LOCUS       NM_152440               4159 bp    mRNA    linear   PRI 23-FEB-2025
DEFINITION  Homo sapiens KICSTOR subunit 2 (KICS2), transcript variant 2, mRNA.
ACCESSION   NM_152440
VERSION     NM_152440.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Wolfson,R.L., Chantranupong,L., Wyant,G.A., Gu,X., Orozco,J.M.,
            Shen,K., Condon,K.J., Petri,S., Kedir,J., Scaria,S.M.,
            Abu-Remaileh,M., Frankel,W.N. and Sabatini,D.M.
  TITLE     KICSTOR recruits GATOR1 to the lysosome and is necessary for
            nutrients to regulate mTORC1
  JOURNAL   Nature 543 (7645), 438-442 (2017)
   PUBMED   28199306
  REMARK    GeneRIF: identification of a protein complex (KICSTOR) that is
            composed of four proteins, KPTN, ITFG2, C12orf66 and SZT2, and that
            is required for amino acid or glucose deprivation to inhibit mTORC1
            in cultured human cells
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Li,L., Fridley,B.L., Kalari,K., Niu,N., Jenkins,G., Batzler,A.,
            Abo,R.P., Schaid,D. and Wang,L.
  TITLE     Discovery of genetic biomarkers contributing to variation in drug
            response of cytidine analogues using human lymphoblastoid cell
            lines
  JOURNAL   BMC Genomics 15, 93 (2014)
   PUBMED   24483146
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC012158.32 and KF459550.1.
            
            On Jun 2, 2019 this sequence version replaced NM_152440.4.
            
            Transcript Variant: This variant (2) contains a 3' terminal exon
            that extends past a splice site that is used in variant 1. This
            results in a novel 3' coding region and 3' UTR compared to variant
            1. The encoded isoform (2) shorter, and has a distinct C-terminus,
            compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.7393.1, AK057111.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398055.8/ ENSP00000381132.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-294               AC012158.32        103569-103862
            295-580             KF459550.1         8-293
            581-4159            AC012158.32        131207-134785
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14.2"
     gene            1..4159
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="KICSTOR subunit 2"
                     /db_xref="GeneID:144577"
                     /db_xref="HGNC:HGNC:26517"
                     /db_xref="MIM:617420"
     exon            1..294
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1397
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="isoform 2 is encoded by transcript variant 2;
                     UPF0536 protein C12orf66; KICSTOR complex protein
                     C12orf66"
                     /codon_start=1
                     /product="KICSTOR subunit 2 isoform 2"
                     /protein_id="NP_689653.4"
                     /db_xref="CCDS:CCDS41803.1"
                     /db_xref="GeneID:144577"
                     /db_xref="HGNC:HGNC:26517"
                     /db_xref="MIM:617420"
                     /translation="MGESIPLAAPVPVEQAVLETFFSHLGIFSYDKAKDNVEKEREAN
                     KSAGGSWLSLLAALAHLAAAEKVYHSLTYLGQKLGGQSFFSRKDSIRTIYTSLHNELK
                     KVVTGRGALGGTAPHVEELLSHLSEQLCFFVQARMEIADFYEKMYTLSTQKFINAEEL
                     VGLLDAIMKKYSSRFHHPILSPLESSFQLEVDVLCHLLKAQAQVSEWKFLPSLVNLHS
                     AHTKLQTWGQIFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLKNMLLAKFSFYFHEA
                     LSRQTTASEMKTLTAKANPDFFGKISSFIRKYDAANVSLIFDNRGSESFQGHGYHHPH
                     SYREAPKGVDQYPAVVSLPSDRPVMHWPNVIMIMTDRTSDLNSLEKVVHFYDDKVQST
                     YFLTRPEPHFTIVIIFESKKSERDSHFISFLNEVSLALKNPKVFASLKPGAKG"
     exon            295..580
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /inference="alignment:Splign:2.1.0"
     exon            581..4159
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1802..1807
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="hexamer: ATTAAA"
     polyA_site      1825
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="major polyA site"
     regulatory      2576..2581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="hexamer: AATAAA"
     polyA_site      2600
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
     regulatory      4015..4020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="hexamer: AATAAA"
     polyA_site      4043
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
     regulatory      4138..4143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
                     /note="hexamer: ATTAAA"
     polyA_site      4159
                     /gene="KICS2"
                     /gene_synonym="C12orf66; MRT83"
ORIGIN      
        1 agaacccgga agtgcgaggc cgaggagagc cacgtgggtc gagctggggc gcagctcgca
       61 tgggggagtc tatcccgctg gccgccccgg tcccggtgga acaggcggtg ctggagacgt
      121 tcttctctca cctgggtatc ttctcttacg acaaggctaa ggacaatgtg gagaaggaac
      181 gagaggccaa caagagcgcg gggggcagct ggctgtcgct gctggcggcc ttggcgcacc
      241 tggccgcggc cgagaaggtc tatcacagcc tcacctacct ggggcagaaa ctagggggcc
      301 agtctttctt cagcaggaag gattccatcc gcaccatcta tacttcattg cataatgagc
      361 tgaagaaggt ggtgactggc cgtggtgccc tgggtgggac tgctcctcac gtggaagaac
      421 tcctttccca cctgtcagag cagctctgct tctttgttca ggctcggatg gagatagcag
      481 acttctatga gaagatgtac accctcagca cacagaagtt catcaatgct gaagagctcg
      541 ttggcctttt ggatgccatc atgaaaaaat acagctccag atttcaccac ccaatcctca
      601 gtcctttgga aagcagtttc cagctggaag ttgacgtcct gtgtcatctc ctgaaagccc
      661 aggcccaggt ctcagagtgg aagttcctcc catctctggt taatttacac agtgcgcaca
      721 ccaaactgca gacgtggggc cagatctttg agaaacagcg ggagaccaag aaacatctgt
      781 ttggagggca gtctcagaag gccgtgcagc ctccacacct tttcctttgg ctgatgaaac
      841 tcaaaaacat gcttcttgcc aagtttagct tttactttca tgaggctctg agccgacaaa
      901 cgactgcttc agaaatgaaa acgttgacag caaaagctaa cccagacttt tttggaaaga
      961 tttccagctt catcagaaaa tatgatgctg ccaatgtgtc cttaattttt gacaacagag
     1021 gttctgagag ttttcagggt catggttatc accaccccca ttcctacaga gaggccccca
     1081 agggtgtgga ccagtatcca gctgtagtgt ctctgcccag cgacaggcca gtcatgcact
     1141 ggcccaatgt catcatgatc atgacggacc gcacatctga tctgaacagc ttggagaaag
     1201 tggtccactt ctatgatgac aaagttcaga gtacctactt cctaacccgc ccggaacctc
     1261 actttaccat tgtcatcatt tttgagtcaa agaaatctga gagagactcc cactttattt
     1321 ccttcctcaa tgaggtctca cttgccctta agaaccccaa agtgtttgca agtctgaaac
     1381 ctggagccaa aggttagcag gtgatttgcg tggaggggtt tcaagactag aaactgttct
     1441 taattgccct aatgatctac ggtggtctgt gaatagagtt tacatccatt catgcctctt
     1501 tcagaactaa attaaagaca aatcctccct gtgttgcaca ctttataagc aattaaggcc
     1561 aattgaatta agtttccaaa gagtaatcta ggaagtggtc atgggctacc atgtgtctcc
     1621 atggtacagc aaagtagttt cagtattttc ccctattttg ctatatttct tgtagattga
     1681 atatttattg ggcttttttt tttcccatat gtttttctgt tgttctaaac attgtgttct
     1741 actgcaattc caaacagcct ctctgtttcc ctccttcttg gaagagtaat agggatgaga
     1801 tattaaatgg cctttttaga attaagaact atatgttttc tgtctcataa attagcaaat
     1861 gaatatggag ttagtagatt tatgattctg atctacaaat taacattttt gatgccaaaa
     1921 ttagggtctt ggtaagaaat ttctatcagt tattgaaact gaaagtgagt cagattggta
     1981 gcatgacgta aggttttaac agttcagaag atcctcatgg cagctttaat agccatgggt
     2041 tcttttgggg taatgtacac acctaactgc atttgttttg acttctttaa tgtttgaatg
     2101 tttcactctg ttgtaaagtc agcataagcc atcatgtggg ctttcttcat tcttcagcat
     2161 attatccgct tcgctttctg tttatcactt ttctgctgaa atgtacatgt ttggctctac
     2221 tccttggtta tttttaggct taaaagcatg cagtcggttt ttcagccact tttagatact
     2281 catgcaacat agcctacttt tataaaatca aaatgaaggt cttcatacgc tgtacttgct
     2341 aaaaatcaac tgtgcgcttg aaagacctct tcatagttga gtaatcacct cagtacttaa
     2401 gcacttctga acttgggttt catgcctgcc ccccacaaat tatgtttaga agcagcactg
     2461 catcttagca catgctgcct tatttctttc cttcggcagg tgttcatggg aagtaggtgg
     2521 atcagtgaag gcataatggg ctgtggagac cttcacttct agattattga ttcaaaataa
     2581 aaagaagcca caaagagtaa ttgagttgtt actggtttga tggttccttt gaagcttgtg
     2641 tgaaatgtaa tggtcatatt cgtctttttt cccatgattg tgtggagcca ctgttttact
     2701 gggtgtccac agcaggtatg ccagcaacat cagcagagac cagagagccc ctcagagtcc
     2761 atgtaggaag cctgcccacc tagggtggac tgcctggcag aagaaagaag aaccggccag
     2821 gcacggtggc acacgcctgt aatcccagta ctttgggagg ctgagacgcg tggatcactt
     2881 gaggtcagga gttcgagatc agcgtggcca acatggtgaa accccatctc tactaaaaat
     2941 acaaaagttg gccggttgta gtgacgggtg actgtaatcc cagttactct ggaggctgag
     3001 gcaggagaat tgcttgaacc tgggaggtgg aggatgcagt gagctgagac tgtgcagctg
     3061 cactccagcc tgggtgaaag agcgagacct gtctcaaaaa aaaaaaaaga agaagaagaa
     3121 ccaagaatct actggcctat tttttatttt ctgggaagaa catgtgtttt tccttttttt
     3181 tttttttttt ttttgagaca gggtcttgtc tgttttccag gctggagtac agtggcacaa
     3241 tcacaactca ctgcagcctc aacctcctgg gctcaagtga tcctcccaca tcagcatccc
     3301 gagtaggtgg gattacaggc atgagccatc acacctgggt ctcactatgt tgctcgggct
     3361 ggactcaaac tcctggactc acatgagcct cccgcctcgg cctcccaaag cactgggttt
     3421 ataagcatgt gccactgcac ctggcctcct ttctttttct ttaaaaatgg tcataagctt
     3481 tgtaaaaagc tagtttgctt taaatagaaa gtagtgattt acctctgcat tttattcact
     3541 caggttctat aaaaaaagtc tcagcagatt ttacaacatt gaccttacct aagaaattga
     3601 aagcaattgg agctaaaaag tcaaatcact tttcctctct attgtgttct gccaagaaac
     3661 tgaaaaatat tccttggtat gcatgtattt ttttcctctt agattaaacc accattagcc
     3721 ttaatgacac agtcccccaa atacctagaa gaatatatac ttcagtggaa ccccgagaga
     3781 ggtgtaagta aagcatatat atgttattta aataaccagt cctcacctag aacatatttg
     3841 aattgtatat ctatttttaa acataaacac aaaaagaatt tcaaagaaca gaaggcagat
     3901 gtgaatatta agaaaatcta agacaatatg tattactgtg cagctgtcat agactgtact
     3961 ggaagagttt ttgaagattg aatattgata cttaaattat ttctgaagct cttcaataaa
     4021 gttttctaat tataaaaact ccacataatt tggttcaagc caagaaacaa agtcatttta
     4081 ctctaagatt tattctctaa atatagtaat gagatctgta aagtctgtgt tttgctaatt
     4141 aaaagagtaa atctgcgta
//
