LOCUS       NM_001031836            3692 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily U member
            1 (KCNU1), transcript variant 1, mRNA.
ACCESSION   NM_001031836
VERSION     NM_001031836.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Liu,R., Yan,Z., Fan,Y., Qu,R., Chen,B., Li,B., Wu,L., Wu,H., Mu,J.,
            Zhao,L., Wang,W., Dong,J., Zeng,Y., Li,Q., Wang,L., Sang,Q.,
            Zhang,Z. and Kuang,Y.
  TITLE     Bi-allelic variants in KCNU1 cause impaired acrosome reactions and
            male infertility
  JOURNAL   Hum Reprod 37 (7), 1394-1405 (2022)
   PUBMED   35551387
  REMARK    GeneRIF: Bi-allelic variants in KCNU1 cause impaired acrosome
            reactions and male infertility.
REFERENCE   3  (bases 1 to 3692)
  AUTHORS   Lv,M., Liu,C., Ma,C., Yu,H., Shao,Z., Gao,Y., Liu,Y., Wu,H.,
            Tang,D., Tan,Q., Zhang,J., Li,K., Xu,C., Geng,H., Zhang,J., Li,H.,
            Mao,X., Ge,L., Fu,F., Zhong,K., Xu,Y., Tao,F., Zhou,P., Wei,Z.,
            He,X., Zhang,F. and Cao,Y.
  TITLE     Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia
            due to acrosome hypoplasia and mitochondrial sheath malformations
  JOURNAL   Reprod Biol Endocrinol 20 (1), 5 (2022)
   PUBMED   34980136
  REMARK    GeneRIF: Homozygous mutation in SLO3 leads to severe
            asthenoteratozoospermia due to acrosome hypoplasia and
            mitochondrial sheath malformations.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3692)
  AUTHORS   Geng,Y., Ferreira,J.J., Dzikunu,V., Butler,A., Lybaert,P., Yuan,P.,
            Magleby,K.L., Salkoff,L. and Santi,C.M.
  TITLE     A genetic variant of the sperm-specific SLO3 K+ channel has altered
            pH and Ca2+ sensitivities
  JOURNAL   J Biol Chem 292 (21), 8978-8987 (2017)
   PUBMED   28377504
  REMARK    GeneRIF: results suggest that sperm-specific genes can evolve
            rapidly and that natural genetic variation may have led to a SLO3
            variant that differs from wild type in both pH and intracellular
            Ca(2+) sensitivities.
REFERENCE   5  (bases 1 to 3692)
  AUTHORS   Zeng,X.H., Yang,C., Xia,X.M., Liu,M. and Lingle,C.J.
  TITLE     SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER
            currents and is critical for normal fertility
  JOURNAL   Proc Natl Acad Sci U S A 112 (8), 2599-2604 (2015)
   PUBMED   25675513
REFERENCE   6  (bases 1 to 3692)
  AUTHORS   Brenker,C., Zhou,Y., Muller,A., Echeverry,F.A., Trotschel,C.,
            Poetsch,A., Xia,X.M., Bonigk,W., Lingle,C.J., Kaupp,U.B. and
            Strunker,T.
  TITLE     The Ca2+-activated K+ current of human sperm is mediated by Slo3
  JOURNAL   Elife 3, e01438 (2014)
   PUBMED   24670955
  REMARK    GeneRIF: The authors conclude that Slo3 represents the principal
            K(+) channel in human sperm that carries the Ca(2+)-activated
            IKSper current.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3692)
  AUTHORS   Leonetti,M.D., Yuan,P., Hsiung,Y. and Mackinnon,R.
  TITLE     Functional and structural analysis of the human SLO3 pH- and
            voltage-gated K+ channel
  JOURNAL   Proc Natl Acad Sci U S A 109 (47), 19274-19279 (2012)
   PUBMED   23129643
  REMARK    GeneRIF: These results present insights into the function of a
            protein expected to be critical for human reproduction and provide
            a framework to study the mechanism of pH gating in SLO3 channels.
REFERENCE   8  (bases 1 to 3692)
  AUTHORS   Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S.,
            Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A.,
            Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X.,
            Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T.,
            Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K.,
            DeCaprio,D., Dooley,K.T., Dorris,L. 3rd, Engels,R., Glockner,G.,
            Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B.,
            Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P.,
            Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H.,
            Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C.,
            O'Leary,S.B., O'Neill,K., Parker,S.C., Polley,A., Raymond,C.K.,
            Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R.,
            Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K.,
            Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q.,
            Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and
            Lander,E.S.
  TITLE     DNA sequence and analysis of human chromosome 8
  JOURNAL   Nature 439 (7074), 331-335 (2006)
   PUBMED   16421571
REFERENCE   9  (bases 1 to 3692)
  AUTHORS   Wei,A.D., Gutman,G.A., Aldrich,R., Chandy,K.G., Grissmer,S. and
            Wulff,H.
  TITLE     International Union of Pharmacology. LII. Nomenclature and
            molecular relationships of calcium-activated potassium channels
  JOURNAL   Pharmacol Rev 57 (4), 463-472 (2005)
   PUBMED   16382103
  REMARK    Review article
REFERENCE   10 (bases 1 to 3692)
  AUTHORS   Schreiber,M., Wei,A., Yuan,A., Gaut,J., Saito,M. and Salkoff,L.
  TITLE     Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes
  JOURNAL   J Biol Chem 273 (6), 3509-3516 (1998)
   PUBMED   9452476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC401954.1, BC028701.2,
            AC124076.5 and AC138136.2.
            
            On Nov 22, 2018 this sequence version replaced NM_001031836.2.
            
            Summary: This gene encodes a member of the potassium channel family
            of proteins. The encoded voltage-gated ion channel allows the
            outward flow of potassium ions during plasma membrane
            hyperpolarization in sperm. Opening of this channel may be
            regulated by calcium ion levels. Homozygous knockout mice that lack
            the related mouse gene exhibit male sterility. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Dec
            2015].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302391.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399881.8/ ENSP00000382770.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DC401954.1         51-59
            10-610              BC028701.2         1-601
            611-611             AC124076.5         53035-53035         c
            612-807             BC028701.2         603-798
            808-808             AC124076.5         45164-45164         c
            809-2252            BC028701.2         800-2243
            2253-2253           AC138136.2         78510-78510
            2254-2338           BC028701.2         2245-2329
            2339-2339           AC138136.2         78596-78596
            2340-2773           BC028701.2         2331-2764
            2774-3692           BC028701.2         2827-3745
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     gene            1..3692
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /note="potassium calcium-activated channel subfamily U
                     member 1"
                     /db_xref="GeneID:157855"
                     /db_xref="HGNC:HGNC:18867"
                     /db_xref="MIM:615215"
     exon            1..232
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..3487
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /note="Slowpoke homolog 3; potassium channel, subfamily U,
                     member 1; Calcium-activated potassium channel subunit
                     alpha-3; Calcium-activated potassium channel, subfamily M
                     subunit alpha-3"
                     /codon_start=1
                     /product="potassium channel subfamily U member 1"
                     /protein_id="NP_001027006.2"
                     /db_xref="CCDS:CCDS55220.1"
                     /db_xref="GeneID:157855"
                     /db_xref="HGNC:HGNC:18867"
                     /db_xref="MIM:615215"
                     /translation="MFQTKLRNETWEDLPKMSCTTEIQAAFILSSFVTFFSGLIILLI
                     FRLIWRSVKKWQIIKGTGIILELFTSGTIARSHVRSLHFQGQFRDHIEMLLSAQTFVG
                     QVLVILVFVLSIGSLIIYFINSADPVGSCSSYEDKTIPIDLVFNAFFSFYFGLRFMAA
                     DDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPQILQILRAIKT
                     SNSVKFSKLLSIILSTWFTAAGFIHLVENSGDPWLKGRNSQNISYFESIYLVMATTST
                     VGFGDVVAKTSLGRTFIMFFTLGSLILFANYIPEMVELFANKRKYTSSYEALKGKKFI
                     VVCGNITVDSVTAFLRNFLRDKSGEINTEIVFLGETPPSLELETIFKCYLAYTTFISG
                     SAMKWEDLRRVAVESAEACLIIANPLCSDSHAEDISNIMRVLSIKNYDSTTRIIIQIL
                     QSHNKVYLPKIPSWNWDTGDNIICFAELKLGFIAQGCLVPGLCTFLTSLFVEQNKKVM
                     PKQTWKKHFLNSMKNKILTQRLSDDFAGMSFPEVARLCFLKMHLLLIAIEYKSLFTDG
                     FCGLILNPPPQVRIRKNTLGFFIAETPKDVRRALFYCSVCHDDVFIPELITNCGCKSR
                     SRQHITVPSVKRMKKCLKGISSRISGQDSPPRVSASTSSISNFTTRTLQHDVEQDSDQ
                     LDSSGMFHWCKPTSLDKVTLKRTGKSKYKFRNHIVACVFGDAHSAPMGLRNFVMPLRA
                     SNYTRKELKDIVFIGSLDYLQREWRFLWNFPQIYILPGCALYSGDLHAANIEQCSMCA
                     VLSPPPQPSSNQTLVDTEAIMATLTIGSLQIDSSSDPSPSVSEETPGYTNGHNEKSNC
                     RKVPILTELKNPSNIHFIEQLGGLEGSLQETNLHLSTAFSTGTVFSGSFLDSLLATAF
                     YNYHVLELLQMLVTGGVSSQLEQHLDKDKVYGVADSCTSLLSGRNRCKLGLLSLHETI
                     LSDVNPRNTFGQLFCGSLDLFGILCVGLYRIIDEEELNPENKRFVITRPANEFKLLPS
                     DLVFCAIPFSTACYKRNEEFSLQKSYEIVNKASQTTETHSDTNCPPTIDSVTETLYSP
                     VYSYQPRTNSLSFPKQIAWNQSRTNSIISSQIPLGDNAKENERKTSDEVYDEDPFAYS
                     EPL"
     exon            233..352
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            353..414
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            415..505
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            506..617
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            618..693
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            694..769
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            770..940
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1032
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1143
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1249
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1332
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1402
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1555
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1668
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1740
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1830
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1928
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2046
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2143
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2368
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2558
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2633
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2773
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2774..2968
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3081
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3692
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3676..3681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /note="hexamer: AAGAAA"
     polyA_site      3692
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /note="major polyA site"
ORIGIN      
        1 aaatgacctg gcaattccgt ctactgatgt ctcgaacatg tttcagacta agctacgaaa
       61 tgaaacttgg gaagacttgc caaaaatgtc ctgcacaact gagatccaag cagcattcat
      121 tctctcttcc tttgtgacct tcttcagtgg actcatcatc ctgttgatct tcaggctgat
      181 ctggagatct gttaaaaaat ggcaaatcat caagggaaca ggaattatct tggaactgtt
      241 cacatcaggt accatcgcta ggagccatgt aagaagcctc cacttccagg gacaatttcg
      301 tgatcatata gaaatgttgc tttcagccca gacctttgtg gggcaagtgt tggtgatcct
      361 tgtctttgta ctaagcattg ggtctcttat aatctatttc atcaattctg ctgaccctgt
      421 tggaagctgt tcatcatatg aagacaaaac cattcctatt gatttggttt tcaatgcttt
      481 ctttagtttc tattttggat tgaggtttat ggcagctgat gacaagatca agttctggct
      541 ggagatgaat tcaatcgtag acatctttac catcccacca acctttattt cttattattt
      601 gaagagcaat tggctaggtt taaggttcct aagagccttg cgcctgctag aactccctca
      661 aatcttgcaa attctacgag ccatcaagac cagtaactca gtgaagtttt ccaaactgct
      721 gtcaataatt ctcagtacct ggttcacagc tgcgggattc attcacctgg tggaaaattc
      781 tggtgatccc tggctcaaag gtagaaattc acagaatata tcatattttg agtcaattta
      841 cctggtcatg gcaacaacgt caaccgttgg atttggagat gtggtagcca agacatcctt
      901 aggacggacc ttcatcatgt tcttcacact ggggagtttg atattatttg cgaactatat
      961 acctgaaatg gtggaactgt ttgctaacaa gaggaaatac accagttcct atgaagcact
     1021 caaaggaaag aagtttattg tggtctgtgg aaacatcact gtggacagtg tgaccgcttt
     1081 cctgaggaat ttcctccgcg acaagtcagg agagatcaac actgaaattg ttttcctggg
     1141 agaaacccct ccttctttgg aacttgaaac catatttaaa tgctacttgg cctacacaac
     1201 gttcatttct ggatctgcaa tgaagtggga ggatctgagg cgagttgcgg tggaatctgc
     1261 agaggcatgc ctgattatag ccaatccttt gtgcagtgat tcccatgctg aagatatttc
     1321 caacattatg agggtgctct ctatcaagaa ctatgattct accaccagaa tcatcataca
     1381 gatactgcaa tcccataaca aggtttatct gccaaagatt cccagctgga actgggacac
     1441 cggagacaac atcatctgct ttgctgaatt aaaacttgga tttatcgccc aaggctgttt
     1501 ggtgccaggc ttgtgtacct tcctaacatc tctatttgtg gagcaaaaca aaaaggttat
     1561 gcctaaacag acctggaaga aacacttctt gaatagcatg aaaaacaaaa ttctgaccca
     1621 acgtctctct gatgactttg ctggaatgag ctttcctgaa gttgcccggc tctgctttct
     1681 gaagatgcac ctcctgttga tagccatcga atacaagtcc ctctttacgg atggtttctg
     1741 tggtctgata ctaaatccac ctccacaagt gaggatacgt aagaacacat tagggttctt
     1801 tattgctgaa actccaaagg acgtcagaag agccttgttt tactgttcag tctgtcatga
     1861 tgatgtgttc attcctgagc taattacaaa ctgtggctgc aaaagcagaa gccggcagca
     1921 catcacagtg ccatcggtaa agagaatgaa aaaatgtctg aagggaatct cctctcgtat
     1981 atcagggcag gattctccgc caagggtatc tgcaagcact tcgagcatat caaacttcac
     2041 caccaggact cttcaacatg atgtagaaca agattctgac cagcttgata gcagtgggat
     2101 gtttcactgg tgcaaaccaa cctctttgga caaggtgact ctgaaacgaa ctggcaagtc
     2161 aaagtataag tttcggaacc atattgtagc atgtgtattt ggagatgccc actcagcccc
     2221 gatggggctt cggaactttg taatgccctt gagagccagc aactatacca ggaaggagct
     2281 gaaggacata gtgttcattg ggtctctgga ctatctacag agagaatggc gatttctctg
     2341 gaattttccc cagatataca ttctgcctgg atgtgcactt tattctggag acctccatgc
     2401 ggccaacata gagcaatgct ccatgtgtgc tgtcttgtcc cccccacccc agccatcaag
     2461 caaccagact ttggtagaca cagaagccat catggcaacc ctcaccatcg gatccttgca
     2521 aattgactcc tcctctgacc cgtcaccctc agtgtcagag gagactccag gttacacaaa
     2581 tggacataat gagaaatcaa actgccgaaa agtccctatc cttactgaac tgaaaaatcc
     2641 ttccaacatt cactttattg aacagcttgg tggactggaa gggtccctcc aagaaacaaa
     2701 tctgcatctc agcactgcct tttctacggg cactgttttt tccggcagct tcttggattc
     2761 tctgctggcc acggccttct acaattatca tgtcctggaa ttgcttcaga tgctggtgac
     2821 aggaggagta agttctcagc tggaacaaca tttagataag gataaagtct atggtgtggc
     2881 agatagctgc acgtcgctct tgtctggaag aaaccggtgt aagctggggc ttctgtcctt
     2941 acacgaaacc attttatcag acgttaatcc aagaaacacc tttggacaac tgttctgtgg
     3001 ctcattagat ctttttggaa tcctgtgtgt tggcttatac cgaataattg atgaagagga
     3061 gctcaaccca gaaaacaaaa ggtttgtgat cacccggcca gccaatgagt tcaagctgct
     3121 gccttcagat cttgtgtttt gtgccatacc cttcagcact gcttgttata aaaggaatga
     3181 agagttctca ttgcaaaagt catatgaaat tgtaaataaa gcatcacaga caacagagac
     3241 acattcagac acaaattgtc ctcccaccat tgattcagtt actgagacat tgtattcacc
     3301 agtctattct taccagccga gaactaactc cctctctttt cctaagcaaa tagcatggaa
     3361 tcagagtaga acaaacagta ttatatcatc tcagatacct ttaggtgaca atgcaaaaga
     3421 aaatgaaagg aaaacttcag atgaggttta tgatgaggat ccctttgcat attcagagcc
     3481 actatagacc tgcccatatt cttcacgtgc tcttaacttg ctgcttacaa atcatctcct
     3541 gagatgctaa ctttgaacaa agaaaataag aatggaagca tgccattttt ctgcccattg
     3601 cttagtggtt catgaaggcc acactgtttt gggtgagaca aaagtctaat gccactggat
     3661 cttgtgtgat aaataaagaa atatatgatc aa
//
