LOCUS       NM_030615               3048 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens kinesin family member 25 (KIF25), transcript variant
            1, mRNA.
ACCESSION   NM_030615 XM_011535802
VERSION     NM_030615.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Decarreau,J., Wagenbach,M., Lynch,E., Halpern,A.R., Vaughan,J.C.,
            Kollman,J. and Wordeman,L.
  TITLE     The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome
            separation to establish proper spindle orientation
  JOURNAL   Nat Cell Biol 19 (4), 384-390 (2017)
   PUBMED   28263957
  REMARK    GeneRIF: The activity of Kif25 during interphase is required to
            maintain a centred nucleus to ensure the spindle is stably oriented
            at the onset of mitosis.
            Erratum:[Nat Cell Biol. 2017 May 31;19(6):740. doi:
            10.1038/ncb3546.. PMID: 28561052]
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Luykx,J.J., Bakker,S.C., Lentjes,E., Neeleman,M., Strengman,E.,
            Mentink,L., DeYoung,J., de Jong,S., Sul,J.H., Eskin,E., van
            Eijk,K., van Setten,J., Buizer-Voskamp,J.E., Cantor,R.M., Lu,A.,
            van Amerongen,M., van Dongen,E.P., Keijzers,P., Kappen,T.,
            Borgdorff,P., Bruins,P., Derks,E.M., Kahn,R.S. and Ophoff,R.A.
  TITLE     Genome-wide association study of monoamine metabolite levels in
            human cerebrospinal fluid
  JOURNAL   Mol Psychiatry 19 (2), 228-234 (2014)
   PUBMED   23319000
REFERENCE   3  (bases 1 to 3048)
  AUTHORS   McKnight,N.C., Jefferies,H.B., Alemu,E.A., Saunders,R.E.,
            Howell,M., Johansen,T. and Tooze,S.A.
  TITLE     Genome-wide siRNA screen reveals amino acid starvation-induced
            autophagy requires SCOC and WAC
  JOURNAL   EMBO J 31 (8), 1931-1946 (2012)
   PUBMED   22354037
REFERENCE   4  (bases 1 to 3048)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   5  (bases 1 to 3048)
  AUTHORS   Miki,H., Setou,M., Kaneshiro,K. and Hirokawa,N.
  TITLE     All kinesin superfamily protein, KIF, genes in mouse and human
  JOURNAL   Proc Natl Acad Sci U S A 98 (13), 7004-7011 (2001)
   PUBMED   11416179
  REMARK    Review article
REFERENCE   6  (bases 1 to 3048)
  AUTHORS   Okamoto,S., Matsushima,M. and Nakamura,Y.
  TITLE     Identification, genomic organization, and alternative splicing of
            KNSL3, a novel human gene encoding a kinesin-like protein
  JOURNAL   Cytogenet Cell Genet 83 (1-2), 25-29 (1998)
   PUBMED   9925916
REFERENCE   7  (bases 1 to 3048)
  AUTHORS   Erdel,M. and Weiskirchen,R.
  TITLE     Assignment1 of CSRP1 encoding the LIM domain protein CRP1, to human
            chromosome 1q32 by fluorescence in situ hybridization
  JOURNAL   Cytogenet Cell Genet 83 (1-2), 10-11 (1998)
   PUBMED   9925910
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL589733.20.
            
            On or before Oct 19, 2020 this sequence version replaced
            XM_011535802.3, NM_030615.3.
            
            Summary: The protein encoded by this gene is a member of the
            kinesin-like protein family. Protein family members are
            microtubule-dependent molecular motors that transport organelles
            within cells and move chromosomes during cell division. However,
            the particular function of this gene product has not yet been
            determined. Two alternatively spliced transcript variants which
            encode products have been described. Other splice variants have
            been found that lack exon 2 and the initiation codon for
            translation. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.107169.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000643607.3/ ENSP00000496229.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1200              AL589733.20        21362-22561
            1201-1429           AL589733.20        22783-23011
            1430-1546           AL589733.20        26234-26350
            1547-1636           AL589733.20        27305-27394
            1637-1704           AL589733.20        41664-41731
            1705-1890           AL589733.20        53183-53368
            1891-1965           AL589733.20        54464-54538
            1966-2115           AL589733.20        57573-57722
            2116-2292           AL589733.20        62244-62420
            2293-2444           AL589733.20        63756-63907
            2445-2627           AL589733.20        65660-65842
            2628-2783           AL589733.20        66252-66407
            2784-3048           AL589733.20        68518-68782
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..3048
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /note="kinesin family member 25"
                     /db_xref="GeneID:3834"
                     /db_xref="HGNC:HGNC:6390"
                     /db_xref="MIM:603815"
     exon            1..1200
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1429
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1546
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1636
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1704
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1890
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             1799..2953
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinesin-like 3; kinesin-like protein KIF25; kinesin-like
                     protein 3"
                     /codon_start=1
                     /product="kinesin-like protein KIF25 isoform 1"
                     /protein_id="NP_085118.2"
                     /db_xref="CCDS:CCDS5305.1"
                     /db_xref="GeneID:3834"
                     /db_xref="HGNC:HGNC:6390"
                     /db_xref="MIM:603815"
                     /translation="MTWTSGQLQREKQARPGSGAVLAFPDDKDLRVYGPAESQSAVFG
                     DVCPLLTSLLDGYNVCVMAYGQTGSGKSYTMLGRHSDDGPVLPLDPQSDLGIIPRVAE
                     ELFRLILENTSRSPKVEVSIVEVYNNDIFDLLAKDSIAAVSGVKREVVTAKDGRTEVA
                     LLASEAVGSASKLMELVHGGLQLRAKHPTLVHADSSRSHLIITVTLTTASCSDSTADQ
                     ACSATLPREQTEAGRAGRSRRASQGALAPQLVPGNPAGHAEQVQARLQLVDSAGSECV
                     GVSGVTGLALREMACISRSLAALAGVLGALLEHRGHAPYRNSRLTHLLQDCLGGDAKL
                     LVILCISPSQRHLAQTLQGLGFGIRARQVQRGPARKKPPSSQTEGKRRPD"
     exon            1891..1965
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2115
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2292
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2293..2444
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2627
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..2783
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2784..3048
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3023..3028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /note="hexamer: AATAAA"
     polyA_site      3048
                     /gene="KIF25"
                     /gene_synonym="KNSL3"
                     /note="major polyA site"
ORIGIN      
        1 ctctcgtgct ttcttggtcg aaggtatgag acctgagatc ccatgaaatt taatgtaaag
       61 tctcagggtt tgattttgca acacgaggat gccaaggtgg atgattgagt gggtgaccct
      121 cgcttctgtg aggtttgatt ttgatggttg cactttggcc agtttctccg aattccacct
      181 gcccgaggcc cacgaagcca gtgttcctgc gaagcttctc ttccctggcc atgccgagag
      241 gttctccagc tgttagatgt gtcaaagaat aattctcatt ttaagcatct acttatgtag
      301 ttcagtgctc caaacattga tataagagaa catttattga gtgcttcctg tgtaccaagt
      361 acttttgaaa ccatttggtg tattttctta tttaatcttc acaatagcac cacctctctg
      421 ggttgctata aagacaaagg gagatatcag tgaagcacct ggcacttcag aaagtctcac
      481 tacgctgcag cttttttttt ttttaatctg gagcatgaaa aagataatat gaaccttcct
      541 aagatggagt caagtcccac aaggaagaat tggttgtgga agtttctgag tgttccacct
      601 caagcctggt ctcctggtct agctgaggag cgtgcagtgc gcggcccatg gagaatatgc
      661 aggcagaggc tgatcccagc ttctgtgggt tcatagagac cagacggagg ctgtaaatca
      721 ttggcctatg acacccactt ggttttcaaa atgtagaagg agctgctcag catgacaggg
      781 tggataaact tgctgtggat ccaagacaag atttcagaat ggatttggca agttctgagc
      841 acatacggag aagatgtgtt gctcactgga aaccagtgga gtttatgaga ataaaaagct
      901 tctaatttcc ttccagtcat taaatcaagg aaatatgaca gatactgaat ttccaaaatc
      961 ccctctaaaa tgcctaaaat gtcgagccca ggatgccatt caaagggatt ttccttggta
     1021 cttattacca agggataccc ctttgccaag tgaacacatt tgtgaagtat gaatccagaa
     1081 gtttaaaata actgttcatg gccgggcacg gtggctcatg cctataatcc cagaactttg
     1141 ggaggccgag gcgggcgcat tacctgaggt caggagttcg agaccagctt ggccaacatg
     1201 gggtctttcg aagtggagac aggtgctgca gatctcacgg ccgcgtctca ggagctggtg
     1261 cccggtgcac gttcagtggg ggatgctccg cagtgcaggc tcgcgcccgg gttgacacgc
     1321 agacctcttg atcattgcag gagctgaaac gaaatatcag agttcactgt cggattcgtc
     1381 ctttgttgcc ttttgatagt gaatccgacg atccagtttt gcagagcaga ttcatgatgg
     1441 cttgcagatg cttgatttcc tgaaggggag aacctgatga gcttgtcttc tagatcagtg
     1501 gtccccaacc tttttggcat cagggaccag cttcctggaa gaccagctcc atctctagag
     1561 aagcggccca tgctgttgat gacctgagtc tacaagtttc ctcacaaatt gtattcagag
     1621 agcagcttcc tgcttggaaa caattctggt gaaatgtgat cgtcctggcc accctctgat
     1681 tgataagaca tatcattttg aaagatatac tggccttccc agctgacatg gacctcaggt
     1741 cagcttcagc gtgagaagca ggccaggcct gggtctggag ccgtcctggc cttcccagat
     1801 gacatggacc tcaggtcagc ttcagcgtga gaagcaggcc aggcctgggt ctggagccgt
     1861 cctggccttc ccagatgaca aggacctcag ggtttatggt ccagcagagt ctcagagcgc
     1921 ggtctttgga gatgtgtgcc ccctactcac ttctctcttg gatgggtaca atgtttgtgt
     1981 tatggcgtat ggacagacgg gcagcggaaa gagctatacc atgctgggac gccattcgga
     2041 cgacggccct gttctgccgc ttgacccaca gagtgactta ggaattatcc ctagagtggc
     2101 tgaggagctc ttcaggctca ttttggaaaa tacctcaaga agcccaaagg ttgaagtctc
     2161 catagtggaa gtttacaata atgacatttt tgaccttctg gccaaagaca gcattgcagc
     2221 agtgtcgggg gtcaagcgtg aggtggtgac agccaaggat ggacggacag aggttgcgct
     2281 gctggcctct gaggctgtcg gcagcgcctc gaaactgatg gagctcgttc atggaggtct
     2341 gcagctcagg gcgaagcacc ccaccctggt gcacgcggat tcctccaggt ctcacctgat
     2401 aattacggtg actctaacca cagcctcctg ctctgacagc actgcagacc aagcctgcag
     2461 tgccaccctc cccagggagc aaacagaggc aggaagggca ggaaggagcc gcagagcttc
     2521 tcaaggggcc ttggctccac agctggttcc tgggaacccc gcagggcatg cggagcaggt
     2581 gcaggctcga ctacagctcg tggactcggc cggcagcgag tgcgttggtg tgtctggagt
     2641 gaccgggttg gccctgaggg agatggcgtg catcagccgc agccttgcgg ccctggcagg
     2701 cgtcctgggg gctttgttgg agcaccgtgg ccatgccccg taccggaaca gcaggctcac
     2761 ccacctcctt caggactgcc tcggaggcga tgcgaagtta ctggtgattc tctgcatttc
     2821 tcccagccag aggcacctgg cacagacgtt gcagggcctg ggtttcggga tccgagctcg
     2881 gcaagtccag cgaggccctg cccgaaagaa gccgcccagc tcccaaacgg aggggaagag
     2941 gaggccggat tgaatgcatt aacaagtttt tctcctaaaa ctgtgtttct tgtccttgct
     3001 ttataatgca tatgtgctta gaaataaaca ggtttcacgt ggactcaa
//
