LOCUS       NM_014315               4969 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens kelch domain containing 2 (KLHDC2), mRNA.
ACCESSION   NM_014315
VERSION     NM_014315.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Rusnac,D.V., Lin,H.C., Canzani,D., Tien,K.X., Hinds,T.R.,
            Tsue,A.F., Bush,M.F., Yen,H.S. and Zheng,N.
  TITLE     Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin
            Ligase
  JOURNAL   Mol Cell 72 (5), 813-822 (2018)
   PUBMED   30526872
  REMARK    GeneRIF: Three crystal structures have been reported of a CRL2
            substrate receptor, KLHDC2, in complex with the diglycine-ending
            C-end degrons of an early terminated selenoprotein, SelK, and the
            N-terminal proteolytic fragment of USP1.
REFERENCE   3  (bases 1 to 4969)
  AUTHORS   Koren,I., Timms,R.T., Kula,T., Xu,Q., Li,M.Z. and Elledge,S.J.
  TITLE     The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Targeting
            C-Terminal Degrons
  JOURNAL   Cell 173 (7), 1622-1635 (2018)
   PUBMED   29779948
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   Lin,H.C., Yeh,C.W., Chen,Y.F., Lee,T.T., Hsieh,P.Y., Rusnac,D.V.,
            Lin,S.Y., Elledge,S.J., Zheng,N. and Yen,H.S.
  TITLE     C-Terminal End-Directed Protein Elimination by CRL2 Ubiquitin
            Ligases
  JOURNAL   Mol Cell 70 (4), 602-613 (2018)
   PUBMED   29775578
REFERENCE   5  (bases 1 to 4969)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 4969)
  AUTHORS   Lin,H.C., Ho,S.C., Chen,Y.Y., Khoo,K.H., Hsu,P.H. and Yen,H.C.
  TITLE     SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins
            produced by failed UGA/Sec decoding
  JOURNAL   Science 349 (6243), 91-95 (2015)
   PUBMED   26138980
REFERENCE   7  (bases 1 to 4969)
  AUTHORS   Taipale,M., Tucker,G., Peng,J., Krykbaeva,I., Lin,Z.Y., Larsen,B.,
            Choi,H., Berger,B., Gingras,A.C. and Lindquist,S.
  TITLE     A quantitative chaperone interaction network reveals the
            architecture of cellular protein homeostasis pathways
  JOURNAL   Cell 158 (2), 434-448 (2014)
   PUBMED   25036637
REFERENCE   8  (bases 1 to 4969)
  AUTHORS   Chin,K.T., Xu,H.T., Ching,Y.P. and Jin,D.Y.
  TITLE     Differential subcellular localization and activity of kelch repeat
            proteins KLHDC1 and KLHDC2
  JOURNAL   Mol Cell Biochem 296 (1-2), 109-119 (2007)
   PUBMED   16964437
  REMARK    GeneRIF: Data show that KLHDC1 and KLHDC2 have differential
            localization and activity in cultured mammalian cells.
REFERENCE   9  (bases 1 to 4969)
  AUTHORS   Wang,Y., Han,K.J., Pang,X.W., Vaughan,H.A., Qu,W., Dong,X.Y.,
            Peng,J.R., Zhao,H.T., Rui,J.A., Leng,X.S., Cebon,J., Burgess,A.W.
            and Chen,W.F.
  TITLE     Large scale identification of human hepatocellular
            carcinoma-associated antigens by autoantibodies
  JOURNAL   J Immunol 169 (2), 1102-1109 (2002)
   PUBMED   12097419
REFERENCE   10 (bases 1 to 4969)
  AUTHORS   Zhou,H.J., Wong,C.M., Chen,J.H., Qiang,B.Q., Yuan,J.G. and Jin,D.Y.
  TITLE     Inhibition of LZIP-mediated transcription through direct
            interaction with a novel host cell factor-like protein
  JOURNAL   J Biol Chem 276 (31), 28933-28938 (2001)
   PUBMED   11384994
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591767.2 and KF459555.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014315.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.28542.1,
                                           SRR1660803.18680.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298307.10/ ENSP00000298307.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-469               AL591767.2         149494-149962
            470-549             AL591767.2         152935-153014
            550-667             AL591767.2         155902-156019
            668-783             AL591767.2         159180-159295
            784-865             AL591767.2         159519-159600
            866-949             AL591767.2         159752-159835
            950-1030            AL591767.2         160936-161016
            1031-1089           AL591767.2         161089-161147
            1090-1199           AL591767.2         161554-161663
            1200-1272           AL591767.2         162044-162116
            1273-1360           AL591767.2         163711-163798
            1361-1413           AL591767.2         163883-163935
            1414-3043           AL591767.2         164171-165800
            3044-3425           KF459555.1         1-382
            3426-4969           AL591767.2         166183-167726
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.3"
     gene            1..4969
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /note="kelch domain containing 2"
                     /db_xref="GeneID:23588"
                     /db_xref="HGNC:HGNC:20231"
                     /db_xref="MIM:611280"
     exon            1..469
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..1537
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /note="host cell factor homolog LCP; host cell factor-like
                     protein 1; hepatocellular carcinoma-associated antigen 33"
                     /codon_start=1
                     /product="kelch domain-containing protein 2"
                     /protein_id="NP_055130.1"
                     /db_xref="CCDS:CCDS9693.1"
                     /db_xref="GeneID:23588"
                     /db_xref="HGNC:HGNC:20231"
                     /db_xref="MIM:611280"
                     /translation="MADGNEDLRADDLPGPAFESYESMELACPAERSGHVAVSDGRHM
                     FVWGGYKSNQVRGLYDFYLPREELWIYNMETGRWKKINTEGDVPPSMSGSCAVCVDRV
                     LYLFGGHHSRGNTNKFYMLDSRSTDRVLQWERIDCQGIPPSSKDKLGVWVYKNKLIFF
                     GGYGYLPEDKVLGTFEFDETSFWNSSHPRGWNDHVHILDTETFTWSQPITTGKAPSPR
                     AAHACATVGNRGFVFGGRYRDARMNDLHYLNLDTWEWNELIPQGICPVGRSWHSLTPV
                     SSDHLFLFGGFTTDKQPLSDAWTYCISKNEWIQFNHPYTEKPRLWHTACASDEGEVIV
                     FGGCANNLLVHHRAAHSNEILIFSVQPKSLVRLSLEAVICFKEMLANSWNCLPKHLLH
                     SVNQRFGSNNTSGS"
     exon            470..549
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            550..667
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            668..783
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            784..865
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            866..949
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1030
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1089
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1199
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1272
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1360
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1413
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..4969
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1703..1708
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /note="hexamer: AATAAA"
     polyA_site      1722
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /note="major polyA site"
     regulatory      4946..4951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
                     /note="hexamer: AATAAA"
     polyA_site      4969
                     /gene="KLHDC2"
                     /gene_synonym="HCLP-1; HCLP1; LCP"
ORIGIN      
        1 agacgggctg caatagggag ccggcccgac gcggaccgct tccctgcagt gccccgagtc
       61 ccgggcccgc gccgccgccg cccggctccg ctcgcggccc ctctgtctgc aggcgtgccc
      121 cggcggcggc ggagagccgt cctcggccga ggaggctggg aaacgcgagc gcaggcggca
      181 gagaggcctc aacgccgtcc ctttcgccac cgccttttcc ttgcctcgcg ccgctgtgca
      241 tttctctcct tttcctttgt ttttttggcc cctcgcgggt gtgggcattg ttggttagca
      301 aaagtgcagc ctcaagatgg ctgatggcaa cgaggatctg cgggctgacg acttgcctgg
      361 gccagccttc gagagctatg agtccatgga gcttgcctgc cccgctgagc gcagcggcca
      421 cgtagccgtc agcgacgggc gccacatgtt cgtctggggc ggctacaaga gtaatcaagt
      481 cagaggatta tatgactttt atctgcctag agaagaacta tggatctaca acatggagac
      541 tggaagatgg aaaaaaatca acactgaagg tgatgttcct ccttctatgt caggaagctg
      601 tgctgtgtgt gtagacaggg tgctgtactt gtttggagga caccattcaa gaggcaatac
      661 caataagttc tacatgctgg attcaaggtc tacagacaga gtgttacagt gggaaagaat
      721 tgattgccaa ggaattcctc catcatcaaa ggacaaactt ggtgtctggg tatataaaaa
      781 caagttaata ttttttggag ggtatggata tttgcctgaa gataaagtat tgggaacttt
      841 tgaattcgat gaaacatctt tttggaattc aagtcatcca agaggatgga atgatcatgt
      901 acatatttta gatactgaaa catttacctg gagccagcct ataactactg gtaaagcacc
      961 ttcacctcgt gctgcccatg cctgtgcaac tgtcggaaat agaggcttcg tgtttggagg
     1021 cagatatcga gatgctagaa tgaatgatct tcactatctt aatctggata catgggagtg
     1081 gaatgaatta attccacaag gcatatgccc agttggtcga tcttggcact cactaacacc
     1141 agtttcttca gatcatcttt ttctctttgg aggatttacc actgataaac agccactaag
     1201 tgatgcctgg acttactgca tcagtaaaaa tgaatggata caatttaatc atccatatac
     1261 cgaaaaacca aggttatggc acacagcttg tgccagcgat gaaggagaag taattgtttt
     1321 tggtggatgt gccaacaact tgcttgtcca tcacagagct gcacacagta atgaaatact
     1381 aatattttca gttcaaccaa aatctcttgt acggctaagc ttagaagcag tcatttgctt
     1441 taaagaaatg ttagccaact catggaactg ccttccaaaa cacttacttc acagtgttaa
     1501 tcagaggttt ggtagtaaca acacttctgg atcttaaggc ttcataaata atgcctatga
     1561 tcaccttgca tggacagcaa tcctgtaaac atcacagagt ggcatcattt gtataattat
     1621 atgcattgtt gtagtttgca cctgttggtt ttaatgtgca tgtgaatggc ctagagaacc
     1681 tatttttgtg tctaaagttt acaataaatg tatttaacac cagtagctgt cctctattaa
     1741 agtaaagtaa tggttgggct ttttaccctg aagaatggtt gctacatttt cttttcagta
     1801 acttcaggat atgtatctgt agaaacatta tagtcttacc atcatcaaaa tgctgaagtc
     1861 attttttgaa aacattatag attttttttt ttttaatggc aagagtagtc tatagttatg
     1921 taacctagtg ttgtaaatac aatataataa aggttttttt ttttaaacat taatattcac
     1981 attatgacga agctggaaaa acaaagtgtg gagggaccaa caactgttca gtactttgac
     2041 cttaatgctt cagtgtaggg gctggagcaa gtggtcatgc tgaatacagg caggtgctgt
     2101 ccagtcaatt aaaaagtttt tttttaatta ataggtttta tagctcatga aggaatggaa
     2161 ataatgatga catcattttc cattctgtta agagacagag gaaaagtggg ttagagtctt
     2221 tagtggtaag cctaaaatgc gcttctggta ttataggtta agatactcta acgtgctata
     2281 ttggtaatta attactaaag gtggaaatta aaatggtaca gaactgaatc agattacaag
     2341 gaaaaaatta agatcaaacc tgaaggtcta caaatgaata gaaatgatta agctgagtca
     2401 tgagaattat tacttatatt ttacattttg gtctaatatt tttttctgac aagtatcaat
     2461 gaaagcagaa gaaaatccac tccaatactt agttttcaag acacaatcct aaatatttaa
     2521 accccttgta gctggcctat aatatatata tattagatgt tatatacagt agacttacca
     2581 agtcaatagt ttaagcaatc aagccacttc actgttcagt ttctttacat catgaaatga
     2641 atacttggta ttaacctccc aaatttcaag aaaatgtaga ataactacag atgtatataa
     2701 ttagcattta actgtccaca aatactttag gaaatcctat catagtgttt cctctatata
     2761 aaactgggaa aaaaacaaga attaagtcct tttggtgaat atagcaaggc aatgtttagt
     2821 tcatttggcc tgtaatatat atattagatg ttatatacag tagacttacc aagtcaatag
     2881 tttaagcaat caagccactt cactgttcag tttctttaca tcatgaaatg aatacttggt
     2941 attaacctcc caaatttcaa gaaaatgtag aataactaca gatgtatatt aattagcatt
     3001 taactgtcca caaatacttt tggaaatcct atcatagaca gtgtttcctc tatataaaac
     3061 tgggaaaaaa caagaagtaa gtccttttgg tgaatatagc aaggcaatgt ttagttcatt
     3121 tttataatgc ggaaatcctg atacatggtg ctttcatctt tgaacttcca aaaggctata
     3181 aaattggctc ttctcaaata ttttagaatt tcatttcagc tatttccttt ttacgttcag
     3241 aagattgggt gcagacactt tcactttgcc aggaatgttt cttgataaat ctgtgtccta
     3301 aaaaaagaaa attgctgaat atcttcacat cctgtatggt caatcatgtt tcttttatga
     3361 caaaaacgaa aaacatttcc aaacttttag ctgagatggt tttactgctt ctaataagac
     3421 agcattttct actaaaataa caaaaaactg ctaacatttt aaattgtact gtcagtctcc
     3481 acattccttt tctgaaggag aactttacct ttacgctgcg gaataagtct ttttctcttg
     3541 ctgttgcttc tttggaatgc atgaaactat tcaaggctgt ttttgcagct gtaaatacaa
     3601 aaaagagtaa gaataatcta ctgttaagtc actgaactgt ttaaaatcat aattcaaaaa
     3661 aacaaattta aatacctttt ccctttttct gcactccagg agtaagtttc actttatatc
     3721 tttaaaaagg aattacatgt gttactaaat agacgttaca aaacaattca caaaagccat
     3781 tctgtcaact aatggtaact tacttgtagt ttgtcatggt ggtgtaaggg gcacatattg
     3841 gaatggcaaa cagtagtaca tcttcaggat gtggctggcc tgtcaaagaa tcaaataggt
     3901 tttcctaaaa agggaaaata acagttacaa aggcaattta taccgccctt tacaaaaaat
     3961 gctaaaacac ttgaattagt atctcaacat attttttatc ttttcctgat atacatacca
     4021 tctaagctgg aacagtggta cttaaactct gcttcatagt tccaaagagt tgaagaccaa
     4081 tgtttaaata tattctttac tacttaattt ttgcctagca taataagtaa tgagaacaat
     4141 aattttcaaa atcctaccta cagttacatt taagagaaag aaggcccagg agcagtggtt
     4201 cacgcctata atcctaggac tctggaaggc tcaggctgag gcgggtggat cgcttgagcc
     4261 caggagttca agaccagcct aagcaacatg gagaaacacc atctctacaa aaaatataaa
     4321 agttagctgg gtatagtggg tgcctgtagt cccagctact cggaagactg agggggggag
     4381 gatcacctga gcccaggagg tagacgttgt ggagagccat gatcctgtca ctgcactggg
     4441 tgacaccagc ctgggtgaca gagaccctgt ctcaaaaaaa aaaaaaaaag gaaaaaaact
     4501 attggatatt acctgagttg cagaaacctg ctgtggatta aaatgatgca ataataacca
     4561 caaatagatt taagtcctat cacatggaaa tgggactaat accttggcta taccactatt
     4621 tctcgtcctg gggagattct gccctcagaa gacaatgggc aatgtctaga gatagttttc
     4681 gttgacacga tgaggggtga gaaggaatag gtaggggctg ctactgggat ctagtgggta
     4741 aaggccgagg atgtcgctaa acattttata atgaatggga cagaccccac aacaaagaat
     4801 taaccaaccc ctaatgttaa tagtgccaaa gttgagaaac cctgggctag aacagccttc
     4861 tctcttaccc attattaagg agaaatccca gagttcttat ttgctgtgct gcctgtgaga
     4921 ttgcccccat ttattttaca atatgaataa acttagattt aggacagaa
//
