LOCUS       NM_001648               1464 bp    mRNA    linear   PRI 30-APR-2024
DEFINITION  Homo sapiens kallikrein related peptidase 3 (KLK3), transcript
            variant 1, mRNA.
ACCESSION   NM_001648
VERSION     NM_001648.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Isola,M., Maxia,C., Murtas,D., Ekstrom,J., Isola,R. and Loy,F.
  TITLE     Prostate-specific antigen: An unfamiliar protein in the human
            salivary glands
  JOURNAL   J Anat 244 (5), 873-881 (2024)
   PUBMED   38111134
  REMARK    GeneRIF: Prostate-specific antigen: An unfamiliar protein in the
            human salivary glands.
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Tsuji,K., Kawata,H., Kamiakito,T., Nakaya,T. and Tanaka,A.
  TITLE     RNA-binding protein 14 promotes phase separation to sustain
            prostate specific antigen expression under androgen deprivation in
            human prostate cancer
  JOURNAL   J Steroid Biochem Mol Biol 235, 106407 (2023)
   PUBMED   37806532
  REMARK    GeneRIF: RNA-binding protein 14 promotes phase separation to
            sustain prostate specific antigen expression under androgen
            deprivation in human prostate cancer.
REFERENCE   3  (bases 1 to 1464)
  AUTHORS   Spyratou,V., Freyhult,E., Bergh,A., Thellenberg-Karlsson,C.,
            Wikstrom,P., Welen,K. and Josefsson,A.
  TITLE     Ki67 and prostate specific antigen are prognostic in metastatic
            hormone naive prostate cancer
  JOURNAL   Acta Oncol 62 (12), 1698-1706 (2023)
   PUBMED   37713321
  REMARK    GeneRIF: Ki67 and prostate specific antigen are prognostic in
            metastatic hormone naive prostate cancer.
REFERENCE   4  (bases 1 to 1464)
  AUTHORS   Lehrer,S. and Rheinstein,P.H.
  TITLE     Association of Kallikrein Related Peptidase 3 (KLK3) gene with
            dermatophytosis in the UK biobank cohort
  JOURNAL   Mycoses 66 (12), 1050-1055 (2023)
   PUBMED   37592324
  REMARK    GeneRIF: Association of Kallikrein Related Peptidase 3 (KLK3) gene
            with dermatophytosis in the UK biobank cohort.
REFERENCE   5  (bases 1 to 1464)
  AUTHORS   Mokhtari,H., Ebrahimi,A., Nejati,M., Barartabar,Z., Damchi,M.,
            Khonakdar-Tarsi,A. and Zahedi,M.
  TITLE     The effect of encomir-93 mimic transfection on the expression of
            miR-93 and PSA and androgen receptor in prostate cancer LNcap cell
            line
  JOURNAL   Horm Mol Biol Clin Investig 44 (3), 237-241 (2023)
   PUBMED   36995990
  REMARK    GeneRIF: The effect of encomir-93 mimic transfection on the
            expression of miR-93 and PSA and androgen receptor in prostate
            cancer LNcap cell line.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1464)
  AUTHORS   Cohen,P., Graves,H.C., Peehl,D.M., Kamarei,M., Giudice,L.C. and
            Rosenfeld,R.G.
  TITLE     Prostate-specific antigen (PSA) is an insulin-like growth factor
            binding protein-3 protease found in seminal plasma
  JOURNAL   J Clin Endocrinol Metab 75 (4), 1046-1053 (1992)
   PUBMED   1383255
REFERENCE   7  (bases 1 to 1464)
  AUTHORS   Espana,F., Gilabert,J., Estelles,A., Romeu,A., Aznar,J. and Cabo,A.
  TITLE     Functionally active protein C inhibitor/plasminogen activator
            inhibitor-3 (PCI/PAI-3) is secreted in seminal vesicles, occurs at
            high concentrations in human seminal plasma and complexes with
            prostate-specific antigen
  JOURNAL   Thromb Res 64 (3), 309-320 (1991)
   PUBMED   1725227
REFERENCE   8  (bases 1 to 1464)
  AUTHORS   Christensson,A., Laurell,C.B. and Lilja,H.
  TITLE     Enzymatic activity of prostate-specific antigen and its reactions
            with extracellular serine proteinase inhibitors
  JOURNAL   Eur J Biochem 194 (3), 755-763 (1990)
   PUBMED   1702714
REFERENCE   9  (bases 1 to 1464)
  AUTHORS   Lundwall,A. and Lilja,H.
  TITLE     Molecular cloning of human prostate specific antigen cDNA
  JOURNAL   FEBS Lett 214 (2), 317-322 (1987)
   PUBMED   2436946
REFERENCE   10 (bases 1 to 1413)
  AUTHORS   Lundwall,A. and Lilja,H.
  TITLE     Molecular cloning of human prostate specific antigen cDNA
  JOURNAL   FEBS Lett. 214 (2), 317-322 (1987)
   PUBMED   2436946
REFERENCE   11 (bases 1 to 1464)
  AUTHORS   Watt,K.W., Lee,P.J., M'Timkulu,T., Chan,W.P. and Loor,R.
  TITLE     Human prostate-specific antigen: structural and functional
            similarity with serine proteases
  JOURNAL   Proc Natl Acad Sci U S A 83 (10), 3166-3170 (1986)
   PUBMED   2422647
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X05332.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 12, 2002 this sequence version replaced NM_001648.1.
            
            Summary: Kallikreins are a subgroup of serine proteases having
            diverse physiological functions. Growing evidence suggests that
            many kallikreins are implicated in carcinogenesis and some have
            potential as novel cancer and other disease biomarkers. The gene is
            one of the fifteen kallikrein subfamily members located in a
            cluster on chromosome 19. It encodes a single-chain glycoprotein, a
            protease which is synthesized in the epithelial cells of the
            prostate gland, and is present in seminal plasma. It is thought to
            function normally in the liquefaction of seminal coagulum,
            presumably by hydrolysis of the high molecular mass seminal vesicle
            protein. The serum level of this protein, called PSA in the
            clinical setting, is useful in the diagnosis and monitoring of
            prostatic carcinoma. Alternate splicing of this gene generates
            several transcript variants encoding different isoforms. [provided
            by RefSeq, Dec 2019].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X05332.1, BC056665.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326003.7/ ENSP00000314151.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1464
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /note="kallikrein related peptidase 3"
                     /db_xref="GeneID:354"
                     /db_xref="HGNC:HGNC:6364"
                     /db_xref="MIM:176820"
     exon            1..87
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..827
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /EC_number="3.4.21.77"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; gamma-seminoprotein; semenogelase; seminin;
                     P-30 antigen; prostate-specific antigen; kallikrein-3"
                     /codon_start=1
                     /product="prostate-specific antigen isoform 1
                     preproprotein"
                     /protein_id="NP_001639.1"
                     /db_xref="CCDS:CCDS12807.1"
                     /db_xref="GeneID:354"
                     /db_xref="HGNC:HGNC:6364"
                     /db_xref="MIM:176820"
                     /translation="MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVAS
                     RGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYD
                     MSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSI
                     EPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLV
                     CNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP"
     exon            88..247
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            248..534
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            535..671
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            672..1464
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1443..1448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /note="hexamer: AATAAA"
     polyA_site      1464
                     /gene="KLK3"
                     /gene_synonym="APS; hK3; KLK2A1; PSA"
                     /note="major polyA site"
ORIGIN      
        1 agccccaagc ttaccacctg cacccggaga gctgtgtcac catgtgggtc ccggttgtct
       61 tcctcaccct gtccgtgacg tggattggtg ctgcacccct catcctgtct cggattgtgg
      121 gaggctggga gtgcgagaag cattcccaac cctggcaggt gcttgtggcc tctcgtggca
      181 gggcagtctg cggcggtgtt ctggtgcacc cccagtgggt cctcacagct gcccactgca
      241 tcaggaacaa aagcgtgatc ttgctgggtc ggcacagcct gtttcatcct gaagacacag
      301 gccaggtatt tcaggtcagc cacagcttcc cacacccgct ctacgatatg agcctcctga
      361 agaatcgatt cctcaggcca ggtgatgact ccagccacga cctcatgctg ctccgcctgt
      421 cagagcctgc cgagctcacg gatgctgtga aggtcatgga cctgcccacc caggagccag
      481 cactggggac cacctgctac gcctcaggct ggggcagcat tgaaccagag gagttcttga
      541 ccccaaagaa acttcagtgt gtggacctcc atgttatttc caatgacgtg tgtgcgcaag
      601 ttcaccctca gaaggtgacc aagttcatgc tgtgtgctgg acgctggaca gggggcaaaa
      661 gcacctgctc gggtgattct gggggcccac ttgtctgtaa tggtgtgctt caaggtatca
      721 cgtcatgggg cagtgaacca tgtgccctgc ccgaaaggcc ttccctgtac accaaggtgg
      781 tgcattaccg gaagtggatc aaggacacca tcgtggccaa cccctgagca cccctatcaa
      841 ccccctattg tagtaaactt ggaaccttgg aaatgaccag gccaagactc aagcctcccc
      901 agttctactg acctttgtcc ttaggtgtga ggtccagggt tgctaggaaa agaaatcagc
      961 agacacaggt gtagaccaga gtgtttctta aatggtgtaa ttttgtcctc tctgtgtcct
     1021 ggggaatact ggccatgcct ggagacatat cactcaattt ctctgaggac acagatagga
     1081 tggggtgtct gtgttatttg tggggtacag agatgaaaga ggggtgggat ccacactgag
     1141 agagtggaga gtgacatgtg ctggacactg tccatgaagc actgagcaga agctggaggc
     1201 acaacgcacc agacactcac agcaaggatg gagctgaaaa cataacccac tctgtcctgg
     1261 aggcactggg aagcctagag aaggctgtga gccaaggagg gagggtcttc ctttggcatg
     1321 ggatggggat gaagtaagga gagggactgg accccctgga agctgattca ctatgggggg
     1381 aggtgtattg aagtcctcca gacaaccctc agatttgatg atttcctagt agaactcaca
     1441 gaaataaaga gctgttatac tgtg
//
