LOCUS       NM_182902               3337 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens kinesin family member 9 (KIF9), transcript variant 2,
            mRNA.
ACCESSION   NM_182902
VERSION     NM_182902.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Meng,Z., Meng,Q., Gao,T., Zhou,H., Xue,J., Li,H., Wu,Y. and Lv,J.
  TITLE     Identification of bi-allelic KIF9 loss-of-function variants
            contributing to asthenospermia and male infertility in two Chinese
            families
  JOURNAL   Front Endocrinol (Lausanne) 13, 1091107 (2023)
   PUBMED   36686457
  REMARK    GeneRIF: Identification of bi-allelic KIF9 loss-of-function
            variants contributing to asthenospermia and male infertility in two
            Chinese families.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3337)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   4  (bases 1 to 3337)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
REFERENCE   5  (bases 1 to 3337)
  AUTHORS   Andrieu,G., Quaranta,M., Leprince,C. and Hatzoglou,A.
  TITLE     The GTPase Gem and its partner Kif9 are required for chromosome
            alignment, spindle length control, and mitotic progression
  JOURNAL   FASEB J 26 (12), 5025-5034 (2012)
   PUBMED   22964304
  REMARK    GeneRIF: Data show a mechanism by which GTPase Gem contributes to
            the mitotic progression by maintaining correct spindle length
            through the kinesin Kif9.
REFERENCE   6  (bases 1 to 3337)
  AUTHORS   Cornfine,S., Himmel,M., Kopp,P., El Azzouzi,K., Wiesner,C.,
            Kruger,M., Rudel,T. and Linder,S.
  TITLE     The kinesin KIF9 and reggie/flotillin proteins regulate matrix
            degradation by macrophage podosomes
  JOURNAL   Mol Biol Cell 22 (2), 202-215 (2011)
   PUBMED   21119006
  REMARK    GeneRIF: kinesin KIF9 and reggie/flotillin proteins are novel
            regulators of macrophage podosomes and their interaction is
            critical for podosomal matrix-degradation
REFERENCE   7  (bases 1 to 3337)
  AUTHORS   Piddini,E., Schmid,J.A., de Martin,R. and Dotti,C.G.
  TITLE     The Ras-like GTPase Gem is involved in cell shape remodelling and
            interacts with the novel kinesin-like protein KIF9
  JOURNAL   EMBO J 20 (15), 4076-4087 (2001)
   PUBMED   11483511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI741745.1, BC030657.2, AK314418.1, AK292547.1 and AC104447.2.
            
            On Jan 14, 2020 this sequence version replaced NM_182902.3.
            
            Transcript Variant: This variant (2) includes an alternate in-frame
            exon in the mid coding region, compared to variant 1, and encodes
            an isoform (2) that is longer than isoform 1. Both variants 2 and 4
            encode the same isoform.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292547.1, BC030657.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684063.1/ ENSP00000507186.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AI741745.1         49-249
            202-2221            BC030657.2         1-2020
            2222-2683           AK314418.1         2247-2708
            2684-3309           AK292547.1         2426-3051
            3310-3337           AC104447.2         75053-75080         c
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..3337
                     /gene="KIF9"
                     /note="kinesin family member 9"
                     /db_xref="GeneID:64147"
                     /db_xref="HGNC:HGNC:16666"
                     /db_xref="MIM:607910"
     exon            1..305
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            306..403
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     CDS             311..2683
                     /gene="KIF9"
                     /note="isoform 2 is encoded by transcript variant 2;
                     kinesin protein 9; kinesin-like protein KIF9"
                     /codon_start=1
                     /product="kinesin-like protein KIF9 isoform 2"
                     /protein_id="NP_878905.2"
                     /db_xref="CCDS:CCDS2752.1"
                     /db_xref="GeneID:64147"
                     /db_xref="HGNC:HGNC:16666"
                     /db_xref="MIM:607910"
                     /translation="MGTRKKVHAFVRVKPTDDFAHEMIRYGDDKRSIDIHLKKDIRRG
                     VVNNQQTDWSFKLDGVLHDASQDLVYETVAKDVVSQALDGYNGTIMCYGQTGAGKTYT
                     MMGATENYKHRGILPRALQQVFRMIEERPTHAITVRVSYLEIYNESLFDLLSTLPYVG
                     PSVTPMTIVENPQGVFIKGLSVHLTSQEEDAFSLLFEGETNRIIASHTMNKNSSRSHC
                     IFTIYLEAHSRTLSEEKYITSKINLVDLAGSERLGKSGSEGQVLKEATYINKSLSFLE
                     QAIIALGDQKRDHIPFRQCKLTHALKDSLGGNCNMVLVTNIYGEAAQLEETLSSLRFA
                     SRMKLVTTEPAINEKYDAERMVKNLEKELALLKQELAIHDSLTNRTFVTYDPMDEIQI
                     AEINSQVRRYLEGTLDEIDIISLRQIKEVFNQFRVVLSQQEQEVESTLRRKYTLIDRN
                     DFAAISAIQKAGLVDVDGHLVGEPEGQNFGLGVAPFSTKPGKKAKSKKTFKEPLSSLA
                     RKEGASSPVNGKDLDYVSTSKTQLVPSSKDGDVKDMLSRDRETSSIEPLPSDSPKEEL
                     RPIRPDTPPSKPVAFEEFKNEQGSEINRIFKENKSILNERRKRASETTQHINAIKREI
                     DVTKEALNFQKSLREKQGKYENKGLMIIDEEEFLLILKLKDLKKQYRSEYQDLRDLRA
                     EIQYCQHLVDQCRHRLLMEFDIWYNESFVIPEDMQMALKPGGSIRPGMVPVNRIVSLG
                     EDDQDKFSQLQQRVLPEGPDSISFYNAKVKIEQKHNYLKTMMGLQQAHRK"
     exon            404..569
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            570..676
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            677..901
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            902..985
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1078
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1226
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1291
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1369
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1438
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1543
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1599
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1690
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1824
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..2019
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2234
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2411
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..2527
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2632
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..3337
                     /gene="KIF9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2911..2916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIF9"
                     /note="hexamer: GATAAA"
     polyA_site      2940
                     /gene="KIF9"
                     /note="major polyA site"
     regulatory      3316..3321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIF9"
                     /note="hexamer: AATAAA"
     polyA_site      3337
                     /gene="KIF9"
ORIGIN      
        1 gcttcctggt tcatgtgcgg ccttcgttcc ctcgctatct cgaccttgac ttcgcatgtg
       61 catccgccga agtctttcta gatcaatcgg ccccatctct cgtcttcccg attcggccat
      121 gacctcaccc gtatcctcga cgtaggacct cggacttcat ttccgccacc ccgacacttc
      181 cggaatcccc ggaagcgacc cgttgccatg gcgactatgt acacagccag gcagccccgc
      241 gtggggagtg caggtggttt cggttgcggc agtcgcgtcc cgggagcgtc gctgcctggt
      301 gaacgctaga atgggtacta ggaaaaaagt tcatgcattt gtccgtgtca aacccaccga
      361 tgactttgct catgaaatga tcagatacgg agatgacaaa agaagcattg atattcactt
      421 aaaaaaagac attcggagag gagttgtcaa taaccaacag acagactggt cgtttaagtt
      481 ggatggagtt cttcacgatg cctcccagga cttggtttat gagacagttg caaaggatgt
      541 ggtttctcag gccctcgatg gctataatgg caccatcatg tgttatgggc agacgggagc
      601 tggcaagaca tacaccatga tgggggcaac tgagaattac aagcaccggg ggatcctccc
      661 tcgtgccctg cagcaggttt ttaggatgat cgaagaacgc cccacacatg ccatcactgt
      721 gcgtgtttcc tacttggaaa tctataatga gagcctgttt gatctcctgt ccactctgcc
      781 ctatgttgga ccctcagtca caccaatgac catcgtggaa aaccctcaag gagtcttcat
      841 taagggcttg tcagttcacc tcacaagtca ggaggaggat gcattcagcc tcctttttga
      901 gggtgagacc aacaggatta tagcctccca cactatgaac aaaaactctt ccagatcaca
      961 ctgcattttc accatctact tagaggccca ttcccggacc ttatcagagg aaaagtacat
     1021 cacttccaaa attaacttgg tggatctggc aggctcagag aggctgggga agtctgggtc
     1081 tgagggccaa gtcctgaagg aagccaccta catcaacaaa tcgctctcat tcctggagca
     1141 ggccatcatt gcccttgggg accagaagcg ggaccacatc ccctttcggc agtgcaagct
     1201 cacccacgct ctgaaggact cgttaggggg aaactgcaat atggtcctcg tgacaaacat
     1261 ctatggagaa gctgcccagt tagaagaaac gctatcttca ctgagatttg ccagcaggat
     1321 gaagctagtc accactgagc ctgccatcaa tgaaaagtat gatgctgaga gaatggtcaa
     1381 gaacctggag aaggaactag cactactcaa gcaggagctg gctatccatg acagcctgac
     1441 caaccgcacc tttgtgacct atgaccccat ggatgaaatc cagattgctg agatcaactc
     1501 ccaggtgcgg aggtacctgg aggggacact ggacgagatc gacataatca gccttagaca
     1561 gatcaaggag gtgttcaacc agttccgggt ggttctgagc caacaggaac aggaagtgga
     1621 gtccactttg cgcaggaagt acaccctcat tgacaggaat gactttgcag ccatttctgc
     1681 tatccagaag gcggggcttg tggatgttga tggccaccta gtgggtgagc ctgaaggaca
     1741 aaactttgga ctcggagtcg cccctttctc taccaaacct gggaagaaag ccaagtccaa
     1801 gaagacattc aaagagccac tcagctcctt ggcaagaaag gaaggtgcca gcagccctgt
     1861 gaatgggaag gacttggatt acgtttccac ctccaagacc cagctggtcc catcctccaa
     1921 agatggggat gtcaaagaca tgctttcgcg ggaccgggaa acttccagca ttgagcccct
     1981 tccctcagac tccccgaagg aggaattacg cccaattagg cccgacaccc caccctccaa
     2041 accagtggcc tttgaggagt ttaagaatga gcaaggtagt gagatcaacc gaattttcaa
     2101 agaaaacaaa tccatcttga atgaacggag gaaaagggcc agcgagacca cacagcacat
     2161 caatgccatc aagcgggaga ttgatgtgac caaggaggcc ctgaatttcc agaagtcact
     2221 acgggagaag caaggcaagt acgaaaacaa ggggctgatg atcatcgatg aggaagaatt
     2281 cctgctgatc ctcaagctca aagacctcaa gaagcagtac cgcagcgagt accaggacct
     2341 gcgtgacctc agggctgaga tccagtattg ccagcaccta gtggatcagt gtcgccaccg
     2401 cctgctcatg gaatttgaca tctggtacaa tgagtccttt gtcatccctg aggacatgca
     2461 gatggcactg aagccaggcg gcagcatccg gccaggcatg gtccctgtga acaggattgt
     2521 gtctctggga gaagatgacc aggacaaatt cagccagctg cagcagaggg tgcttcctga
     2581 gggccctgat tccatctcct tctacaatgc caaagtcaag atagagcaga agcataatta
     2641 cttgaaaacc atgatgggcc tccagcaggc acatagaaaa tagaacctca tcgccagtac
     2701 cttaaaggac aagaccagca actcccacct actgtagtgg agctgctcaa ccacctgccc
     2761 agagctgcag ccccctctac tccaatgctg ggacccagca cagagaacac atttggcctg
     2821 catgttggga ggagcatcct ccaaggacaa ccttgctcat ctccacagag cactttgggt
     2881 tttaattcac tgtcttatat gcagggacag gataaaataa ctttctagtt tggactttca
     2941 gtgctcatct gagaactttg tcttatttcc tgtctgaaag gacatattct ctatgtatgt
     3001 gtgaggtcag aactgagtct caagtaggaa ggcagaaaag tagctcagcc aggctcctcc
     3061 tggcaggcct ttgtcacact ggtcagcccc tttcctggat agacatccta gggtgggggt
     3121 ggtgagctcg gtctgaggag ctctgcttct cacagctccc tcagacccta agttctctca
     3181 agacatcttt tctgtgtttc tgagccagtg aagaagacag gaggaggccc tccttcattt
     3241 ggcaaccagg ggaggataag gtctagccac agagaccaga aagggatgag gcagagctgg
     3301 ctccatgtac cagggaataa atgagcagct tctggga
//
