LOCUS       NM_001164665           12498 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.
ACCESSION   NM_001164665
VERSION     NM_001164665.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Ye,F., Xie,Y., Lin,M., Liu,Y., Fang,Y., Chen,K., Zhang,Y. and
            Ding,Y.
  TITLE     KIAA1549 promotes the development and chemoresistance of colorectal
            cancer by upregulating ERCC2
  JOURNAL   Mol Cell Biochem 479 (3), 629-642 (2024)
   PUBMED   37140813
  REMARK    GeneRIF: KIAA1549 promotes the development and chemoresistance of
            colorectal cancer by upregulating ERCC2.
REFERENCE   2  (bases 1 to 12498)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12498)
  AUTHORS   Uribe,M.L., Martin-Nieto,J., Quereda,C., Rubio-Fernandez,M.,
            Cruces,J., Janssen,G.M.C., de Ru,A.H., van Veelen,P.A. and
            Hensbergen,P.J.
  TITLE     Retinal Proteomics of a Mouse Model of Dystroglycanopathies Reveals
            Molecular Alterations in Photoreceptors
  JOURNAL   J Proteome Res 20 (6), 3268-3277 (2021)
   PUBMED   34027671
REFERENCE   4  (bases 1 to 12498)
  AUTHORS   Sun,M., Wang,L., Lu,D., Zhao,Z., Teng,L., Wang,W. and Piao,Y.
  TITLE     Concomitant KIAA1549-BRAF fusion and IDH mutation in Pediatric
            spinal cord astrocytoma: a case report and literature review
  JOURNAL   Brain Tumor Pathol 38 (2), 132-137 (2021)
   PUBMED   33641074
  REMARK    GeneRIF: Concomitant KIAA1549-BRAF fusion and IDH mutation in
            Pediatric spinal cord astrocytoma: a case report and literature
            review.
            Review article
REFERENCE   5  (bases 1 to 12498)
  AUTHORS   Badiali,M., Gleize,V., Paris,S., Moi,L., Elhouadani,S., Arcella,A.,
            Morace,R., Antonelli,M., Buttarelli,F.R., Figarella-Branger,D.,
            Kim,Y.H., Ohgaki,H., Mokhtari,K., Sanson,M. and Giangaspero,F.
  TITLE     KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse
            gliomas of adults
  JOURNAL   Brain Pathol 22 (6), 841-847 (2012)
   PUBMED   22591444
  REMARK    GeneRIF: Our results suggest that in a small fraction of diffuse
            gliomas, KIAA1549-BRAF fusion gene and BRAF(v600E) mutation may be
            responsible for deregulation of the Ras-RAF-ERK signaling pathway
REFERENCE   6  (bases 1 to 12498)
  AUTHORS   Lin,A., Rodriguez,F.J., Karajannis,M.A., Williams,S.C., Legault,G.,
            Zagzag,D., Burger,P.C., Allen,J.C., Eberhart,C.G. and Bar,E.E.
  TITLE     BRAF alterations in primary glial and glioneuronal neoplasms of the
            central nervous system with identification of 2 novel KIAA1549:BRAF
            fusion variants
  JOURNAL   J Neuropathol Exp Neurol 71 (1), 66-72 (2012)
   PUBMED   22157620
  REMARK    GeneRIF: KIAA1549:BRAF fusions predominate in pilocytic
            astrocytomas but are also present in some low-grade unclassifiable
            gliomas and glioneuronal tumors.
REFERENCE   7  (bases 1 to 12498)
  AUTHORS   Hasselblatt,M., Riesmeier,B., Lechtape,B., Brentrup,A., Stummer,W.,
            Albert,F.K., Sepehrnia,A., Ebel,H., Gerss,J. and Paulus,W.
  TITLE     BRAF-KIAA1549 fusion transcripts are less frequent in pilocytic
            astrocytomas diagnosed in adults
  JOURNAL   Neuropathol Appl Neurobiol 37 (7), 803-806 (2011)
   PUBMED   21696415
  REMARK    GeneRIF: The frequency of BRAF-KIAA1549 fusion transcripts is
            significantly lower in adult patients with pilocytic astrocytoma.
REFERENCE   8  (bases 1 to 12498)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   9  (bases 1 to 12498)
  AUTHORS   Jones,D.T., Kocialkowski,S., Liu,L., Pearson,D.M., Backlund,L.M.,
            Ichimura,K. and Collins,V.P.
  TITLE     Tandem duplication producing a novel oncogenic BRAF fusion gene
            defines the majority of pilocytic astrocytomas
  JOURNAL   Cancer Res 68 (21), 8673-8677 (2008)
   PUBMED   18974108
REFERENCE   10 (bases 1 to 12498)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC083868.3, AM989469.1 and AC018663.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001164665.1.
            
            Summary: The protein encoded by this gene belongs to the UPF0606
            family. This gene has been found to be fused to the BRAF oncogene
            in many cases of pilocytic astrocytoma. The fusion results from 2Mb
            tandem duplications at 7q34. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2012].
            
            Transcript Variant: This variant (2), also known as the long-form,
            v2, represents the longer transcript and encodes the longer isoform
            (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.873509.1,
                                           SRR18074969.1184644.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000422774.2/ ENSP00000416040.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC083868.3         78429-78499         c
            72-6354             AM989469.1         1-6283
            6355-12498          AC018663.3         140683-146826       c
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q34"
     gene            1..12498
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="KIAA1549"
                     /db_xref="GeneID:57670"
                     /db_xref="HGNC:HGNC:22219"
                     /db_xref="MIM:613344"
     exon            1..307
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..5973
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="UPF0606 protein KIAA1549 isoform 2"
                     /protein_id="NP_001158137.1"
                     /db_xref="CCDS:CCDS56513.1"
                     /db_xref="GeneID:57670"
                     /db_xref="HGNC:HGNC:22219"
                     /db_xref="MIM:613344"
                     /translation="MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGL
                     LLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQ
                     HSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNF
                     LPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQ
                     PAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEG
                     VETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLE
                     ETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSE
                     LHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAET
                     LCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGIS
                     AEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILL
                     DSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFAS
                     SFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQ
                     LSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDS
                     EAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPI
                     LTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTGTVLITDAYLPSGSSFVSEATP
                     FPLPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSA
                     APPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIIT
                     AIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLI
                     LSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYN
                     LTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGY
                     PVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVS
                     TRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINK
                     MDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILY
                     WKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLP
                     GPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGA
                     VNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPI
                     PAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDD
                     LSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGC
                     PADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPF
                     PASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQ
                     EERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQ
                     QPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPP
                     SRGSQYGGPGWPSYGEDEAGRREATHMLGHQEYSSSPLFQVPRTSGREPSAPSGNLPH
                     RGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS"
     exon            308..2998
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3087
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3265
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3266..3396
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3397..3580
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3581..3640
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3641..3789
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3790..3967
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            3968..4152
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            4153..4349
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            4350..4465
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            4466..4671
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            4672..4895
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            4896..5049
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            5050..5367
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            5368..5414
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            5415..5572
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            5573..5718
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     exon            5719..12498
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6746..6751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="hexamer: AATACA"
     polyA_site      6771
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="major polyA site"
     regulatory      12473..12478
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="hexamer: AATAAA"
     polyA_site      12498
                     /gene="KIAA1549"
                     /gene_synonym="RP86"
                     /note="major polyA site"
ORIGIN      
        1 ctctccggcc tccctccgcg ccggtgcgcg gcgccccgtc ccagccgcag ccgcagcggc
       61 cgcccctccc ggacccgaga gccgctgagc cgcgaggccg ggccggggcg ccggccggga
      121 atgccggggg cgcggcgccg acgccgaggc gcggccatgg aggggaagcc ccgcgccggg
      181 gtcgcgctgg ccccggggcc gagcggccga cggccttccg cccgctgcgc ccgccgccgc
      241 cgcccggggc tgctgcttcc tggcctctgg ctgctgctgc tggcccggcc ggcctcgtgc
      301 gccccagatg agctctctcc ggaacagcac aacctttctt tatactccat ggagctcgtg
      361 ctgaagaaaa gcactgggca cagcgctgca caagtggcct taacagaaac tgctcccggc
      421 tcccagcaca gcagtcctct ccatgtcaca gccccgccgt ctgccactac ttttgataca
      481 gcctttttta accaaggaaa acagaccaaa agtacagcag atcccagcat ctttgtggca
      541 acttacgtgt cagtgacgag taaagaggtg gccgtcaatg acgatgagat ggataacttt
      601 ctgccagata ctcactggac cactccacgg atggtttctc caatacagta tatcacagtc
      661 agcccaccag ggctgcccag ggaagcatta gaacctatgc tcactccatc attacccatg
      721 gtttctttac aagatgaaga agtgacatcg ggctggcaga acacaacgcg acaaccagcg
      781 gcatatgctg agtccgccag tcatttccac acctttcggt cagcttttcg cacctctgag
      841 ggcatcgttc caactcctgg caggaatttg gtgctttatc ctactgatgc ttacagtcat
      901 ttatcaagca ggactctgcc agagattgtg gcttccctaa cagagggtgt ggaaaccacc
      961 ctttttttaa gctcccggtc tttaatgcca cagccgttag gcgacggcat tactataccg
     1021 ttgccctcct tgggggaggt ctcacagcct ccagaggagg tttgggccac aagtgcagac
     1081 agatacactg atgtgaccac tgtgttgagt caaagcctag aagaaaccat ctctccaaga
     1141 acatacccca ctgtgactgc atcgcacgca gcccttgcat tcagcaggac acattctcca
     1201 ttgctttcaa ctcctcttgc atttgcgtcc tctgcttcac caactgatgt ttcatctaac
     1261 ccctttctcc ctagcgactc cagcaaaaca tccgaattgc atagcaattc agccctcccc
     1321 ggtcctgtgg acaacactca tatcctgagc ccggtgtcct cattcagacc atacacttgg
     1381 tgtgcggcct gcactgtgcc ttcacctcag caagttctgg ccacgagcct catggagaaa
     1441 gacgtgggat caggggatgg tgccgagact ctgtgcatga ccgtgctgga agaaagcagc
     1501 atctctctaa tgagtagcgt cgtagcagac ttctctgaat ttgaggaaga tcctcaagta
     1561 tttaatacgc ttttcccctc cagacctatc gtcccacttt cttctagatc catggaaatc
     1621 tcagagacga gtgttggcat ttctgccgag gtggatatga gtagtgttac aaccacacag
     1681 gttccccctg cccacggccg cctctctgtg ccggcgtcac ttgatcctac tgctggctcc
     1741 ttgtctgttg ctgaaaccca agtgacgcca tccagcgtga ccactgcatt tttctcggtc
     1801 atcaccagca ttctccttga ctcatctttc tctgtcatag caaacaaaaa cacaccgtcg
     1861 cttgccgtca gagacccgag tgtttttacg ccttatagtc tggttccttc agtggagtct
     1921 tcacttttct ctgaccaaga acgttccagt ttttctgagc ataaacccag aggtgctttg
     1981 gattttgcat ccagcttttt ctcaacaccc ccgctggaac tcagcggctc catctcttcg
     2041 ccttcggaag cacctgcgtc tctgtctctg atgccgagtg acttgtcccc cttcacatct
     2101 cagtcttttt ctcccttggt tgagacattt acattgtttg actctagtga tctgcagtca
     2161 tctcagctgt ctcttcccag ttccacaaat cttgagtttt cgcagctcca gccaagttcc
     2221 gagctgcctt taaacaccat catgttgcta cctagccgtt ctgaggtgtc accatggtca
     2281 agcttccctt ctgattctct cgagtttgtt gaagcgtcta cggtttcact gacggattca
     2341 gaagctcatt ttacctcagc tttcattgaa actacctcct atcttgagtc ttcactcatt
     2401 tcccatgaat ccgcagtcac tgcactggtg ccccccggct ctgagtcttt tgacattttg
     2461 actgccggga ttcaagcaac atcaccattg accactgtcc acacaacgcc cattttaact
     2521 gagtcttctt tgttctcaac tctgacacct cctgacgacc aaatcagtgc tctagacggt
     2581 cacgtgtctg tcctggcctc tttctccaaa gccattccca ctggtacggt gttgatcact
     2641 gacgcgtacc tgccatcagg atcctcgttt gtttctgaag caaccccctt ccctctgccc
     2701 acagagctga ccgtcgtggg cccatcactc acacccacag aggtgccact gaacacctcc
     2761 acggaagtga gcacaaccag caccggtgct gccactggtg gtcccctcga ctccaccctg
     2821 atgggtgacg ccgcaagtca gagcccccca gagagtagtg ctgctcctcc cctgccatcc
     2881 ctgcgtcccg tgactgcctt cactctcgaa gcaacagtcg acacaccaac actggctact
     2941 gccaagccgc catatgtttg tgatatcaca gtccccgatg cctatctgat cacaactgtg
     3001 ctggccagaa gagctgtgca ggagtacatc attacagcaa tcaaagaagt actgaggatt
     3061 cacttcaacc gtgcagtgga actgaaggtt tacgaactat ttactgactt cacttttctg
     3121 gtaacatccg gtcctttcgt ttacacggca atatccgtca taaatgtgct tataaacagt
     3181 aagcttgtcc gtgaccagac tcctttaatc ctgtctgtga aaccttcttt ccttgtgcca
     3241 gagtccaggt tccaagttca aacagtactt cagtttgtgc ctccgagtgt ggatactggc
     3301 ttctgcaact tcacccagcg cattgagaaa ggcctaatga cagctctctt tgaagtgaga
     3361 aaacaccacc agggaacgta taacctcacg gtgcagatct tgaatatcac catcagttcc
     3421 tcaagggtga ctcctcggcg gggcccggtg aatatcatct ttgcggttaa aagcacacag
     3481 ggatttttga atgggtcgga agtgagcgag ctgctcagaa acttgagtgt ggtggagttc
     3541 agtttctatc tgggataccc agtgctgcag atcgcagagc ccttccagta tccacagctc
     3601 aacttatctc agttgctgaa gtcctcttgg gtcagaacag ttctcctggg cgtcatggag
     3661 aagcaactcc agaatgaagt gtttcaagcc gagatggaac gcaagctggc ccagctgctc
     3721 agcgaggttt ccaccagaag gcggatgtgg agaagggcca ctgtagctgc agggaacagt
     3781 gtggtgcagg tggtaaatgt gtcgaggctg gagggagatg acaatccggt acagctcatc
     3841 tactttgtgg aggatcaaga tggagaaaga ctcagtgcag tcaagtcttc ggacctgatt
     3901 aacaaaatgg acctccagag agcagccatc atcttgggtt accgaattca aggtgtcatt
     3961 gcccagcctg tcgacagggt gaagaggccg tctccggaat cccagagcaa caacttgtgg
     4021 gtcattgttg gcgtggtcat cccagtgctg gtggtgatgg tgattgttgt catcctctac
     4081 tggaaactat gccgcacaga caagctagac tttcagcctg acactgtggc caacattcag
     4141 cagcgtcaga agctgcagat ccctagtgtg aagggcttcg attttgctaa gcagcatctg
     4201 ggtcagcaca ataaagacga catattgatt attcatgagc cagcgccact gccaggacct
     4261 ctgaaggacc acaccacgcc ctcggaaaat ggagacgtgc caagccccaa gtcaaagatc
     4321 ccttccaaga atgttcgtca cagaggaaga gtttctccct cagatgctga ctctacggtc
     4381 agtgaagagt ccagcgagag ggacgcagga gataagacgc cgggagccgt caacgatggc
     4441 aggtcccaca gagctccgca gagcgggcca ccactgccca gttcgggaaa tgagcagcac
     4501 tcatcagcct ccatcttcga gcacgtggac aggatctccc gccccccgga ggctagccgg
     4561 cgggtcccca gtaagatcca gcttatcgcc atgcagccga tcccggcacc tcccgtccag
     4621 cgcccctccc cagccgaccg agtggcggaa agcaataaaa tcaacaaaga gattcagacc
     4681 gcgctgcggc acaagtctga gatcgagcac catcgcaaca agatccgcct gcgcgccaag
     4741 cgccgcgggc actacgagtt cccggtggta gacgacctgt cctcgggcga cactaaggag
     4801 cgacaccggg tgtaccgcag ggcacagatg cagatcgaca agatcctgga ccccacggcc
     4861 agcgtgccct ccgtgttcat agagcccagg aagagctcac ggataaaacg ttctcccaag
     4921 cctcgccgga aacaccaggt caacggctgt cctgccgacg ctgagaagga ccggctcatc
     4981 accacagaca gcgatggcac ctacaggagg ccccccggcg tccacaactc agcctacatc
     5041 ggatgcccat cggatcctga cctcccagcc gatgtgcaga caccatcctc ggtggaactg
     5101 gggaggtatc cagcccttcc cttcccggcc tcccagtaca tcccacccca gccgtccatc
     5161 gaggaggcac gccagaccat gcactccctc ctggacgacg cctttgccct cgtggccccc
     5221 agcagccagc ctgccagcac cgcaggtgta ggccccggag tcccacccgg cctgcccgca
     5281 aacagcaccc cttcccagga agagaggcga gccacccagt gggggtcctt ctacagccca
     5341 gcccagacgg ccaacaatcc ctgcagtaga tacgaagact atggaatgac tcccccgacg
     5401 ggtccattgc caagaccagg ttttggcccc ggtttgctgc agtctacaga gctggtgccc
     5461 cctgaccctc agcagccaca ggcctccgcc gaagccccat ttgctgccag agggatctac
     5521 tcggaggaga tgccgtcggt ggcccggcct cggcctgtcg ggggtaccac aggctcccag
     5581 atccagcacc tgacacaggt ggggattgcc agcagaattg gagctcagcc agtggaaatc
     5641 ccgccaagca gaggcagcca gtatgggggg ccaggctggc cttcgtacgg ggaggacgaa
     5701 gcggggcgaa gagaggccac acacatgctc ggacatcaag agtattcttc ttcaccgcta
     5761 tttcaggtgc caaggacttc aggcagggag ccctcagctc cttccgggaa cctcccccac
     5821 cggggactgc agggccctgg gctgggttac cccaccagct ccacggaaga cctccagcct
     5881 ggccactcct cggcctctct catcaaagca atccgcgagg agctcctccg gctctcccag
     5941 aaacagagca ccgtgcagaa cttccacagc tgatcggcct cgcctcgcag atttgccaag
     6001 tatccgcttc ctgtggaagc aagaccaaaa ggaaatcaac tgagtgggtg tttggaagag
     6061 gaaggagcaa ctctcgggca gcctgcccaa gggagggagc aagttgcaat ttagaagatg
     6121 ccatacgtcg tgtgacagct catgagcctt tcactgggct ggcaattgtc tgaacacttg
     6181 ggttcagttg aaatatatgt attttggcca aaagccagca gcacttcaca aaaacaaaac
     6241 acaaacctaa gctaacaaaa tgactgcatt cgtctctttt ttaaaggtag agattaaact
     6301 gtatagacag catagggatg aaaggaacca agcgtttctg tgggattgag actggtacgt
     6361 gtacgatgaa cctgctgctt tgttttctga gaagaggttt gaagacattt tattaacagc
     6421 ttaatttttc tcttttactc cataggaact tattttaata gtaacattaa caacaagaat
     6481 actaagactg tttgggaatt ttaaaaagct actagtgaga aaccaaatga taggttgtag
     6541 agcctgatga ctccaaacaa agccatcacc cgcattcttc ctccttcttc tggtgctaca
     6601 gctccaaggg cccttcacct tcatgtctga aatggaactt tggctttttc agtggaagaa
     6661 tatgttgaag gtttcatttt gttctagaaa aaaaaaatcc ctcccaaagt ggggcaaaaa
     6721 gctttatatt tatttgatta tccaaaatac agatcaaagt ttagatctac attcttcatt
     6781 gtatttgctg tttcttaatt gggcacacac aactcctggt catgtcccag ttcagcaccg
     6841 atcgctaagg ccgatcctgt agaatgcggc tttcaagagg tcctaactga tcctttcttc
     6901 cactttgctg ttgatttgtt cacaaattat gtctgaatga gaaatcttct gtaagcagaa
     6961 gttatttaat aattcccagc accacgaaat tgttatacat acatccctac cagtcacatt
     7021 ccctgtgttc agagcctgga aatgaaattg agttcagttc agcctctctg taatgaatca
     7081 agaaatgtaa aagagctttg agaccccaag ggaaaggaga gagttgcttc tcatttctga
     7141 ttttattgtg ctgttactct gtcgagtggc tattaaaatt gtcttatgct ctttgggcta
     7201 agaggggatc atctcgctta agatgtactc ctgatgtaaa catttccccc actttccaaa
     7261 taatggtaaa gaaaacagtg gcaagggctg ttctgttttc tggtacctga gtgaaactca
     7321 gaagaaaagc aggcttagct aagagatttt cactattaac ctgtttttat tagatcagcg
     7381 aagacagtca tgcatcgctt aatgatgggg atgcattctg agaaatgtgt cattaggctg
     7441 ttttgttgct gtgccaacat cctagattgt acttacacaa cctacatggt atagcctact
     7501 acacacctgg gctgtgtggc atggcctatt cctcttaggc tacaaacctg tacagcatgt
     7561 tactgtactg aacacaatgg taagtatttg tgtatctaaa catagctaaa catagaaaag
     7621 gtatggtaaa aatacaattt tataatctta tgggactacc atcaatatgt agaccgtcat
     7681 tgaccaaaac atcctgatgt gtgcatgact gtacttttca ttaaataaca gataaggggc
     7741 tatagaattt ggggctctga ctgatcaaaa cctgtgtatc tgtgtcaagt tttaagaccc
     7801 ctgaaacagc taaaacaggt ttccaaagtg taatcactgg atttcaaaac catgttttag
     7861 cccttcatta atgaagcctg ttgtacagat ttagagtctt acaatatgag ggaagttggt
     7921 tctgggaagg taagatgtag ccagttttca tctgggcacc tctgaaagag aaggagtgca
     7981 ggagagtaag tctctcaagg acgttcaaca aaggaccagc agcatcttat caggcgatcc
     8041 tcagaggctc caaggagcat tggagagaat gcccctttct gggcttgatg tcttggtttt
     8101 ggtttaatac caaatttctc ttggtcttgg tttaataccc gactcctccc aaattgaacg
     8161 attatctttg tatgtcactc aaaaatacca ggtttcctga atatgttatt aagaatttca
     8221 gatatccaag cagaatttta ttatggacac cttgtaatga attcaaatcg tgtgacagca
     8281 aacgggataa atggcccctt ctcacccagt tccgctcagg gccatacagg cttgcacata
     8341 gagtgtgcta aaaatggtaa cctcctttga gcattgcaga aagagggttc tgtttcaaat
     8401 tgggctgacc gtaaaagcaa ttttgatgtc tttcaaacta ccataaaggg attttaactg
     8461 tatttttatt cttagaaaca atacaccttt aaacagatgt caagaccaaa gatgatgtat
     8521 aataaacagc cggtggttat ggtgtggtgt gagcaggccc ccggatcacc agccctctgc
     8581 tggtggtcat aaagcacaca cagtttgtac ttgcttcctc tcgacgcctg ccacaacagt
     8641 tttgccagtc accaaactca gaaaaaacta gctgtgtgag cagcaccgta ccctagcggt
     8701 cttcagacat taagttcacg tcatccactg aaaggaaggg tccttgcgtt gacagcgtgt
     8761 ggctttggag tccgtttatg gaagcactct acaatgaaca gttgaatttt atgtctattc
     8821 ttttttccta ttttaaaagt tctagtggta acacaaactg taaatttgag tcagaatgtt
     8881 tggagaaatg ttgttttttt ttttttcaga gactactttg tcactcactg accatgtctg
     8941 gttccttctc tgaacttcat tctccccata agccaaacaa gaacagacct cccccgccac
     9001 ctcccaggca ccgtagttgt gagaatcaga tgtgatcatg tgtgcagatg tcctgtgagg
     9061 agggagcata aagcactgtg aaaatgtagc atgctgtcta tgtggacgcc ctaggatgag
     9121 tgatgtgaaa cgctgcacta ctgcggtgaa tgggttcaca catgggtaat gagcaaaacc
     9181 gaaagctcgg tgtgactcag tttcctcact cccattttgc tttaatttca cttccttcac
     9241 caattttccg attgcattta ttaagcatct ttctgctccc gactttctgg tgtctctggc
     9301 accaaataac ccagcagaca tgagccttgc cttctgagat tttagaatct aagatagcac
     9361 gagtgggtat agaagatgga agataccgat aaataacatg gtttaagtgt caataaaatt
     9421 cattcgaaga gatcaggcgc ggtggctcac gcctgtaatc ccagcatttt gggaggctga
     9481 ggcaggtgga tcacttgaga tcaagagctt aaaaccagcc caggcaacat ggcaaaaccc
     9541 cgtctctaca aaaaaataca aaaactagct gggtgtggtg gcgcatgcct gtagtcccag
     9601 ctgaggcacg ataattgctt gaacccgggg ggtggaggtt gcagtgagct gagattgcac
     9661 cactgtactc caacctgggc gacagagcaa gactgtgtct caaaaagaaa aaaatttgtt
     9721 caaaagacat aaatgaatta ggcacccaga agaagttgca tgtttggaaa tgcaatatct
     9781 tggggagacg tcaacttctg aagtgacttt gaaggcaggg cagctaccca ggcaaatggc
     9841 gtggaaggaa gagctgagag tgggcctggg aaaggctgta gggaaggagt gaagctgtca
     9901 ctgaaatgaa cttgatcttg atctgctttg atatggggac agaatccccg actgcactat
     9961 tgagggagtt tcagaagaga aggaagaggt gctggaagga tgcttttgca gtcatgcagc
    10021 aaggaaacga ccagggctgt gaagatctac agaggaagta ctccagtgtg ggggaggcaa
    10081 gggaagagga cgaaaatgat gagaatgaca ctgaggtcat tgtttagagc agatctcaag
    10141 cagccgtttg gccacgccat atcctttgtg gagcatagtg ggttatctat ctgtctgtct
    10201 gtctatgtat ctgtctatct atctatatat atattcagct tctttactgc tacctacaca
    10261 tttttctcca aattttatta atttcatagt gttttgattt gggtggcaga tgacttttaa
    10321 gcagtgggtg tttgccggcc agatctttcc tggcatgcgg actgtgaggc aaagcacggg
    10381 tgaaggtagg cgctaaaggc tttgggctaa agccagcacg cggttctgtg ctataggagt
    10441 ctcccgtttc ccgtggacag gttcagcgtt ccttctttcg cacaactttt ttctaagtgt
    10501 tccagtgacc aagccagtca ttcggacact gatttgcagt gcattggcag taattcacaa
    10561 attagttgtt atataagtct ctctcatccc tttcacacta gattctcaga catgaatgaa
    10621 tttgtcgttt ggaaggaaag ctgggtaacg ttttgggcac aggggaagga ggactccggt
    10681 cttaactccc acgctaactt tagctcaagt ggagttttca ccgtggtcat ttctacctcc
    10741 ggagcaaggt gccagcgcca gtactagagc ctgcttatcc acatttgccc tggacaggag
    10801 caggaggaag tccacttctg taccggcaga cagagcatgt gaacacaaaa cacatttcta
    10861 tggcatagtc aactgaactt catttttaca tttaatctaa catgttaaca cgttctaaca
    10921 gggtttctat gagcagctgc tgtaacatac tcatcaacta tgatagactt aacacttgtt
    10981 acctaatgaa caaggaggat gtgcatttcg ggtttctttt gatattcgtg gaggtgattg
    11041 tcagtgttta aacaggacta ctttctccac tatgaagatg acttggaaat cgcaacatca
    11101 tggtacattg ctaagtccat gcttgtgtgt gtgacagtag gcttgataat ttatcttaaa
    11161 acacagcagc attgaaattt aggaaaagaa tattaaatgc ctttggaaac atgaaacaaa
    11221 gttaggagcc agtaagaaag tgacagaagc aatgaatgtc ttaatgcagt atagttaaaa
    11281 tggcttccca cagggtagat aattatccag gatccttcct tttccttctt cctccaggtt
    11341 tttcaggggt cacttcacat ttctaaagaa agagtgaata ggctgggcat ggtggctcaa
    11401 gcctctaatc tcaacgcttt gggaggctga ggcaggagga tcgcttgagg ccaggagttt
    11461 gagaccacct tgggcaacat aacgagaccc cgtctctaca aaaaaaattt aaaaattagc
    11521 tgagcatggt ggcatgtgcc agtagtccca gctactcgaa aggctaagac tggaggatcg
    11581 cttgagccaa tgagttggag gctgcagtga gctataatca cgccactgca ctccagcctg
    11641 ggctgcaggg tgaggtcctg tctctggaaa aaaaaaaaaa aaaaaaagga ataggtaaaa
    11701 gggacagagg tgaaattttg agtgacttga gtctcttgca gtccctgatt acacagaacc
    11761 tttctgggct acttggagca tcacgaatag tctttcctgt acttaccaga tttcaagtat
    11821 tcataacttg actccctaag tgtacaagtt gggaatagta cagggccaag ttcaagtcgc
    11881 atatgctgta ctgttcctcc tgcaaatgtg gggaaagaag agggagatac tagaggaact
    11941 gaggctccac ccattcattc agttgctcta agcaccagag gacttgtttc agaaaagggg
    12001 agtgggaacg ccctcgactt tgccctcctc cggagcatct ctgggacgca gggagtctgg
    12061 ctagcgttaa taggaaaggt tgctcggcag agctgccctg gagtactgac ttgtctctcc
    12121 ctcctttgtc aaggtccatg tttttctggc tcttcctgca cactcatccc tagattatga
    12181 gcgttaatgt acgaaatgtg cagagcaaat tgaggccaac tgtggcatca atactgcaac
    12241 ttaaacttga caatcatggt ttgctggtcc tcaaacaggc actgaaatct cagtatgggt
    12301 atacgattta ataatcggcc cctcccttct atttgtcggt tttatgtttc tatccttcaa
    12361 tagctgcact gttttctaat gtgctgtaga gtttttgaaa taatttatgc aagtatttgg
    12421 tttccatgtt tacaatttat acagctttcc tagcatttta aatggaaatg tcaataaatg
    12481 ctatgaattg gtgtcaaa
//
