Small molecules (up to 70 kD)
            can pass through the nuclear pore by nonselective diffusion; larger
            molecules are transported by an active process. Most nuclear
            proteins contain short basic amino acid sequences known as nuclear
            localization signals (NLSs). KPNA5 protein belongs to the importin
            alpha protein family and is thought to be involved in NLS-dependent
            protein import into the nucleus. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (4), as well as variant 3, encodes
            isoform b.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.86976.1,
                                           SRR1660807.265189.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368564.7/ ENSP00000357552.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               AL132795.12        75050-75177
            129-262             AL132795.12        83159-83292
            263-364             AL132795.12        85894-85995
            365-464             AL132795.12        86132-86231
            465-559             AL132795.12        92543-92637
            560-691             AL132795.12        95858-95989
            692-780             AL132795.12        98911-98999
            781-880             AL132795.12        110058-110157
            881-1044            AL132795.12        115965-116128
            1045-1064           AL132795.12        118136-118155
            1065-1123           KF458260.1         330-388             c
            1124-1249           AL132795.12        119590-119715
            1250-1377           AL132795.12        120334-120461
            1378-1556           AL132795.12        123402-123580
            1557-10999          AL132795.12        125975-135417
            11000-11288         AL354716.9         101-389
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.1"
     gene            1..11288
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /note="karyopherin subunit alpha 5"
                     /db_xref="GeneID:3841"
                     /db_xref="HGNC:HGNC:6398"
                     /db_xref="MIM:604545"
     exon            1..128
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1744
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /note="isoform b is encoded by transcript variant 4;
                     importin alpha 6; importin subunit alpha-6; karyopherin
                     alpha 5 (importin alpha 6)"
                     /codon_start=1
                     /product="importin subunit alpha-6 isoform b"
                     /protein_id="NP_001353235.1"
                     /db_xref="CCDS:CCDS5111.1"
                     /db_xref="GeneID:3841"
                     /db_xref="HGNC:HGNC:6398"
                     /db_xref="MIM:604545"
                     /translation="MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKRE
                     EQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLT
                     ATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTF
                     LHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLL
                     ELLTNSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVLADVC
                     WALSYLSDGPNDKIQAVIDSGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQV
                     ILNCSALPCLLHLLSSPKESIRKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQK
                     AEFRTRKEAAWAITNATSGGTPEQIRYLVALGCIKPLCDLLTVMDSKIVQVALNGLEN
                     ILRLGEQESKQNGIGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVE
                     EDDPSIVPQVDENQQQFIFQQQEAPMDGFQL"
     exon            129..262
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            263..364
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            365..464
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            465..559
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            560..691
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            692..780
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            781..880
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1044
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1123
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1249
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1377
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1556
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..11288
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5040..5045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /note="hexamer: AATAAA"
     polyA_site      5065
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /note="major polyA site"
     regulatory      11266..11271
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
                     /note="hexamer: AATAAA"
     polyA_site      11288
                     /gene="KPNA5"
                     /gene_synonym="IPOA6; SRP6"
ORIGIN      
        1 atcttggatt gcgaactggg tcgctacgct tcacgccagg ggcggagtgg cggcccttct
       61 gttacccgcc acacacgtcg ccgctgggga ctgggaaatc agggcatcgg agagtgccac
      121 attaatggat gccatggcta gtccagggaa agataactat agaatgaaaa gttataagaa
      181 taaagcccta aatcctcaag agatgcgtag acgaagagaa gaagaaggaa tacagcttag
      241 aaaacaaaaa agagaagaac agttgttcaa acgcagaaat gtctatttgc ccagaaatga
      301 tgaatctatg cttgaaagtc ctatacagga tccagatatt agttccactg tacccattcc
      361 agaggaagaa gttgttacta cagatatggt tcagatgatt ttttctaata atgctgatca
      421 acagctaaca gcaacacaga aatttagaaa gctgctttct aaagaaccta atccaccaat
      481 agatcaagtt atacagaaac caggagttgt acagagattt gtgaaatttc ttgaaagaaa
      541 tgaaaattgc actttacaat ttgaagctgc atgggcatta acaaatatag catctggaac
      601 ttttctgcat accaaggtag tgattgaaac tggggctgtt ccgattttta tcaaacttct
      661 taattctgaa catgaagatg ttcaggaaca ggctgtttgg gcacttggta atattgctgg
      721 tgacaatgca gaatgcagag attttgtttt gaattgtgaa atacttccac ctcttttaga
      781 gttattaaca aattcaaaca gactcacaac aacaagaaat gccgtgtggg ccctctcaaa
      841 tttatgtaga ggcaaaaacc ctcctccaaa ctttagtaag gtttcacctt gcttaaatgt
      901 cctgtcacga ctgttgttta gcagtgaccc agatgtgtta gcagacgtgt gttgggccct
      961 ttcttatctc tccgatggac ccaatgataa aattcaagca gtcattgatt ctggagtctg
     1021 tcgaagattg gtggaacttt tgatgcacaa tgattataaa gttgtatcac ctgcattaag
     1081 ggcagttggt aatattgtga ctggtgatga tattcaaaca caggtaattt tgaattgttc
     1141 tgcattaccc tgtctcttac atttattgag tagcccaaag gagtcaatta gaaaagaagc
     1201 ctgctggact gtttctaaca tcactgctgg aaatagagct cagattcagg ctgttataga
     1261 tgcaaatatt tttcctgttt tgattgagat tcttcagaaa gcagagtttc gtaccagaaa
     1321 agaagcagct tgggctataa ctaatgcaac atcaggaggt actccagagc aaataaggta
     1381 tttggtagct ttaggctgca ttaaaccact ttgtgatctt ttgactgtta tggactccaa
     1441 aatagtccaa gtggctttaa atggacttga aaatatttta cgtcttggag aacaagaatc
     1501 taagcagaat ggaataggca ttaatccata ctgtgctctc attgaagaag catatggtct
     1561 ggataaaatt gagtttttgc aaagccatga aaatcaggaa atttaccaga aggcatttga
     1621 tctgattgaa cattactttg gtgtagaaga agatgacccc agcattgtac ctcaggtgga
     1681 tgaaaaccaa caacagttta tatttcagca gcaggaagca ccaatggatg gatttcaact
     1741 ttaacttact ggaggaaaaa aaatttatgg ctaaaaaggg tagcttcagg taactcctct
     1801 ttgttgccaa tgtaagaatg tttgtttttt tacatagaac agtaaagaga atttgatgca
     1861 cttttagaaa gcaaaatgaa acaaaaattt ccattcagat gcaacctttc attgtagttt
     1921 gttgttgttt tagttttgtt gttgtgcctg tatttatatc ttctattgtt tggatttttg
     1981 tatctatttg tgaataattg aatatgcaat tatttagtaa tttaagcttg aaaaggagaa
     2041 ttttggtgaa gcattactta gtaattctga attttttcaa gcattcttgg aaactgcaca
     2101 ttagcagtaa acctattgat aattgcattt tggcagtaag tcttacacat ctaatctcta
     2161 ctaaatctac ctctatcttg aagcagaaat aaaaacaaaa aagcttcaga agcatgaaat
     2221 aatgcaaatg ctaaattaga atgtgaaaat atttattacc ttaaattata catatcactg
     2281 tgctgtaggt tatactttgc aaaaaattgc aacagaaaac ctataaaatt tatttataaa
     2341 acagaagaaa tattatactg ataatccatt aaaatgtggc aattgtgaag ggagaagctg
     2401 tttttcatgt tgaacacatt aaaaagatta ctttataaaa tgtttaaata tttctgtttt
     2461 taacatataa gtgctatgta tattagtata ttttgtattt caaccaaata tgctaccatt
     2521 tttgaaatag tgttttattg tttttcactc attgttttaa agagcatcat gacagagatg
     2581 tcatcatgaa tctaaagtag tgctgcatat ctaaaataat aactaattat ttctctgatt
     2641 tttcagagca gtaattgaaa agtttcattt tctgtataat agtactagtt taagtttaag
     2701 tacaagtaca agtttaggta ctcgaatgac aaaattaccc tgaagaaata accttattat
     2761 gtgttaaatt gtattaaatg catttaacat ttggatgcta aatgataatg aaaattgcta
     2821 aaggttcttt aatatggttc aaatctataa gacttttttc ttttagtaat actcagaggg
     2881 tcattctgct gcttgttatg aatcatagat tacattcatg tcagattgat caatatgtat
     2941 gttttatgat ttaacagcaa aaacctcttc aaacggaaaa tttgaaagaa aggtttcaat
     3001 agaaaaatta tatatatatt tatacatata tatgtgtgtg tgttactctc acagttcaac
     3061 tactgttgaa ctcaatcttt taatttatag ttatacgtag gctatttatg tgtccaattg
     3121 tatacctaga atacttactt aaaacttagt ttacaactct ttttaagatg agaactaagg
     3181 caattttgat atgattcatg gtctattctt taaaaaaaga tttcatttat gatagtattt
     3241 gtaatgtttc tgctggaaat cagaggcacg ttccaagagg aaaatgtata taggagtact
     3301 tttaaagaat atgtcacttt gtaaattaca tatcatgaaa ctaagctttt gacaagatac
     3361 ttaaaaccta cagctgtaaa atacaggttt aaatggtgtt cgtaatgctg agtgcatctg
     3421 ccagcccttt tttattatta agtagaagat tgtatctgtg cccctttttt cgtagtggaa
     3481 ggtatatagc cgagtataaa atggttttct tttgcattat ctgtattcaa aaaacagggt
     3541 agttatatta aagcttacca aattgatggt gaatttatca gattaacctt tttgtggtgc
     3601 agatggttct aagtatcagg tgacagggta ttctactctt tctagtcaat taaaattgat
     3661 aatgcagcat gattcataaa ctaaccagct gtgcctcctt ttattttgag gaccctgtag
     3721 tcactataat aaatttctaa gccaaatttt tcaaacaaga tagttcttat agaaggagaa
     3781 ataataaata tgtgtgtgtt gatgcatgca cacagaatga cctgtgaaag gaaacttaaa
     3841 ttttgccggt aaaacaaaca tatctctaca gactgtgatt atacttcata agcatttaaa
     3901 tacagtttta tttatttcct taaatatatt acttgggtaa ttaattttag atttgattgc
     3961 aaagagcact gtatagcagt tgggaccagc tcttggcttg gcttggcttg gcttattgtt
     4021 aagagtttat tggatcaaga ctaacaaata actttgtgat gaaaattctt caaaaatata
     4081 gctactcttt tcaagtatac catttaaaat atttcatcag gcagagccct gaccaggaaa
     4141 aaaaaaaaaa aagaaagaaa gaaaagaaaa aaataaataa aatgctccaa aaagtgtttt
     4201 cagtagtctt cactggccct tgtgggagga aaaatacttt tttaacttgc ttttctaaga
     4261 tttgttatat ttaaatccaa gagaaactat gccgaaaaaa agtgttataa agaggaatgc
     4321 atgtagcatt aatgcaaata tcaagaaaat acttgaaaga gtccttttca tttatttaat
     4381 taaatttggt gttttatttt gtctgaatct gaatttctcc tatttaatct atgcaattat
     4441 gcttgtttta tatttataag cacttgactc actgggtgtg gtctttttac tgtgttttat
     4501 ttcaaaacgt tgttttaagt gatagtgtcc ttaaatttta gtgtcatttg gtcaacttac
     4561 tggtaacacc agaacaagaa agcaaaaaag taataaattc tgctggcaca gaactaacat
     4621 atcatttggt aactgtaaaa cctatttgca gttaaaggtt gatttattgg ataagggact
     4681 aaaagaagca tatattacct ttgcctttat attttttctt cttttaagaa ggagcattat
     4741 ggaaaaataa atataaagtc ttagtgaatt tttgttatgg ttctcaattc agcaatctac
     4801 tggctgcttg cgaaactgtg aaatcaaatt gttcttaacc tttattcagt ttagtttata
     4861 gcattactgt tttatgaaat gtgacatttt aggagccaat ataatagagc tctaaaagga
     4921 tagtatatct ttaaattctg actggggagt aagaagagaa gatattctta ccctactaaa
     4981 taaaccaact tttgagtagt atgcaatatt atgaaatgtt acaacactat attaaaaata
     5041 ataaaatatt ttaaaattgc atttatataa atgtgtccat ttcatttttt cttaagattg
     5101 ttgtttccta acagaacaaa gacagatgct attattggtt aaaaatccat ttactttcta
     5161 aaactaacaa aggagaaaaa atagaattat aaaaaatagc taatccaaaa ctagacagaa
     5221 aaagagaaaa aacaaatgaa gatggaacaa atagcaagaa agatcaaact cagttatatc
     5281 agtagtcaca gtaattacta atgatttaaa catgccaatt aaagtgcaga ggttatcagt
     5341 tttaataaaa aagtaaggcc gtattttgcc tctaagaaaa tgttttcaag aagcaaattg
     5401 gtgaaaaggg tggaaaaaag atataccatg ctatcacaaa tcaaaagtaa gttggggtgg
     5461 ccatataatg catatcaagg tgcatttcag agcaaggaat atttattgct ggagataaag
     5521 agggtcattt gataaaggga tcaattcatg atagtcaaaa tgtagaaaca aatgttcatc
     5581 agccaatgga taaataaaat gttatattca taaaatggga tattactcag taataaaatg
     5641 aaatatggat atatggtaca acatggatga acattgaaaa cattaggcta agtgaaagaa
     5701 accagattcc atttatatga agtgttcgga atagacaaat ctatagaaac agaaataaat
     5761 tagtatctag gactggggaa agttggaaat gggaggtgac tgggtacaaa gtttcttctt
     5821 tgagcaataa aaataggatt ttctaataaa ttcagagatg tgctgccatc accataagca
     5881 aaagttcact tttttactgg cactagccat atttcaagtg ctcagtaggg agtgcagggc
     5941 tacagtatgt tttttaaatc acgtgagagt aacacaaagt tttaaatgca tattttacac
     6001 cccaaagcaa ccaattcttg ttcttggagt tagctgagct tcctaggatc catggattta
     6061 tagttttcat caaatttgaa aacacagcaa ttatttaaac attttttcat ttccaattcc
     6121 gcttatatta gtctgcctga aatactcaca cagcttatta atgctttgct catttttggt
     6181 tatttttcat tttgtatagt tttcattgtt tcgtcttcaa gttcactaat cttgtcttct
     6241 gctgtgtcta atctcttaat tccattagtg tattttttat gtcagacatt gtgtatttta
     6301 tctttaaaaa ttcaatttgg atctgtttta tatcttccat tttttttatc atactcaagc
     6361 tttcctctgc tggcttgaac acagcaagta taatagctgt ttgaatggcc ttgctactaa
     6421 ttctgtcatt tcttgctctg tttctgttta ttgatttttt tattatgagt tgtatttttc
     6481 atttgcatgc gtttacatgt ttggatattg aatttcccat atttgttctt cttaacacaa
     6541 gttactaggg gaaaaaatgt gatcctttaa aggcttgctt ttaaactttg taaggcaggc
     6601 acagaacaac gtttatttta ctgctgagtc agtaccattc cgagtactct attaccctat
     6661 gaattacaag ggattttcac tttggctagt ggaacttaaa ctgttcctga tcattgtggg
     6721 ctccaaaaat tgtttcccct tgtgctttca ggtgattctt tagaaggcct tgggtagtag
     6781 tcacatgtgg tcaatagtac ttggctgaag actcaaaggg gaatcctctg cagataatct
     6841 ggagcaatct gtgtagtttt gtcacatttg gtactctacc ttgtgaactc tagttttgtc
     6901 ctccctggct tcctaactct ttctccccat ctcagagact ttgtggctat acctgaattt
     6961 cccttccttg ccctgcaatc gtgaaactct aggcagtaag agacagagca gtcatagggc
     7021 tcaactagtt tgtttcctct gtttcataga tcacggttcc tcattatctg atgtctaatg
     7081 tctggaaact gttgtttcat gtttttgtcc tggtccctat tactctagtt tggcttaaag
     7141 cagaagttag cataccaatc tatgttcttt ttaaaatctc tgtttaatgt gggctttgat
     7201 catttttcat attgttttct tggtgccttt ttgtctcaat cactttcgct gaattcagtt
     7261 atagtgatta tagtcttttt acttatttcc ttagtttttg ctcataattt tagaatttcc
     7321 aatcttttat tctctttgga tttattctta agtctttccc taatttccct aaacatttaa
     7381 ggttgttaaa tgtttaattt gggaaggcta aaaatttacc ttgaagtacc atttcagctg
     7441 cattccacaa gttattcagc tctaagtatt ttaaagttta tattatgatt tattcgtgac
     7501 ctatgaaaat tgacagaccg ttagcaagac taataaagaa gaaaagagag aagaatcaaa
     7561 tagacgcaat aaaaaatgat aaaggggata tcaccaccga tcccacagaa atacaaacta
     7621 ccatcagaga atactataaa cacctctacg caaatatact agaaaatcta gaagaaatgg
     7681 ataaattcct cgacacataa accctcccaa gactaaacca ggaagaagtt gaatctctga
     7741 atagaccaat aacaggatct gaaattgtgg caataatcaa tagcttacca acaaaaagag
     7801 tccaggacca gatggattca cagctgaatt ctaccagagg tacaaggagg aactggtacc
     7861 attccttctg aaactattcc aatcaataga aaaagaggga atcctcccta actcattttc
     7921 tgaggccagc atcatcctga taccaaagct gggcagagac acaaccaaaa aagagaattt
     7981 tagaccaata tccttggtga acattgatgc aaaaatcctc aataaaatac tggcaaaccg
     8041 aatccagcag cacatcaaaa agcttatcca ccatgatcaa gtgggcttca tccctgggat
     8101 gcaaggctgg ttcaatatac gcaaatcaat aaatgtaatc cagcatataa acagaaccaa
     8161 agacaaaaaa cacatgatta tctcaacaaa tgcagaaaag gccttcgaca aaattcaaca
     8221 acgcttcatg ctaaaaaact ctcaataaat taggtattga tgggacgtat ctccaaataa
     8281 taagagctat ctatgacaaa cccacagcca atatcatact gaatgggcaa aaactggaag
     8341 cattcccttt gaaaactggc acaagacagg gatgccctct ctcaccactc ctattcaaca
     8401 tagtgttgga agttctggcc agggcaatta ggcaggagaa ggaaataaag ggtattcaat
     8461 taggaaaaga ggaaatcaaa ttgtccctgt ttgcagatga catgattgta tatctagaaa
     8521 accccattgt ctaagcccaa aatctcctta agctgataag caacttcagc aaagtctcag
     8581 gatacaaaat caatgtacaa aaatcacaag aattcttata caccaataac agacaaacag
     8641 ccaaatcatg agtgaactcc cattcacaat tgcttcaaag agaataaaat acttaggaat
     8701 ccaacttaca agggacgtga aggacctctt caaggagaac tacaaaccac tgctcaatga
     8761 aataaaagag gatacaaaga aatggaagaa cattccatgc tcatgggtag gaagaatcaa
     8821 tatcgtgaaa atggccatac tgcccaaggt aatttataga ttcaatgcca tccccatcaa
     8881 gctaccaatg cctttcttca cagaattgca aaaaactact ttaaagttca tatggaacca
     8941 aaaaagagcc cgcatcgcca agtcaatcct aagccaaaag aacacagctg gaagcatcac
     9001 gctacctgac ttcaaactat actataaggc gacagtaacc aaaacagcat ggtaccagta
     9061 ccaaaacaga tatagatcaa tggaacagaa cagagccatc agaaataatg ccgcatatct
     9121 acaactatct gatctttgac aaacctgaga aaaacaagca atggggaaag gattccctat
     9181 ttaataaatg gtgctgggaa aactggctag ccatatgtag aaagctgaaa ctgtatccct
     9241 tccttacacc ttatacaaaa attaattcaa gatggattaa agacttaaac attagaccaa
     9301 aaaccataaa aaccctagaa gaaaacctag gcattaccat tcaggacata gggatgggca
     9361 aggacttcat gtctaaaaca ccaaaagcaa tggcaacaaa agccaaaatt gacaaatggg
     9421 atgtaattga actaaagagc ttctgcacag caaaagaaac taccatcaga gtgaacaggc
     9481 aacctacaaa atgggagaaa attttcacaa cctactcatc tgacaaaggg ctaatatcca
     9541 gaatctacaa tgaactcaaa caaatttaca agaaaaaaac aaccccatca aaaagtgggt
     9601 gaaggacatg aacagacact tctcaaaaga agacatttat gcagccaaaa aacacatgaa
     9661 aaaatgctca ccatcactgg ccatcagaca aatgcaaatc aaaaccacaa tgagatacca
     9721 tctcacacca gtcagaatgg caatcattaa aaagtcagga aacaacaggt gctggagagg
     9781 atgtggagaa ataggaacac ttttacactg ttggtgggac tgtaaactag ttcaagcatt
     9841 gtggaagtca gtttggcgat tcctcagtga tctagaacta gaaataccat ttgacccagc
     9901 catcccatta ctgggtatat acccaaagga atataaatca tgctgctata aagacacatg
     9961 cacacatatg tttattgcag cactattcac gatagcaaag acttggaatc aacccaaatg
    10021 tccaacaatg atagactgga ttaagaaaat gtggcacata tacaccatgg aatactatgc
    10081 agccataaaa aatgatgagt tcatgtcctt tgtagggaca tggatgaaat tggaaatcat
    10141 ccttctcagt aagctatcgc aagaacaaaa aaccaaatgc cgcatcttct cactcatagg
    10201 tgggaattga acaatgagaa cacatggaca caggaagggg aacatcacac tctggggact
    10261 gttgtggggt tgggggaggg gggagggata gctttaggag atatacctaa tgctaaatga
    10321 cgagttaatg ggtgcagcac accaacatgg cacatgtata catatgtaac taacctgcac
    10381 attgtgcaca tgtaccctga aacttaaagt ataataaaaa aaaaagaaat gtacttttaa
    10441 atttcagacc ttaaaaatat gccactaact tctaagttgt gatgtgatta catgagtttg
    10501 aaattcgttg atacttgctt tatgatctga tatgtgttgg ttttagtgaa atggcccatg
    10561 tgtgtaagaa aagaatgtgt tttgttaatt gattcatgtc ttttacatac tagtctgctt
    10621 aattcatcag tttctaactc gccagtttct aagagaggtg tgttaaaatc tcccaagatg
    10681 attggtggat tttgaatttt ccatatagtt ctgacgattt ttttgtgttt tatatgtttt
    10741 ttacattatt ttgtgcatag aaatttagaa ttacatcttt ttttttaagg ttcagatgag
    10801 aatttgtaca tttatgtgct aaaagtacag aacaatagtt gacattttca gagtacttgt
    10861 taagtgaatt ttaacaaaat agttgaaact tatttgaaaa tattttccct ttggtctttg
    10921 tggtagaaat gacagacttt ttcattgaat gatgaggaca cagcagccat attatgccaa
    10981 gaggaaaaat atcatgatca tattgaaaga tcaagcatga gcttccatgg tgaagtcttc
    11041 tgtaccaatg caccagagaa tgcctaccat ccaaaaaagc agtcagctga tggaaaagaa
    11101 gattattaaa cctttgttct gggatttact gttaaaaaac aaacaaaaaa caacaaaaaa
    11161 gcggtgacag gaaacagtta aggaaatgat ttaactgaag ggaagcaaga tttttttaaa
    11221 gcattgttct taatccccat gactgtcctt cagtcacaat tccagaataa aagaatctac
    11281 tttgtgta
//
