LOCUS       NP_001027006            1149 aa            linear   PRI 27-APR-2025
DEFINITION  potassium channel subfamily U member 1 [Homo sapiens].
ACCESSION   NP_001027006
VERSION     NP_001027006.2
DBSOURCE    REFSEQ: accession NM_001031836.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1149)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   2  (residues 1 to 1149)
  AUTHORS   Liu,R., Yan,Z., Fan,Y., Qu,R., Chen,B., Li,B., Wu,L., Wu,H., Mu,J.,
            Zhao,L., Wang,W., Dong,J., Zeng,Y., Li,Q., Wang,L., Sang,Q.,
            Zhang,Z. and Kuang,Y.
  TITLE     Bi-allelic variants in KCNU1 cause impaired acrosome reactions and
            male infertility
  JOURNAL   Hum Reprod 37 (7), 1394-1405 (2022)
   PUBMED   35551387
  REMARK    GeneRIF: Bi-allelic variants in KCNU1 cause impaired acrosome
            reactions and male infertility.
REFERENCE   3  (residues 1 to 1149)
  AUTHORS   Lv,M., Liu,C., Ma,C., Yu,H., Shao,Z., Gao,Y., Liu,Y., Wu,H.,
            Tang,D., Tan,Q., Zhang,J., Li,K., Xu,C., Geng,H., Zhang,J., Li,H.,
            Mao,X., Ge,L., Fu,F., Zhong,K., Xu,Y., Tao,F., Zhou,P., Wei,Z.,
            He,X., Zhang,F. and Cao,Y.
  TITLE     Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia
            due to acrosome hypoplasia and mitochondrial sheath malformations
  JOURNAL   Reprod Biol Endocrinol 20 (1), 5 (2022)
   PUBMED   34980136
  REMARK    GeneRIF: Homozygous mutation in SLO3 leads to severe
            asthenoteratozoospermia due to acrosome hypoplasia and
            mitochondrial sheath malformations.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 1149)
  AUTHORS   Geng,Y., Ferreira,J.J., Dzikunu,V., Butler,A., Lybaert,P., Yuan,P.,
            Magleby,K.L., Salkoff,L. and Santi,C.M.
  TITLE     A genetic variant of the sperm-specific SLO3 K+ channel has altered
            pH and Ca2+ sensitivities
  JOURNAL   J Biol Chem 292 (21), 8978-8987 (2017)
   PUBMED   28377504
  REMARK    GeneRIF: results suggest that sperm-specific genes can evolve
            rapidly and that natural genetic variation may have led to a SLO3
            variant that differs from wild type in both pH and intracellular
            Ca(2+) sensitivities.
REFERENCE   5  (residues 1 to 1149)
  AUTHORS   Zeng,X.H., Yang,C., Xia,X.M., Liu,M. and Lingle,C.J.
  TITLE     SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER
            currents and is critical for normal fertility
  JOURNAL   Proc Natl Acad Sci U S A 112 (8), 2599-2604 (2015)
   PUBMED   25675513
REFERENCE   6  (residues 1 to 1149)
  AUTHORS   Brenker,C., Zhou,Y., Muller,A., Echeverry,F.A., Trotschel,C.,
            Poetsch,A., Xia,X.M., Bonigk,W., Lingle,C.J., Kaupp,U.B. and
            Strunker,T.
  TITLE     The Ca2+-activated K+ current of human sperm is mediated by Slo3
  JOURNAL   Elife 3, e01438 (2014)
   PUBMED   24670955
  REMARK    GeneRIF: The authors conclude that Slo3 represents the principal
            K(+) channel in human sperm that carries the Ca(2+)-activated
            IKSper current.
            Publication Status: Online-Only
REFERENCE   7  (residues 1 to 1149)
  AUTHORS   Leonetti,M.D., Yuan,P., Hsiung,Y. and Mackinnon,R.
  TITLE     Functional and structural analysis of the human SLO3 pH- and
            voltage-gated K+ channel
  JOURNAL   Proc Natl Acad Sci U S A 109 (47), 19274-19279 (2012)
   PUBMED   23129643
  REMARK    GeneRIF: These results present insights into the function of a
            protein expected to be critical for human reproduction and provide
            a framework to study the mechanism of pH gating in SLO3 channels.
REFERENCE   8  (residues 1 to 1149)
  AUTHORS   Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S.,
            Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A.,
            Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X.,
            Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T.,
            Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K.,
            DeCaprio,D., Dooley,K.T., Dorris,L. 3rd, Engels,R., Glockner,G.,
            Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B.,
            Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P.,
            Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H.,
            Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C.,
            O'Leary,S.B., O'Neill,K., Parker,S.C., Polley,A., Raymond,C.K.,
            Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R.,
            Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K.,
            Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q.,
            Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and
            Lander,E.S.
  TITLE     DNA sequence and analysis of human chromosome 8
  JOURNAL   Nature 439 (7074), 331-335 (2006)
   PUBMED   16421571
REFERENCE   9  (residues 1 to 1149)
  AUTHORS   Wei,A.D., Gutman,G.A., Aldrich,R., Chandy,K.G., Grissmer,S. and
            Wulff,H.
  TITLE     International Union of Pharmacology. LII. Nomenclature and
            molecular relationships of calcium-activated potassium channels
  JOURNAL   Pharmacol Rev 57 (4), 463-472 (2005)
   PUBMED   16382103
  REMARK    Review article
REFERENCE   10 (residues 1 to 1149)
  AUTHORS   Schreiber,M., Wei,A., Yuan,A., Gaut,J., Saito,M. and Salkoff,L.
  TITLE     Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes
  JOURNAL   J Biol Chem 273 (6), 3509-3516 (1998)
   PUBMED   9452476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC401954.1, BC028701.2,
            AC124076.5 and AC138136.2.
            
            On Sep 11, 2007 this sequence version replaced NP_001027006.1.
            
            Summary: This gene encodes a member of the potassium channel family
            of proteins. The encoded voltage-gated ion channel allows the
            outward flow of potassium ions during plasma membrane
            hyperpolarization in sperm. Opening of this channel may be
            regulated by calcium ion levels. Homozygous knockout mice that lack
            the related mouse gene exhibit male sterility. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Dec
            2015].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302391.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399881.8/ ENSP00000382770.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     Protein         1..1149
                     /product="potassium channel subfamily U member 1"
                     /note="Slowpoke homolog 3; potassium channel, subfamily U,
                     member 1; Calcium-activated potassium channel subunit
                     alpha-3; Calcium-activated potassium channel, subfamily M
                     subunit alpha-3"
                     /calculated_mol_wt=129413
     Site            25..45
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Site            102..122
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Region          103..323
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Site            139..159
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Site            164..184
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Site            189..209
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Site            227..247
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Region          276..279
                     /region_name="Selectivity for potassium"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Site            292..312
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Region          470..557
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     Region          828..854
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     Region          1118..1149
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (A8MYU2.2)"
     CDS             1..1149
                     /gene="KCNU1"
                     /gene_synonym="KCa5; KCa5.1; Kcnma3; KCNMC1; Slo3; SPGF79"
                     /coded_by="NM_001031836.3:38..3487"
                     /db_xref="CCDS:CCDS55220.1"
                     /db_xref="GeneID:157855"
                     /db_xref="HGNC:HGNC:18867"
                     /db_xref="MIM:615215"
ORIGIN      
        1 mfqtklrnet wedlpkmsct teiqaafils sfvtffsgli illifrliwr svkkwqiikg
       61 tgiilelfts gtiarshvrs lhfqgqfrdh iemllsaqtf vgqvlvilvf vlsigsliiy
      121 finsadpvgs cssyedktip idlvfnaffs fyfglrfmaa ddkikfwlem nsivdiftip
      181 ptfisyylks nwlglrflra lrllelpqil qilraiktsn svkfskllsi ilstwftaag
      241 fihlvensgd pwlkgrnsqn isyfesiylv mattstvgfg dvvaktslgr tfimfftlgs
      301 lilfanyipe mvelfankrk ytssyealkg kkfivvcgni tvdsvtaflr nflrdksgei
      361 nteivflget ppsleletif kcylayttfi sgsamkwedl rrvavesaea cliianplcs
      421 dshaedisni mrvlsiknyd sttriiiqil qshnkvylpk ipswnwdtgd niicfaelkl
      481 gfiaqgclvp glctfltslf veqnkkvmpk qtwkkhflns mknkiltqrl sddfagmsfp
      541 evarlcflkm hllliaieyk slftdgfcgl ilnpppqvri rkntlgffia etpkdvrral
      601 fycsvchddv fipelitncg cksrsrqhit vpsvkrmkkc lkgissrisg qdspprvsas
      661 tssisnfttr tlqhdveqds dqldssgmfh wckptsldkv tlkrtgksky kfrnhivacv
      721 fgdahsapmg lrnfvmplra snytrkelkd ivfigsldyl qrewrflwnf pqiyilpgca
      781 lysgdlhaan ieqcsmcavl spppqpssnq tlvdteaima tltigslqid sssdpspsvs
      841 eetpgytngh neksncrkvp iltelknpsn ihfieqlggl egslqetnlh lstafstgtv
      901 fsgsfldsll atafynyhvl ellqmlvtgg vssqleqhld kdkvygvads ctsllsgrnr
      961 cklgllslhe tilsdvnprn tfgqlfcgsl dlfgilcvgl yriideeeln penkrfvitr
     1021 panefkllps dlvfcaipfs tacykrneef slqksyeivn kasqtteths dtncpptids
     1081 vtetlyspvy syqprtnsls fpkqiawnqs rtnsiissqi plgdnakene rktsdevyde
     1141 dpfaysepl
//
