LOCUS       NM_002266               1977 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens karyopherin subunit alpha 2 (KPNA2), transcript
            variant 2, mRNA.
ACCESSION   NM_002266 XM_001133253 XM_001133254 XM_001133256 XM_001133258
            XM_001133262 XM_001133265 XM_001133267 XM_001133271
VERSION     NM_002266.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Suleman,M., Said,A., Khan,H., Rehman,S.U., Alshammari,A.,
            Crovella,S. and Yassine,H.M.
  TITLE     Mutational analysis of SARS-CoV-2 ORF6-KPNA2 binding interface and
            identification of potent small molecule inhibitors to recuse the
            host immune system
  JOURNAL   Front Immunol 14, 1266776 (2024)
   PUBMED   38283360
  REMARK    GeneRIF: Mutational analysis of SARS-CoV-2 ORF6-KPNA2 binding
            interface and identification of potent small molecule inhibitors to
            recuse the host immune system.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Lange,A., Mills,R.E., Lange,C.J., Stewart,M., Devine,S.E. and
            Corbett,A.H.
  TITLE     Classical nuclear localization signals: definition, function, and
            interaction with importin alpha
  JOURNAL   J Biol Chem 282 (8), 5101-5105 (2007)
   PUBMED   17170104
  REMARK    Review article
REFERENCE   3  (bases 1 to 1977)
  AUTHORS   Teng,S.C., Wu,K.J., Tseng,S.F., Wong,C.W. and Kao,L.
  TITLE     Importin KPNA2, NBS1, DNA repair and tumorigenesis
  JOURNAL   J Mol Histol 37 (5-7), 293-299 (2006)
   PUBMED   16752129
  REMARK    GeneRIF: This review focuses on recent experimental evidences
            demonstrating how NBS1 is translocated into the nucleus by an
            importin KPNA2 which mediates NBS1 subcellular localization and the
            functions of the NBS1 complex in tumorigenesis.
            Review article
REFERENCE   4  (bases 1 to 1977)
  AUTHORS   Dorr,S.N., Schlicker,M.N. and Hansmann,I.N.
  TITLE     Genomic structure of karyopherin alpha2 (KPNA2) within a low-copy
            repeat on chromosome 17q23-q24 and mutation analysis in patients
            with Russell-Silver syndrome
  JOURNAL   Hum Genet 109 (5), 479-486 (2001)
   PUBMED   11735022
REFERENCE   5  (bases 1 to 1977)
  AUTHORS   Herold,A., Truant,R., Wiegand,H. and Cullen,B.R.
  TITLE     Determination of the functional domain organization of the importin
            alpha nuclear import factor
  JOURNAL   J Cell Biol 143 (2), 309-318 (1998)
   PUBMED   9786944
REFERENCE   6  (bases 1 to 1977)
  AUTHORS   Weis,K., Ryder,U. and Lamond,A.I.
  TITLE     The conserved amino-terminal domain of hSRP1 alpha is essential for
            nuclear protein import
  JOURNAL   EMBO J 15 (8), 1818-1825 (1996)
   PUBMED   8617227
REFERENCE   7  (bases 1 to 1977)
  AUTHORS   Moroianu,J., Hijikata,M., Blobel,G. and Radu,A.
  TITLE     Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers:
            alpha 1 or alpha 2 subunit binds nuclear localization signal and
            beta subunit interacts with peptide repeat-containing nucleoporins
  JOURNAL   Proc Natl Acad Sci U S A 92 (14), 6532-6536 (1995)
   PUBMED   7604027
REFERENCE   8  (bases 1 to 1977)
  AUTHORS   Weis,K., Mattaj,I.W. and Lamond,A.I.
  TITLE     Identification of hSRP1 alpha as a functional receptor for nuclear
            localization sequences
  JOURNAL   Science 268 (5213), 1049-1053 (1995)
   PUBMED   7754385
REFERENCE   9  (bases 1 to 1977)
  AUTHORS   Cuomo,C.A., Kirch,S.A., Gyuris,J., Brent,R. and Oettinger,M.A.
  TITLE     Rch1, a protein that specifically interacts with the RAG-1
            recombination-activating protein
  JOURNAL   Proc Natl Acad Sci U S A 91 (13), 6156-6160 (1994)
   PUBMED   8016130
REFERENCE   10 (bases 1 to 1977)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U28386.1, AK024833.1 and
            AA744568.1.
            
            On Nov 23, 2018 this sequence version replaced NM_002266.3.
            
            Summary: The import of proteins into the nucleus is a process that
            involves at least 2 steps. The first is an energy-independent
            docking of the protein to the nuclear envelope and the second is an
            energy-dependent translocation through the nuclear pore complex.
            Imported proteins require a nuclear localization sequence (NLS)
            which generally consists of a short region of basic amino acids or
            2 such regions spaced about 10 amino acids apart. Proteins involved
            in the first step of nuclear import have been identified in
            different systems. These include the Xenopus protein importin and
            its yeast homolog, SRP1 (a suppressor of certain
            temperature-sensitive mutations of RNA polymerase I in
            Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein
            interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and
            may be involved in the nuclear transport of proteins. KPNA2 also
            may play a role in V(D)J recombination. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Feb
            2016].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053343.1, AK291954.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330459.8/ ENSP00000332455.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-929               U28386.1           4-932
            930-1965            AK024833.1         931-1966
            1966-1977           AA744568.1         17-28               c
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q24.2"
     gene            1..1977
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /note="karyopherin subunit alpha 2"
                     /db_xref="GeneID:3838"
                     /db_xref="HGNC:HGNC:6395"
                     /db_xref="MIM:600685"
     exon            1..106
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            107..204
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1719
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /note="importin-alpha-P1; pendulin; importin subunit
                     alpha-2; RAG cohort protein 1; karyopherin alpha 2 (RAG
                     cohort 1, importin alpha 1); nuclear pore-targeting
                     complex 58kD component"
                     /codon_start=1
                     /product="importin subunit alpha-1"
                     /protein_id="NP_002257.1"
                     /db_xref="CCDS:CCDS32713.1"
                     /db_xref="GeneID:3838"
                     /db_xref="HGNC:HGNC:6395"
                     /db_xref="MIM:600685"
                     /translation="MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKD
                     DQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARK
                     LLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVD
                     GGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDM
                     SSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAIS
                     YLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDA
                     GALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFK
                     TQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQA
                     AEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVP
                     ETTSEGYTFQVQDGAPGTFNF"
     exon            205..342
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            343..431
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            432..700
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            701..795
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            796..1059
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1293
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1476
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1626
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1977
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1735..1740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /note="hexamer: CATAAA"
     polyA_site      1754
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /note="major polyA site"
     regulatory      1943..1948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
                     /note="hexamer: AATAAA"
     polyA_site      1977
                     /gene="KPNA2"
                     /gene_synonym="IPOA1; PTAC58; QIP2; RCH1; SRP1-alpha;
                     SRP1alpha"
ORIGIN      
        1 acacggtctt tgagctgagt cgaggtggac cctttgaacg cagtcgccct acagccgctg
       61 attccccccg catcgcctcc cgtggaagcc caggcccgct tcgcagcttt ctccctttgt
      121 ctcataacca tgtccaccaa cgagaatgct aatacaccag ctgcccgtct tcacagattc
      181 aagaacaagg gaaaagacag tacagaaatg aggcgtcgca gaatagaggt caatgtggag
      241 ctgaggaaag ctaagaagga tgaccagatg ctgaagagga gaaatgtaag ctcatttcct
      301 gatgatgcta cttctccgct gcaggaaaac cgcaacaacc agggcactgt aaattggtct
      361 gttgatgaca ttgtcaaagg cataaatagc agcaatgtgg aaaatcagct ccaagctact
      421 caagctgcca ggaaactact ttccagagaa aaacagcccc ccatagacaa cataatccgg
      481 gctggtttga ttccgaaatt tgtgtccttc ttgggcagaa ctgattgtag tcccattcag
      541 tttgaatctg cttgggcact cactaacatt gcttctggga catcagaaca aaccaaggct
      601 gtggtagatg gaggtgccat cccagcattc atttctctgt tggcatctcc ccatgctcac
      661 atcagtgaac aagctgtctg ggctctagga aacattgcag gtgatggctc agtgttccga
      721 gacttggtta ttaagtacgg tgcagttgac ccactgttgg ctctccttgc agttcctgat
      781 atgtcatctt tagcatgtgg ctacttacgt aatcttacct ggacactttc taatctttgc
      841 cgcaacaaga atcctgcacc cccgatagat gctgttgagc agattcttcc taccttagtt
      901 cggctcctgc atcatgatga tccagaagta ttagcagata cctgctgggc tatttcctac
      961 cttactgatg gtccaaatga acgaattggc atggtggtga aaacaggagt tgtgccccaa
     1021 cttgtgaagc ttctaggagc ttctgaattg ccaattgtga ctcctgccct aagagccata
     1081 gggaatattg tcactggtac agatgaacag actcaggttg tgattgatgc aggagcactc
     1141 gccgtctttc ccagcctgct caccaacccc aaaactaaca ttcagaagga agctacgtgg
     1201 acaatgtcaa acatcacagc cggccgccag gaccagatac agcaagttgt gaatcatgga
     1261 ttagtcccat tccttgtcag tgttctctct aaggcagatt ttaagacaca aaaggaagct
     1321 gtgtgggccg tgaccaacta taccagtggt ggaacagttg aacagattgt gtaccttgtt
     1381 cactgtggca taatagaacc gttgatgaac ctcttaactg caaaagatac caagattatt
     1441 ctggttatcc tggatgccat ttcaaatatc tttcaggctg ctgagaaact aggtgaaact
     1501 gagaaactta gtataatgat tgaagaatgt ggaggcttag acaaaattga agctctacaa
     1561 aaccatgaaa atgagtctgt gtataaggct tcgttaagct taattgagaa gtatttctct
     1621 gtagaggaag aggaagatca aaacgttgta ccagaaacta cctctgaagg ctacactttc
     1681 caagttcagg atggggctcc tgggaccttt aacttttaga tcatgtagct gagacataaa
     1741 tttgttgtgt actacgtttg gtattttgtc ttattgtttc tctactaaga actctttctt
     1801 aaatgtggtt tgttactgta gcacttttta cactgaaact atacttgaac agttccaact
     1861 gtacatacat actgtatgaa gcttgtcctc tgactaggtt tctaatttct atgtggaatt
     1921 tcctatcttg cagcatcctg taaataaaca ttcaagtcca cccttttctt gacttca
//
