LOCUS       NM_020803               5631 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens kelch like family member 8 (KLHL8), transcript variant
            2, mRNA.
ACCESSION   NM_020803
VERSION     NM_020803.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Swaney,D.L., Ramms,D.J., Wang,Z., Park,J., Goto,Y., Soucheray,M.,
            Bhola,N., Kim,K., Zheng,F., Zeng,Y., McGregor,M., Herrington,K.A.,
            O'Keefe,R., Jin,N., VanLandingham,N.K., Foussard,H., Von Dollen,J.,
            Bouhaddou,M., Jimenez-Morales,D., Obernier,K., Kreisberg,J.F.,
            Kim,M., Johnson,D.E., Jura,N., Grandis,J.R., Gutkind,J.S.,
            Ideker,T. and Krogan,N.J.
  TITLE     A protein network map of head and neck cancer reveals PIK3CA mutant
            drug sensitivity
  JOURNAL   Science 374 (6563), eabf2911 (2021)
   PUBMED   34591642
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5631)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5631)
  AUTHORS   Mena,E.L., Kjolby,R.A.S., Saxton,R.A., Werner,A., Lew,B.G.,
            Boyle,J.M., Harland,R. and Rape,M.
  TITLE     Dimerization quality control ensures neuronal development and
            survival
  JOURNAL   Science 362 (6411) (2018)
   PUBMED   30190310
REFERENCE   5  (bases 1 to 5631)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   6  (bases 1 to 5631)
  AUTHORS   Bennett,E.J., Rush,J., Gygi,S.P. and Harper,J.W.
  TITLE     Dynamics of cullin-RING ubiquitin ligase network revealed by
            systematic quantitative proteomics
  JOURNAL   Cell 143 (6), 951-965 (2010)
   PUBMED   21145461
REFERENCE   7  (bases 1 to 5631)
  AUTHORS   Flachsbart,F., Franke,A., Kleindorp,R., Caliebe,A., Blanche,H.,
            Schreiber,S. and Nebel,A.
  TITLE     Investigation of genetic susceptibility factors for human longevity
            - a targeted nonsynonymous SNP study
  JOURNAL   Mutat Res 694 (1-2), 13-19 (2010)
   PUBMED   20800603
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 5631)
  AUTHORS   Teslovich,T.M., Musunuru,K., Smith,A.V., Edmondson,A.C.,
            Stylianou,I.M., Koseki,M., Pirruccello,J.P., Ripatti,S.,
            Chasman,D.I., Willer,C.J., Johansen,C.T., Fouchier,S.W., Isaacs,A.,
            Peloso,G.M., Barbalic,M., Ricketts,S.L., Bis,J.C., Aulchenko,Y.S.,
            Thorleifsson,G., Feitosa,M.F., Chambers,J., Orho-Melander,M.,
            Melander,O., Johnson,T., Li,X., Guo,X., Li,M., Shin Cho,Y., Jin
            Go,M., Jin Kim,Y., Lee,J.Y., Park,T., Kim,K., Sim,X., Twee-Hee
            Ong,R., Croteau-Chonka,D.C., Lange,L.A., Smith,J.D., Song,K., Hua
            Zhao,J., Yuan,X., Luan,J., Lamina,C., Ziegler,A., Zhang,W.,
            Zee,R.Y., Wright,A.F., Witteman,J.C., Wilson,J.F., Willemsen,G.,
            Wichmann,H.E., Whitfield,J.B., Waterworth,D.M., Wareham,N.J.,
            Waeber,G., Vollenweider,P., Voight,B.F., Vitart,V.,
            Uitterlinden,A.G., Uda,M., Tuomilehto,J., Thompson,J.R., Tanaka,T.,
            Surakka,I., Stringham,H.M., Spector,T.D., Soranzo,N., Smit,J.H.,
            Sinisalo,J., Silander,K., Sijbrands,E.J., Scuteri,A., Scott,J.,
            Schlessinger,D., Sanna,S., Salomaa,V., Saharinen,J., Sabatti,C.,
            Ruokonen,A., Rudan,I., Rose,L.M., Roberts,R., Rieder,M.,
            Psaty,B.M., Pramstaller,P.P., Pichler,I., Perola,M., Penninx,B.W.,
            Pedersen,N.L., Pattaro,C., Parker,A.N., Pare,G., Oostra,B.A.,
            O'Donnell,C.J., Nieminen,M.S., Nickerson,D.A., Montgomery,G.W.,
            Meitinger,T., McPherson,R., McCarthy,M.I., McArdle,W., Masson,D.,
            Martin,N.G., Marroni,F., Mangino,M., Magnusson,P.K., Lucas,G.,
            Luben,R., Loos,R.J., Lokki,M.L., Lettre,G., Langenberg,C.,
            Launer,L.J., Lakatta,E.G., Laaksonen,R., Kyvik,K.O., Kronenberg,F.,
            Konig,I.R., Khaw,K.T., Kaprio,J., Kaplan,L.M., Johansson,A.,
            Jarvelin,M.R., Janssens,A.C., Ingelsson,E., Igl,W., Kees
            Hovingh,G., Hottenga,J.J., Hofman,A., Hicks,A.A., Hengstenberg,C.,
            Heid,I.M., Hayward,C., Havulinna,A.S., Hastie,N.D., Harris,T.B.,
            Haritunians,T., Hall,A.S., Gyllensten,U., Guiducci,C., Groop,L.C.,
            Gonzalez,E., Gieger,C., Freimer,N.B., Ferrucci,L., Erdmann,J.,
            Elliott,P., Ejebe,K.G., Doring,A., Dominiczak,A.F., Demissie,S.,
            Deloukas,P., de Geus,E.J., de Faire,U., Crawford,G., Collins,F.S.,
            Chen,Y.D., Caulfield,M.J., Campbell,H., Burtt,N.P.,
            Bonnycastle,L.L., Boomsma,D.I., Boekholdt,S.M., Bergman,R.N.,
            Barroso,I., Bandinelli,S., Ballantyne,C.M., Assimes,T.L.,
            Quertermous,T., Altshuler,D., Seielstad,M., Wong,T.Y., Tai,E.S.,
            Feranil,A.B., Kuzawa,C.W., Adair,L.S., Taylor,H.A. Jr.,
            Borecki,I.B., Gabriel,S.B., Wilson,J.G., Holm,H.,
            Thorsteinsdottir,U., Gudnason,V., Krauss,R.M., Mohlke,K.L.,
            Ordovas,J.M., Munroe,P.B., Kooner,J.S., Tall,A.R., Hegele,R.A.,
            Kastelein,J.J., Schadt,E.E., Rotter,J.I., Boerwinkle,E.,
            Strachan,D.P., Mooser,V., Stefansson,K., Reilly,M.P., Samani,N.J.,
            Schunkert,H., Cupples,L.A., Sandhu,M.S., Ridker,P.M., Rader,D.J.,
            van Duijn,C.M., Peltonen,L., Abecasis,G.R., Boehnke,M. and
            Kathiresan,S.
  TITLE     Biological, clinical and population relevance of 95 loci for blood
            lipids
  JOURNAL   Nature 466 (7307), 707-713 (2010)
   PUBMED   20686565
REFERENCE   9  (bases 1 to 5631)
  AUTHORS   Nam,S., Min,K., Hwang,H., Lee,H.O., Lee,J.H., Yoon,J., Lee,H.,
            Park,S. and Lee,J.
  TITLE     Control of rapsyn stability by the CUL-3-containing E3 ligase
            complex
  JOURNAL   J Biol Chem 284 (12), 8195-8206 (2009)
   PUBMED   19158078
REFERENCE   10 (bases 1 to 5631)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092658.2, CN290430.1, BX640727.1, BX647454.1, BX538173.1,
            BX640744.1 and AA878129.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020803.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.259598.1,
                                           SRR1660803.9680.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000273963.10/ ENSP00000273963.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC092658.2         125551-125560       c
            11-101              CN290430.1         16-106
            102-3778            BX640727.1         2-3678
            3779-4689           BX647454.1         3705-4615
            4690-4708           BX538173.1         4597-4615
            4709-5104           BX640744.1         4397-4792
            5105-5105           AC092658.2         65571-65571         c
            5106-5485           BX640744.1         4794-5173
            5486-5631           AA878129.1         17-162              c
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..5631
                     /gene="KLHL8"
                     /note="kelch like family member 8"
                     /db_xref="GeneID:57563"
                     /db_xref="HGNC:HGNC:18644"
                     /db_xref="MIM:611967"
     exon            1..201
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            202..568
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..2215
                     /gene="KLHL8"
                     /note="isoform 1 is encoded by transcript variant 2;
                     kelch-like protein 8; kelch-like 8"
                     /codon_start=1
                     /product="kelch-like protein 8 isoform 1"
                     /protein_id="NP_065854.3"
                     /db_xref="CCDS:CCDS3617.1"
                     /db_xref="GeneID:57563"
                     /db_xref="HGNC:HGNC:18644"
                     /db_xref="MIM:611967"
                     /translation="MASDSMSSKQARNHITKGKRQQQHQQIKNRSSISDGDGEDSFIF
                     EANEAWKDFHGSLLRFYENGELCDVTLKVGSKLISCHKLVLACVIPYFRAMFLSEMAE
                     AKQTLIEIRDFDGDAIEDLVKFVYSSRLTLTVDNVQPLLYAACILQVELVARACCEYM
                     KLHFHPSNCLAVRAFAESHNRIDLMDMADQYACDHFTEVVECEDFVSVSPQHLHKLLS
                     SSDLNIENEKQVYNAAIKWLLANPQHHSKWLDETLAQVRLPLLPVDFLMGVVAKEQIV
                     KQNLKCRDLLDEARNYHLHLSSRAVPDFEYSIRTTPRKHTAGVLFCVGGRGGSGDPFR
                     SIECYSINKNSWFFGPEMNSRRRHVGVISVEGKVYAVGGHDGNEHLGSMEMFDPLTNK
                     WMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCFNDVERYDIESDQWSTVAPMNTPR
                     GGVGSVALVNHVYAVGGNDGMASLSSVERYDPHLDKWIEVKEMGQRRAGNGVSKLHGC
                     LYVVGGFDDNSPLSSVERYDPRSNKWDYVAALTTPRGGVGIATVMGKIFAVGGHNGNA
                     YLNTVEAFDPVLNRWELVGSVSHCRAGAGVAVCSCLTSQIRDVGHGSNNVVDCM"
     exon            569..1117
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1304
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1448
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1560
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1729
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1889
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..2091
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..5631
                     /gene="KLHL8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5141..5146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL8"
                     /note="hexamer: AATAAA"
     polyA_site      5163
                     /gene="KLHL8"
                     /note="major polyA site"
     regulatory      5251..5256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL8"
                     /note="hexamer: CATAAA"
     polyA_site      5276
                     /gene="KLHL8"
     regulatory      5455..5460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL8"
                     /note="hexamer: AGTAAA"
     polyA_site      5485
                     /gene="KLHL8"
     regulatory      5598..5603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL8"
                     /note="hexamer: ATTAAA"
     regulatory      5603..5608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL8"
                     /note="hexamer: AATACA"
     polyA_site      5627
                     /gene="KLHL8"
     polyA_site      5631
                     /gene="KLHL8"
ORIGIN      
        1 agaggccgcg gctcgcgggg gctgaggcga gaggacgcca agcgcccgcc ggggctccgc
       61 ggggccgcgc aggagagcgc gcgtccgccg ggtgctcgcg cggggttggt gaggttccgc
      121 tgagggcggc cggggctctg ggagcgtggt aacgtggctg caaccttagc gacatcagga
      181 agaacagggt cgaggatcga gtcacagcaa actgtacaat ggcagttgag tgggttttga
      241 aaaacgtctg aattgtagtg tctacatcta ccttctgcct tctctctacc ctttgaaaaa
      301 aaataaatgg catgtttgag aagagagctc tattaaaaga ggaatattgc aaatggcttc
      361 agattctatg agtagtaaac aagctaggaa tcacattaca aaggggaaaa ggcaacaaca
      421 gcaccagcaa ataaagaaca gatcctcaat tagtgatggt gatggagaag attcctttat
      481 ttttgaagca aatgaagctt ggaaagattt tcatggttct cttcttcgat tttatgaaaa
      541 tggagaactc tgtgatgtca cactcaaggt tggctcaaag ctaatctctt gtcacaagct
      601 ggtattggct tgtgttattc cctactttag agccatgttt ctttctgaaa tggctgaagc
      661 caagcaaacg ctgattgaga ttagagattt tgatggtgat gcaatagaag acttggtaaa
      721 gtttgtctat tcttcacggc tcactttgac tgttgacaat gtccagcctc tcttatatgc
      781 agcctgtatt ctgcaggttg aactggtggc tagagcttgt tgtgaataca tgaagttaca
      841 ttttcatccc tccaattgcc tggcagtaag agcctttgca gaaagtcaca atcgaataga
      901 cttaatggac atggcggatc agtatgcctg tgaccatttt actgaagtag tggagtgtga
      961 agactttgta agtgtatcac cgcagcacct ccataagctt ttgtcctcca gtgatctaaa
     1021 tattgaaaat gaaaagcagg tctataatgc tgccatcaag tggcttcttg ccaatcctca
     1081 gcatcattcc aaatggttgg atgaaacact tgcacaggtt cgcctgccat tgttgccggt
     1141 tgattttctt atgggtgttg tggcaaaaga acagattgtc aagcaaaatc taaaatgtag
     1201 agatttactg gatgaagcaa gaaattacca ccttcacttg agtagcagag cagtacctga
     1261 ctttgaatac tccattcgga ctaccccaag gaagcatact gctggtgtgc tgttttgtgt
     1321 aggtggtcga ggtggatctg gtgacccctt tcgcagtatt gaatgctatt ctatcaacaa
     1381 aaacagttgg ttctttggac cagaaatgaa tagtcgaagg cgacatgtgg gtgtaatctc
     1441 tgtggaaggt aaagtgtatg cagtaggtgg acatgatgga aatgaacatt tagggagtat
     1501 ggagatgttt gatcctctca ctaataaatg gatgatgaag gcatcaatga acacaaagag
     1561 gcgaggaatt gccttggctt ccttaggagg cccaatttat gcaattggag ggttagatga
     1621 caatacttgc ttcaatgatg tggagagata tgacatagaa tctgatcagt ggagtacagt
     1681 ggcaccaatg aatactcccc gtggaggagt tggctctgtt gctctagtaa accatgttta
     1741 tgcagtaggt ggcaatgatg gaatggcttc tttatctagc gtggagagat atgatccaca
     1801 tctggataag tggatagaag ttaaagaaat gggtcagcga agagcaggca atggagttag
     1861 caagcttcat ggttgcttat acgtagttgg tggttttgat gataattctc ctctgagttc
     1921 agttgagcgg tatgaccccc gaagcaacaa gtgggattat gtggcagcac ttactactcc
     1981 cagaggtgga gtgggaatcg caacagtgat gggcaaaatc tttgcagttg gtggtcataa
     2041 tggcaatgca tacttaaata cagtagaagc gtttgatcca gtgctgaata ggtgggagct
     2101 tgttggatct gtgtctcact gcagagctgg agcaggagta gctgtgtgtt cctgtttaac
     2161 tagccaaatt cgagatgtag gtcatggatc caataatgtg gttgactgta tgtgatttga
     2221 gttctggcca ccaacaattt agtcatatct gataggtaca aaagaaaacc aagattttga
     2281 tatgaccacc tttcaacact ttttactgca actagagtct tatttaaatg ttaactgttg
     2341 tattgtgact aagtacaact tttggaatga tacctgaaga attattagta atagagttaa
     2401 atttgatact tcctgctttt gcaaataatg gagtagggaa gaagatagtc gaacttaaaa
     2461 tcatacctac cctgaaaaat gcttaaagta gtgccaaaaa tcttgaaatc tctctctctc
     2521 tctctatttg gatgaatcaa aatgtaattt gttgagtttt gccttttctt ttgcctaaag
     2581 atgttaacgt caggcagtgg gtttttccta gaggagagat tagtacctat tcattaacac
     2641 aaggatacat aagagacttg ccccttttga agatcatttc tacttggtgc tgatgaattt
     2701 atgcctagct ttttttttct aggtggtgat ttttcaattt cttttatatt tacggaagta
     2761 gaaaaaatgt tctgtctttt tgaatgccac ttagctgcat tttcagaagc ttataggcta
     2821 taagaagcct ttaatttcag tttgaattgc taggacacca aaagacctag ctgggctaac
     2881 tccatccaaa tgattttaca gttttatttt ttcatctttt gcaaaaagta tttgttacat
     2941 aatcaaaagg tgaaccagag gaaatattga acaaggtttt agatctgatg ttattctggt
     3001 gcccaattga gagaccttga gaaagaatga tgcagattac atgactctaa tgtgggcagt
     3061 ctgaaaatat ctttaactgt cttctttgat catttgattt tggatccaag atttatcatc
     3121 tctctatttt gctatttgcc accatcctgg tttttctgac tgtacagaat caatctgcat
     3181 ttatttatta tcagaaatga atgcattttt acaaaaacct ttttgtagac tgtagtttat
     3241 tgattagttc aagcccatca cgaactaatt aaacatattg ttggtaaaaa cagagattga
     3301 aattactgtt tttatcatat ttaaatccct ctggtgtatt tattgaaatt taattaaaaa
     3361 tatttctaca tattgatttg aagccaggat attgatggac ctacagattt aacagctctt
     3421 caaagtttta actagcatta taaaatggcc tattcattat atgtatattt ttttataaat
     3481 atttatcaag aacatgaaaa tattttattt tgtatttgca gtgcagtttg gcacctctct
     3541 tccaggcctt cctaagtttt tgttagttgt gtcttcgaat ttattgtagt tcaccgctgg
     3601 tcagtaatct ggttcataag gaagctgggg cagtgaaaaa cacttcgttg atccatccag
     3661 ccctaaagtt cattgactaa tagctcatgc atcttattaa atgggattct ggacttctac
     3721 aatagtgtgg aaagccacat cacaaaaaaa gccacatcac aaaaaaaagc cagtgcctat
     3781 tgagtatatc aaaccaaaaa agaaaaaaaa aagttttaag aggggtcagg ctggcagata
     3841 aaagtctctt atgaagcaga tataaatcca gtaagctatt tttttctacc tatgggcaag
     3901 ttcataagtc attgctggat ctgaaccagt gttcttttgt gtaagtacaa ccatatttca
     3961 gtcagtccag aggcagttaa ccctgtagca cttactagac tggtgacaac tggctggatg
     4021 tggagaataa gtgtcaaaac ctggcttcaa tgtctatttc ttatgtctaa tggagttctt
     4081 atattacagt gtttgtttag ctatggttac taatatgttt gttatattta ttttcgggga
     4141 gaggatcttg ttattgtttc ctgagattat ttaaaatgtt ttttctttga tgtatttctg
     4201 tctttcttat atgtaaggag ttataagggt tattatattt tcatttctgg tttttactat
     4261 acaatggtta tctaacatac taagttggta ccctacctac ccgtaaaata tgaaatactc
     4321 agaagtaaag tgtgtgggca atcagaagca tagccttgaa ggacctgaca gcagtatgaa
     4381 aagataaata ggccaggcat ggtggctcac gcctgtgatc ccagcacttt gggaggccga
     4441 ggtgggtgga ccacctgaga tcaggtgttc aagaccagcc tgaccaacat ggtgaaaccc
     4501 tgtctctact aaaagtacaa aaattagcca ggcgtggtgg cgcatacctg taattccagc
     4561 tactcaggag gctgaggcag gagaattgct tgaacccagg aggtggaggt tgcaatgagc
     4621 cgaggttgag ccatcgcact ccagcggtga aacaagagtg aaactccatc tcaaaaaaaa
     4681 aaaaaaaaaa aagataaaaa agcagaatca atatgtgcac agttctcttt atcaggcaaa
     4741 aacacgttta ttgagacatg aaatgagaat caggctgaag gaatcatgca gctgaaagat
     4801 gattatattc atagaaatga agagatgaaa tattcaaaga agatggctac tttattgtga
     4861 gacttaccac tttaacctca tatgttaaca gcacctacca aaaaatgata tgagatagag
     4921 ctaaaatact gaaattgcaa atggacaaaa taaatcatga aaatttgttt attatttctt
     4981 caataaatat ttctaggtgc tttgtgattc tttcatttag tcattaaagc cattgcttta
     5041 ctatattgcg ttgccacttt aaaaacaagt tacttgacat tgtttttgac agatttcact
     5101 atatacttat gtaattaatt gattatttct aaaatgtttg aataaacttt gtactccctc
     5161 taaggagtat tttattcatt cttttaattc ccaaatgaac tttgaactta cagtgctgtt
     5221 gaatgccttg gtaaggactt cttgaaattg cataaactat tcttgtaaat ggtaaacttg
     5281 agaattttct ttgtttcttt tttctctatg tcttaaagaa tgcctttaga atctaattca
     5341 tgttgtatca gttaagaatg tttctgtggt ttctcgtatc agatgtgtaa agaaattatt
     5401 actgggatgc cattggctat gtctctttcc ccttcatgta aaattccaag tgtcagtaaa
     5461 acttctctat aatattttct taaaaccatt tataaatttg tgttataata ctccagaaaa
     5521 tcaatgtaat aagtggtcat atatatcacc aactatggat atcaaaaagt tggggtcaca
     5581 tcagtgctat ttatttaatt aaaatacatt gcatatttct attagcacaa a
//
