LOCUS       NM_198503               5984 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens potassium sodium-activated channel subfamily T member
            2 (KCNT2), transcript variant 1, mRNA.
ACCESSION   NM_198503 XM_291574
VERSION     NM_198503.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Cioclu,M.C., Mosca,I., Ambrosino,P., Puzo,D., Bayat,A.,
            Wortmann,S.B., Koch,J., Strehlow,V., Shirai,K., Matsumoto,N.,
            Sanders,S.J., Michaud,V., Legendre,M., Riva,A., Striano,P.,
            Muhle,H., Pendziwiat,M., Lesca,G., Mangano,G.D., Nardello,R.,
            Lemke,J.R., Moller,R.S., Soldovieri,M.V., Rubboli,G. and
            Taglialatela,M.
  CONSRTM   KCNT2-study group
  TITLE     KCNT2-Related Disorders: Phenotypes, Functional, and
            Pharmacological Properties
  JOURNAL   Ann Neurol 94 (2), 332-349 (2023)
   PUBMED   37062836
  REMARK    GeneRIF: KCNT2-Related Disorders: Phenotypes, Functional, and
            Pharmacological Properties.
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Jackson,A., Banka,S., Stewart,H., Robinson,H., Lovell,S. and
            Clayton-Smith,J.
  CONSRTM   Genomics England Research Consortium
  TITLE     Recurrent KCNT2 missense variants affecting p.Arg190 result in a
            recognizable phenotype
  JOURNAL   Am J Med Genet A 185 (10), 3083-3091 (2021)
   PUBMED   34061450
  REMARK    GeneRIF: Recurrent KCNT2 missense variants affecting p.Arg190
            result in a recognizable phenotype.
REFERENCE   3  (bases 1 to 5984)
  AUTHORS   Alagoz,M., Kherad,N., Bozkurt,S. and Yuksel,A.
  TITLE     New mutations in KCNT2 gene causing early infantile epileptic
            encephalopathy type 57: Case study and literature review
  JOURNAL   Acta Biochim Pol 67 (3), 431-434 (2020)
   PUBMED   32931186
  REMARK    GeneRIF: New mutations in KCNT2 gene causing early infantile
            epileptic encephalopathy type 57: Case study and literature review.
            Review article
REFERENCE   4  (bases 1 to 5984)
  AUTHORS   Liu,B., Ye,B., Zhu,X., Yang,L., Li,H., Liu,N., Zhu,P., Lu,T.,
            He,L., Tian,Y. and Fan,Z.
  TITLE     An inducible circular RNA circKcnt2 inhibits ILC3 activation to
            facilitate colitis resolution
  JOURNAL   Nat Commun 11 (1), 4076 (2020)
   PUBMED   32796851
  REMARK    GeneRIF: An inducible circular RNA circKcnt2 inhibits ILC3
            activation to facilitate colitis resolution.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5984)
  AUTHORS   Amador-Munoz,D., Gutierrez,A.M., Payan-Gomez,C. and Matheus,L.M.
  TITLE     In silico and in vitro analysis of cation-activated potassium
            channels in human corneal endothelial cells
  JOURNAL   Exp Eye Res 197, 108114 (2020)
   PUBMED   32561484
  REMARK    GeneRIF: In silico and in vitro analysis of cation-activated
            potassium channels in human corneal endothelial cells.
            Erratum:[Exp Eye Res. 2021 Oct;211:108757. doi:
            10.1016/j.exer.2021.108757.. PMID: 34507812]
REFERENCE   6  (bases 1 to 5984)
  AUTHORS   Tejada,M.A., Stople,K., Hammami Bomholtz,S., Meinild,A.K.,
            Poulsen,A.N. and Klaerke,D.A.
  TITLE     Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2)
            K+ channels
  JOURNAL   PLoS One 9 (10), e110833 (2014)
   PUBMED   25347289
  REMARK    GeneRIF: Slick channels, in contrast to the similar Slack channels,
            are the only high-conductance K+ channels strongly sensitive to
            small changes in cell volume.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5984)
  AUTHORS   Garg,P., Gardner,A., Garg,V. and Sanguinetti,M.C.
  TITLE     Structural basis of ion permeation gating in Slo2.1 K+ channels
  JOURNAL   J Gen Physiol 142 (5), 523-542 (2013)
   PUBMED   24166878
  REMARK    GeneRIF: Together these findings suggest that (1) the selectivity
            filter and not the bundle crossing gates ion permeation and (2)
            dynamic coupling between the pore helix and the S5 and S6 segments
            mediates Slo2.1 channel activation.
REFERENCE   8  (bases 1 to 5984)
  AUTHORS   Santi,C.M., Ferreira,G., Yang,B., Gazula,V.R., Butler,A., Wei,A.,
            Kaczmarek,L.K. and Salkoff,L.
  TITLE     Opposite regulation of Slick and Slack K+ channels by
            neuromodulators
  JOURNAL   J Neurosci 26 (19), 5059-5068 (2006)
   PUBMED   16687497
REFERENCE   9  (bases 1 to 5984)
  AUTHORS   Wei,A.D., Gutman,G.A., Aldrich,R., Chandy,K.G., Grissmer,S. and
            Wulff,H.
  TITLE     International Union of Pharmacology. LII. Nomenclature and
            molecular relationships of calcium-activated potassium channels
  JOURNAL   Pharmacol Rev 57 (4), 463-472 (2005)
   PUBMED   16382103
  REMARK    Review article
REFERENCE   10 (bases 1 to 5984)
  AUTHORS   Bhattacharjee,A., Joiner,W.J., Wu,M., Yang,Y., Sigworth,F.J. and
            Kaczmarek,L.K.
  TITLE     Slick (Slo2.1), a rapidly-gating sodium-activated potassium channel
            inhibited by ATP
  JOURNAL   J Neurosci 23 (37), 11681-11691 (2003)
   PUBMED   14684870
  REMARK    GeneRIF: Cloning and regulation of slick channel activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB071968.1, AY359444.1 and AL139137.15.
            
            On Jun 2, 2019 this sequence version replaced NM_198503.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY359444.1, EU251076.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000294725.14/ ENSP00000294725.8
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                DB071968.1         466-536
            72-3139             AY359444.1         1-3068
            3140-3140           AL139137.15        109249-109249       c
            3141-3624           AY359444.1         3070-3553
            3625-5984           AL139137.15        76632-78991         c
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.3"
     gene            1..5984
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /note="potassium sodium-activated channel subfamily T
                     member 2"
                     /db_xref="GeneID:343450"
                     /db_xref="HGNC:HGNC:18866"
                     /db_xref="MIM:610044"
     exon            1..226
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..3539
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium-and chloride-activated ATP-sensitive potassium
                     channel (SLICK); sequence like an intermediate conductance
                     potassium channel subunit; sodium and chloride-activated
                     ATP-sensitive potassium channel Slo2.1; potassium channel,
                     subfamily T, member 2"
                     /codon_start=1
                     /product="potassium channel subfamily T member 2 isoform
                     1"
                     /protein_id="NP_940905.2"
                     /db_xref="CCDS:CCDS1384.1"
                     /db_xref="GeneID:343450"
                     /db_xref="HGNC:HGNC:18866"
                     /db_xref="MIM:610044"
                     /translation="MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTF
                     KERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLENPSQGNEWSHIFWVNRSL
                     PLWGLQVSVALISLFETILLGYLSYKGNIWEQILRIPFILEIINAVPFIISIFWPSLR
                     NLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQ
                     HLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQ
                     LAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVI
                     LCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDDAEACFILSSRCEVDR
                     TSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCI
                     CPATSTLITLLVHTSRGQEGQQSPEQWQKMYGRCSGNEVYHIVLEESTFFAEYEGKSF
                     TYASFHAHKKFGVCLIGVRREDNKNILLNPGPRYIMNSTDICFYINITKEENSAFKNQ
                     DQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRSASGPTLSLPTEGSKEI
                     RRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEEMSSNLEYAKGYPPYSPYIGSSP
                     TFCHLLHEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLR
                     AYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANM
                     VVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCY
                     SLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLL
                     GLDTTPGSGFLCSMKITADDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQ
                     ISISVEEWEDTKDSKEQGHHRSNHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSG
                     KTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPS
                     PDTRIELNDVVYLIRPDPLAYLPNSEPSRRNSICNVTGQDSREETQL"
     exon            227..306
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            307..406
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..455
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..515
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            516..590
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            591..674
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            675..769
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            770..950
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1115
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1252
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1316
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1425
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1534
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1684
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1914
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2128
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2234
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2407
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2479
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2480..2614
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2615..2726
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2727..2828
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..2912
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3041
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3342
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3343..3427
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3428..5984
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5959..5964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /note="hexamer: AATAAA"
     polyA_site      5984
                     /gene="KCNT2"
                     /gene_synonym="DEE57; EIEE57; KCa4.2; KNa1.2; SLICK;
                     SLO2.1"
                     /note="major polyA site"
ORIGIN      
        1 cttccccttc tcccatccct ctctcggcca cagcgtcttg ttagtcctct ccctgtactc
       61 cgcaatattt tctttctttc tccctcctct cctccatttg ttgtttgatg tttcccactc
      121 tttgaggaag gatggttgat ttggagagcg aagtgccccc tctgcctccc aggtacaggt
      181 ttcgagattt gctgctaggg gaccaaggat ggcaaaacga cgacagggta caagttgaat
      241 tctatatgaa tgaaaataca tttaaagaaa gactaaaatt atttttcata aaaaaccaga
      301 gatcaagtct aaggatacgc ctgttcaatt tttctctcaa attactaagc tgcttattat
      361 acataatccg agtactacta gaaaaccctt cacaaggaaa tgaatggtct catatctttt
      421 gggtgaacag aagtctacct ttgtggggct tacaggtttc agtggcattg ataagtctgt
      481 ttgaaacaat attacttggt tatcttagtt ataagggaaa catctgggaa cagattttac
      541 gaataccctt catcttggaa ataattaatg cagttccctt cattatctca atattctggc
      601 cttccttaag gaatctattt gtcccagtct ttctgaactg ttggcttgcc aaacatgcct
      661 tggaaaatat gattaatgat ctacacagag ccattcagcg tacacagtct gcaatgttta
      721 atcaagtttt gattttaata tctacattac tatgccttat cttcacctgc atttgtggga
      781 tccaacatct ggaacgaata ggaaagaagc tgaatctctt tgactccctt tatttctgca
      841 ttgtgacgtt ttctactgtg ggcttcgggg atgtcactcc tgaaacatgg tcctccaagc
      901 tttttgtagt tgctatgatt tgtgttgctc ttgtggttct acccatacag tttgaacagc
      961 tggcttattt gtggatggag agacaaaagt caggaggaaa ctatagtcga catagagctc
     1021 aaactgaaaa gcatgtcgtc ctgtgtgtca gctcactgaa gattgattta cttatggatt
     1081 ttttaaatga attctatgct catcctaggc tccaggatta ttatgtggtg attttgtgtc
     1141 ctactgaaat ggatgtacag gttcgaaggg tactgcagat tccaatgtgg tcccaacgag
     1201 ttatctacct tcaaggttca gcccttaaag atcaagacct attgagagca aagatggatg
     1261 acgctgaggc ctgttttatt ctcagtagcc gttgtgaagt ggataggaca tcatctgatc
     1321 accaaacaat tttgagagca tgggctgtga aagattttgc tccaaattgt cctttgtatg
     1381 tccagatatt aaagcctgaa aataaatttc acatcaaatt tgctgatcat gttgtttgtg
     1441 aagaagagtt taaatacgcc atgttagctt taaactgtat atgcccagca acatctacac
     1501 ttattacact actggttcat acctctagag ggcaagaagg ccagcaatcg ccagaacaat
     1561 ggcagaagat gtacggtaga tgctccggga atgaagtcta ccacattgtt ttggaagaaa
     1621 gtacattttt tgctgaatat gaaggaaaga gttttacata tgcctctttc catgcacaca
     1681 aaaagtttgg cgtctgcttg attggtgtta ggagggagga taataaaaac attttgctga
     1741 atccaggtcc tcgatacatt atgaattcta cagacatatg cttttatatt aatattacca
     1801 aagaagagaa ttcagcattt aaaaaccaag accagcagag aaaaagcaat gtgtccaggt
     1861 cgttttatca tggaccttcc agattacctg tacatagcat aattgccagc atgggtactg
     1921 tggctataga cttgcaagat acaagctgta gatcagcaag tggccctacc ctgtctcttc
     1981 ctacagaggg aagcaaagaa ataagaagac ctagcattgc tcctgtttta gaggttgcag
     2041 atacatcatc gattcaaaca tgtgatcttc taagtgacca atcagaagat gaaactacac
     2101 cagatgaaga aatgtcttca aacttagagt atgctaaagg ttacccacct tattctccat
     2161 atataggaag ttcacccact ttttgtcatc tccttcatga aaaagtacca ttttgctgct
     2221 taagattaga caagagttgc caacataact actatgagga tgcaaaagcc tatggattca
     2281 aaaataaact aattatagtt gcagctgaaa cagctggaaa tggattatat aactttattg
     2341 ttcctctcag ggcatattat agaccaaaga aagaacttaa tcccatagta ctgctattgg
     2401 ataacccgcc agatatgcat tttctggatg caatctgttg gtttccaatg gtttactaca
     2461 tggtgggctc tattgacaac ctagatgact tactcaggtg tggagtgact tttgctgcta
     2521 atatggtggt tgtggataaa gagagcacca tgagtgccga ggaagactac atggcagatg
     2581 ccaaaaccat tgtgaacgtg cagacactct tcaggttgtt ttccagtctc agtattatca
     2641 cagagctaac tcaccccgcc aacatgagat tcatgcaatt cagagccaaa gactgttact
     2701 ctcttgctct ttcaaaactg gaaaagaaag aacgggagag aggctctaac ttggccttta
     2761 tgtttcgact gccttttgct gctgggaggg tgtttagcat cagtatgttg gacactctgc
     2821 tgtatcagtc atttgtgaag gattatatga tttctatcac gagacttctg ttgggactgg
     2881 acactacacc aggatctggg tttctttgtt ctatgaaaat cactgcagat gacttatgga
     2941 tcagaactta tgccagactt tatcagaagt tgtgttcttc tactggagat gttcccattg
     3001 gaatctacag gactgagtct cagaaactta ctacatctga gtctcaaata tctatcagtg
     3061 tagaagagtg ggaagacacc aaagactcca aagaacaagg gcaccaccgc agcaaccacc
     3121 gcaactcaac atccagtgat cagtcggacc atcccttgct gcggagaaaa agcatgcagt
     3181 gggcccgaag actgagcaga aaaggcccaa aacactctgg taaaacagct gaaaaaataa
     3241 cccagcagcg actgaacctc tacaggaggt cagaaagaca agagcttgct gaacttgtga
     3301 aaaatagaat gaaacacttg ggtctttcta cagtgggata tgatgaaatg aatgatcatc
     3361 aaagtaccct ctcctacatc ctgattaacc catctccaga taccagaata gagctgaatg
     3421 atgttgtata cttaattcga ccagatccac tggcctacct tccaaacagt gagcccagtc
     3481 gaagaaacag catctgcaat gtcactggtc aagattctcg ggaggaaact caactttgat
     3541 aaaaataaaa tgagaaactt ttttcctaca aagaccttgc ttgaaaccac aaaagttttg
     3601 ctggcacgaa agaaactaga tggaaatata tgtaattctc tcatatttaa aaacgtaatc
     3661 tcttctctta gaagtataga tcattttgaa acttaatgta ctacttactg gtactctccc
     3721 tattaatatt tgaaggacct caatggaata aatttgaaaa gctaaattaa aatacaaaaa
     3781 tttaaatctg acatttaatt gttttataat aatccaaact ctatgaaagc aattttaaaa
     3841 attattaagg ttttatgaag ttgacaaaat ctaactatat ttggtgcatc acaatggaca
     3901 cagaatgctg ctgctcctct taaaaattaa atgtgtcata ttatattctt taaacttact
     3961 gttttacaaa attgagctca tcgtaaatgt ctagtcttct cacatagaga ttaaccaaca
     4021 aacttgtgtg gctgactttt gtgtaagaat catagtttgc tttagaatac aaatctttaa
     4081 gtcattttaa cttttttttc tgccttacga tataaaaata tttatcttag aatttgagat
     4141 gttcatagca tgttttatta cattgaagaa actaaaacat aaatgaaaag aaacactagg
     4201 ttcctgcact ttttggtaac tttatgtcta gcaaatattt tatgccaaga aaagcatact
     4261 ataaagcaaa tatctattat tctcctaaac gaatgcctag catagagaaa atacttaata
     4321 cacatttgtt gacttaaatt taattcaagg attgaaaaat taactggata tcttgaaata
     4381 tacagtaatg attgtcctta gactcttgaa ctttaccatc tttcctattc atatatctat
     4441 atagtaaatt tcactagaaa aattctttta aaattgacag aagataattt atacctttta
     4501 tggactctga agacacttca aaacattaaa agtccttatg tctttggtaa tgaaacaata
     4561 acactcaatg aaggatgtat taaaattttt gacttaattt tgaaatcgta tatatgagct
     4621 atactttaac attatgagag aaaagcataa aacaaaaata ggtagttctt ggcttttaac
     4681 attaatgcaa atcatgcaga atttgagtta taaatttaaa tataaattga ccattgatga
     4741 tatccagttt ttcatttttt acctgtattg cattttcccc ctagagaaat agatcaaaag
     4801 agcacaagag tatgcgtaca tagtttacca ggtagtagaa gtgtgttaaa atgttcctgt
     4861 aaaagaaaca ttggtaaatt taaatacata ctgttctaat tttgtatttt ttaatttttg
     4921 aatttgacat tgagtttaat tcagcaacaa caaaaaatac atataaaact agaaagggac
     4981 tttttttcct ttcttttcat aatgaagcag atcaacttaa aggataataa aatttttaaa
     5041 gaaaagatat tctaatgtac tctcaataat tctacagaaa taaaactgta aagtgcaatg
     5101 tgaaatcaaa gattatagtc attgtataat ttggcttgga ggctatgaaa tgtctttttt
     5161 tctttttggt attttacatt attcacattt tagaataaca agaacaccaa gaattacccc
     5221 taaaacagag accctgtatt taatctactt tgatcagaga agtagaattt ataacaggtt
     5281 agctaaaatt gggagcatgc cttaaaactt aaagattgta tgcatatatg tgtatatgtt
     5341 ataaacgtga aatatatttg acacacacat tcacatataa attgttaaaa actgaaggca
     5401 gaatggaact aatatatgta acagagaaaa acaataaatt ttatgaactt gttttatatt
     5461 tgcatatcaa gagtcaagta tgatgtttct ttaagttgac tttttttact tcattatttt
     5521 taggaataaa tgtaagattt tacaaatctt ttatttcccc acaagatctg aagtttggta
     5581 tttttgcatt atgacagttg ttgagactag gattttaagc taggatatga ttatatttcc
     5641 tatataacta aaaattttgt ttcataaatt ttaaaataat tatttttgac tatgaacatt
     5701 agtccaaatt taatatttga cacagttcat accagcttgc tacaataatg ataatttatt
     5761 agtctttctg ttatttaaag aataaaaaca tgcttataaa agacttttaa tgaaatgttg
     5821 cctttttaaa ataattatac ttgcacatga aaataaaata taaagtcaat aatagtcctt
     5881 gtagcccaat gggaattgat tctgtttatt gtctgtacca ttttgctacc agttacattg
     5941 aactgcttta aaataaataa taaaattatt tctaatgatg aaaa
//
