LOCUS       NM_144508               9266 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens kinetochore scaffold 1 (KNL1), transcript variant 2,
            mRNA.
ACCESSION   NM_144508
VERSION     NM_144508.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Fellows,B.J., Tolezano,G.C., Pires,S.F., Ruegg,M.S.G., Knapp,K.M.,
            Krepischi,A.C.V. and Bicknell,L.S.
  TITLE     A novel KNL1 intronic splicing variant likely destabilizes the KMN
            complex, causing primary microcephaly
  JOURNAL   Am J Med Genet A 194 (3), e63468 (2024)
   PUBMED   37937525
  REMARK    GeneRIF: A novel KNL1 intronic splicing variant likely destabilizes
            the KMN complex, causing primary microcephaly.
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Wu,L., Zhang,G., Zhu,Q. and Huang,Y.
  TITLE     Kinetochore scaffold 1 downregulation suppressed the development of
            non-small cell lung cancer by inactivating the phosphatidylinositol
            3 kinase/protein kinase B (AKT)/nuclear factor-kappa B pathway
  JOURNAL   J Physiol Pharmacol 75 (1) (2024)
   PUBMED   38583438
  REMARK    GeneRIF: Kinetochore scaffold 1 downregulation suppressed the
            development of non-small cell lung cancer by inactivating the
            phosphatidylinositol 3 kinase/protein kinase B (AKT)/nuclear
            factor-kappa B pathway.
REFERENCE   3  (bases 1 to 9266)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 9266)
  AUTHORS   Kuefer,M.U., Chinwalla,V., Zeleznik-Le,N.J., Behm,F.G., Naeve,C.W.,
            Rakestraw,K.M., Mukatira,S.T., Raimondi,S.C. and Morris,S.W.
  TITLE     Characterization of the MLL partner gene AF15q14 involved in
            t(11;15)(q23;q14)
  JOURNAL   Oncogene 22 (9), 1418-1424 (2003)
   PUBMED   12618768
  REMARK    GeneRIF: AF15q14 seems identical to an mRNA previously found to be
            expressed in melanoma rendered nontumorigenic by microcell-mediated
            introduction of normal chromosome 6, suggesting the gene may
            function normally to suppress cell growth and/or enhance
            maturation.
REFERENCE   5  (bases 1 to 9266)
  AUTHORS   Chinwalla,V., Chien,A., Odero,M., Neilly,M.B., Zeleznik-Le,N.J. and
            Rowley,J.D.
  TITLE     A t(11;15) fuses MLL to two different genes, AF15q14 and a novel
            gene MPFYVE on chromosome 15
  JOURNAL   Oncogene 22 (9), 1400-1410 (2003)
   PUBMED   12618766
REFERENCE   6  (bases 1 to 9266)
  AUTHORS   Takimoto,M., Wei,G., Dosaka-Akita,H., Mao,P., Kondo,S.,
            Sakuragi,N., Chiba,I., Miura,T., Itoh,N., Sasao,T., Koya,R.C.,
            Tsukamoto,T., Fujimoto,S., Katoh,H. and Kuzumaki,N.
  TITLE     Frequent expression of new cancer/testis gene D40/AF15q14 in lung
            cancers of smokers
  JOURNAL   Br J Cancer 86 (11), 1757-1762 (2002)
   PUBMED   12087463
  REMARK    GeneRIF: a new cancer/testis gene frequently expressed in lung
            cancer of smokers
REFERENCE   7  (bases 1 to 9266)
  AUTHORS   Hayette,S., Tigaud,I., Vanier,A., Martel,S., Corbo,L., Charrin,C.,
            Beillard,E., Deleage,G., Magaud,J.P. and Rimokh,R.
  TITLE     AF15q14, a novel partner gene fused to the MLL gene in an acute
            myeloid leukaemia with a t(11;15)(q23;q14)
  JOURNAL   Oncogene 19 (38), 4446-4450 (2000)
   PUBMED   10980622
REFERENCE   8  (bases 1 to 9266)
  AUTHORS   Wei,G., Takimoto,M., Yoshida,I., Mao,P.Z., Koya,R.C., Miura,T. and
            Kuzumaki,N.
  TITLE     Chromosomal assignment of a novel human gene D40
  JOURNAL   Nucleic Acids Symp Ser (42), 71-72 (1999)
   PUBMED   10780384
REFERENCE   9  (bases 1 to 9266)
  AUTHORS   Ray,M.E., Su,Y.A., Meltzer,P.S. and Trent,J.M.
  TITLE     Isolation and characterization of genes associated with
            chromosome-6 mediated tumor suppression in human malignant melanoma
  JOURNAL   Oncogene 12 (12), 2527-2533 (1996)
   PUBMED   8700511
REFERENCE   10 (bases 1 to 9266)
  AUTHORS   Verloes,A., Drunat,S., Gressens,P. and Passemard,S.
  TITLE     Primary Autosomal Recessive Microcephalies and Seckel Syndrome
            Spectrum Disorders - RETIRED CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301772
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC014306.1, AF461041.1,
            AC022405.5 and AB046790.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_144508.4.
            
            Summary: The protein encoded by this gene is a component of the
            multiprotein assembly that is required for creation of
            kinetochore-microtubule attachments and chromosome segregation. The
            encoded protein functions as a scaffold for proteins that influence
            the spindle assembly checkpoint during the eukaryotic cell cycle
            and it interacts with at least five different kinetochore proteins
            and two checkpoint kinases. In adults, this gene is predominantly
            expressed in normal testes, various cancer cell lines and primary
            tumors from other tissues and is ubiquitously expressed in fetal
            tissues. This gene was originally identified as a fusion partner
            with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14).
            Mutations in this gene cause autosomal recessive primary
            microcephaly-4 (MCPH4). Alternative splicing results in multiple
            transcript variants encoding different isoforms. Additional splice
            variants have been described but their biological validity has not
            been confirmed. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF461041.1, SRR14038193.3052100.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399668.7/ ENSP00000382576.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BC014306.1         4-19
            17-1538             AF461041.1         1-1522
            1539-2889           AC022405.5         31644-32994
            2890-7126           AF461041.1         2874-7110
            7127-7607           AB046790.1         4099-4579
            7608-9266           AC022405.5         72686-74344
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..9266
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /note="kinetochore scaffold 1"
                     /db_xref="GeneID:57082"
                     /db_xref="HGNC:HGNC:24054"
                     /db_xref="MIM:609173"
     exon            1..144
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            145..196
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..7112
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /note="isoform 2 is encoded by transcript variant 2;
                     kinetochore null 1 homolog; blinkin, bub-linking
                     kinetochore protein; cancer/testis antigen 29;
                     kinetochore-null protein 1; cancer susceptibility
                     candidate gene 5 protein; ALL1-fused gene from chromosome
                     15q14 protein; protein phosphatase 1, regulatory subunit
                     55; microcephaly, primary autosomal recessive 4; cancer
                     susceptibility candidate 5; outer kinetochore KNL1 complex
                     subunit KNL1"
                     /codon_start=1
                     /product="outer kinetochore KNL1 complex subunit KNL1
                     isoform 2"
                     /protein_id="NP_653091.3"
                     /db_xref="CCDS:CCDS42024.1"
                     /db_xref="GeneID:57082"
                     /db_xref="HGNC:HGNC:24054"
                     /db_xref="MIM:609173"
                     /translation="MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERV
                     QESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEETETGENLLLIQNKKLEDN
                     YCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVM
                     ITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDF
                     IKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFRE
                     KDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATG
                     NFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMG
                     AETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEK
                     NLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQI
                     AAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTEL
                     LSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQ
                     RSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQ
                     ITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSL
                     SNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMN
                     RQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGP
                     KIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDM
                     EITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDR
                     PNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEV
                     ADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDI
                     TQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEI
                     AIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKD
                     NSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVD
                     QACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAM
                     LLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQ
                     LDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDD
                     IYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILN
                     SEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVP
                     CFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINI
                     ISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGI
                     DDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSS
                     DSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKF
                     SDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRS
                     SQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTP
                     PTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRH
                     CSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMD
                     KILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLL
                     ELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTI
                     TFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTC
                     TTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAA
                     FAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQ
                     DLFQDCHFYH"
     exon            197..236
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            237..296
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            297..358
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            359..411
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            412..445
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            446..483
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            484..536
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            537..5537
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            5538..5676
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            5677..5744
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            5745..5843
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            5844..5959
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            5960..6050
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6051..6167
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6168..6255
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6256..6333
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6334..6373
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6374..6475
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6476..6576
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6577..6645
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6646..6755
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6756..6874
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6875..6997
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     exon            6998..9266
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9247..9252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /note="hexamer: AATAAG"
     polyA_site      9266
                     /gene="KNL1"
                     /gene_synonym="AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105;
                     MCPH4; PPP1R55; Spc7"
                     /note="major polyA site"
ORIGIN      
        1 agatttgaat acttcactga ggcgagccgg gcgttgtgag cggactgcta gaggcggctg
       61 tctgtttccg ctctaaggaa actcagagcg tgtggacccc aaacaagtct gcgcaaaatt
      121 tgtcgaggag gtttgccgcg gcagaaaagt tttcttcaaa aatggatggg gtgtcttcag
      181 aggctaatga agaaaatgac aatatagaga gacctgttag aagacggcat tcttcaatat
      241 tgaaaccccc aaggagtcct cttcaggacc tcagaggtgg gaatgaaaga gttcaggaat
      301 ccaatgcttt gagaaataag aaaaactctc gtcgagtcag ctttgcagat actataaagg
      361 tattccagac ggagtctcat atgaaaatag tgagaaagtc agaaatggaa gaaacagaaa
      421 caggagaaaa tcttcttttg atacagaata agaaattaga agataattac tgtgaaatta
      481 ctgggatgaa cacattgctt tctgctccca ttcataccca gatgcaacag aaggagtttt
      541 caattataga acatacccgt gaaaggaaac atgcaaatga ccagacagtc attttttcag
      601 atgaaaacca gatggacctg acatcaagtc acactgtaat gattaccaaa ggccttttag
      661 ataatcccat aagtgaaaag tccaccaaga tagataccac atcatttcta gctaatttaa
      721 agcttcacac cgaggactca agaatgaaaa aagaagtaaa tttttccgtg gatcaaaaca
      781 cttcttcaga aaataaaata gatttcaatg acttcataaa aagattgaaa acaggaaaat
      841 gtagtgcttt tcctgatgtg cctgataaag aaaattttga gatacctatt tattccaagg
      901 aaccgaacag tgcctcttct acacatcaaa tgcatgtatc tcttaaggaa gatgaaaata
      961 acagtaatat tactaggctc tttagagaaa aagatgatgg gatgaatttc acccagtgtc
     1021 atacagccaa tattcagaca ttgattccca catccagtga gaccaactca cgggaatcta
     1081 aaggtaatga tattacaatt tatggcaatg actttatgga cttgacattt aaccacactt
     1141 tgcagatctt acctgcaaca ggtaattttt ctgaaataga aaatcaaact cagaatgcca
     1201 tggatgtaac aacaggttat ggaactaaag cttcaggaaa taaaacagtt tttaagagta
     1261 aacaaaatac tgcttttcaa gacctttcca taaactctgc agacaaaata catattacca
     1321 gaagtcatat tatgggggca gaaactcaca tagtctcaca gacttgtaat caggatgcca
     1381 gaatattagc catgacccca gaatctatat attctaatcc atctattcaa ggttgtaaga
     1441 ctgttttcta ttctagttgt aatgatgcca tggaaatgac caaatgtctc tcaaatatga
     1501 gagaggagaa aaatttgcta aagcatgaca gtaattatgc taaaatgtat tgcaatccag
     1561 atgctatgtc ttctctcaca gagaaaacta tttattccgg agaggagaac atggacatta
     1621 ccaagagtca tacagttgca atagataatc aaatttttaa acaagatcaa tcaaatgtgc
     1681 aaatagcagc tgcaccaaca cccgaaaaag aaatgatgct ccaaaatctt atgaccacat
     1741 cagaagatgg gaaaatgaat gtaaattgta actcagttcc tcatgtatct aaggaaagaa
     1801 tacagcagag cctgtcaaat cctttgtcta tttcattgac tgatagaaag actgaactct
     1861 tatcaggtga aaatatggat ttgactgaaa gtcacacaag taacttagga agtcaggttc
     1921 ctcttgcagc ttataatcta gcaccggaga gtaccagtga atctcactct cagagcaaaa
     1981 gctcttcaga tgaatgtgaa gaaattacca aaagtcgtaa tgaaccattt cagcgatcag
     2041 acataatagc caaaaacagc ttaaccgaca cctggaacaa agacaaagat tgggttttga
     2101 agattttgcc ctaccttgat aaagattctc ctcagtcagc tgattgtaat caggagatag
     2161 caacaagcca taatatagtc tactgtggtg gagttcttga taaacaaata actaatagaa
     2221 atacagtatc atgggaacaa tctttgtttt ctaccacaaa gccattattt tcatcaggac
     2281 agttctctat gaaaaatcat gatactgcta taagtagtca tacagtgaaa tctgtactag
     2341 gccagaattc taaactggct gagccactga ggaaaagttt aagcaatccc acacctgact
     2401 attgccatga caagatgatt atatgttcag aggaagagca aaatatggat ctaacaaaga
     2461 gccacactgt cgtcattgga tttggtcctt ctgaactaca agaacttggt aaaactaatt
     2521 tagaacacac tactggccag ctaacaacaa tgaacagaca gatagctgta aaagttgaaa
     2581 aatgtggtaa aagtcccata gaaaaaagtg gagtgcttaa atctaactgt attatggatg
     2641 tgttagagga cgaaagtgta cagaaaccta aatttccaaa ggaaaagcaa aatgtcaaaa
     2701 tttggggaag gaaaagtgtt ggtggaccaa aaattgataa gactattgta ttttcagaag
     2761 acgataagaa tgatatggat atcactaaga gttatacaat agaaataaac catagacctt
     2821 tattagagaa acgtgattgt catttggtgc cattggcagg aacttctgaa actattttat
     2881 atacatgtag gcaggatgac atggagatca ctagaagtca cacaactgcc ttagaatgta
     2941 aaactgtctc accagatgaa ataactacta ggcctatgga caaaactgta gtgtttgtag
     3001 ataatcatgt tgaactagaa atgacagagt cccatactgt tttcattgac taccaagaaa
     3061 aggaaagaac agacagacct aactttgaac tatcccaaag gaaaagccta ggaacaccaa
     3121 cagtgatatg tactcctact gaggagagtg ttttctttcc aggaaatggt gaaagtgacc
     3181 gtctagtagc aaatgacagc cagctaaccc ctctggagga atggtctaat aataggggcc
     3241 ctgtagaggt agctgataac atggaattgt ctaaatcagc cacttgcaaa aacatcaaag
     3301 atgtacaaag tcctggattt ctgaatgaac ctctatcaag caaaagtcag agaagaaaaa
     3361 gccttaagct aaaaaatgac aagaccattg tattttcaga gaatcataaa aatgatatgg
     3421 atattaccca gagttgtatg gtggaaatag ataacgaaag tgccctggag gataaagagg
     3481 acttccattt ggcaggggct tctaaaacta ttttgtattc atgtgggcag gatgacatgg
     3541 agatcactag gagtcacaca actgccttag aatgtaaaac tctcctgcca aatgaaatag
     3601 ctattaggcc catggacaaa accgtattgt tcacagataa ttacagtgat ctggaagtca
     3661 ccgattccca tactgttttc attgactgtc aagccacaga gaaaatactt gaagaaaacc
     3721 ctaaatttgg aataggaaaa ggaaaaaact tgggtgtttc ctttcctaag gataatagct
     3781 gtgttcaaga aatcgctgaa aaacaagcac tggctgtagg aaacaaaata gttcttcaca
     3841 ccgagcaaaa gcaacaactc tttgctgcta ctaatagaac tactaatgaa atcatcaaat
     3901 ttcatagtgc tgctatggat gaaaaggtca tagggaaagt tgtagaccag gcctgtacat
     3961 tggaaaaagc gcaagttgaa agctgtcagt taaataatag agatagaaga aatgtggact
     4021 ttacaagtag tcatgcaact gctgtttgtg gatccagtga taattattcc tgtttaccaa
     4081 atgttatttc ctgtactgat aatttggagg gtagtgccat gctcttatgt gataaagatg
     4141 aggaaaaagc caattattgc ccagtgcaaa atgatcttgc ttatgcaaat gattttgcca
     4201 gtgaatatta cttggaatct gagggacagc ctctctctgc tccttgtcct ttgttagaga
     4261 aggaagaagt tattcaaacc agtaccaaag gacagttaga ctgtgttata acactgcaca
     4321 aagatcaaga tctgattaag gatccacgaa atctattggc taatcaaact ttagtatata
     4381 gtcaagatct gggggagatg actaaactta attcaaagcg agtatctttt aagcttccaa
     4441 aggatcaaat gaaagtctat gttgatgaca tttatgttat tcctcagcct catttctcaa
     4501 ccgaccaacc tccattacct aaaaaaggac agagtagtat caataaagaa gaagtaatac
     4561 tgtctaaagc tggaaataag agtttaaata ttatagaaaa ttcctctgca cccatatgtg
     4621 aaaacaagcc caaaatactc aatagtgagg aatggtttgc tgcagcctgt aaaaaagaac
     4681 tgaaggaaaa tattcaaaca actaactata atacagctct agatttccac agtaactcag
     4741 acgtaactaa gcaagtcatt caaactcatg tcaatgctgg agaagcacca gatcctgtaa
     4801 ttacatctaa tgttccatgt tttcatagta tcaaaccaaa tctgaataat ttgaatggaa
     4861 aaactggaga gtttttagcc tttcaaactg ttcatctacc accccttcca gagcaattac
     4921 ttgaattagg aaataaggca cacaatgata tgcatatagt gcaagctaca gaaatacata
     4981 atattaacat aatctccagc aatgctaaag atagtagaga tgaggaaaat aaaaagtctc
     5041 ataatggagc tgaaaccacc tctctaccgc caaagacagt ttttaaagat aaagtaagga
     5101 gatgttcttt gggaatcttt ttgcctagat tgcccaacaa gagaaattgt agtgtcactg
     5161 gtattgatga cctggaacag attccagcag acacaactga tataaatcac ttagaaactc
     5221 agccggtctc tagcaaagat tcaggcattg gatctgttgc aggtaaactg aacctaagtc
     5281 cttctcaata tataaatgag gaaaatcttc ctgtatatcc tgatgagatc aattcttcag
     5341 actctattaa catagaaact gaggaaaagg ccttgattga gacataccaa aaagagattt
     5401 caccatatga aaataaaatg ggaaaaactt gcaatagcca aaaaagaacg tgggtacaag
     5461 aagaagaaga tattcataag gagaaaaaaa tcagaaaaaa tgagattaag tttagtgata
     5521 cgacacaaga tcgggagatt tttgatcacc atactgaaga ggatatagat aaaagtgcta
     5581 acagtgtatt gataaaaaac ctgagcagga ccccatctag ttgcagcagc tctctggatt
     5641 caatcaaggc tgatgggacc tctctggact tcagcactta ccgcagtagt caaatggaat
     5701 cacagtttct cagagatact atttgtgaag agagcttgag ggagaaactc caagatggga
     5761 gaataacaat aagggagttc tttatacttc tccaggtcca catcttgata cagaaacccc
     5821 gacagagcaa tctcccaggc aattttactg taaacacacc acctactcca gaagacctga
     5881 tgttaagtca atatgtttac cgacccaaga tacagattta tagagaagat tgtgaggctc
     5941 gtcgccaaaa gattgaagaa ttaaagcttt ctgcatcgaa ccaagataag ctgttggttg
     6001 atataaataa gaacctgtgg gaaaaaatga gacactgctc tgacaaagag ctgaaggcct
     6061 ttggaattta tcttaacaaa ataaagtcat gttttaccaa gatgactaaa gtcttcactc
     6121 accaaggaaa agtggctctg tatggcaagc tggtgcagtc agctcagaat gagagggaga
     6181 aacttcaaat aaagatagat gagatggata aaatacttaa gaagatcgat aactgcctca
     6241 ctgagatgga aacagaaact aagaatttgg aggatgaaga gaaaaacaat cctgtggaag
     6301 aatgggattc tgaaatgaga gctgcagaaa aagaattgga acagctgaaa actgaagaag
     6361 aggagcttca aagaaatctc ttagaactgg aggtacaaaa agagcagacc cttgctcaaa
     6421 tagactttat gcaaaaacaa agaaatagaa ctgaagagct actggatcag ttgagcttgt
     6481 ctgagtggga tgtcgttgag tggagtgatg atcaagctgt attcaccttt gtttatgaca
     6541 cgatacaact caccatcacc tttgaagagt cagttgttgg tttccctttc ctggacaagc
     6601 gttataggaa gattgttgat gtcaattttc aatctctgtt agatgaggat caagctcctc
     6661 cttcctccct tttagttcat aagcttattt tccagtacgt tgaagaaaag gaatcctgga
     6721 agaagacatg tacaacccag catcagttac ccaagatgct tgaagaattc tcactggtag
     6781 tgcaccattg cagactcctt ggagaggaga ttgagtattt aaagagatgg ggaccaaatt
     6841 ataacctaat gaacatagat attaataata atgaattgag acttttattc tctagctccg
     6901 cagcatttgc aaagtttgaa ataactttgt ttctctcagc ctattatcca tctgtaccat
     6961 taccttccac cattcagaat cacgttggga acactagcca agatgatatt gctaccattc
     7021 tatctaaagt gccactggag aacaactacc tgaagaatgt agtcaagcaa atttaccaag
     7081 atctgtttca ggactgccat ttctaccact agacccttgg accaccattg gaacaaccaa
     7141 gcagaatgta cttgatatta tttcagggtc ccattgctgt tcagcctttg tttttacgtc
     7201 attacaagct gagtaaaatt ccttctgatg atgttatagt taatctgtat gttttttata
     7261 tctctgcaga atgatggtga tgaagtctgg atggtaggcc tcatagccta ctatcaactt
     7321 actcatcttt gtaccaaagg tttaagtaat aggacactta ggaaaaatgt ctcctaacta
     7381 aactagtgct ttctgcttta gtacaagccc taaggattaa cttaagtata agaagtgtta
     7441 tcactgacaa gaacattagc cattttccca taactagata gagctatgat tttttaggtt
     7501 gcctggcttc tgcctagcag atatttctgg agtagaaatg tatctgtcta caaactatta
     7561 tcctttttct ccgttactaa aatgctatta agagaaagta gggctgggtg cggtggctca
     7621 cacctgtaat accagcagtt tgggaggcca aaatgggtga atagcctgag tccaggaatt
     7681 caagaccagc ctgggcaaca tggcaaaacc ccacctctac aaaaaatacg aaagttagcc
     7741 agtcatggtg gcttgcgcct gtggtcccag ctacttagga gcttaaggca ggaggatcac
     7801 ttgagcccag gaggcagagg ttgcagtaag ccgagaccat tccactgcac tccagcctag
     7861 gcaacagagc aagaccctgt ctcaaaaaaa caaaaagaga gaaagcaata gcactcagct
     7921 attgaaatgg ttgccatctt tttttgaagg ccttgctttt tattctatgg tgtctattat
     7981 gttttctttt tctttttttt tgagacggag tcttgctctg tcgcccaggc tggagtgcag
     8041 tggcatgatc tcagctcact gcaagctccg cctcctgggt tcacgccatt ctcctgcctc
     8101 agcctcccga gtagctggga ctacaggtgc ccaccaccat gccgggctaa ttttttgtct
     8161 ttttagtaga aatggggttt cacgatgtta gccaggatgg tctcgatctc ctgacctcgt
     8221 gatccgcctg cctcggcttc aaagtgctgg gattacaggt gtgagccacc acacccagca
     8281 atgttttctt aataagtata gtttttctag ggaaagttaa ttcatttttg tctagtacat
     8341 atatgtaaat atattaatgt tgtttttgtg tttgtgatgt agtaaggaga tgtacataga
     8401 aattcattga ggtatataga tactcatctg tctaggcagt tcccaatttt ctgaagaatg
     8461 ttttacagca aaattttcta ttttctttta ttaaatagtg acacgtcaaa caatgtcaca
     8521 tccaaaacac tagtttcatc aatttctagc agtaataata gacttgctgt aagtattgtt
     8581 ttctgatgcc atacccttgt catacatatt attaaatgac caatattatg tatgaagtag
     8641 acaaaaaaat ttactcaaac ttcattcaaa tcctaattgt gataattttt gttttatatt
     8701 taattataaa ccaaaataca tttgcatttt taagctaatt tgtctcaaaa ttttgcttta
     8761 tatttttgga tcaggttaaa gtcctgtgga tcccctgaat gttattgtcc ctcttgattg
     8821 gtttttactt ctgagctata cgtcaaaaga cacataagct tcaaaagtca agacaaacct
     8881 catttgccat aaaaatcaag atatagatgt tctgttccgt aaactccttg aaaaacattt
     8941 taaagtcatc aatatgatct gtttcccatg aaacttaagt tagctttctt attggagtta
     9001 tttcttttct gtaagtctga aaagtagaga ttttgtttta cgcattttag taacctgcaa
     9061 caaccaactc taaaaaagat ttggcttgta atgacggtct ctgctttttt gggtttggag
     9121 tacacaattg taatatttac ttagttattt gtgtttttct ttgttcaagg tattgactag
     9181 tttcataaat tttttgaaag tttttctttc attggttgga aagcagatta cattttgcac
     9241 tattaaaata agtttattac tttaaa
//
