LOCUS       NM_201521               2361 bp    mRNA    linear   PRI 23-MAR-2025
DEFINITION  Homo sapiens kinesin light chain 4 (KLC4), transcript variant 1,
            mRNA.
ACCESSION   NM_201521
VERSION     NM_201521.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 2361)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2361)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2361)
  AUTHORS   Baek,J.H., Yun,H.S., Kim,J.Y., Lee,J., Lee,Y.J., Lee,C.W.,
            Song,J.Y., Ahn,J., Park,J.K., Kim,J.S., Lee,K.H., Kim,E.H. and
            Hwang,S.G.
  TITLE     Kinesin light chain 4 as a new target for lung cancer
            chemoresistance via targeted inhibition of checkpoint kinases in
            the DNA repair network
  JOURNAL   Cell Death Dis 11 (5), 398 (2020)
   PUBMED   32457423
  REMARK    GeneRIF: Kinesin light chain 4 as a new target for lung cancer
            chemoresistance via targeted inhibition of checkpoint kinases in
            the DNA repair network.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2361)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   7  (bases 1 to 2361)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   8  (bases 1 to 2361)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   9  (bases 1 to 2361)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 2361)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA782235.1 and BC080637.1.
            
            On Nov 23, 2018 this sequence version replaced NM_201521.2.
            
            Transcript Variant: This variant (1) differs in the 5' UTR, lacks a
            portion of the 5' coding region and initiates translation at a
            downstream start codon, compared to variant 3. Variants 1, 2 and 5
            encode the same isoform (a), which has a shorter N-terminus,
            compared to isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC103727.1, SRR3476690.122936.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000347162.10/ ENSP00000340221.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DA782235.1         38-60
            24-2361             BC080637.1         1-2338
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..2361
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="kinesin light chain 4"
                     /db_xref="GeneID:89953"
                     /db_xref="HGNC:HGNC:21624"
                     /db_xref="MIM:620909"
     exon            1..55
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            56..338
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1940
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="isoform a is encoded by transcript variant 1;
                     kinesin-like protein 8"
                     /codon_start=1
                     /product="kinesin light chain 4 isoform a"
                     /protein_id="NP_958929.1"
                     /db_xref="CCDS:CCDS4883.1"
                     /db_xref="GeneID:89953"
                     /db_xref="HGNC:HGNC:21624"
                     /db_xref="MIM:620909"
                     /translation="MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVL
                     QSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEK
                     QKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDG
                     HTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLV
                     IQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLL
                     NDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHP
                     DVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASCYLKQ
                     GKYAEAETLYKEILTRAHVQEFGSVDDDHKPIWMHAEEREEMSKSRHHEGGTPYAEYG
                     GWYKACKVSSPTVNTTLRNLGALYRRQGKLEAAETLEECALRSRRQGTDPISQTKVAE
                     LLGESDGRRTSQEGPGDSVKFEGGEDASVAVEWSGDGSGTLQRSGSLGKIRDVLRRSS
                     ELLVRKLQGTEPRPSSSNMKRAASLNYLNQPSAAPLQVSRGLSASTMDLSSSS"
     exon            339..569
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            570..651
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            652..871
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            872..959
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1061
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1235
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1335
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1388
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1459
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1568
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1709
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1825
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1889
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..2361
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2340..2345
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="hexamer: AATAAA"
     polyA_site      2361
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="major polyA site"
ORIGIN      
        1 atggatttaa aggggcagta ccggcaagag cggcagccac accggcagat tgcagaccgg
       61 gcaaggtccc ccaggccagg atgtcaggcc tggtgttggg gcagcgggat gagcctgcag
      121 gccaccggct cagccaagag gagatcctgg ggagcacacg gctggtcagc caagggctag
      181 aggccctacg cagtgaacac caggccgtgc tgcaaagcct gtcccagacc attgagtgtc
      241 tgcagcaggg aggccatgag gaagggctgg tgcatgagaa ggcccggcag cttcgccgtt
      301 ctatggaaaa cattgagctc gggctgagtg aggcccaggt gatgctggct ctagccagcc
      361 acctgagcac agtggagtcg gagaaacaga agctgcgggc tcaggtgcgg cggctatgcc
      421 aggagaacca gtggctgcgg gatgagctgg ctggcaccca gcagcggcta cagcgcagtg
      481 aacaggctgt ggctcagctg gaggaggaaa agaagcacct ggagttcctg gggcagctgc
      541 ggcagtatga tgaggatgga catacctcgg aggagaaaga aggcgatgcc accaaggatt
      601 ccctggatga cctctttcct aatgaggagg aagaggaccc cagcaatggc ttgtcccgtg
      661 gtcaaggtgc tacagcagct cagcagggtg gatatgagat cccagcaagg ttgcggacgt
      721 tgcacaacct ggtgatccag tacgcagccc aaggtcgcta tgaggtggcc gtgccactct
      781 gtaagcaggc actagaggac ctggagcgca catcaggccg tggccaccct gatgtcgcca
      841 ccatgctcaa catccttgct ttggtgtatc gtgaccagaa taagtataag gaagctgccc
      901 acctgctgaa tgatgccctt agcatccggg agagcacctt gggacctgac catcctgctg
      961 tggctgccac actcaacaat ttggctgtgc tctatggcaa aaggggcaag tacaaggagg
     1021 cagagcctct gtgccagcgg gcactggaga ttcgagaaaa ggtcctgggc acgaatcatc
     1081 cagatgtggc aaaacagctg aacaacctgg ccctcttgtg ccaaaaccag ggcaagtatg
     1141 aggccgtgga acgctactac cagcgagcac tggccatcta cgaggggcag ctggggccgg
     1201 acaaccctaa tgtagcccgg accaagaaca acctggcttc ctgttacctg aaacagggca
     1261 aatatgctga ggctgagaca ctatacaaag agatcctgac ccgtgcccat gtacaggagt
     1321 ttgggtctgt ggatgatgac cacaagccca tctggatgca tgcagaggag cgggaggaaa
     1381 tgagcaaaag ccggcaccat gagggtggga caccctatgc tgagtatgga ggctggtaca
     1441 aggcctgcaa agtgagcagc cccacagtga acactactct gagaaacctg ggagctctgt
     1501 ataggcgcca gggaaagctg gaggctgctg agaccctgga ggaatgtgcc ctgcggtccc
     1561 ggagacaggg cactgaccct atcagccaga cgaaggtggc agagctgctt ggggagagtg
     1621 atggtagaag gacctcccag gagggccctg gagacagtgt gaaattcgag ggaggtgaag
     1681 atgcttctgt ggctgtggag tggtccgggg atggcagtgg gaccctgcag aggagtggct
     1741 ctcttggcaa gatccgggat gtgctccgca gaagcagtga actcttggtg aggaagctcc
     1801 aggggactga gcctcggccc tccagcagca acatgaagcg agcagcctcc ttgaactatc
     1861 tgaaccaacc tagtgcagca cccctccagg tctcccgggg cctcagtgcc agcaccatgg
     1921 acctctcttc aagcagctga cattcaaccc ggcccccagg tctgctgggt ccccccaccc
     1981 ccacagccct cacagcattc cccattgctc ctggctcttc cccaccccta ggtgggacag
     2041 tgaaggggag cagtttaacc agaagattgc tgctgccctt agggtctcag ctccctcctc
     2101 aggaatccct cttaggaagg accctcagga caccctctct gcaccctgtg gtcctctaga
     2161 gtagctagct ctgaggcccc aaggtgggta caaagcaggt atggccctca gagatgcagc
     2221 ctgctgctgg cttttcagtc agagggttgg gggctggcca gccaagctgc cttgccctgg
     2281 ccgctcttac tccctccctc tgctgtctca cttcaggtcc atgtatttca cttttcttaa
     2341 ataaaagaat caggtaacct t
//
