LOCUS       NM_012315               1401 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens kallikrein related peptidase 9 (KLK9), mRNA.
ACCESSION   NM_012315
VERSION     NM_012315.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Adamopoulos,P.G., Kontos,C.K. and Scorilas,A.
  TITLE     Molecular cloning of novel transcripts of human kallikrein-related
            peptidases 5, 6, 7, 8 and 9 (KLK5 - KLK9), using Next-generation
            sequencing
  JOURNAL   Sci Rep 7 (1), 17299 (2017)
   PUBMED   29229980
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Geng,X., Liu,Y., Diersch,S., Kotzsch,M., Grill,S., Weichert,W.,
            Kiechle,M., Magdolen,V. and Dorn,J.
  TITLE     Clinical relevance of kallikrein-related peptidase 9, 10, 11, and
            15 mRNA expression in advanced high-grade serous ovarian cancer
  JOURNAL   PLoS One 12 (11), e0186847 (2017)
   PUBMED   29095848
  REMARK    GeneRIF: Pronounced correlations between KLK10/KLK11 (rs = 0.647)
            and between KLK9/KLK15 (rs = 0.716) mRNA, but not between other
            combinations, indicate coordinate expression of distinct pairs of
            peptidases
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1401)
  AUTHORS   Filippou,P.S., Farkona,S., Brinc,D., Yu,Y., Prassas,I. and
            Diamandis,E.P.
  TITLE     Biochemical and functional characterization of the human tissue
            kallikrein 9
  JOURNAL   Biochem J 474 (14), 2417-2433 (2017)
   PUBMED   28559305
  REMARK    GeneRIF: Data suggest that mature KLK9 (kallikrein 9) is a
            glycosylated chymotrypsin-like enzyme with strong preference for
            tyrosine over phenylalanine at P1 cleavage position; substrate
            specificity of KLK9 appears to extend to KLK10 and midkine; enzyme
            activity is enhanced by Mg2+ and Ca2+, but is reversibly attenuated
            by Zn2+; KLK9 is inhibited in vitro by many naturally occurring or
            synthetic protease inhibitors.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1401)
  AUTHORS   Drucker,K.L., Gianinni,C., Decker,P.A., Diamandis,E.P. and
            Scarisbrick,I.A.
  TITLE     Prognostic significance of multiple kallikreins in high-grade
            astrocytoma
  JOURNAL   BMC Cancer 15, 565 (2015)
   PUBMED   26231762
  REMARK    GeneRIF: Data demonstrate that elevated levels of KLK6, KLK7 and
            KLK9 proteins are associated with poor glioblastoma patients
            survival.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1401)
  AUTHORS   Nasim,F.U., Ejaz,S., Ashraf,M. and Ahmad,S.
  TITLE     Human tissue kallikrein 9 in asthmatic patients
  JOURNAL   Ann Allergy Asthma Immunol 114 (4), 347-349 (2015)
   PUBMED   25747782
  REMARK    GeneRIF: the serum level has the potential to be used as a
            biomarker for asthma
REFERENCE   6  (bases 1 to 1401)
  AUTHORS   Lundwall,A., Band,V., Blaber,M., Clements,J.A., Courty,Y.,
            Diamandis,E.P., Fritz,H., Lilja,H., Malm,J., Maltais,L.J.,
            Olsson,A.Y., Petraki,C., Scorilas,A., Sotiropoulou,G.,
            Stenman,U.H., Stephan,C., Talieri,M. and Yousef,G.M.
  TITLE     A comprehensive nomenclature for serine proteases with homology to
            tissue kallikreins
  JOURNAL   Biol Chem 387 (6), 637-641 (2006)
   PUBMED   16800724
REFERENCE   7  (bases 1 to 1401)
  AUTHORS   Yousef,G.M., Kyriakopoulou,L.G., Scorilas,A., Fracchioli,S.,
            Ghiringhello,B., Zarghooni,M., Chang,A., Diamandis,M., Giardina,G.,
            Hartwick,W.J., Richiardi,G., Massobrio,M., Diamandis,E.P. and
            Katsaros,D.
  TITLE     Quantitative expression of the human kallikrein gene 9 (KLK9) in
            ovarian cancer: a new independent and favorable prognostic marker
  JOURNAL   Cancer Res 61 (21), 7811-7818 (2001)
   PUBMED   11691797
REFERENCE   8  (bases 1 to 1401)
  AUTHORS   Gan,L., Lee,I., Smith,R., Argonza-Barrett,R., Lei,H., McCuaig,J.,
            Moss,P., Paeper,B. and Wang,K.
  TITLE     Sequencing and expression analysis of the serine protease gene
            cluster located in chromosome 19q13 region
  JOURNAL   Gene 257 (1), 119-130 (2000)
   PUBMED   11054574
REFERENCE   9  (bases 1 to 1401)
  AUTHORS   Yousef,G.M. and Diamandis,E.P.
  TITLE     The expanded human kallikrein gene family: locus characterization
            and molecular cloning of a new member, KLK-L3 (KLK9)
  JOURNAL   Genomics 65 (2), 184-194 (2000)
   PUBMED   10783266
REFERENCE   10 (bases 1 to 1401)
  AUTHORS   Yousef,G.M., Luo,L.Y. and Diamandis,E.P.
  TITLE     Identification of novel human kallikrein-like genes on chromosome
            19q13.3-q13.4
  JOURNAL   Anticancer Res 19 (4B), 2843-2852 (1999)
   PUBMED   10652563
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011473.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 26, 2018 this sequence version replaced NM_012315.1.
            
            Summary: The protein encoded by this gene is a kallikrein-related
            serine protease. This gene is activated by steroid hormones in a
            human breast cancer cell line, making it a good marker for cancer
            detection. The encoded protein is found primarily in the
            cytoplasm.[provided by RefSeq, Oct 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: DQ267419.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA2145893, SAMEA2147596 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000594211.2/ ENSP00000469417.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AC011473.4         9586-9673           c
            89-245              AC011473.4         9264-9420           c
            246-511             AC011473.4         6539-6804           c
            512-648             AC011473.4         3785-3921           c
            649-1401            AC011473.4         2589-3341           c
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.41"
     gene            1..1401
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /note="kallikrein related peptidase 9"
                     /db_xref="GeneID:284366"
                     /db_xref="HGNC:HGNC:6370"
                     /db_xref="MIM:605504"
     exon            1..88
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..798
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /note="kallikrein-like protein 3"
                     /codon_start=1
                     /product="kallikrein-9 precursor"
                     /protein_id="NP_036447.1"
                     /db_xref="CCDS:CCDS12816.1"
                     /db_xref="GeneID:284366"
                     /db_xref="HGNC:HGNC:6370"
                     /db_xref="MIM:605504"
                     /translation="MKLGLLCALLSLLAGHGWADTRAIGAEECRPNSQPWQAGLFHLT
                     RLFCGATLISDRWLLTAAHCRKPYLWVRLGEHHLWKWEGPEQLFRVTDFFPHPGFNKD
                     LSANDHNDDIMLIRLPRQARLSPAVQPLNLSQTCVSPGMQCLISGWGAVSSPKALFPV
                     TLQCANISILENKLCHWAYPGHISDSMLCAGLWEGGRGSCQGDSGGPLVCNGTLAGVV
                     SGGAEPCSRPRRPAVYTSVCHYLDWIQEIMEN"
     exon            89..245
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /inference="alignment:Splign:2.1.0"
     exon            246..511
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /inference="alignment:Splign:2.1.0"
     exon            512..648
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /inference="alignment:Splign:2.1.0"
     exon            649..1401
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1377..1382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /note="hexamer: AGTAAA"
     polyA_site      1401
                     /gene="KLK9"
                     /gene_synonym="KLK-L3; KLKL3"
                     /note="major polyA site"
ORIGIN      
        1 actggacgcc gggtgcacgt tccaggatcc aggtgcccag gggtcatgaa gctgggactc
       61 ctctgtgctc tgctctctct gctggcaggg catggctggg cagacacccg tgccatcggg
      121 gccgaggaat gtcgccccaa ctcccagcct tggcaggccg gcctcttcca ccttactcgg
      181 ctcttctgtg gggcgaccct catcagtgac cgctggctgc tcacagctgc ccactgccgc
      241 aagccgtatc tgtgggtccg ccttggagag caccacctct ggaaatggga gggtccggag
      301 cagctgttcc gggttacgga cttcttcccc caccctggct tcaacaagga cctcagcgcc
      361 aatgaccaca atgatgacat catgctgatc cgcctgccca ggcaggcacg tctgagtcct
      421 gctgtgcagc ccctcaacct cagccagacc tgtgtctccc caggcatgca gtgtctcatc
      481 tcaggctggg gggccgtgtc cagccccaag gcgctgtttc cagtcacact gcagtgtgcc
      541 aacatcagca tcctggagaa caaactctgt cactgggcat accctggaca catctcggac
      601 agcatgctct gtgcgggcct gtgggagggg ggccgaggtt cctgccaggg tgactctggg
      661 ggccccctgg tttgcaatgg aaccttggca ggcgtggtgt ctgggggtgc tgagccctgc
      721 tccagacccc ggcgccccgc agtctacacc agcgtatgcc actaccttga ctggatccaa
      781 gaaatcatgg agaactgagc ccgcgcgcca cgggggcacc ttggaagacc aagagaggcc
      841 gaagggcacg gggtaggggg ttctcgtagg gtcccagcct caatggttcc cgccctggac
      901 ctccagctgc cctgactccc ctctggacac taagactccg cccctgaggc tccgccccct
      961 cacgaggtca agcaagacac agtcgcgccc cctcggaacg gagcagggac acgcccttca
     1021 gagcccgtct ctatgacgtc accgacagcc atcacctcct tcttggaaca gcacagcctg
     1081 tggctccgcc ccaaggaacc acttacacaa aatagctccg cccctcggaa ctttgcccag
     1141 tgggacttcc cctcgggact ccaccccttg tggccccgcc tccttcacca gagatctcgc
     1201 ccctcgtgat gtcaggggcg cagtagctcc gcccacgtgg agctcgggcg gtgtagagct
     1261 cagccccttg tggccccgtc ctgggcgtgt gctgggtttg aatcctggcg gagacctggg
     1321 gggaaattga gggagggtct ggataccttt agagccaatg caacggatga tttttcagta
     1381 aacgcgggaa acctcacctt c
//
