LOCUS       NM_017658               6522 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens kelch like family member 28 (KLHL28), transcript
            variant 2, mRNA.
ACCESSION   NM_017658
VERSION     NM_017658.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Dhanoa,B.S., Cogliati,T., Satish,A.G., Bruford,E.A. and
            Friedman,J.S.
  TITLE     Update on the Kelch-like (KLHL) gene family
  JOURNAL   Hum Genomics 7 (1), 13 (2013)
   PUBMED   23676014
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA247273.1, BC121009.1, AL049870.3 and BM998529.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017658.4.
            
            Transcript Variant: This variant (2) uses an alternate 5'-terminal
            exon, which results in a different 5' UTR and use of a downstream
            start codon compared to variant 1. It encodes isoform 2, which has
            a shorter N-terminus than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.8768.1,
                                           SRR14243140.5117160.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396128.9/ ENSP00000379434.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                DA247273.1         19-77
            60-1908             BC121009.1         1-1849
            1909-6082           AL049870.3         75813-79986         c
            6083-6522           BM998529.1         18-457              c
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.2"
     gene            1..6522
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /note="kelch like family member 28"
                     /db_xref="GeneID:54813"
                     /db_xref="HGNC:HGNC:19741"
     exon            1..102
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1818
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /note="isoform 2 is encoded by transcript variant 2; BTB
                     (POZ) domain containing 5; kelch-like protein 28; BTB/POZ
                     domain-containing protein 5; kelch-like 28"
                     /codon_start=1
                     /product="kelch-like protein 28 isoform 2"
                     /protein_id="NP_060128.2"
                     /db_xref="CCDS:CCDS9680.1"
                     /db_xref="GeneID:54813"
                     /db_xref="HGNC:HGNC:19741"
                     /translation="MDHTSPTYMLANLTHLHSEQLLQGLNLLRQHHELCDIILRVGDV
                     KIHAHKVVLASVSPYFKAMFTGNLSEKENSEVEFQCIDETALQAIVEYAYTGTVFISQ
                     DTVESLLPAANLLQIKLVLKECCAFLESQLDPGNCIGISRFAETYGCRDLYLAATKYI
                     CQNFEAVCQTEEFFELTHADLDEIVSNDCLNVATEETVFYALESWIKYDVQERQKYLA
                     QLLNSVRLPLLSVKFLTRLYEANHLIRDDRTCKHLLNEALKYHFMPEHRLSHQTVLMT
                     RPRCAPKVLCAVGGKSGLFACLDSVEMYFPQNDSWIGLAPLNIPRYEFGICVLDQKVY
                     VIGGIATNVRPGVTIRKHENSVECWNPDTNTWTSLERMNESRSTLGVVVLAGELYALG
                     GYDGQSYLQSVEKYIPKIRKWQPVAPMTTTRSCFAAAVLDGMIYAIGGYGPAHMNSVE
                     RYDPSKDSWEMVASMADKRIHFGVGVMLGFIFVVGGHNGVSHLSSIERYDPHQNQWTV
                     CRPMKEPRTGVGAAVIDNYLYVVGGHSGSSYLNTVQKYDPISDTWLDSAGMIYCRCNF
                     GLTAL"
     exon            103..1001
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1445
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1654
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..6522
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6504..6509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /note="hexamer: AATAAA"
     polyA_site      6522
                     /gene="KLHL28"
                     /gene_synonym="BTBD5"
                     /note="major polyA site"
ORIGIN      
        1 gtaagagtta gggtttgacc ggagctagtg aggatccagc ccaggctcca gttgtccgcg
       61 gttcctcctc ctgtcgctgc ccgtgtgatt actgagactc agatggacca cacatccccg
      121 acctacatgc ttgctaactt aacccacttg cattctgaac aacttctgca gggcttgaat
      181 cttcttcgcc aacatcacga actctgtgac atcattcttc gagtaggtga tgttaaaatt
      241 catgctcaca aagtggtact tgccagcgtc agcccgtatt tcaaagctat gttcactgga
      301 aacctttctg aaaaagagaa cagtgaggtt gagtttcaat gcattgatga aactgctctc
      361 caggccattg tggagtatgc ctatacaggg actgttttta tttctcagga cacagttgaa
      421 tctctcctgc cagcagcaaa cctactccag ataaaacttg tcctgaaaga atgttgtgca
      481 tttcttgaaa gccaacttga tcctggtaat tgtattggaa tttctcgttt tgcagaaaca
      541 tatggttgcc gtgaccttta tttggcagcc actaaataca tatgccagaa ttttgaagct
      601 gtttgccaga ctgaagagtt ttttgagctt acacatgctg acttggatga aattgtttcc
      661 aatgactgtt tgaatgtagc taccgaagag actgtttttt atgcattaga gtcttggatc
      721 aagtatgatg tacaagaacg ccagaaatac ttagcacagt tactaaacag tgtacgatta
      781 ccattgttga gtgttaagtt tctcactaga ctatatgaag caaatcatct tattcgtgat
      841 gatcgcactt gtaaacatct tttgaatgaa gccctaaagt accactttat gcctgaacat
      901 agactctctc atcagacagt cttgatgaca cgacctcgct gtgctcccaa agtactttgt
      961 gcagtaggag ggaaatctgg actctttgcc tgtttggata gtgtggagat gtactttcct
     1021 cagaatgact cttggattgg tttggcaccc ctaaacattc ctcgctatga atttggaata
     1081 tgcgttttag accaaaaagt atatgttata ggtggtattg caactaatgt gcgtcctggc
     1141 gtcactatca gaaaacatga aaattcagtg gaatgctgga atcctgatac aaatacttgg
     1201 acttctctag agagaatgaa tgaaagccga agtactcttg gagtagtagt acttgcagga
     1261 gaactttatg ccttaggtgg ttatgatgga caatcttatt tacaatctgt agagaagtac
     1321 attcccaaaa taagaaaatg gcaacctgtg gcaccaatga cgacaacaag aagttgtttt
     1381 gctgcagcgg tattggatgg aatgatatat gccattggtg ggtatggtcc tgcccacatg
     1441 aacagtgtgg agcgttatga tccaagtaag gactcctggg agatggttgc atccatggca
     1501 gataaaagga ttcactttgg cgtgggtgtc atgctaggct ttatttttgt ggtgggtgga
     1561 cataatggag tctcacattt gtccagcatt gaaagatacg atcctcatca aaatcagtgg
     1621 actgtgtgta gaccaatgaa agaacctaga acaggagttg gtgctgctgt aatcgataac
     1681 tacctttatg tcgtcggtgg tcactcaggg tcttcctatc tgaatacagt gcagaaatat
     1741 gatcctatct cagatacgtg gctggattca gctggcatga tatactgtcg ctgcaacttt
     1801 gggttaactg cactttgaca aatgtgaact ctcggaaata gtatggtggt gaaacttgta
     1861 ctgcatgaac atccggatgg cccagttttc tgaaacccac aagctgcatt gctttctttt
     1921 taacttgaag tagcatgaag gctcaaaagt tttgttgggt acttttaatt gagaagtagt
     1981 tttggttgct cttgattaca cagtaaatca ataatcaaaa agaaaaaaga gaaaaaaaaa
     2041 aagtaaagac tttgccaatt gaattctgca ctttttcccc caagactgag atgtactagt
     2101 tttatgttca taagtgtaca tgtagacaaa acgtggctgc tttttttttt tttttttttt
     2161 tattttagct ctccttccct gataaccatg attttctagg atgagcgatg ctttaggtgc
     2221 tggagtgtat tgaatgaatg gacagagtgc tgatactgct tcttggatgt aaatgcttca
     2281 catttttcat ttgttttaaa acaaacaaag ggctgctttt tattttggta cttttaaagg
     2341 ttgtaaataa catgccaata ggtagtcatc tgaatacttt cctgctaaat ggttttaggg
     2401 ctacggatgt aataaatttt taagacaatt gcttttatta tcatgatatt tggggcccta
     2461 ttttaggaaa tcagatattt actgtttatg ccttttgtat atgggcaggt taacgttgga
     2521 tgaatttaat tacctatcta tgctatggtt agcatggata ggagtttaag catatcaggt
     2581 gtttcagttt ttcttcagta gtacagcagg cctcccagtg ctgatgtaaa tatttgagca
     2641 gtgtttagta ccattcttag tgcatattaa tttttttata tatccttata attttagttt
     2701 tttaagatct ttgctttttc aaagtgttca gatataaaat ggtgctaact gtaggattta
     2761 taacaaggct ataactgatg tccttttgga tgttcagtat gagatatgca tgacatgttt
     2821 tgatagtctt ttgcattttt aatggcttga ttcatttgtg cttattaaaa atgttagtga
     2881 ttacgccaat gttaacatcc aaaaatcatc agaatggcct gtgttatgct tttcatggtt
     2941 cctgaatagt gatttaaaga aagttacact tttgatcttc tttcttcttg tgctagatat
     3001 tacatgtctt tcttaaaaac agaaatatat gcattaaata taaagcacta ggcagttatg
     3061 cattactttg tacatttctt aggtaactga atattttaag gttattgagg aaaactggaa
     3121 ctcgatgttt acttgagtaa tagttctaag ttgagttcta ctttgattaa agaaatgatc
     3181 ttttaaagca aaattgtttg ctgtagccag tgacagctta tttaaagaaa gtgtttaact
     3241 tatttgattt tagcatgttt tagtaaatgt ctgttagtct taaacacatt cacttgcatt
     3301 ttattattat gtcggtactt gtattttgga actgtagtca tttgattttc ttttccttga
     3361 ttttttttaa acctgtttgc gatctcctta taaagtctct gcaagataat ttttcttaag
     3421 aaaaaaattc atattatatt cttaagtgcc ataatgttct agactattgt taagaagcca
     3481 tttgtaatta taaaacatag gttcatttgt tagtatatat gtttatttct tgaatactat
     3541 agccgggcaa tttacagttc tttgttcttc cataaactgc cagttgtaga tttgaatata
     3601 tactacaaaa ctatgtaaaa tcatttttaa tcataaatgt agtatcatgc tttatgaata
     3661 tttgcacaga ttataaaacc ttgcaagtga tttttagtct cttctaattt agaactcaac
     3721 aggagcaact aataacaatt gatagtctga gtattttcta tgttgcttgt ctgaataaca
     3781 taataatata tagcaataac tttttcattg atttgaataa atctattgca tagaaatagg
     3841 tgcactattg tagttggccc agactttatt taaagaaaag cagtttaaaa tagattcatc
     3901 acatatttag ttttaaatcc ccaattcagt tttctttgtt tatagcaatc aaattattaa
     3961 atatatccta ttatactatt tttaatcccc tattcccaaa agataaggga atttgaaaga
     4021 ctgtggaaaa tgattttagg acgggcgcag tggctcacgc ctgtaatccc agcacttgga
     4081 gaggccaagg cgggtatatc acctgaggtc aggagttcga gaccagcctg gccaacatgg
     4141 tgaaaccccg tctctactaa aaatacaaaa aattagctgg gcattgtggc aggtacctgt
     4201 aatcccagct acttgggagg ctgaggcagg agaatcactt gaacccagga ggcagaggtt
     4261 gcagtgagcc gagcttgcac cattgcactc cagcctgggc aacaagagtg aaactccacc
     4321 tcaaaaaaaa aaaaagattt taattataaa cacatctatt ctatacattg tattttaaca
     4381 atattttact catgtatcat ctccactatc aaattagtgt ataatcattt ggtaaattag
     4441 atcaaaaaca tatactggtc attgtttttg aaaaatctaa atttcatctt gatgaagact
     4501 aaatgtgaaa tatcttgggc agtagtagta ggagccatac accagtgaac aaattatgaa
     4561 aatctgtgaa aggaagagga aaaatgttca aaatttagaa gtatatttat tttaggattg
     4621 cttaacctac tgtattattc gtaacaacca gagctactaa tctgacctga caactaatat
     4681 gtttacatta gcgccttctt ttacaaattt gtgagaagtt tcattatgaa cactctgctg
     4741 acaaattgtg aatttaaagc cacatgtttc tatgttaaaa aggatcaaaa tgtttggtta
     4801 taaatcctca ggattaaaag tattagaaat aattttacat attcaggcaa gaagagaaaa
     4861 gctatttaag atttcccaga aatattttgg aaaagagcag cactgggaaa tagaaattaa
     4921 tgtaatcagt tgcaagatac ttgccttcta taattataac aacttaatct gttaggcacc
     4981 tttggctatt caataatcat tggatgttgt aatagaatgt aagtggcttt taatgtagct
     5041 ccaaggatat ttataaattg tgttattgct gttattgttt taaatgtgag tgaatattaa
     5101 attataggaa gtggtatcaa gtgataggaa aatggcatta ggttttgaaa ttttattttg
     5161 ataatttaac acatatggag ggtttgctat atgtatgaga tattctgtta gatgctgtgg
     5221 gagctagaga gatatactct ttttcactca aagagagtaa aatatagtgg gagagaccct
     5281 aataactatt ttggtgaagg atgaatctaa ttccttatct ctgacatcgt aagaagtgat
     5341 tagagaccta gtttatgcat aagcaattcc ttagtgtcca attaggtttt attggcatga
     5401 aaaagaaata ttaaaaaacc taatatttat agaactccaa atctcctaga attggataat
     5461 gtattttcta gtaagaaaac tatagaaatt ttaaatatat atatttcctg tagtatcaag
     5521 actctggctg acattgagag aaatatatgt acatgttgtg tttattttca gttttggtgt
     5581 tggattcctg gtattgtcac ctaagtgctc attttcagga aaatcagatt ctacaataaa
     5641 cacttactgc ttaaagttca gtaaacttgt gataacctca taacctctgt agtctaaaag
     5701 ttaaggaatt ataagatgtc acatgtcaag ttaacatcca gttctacttc ttaacctttt
     5761 ttccctttgt aaacagcact ttaattagac atgatttaat tagataacaa ttattccatg
     5821 gctggatatg tactggattt tcacttacag tacattgatt tggtgttgat acacatttga
     5881 aaagagtgag gaatacaata catttttaat acaataggtt tacaaatttt cctactggtg
     5941 tgaatatgga gtcaaataaa aatccagttt acatctctgc agagagaaaa attgttatcc
     6001 atatgtcttt tatattatac atctttacta gagataactt attgttaatt ttgtcaagtt
     6061 atgtgtaagt cccatttatt tttaaatact aagcaaaaag ttccaagttt gccttaaaat
     6121 acaagtgaag ttaaagaact ggatgataat gcctatattt ttgccttatg aattgtgctg
     6181 tgtattataa accagcaacg gttttgattt taacaggatt ctgaaatttt gtagctgtag
     6241 agtactaata tttgtactct cttgcaaaga atgtgatcta gttgtggatt atgttagttt
     6301 aaatgttttt gtatatgtat tttacctctg aacatgtacc tttttagcat cagggtttta
     6361 ttttatttat gtttacacac taaagtgtca tttaactagt taagaattgt attctttttt
     6421 attttaaagg ttatttgtgt gtatcattgt ggtaatgttc tgtaattatt atggttaaat
     6481 ttcatttaat gtaaaatgtt taaaataaaa atgaaaaatc ta
//
