LOCUS       NM_004235               2936 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens KLF transcription factor 4 (KLF4), transcript variant
            2, mRNA.
ACCESSION   NM_004235
VERSION     NM_004235.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Tan,Y., Wang,J., Liu,C., Wu,S., Zhou,M., Zhang,Y., Yin,T. and
            Yang,J.
  TITLE     KLF4 regulates trophoblast function and associates with unexplained
            recurrent spontaneous abortion
  JOURNAL   J Transl Med 22 (1), 922 (2024)
   PUBMED   39390495
  REMARK    GeneRIF: KLF4 regulates trophoblast function and associates with
            unexplained recurrent spontaneous abortion.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Zhou,J., Luo,W., Xie,Z., Xia,C. and Zhao,J.
  TITLE     KLF4-induced upregulation of SOCS1 ameliorates myocardial
            ischemia/reperfusion injury by attenuating AC16 cardiomyocyte
            damage and enhancing M2 macrophage polarization
  JOURNAL   J Biochem Mol Toxicol 38 (9), e23816 (2024)
   PUBMED   39185902
  REMARK    GeneRIF: KLF4-induced upregulation of SOCS1 ameliorates myocardial
            ischemia/reperfusion injury by attenuating AC16 cardiomyocyte
            damage and enhancing M2 macrophage polarization.
REFERENCE   3  (bases 1 to 2936)
  AUTHORS   Liang,Y., Zhao,J., Dai,T., Li,X., Chen,L., He,Z., Guo,M., Zhao,J.
            and Xu,L.
  TITLE     A review of KLF4 and inflammatory disease: Current status and
            future perspective
  JOURNAL   Pharmacol Res 207, 107345 (2024)
   PUBMED   39134187
  REMARK    GeneRIF: A review of KLF4 and inflammatory disease: Current status
            and future perspective.
            Review article
REFERENCE   4  (bases 1 to 2936)
  AUTHORS   Wang,Y., Zuo,D., Huang,Z., Qiu,Y., Wu,Z., Liu,S., Zeng,Y., Qiu,Z.,
            He,W., Li,B., Yuan,Y., Niu,Y. and Qiu,J.
  TITLE     KLF4 Suppresses the Progression of Hepatocellular Carcinoma by
            Reducing Tumor ATP Synthesis through Targeting the Mir-206/RICTOR
            Axis
  JOURNAL   Int J Mol Sci 25 (13), 7165 (2024)
   PUBMED   39000273
  REMARK    GeneRIF: KLF4 Suppresses the Progression of Hepatocellular
            Carcinoma by Reducing Tumor ATP Synthesis through Targeting the
            Mir-206/RICTOR Axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2936)
  AUTHORS   Noti,J.D., Johnson,A.K. and Dillon,J.D.
  TITLE     The leukocyte integrin gene CD11d is repressed by gut-enriched
            Kruppel-like factor 4 in myeloid cells
  JOURNAL   J Biol Chem 280 (5), 3449-3457 (2005)
   PUBMED   15561714
  REMARK    GeneRIF: a longer isoform of gut-enriched Kruppel-like factor 4
            (GKLF) we term GKLFa interacts with the CD11d promoter
REFERENCE   6  (bases 1 to 2936)
  AUTHORS   Yet,S.F., McA'Nulty,M.M., Folta,S.C., Yen,H.W., Yoshizumi,M.,
            Hsieh,C.M., Layne,M.D., Chin,M.T., Wang,H., Perrella,M.A.,
            Jain,M.K. and Lee,M.E.
  TITLE     Human EZF, a Kruppel-like zinc finger protein, is expressed in
            vascular endothelial cells and contains transcriptional activation
            and repression domains
  JOURNAL   J Biol Chem 273 (2), 1026-1031 (1998)
   PUBMED   9422764
REFERENCE   7  (bases 1 to 2936)
  AUTHORS   Ton-That,H., Kaestner,K.H., Shields,J.M., Mahatanankoon,C.S. and
            Yang,V.W.
  TITLE     Expression of the gut-enriched Kruppel-like factor gene during
            development and intestinal tumorigenesis
  JOURNAL   FEBS Lett 419 (2-3), 239-243 (1997)
   PUBMED   9428642
  REMARK    GeneRIF: This study examines the expression of KLF4 during
            embryonic and postnatal development and in intestinal
            tumorigenesis.
REFERENCE   8  (bases 1 to 2936)
  AUTHORS   Shields,J.M. and Yang,V.W.
  TITLE     Two potent nuclear localization signals in the gut-enriched
            Kruppel-like factor define a subfamily of closely related Kruppel
            proteins
  JOURNAL   J Biol Chem 272 (29), 18504-18507 (1997)
   PUBMED   9218496
  REMARK    GeneRIF: This paper describes that the nuclear localization signals
            within KLF4 define a closely related subfamily of Kruppel-like
            factors including KLF1, KLF2 and KLF4.
REFERENCE   9  (bases 1 to 2936)
  AUTHORS   Garrett-Sinha,L.A., Eberspaecher,H., Seldin,M.F. and de
            Crombrugghe,B.
  TITLE     A gene for a novel zinc-finger protein expressed in differentiated
            epithelial cells and transiently in certain mesenchymal cells
  JOURNAL   J Biol Chem 271 (49), 31384-31390 (1996)
   PUBMED   8940147
REFERENCE   10 (bases 1 to 2936)
  AUTHORS   Shields,J.M., Christy,R.J. and Yang,V.W.
  TITLE     Identification and characterization of a gene encoding a
            gut-enriched Kruppel-like factor expressed during growth arrest
  JOURNAL   J Biol Chem 271 (33), 20009-20017 (1996)
   PUBMED   8702718
  REMARK    GeneRIF: This is the first paper that describes the identification
            and characterization of KLF4 (also called GKLF) and that KLF4 is
            inhibitor of cell growth.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL360218.22, AF105036.1 and
            BU739190.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004235.5.
            
            Summary: This gene encodes a protein that belongs to the Kruppel
            family of transcription factors. The encoded zinc finger protein is
            required for normal development of the barrier function of skin.
            The encoded protein is thought to control the G1-to-S transition of
            the cell cycle following DNA damage by mediating the tumor
            suppressor gene p53. Mice lacking this gene have a normal
            appearance but lose weight rapidly, and die shortly after birth due
            to fluid evaporation resulting from compromised epidermal barrier
            function. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Sep
            2015].
            
            Transcript Variant: This variant (2) lacks an in-frame segment in
            the 3' coding region compared to variant 1. The encoded isoform (2)
            is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.896616.1, AF105036.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374672.5/ ENSP00000363804.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-295               AL360218.22        5762-6056           c
            296-2934            AF105036.1         1-2639
            2935-2936           BU739190.1         17-18               c
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q31.2"
     gene            1..2936
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /note="KLF transcription factor 4"
                     /db_xref="GeneID:9314"
                     /db_xref="HGNC:HGNC:6348"
                     /db_xref="MIM:602253"
     exon            1..602
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /inference="alignment:Splign:2.1.0"
     CDS             598..2037
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /note="isoform 2 is encoded by transcript variant 2;
                     endothelial Kruppel-like zinc finger protein;
                     krueppel-like factor 4; Kruppel like factor 4;
                     gut-enriched krueppel-like factor; epithelial zinc finger
                     protein EZF; Kruppel-like factor 4 (gut); gut Kruppel-like
                     factor"
                     /codon_start=1
                     /product="Krueppel-like factor 4 isoform 2"
                     /protein_id="NP_004226.3"
                     /db_xref="CCDS:CCDS6770.2"
                     /db_xref="GeneID:9314"
                     /db_xref="HGNC:HGNC:6348"
                     /db_xref="MIM:602253"
                     /translation="MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRW
                     REELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDL
                     LDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDP
                     GVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQP
                     QPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPK
                     IKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPAL
                     PLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKR
                     TATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKH
                     TGHRPFQCQKCDRAFSRSDHLALHMKRHF"
     exon            603..723
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /inference="alignment:Splign:2.1.0"
     exon            724..1696
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1861
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..2936
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2792..2797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /note="hexamer: AATAAA"
     polyA_site      2815
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
     regulatory      2911..2916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /note="hexamer: AATAAA"
     polyA_site      2936
                     /gene="KLF4"
                     /gene_synonym="EZF; GKLF"
                     /note="major polyA site"
ORIGIN      
        1 ggcagtttcc cgaccagaga gaacgaacgt gtctgcgggc gcgcggggag cagaggcggt
       61 ggcgggcggc ggcggcaccg ggagccgccg agtgaccctc ccccgcccct ctggcccccc
      121 accctcccac ccgcccgtgg cccgcgccca tggccgcgcg cgctccacac aactcaccgg
      181 agtccgcgcc ttgcgccgcc gaccagttcg cagctccgcg ccacggcagc cagtctcacc
      241 tggcggcacc gcccgcccac cgccccggcc acagcccctg cgcccacggc agcactcgag
      301 gcgaccgcga cagtggtggg ggacgctgct gagtggaaga gagcgcagcc cggccaccgg
      361 acctacttac tcgccttgct gattgtctat ttttgcgttt acaacttttc taagaacttt
      421 tgtatacaaa ggaacttttt aaaaaagacg cttccaagtt atatttaatc caaagaagaa
      481 ggatctcggc caatttgggg ttttgggttt tggcttcgtt tcttctcttc gttgactttg
      541 gggttcaggt gccccagctg cttcgggctg ccgaggacct tctgggcccc cacattaatg
      601 aggcagccac ctggcgagtc tgacatggct gtcagcgacg cgctgctccc atctttctcc
      661 acgttcgcgt ctggcccggc gggaagggag aagacactgc gtcaagcagg tgccccgaat
      721 aaccgctggc gggaggagct ctcccacatg aagcgacttc ccccagtgct tcccggccgc
      781 ccctatgacc tggcggcggc gaccgtggcc acagacctgg agagcggcgg agccggtgcg
      841 gcttgcggcg gtagcaacct ggcgccccta cctcggagag agaccgagga gttcaacgat
      901 ctcctggacc tggactttat tctctccaat tcgctgaccc atcctccgga gtcagtggcc
      961 gccaccgtgt cctcgtcagc gtcagcctcc tcttcgtcgt cgccgtcgag cagcggccct
     1021 gccagcgcgc cctccacctg cagcttcacc tatccgatcc gggccgggaa cgacccgggc
     1081 gtggcgccgg gcggcacggg cggaggcctc ctctatggca gggagtccgc tccccctccg
     1141 acggctccct tcaacctggc ggacatcaac gacgtgagcc cctcgggcgg cttcgtggcc
     1201 gagctcctgc ggccagaatt ggacccggtg tacattccgc cgcagcagcc gcagccgcca
     1261 ggtggcgggc tgatgggcaa gttcgtgctg aaggcgtcgc tgagcgcccc tggcagcgag
     1321 tacggcagcc cgtcggtcat cagcgtcagc aaaggcagcc ctgacggcag ccacccggtg
     1381 gtggtggcgc cctacaacgg cgggccgccg cgcacgtgcc ccaagatcaa gcaggaggcg
     1441 gtctcttcgt gcacccactt gggcgctgga ccccctctca gcaatggcca ccggccggct
     1501 gcacacgact tccccctggg gcggcagctc cccagcagga ctaccccgac cctgggtctt
     1561 gaggaagtgc tgagcagcag ggactgtcac cctgccctgc cgcttcctcc cggcttccat
     1621 ccccacccgg ggcccaatta cccatccttc ctgcccgatc agatgcagcc gcaagtcccg
     1681 ccgctccatt accaagagct catgccaccc ggttcctgca tgccagagga gcccaagcca
     1741 aagaggggaa gacgatcgtg gccccggaaa aggaccgcca cccacacttg tgattacgcg
     1801 ggctgcggca aaacctacac aaagagttcc catctcaagg cacacctgcg aacccacaca
     1861 ggtgagaaac cttaccactg tgactgggac ggctgtggat ggaaattcgc ccgctcagat
     1921 gaactgacca ggcactaccg taaacacacg gggcaccgcc cgttccagtg ccaaaaatgc
     1981 gaccgagcat tttccaggtc ggaccacctc gccttacaca tgaagaggca tttttaaatc
     2041 ccagacagtg gatatgaccc acactgccag aagagaattc agtatttttt acttttcaca
     2101 ctgtcttccc gatgagggaa ggagcccagc cagaaagcac tacaatcatg gtcaagttcc
     2161 caactgagtc atcttgtgag tggataatca ggaaaaatga ggaatccaaa agacaaaaat
     2221 caaagaacag atggggtctg tgactggatc ttctatcatt ccaattctaa atccgacttg
     2281 aatattcctg gacttacaaa atgccaaggg ggtgactgga agttgtggat atcagggtat
     2341 aaattatatc cgtgagttgg gggagggaag accagaattc ccttgaattg tgtattgatg
     2401 caatataagc ataaaagatc accttgtatt ctctttacct tctaaaagcc attattatga
     2461 tgttagaaga agaggaagaa attcaggtac agaaaacatg tttaaatagc ctaaatgatg
     2521 gtgcttggtg agtcttggtt ctaaaggtac caaacaagga agccaaagtt ttcaaactgc
     2581 tgcatacttt gacaaggaaa atctatattt gtcttccgat caacatttat gacctaagtc
     2641 aggtaatata cctggtttac ttctttagca tttttatgca gacagtctgt tatgcactgt
     2701 ggtttcagat gtgcaataat ttgtacaatg gtttattccc aagtatgcct taagcagaac
     2761 aaatgtgttt ttctatatag ttccttgcct taataaatat gtaatataaa tttaagcaaa
     2821 cgtctatttt gtatatttgt aaactacaaa gtaaaatgaa cattttgtgg agtttgtatt
     2881 ttgcatactc aaggtgagaa ttaagtttta aataaaccta taatatttta tctgaa
//
