LOCUS       NM_001289035            2130 bp    mRNA    linear   PRI 23-MAR-2025
DEFINITION  Homo sapiens kinesin light chain 4 (KLC4), transcript variant 6,
            mRNA.
ACCESSION   NM_001289035 XM_005249474
VERSION     NM_001289035.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 2130)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2130)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2130)
  AUTHORS   Baek,J.H., Yun,H.S., Kim,J.Y., Lee,J., Lee,Y.J., Lee,C.W.,
            Song,J.Y., Ahn,J., Park,J.K., Kim,J.S., Lee,K.H., Kim,E.H. and
            Hwang,S.G.
  TITLE     Kinesin light chain 4 as a new target for lung cancer
            chemoresistance via targeted inhibition of checkpoint kinases in
            the DNA repair network
  JOURNAL   Cell Death Dis 11 (5), 398 (2020)
   PUBMED   32457423
  REMARK    GeneRIF: Kinesin light chain 4 as a new target for lung cancer
            chemoresistance via targeted inhibition of checkpoint kinases in
            the DNA repair network.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2130)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   7  (bases 1 to 2130)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   8  (bases 1 to 2130)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   9  (bases 1 to 2130)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 2130)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK297433.1 and BC080637.1.
            
            On May 31, 2019 this sequence version replaced NM_001289035.1.
            
            Transcript Variant: This variant (6) contains an alternate 5'
            terminal exon, initiates translation at a downstream start codon
            and lacks an alternate in-frame exon in the 5' coding region,
            compared to variant 3. It encodes isoform d, which is shorter than
            isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK297433.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162946, SAMN03465416
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-934               AK297433.1         1-934
            935-2130            BC080637.1         1143-2338
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..2130
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="kinesin light chain 4"
                     /db_xref="GeneID:89953"
                     /db_xref="HGNC:HGNC:21624"
                     /db_xref="MIM:620909"
     exon            1..55
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            56..338
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1709
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="isoform d is encoded by transcript variant 6;
                     kinesin-like protein 8"
                     /codon_start=1
                     /product="kinesin light chain 4 isoform d"
                     /protein_id="NP_001275964.1"
                     /db_xref="CCDS:CCDS75459.1"
                     /db_xref="GeneID:89953"
                     /db_xref="HGNC:HGNC:21624"
                     /db_xref="MIM:620909"
                     /translation="MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVL
                     QSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQEEKEGDATKDSLDDLF
                     PNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQA
                     LEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVA
                     ATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKY
                     EAVERYYQRALAIYEGQLGPDNPNVARTKNNLASCYLKQGKYAEAETLYKEILTRAHV
                     QEFGSVDDDHKPIWMHAEEREEMSKSRHHEGGTPYAEYGGWYKACKVSSPTVNTTLRN
                     LGALYRRQGKLEAAETLEECALRSRRQGTDPISQTKVAELLGESDGRRTSQEGPGDSV
                     KFEGGEDASVAVEWSGDGSGTLQRSGSLGKIRDVLRRSSELLVRKLQGTEPRPSSSNM
                     KRAASLNYLNQPSAAPLQVSRGLSASTMDLSSSS"
     exon            339..420
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            421..640
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            641..728
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            729..830
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1004
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1104
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1157
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1228
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1337
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1478
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1594
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1658
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..2130
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2109..2114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="hexamer: AATAAA"
     polyA_site      2130
                     /gene="KLC4"
                     /gene_synonym="bA387M24.3; CONDRHN; KNSL8"
                     /note="major polyA site"
ORIGIN      
        1 atggatttaa aggggcagta ccggcaagag cggcagccac accggcagat tgcagaccgg
       61 gcaaggtccc ccaggccagg atgtcaggcc tggtgttggg gcagcgggat gagcctgcag
      121 gccaccggct cagccaagag gagatcctgg ggagcacacg gctggtcagc caagggctag
      181 aggccctacg cagtgaacac caggccgtgc tgcaaagcct gtcccagacc attgagtgtc
      241 tgcagcaggg aggccatgag gaagggctgg tgcatgagaa ggcccggcag cttcgccgtt
      301 ctatggaaaa cattgagctc gggctgagtg aggcccagga ggagaaagaa ggcgatgcca
      361 ccaaggattc cctggatgac ctctttccta atgaggagga agaggacccc agcaatggct
      421 tgtcccgtgg tcaaggtgct acagcagctc agcagggtgg atatgagatc ccagcaaggt
      481 tgcggacgtt gcacaacctg gtgatccagt acgcagccca aggtcgctat gaggtggccg
      541 tgccactctg taagcaggca ctagaggacc tggagcgcac atcaggccgt ggccaccctg
      601 atgtcgccac catgctcaac atccttgctt tggtgtatcg tgaccagaat aagtataagg
      661 aagctgccca cctgctgaat gatgccctta gcatccggga gagcaccttg ggacctgacc
      721 atcctgctgt ggctgccaca ctcaacaatt tggctgtgct ctatggcaaa aggggcaagt
      781 acaaggaggc agagcctctg tgccagcggg cactggagat tcgagaaaag gtcctgggca
      841 cgaatcatcc agatgtggca aaacagctga acaacctggc cctcttgtgc caaaaccagg
      901 gcaagtatga ggccgtggaa cgctactacc agcgagcact ggccatctac gaggggcagc
      961 tggggccgga caaccctaat gtagcccgga ccaagaacaa cctggcttcc tgttacctga
     1021 aacagggcaa atatgctgag gctgagacac tatacaaaga gatcctgacc cgtgcccatg
     1081 tacaggagtt tgggtctgtg gatgatgacc acaagcccat ctggatgcat gcagaggagc
     1141 gggaggaaat gagcaaaagc cggcaccatg agggtgggac accctatgct gagtatggag
     1201 gctggtacaa ggcctgcaaa gtgagcagcc ccacagtgaa cactactctg agaaacctgg
     1261 gagctctgta taggcgccag ggaaagctgg aggctgctga gaccctggag gaatgtgccc
     1321 tgcggtcccg gagacagggc actgacccta tcagccagac gaaggtggca gagctgcttg
     1381 gggagagtga tggtagaagg acctcccagg agggccctgg agacagtgtg aaattcgagg
     1441 gaggtgaaga tgcttctgtg gctgtggagt ggtccgggga tggcagtggg accctgcaga
     1501 ggagtggctc tcttggcaag atccgggatg tgctccgcag aagcagtgaa ctcttggtga
     1561 ggaagctcca ggggactgag cctcggccct ccagcagcaa catgaagcga gcagcctcct
     1621 tgaactatct gaaccaacct agtgcagcac ccctccaggt ctcccggggc ctcagtgcca
     1681 gcaccatgga cctctcttca agcagctgac attcaacccg gcccccaggt ctgctgggtc
     1741 cccccacccc cacagccctc acagcattcc ccattgctcc tggctcttcc ccacccctag
     1801 gtgggacagt gaaggggagc agtttaacca gaagattgct gctgccctta gggtctcagc
     1861 tccctcctca ggaatccctc ttaggaagga ccctcaggac accctctctg caccctgtgg
     1921 tcctctagag tagctagctc tgaggcccca aggtgggtac aaagcaggta tggccctcag
     1981 agatgcagcc tgctgctggc ttttcagtca gagggttggg ggctggccag ccaagctgcc
     2041 ttgccctggc cgctcttact ccctccctct gctgtctcac ttcaggtcca tgtatttcac
     2101 ttttcttaaa taaaagaatc aggtaacctt
//
