LOCUS       NM_033286               1757 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens kinetochore localized astrin (SPAG5) binding protein
            (KNSTRN), transcript variant 1, mRNA.
ACCESSION   NM_033286 XM_031561
VERSION     NM_033286.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Qin,B., Zhu,Z., Yin,F., Yan,D., Wan,J., Dong,M. and Wang,Z.
  TITLE     Phosphorylation of small kinetochore-associated protein induced by
            GSK3beta promotes cell migration and invasion in esophageal cancer
  JOURNAL   Cell Cycle 21 (9), 972-983 (2022)
   PUBMED   35201967
  REMARK    GeneRIF: Phosphorylation of small kinetochore-associated protein
            induced by GSK3beta promotes cell migration and invasion in
            esophageal cancer.
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1757)
  AUTHORS   Zhang,M., Yang,F., Wang,W., Zohbi,N., Wang,X., Wang,D., Zhuang,X.,
            Dou,Z., Liu,D., Song,X., Green,H.N., Liu,X. and Yao,X.
  TITLE     SKAP interacts with Aurora B to guide end-on capture of spindle
            microtubules via phase separation
  JOURNAL   J Mol Cell Biol 13 (12), 841-852 (2022)
   PUBMED   34554241
  REMARK    GeneRIF: SKAP interacts with Aurora B to guide end-on capture of
            spindle microtubules via phase separation.
REFERENCE   4  (bases 1 to 1757)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1757)
  AUTHORS   Morii,M., Kubota,S., Hasegawa,C., Takeda,Y., Kometani,S.,
            Enomoto,K., Suzuki,T., Yanase,S., Sato,R., Akatsu,A., Hirata,K.,
            Honda,T., Kuga,T., Tomonaga,T., Nakayama,Y., Yamaguchi,N. and
            Yamaguchi,N.
  TITLE     Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on
            the mitotic spindle
  JOURNAL   Sci Rep 11 (1), 2616 (2021)
   PUBMED   33510346
  REMARK    GeneRIF: Src-mediated tyrosine phosphorylation of PRC1 and
            kinastrin/SKAP on the mitotic spindle.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1757)
  AUTHORS   Dunsch,A.K., Linnane,E., Barr,F.A. and Gruneberg,U.
  TITLE     The astrin-kinastrin/SKAP complex localizes to microtubule plus
            ends and facilitates chromosome alignment
  JOURNAL   J Cell Biol 192 (6), 959-968 (2011)
   PUBMED   21402792
REFERENCE   7  (bases 1 to 1757)
  AUTHORS   Roifman,C.M. and Chitayat,D.
  TITLE     Combined immunodeficiency, facial dysmorphism, optic nerve atrophy,
            skeletal anomalies and developmental delay: a new syndrome
  JOURNAL   Clin Genet 76 (5), 449-457 (2009)
   PUBMED   19863561
REFERENCE   8  (bases 1 to 1757)
  AUTHORS   Fang,L., Seki,A. and Fang,G.
  TITLE     SKAP associates with kinetochores and promotes the
            metaphase-to-anaphase transition
  JOURNAL   Cell Cycle 8 (17), 2819-2827 (2009)
   PUBMED   19667759
  REMARK    GeneRIF: This paper identifies role of gene product of FLJ14502,
            named SKAP in the paper, in mitosis. SKAP promotes
            metaphase-to-anaphase transition by mediating seperase activation
            after spindle assembly checkpoint is satisfied in mitosis.
REFERENCE   9  (bases 1 to 1757)
  AUTHORS   Tsang,H.T., Connell,J.W., Brown,S.E., Thompson,A., Reid,E. and
            Sanderson,C.M.
  TITLE     A systematic analysis of human CHMP protein interactions:
            additional MIT domain-containing proteins bind to multiple
            components of the human ESCRT III complex
  JOURNAL   Genomics 88 (3), 333-346 (2006)
   PUBMED   16730941
REFERENCE   10 (bases 1 to 1757)
  AUTHORS   Turpaev,K., Bouton,C., Diet,A., Glatigny,A. and Drapier,J.C.
  TITLE     Analysis of differentially expressed genes in nitric oxide-exposed
            human monocytic cells
  JOURNAL   Free Radic Biol Med 38 (10), 1392-1400 (2005)
   PUBMED   15855057
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC013356.8.
            
            On Feb 11, 2021 this sequence version replaced NM_033286.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK301887.1, BC064344.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249776.13/ ENSP00000249776.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-324               AC013356.8         68445-68768
            325-419             AC013356.8         68952-69046
            420-552             AC013356.8         72086-72218
            553-600             AC013356.8         72883-72930
            601-706             AC013356.8         75230-75335
            707-800             AC013356.8         75560-75653
            801-862             AC013356.8         77217-77278
            863-937             AC013356.8         77673-77747
            938-1757            AC013356.8         79193-80012
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..1757
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /note="kinetochore localized astrin (SPAG5) binding
                     protein"
                     /db_xref="GeneID:90417"
                     /db_xref="HGNC:HGNC:30767"
                     /db_xref="MIM:614718"
     exon            1..324
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1066
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /note="isoform a is encoded by transcript variant 1;
                     putative TRAF4-associated factor 1; small kinetochore
                     associated protein; TRAF4 associated factor 1; kinastrin;
                     kinetochore-localized astrin-binding protein; small
                     kinetochore-associated protein, kinetochore-localized
                     astrin-binding protein, TRAF4 associated factor 1"
                     /codon_start=1
                     /product="small kinetochore-associated protein isoform a"
                     /protein_id="NP_150628.3"
                     /db_xref="CCDS:CCDS42021.1"
                     /db_xref="GeneID:90417"
                     /db_xref="HGNC:HGNC:30767"
                     /db_xref="MIM:614718"
                     /translation="MAAPEAPPLDRVFRTTWLSTECDSHPLPPSYRKFLFETQAADLA
                     GGTTVAAGNLLNESEKDCGQDRRAPGVQPCRLVTMTSVVKTVYSLQPPSALSGGQPAD
                     TQTRATSKSLLPVRSKEVDVSKQLHSGGPENDVTKITKLRRENGQMKATDTATRRNVR
                     KGYKPLSKQKSEEELKDKNQLLEAVNKQLHQKLTETQGELKDLTQKVELLEKFRDNCL
                     AILESKGLDPALGSETLASRQESTTDHMDSMLLLETLQEELKLFNETAKKQMEELQAL
                     KVKLEMKEERVRFLEQQTLCNNQVNDLTTALKEMEQLLEM"
     exon            325..419
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..552
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            553..600
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            601..706
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..800
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            801..862
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            863..937
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1757
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1695..1700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /note="hexamer: AATAAA"
     polyA_site      1714
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /note="major polyA site"
     regulatory      1735..1740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
                     /note="hexamer: AATGAA"
     polyA_site      1757
                     /gene="KNSTRN"
                     /gene_synonym="C15orf23; HSD11; ROCHIS; SKAP; TRAF4AF1"
ORIGIN      
        1 attagctcca ttcaagccta caaattgcat caccctcctc ctctgcccag acctgggggc
       61 tccaacacct ttcgctaggt ctggctctgg cctctgagcg aaccttccgt acagtatggc
      121 ggctcccgaa gccccgcccc tggacagagt tttccgtaca acatggctgt ctacagagtg
      181 cgattcccac ccacttccgc ctagctaccg gaagtttcta tttgaaaccc aggcggccga
      241 cttagccggt ggcacgacag ttgctgcagg gaatctttta aacgagagcg agaaggactg
      301 cgggcaggac cggcgggctc ctggggttca gccgtgccgc ctcgttacga tgaccagtgt
      361 ggttaagaca gtgtatagcc tgcagccccc ctctgcgctg agcggcggcc agccggcaga
      421 cacacaaact cgggccactt ctaagagtct cttacctgtt aggtccaaag aagtcgatgt
      481 ttccaaacag cttcattcag gaggtccaga gaatgatgtt acaaaaatca ccaaactgag
      541 acgagagaat gggcaaatga aagctactga cactgccacc agaaggaatg tcagaaaagg
      601 ctacaaacca ctgagtaagc aaaaatcaga ggaagagctc aaggacaaga accagctgtt
      661 agaagccgtc aacaagcagt tgcaccagaa gttgactgaa actcagggag agctgaagga
      721 cctgacccag aaggtagagc tgctggagaa gtttcgggac aactgtttgg caattttgga
      781 gagcaagggc cttgatccag ctttaggcag tgagaccctg gcatcacgac aagaatccac
      841 tactgatcac atggactcta tgttgctgtt agaaactttg caagaggagc tgaagctttt
      901 taacgaaaca gccaaaaagc agatggagga gttacaggcc ttaaaggtaa agctggagat
      961 gaaagaggaa agagtccgat tcctagaaca gcaaacctta tgtaacaatc aagtaaatga
     1021 tttaacaaca gcccttaagg aaatggagca gctattagaa atgtaagaag aagcaagtgg
     1081 ccagatggct ccctcttggg cataaaatct cagaggaagc tacttaggac atcatcttgg
     1141 ccatgatctt ctgggactca ccatctccag aatgaaaaca atttctacag tagacttaag
     1201 gacagtttat gctgaaatgg caattcctca tttaagcaag ttttcccaac cttcaggttg
     1261 gtcagccctc ctgagcctca caggtggata attgaggcct acaagagagg ggagcctagg
     1321 agcttggatt gaccttctag tcaaccacct gacttcagca caccattaca atcgggagac
     1381 taaaccaaca accagaggat ctaaaatgtc acattcagat tttcaggaag aaaatcttca
     1441 ttacagtgga gcacaaatgt tccatacaag acatcattga ggagccatgc tgtccccttc
     1501 taacctgaaa cacattcttt cccatcctgg ttgggcttct gtacctcctt attaatttat
     1561 gaacctgaag ttgcttgaag tgttttgggc ttaataaatg gggtgaaagt ataggtagca
     1621 gtaacaccta catgaaacaa tacaccttgg atcttttaat ctaaattact tttctttttt
     1681 aagtctactt ttaaaataaa tacttctgta aatattctga ctgtaacatt gaggaatgaa
     1741 aatagccttt taaccta
//
