LOCUS       NM_001329943            8558 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens KIAA0586 (KIAA0586), transcript variant 7, mRNA.
ACCESSION   NM_001329943
VERSION     NM_001329943.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Zhao,Q., Xu,B., Xiang,Q., Tan,Y., Xie,H., Gao,Q., Wen,L., Wang,H.,
            Yang,M. and Liu,S.
  TITLE     Compound heterozygous splicing variants in KIAA0586 cause fetal
            short-rib thoracic dysplasia and cerebellar malformation: Use of
            exome sequencing in prenatal diagnosis
  JOURNAL   Mol Genet Genomic Med 11 (3), e2124 (2023)
   PUBMED   36538006
  REMARK    GeneRIF: Compound heterozygous splicing variants in KIAA0586 cause
            fetal short-rib thoracic dysplasia and cerebellar malformation: Use
            of exome sequencing in prenatal diagnosis.
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Wang,J., Li,T., Wang,J.L., Xu,Z., Meng,W. and Wu,Q.F.
  TITLE     Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor
            Delamination to Sustain Neurogenesis by Stabilizing Adherens
            Junctions
  JOURNAL   Cell Rep 33 (11), 108495 (2020)
   PUBMED   33326788
  REMARK    GeneRIF: Talpid3-Mediated Centrosome Integrity Restrains Neural
            Progenitor Delamination to Sustain Neurogenesis by Stabilizing
            Adherens Junctions.
REFERENCE   3  (bases 1 to 8558)
  AUTHORS   Sumathipala,D., Stromme,P., Gilissen,C., Einarsen,I.H.,
            Bjorndalen,H.J., Server,A., Corominas,J., Hassel,B., Fannemel,M.,
            Misceo,D. and Frengen,E.
  TITLE     Sudden death in epilepsy and ectopic neurohypophysis in Joubert
            syndrome 23 diagnosed using SNVs/indels and structural variants
            pipelines on WGS data: a case report
  JOURNAL   BMC Med Genet 21 (1), 96 (2020)
   PUBMED   32381069
  REMARK    GeneRIF: This lead to the identification of the most common variant
            detected in patients with JBTS23 (OMIM# 616490), rs534542684, in
            compound heterozygosity with a 8.3 kb deletion in KIAA0586, not
            previously reported.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8558)
  AUTHORS   Fraser,A.M. and Davey,M.G.
  TITLE     TALPID3 in Joubert syndrome and related ciliopathy disorders
  JOURNAL   Curr Opin Genet Dev 56, 41-48 (2019)
   PUBMED   31326647
  REMARK    GeneRIF: Loss of KIAA0586 protein (TALPID3) function can cause both
            severe lethal and mild cilia-related developmental disorders
            [Review]
            Review article
REFERENCE   5  (bases 1 to 8558)
  AUTHORS   Tsai,J.J., Hsu,W.B., Liu,J.H., Chang,C.W. and Tang,T.K.
  TITLE     CEP120 interacts with C2CD3 and Talpid3 and is required for
            centriole appendage assembly and ciliogenesis
  JOURNAL   Sci Rep 9 (1), 6037 (2019)
   PUBMED   30988386
  REMARK    GeneRIF: CEP120 interacts with C2CD3 and Talpid3 and is required
            for centriole appendage assembly and ciliogenesis.
            Erratum:[Sci Rep. 2020 Jan 22;10(1):1265. doi:
            10.1038/s41598-020-58151-y.. PMID: 31965052]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8558)
  AUTHORS   Kobayashi,T., Kim,S., Lin,Y.C., Inoue,T. and Dynlacht,B.D.
  TITLE     The CP110-interacting proteins Talpid3 and Cep290 play overlapping
            and distinct roles in cilia assembly
  JOURNAL   J Cell Biol 204 (2), 215-229 (2014)
   PUBMED   24421332
  REMARK    GeneRIF: Talpid3 and Cep290 play overlapping and distinct roles in
            ciliary vesicle formation through regulation of centriolar
            satellite accretion and Rab8a
REFERENCE   7  (bases 1 to 8558)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   8  (bases 1 to 8558)
  AUTHORS   Yin,Y., Bangs,F., Paton,I.R., Prescott,A., James,J., Davey,M.G.,
            Whitley,P., Genikhovich,G., Technau,U., Burt,D.W. and Tickle,C.
  TITLE     The Talpid3 gene (KIAA0586) encodes a centrosomal protein that is
            essential for primary cilia formation
  JOURNAL   Development 136 (4), 655-664 (2009)
   PUBMED   19144723
REFERENCE   9  (bases 1 to 8558)
  AUTHORS   Davey,M.G., Paton,I.R., Yin,Y., Schmidt,M., Bangs,F.K.,
            Morrice,D.R., Smith,T.G., Buxton,P., Stamataki,D., Tanaka,M.,
            Munsterberg,A.E., Briscoe,J., Tickle,C. and Burt,D.W.
  TITLE     The chicken talpid3 gene encodes a novel protein essential for
            Hedgehog signaling
  JOURNAL   Genes Dev 20 (10), 1365-1377 (2006)
   PUBMED   16702409
  REMARK    GeneRIF: The chicken ortholog functions in regulation of the Gli
            repressor and activator proteins in the Hedgehog signaling pathway.
REFERENCE   10 (bases 1 to 8558)
  AUTHORS   Parisi,M. and Glass,I.
  TITLE     Joubert Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139021.6 and AL135752.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001329943.2.
            
            Summary: This gene encodes a conserved centrosomal protein that
            functions in ciliogenesis and responds to hedgehog signaling.
            Mutations in this gene causes Joubert syndrome 23. Alternative
            splicing results in multiple transcript variants and protein
            isoforms. [provided by RefSeq, Aug 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.181777.1,
                                           SRR1660803.157332.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000652326.2/ ENSP00000498929.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-745               AL139021.6         91365-92109
            746-816             AL139021.6         93009-93079
            817-886             AL139021.6         94294-94363
            887-956             AL139021.6         96034-96103
            957-1131            AL139021.6         106352-106526
            1132-1353           AL139021.6         107600-107821
            1354-1507           AL139021.6         111986-112139
            1508-1675           AL139021.6         114225-114392
            1676-1799           AL139021.6         116996-117119
            1800-1908           AL139021.6         120348-120456
            1909-2129           AL139021.6         121405-121625
            2130-2202           AL139021.6         122119-122191
            2203-2430           AL139021.6         123489-123716
            2431-2605           AL139021.6         124632-124806
            2606-2800           AL139021.6         129481-129675
            2801-2988           AL139021.6         131381-131568
            2989-3099           AL139021.6         134259-134369
            3100-3180           AL139021.6         135845-135925
            3181-3371           AL139021.6         138253-138443
            3372-3490           AL139021.6         140769-140887
            3491-3690           AL139021.6         146159-146358
            3691-3850           AL139021.6         150653-150812
            3851-4073           AL139021.6         151533-151755
            4074-4327           AL139021.6         152267-152520
            4328-4404           AL139021.6         153810-153886
            4405-4536           AL139021.6         155790-155921
            4537-4714           AL139021.6         162429-162606
            4715-4869           AL139021.6         172201-172355
            4870-4975           AL135752.6         97499-97604         c
            4976-5041           AL135752.6         69990-70055         c
            5042-8558           AL135752.6         58829-62345         c
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.1"
     gene            1..8558
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="KIAA0586"
                     /db_xref="GeneID:9786"
                     /db_xref="HGNC:HGNC:19960"
                     /db_xref="MIM:610178"
     exon            1..745
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     CDS             547..5193
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="isoform 7 is encoded by transcript variant 7;
                     protein TALPID3"
                     /codon_start=1
                     /product="protein TALPID3 isoform 7"
                     /protein_id="NP_001316872.1"
                     /db_xref="CCDS:CCDS91882.1"
                     /db_xref="GeneID:9786"
                     /db_xref="HGNC:HGNC:19960"
                     /db_xref="MIM:610178"
                     /translation="MKGSEVSLEKKKKIKMPVKRLREVVSQNHGDHLVLLKDELPCVP
                     PALSANKRLPVGTGTSLNGTSRGSSDLTSARNCYQPLLENPMVSESDFSKDVAVQVLP
                     LDKIEENNKQKANDIFISQYTMGQKDALRTVLKQKAQSMPVFKEVKVHLLEDAGIEKD
                     AVTQETRISPSGIDSATTVAAATAAAIATAAPLIKVQSDLEAKVNSVTELLSKLQETD
                     KHLQRVTEQQTSIQRKQEKLHCHDHEKQMNVFMEQHIRHLEKLQQQQIDIQTHFISAA
                     LKTSSFQPVSMPSSRAVEKYSVKPEHPNLGSCNPSLYNTFASKQAPLKEVEDTSFDKQ
                     KSPLETPAPRRFAPVPVSRDDELSKRENLLEEKENMEVSCHRGNVRLLEQILNNNDSL
                     TRKSESSNTTSLTRSKIGWTPEKTNRFPSCEELETTKVTMQKSDDVLHDLGQKEKETN
                     SMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKILRGVQNNKKVLEENLEAI
                     IRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQDELSRTDYEQKRFD
                     QKNQRTKKGQNMTKDIRTNTQDKTVNKSVIPRKHSQKQIEEHFRNLPMRGMPASSLQK
                     ERKEGLLKATTVIQDEDYMLQVYGKPVYQGHRSTLKKGPYLRFNSPSPKSRPQRPKVI
                     ERVKGTKVKSIRTQTDFYATKPKKMDSKMKHSVPVLPHGDQQYLFSPSREMPTFSGTL
                     EGHLIPMAILLGQTQSNSDTMPPAGVIVSKPHPVTVTTSIPPSSRKVETGVKKPNIAI
                     VEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKEASLPPVQTWIKTPEIM
                     KVDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPPFPPV
                     ASTFQPTADILDKVIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSE
                     TSEPLTSDIVEGTSSGALQLFVDAGVPVNSNVIKHFVNEALAETIAVMLGDREAKKQG
                     PVATGVSGDASTNETYLPARVCTPLPTPQPTPPCSPSSPAKECVLVKTPDSSPCDSDH
                     DMAFPVKEICAEKGDDMPAIMLVNTPTVTPTTTPPPAAAVFTPTLSDISIDKLKVSSP
                     ELPKPWGDGDLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMDFPAQPPPPEPVPFM
                     PFPAGTKAPSPSQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKIL
                     EDIGLYLTNLNDSLSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESA
                     VSQQAVYHSEDLENSVGELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQQCDP
                     KPLSRQFDTVSGSIYEDSCASHGPMSLGELELEPNSKLVLPTTLLTAQENDVNLPVAA
                     EDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQVSPGDMDRTQIELNPYLT
                     CVFSGGKAVPLSASQMPPAKMSVMLPSVNLEDCSQSLSLSTMQEDMESSGADTF"
     exon            746..816
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            817..886
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            887..956
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1131
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1353
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1507
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1675
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1799
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1908
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..2129
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2202
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2430
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2605
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2800
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2988
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3099
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3180
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3181..3371
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3372..3490
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3690
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3691..3850
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            3851..4073
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4074..4327
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4328..4404
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4405..4536
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4537..4714
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4715..4869
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4870..4975
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            4976..5041
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     exon            5042..8558
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5361..5366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="hexamer: AATAAA"
     polyA_site      5381
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="major polyA site"
     regulatory      6054..6059
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="hexamer: ATTAAA"
     regulatory      6064..6069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="hexamer: AATAAA"
     polyA_site      6074
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
     polyA_site      6090
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
     regulatory      8469..8474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="hexamer: AATAAA"
     polyA_site      8499
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
     regulatory      8531..8536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
                     /note="hexamer: AATAAA"
     polyA_site      8558
                     /gene="KIAA0586"
                     /gene_synonym="JBTS23; SRTD14; Talpid3"
ORIGIN      
        1 gctgttgtca gattacaccc acgacggtgc gggtctcggg cgttctggag atacgtaggg
       61 gtgaatttat gtttccgacg attgcatctg gaggggtgtg taagactctg tggctgaaga
      121 aagctattac gcttcttatg tgggtcatta ttttaaaaat agcatttcgc ttttatttgc
      181 ttgactgcct cttctcaaca aattttaagc ccgccactac atgtgtttgg ttttgcccta
      241 ccagctctgg ggttggggag tgcactgtta tggttattgt tgctcctgtg gccattctct
      301 tgtcatcccc actttcacat tttggcgtgg tgtattaata gactgagtgg gattaatggg
      361 taaatatgac tttatagtca gctctaatcc tggattcaat atcagaattt agattttcag
      421 ctttgtggat gttcgacatt ttaaaaacag ttattgcaaa gaggtgaaaa ttttgttctg
      481 aagtcttaag gaaacagaga aagtttttcc gtccttaagt gtgggacttg ttttgtgacc
      541 aacaatatga aaggctctga ggtcagcttg gagaagaaaa aaaagattaa gatgccagtg
      601 aagagacttc gtgaggtagt ttctcaaaat catggagatc atttggtttt gctgaaagat
      661 gagttgccct gtgttcctcc ggcattgtct gcaaataaac gtcttcctgt tggaacgggg
      721 actagtttga atggaacatc acgtggttca tcagacttaa cttctgctag aaattgttac
      781 cagcctctat tagaaaatcc catggtgtca gaaagtgatt tttctaaaga cgttgcagtg
      841 caagtgttgc ctttggataa aatagaagag aacaacaagc aaaaagcaaa tgacatcttc
      901 atttctcagt atacaatggg acagaaagat gctctaagaa cagttttaaa gcaaaaagct
      961 caaagcatgc ctgtttttaa ggaagtaaag gtacatctgt tagaagatgc aggcatagag
     1021 aaggatgctg ttactcagga gactagaatt tcacccagtg gaattgattc agctacaacc
     1081 gtggctgcag caactgctgc tgccattgca accgcagctc cgttgataaa ggtgcagagt
     1141 gatttggaag caaaagtcaa ttctgttaca gaattactta gtaaattaca ggagactgat
     1201 aaacacctgc aacgtgttac agagcagcaa acaagcattc agaggaaaca agagaaatta
     1261 cattgtcatg atcacgaaaa gcaaatgaat gtgtttatgg agcagcacat aaggcatctt
     1321 gaaaagttac aacaacaaca aatagatatt cagactcatt ttattagtgc tgcactcaag
     1381 actagtagtt ttcagcctgt tagtatgccc tcctccagag cagtggaaaa gtattccgta
     1441 aaaccagaac accctaatct tggtagctgt aatccatctt tatataacac atttgcttcc
     1501 aaacaagcac ctttaaaaga agttgaagat acgagttttg ataaacagaa atctcctttg
     1561 gagacaccag cacctcgcag atttgctcct gtacctgttt caagggatga tgaactatca
     1621 aagagggaaa atcttttgga agaaaaagaa aatatggaag tgtcgtgtca cagaggaaat
     1681 gtaagactat tggaacaaat tttgaataat aatgattctt tgacaagaaa aagtgaatca
     1741 tcaaacacca cctcactaac taggtcaaaa ataggatgga ctcctgagaa aacaaacaga
     1801 tttccttcct gtgaagagct agaaacaact aaagtgacta tgcagaagtc tgatgatgtt
     1861 cttcatgacc ttggccaaaa agagaaagaa acaaatagca tggtccagcc aaaagaatct
     1921 ctgagtatgt tgaagcttcc agatcttcca cagaattctg ttaagcttca aacaaccaat
     1981 acaacaagat ctgtattgaa agatgctgag aagattttga gaggagtaca aaacaataaa
     2041 aaagtacttg aagaaaacct ggaagctatt attcgtgcaa aagatggagc tgccatgtat
     2101 tcgcttatca atgctttatc taccaacaga gagatgtcag agaaaattag gatcagaaag
     2161 acagtggatg aatggattaa aactatttct gcagaaattc aggatgaact gtcaagaaca
     2221 gattatgaac aaaaaagatt tgatcagaag aatcagagaa ccaagaaagg tcagaatatg
     2281 actaaagata ttagaaccaa cacacaagat aaaactgtca acaaatctgt aattccaaga
     2341 aaacattctc aaaagcaaat agaagagcat tttagaaatc tacctatgag gggcatgcct
     2401 gcttcaagtt tacagaaaga gagaaaggaa gggcttttga aagcaaccac agtaatacaa
     2461 gatgaagatt atatgttaca agtctatgga aagccagttt atcagggcca tcgaagcact
     2521 cttaaaaaag gaccatatct cagatttaat tctccatctc ctaagtccag accacagaga
     2581 ccaaaagtaa tagaacgagt taaaggcact aaggtaaagt caataagaac acagactgac
     2641 ttctatgcaa caaaacctaa gaagatggat tctaaaatga aacattctgt tcctgtgtta
     2701 cctcatggcg atcagcaata tttgttcagc ccaagtagag aaatgcctac tttttcaggt
     2761 acattggaag gtcatctgat tcctatggca attcttttag gacaaaccca aagtaatagt
     2821 gataccatgc cacctgctgg agtgattgtc agcaagccac accctgtaac tgtgactact
     2881 tctattcctc catcatctcg aaaagtagaa actggagtaa agaaacctaa catagccatt
     2941 gtagaaatga agtcagaaaa aaaggatcct cctcagctta ctgtgcaggt attacccagt
     3001 gtagatattg acagcatttc aaatagtagt gctgatgtcc tttcacctct gtctagcccc
     3061 aaagaagcat ctcttcctcc tgtgcaaact tggataaaga ctccagaaat tatgaaggta
     3121 gatgaagaag aggtgaagtt tccaggaact aactttgatg aaataatcga tgtcatacag
     3181 gaagaagaaa aatgtgatga aattccagac tctgaaccaa ttctggagtt taacagaagt
     3241 gttaaagctg attctacaaa atataatggt cctccatttc cgccagttgc ttctactttt
     3301 cagcccactg ctgatattct ggataaagta attgagagaa aagaaacact ggaaaatagc
     3361 ttaattcaat gggtagagca agaaataatg tcaagaatta tctctgggct ctttccagtc
     3421 cagcaacaga ttgcacctag tatcagtgtt tcagtcagtg agacaagtga accactgact
     3481 tctgacattg tggaaggaac aagcagtggc gccctccagc tttttgttga tgctggtgtt
     3541 cctgtgaact caaatgtgat taaacatttt gttaacgaag ctcttgctga gaccattgct
     3601 gtcatgctgg gtgacagaga agcaaagaag caaggtcctg ttgctacagg tgtttctggg
     3661 gatgcttcaa caaatgaaac atatttgccg gcaagagtgt gcaccccact gcctacccca
     3721 cagcctacgc ctccttgctc accttcatca cctgctaagg agtgtgtttt ggtaaagact
     3781 ccagattctt ctccctgtga ttcggatcat gatatggctt ttcctgtgaa agaaatatgt
     3841 gctgaaaaag gagatgatat gcctgccatc atgcttgtta atactccaac agttacccct
     3901 actactacac ctcctccagc ggcggcagtt tttaccccaa ctttgtcaga tatttccatt
     3961 gataaattga aggtatcaag cccagagctt cccaagccat ggggtgatgg agacctgcca
     4021 ctggaagaag agaaccctaa ctcacctcaa gaagaacttc atccaagagc tattgtaatg
     4081 tctgtggcta aggatgaaga accagagagt atggatttcc ctgctcagcc tccacctcca
     4141 gagccagttc cctttatgcc atttcctgcc ggcaccaagg ccccttcccc ctcacagatg
     4201 ccaggttctg attcatcaac actggagagc acattgagtg ttactgtcac tgaaactgaa
     4261 actttagata aacccatctc tgaaggagag attttattta gctgtggtca aaaattggcc
     4321 cccaagattt tagaagatat aggactgtac ctgacaaacc ttaatgatag cttatccagc
     4381 actctgcatg atgccgttga aatggaggat gatcctccta gtgaagggca agtgattagg
     4441 atgtcccata aaaaatttca tgcagatgca attctttctt ttgctaaaca aaaccaggag
     4501 tcagcagttt cccagcaagc agtctatcat tcagaggact tggaaaacag tgtgggtgaa
     4561 cttagtgaag gacaaagacc ccagctaaca gcggcagcag agaacatctt aatgggacat
     4621 tctctctata tgcagccacc tgtcactaat acacagtctt tggatcaaca atgtgatcct
     4681 aaaccattat ctcggcaatt tgacacagtt tcaggtagta tttatgaaga ttcatgtgct
     4741 agtcatggtc caatgagttt gggagaattg gagttggagc caaattctaa gctggttctt
     4801 cccacaacac ttctgacagc acaagaaaat gatgttaatt taccagtagc cgctgaagat
     4861 ttttcccagt accaactaaa gcaaaatcag gatgttaagc aagttgaaca caaaccatca
     4921 caaagttacc tacgtgttag aaataaatct gatattgcac cttcacagca acaagtttca
     4981 ccaggtgata tggatcggac acaaattgag cttaatccgt acctcacatg tgtattttca
     5041 ggtgggaaag cagtgccact ctccgcttca cagatgcccc ctgccaagat gtcagtgatg
     5101 ctgccgtcag tgaacctcga ggactgctct cagtctctga gtctcagcac aatgcaggag
     5161 gacatggagt cttcgggggc agataccttc tgaacgggaa gagacagcca gcacagtgtt
     5221 tatgccactg gttttaaagt cattttacct tggcttaaaa ccctctctca gactgtttgg
     5281 tttttgagca tattctgaaa aaaaaattcc aatattttaa aataaaacaa aaagcataat
     5341 ttgggtattt aaagttttta aataaaataa gtataagtca ttataagtct gattgtgagt
     5401 tgtttcttag aatcctgaga tagcatagag tagtgaaagg gcatggcttt tgacatctag
     5461 gcatttaatc tataggtgta attatctgta tgtatttata aaattatgta gctcaaggtt
     5521 attcattcta acatttttat aacagcaaat agaaaaaatc aaatgtctat caataataga
     5581 ctggttttaa agtgatggta catcagtaca atggaatact atgtattaca aaaaaaaatt
     5641 taaaaagttt attgatatgg aataatcttt gagatgcact gttaaagtga aaaaagccat
     5701 gtgcaaaata gtgtgtgaac tatgcagttg ttgaaattat ttaaaatggg gtggggagga
     5761 attcaaatgt atggtatgtc acatattctt acatgtattt gcttgtaaat atattatata
     5821 aacaagaaac tgctaagttt gtctccaggg agtagaattg ttggctgggt gataggagag
     5881 gctaagtgat aggaatcaag gataatcgtg gttactatgt atttgaactt cagttgtgat
     5941 agaaaaaaat catcagtgag attaattttt atatgcctta tgtataccat ataaaccttt
     6001 ataaacctgt gaaacatgta actctattac cttttgcaac ataaatacat aaaattaaag
     6061 gaaaataaaa agcattgctt cgtaatcaga tacatgtagg ttctaatccc aattctgtaa
     6121 cttcttgctt tgtgataggg gccaacgaaa cgccttctta tcagctgttt cagattaatg
     6181 ggtttaatac ttatttttgc agaataatgg acatcagtga ttgtgtgtgg agatgggggt
     6241 gggtagtgcc cagcacagtg cttgatacca tgtaagtgct caataaatgg tgttgttctt
     6301 attatcttga gggatttcag tactgtatag tagagggcat aagcacatga aaagaaaggc
     6361 agcctagcac tagtgaaaaa aatttacctc ccagaaaaat cattttctgt cacaactgaa
     6421 tttcaaacac acagtaatta cagtcatcct tgactttttc agcatttttg taggttgcta
     6481 agtggatctc agcactggca gcacattaga acctctggga gctttaaaaa atactaatgc
     6541 ctgggccttg taccaaggga tttcaattta attgatcttg gatggggtcc agcattggta
     6601 ttaaaaaaaa aaaaagacct tttcaggtaa ttttaatggg cactcaaggc tgagaattac
     6661 tgaagagcaa tttagaattc cttaaacttc tagtaggagc cattgcctgg ggattttttt
     6721 tctcactgaa catagaagct gcaagagtct atggaaactc agaaatgtgt cagccttcca
     6781 catgcacttc ctcacagctt ctctcaggta tttggttact ggaatgtttt aggtttcact
     6841 ttagaacccg ttttctttga tatggccctt tggacagttc tgcttataac agtttccttt
     6901 aaacaagtgg aagaaaaatt ctgctctgtg gattttaatt tgtttttgat attcatctac
     6961 aagactctgg gcaaggtgaa agctggagaa atgttgctta gtgctgcttc attttaaaca
     7021 tgacacccca ccccccaacc ttggtttctg gttttcatag ttttttctgc cttggaagga
     7081 tttcagcaaa atcttgtttt agttaaagca ttttttattt aaaggtgttg gtagattttt
     7141 cctcctaacc atagaaagcc tgccttttct aactttaggg tagaagtgta atctttgatt
     7201 tattctactc cctgtgcacg tgagcttttc ttttaaaaaa aattagtgtc cattcgacgg
     7261 tttctttttt ttttttttga gatggaattt tgctcgttgc ccaggctgga gtgcaatggc
     7321 acaatcttgg ctcaccgcaa cctctgcctc ctggtttcaa gcgattctcc tgccttagcc
     7381 tccttagtag ctgggattac aggcgtgcgc caccatgccc ggcaaatttt gtatttttag
     7441 tagagatggg gtttctccgt gttggtcagg ctagtcctga actcccgacc tcaggtgatc
     7501 tgcctgcctc agcctcccaa agtgctggga ttacaggcgt gagccaccgc gcccggcatt
     7561 catctagtat tttgttttat aggattattt ctgttcatgg ttactcctgt acacattcct
     7621 ctgccaacca cttcagattc ttttggaata agacgatgta cgtaaaaaca aactaaatgg
     7681 aacttttgat tttataagct ttaaaatttt tattttatat taaattttac aatttccaac
     7741 ttccggaata aactatttat gagacatgct attaagagac tatcaagatc aaggctgtta
     7801 ctattgctgg gctgtgactg gacacggtgg ctcatgcctg taatcccaag actttgggag
     7861 gccgagggtg gatcacttga gctcaggagt ttgagaccag cctgggcaac atggcaaaac
     7921 ccatctctac caaaaataca aaaaattagc tggtcatggt tgcacacacc tgtagcccca
     7981 gctactcaag agggtgaggt gagaggatcg cttgagccca ggatgtcaag actgcagtga
     8041 gccgtggttg tgccactgca ctccagcctg ggtgacagaa tgagaccgtg tctcaaaaaa
     8101 aaaaaaaaaa aaattgctga gctatcatgc cttaacacta ggtaacgtgt ttaaaatgtt
     8161 cctttctgct gggtgcgggt ggctcacacc tgtaatccca gcactttggg aggctgaggc
     8221 aggcagatca cccaaggtta ggagttcaag accagtctgg ccaacatggt gaaaccccat
     8281 ctctactaaa aatacaaaaa ttagccaggc atggtggcgt gtgcctgtaa tcccagctac
     8341 ttgggaggct gaggcaggag aattgcttca acccaggagg cagaggtggc aacgagctga
     8401 gactgcacca cttaactcca gcctgggcaa cagagtaaga ctctgtctca aaaataaata
     8461 aataaataaa taaaatgttc ctctgtttta aatcctaaat tcgttctttg tatactttaa
     8521 tagaatcttg aataaatatt tttattctta gaagatta
//
