LOCUS       NM_203500               2565 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens kelch like ECH associated protein 1 (KEAP1),
            transcript variant 1, mRNA.
ACCESSION   NM_203500
VERSION     NM_203500.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Dewanjee,S., Bhattacharya,H., Bhattacharyya,C., Chakraborty,P.,
            Fleishman,J., Alexiou,A., Papadakis,M. and Jha,S.K.
  TITLE     Nrf2/Keap1/ARE regulation by plant secondary metabolites: a new
            horizon in brain tumor management
  JOURNAL   Cell Commun Signal 22 (1), 497 (2024)
   PUBMED   39407193
  REMARK    GeneRIF: Nrf2/Keap1/ARE regulation by plant secondary metabolites:
            a new horizon in brain tumor management.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Li,L., Xie,D., Yu,S., Ma,M., Fan,K., Chen,J., Xiu,M., Xie,K., Li,Y.
            and Gao,Y.
  TITLE     WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to Enhance
            the Oxidative Stress Response in Hepatocellular Carcinoma
  JOURNAL   Cancer Res 84 (17), 2776-2791 (2024)
   PUBMED   38885324
  REMARK    GeneRIF: WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to
            Enhance the Oxidative Stress Response in Hepatocellular Carcinoma.
REFERENCE   3  (bases 1 to 2565)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   4  (bases 1 to 2565)
  AUTHORS   Namani,A., Veeraiyan,D. and Patra,T.
  TITLE     A comparative analysis indicates SLC7A11 expression regulate the
            prognostic value of KEAP1-NFE2L2-CUL3 mutations in human uterine
            corpus endometrial carcinoma
  JOURNAL   Free Radic Biol Med 222, 223-228 (2024)
   PUBMED   38876457
  REMARK    GeneRIF: A comparative analysis indicates SLC7A11 expression
            regulate the prognostic value of KEAP1-NFE2L2-CUL3 mutations in
            human uterine corpus endometrial carcinoma.
REFERENCE   5  (bases 1 to 2565)
  AUTHORS   Han,B., Zhen,F., Sun,Y., Sun,B., Wang,H.Y., Liu,W., Huang,J.,
            Liang,X., Wang,Y.R., Chen,X.S., Li,S.J. and Hu,J.
  TITLE     Tumor suppressor KEAP1 promotes HSPA9 degradation, controlling
            mitochondrial biogenesis in breast cancer
  JOURNAL   Cell Rep 43 (7), 114507 (2024)
   PUBMED   39003742
  REMARK    GeneRIF: Tumor suppressor KEAP1 promotes HSPA9 degradation,
            controlling mitochondrial biogenesis in breast cancer.
REFERENCE   6  (bases 1 to 2565)
  AUTHORS   Zipper,L.M. and Mulcahy,R.T.
  TITLE     The Keap1 BTB/POZ dimerization function is required to sequester
            Nrf2 in cytoplasm
  JOURNAL   J Biol Chem 277 (39), 36544-36552 (2002)
   PUBMED   12145307
  REMARK    GeneRIF: Keap1 dimerization causes Nrf2 sequestration
REFERENCE   7  (bases 1 to 2565)
  AUTHORS   Sekhar,K.R., Spitz,D.R., Harris,S., Nguyen,T.T., Meredith,M.J.,
            Holt,J.T., Gius,D., Marnett,L.J., Summar,M.L. and Freeman,M.L.
  TITLE     Redox-sensitive interaction between KIAA0132 and Nrf2 mediates
            indomethacin-induced expression of gamma-glutamylcysteine
            synthetase
  JOURNAL   Free Radic Biol Med 32 (7), 650-662 (2002)
   PUBMED   11909699
  REMARK    Erratum:[Free Radic Biol Med 2002 Jul 31;33(1):149. Guis David
            [corrected to Gius David]]
REFERENCE   8  (bases 1 to 2565)
  AUTHORS   Velichkova,M., Guttman,J., Warren,C., Eng,L., Kline,K., Vogl,A.W.
            and Hasson,T.
  TITLE     A human homologue of Drosophila kelch associates with myosin-VIIa
            in specialized adhesion junctions
  JOURNAL   Cell Motil Cytoskeleton 51 (3), 147-164 (2002)
   PUBMED   11921171
  REMARK    GeneRIF: In epithelial cells derived from retina and inner ear,
            Keap1 is a component of focal adhesions and zipper junctions.
REFERENCE   9  (bases 1 to 2565)
  AUTHORS   Dhakshinamoorthy,S. and Jaiswal,A.K.
  TITLE     Functional characterization and role of INrf2 in antioxidant
            response element-mediated expression and antioxidant induction of
            NAD(P)H:quinone oxidoreductase1 gene
  JOURNAL   Oncogene 20 (29), 3906-3917 (2001)
   PUBMED   11439354
REFERENCE   10 (bases 1 to 2565)
  AUTHORS   Itoh,K., Wakabayashi,N., Katoh,Y., Ishii,T., Igarashi,K.,
            Engel,J.D. and Yamamoto,M.
  TITLE     Keap1 represses nuclear activation of antioxidant responsive
            elements by Nrf2 through binding to the amino-terminal Neh2 domain
  JOURNAL   Genes Dev 13 (1), 76-86 (1999)
   PUBMED   9887101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK056204.1 and BC015945.1.
            
            On Nov 22, 2018 this sequence version replaced NM_203500.1.
            
            Summary: This gene encodes a protein containing KELCH-1 like
            domains, as well as a BTB/POZ domain. Kelch-like ECH-associated
            protein 1 interacts with NF-E2-related factor 2 in a
            redox-sensitive manner and the dissociation of the proteins in the
            cytoplasm is followed by transportation of NF-E2-related factor 2
            to the nucleus. This interaction results in the expression of the
            catalytic subunit of gamma-glutamylcysteine synthetase. Two
            alternatively spliced transcript variants encoding the same isoform
            have been found for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.603804.1, AK056204.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000171111.10/ ENSP00000171111.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1575              AK056204.1         212-1786
            1576-2565           BC015945.1         1454-2443
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..2565
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /note="kelch like ECH associated protein 1"
                     /db_xref="GeneID:9817"
                     /db_xref="HGNC:HGNC:23177"
                     /db_xref="MIM:606016"
     exon            1..116
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     exon            117..802
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..2038
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /note="cytosolic inhibitor of Nrf2; kelch-like protein 19;
                     kelch-like family member 19; KEAP1 delta C"
                     /codon_start=1
                     /product="kelch-like ECH-associated protein 1"
                     /protein_id="NP_987096.1"
                     /db_xref="CCDS:CCDS12239.1"
                     /db_xref="GeneID:9817"
                     /db_xref="HGNC:HGNC:23177"
                     /db_xref="MIM:606016"
                     /translation="MQPDPRPSGAGACCRFLPLQSQCPEGAGDAVMYASTECKAEVTP
                     SQHGNRTFSYTLEDHTKQAFGIMNELRLSQQLCDVTLQVKYQDAPAAQFMAHKVVLAS
                     SSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISMGEKCVLHVMNGAV
                     MYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCVELHQRAREYIYMHFGEVAKQE
                     EFFNLSHCQLVTLISRDDLNVRCESEVFHACINWVKYDCEQRRFYVQALLRAVRCHSL
                     TPNFLQMQLQKCEILQSDSRCKDYLVKIFEELTLHKPTQVMPCRAPKVGRLIYTAGGY
                     FRQSLSYLEAYNPSDGTWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSA
                     LDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGCIHHNSVERYEPERDEW
                     HLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITAMNTIRS
                     GAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMKHRRSALGITVHQGRI
                     YVLGGYDGHTFLDSVECYDPDTDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC
                     "
     exon            803..1488
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1694
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1871
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2565
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2536..2541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /note="hexamer: AATAAA"
     polyA_site      2565
                     /gene="KEAP1"
                     /gene_synonym="INrf2; KLHL19"
                     /note="major polyA site"
ORIGIN      
        1 cttttcgggc gtcccgaggc cgctccccaa ccgacaacca agaccccgca ggccacgcag
       61 ccctggagcc gaggcccccc gacggcggag gcgcccgcgg gtcccctaca gccaaggtcc
      121 ctgagtgcca gaggtggtgg tgttgcttat cttctggaac cccatgcagc cagatcccag
      181 gcctagcggg gctggggcct gctgccgatt cctgcccctg cagtcacagt gccctgaggg
      241 ggcaggggac gcggtgatgt acgcctccac tgagtgcaag gcggaggtga cgccctccca
      301 gcatggcaac cgcaccttca gctacaccct ggaggatcat accaagcagg cctttggcat
      361 catgaacgag ctgcggctca gccagcagct gtgtgacgtc acactgcagg tcaagtacca
      421 ggatgcaccg gccgcccagt tcatggccca caaggtggtg ctggcctcat ccagccctgt
      481 cttcaaggcc atgttcacca acgggctgcg ggagcagggc atggaggtgg tgtccattga
      541 gggtatccac cccaaggtca tggagcgcct cattgaattc gcctacacgg cctccatctc
      601 catgggcgag aagtgtgtcc tccacgtcat gaacggtgct gtcatgtacc agatcgacag
      661 cgttgtccgt gcctgcagtg acttcctggt gcagcagctg gaccccagca atgccatcgg
      721 catcgccaac ttcgctgagc agattggctg tgtggagttg caccagcgtg cccgggagta
      781 catctacatg cattttgggg aggtggccaa gcaagaggag ttcttcaacc tgtcccactg
      841 ccaactggtg accctcatca gccgggacga cctgaacgtg cgctgcgagt ccgaggtctt
      901 ccacgcctgc atcaactggg tcaagtacga ctgcgaacag cgacggttct acgtccaggc
      961 gctgctgcgg gccgtgcgct gccactcgtt gacgccgaac ttcctgcaga tgcagctgca
     1021 gaagtgcgag atcctgcagt ccgactcccg ctgcaaggac tacctggtca agatcttcga
     1081 ggagctcacc ctgcacaagc ccacgcaggt gatgccctgc cgggcgccca aggtgggccg
     1141 cctgatctac accgcgggcg gctacttccg acagtcgctc agctacctgg aggcttacaa
     1201 ccccagtgac ggcacctggc tccggttggc ggacctgcag gtgccgcgga gcggcctggc
     1261 cggctgcgtg gtgggcgggc tgttgtacgc cgtgggcggc aggaacaact cgcccgacgg
     1321 caacaccgac tccagcgccc tggactgtta caaccccatg accaatcagt ggtcgccctg
     1381 cgcccccatg agcgtgcccc gtaaccgcat cggggtgggg gtcatcgatg gccacatcta
     1441 tgccgtcggc ggctcccacg gctgcatcca ccacaacagt gtggagaggt atgagccaga
     1501 gcgggatgag tggcacttgg tggccccaat gctgacacga aggatcgggg tgggcgtggc
     1561 tgtcctcaat cgtctccttt atgccgtggg gggctttgac gggacaaacc gccttaattc
     1621 agctgagtgt tactacccag agaggaacga gtggcgaatg atcacagcaa tgaacaccat
     1681 ccgaagcggg gcaggcgtct gcgtcctgca caactgtatc tatgctgctg ggggctatga
     1741 tggtcaggac cagctgaaca gcgtggagcg ctacgatgtg gaaacagaga cgtggacttt
     1801 cgtagccccc atgaagcacc ggcgaagtgc cctggggatc actgtccacc aggggagaat
     1861 ctacgtcctt ggaggctatg atggtcacac gttcctggac agtgtggagt gttacgaccc
     1921 agatacagac acctggagcg aggtgacccg aatgacatcg ggccggagtg gggtgggcgt
     1981 ggctgtcacc atggagccct gccggaagca gattgaccag cagaactgta cctgttgagg
     2041 cacttttgtt tcttgggcaa aaatacagtc caatggggag tatcattgtt tttgtacaaa
     2101 aaccgggact aaaagaaaag acagcactgc aaataaccca tcttccggga agggaggcca
     2161 ggatgcctca gtgttaaaat gacatctcaa aagaagtcca aagcgggaat catgtgcccc
     2221 tcagcggagc cccgggagtg tccaagacag cctggctggg aaagggggtg tggaaagagc
     2281 aggcttccag gagagaggcc cccaaaccct ctggccgggt aataggcctg ggtcccactc
     2341 acccatgccg gcagctgtca ccatgtgatt tattcttgga tacctgggag ggggccaatg
     2401 ggggcctcag ggggaggccc cctctggaaa tgtggttccc agggatgggc ctgtacatag
     2461 aagccaccgg atggcacttc cccaccggat ggacagttat tttgttgata agtaaccctg
     2521 taattttcca aggaaaataa agaacagact aactagtgtc tttca
//
