LOCUS       NM_002264               6888 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens karyopherin subunit alpha 1 (KPNA1), transcript
            variant 1, mRNA.
ACCESSION   NM_002264
VERSION     NM_002264.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Zhao,L., Wu,D., Qu,Q., Li,Z. and Yin,H.
  TITLE     Karyopherin Subunit Alpha 1 Enhances the Malignant Behaviors of
            Colon Cancer Cells via Promoting Nuclear Factor-kappaB p65 Nuclear
            Translocation
  JOURNAL   Dig Dis Sci 68 (7), 3018-3031 (2023)
   PUBMED   37038032
  REMARK    GeneRIF: Karyopherin Subunit Alpha 1 Enhances the Malignant
            Behaviors of Colon Cancer Cells via Promoting Nuclear Factor-kappaB
            p65 Nuclear Translocation.
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Jiang,L., Li,D., Wang,C., Liao,J., Liu,J., Wei,Q. and Wang,Y.
  TITLE     Decreased Expression of Karyopherin-alpha 1 is Related to the
            Malignant Degree of Cervical Cancer and is Critical for the
            Proliferation of Hela Cells
  JOURNAL   Pathol Oncol Res 28, 1610518 (2022)
   PUBMED   35991835
  REMARK    GeneRIF: Decreased Expression of Karyopherin-alpha 1 is Related to
            the Malignant Degree of Cervical Cancer and is Critical for the
            Proliferation of Hela Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6888)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 6888)
  AUTHORS   Tsoi,H., Man,E.P., Leung,M.H., Mok,K.C., Chau,K.M., Wong,L.S.,
            Chan,W.L., Chan,S.Y., Luk,M.Y., Cheng,C.N. and Khoo,U.S.
  TITLE     KPNA1 regulates nuclear import of NCOR2 splice variant BQ323636.1
            to confer tamoxifen resistance in breast cancer
  JOURNAL   Clin Transl Med 11 (10), e554 (2021)
   PUBMED   34709749
  REMARK    GeneRIF: KPNA1 regulates nuclear import of NCOR2 splice variant
            BQ323636.1 to confer tamoxifen resistance in breast cancer.
REFERENCE   5  (bases 1 to 6888)
  AUTHORS   de Oliveira,H.C., da Silva,T.D., Salvador,G.H.M., Moraes,I.R.,
            Fukuda,C.A., de Barros,A.C. and Fontes,M.R.M.
  TITLE     Structural and calorimetric studies reveal specific determinants
            for the binding of a high-affinity NLS to mammalian importin-alpha
  JOURNAL   Biochem J 478 (13), 2715-2732 (2021)
   PUBMED   34195786
  REMARK    GeneRIF: Structural and calorimetric studies reveal specific
            determinants for the binding of a high-affinity NLS to mammalian
            importin-alpha.
REFERENCE   6  (bases 1 to 6888)
  AUTHORS   Di Marzio,P., Choe,S., Ebright,M., Knoblauch,R. and Landau,N.R.
  TITLE     Mutational analysis of cell cycle arrest, nuclear localization and
            virion packaging of human immunodeficiency virus type 1 Vpr
  JOURNAL   J Virol 69 (12), 7909-7916 (1995)
   PUBMED   7494303
REFERENCE   7  (bases 1 to 6888)
  AUTHORS   O'Neill,R.E., Jaskunas,R., Blobel,G., Palese,P. and Moroianu,J.
  TITLE     Nuclear import of influenza virus RNA can be mediated by viral
            nucleoprotein and transport factors required for protein import
  JOURNAL   J Biol Chem 270 (39), 22701-22704 (1995)
   PUBMED   7559393
REFERENCE   8  (bases 1 to 6888)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
REFERENCE   9  (bases 1 to 6888)
  AUTHORS   Sharova,N. and Bukrinskaya,A.
  TITLE     p17 and p17-containing gag precursors of input human
            immunodeficiency virus are transported into the nuclei of infected
            cells
  JOURNAL   AIDS Res Hum Retroviruses 7 (3), 303-306 (1991)
   PUBMED   2064827
REFERENCE   10 (bases 1 to 6888)
  AUTHORS   Mukaigawa,J. and Nayak,D.P.
  TITLE     Two signals mediate nuclear localization of influenza virus
            (A/WSN/33) polymerase basic protein 2
  JOURNAL   J Virol 65 (1), 245-253 (1991)
   PUBMED   1985200
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA308391.1, U20620.1,
            BC003009.1 and AC083798.14.
            
            On Nov 22, 2018 this sequence version replaced NM_002264.3.
            
            Summary: The transport of molecules between the nucleus and the
            cytoplasm in eukaryotic cells is mediated by the nuclear pore
            complex (NPC), which consists of 60-100 proteins. Small molecules
            (up to 70 kD) can pass through the nuclear pore by nonselective
            diffusion while larger molecules are transported by an active
            process. The protein encoded by this gene belongs to the importin
            alpha family, and is involved in nuclear protein import. This
            protein interacts with the recombination activating gene 1 (RAG1)
            protein and is a putative substrate of the RAG1 ubiquitin ligase.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) lacks an alternate exon
            compared to variant 2 and represents the protein-coding transcript.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.80941.1,
                                           SRR1803616.269053.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344337.11/ ENSP00000343701.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-405               DA308391.1         1-405
            406-737             U20620.1           71-402
            738-1826            BC003009.1         727-1815
            1827-3111           U20620.1           1491-2775
            3112-6888           AC083798.14        136721-140497       c
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.1"
     gene            1..6888
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="karyopherin subunit alpha 1"
                     /db_xref="GeneID:3836"
                     /db_xref="HGNC:HGNC:6394"
                     /db_xref="MIM:600686"
     exon            1..183
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            184..317
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..1805
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="importin-alpha-S1; importin alpha 5; importin
                     subunit alpha-1; recombination activating gene cohort 2;
                     nucleoprotein interactor 1; SRP1-beta; RAG cohort protein
                     2; karyopherin alpha 1 (importin alpha 5)"
                     /codon_start=1
                     /product="importin subunit alpha-5"
                     /protein_id="NP_002255.3"
                     /db_xref="CCDS:CCDS3013.1"
                     /db_xref="GeneID:3836"
                     /db_xref="HGNC:HGNC:6394"
                     /db_xref="MIM:600686"
                     /translation="MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQL
                     FKRRNVATAEEETEEEVMSDGGFHEAQISNMEMAPGGVITSDMIEMIFSKSPEQQLSA
                     TQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSL
                     QTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQ
                     LFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACW
                     ALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVI
                     LNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTA
                     EFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENI
                     LRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTED
                     EDSSIAPQVDLNQQQYIFQQCEAPMEGFQL"
     exon            318..425
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            426..525
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..620
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..752
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            753..841
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..941
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1105
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1184
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1310
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1438
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1617
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..6888
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3079..3084
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="hexamer: AATAAA"
     polyA_site      3112
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="major polyA site"
     polyA_site      5203
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
     regulatory      6635..6640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="hexamer: ATTAAA"
     polyA_site      6656
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
     regulatory      6819..6824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="hexamer: AATAAA"
     polyA_site      6839
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
     regulatory      6863..6868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /note="hexamer: AGTAAA"
     polyA_site      6888
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
ORIGIN      
        1 gcactgcgaa cgccggctga gctgagtctc gctgctcggt gcgaggcggc ggagagcgag
       61 gcctggtgag caccgccgag gcgcgggcca gctcttcgag gttgtgcgcg ggagtggcac
      121 ggcgggcggg cgagcgaggg gctaacttca gcggtggcac cgggatcggt tgccttgagc
      181 ctgaaatcat gaccacccca ggaaaagaga actttcgcct gaaaagttac aagaacaaat
      241 ctctgaatcc cgatgagatg cgcaggagga gggaggaaga aggactgcag ttacgaaagc
      301 agaaaagaga agagcagtta ttcaagcgga gaaatgttgc tacagcagaa gaagaaacag
      361 aagaagaagt tatgtcagat ggaggctttc atgaggctca gattagtaac atggagatgg
      421 caccaggtgg tgtcatcact tctgacatga ttgaaatgat attttccaaa agcccagagc
      481 aacagctttc agcaacacag aaattcagga agctgctttc aaaagaacct aaccctccta
      541 ttgatgaagt tatcagcaca ccaggagtag tggccaggtt tgtggagttc ctcaaacgaa
      601 aagagaattg tacactgcag tttgaatcag cttgggtact gacaaatatt gcttcaggaa
      661 attctcttca gacccgaatt gtgattcagg caggagctgt gcccatcttc atagagttgc
      721 tcagctcaga gtttgaagat gtccaggaac aggcagtctg ggctcttggc aacattgctg
      781 gagatagtac catgtgcagg gactatgtct tagactgcaa tatccttccc cctcttttgc
      841 agttattttc aaagcaaaac cgcctgacca tgacccggaa tgcagtatgg gctttgtcta
      901 atctctgtag agggaaaagt ccacctccag aatttgcaaa ggtttctcca tgtctgaatg
      961 tgctttcctg gttgctgttt gtcagtgaca ctgatgtact ggctgatgcc tgctgggccc
     1021 tctcatatct atcagatgga cccaatgata aaattcaagc ggtcatcgat gcgggagtat
     1081 gtaggagact tgtggaactg ctgatgcata atgattataa agtggtttct cctgctttgc
     1141 gagctgtggg aaacattgtc acaggggatg atattcagac acaggtaatt ctgaattgct
     1201 cagctctgca gagtttattg catttgctga gtagcccaaa ggaatctatc aaaaaggaag
     1261 catgttggac gatatctaat attacagctg gaaatagggc acagatccag actgtgatag
     1321 atgccaacat tttcccagcc ctcattagta ttttacaaac tgctgaattt cggacaagaa
     1381 aagaagcagc ttgggccatc acaaatgcaa cttctggagg atcagctgaa cagatcaagt
     1441 acctagtaga actgggttgt atcaagccgc tctgtgatct cctcacggtc atggactcta
     1501 agattgtaca ggttgcccta aatggcttgg aaaatatcct gaggcttgga gaacaggaag
     1561 ccaaaaggaa tggcactggc attaaccctt actgtgcttt gattgaagaa gcttatggtc
     1621 tggataaaat tgagttctta cagagtcatg aaaaccagga gatctaccaa aaggcctttg
     1681 atcttattga gcattacttc gggaccgaag atgaagacag cagcattgca ccccaggttg
     1741 accttaacca gcagcagtac atcttccaac agtgtgaggc tcctatggaa ggtttccagc
     1801 tttgaagcaa tactctgctt tcacgtacct gtgtcagacc aggctaccca gtcgagtcct
     1861 cttgtggagc ccacagtcct catggagcta acttctcaaa tgttttccat aatactgttt
     1921 gcgctcattt gcttgccttg cgcacctgct ctcttacaca catctggaaa acctccggct
     1981 ctctgtggtg gaataccctt ctaataaaag ggtaaccaga acggcccact ctcttttacg
     2041 gaaaaatccc taggctttgg agatccgcac ttacattaga gttatgggaa tatacacata
     2101 ttaatgtggc tccctttttc ttgtggggga ataaaagagg actcctcctc attcccttta
     2161 acatggggga aaaaactgac attaaaagat gagactaaat ctttatcttg aattttacac
     2221 aactacttac gacaagggag atgtttagac ctgttgtgta tacttcagag tacttttcat
     2281 gagttcttcc acagtgaacc cttggattac ctggtggctt tttctagcca gatttgcatt
     2341 aatccttact gagattggat ggttttcttt cctctattgg cgccattctt cagatattaa
     2401 agttaaacca tccactccct caccttcagc cttcagtgaa tgtgctttct agttgtcagg
     2461 aatgctgaag aattaacact ttgactccta aatgtgatac tggtttgtag attcccttag
     2521 agcagaaagg agaggggcac atattaattt gtatcgcttt tgcttctctt tggtcttttg
     2581 tgtcttagaa tttggaagtg gttcatttct gttgctggta tgaggatttc gaatacttag
     2641 taatcgaaaa ccatatcctg taatttaata aaaaaaacta aggacgaaaa aaccctccaa
     2701 ttttcccaaa tgcaatcagt gtaactaggg gctgtgtttc tgcattaaaa taaatgtttc
     2761 aggctttgtg gtcctgatca aggtcctcat taaaaaattg gagttcaccc taggtgcttt
     2821 tcccctctgt gactggcaga taacacatac ttttgaaagt aactttggga tttttttctt
     2881 aggtgcagct cgattctaat cttttcatgc tgcacacgat tcctttaatg tagcatcctt
     2941 atctgaaaga aataaccatc ttctcaacat gacctgctta acccaaataa gaacagtgat
     3001 cttataacct cattgtttcc taatcatttt atttcatctc ctgctagtac tgtgccgctt
     3061 ccccctcccc ccacacaaaa taaaaacagt atctgccttc tggctcattt aatgatgact
     3121 gcaatctgta cctgcaaatg gcgcttccag tactgtgttc agtgatccac atttgtgctt
     3181 ggactggaat gaaaaaatac gcttaattgc caagacaact gcaaatgagt tcaatacgac
     3241 ttctcggtgc tagttggcca tcttgactca atgttgggat acactaccag agaagatttt
     3301 tttgttgatg tgctgttcaa agtgaattct gaaggaaaag gttgctcacc tctgtttcag
     3361 agcataccct tttaaacctt tttagacttg tcctcttcta gaattcatta taaccccaaa
     3421 gtgtaaaaac tatttggaag ttgcccctga caagctatta tacatctctg gtaaatcctg
     3481 ctgaatgtaa gggatattca gagcttttca tacctcatca tgatgttatt taagcagcca
     3541 acttatgtga cctgatttaa acttttaaaa aatccacttc attcaaacta gaaagagtca
     3601 aagacaaagg acatttatca gccaagagtg tgacgcattc ttcgcatcta gtctgtagta
     3661 gtgtctgtgc tctgggatgg ataccgtctg atgtctgttt gttattgatg gcgcagccac
     3721 tgcaaaacta gtcaactccc atctataact gttacttttt ctagtgctgc tttgtgctga
     3781 gagaacattt tagtttactg cacttgacct gtaaaagact gattctttgt aattttaggg
     3841 ataaattagg tggttaattt aaagaagaaa tttcaatgtt gcctttacat cacattaaaa
     3901 tataacttct ttcagaagga taatagaagc actgattcat agtaaaaatc ttgtttttag
     3961 ctttgacttt tttgtggctg ttttttaaaa ttgtaaatta ctagtaacat gtcatttcta
     4021 taggatgttt taaattatgt accttccatt ggatagttaa aataatttca ttttataagc
     4081 caatttgagt gggaaagata caaagcacac agttgtctcc ccacattagg aagagtagtg
     4141 tgcattcaga cattcggaat ttttgctagt agttggagat gtttcagtga gtgaattaaa
     4201 acaaagtcta tttaactgac gtgatctatt ttgtgtctaa aacagaggaa tttatcttgt
     4261 cctagaagtt catgaactat aagatctaag tttttttttc ttttttaaca ataaaatgat
     4321 tttttcttaa agtatcaaag ttggaattgg ctaaaagtga agtagcataa ctcagatatg
     4381 ttggaacttg gtactgaagc aggctttttg gtaacagaaa tgacatttta aggggtaact
     4441 gtatatcctg tttttagttc tttgcctctc agattctgtt aacttagttt aaattcactg
     4501 aataccctac cactaaggcg ttgctcatta caaaagcatg ttaatttcaa tacaggaaaa
     4561 ctcttttgta gttttatata tggattgggg aaagtgactt tgttttactt ttccttttta
     4621 ttcccctcca tcctccaaac ttccaatcta tattatttgt aatgagaact ttggtggggg
     4681 aaaaaaagca ttaagagaat tttggatgta aaggcatctg gtatctgatg gaccagcagg
     4741 tagatgtggt tggattgaga aattttgggt aaggtagatg atagaagaaa gagattttag
     4801 gagacacagt tctctgtgga acactttctt tcatgttcct gtaactaatt gatcaagacc
     4861 cttttaaacg cttatctttg tcagttccgc ttttgaaaaa actattgccc taggattgtt
     4921 tcttccatat gacagatgaa ggttattggc tctgagtcat ttttggcatt atcgtcacag
     4981 ataaaaagat tggcaattaa tctcataaaa aatctgaatt attccagatt gctgccgatt
     5041 tttgcctaag aaacaatatg taagtatatt ttataaacca aactttgtca accaattcta
     5101 ttgtacgtct acccacaggc aactttgaat ttacttctct agtgctaaat aaacatttca
     5161 gcgatttaat cccccaactt gggggatgca ttatgaaaag gttttcctgg agtcaagggg
     5221 tttttctttc tcgctagttt tgtgaaaatt tttatatcaa taggcaaatc attataactt
     5281 tttaggggtt gatggttttt aagtatattg gaaattgtgg tgtctaagga tgatgttcag
     5341 tgaagaaaag ggatccactt ttagaaacca gcattgtttg ctttcaactt tcatactgta
     5401 tgctaactcc tacgttccaa aatgtgctca tttgcagaca caggtatgcc tatatacatg
     5461 gaagtctcaa atctgaattt ttatccatct caatatgacc atttctctct gttgtgagct
     5521 gaacagatta agtatatatc tgccaggttg ggaaatattt tggtctatct tttcctgtca
     5581 tcagaactta atttaaaaaa attatcaaag gtcagatgtg actactacag taagttggct
     5641 atcataaaga atattccata aaatgtttta tctgccatac aaaattactg ggtttatggc
     5701 cggatgtggt ggctcatgcc tgtaatccca gcagttcagg attacaggtt atatacaggt
     5761 tataacaatg gataccagga catcagaata tctgataaag caaatattta tatgctaatt
     5821 taaaatatca aattgctact ggacataaaa tacatctgga agcttggggt aagaagaaag
     5881 aaaagaagtg ttccgttctg ttttcaacta agggtaaacg aagtcccaga gtgttttccc
     5941 tgtaggtcaa attaaggtaa catgtcttta tttgatcatc tattgtacac cagatacctg
     6001 gctaagggct ttcctttttt ctcatgtagt cttccaaatg ttctttgata attgtcacta
     6061 tattatagat gacaaagtga agacttacga gaaattactt tgccccaagg ttacaacact
     6121 tagatggctg tccaagggcg gggaagagcc ctgcaaattc tgtgtcttga agaaagcagt
     6181 ggccacgcag tagttccgtg cccaggatct cccagaacta gacttggcaa ccacagctgg
     6241 gccagccttt tttgttagac cattcttcct gactcctgct taccatcagc acccttaaaa
     6301 tgacgaaatc aggcttaagt tgcttttagg ttacacagac tggagtgtag gccctctcag
     6361 gccaaacatc tttcctcttc tgtggtgcta aaattctgta tgaccagaca agtatggtaa
     6421 taggtttttt tttttttttt ttttgtgcaa atgattttgt gtttagtaaa ataatggcta
     6481 aacacatgga tccattcaaa cgcacaaatg tggaaagtat tgacacggaa ggaaaggctt
     6541 tgaagaatta ctgcctcagg aagcagtgcc atggatgcga atttaacaca gtctacttct
     6601 tcctaaagga atatcatccc aaggaatgtc aaaaattaaa atcacctttt aatgtactgg
     6661 ttgttgagtg tttctgcttt acctcaagtc attctgacca ctctacatcc tgcttcaaag
     6721 tgttctgaca cttagatttg gaatggctct aatgattctt tgtacttgaa tgtggtctgt
     6781 caggccatgg gtgagtgcct tgcacatagt agacattcaa taaatactaa atgaaggaat
     6841 atgcatgcat ctttgtgatg ttagtaaagg gtgcttgata aaagaaaa
//
