LOCUS       NM_017415               6805 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens kelch like family member 3 (KLHL3), transcript variant
            1, mRNA.
ACCESSION   NM_017415
VERSION     NM_017415.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ying,S., Guo,Q. and Zhang,C.
  TITLE     KLHL3-dependent WNK4 degradation affected by potassium through the
            neddylation and autophagy pathway
  JOURNAL   BMC Nephrol 24 (1), 217 (2023)
   PUBMED   37481568
  REMARK    GeneRIF: KLHL3-dependent WNK4 degradation affected by potassium
            through the neddylation and autophagy pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Lin,Y., Li,Q. and Jin,X.
  TITLE     Kelch-like protein 3 in human disease and therapy
  JOURNAL   Mol Biol Rep 49 (10), 9813-9824 (2022)
   PUBMED   35585379
  REMARK    GeneRIF: Kelch-like protein 3 in human disease and therapy.
            Review article
REFERENCE   3  (bases 1 to 6805)
  AUTHORS   Chen,Z., Zhang,J., Murillo-de-Ozores,A.R., Castaneda-Bueno,M.,
            D'Amico,F., Heilig,R., Manning,C.E., Sorrell,F.J., D'Angiolella,V.,
            Fischer,R., Mulder,M.P.C., Gamba,G., Alessi,D.R. and Bullock,A.N.
  TITLE     Sequence and structural variations determining the recruitment of
            WNK kinases to the KLHL3 E3 ligase
  JOURNAL   Biochem J 479 (5), 661-675 (2022)
   PUBMED   35179207
REFERENCE   4  (bases 1 to 6805)
  AUTHORS   Etges,A., Hellmig,N., Walenda,G., Haddad,B.G., Machtens,J.P.,
            Morosan,T., Rump,L.C. and Scholl,U.I.
  TITLE     A Novel Homozygous KLHL3 Mutation as a Cause of Autosomal Recessive
            Pseudohypoaldosteronism Type II Diagnosed Late in Life
  JOURNAL   Nephron 146 (4), 418-428 (2022)
   PUBMED   35093948
REFERENCE   5  (bases 1 to 6805)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 6805)
  AUTHORS   Louis-Dit-Picard,H., Barc,J., Trujillano,D., Miserey-Lenkei,S.,
            Bouatia-Naji,N., Pylypenko,O., Beaurain,G., Bonnefond,A., Sand,O.,
            Simian,C., Vidal-Petiot,E., Soukaseum,C., Mandet,C., Broux,F.,
            Chabre,O., Delahousse,M., Esnault,V., Fiquet,B., Houillier,P.,
            Bagnis,C.I., Koenig,J., Konrad,M., Landais,P., Mourani,C.,
            Niaudet,P., Probst,V., Thauvin,C., Unwin,R.J., Soroka,S.D.,
            Ehret,G., Ossowski,S., Caulfield,M., Bruneval,P., Estivill,X.,
            Froguel,P., Hadchouel,J., Schott,J.J. and Jeunemaitre,X.
  CONSRTM   International Consortium for Blood Pressure (ICBP)
  TITLE     KLHL3 mutations cause familial hyperkalemic hypertension by
            impairing ion transport in the distal nephron
  JOURNAL   Nat Genet 44 (4), 456-460,S1-S3 (2012)
   PUBMED   22406640
  REMARK    GeneRIF: identified KLHL3 as a third gene responsible for familial
            hyperkalemic hypertension; study establishes a role for KLHL3 as a
            new member of the complex signaling pathway regulating ion
            homeostasis in the distal nephron and indirectly blood pressure
            Erratum:[Nat Genet. 2012;44(5):609]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6805)
  AUTHORS   Boyden,L.M., Choi,M., Choate,K.A., Nelson-Williams,C.J., Farhi,A.,
            Toka,H.R., Tikhonova,I.R., Bjornson,R., Mane,S.M., Colussi,G.,
            Lebel,M., Gordon,R.D., Semmekrot,B.A., Poujol,A., Valimaki,M.J., De
            Ferrari,M.E., Sanjad,S.A., Gutkin,M., Karet,F.E., Tucci,J.R.,
            Stockigt,J.R., Keppler-Noreuil,K.M., Porter,C.C., Anand,S.K.,
            Whiteford,M.L., Davis,I.D., Dewar,S.B., Bettinelli,A.,
            Fadrowski,J.J., Belsha,C.W., Hunley,T.E., Nelson,R.D.,
            Trachtman,H., Cole,T.R., Pinsk,M., Bockenhauer,D., Shenoy,M.,
            Vaidyanathan,P., Foreman,J.W., Rasoulpour,M., Thameem,F.,
            Al-Shahrouri,H.Z., Radhakrishnan,J., Gharavi,A.G., Goilav,B. and
            Lifton,R.P.
  TITLE     Mutations in kelch-like 3 and cullin 3 cause hypertension and
            electrolyte abnormalities
  JOURNAL   Nature 482 (7383), 98-102 (2012)
   PUBMED   22266938
  REMARK    GeneRIF: fundamental role for KLHL3 and CUL3 in blood pressure,
            K(+) and pH homeostasis
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6805)
  AUTHORS   Furukawa,M., He,Y.J., Borchers,C. and Xiong,Y.
  TITLE     Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin
            ligases
  JOURNAL   Nat Cell Biol 5 (11), 1001-1007 (2003)
   PUBMED   14528312
REFERENCE   9  (bases 1 to 6805)
  AUTHORS   Lai,F., Orelli,B.J., Till,B.G., Godley,L.A., Fernald,A.A.,
            Pamintuan,L. and Le Beau,M.M.
  TITLE     Molecular characterization of KLHL3, a human homologue of the
            Drosophila kelch gene
  JOURNAL   Genomics 66 (1), 65-75 (2000)
   PUBMED   10843806
REFERENCE   10 (bases 1 to 6805)
  AUTHORS   Ellison,D.H.
  TITLE     Pseudohypoaldosteronism Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22073419
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA083147.1, AB032955.1 and
            AC106775.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_017415.2.
            
            Summary: This gene is ubiquitously expressed and encodes a
            full-length protein which has an N-terminal BTB domain followed by
            a BACK domain and six kelch-like repeats in the C-terminus. These
            kelch-like repeats promote substrate ubiquitination of bound
            proteins via interaction of the BTB domain with the CUL3 (cullin 3)
            component of a cullin-RING E3 ubiquitin ligase (CRL) complex.
            Muatations in this gene cause pseudohypoaldosteronism type IID
            (PHA2D); a rare Mendelian syndrome featuring hypertension,
            hyperkalaemia and metabolic acidosis. Alternative splicing results
            in multiple transcript variants encoding distinct isoforms.
            [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1; also known as KLHL3a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF208068.1, SRR1660805.258662.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309755.9/ ENSP00000312397.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-229               DA083147.1         2-230
            230-2525            AB032955.1         1-2296
            2526-6805           AC106775.3         38162-42441
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.2"
     gene            1..6805
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /note="kelch like family member 3"
                     /db_xref="GeneID:26249"
                     /db_xref="HGNC:HGNC:6354"
                     /db_xref="MIM:605775"
     exon            1..457
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     CDS             444..2207
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kelch-like protein 3"
                     /codon_start=1
                     /product="kelch-like protein 3 isoform 1"
                     /protein_id="NP_059111.2"
                     /db_xref="CCDS:CCDS4192.1"
                     /db_xref="GeneID:26249"
                     /db_xref="HGNC:HGNC:6354"
                     /db_xref="MIM:605775"
                     /translation="MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELR
                     SKQLLCDVMIVAEDVEIEAHRVVLAACSPYFCAMFTGDMSESKAKKIEIKDVDGQTLS
                     KLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCCDFLQSQLHPTNCLGIRAFAD
                     VHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSEEKVFEAVI
                     SWINYEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYH
                     LLPLDQRLLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELP
                     SRRCRAGVVFMAGHVYAVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVL
                     NDLLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVVEGKLYAVGGYD
                     GASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSGQLYATGGHDGPLVRKSVE
                     VYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPVTDKWTL
                     LPTNMSTGRSYAGVAVIHKSL"
     exon            458..577
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            578..684
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            685..806
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            807..969
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1079
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1196
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1346
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1464
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1662
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1764
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1893
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2034
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2178
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..6805
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6784..6789
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /note="hexamer: AATAAA"
     polyA_site      6805
                     /gene="KLHL3"
                     /gene_synonym="PHA2D"
                     /note="major polyA site"
ORIGIN      
        1 attctttgca gcctagacag aggaggcagg agccccaggg gcgggctaat cgcctgggct
       61 ggggatgcct gggcagatgc agaggaagct ggaaaggtgg cagtgcacct gggtcgctgg
      121 agctgccgcc gttcctagga gaccaaggag cagcaagcct gcgggggagg gggagcaagt
      181 gggttgctgc ttttagcagc tgaaagggct gcagggagct ctgggtaaga cattttctgt
      241 tgctgctgct tttgcggtag aagctgctgc gagtaagtca gaggaaggag gattgagaag
      301 ggaggaggct tcacttgcag ccatgctgaa tcactgttgc tgatcagatt tcccactggt
      361 ctgctgggag aatcaagaac agaactagga tccccgagtg catacacagt tcagcagcta
      421 ccaccctggc tgggcctcac acaatggagg gtgaaagtgt caagctgagc tcccagactc
      481 tgatacaggc tggggatgat gagaagaacc agaggacgat cactgtcaac cctgcccaca
      541 tggggaaagc attcaaggtt atgaatgaac tgcggagtaa acagctgttg tgtgacgtga
      601 tgattgtggc agaagatgtc gagatagaag cccaccgtgt ggtcctggca gcctgcagcc
      661 cctacttctg tgcgatgttc acaggtgaca tgtctgagag taaagccaaa aagatagaaa
      721 tcaaggacgt ggatgggcag acgctgagta agctgattga ctacatctat actgctgaaa
      781 tcgaggtgac tgaagagaat gtccaggtgc tgctcccggc agccagcttg ctgcagctca
      841 tggatgttcg gcagaactgc tgtgacttcc tgcagtctca gttgcatccc accaattgcc
      901 tgggcatccg tgcatttgca gatgtacaca cctgcactga ccttctgcag caggccaatg
      961 cctacgcaga gcagcacttt ccagaggtga tgctaggaga agaatttctt agcctgagtc
     1021 tggaccaggt gtgcagcttg atatccagcg acaagctgac cgtttcttca gaagagaagg
     1081 tgtttgaagc tgtgatctca tggatcaatt atgagaaaga aacccgttta gagcacatgg
     1141 caaagctgat ggaacatgtc cgacttcctc tcttacctag ggactaccta gtccaaacgg
     1201 ttgaagaaga agctttgata aagaataaca acacctgtaa agacttcctc attgaggcca
     1261 tgaaatacca tctcctccct ctggatcaga gactattgat taagaaccca aggaccaagc
     1321 ccaggactcc agtcagcctt cccaaggtca tgattgtggt tggcggccag gcacccaagg
     1381 caatccgcag tgtggagtgc tatgatttcg aggaggaccg gtgggatcag attgctgagc
     1441 ttccttccag aagatgcaga gcaggtgtgg tgttcatggc tggccacgtg tatgccgtgg
     1501 gagggtttaa tggctcactg cgggtgcgga cagtggatgt gtatgacggc gtgaaggacc
     1561 agtggacgtc cattgccagc atgcaggagc gccggagcac actgggcgca gcggtgctca
     1621 atgacttgct ctacgcagtg ggaggctttg atggcagtac tggcctagca tcggtggaag
     1681 cctacagcta caagaccaac gagtggttct ttgtggcccc gatgaacacg cggcggagca
     1741 gtgtgggtgt gggcgttgtg gaggggaagc tatatgctgt tgggggttat gatggagctt
     1801 cccgccagtg tctgagcact gtggagcagt acaacccagc gaccaatgaa tggatatacg
     1861 tggcggacat gagcacccgc cgcagtggcg caggggttgg agtgcttagc ggacagctgt
     1921 acgccacagg tgggcatgat gggcctttgg tgaggaagag cgttgaggtt tacgatcctg
     1981 gaacaaatac ctggaagcaa gtggcagaca tgaacatgtg ccggcgcaac gcaggggtct
     2041 gtgcagtaaa tgggctcctg tatgtggttg gaggggatga tggatcctgc aacttggctt
     2101 cggtggagta ctacaatcct gtcactgaca aatggacgct gcttccaacg aacatgagca
     2161 cggggcggag ctatgcaggt gttgccgtga ttcacaagtc cttgtgaccc aaactcctac
     2221 tgccaggagg tggaggaagg agcaggtgct gcctgtgact ctgaacagca ggaccttggt
     2281 gactggattc aacttgcttg ggagggtctg tgctgctgtg agaaccgctc tcctctgact
     2341 tggcagactg gtgttgttca tcgcagtgtg gacaccatta cccacccccg ttcccctgag
     2401 gtgctctggc ctatgccctg agcaaggggg gtcttgacat ccccaggcag cacctttggg
     2461 ctttgttttg gtgtttctac agggacaata cagaccctgg agtgtgtgtg tgtgtgtgtg
     2521 tgtgtgtaga ccatggtgtt tctctatgtt tctctaagtt ggggggtgag cgtgtgtgac
     2581 agtctactgg atttctttac tactgatcct ttcgctgtgt taaaaatcaa gtcacagaga
     2641 cctctcttct ggatttgtcc catggggacc ctgagactac taaagctgct ttcttctgaa
     2701 ggtccagttg gacagtctgg gaatgtccag aaataaccag tgagaggggc agttctctgg
     2761 ccacacccac ttatgtactt taactactgt gactttgtct gcagaagagc tggaaaattc
     2821 tcgaagctgc accgtgtcct ctgtgtgcta gaataaggga caaatgggtt ccctgtgctt
     2881 ctcagctcac tgtttttcct tgagttctcc tacaggaagc agatgagaac tgcccagtct
     2941 tcaggtttag gccattggtc tttgatgtca tagattccag gcctgggagg tgttatgtct
     3001 cttcagctgg gaaaactagc tcttcagaga agcctcgggt aacactgaaa aacaaaacaa
     3061 aacaaaacaa aaacaggaaa aaaacaaaaa accaaagtgg taaggattca gttcctgcct
     3121 ataatggtct cagagagggt cctactttta ggttttccca ggacaggaca gtccccattt
     3181 atacttatta tcccagttta attattcaca gcaccccatt ttactcagaa gtgttctggt
     3241 ctggaggata aataagaggt caccctcctc cagacccaaa gatagatttg tgcctgtgtt
     3301 ggatggggtc gtgtgtgatt cagatggaca ttggatggct tcaaaggaat ataccactag
     3361 agctggccct tggcactttg tgacagtggt caagtctgtc taatgtcctt gtcttctttt
     3421 tcttgtgctt tccccctatt ccagggtgtg caccctctcc ccaaccccca agaaccccac
     3481 tactgctttc cctgtgaggt aggagatatc agtgggcctt ggatttgagg cttcctaaga
     3541 tgtgcttgca ttttaaaaag ggagcttggt gagagctttg ctaattcaca ggtaaaaatt
     3601 attaacaata gaacttcaag catcttgagg agcgggcatt tgagggggca tggagtaatt
     3661 tgtatttaaa aaaccttaaa gttgtgctgt tcctaaacta gcaaattgct catgctgaaa
     3721 tttctggcat aagcagggga agtcttgtgt ctggagaata gtctcatacc ttgcagtctg
     3781 ggacaccctc cctactttga gaatccacct acaggaagcc aaggaacttt ataaatcctg
     3841 atgttggact tctgatacga ctgggctact tccaagcagg tgctgcagga gattggcatc
     3901 ccccagcccc tgcagttaga aaccccgaag tcttcccagc cagtgagcca ctttgtgtat
     3961 ttactgtata tttattgtgc cctaaatgtg caactctcct aaagacaaaa cttctctttc
     4021 tgatgttaag cacatgttac ttcaacaaga tgcttggaga acaacaaggt acccagaatt
     4081 tttagaagcc ttcagaagag gctaaaatat ccagctttgg gggacctgga agaaatgtct
     4141 ccaaaggaag caaggcatgt tttagttgag tgctctggtc tcactatgaa gtggggatga
     4201 ctgtggcttc ataactctac ctggctgtgg gttggaagct gatggaatga gaaatgtcct
     4261 ttctccttct ctgaggaaat tttgagactt gtttcggtgt gtctgtgtga tggggatgag
     4321 gctggggttg ggatctgatg tatgccattc acagaagctc tcaatttcag atgataggtg
     4381 aattccctgc ccctccccca ccactgagaa gctagacttt catgcgggag aggctacttt
     4441 tatgtgtcgt cttccgggga agggtccctc cactgaaagc tagccagtca tgttttctgt
     4501 ttttggattt ttgcaattgg tttcacctca tgtctccctc cctacaaagc actgcctcta
     4561 ctgggcgtgc tgccaaggcc atgtgcactc catcctcatg tatccttttt cacggggacc
     4621 agaacactgg tacgtcatca ccaaagccaa tctgctctag ctgcccacag atgccaccaa
     4681 aacctgctat ctcttcatca ccaggtacga ttctctttcc acagtggaca cagcaggcta
     4741 ttttctagtt tgtgctggtc acgtggtaga tgaagcctct tactgcccca cttagggtgg
     4801 ccacggctgc ttgtgaatgc agctttgcca gtggcatatc tgtcatctga ttgcggtggt
     4861 gaaatggaat tgaggcccaa ggttagaagc agccgagacg ccacttggat actgatttga
     4921 acaatgtaga agtcagattc tgaattccaa agttatttct cataagtacc caatggcatc
     4981 tctccatcta caaagttgca gtattatgca aataaaactg acctcatttt ctgctatgca
     5041 ataagaatac ttaattctag ttcccgacaa gccagttgca atatccccta agatgctttt
     5101 tgagctgtct tactttgata tctgttgtgt aatgtttgta tatttctgag ccagatcctt
     5161 tcaaagattg cctttttata aaattgaagc tatagctttt aggctaaaat tttaacgtag
     5221 atatttttat aagatatttt tttcaagagt ttgaatcgct ttttattgtc catgataatg
     5281 aaatgttgtg ttctttgcat cattcactct caaacgtagt tcatgcctgt agctctcttc
     5341 cttttgtttc tcacccttca gaaacatatt tttcagtagc tccaggtaga tgagcctttt
     5401 tttttttttt aaaaatacca tattcaaggg agtctgctga attttaaaac gcagtcactg
     5461 gtgtttcttg aattgctagg gactgatgtt atgttcgact cagcacttgc ccgtctgtat
     5521 tgattgtgtc tttttttttt tttttggagt ctgctttctg tgggggtgag gccgggctgt
     5581 ctcgtggtgg ctcccactga cgggcactga gcctggtacc ctgtggcatg gagaagcctc
     5641 agggaaaggc ctgccccccc agcacatact cccatagtgt cctaggtcca gccgaccatt
     5701 ccttattctc ttctatctcc ttgttgatct gaagcttcca atagcttgag gcctttgctg
     5761 ctggatgatg ccctttttgg gagcatcttg tctctaacct ttaaaagagg ggtcaatcct
     5821 catgatccct gtgtgttaag catatgcttt gcaggtgctc acactacact tacaacttgc
     5881 ttcttgagct atgtctctac tccaggctct gttttgtgta tttatctgcc atttgcatca
     5941 tggtttttaa aatttattat tattattatt attgttggga caggtgccat ttaaattgcc
     6001 tccatgctcc ccatttgcac ctagctggat caagttggga ggctgagcaa actcatattc
     6061 cagttagttg gagtttttaa aggctctgtt tgcctggaga agcaaggagg ttagaatgta
     6121 atttttttaa gcgtttgcac tatttagagt cctaagcccc tcatgttcag ctgtgctgtg
     6181 tttctactga ccaagcagga gagccagcag cacttccagc atttgggaat ggaagagatt
     6241 tcttctgtag tggataatta cagcctcata gcccctgtgc agccttcgtc atgggactca
     6301 gtgactcatg gatatagcat cagccatggc aggaatgcac aggactgtgg catttgcagc
     6361 atcaaatcac cctagtgcca tgtttggtta tgagattgta aattattcgc tcccccatcc
     6421 tcccctcccc tcattttcag tggcaataga ggacccttgt tgtacttctt gtttaatttg
     6481 catattatgt gtaaaatgct ttcgttgaaa gaaaactgaa gacactgaat gtgtatgtct
     6541 gtgtgggtgc tctgtccctg tggttgtcat agccagtcag acttgatcac tgacaccccg
     6601 tacaacatat tgcataggta agatcctcga tctggtgttc tctgcgtggc tgttagggac
     6661 tgtatatctt gtaaaagaac acttgtcaca tgcttgatca gttacagcaa tagctgaaga
     6721 aacatttcct caaatgtatt attttaacag gaatcatgtt ctaatttccc atcctttaat
     6781 tttaataaaa gctgaactgt gtgaa
//
