LOCUS       NP_002255                538 aa            linear   PRI 19-SEP-2024
DEFINITION  importin subunit alpha-5 [Homo sapiens].
ACCESSION   NP_002255
VERSION     NP_002255.3
DBSOURCE    REFSEQ: accession NM_002264.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 538)
  AUTHORS   Zhao,L., Wu,D., Qu,Q., Li,Z. and Yin,H.
  TITLE     Karyopherin Subunit Alpha 1 Enhances the Malignant Behaviors of
            Colon Cancer Cells via Promoting Nuclear Factor-kappaB p65 Nuclear
            Translocation
  JOURNAL   Dig Dis Sci 68 (7), 3018-3031 (2023)
   PUBMED   37038032
  REMARK    GeneRIF: Karyopherin Subunit Alpha 1 Enhances the Malignant
            Behaviors of Colon Cancer Cells via Promoting Nuclear Factor-kappaB
            p65 Nuclear Translocation.
REFERENCE   2  (residues 1 to 538)
  AUTHORS   Jiang,L., Li,D., Wang,C., Liao,J., Liu,J., Wei,Q. and Wang,Y.
  TITLE     Decreased Expression of Karyopherin-alpha 1 is Related to the
            Malignant Degree of Cervical Cancer and is Critical for the
            Proliferation of Hela Cells
  JOURNAL   Pathol Oncol Res 28, 1610518 (2022)
   PUBMED   35991835
  REMARK    GeneRIF: Decreased Expression of Karyopherin-alpha 1 is Related to
            the Malignant Degree of Cervical Cancer and is Critical for the
            Proliferation of Hela Cells.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 538)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (residues 1 to 538)
  AUTHORS   Tsoi,H., Man,E.P., Leung,M.H., Mok,K.C., Chau,K.M., Wong,L.S.,
            Chan,W.L., Chan,S.Y., Luk,M.Y., Cheng,C.N. and Khoo,U.S.
  TITLE     KPNA1 regulates nuclear import of NCOR2 splice variant BQ323636.1
            to confer tamoxifen resistance in breast cancer
  JOURNAL   Clin Transl Med 11 (10), e554 (2021)
   PUBMED   34709749
  REMARK    GeneRIF: KPNA1 regulates nuclear import of NCOR2 splice variant
            BQ323636.1 to confer tamoxifen resistance in breast cancer.
REFERENCE   5  (residues 1 to 538)
  AUTHORS   de Oliveira,H.C., da Silva,T.D., Salvador,G.H.M., Moraes,I.R.,
            Fukuda,C.A., de Barros,A.C. and Fontes,M.R.M.
  TITLE     Structural and calorimetric studies reveal specific determinants
            for the binding of a high-affinity NLS to mammalian importin-alpha
  JOURNAL   Biochem J 478 (13), 2715-2732 (2021)
   PUBMED   34195786
  REMARK    GeneRIF: Structural and calorimetric studies reveal specific
            determinants for the binding of a high-affinity NLS to mammalian
            importin-alpha.
REFERENCE   6  (residues 1 to 538)
  AUTHORS   Di Marzio,P., Choe,S., Ebright,M., Knoblauch,R. and Landau,N.R.
  TITLE     Mutational analysis of cell cycle arrest, nuclear localization and
            virion packaging of human immunodeficiency virus type 1 Vpr
  JOURNAL   J Virol 69 (12), 7909-7916 (1995)
   PUBMED   7494303
REFERENCE   7  (residues 1 to 538)
  AUTHORS   O'Neill,R.E., Jaskunas,R., Blobel,G., Palese,P. and Moroianu,J.
  TITLE     Nuclear import of influenza virus RNA can be mediated by viral
            nucleoprotein and transport factors required for protein import
  JOURNAL   J Biol Chem 270 (39), 22701-22704 (1995)
   PUBMED   7559393
REFERENCE   8  (residues 1 to 538)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
REFERENCE   9  (residues 1 to 538)
  AUTHORS   Sharova,N. and Bukrinskaya,A.
  TITLE     p17 and p17-containing gag precursors of input human
            immunodeficiency virus are transported into the nuclei of infected
            cells
  JOURNAL   AIDS Res Hum Retroviruses 7 (3), 303-306 (1991)
   PUBMED   2064827
REFERENCE   10 (residues 1 to 538)
  AUTHORS   Mukaigawa,J. and Nayak,D.P.
  TITLE     Two signals mediate nuclear localization of influenza virus
            (A/WSN/33) polymerase basic protein 2
  JOURNAL   J Virol 65 (1), 245-253 (1991)
   PUBMED   1985200
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA308391.1, U20620.1,
            BC003009.1 and AC083798.14.
            
            On Jan 30, 2009 this sequence version replaced NP_002255.2.
            
            Summary: The transport of molecules between the nucleus and the
            cytoplasm in eukaryotic cells is mediated by the nuclear pore
            complex (NPC), which consists of 60-100 proteins. Small molecules
            (up to 70 kD) can pass through the nuclear pore by nonselective
            diffusion while larger molecules are transported by an active
            process. The protein encoded by this gene belongs to the importin
            alpha family, and is involved in nuclear protein import. This
            protein interacts with the recombination activating gene 1 (RAG1)
            protein and is a putative substrate of the RAG1 ubiquitin ligase.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) lacks an alternate exon
            compared to variant 2 and represents the protein-coding transcript.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.80941.1,
                                           SRR1803616.269053.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344337.11/ ENSP00000343701.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.1"
     Protein         1..538
                     /product="importin subunit alpha-5"
                     /note="importin-alpha-S1; importin alpha 5; importin
                     subunit alpha-1; recombination activating gene cohort 2;
                     nucleoprotein interactor 1; SRP1-beta; RAG cohort protein
                     2; karyopherin alpha 1 (importin alpha 5)"
                     /calculated_mol_wt=60091
     Region          1..36
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0007744|PubMed:22814378; propagated from
                     UniProtKB/Swiss-Prot (P52294.3)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylthreonine, in Importin subunit alpha-5,
                     N-terminally processed.
                     /evidence=ECO:0007744|PubMed:22814378; propagated from
                     UniProtKB/Swiss-Prot (P52294.3)"
     Site            3
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (P52294.3)"
     Region          33..516
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          42..51
                     /region_name="nuclear localization signal"
     Site            63
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (P52294.3)"
     Region          77..117
                     /region_name="ARM 1, truncated"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          118..161
                     /region_name="ARM 2"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          128..154
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(145,149,153,187,191,195,230,234,238)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          149..241
                     /region_name="NLS binding site (major).
                     /evidence=ECO:0000250|UniProtKB:P52293"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          162..198
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          204..239
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          207..245
                     /region_name="ARM 4"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          245..437
                     /region_name="Binding to RAG1.
                     /evidence=ECO:0000269|PubMed:8052633"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          256..281
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          289..325
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          318..406
                     /region_name="NLS binding site (minor).
                     /evidence=ECO:0000250|UniProtKB:P52293"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Site            order(356,360,364,398,402,406,441,445,449)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          373..409
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          413..457
                     /region_name="ARM 9"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     Region          416..450
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          460..504
                     /region_name="ARM 10, atypical"
                     /note="propagated from UniProtKB/Swiss-Prot (P52294.3)"
     CDS             1..538
                     /gene="KPNA1"
                     /gene_synonym="IPOA5; NPI-1; RCH2; SRP1"
                     /coded_by="NM_002264.4:189..1805"
                     /db_xref="CCDS:CCDS3013.1"
                     /db_xref="GeneID:3836"
                     /db_xref="HGNC:HGNC:6394"
                     /db_xref="MIM:600686"
ORIGIN      
        1 mttpgkenfr lksyknksln pdemrrrree eglqlrkqkr eeqlfkrrnv ataeeeteee
       61 vmsdggfhea qisnmemapg gvitsdmiem ifskspeqql satqkfrkll skepnppide
      121 vistpgvvar fveflkrken ctlqfesawv ltniasgnsl qtriviqaga vpifiellss
      181 efedvqeqav walgniagds tmcrdyvldc nilppllqlf skqnrltmtr navwalsnlc
      241 rgkspppefa kvspclnvls wllfvsdtdv ladacwalsy lsdgpndkiq avidagvcrr
      301 lvellmhndy kvvspalrav gnivtgddiq tqvilncsal qsllhllssp kesikkeacw
      361 tisnitagnr aqiqtvidan ifpalisilq taefrtrkea awaitnatsg gsaeqikylv
      421 elgcikplcd lltvmdskiv qvalngleni lrlgeqeakr ngtginpyca lieeaygldk
      481 ieflqshenq eiyqkafdli ehyfgteded ssiapqvdln qqqyifqqce apmegfql
//
