LOCUS       NM_019842               6364 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens potassium voltage-gated channel subfamily Q member 5
            (KCNQ5), transcript variant 1, mRNA.
ACCESSION   NM_019842
VERSION     NM_019842.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Kruger,J., Schubert,J., Kegele,J., Labalme,A., Mao,M., Heighway,J.,
            Seebohm,G., Yan,P., Koko,M., Aslan-Kara,K., Caglayan,H.,
            Steinhoff,B.J., Weber,Y.G., Keo-Kosal,P., Berkovic,S.F.,
            Hildebrand,M.S., Petrou,S., Krause,R., May,P., Lesca,G.,
            Maljevic,S. and Lerche,H.
  TITLE     Loss-of-function variants in the KCNQ5 gene are implicated in
            genetic generalized epilepsies
  JOURNAL   EBioMedicine 84, 104244 (2022)
   PUBMED   36088682
  REMARK    GeneRIF: Loss-of-function variants in the KCNQ5 gene are implicated
            in genetic generalized epilepsies.
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Wei,A.D., Wakenight,P., Zwingman,T.A., Bard,A.M., Sahai,N.,
            Willemsen,M.H., Schelhaas,H.J., Stegmann,A.P.A., Verhoeven,J.S., de
            Man,S.A., Wessels,M.W., Kleefstra,T., Shinde,D.N., Helbig,K.L.,
            Basinger,A., Wagner,V.F., Rodriguez-Buritica,D., Bryant,E.,
            Millichap,J.J., Millen,K.J., Dobyns,W.B., Ramirez,J.M. and
            Kalume,F.K.
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual
            disability
  JOURNAL   J Neurophysiol 128 (1), 40-61 (2022)
   PUBMED   35583973
  REMARK    GeneRIF: Human KCNQ5 de novo mutations underlie epilepsy and
            intellectual disability.
REFERENCE   3  (bases 1 to 6364)
  AUTHORS   Nappi,M., Barrese,V., Carotenuto,L., Lesca,G., Labalme,A.,
            Ville,D., Smol,T., Rama,M., Dieux-Coeslier,A.,
            Rivier-Ringenbach,C., Soldovieri,M.V., Ambrosino,P., Mosca,I.,
            Pusch,M., Miceli,F. and Taglialatela,M.
  TITLE     Gain of function due to increased opening probability by two KCNQ5
            pore variants causing developmental and epileptic encephalopathy
  JOURNAL   Proc Natl Acad Sci U S A 119 (15), e2116887119 (2022)
   PUBMED   35377796
  REMARK    GeneRIF: Gain of function due to increased opening probability by
            two KCNQ5 pore variants causing developmental and epileptic
            encephalopathy.
REFERENCE   4  (bases 1 to 6364)
  AUTHORS   Serrano-Novillo,C., Oliveras,A., Ferreres,J.C., Condom,E. and
            Felipe,A.
  TITLE     Remodeling of Kv7.1 and Kv7.5 Expression in Vascular Tumors
  JOURNAL   Int J Mol Sci 21 (17), 6019 (2020)
   PUBMED   32825637
  REMARK    GeneRIF: Remodeling of Kv7.1 and Kv7.5 Expression in Vascular
            Tumors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6364)
  AUTHORS   Brueggemann,L.I., Cribbs,L.L. and Byron,K.L.
  TITLE     Structural Determinants of Kv7.5 Potassium Channels That Confer
            Changes in Phosphatidylinositol 4,5-Bisphosphate (PIP2) Affinity
            and Signaling Sensitivities in Smooth Muscle Cells
  JOURNAL   Mol Pharmacol 97 (3), 145-158 (2020)
   PUBMED   31871302
  REMARK    GeneRIF: Distinct functional domains were identified that confer
            differential sensitivities of Kv7.5 and Kv7.4 to stimulatory and
            inhibitory signaling.
REFERENCE   6  (bases 1 to 6364)
  AUTHORS   Ozeki,Y., Tomoda,T., Kleiderlein,J., Kamiya,A., Bord,L., Fujii,K.,
            Okawa,M., Yamada,N., Hatten,M.E., Snyder,S.H., Ross,C.A. and
            Sawa,A.
  TITLE     Disrupted-in-Schizophrenia-1 (DISC-1): mutant truncation prevents
            binding to NudE-like (NUDEL) and inhibits neurite outgrowth
  JOURNAL   Proc Natl Acad Sci U S A 100 (1), 289-294 (2003)
   PUBMED   12506198
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13969]
REFERENCE   7  (bases 1 to 6364)
  AUTHORS   Yus-Najera,E., Santana-Castro,I. and Villarroel,A.
  TITLE     The identification and characterization of a noncontinuous
            calmodulin-binding site in noninactivating voltage-dependent KCNQ
            potassium channels
  JOURNAL   J Biol Chem 277 (32), 28545-28553 (2002)
   PUBMED   12032157
REFERENCE   8  (bases 1 to 6364)
  AUTHORS   Wickenden,A.D., Zou,A., Wagoner,P.K. and Jegla,T.
  TITLE     Characterization of KCNQ5/Q3 potassium channels expressed in
            mammalian cells
  JOURNAL   Br J Pharmacol 132 (2), 381-384 (2001)
   PUBMED   11159685
REFERENCE   9  (bases 1 to 6364)
  AUTHORS   Schroeder,B.C., Hechenberger,M., Weinreich,F., Kubisch,C. and
            Jentsch,T.J.
  TITLE     KCNQ5, a novel potassium channel broadly expressed in brain,
            mediates M-type currents
  JOURNAL   J Biol Chem 275 (31), 24089-24095 (2000)
   PUBMED   10816588
REFERENCE   10 (bases 1 to 6364)
  AUTHORS   Lerche,C., Scherer,C.R., Seebohm,G., Derst,C., Wei,A.D., Busch,A.E.
            and Steinmeyer,K.
  TITLE     Molecular cloning and functional expression of KCNQ5, a potassium
            channel subunit that may contribute to neuronal M-current diversity
  JOURNAL   J Biol Chem 275 (29), 22395-22400 (2000)
   PUBMED   10787416
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from FO393414.1, AF249278.1,
            AK299550.1 and AL365232.24.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 19, 2019 this sequence version replaced NM_019842.3.
            
            Summary: This gene is a member of the KCNQ potassium channel gene
            family that is differentially expressed in subregions of the brain
            and in skeletal muscle. The protein encoded by this gene yields
            currents that activate slowly with depolarization and can form
            heteromeric channels with the protein encoded by the KCNQ3 gene.
            Currents expressed from this protein have voltage dependences and
            inhibitor sensitivities in common with M-currents. They are also
            inhibited by M1 muscarinic receptor activation. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, May 2009].
            
            Transcript Variant: This variant (1) lacks an alternate in-frame
            exon in the central coding region, compared to variant 4. The
            resulting isoform (1) lacks an internal segment, compared to
            isoform 4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2575239.1, AF202977.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370398.6/ ENSP00000359425.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-407               FO393414.1         113690-114096
            408-524             AF249278.1         391-507
            525-528             AK299550.1         346-349
            529-3091            AF249278.1         512-3074
            3092-6364           AL365232.24        62470-65742
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q13"
     gene            1..6364
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="potassium voltage-gated channel subfamily Q member
                     5"
                     /db_xref="GeneID:56479"
                     /db_xref="HGNC:HGNC:6299"
                     /db_xref="MIM:607357"
     exon            1..524
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..2925
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="isoform 1 is encoded by transcript variant 1;
                     potassium channel protein; KQT-like 5; potassium channel
                     subunit alpha KvLQT5; voltage-gated potassium channel
                     subunit Kv7.5; potassium channel, voltage gated KQT-like
                     subfamily Q, member 5"
                     /codon_start=1
                     /product="potassium voltage-gated channel subfamily KQT
                     member 5 isoform 1"
                     /protein_id="NP_062816.2"
                     /db_xref="CCDS:CCDS4976.1"
                     /db_xref="GeneID:56479"
                     /db_xref="HGNC:HGNC:6299"
                     /db_xref="MIM:607357"
                     /translation="MPRHHAGGEEGGAAGLWVKSGAAAAAAGGGRLGSGMKDVESGRG
                     RVLLNSAAARGDGLLLLGTRAATLGGGGGGLRESRRGKQGARMSLLGKPLSYTSSQSC
                     RRNVKYRRVQNYLYNVLERPRGWAFIYHAFVFLLVFGCLILSVFSTIPEHTKLASSCL
                     LILEFVMIVVFGLEFIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDTIVLIASIAVVS
                     AKTQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLV
                     LIFSSFLVYLVEKDANKEFSTYADALWWGTITLTTIGYGDKTPLTWLGRLLSAGFALL
                     GISFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAANLIQCVWRSYAADEKSVSIAT
                     WKPHLKALHTCSPTKKEQGEASSSQKLSFKERVRMASPRGQSIKSRQASVGDRRSPST
                     DITAEGSPTKVQKSWSFNDRTRFRPSLRLKSSQPKPVIDADTALGTDDVYDEKGCQCD
                     VSVEDLTPPLKTVIRAIRIMKFHVAKRKFKETLRPYDVKDVIEQYSAGHLDMLCRIKS
                     LQTRVDQILGKGQITSDKKSREKITAEHETTDDLSMLGRVVKVEKQVQSIESKLDCLL
                     DIYQQVLRKGSASALALASFQIPPFECEQTSDYQSPVDSKDLSGSAQNSGCLSRSTSA
                     NISRGLQFILTPNEFSAQTFYALSPTMHSQATQVPISQSDGSAVAATNTIANQINTAP
                     KPAAPTTLQIPPPLPAIKHLPRPETLHPNPAGLQESISDVTTCLVASKENVQVAQSNL
                     TKDRSMRKSFDMGGETLLSVCPMVPKDLGKSLSVQNLIRSTEELNIQLSGSESSGSRG
                     SQDFYPKWRESKLFITDEEVGPEETETDTFDAAPQPAREAAFASDSLRTGRSRSSQSI
                     CKAGESTDALSLPHVKLK"
     exon            525..615
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            616..742
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            743..918
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1044
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1155
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1251
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1346
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1373
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1594
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1703
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1835
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1962
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..6364
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6249..6254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="hexamer: AATAAA"
     polyA_site      6285
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="major polyA site"
     regulatory      6343..6348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="hexamer: ATTAAA"
     polyA_site      6364
                     /gene="KCNQ5"
                     /gene_synonym="Kv7.5; MRD46"
                     /note="major polyA site"
ORIGIN      
        1 agaggtctct ggctggcggg cgccccgtcg gccgccggct tcctccttga aacccgccgg
       61 cgcacatgag gccgctgccc ccgccgcagg cgctggcggc cccctcgcgg tgcccgtggt
      121 gatgccatgc cccgccacca cgcgggagga gaggagggcg gcgccgccgg gctctgggtg
      181 aagagcggcg cagcggcggc ggcggcgggc ggggggcgct tgggcagcgg catgaaggat
      241 gtggagtccg gccggggcag ggtgctgctg aactcggcag ccgccagggg cgacggcctg
      301 ctactgctgg gcacccgcgc ggccacgctc ggtggcggcg gcggtggcct gagggagagc
      361 cgccggggca agcagggggc ccggatgagc ctgctgggga agccgctctc ttacacgagt
      421 agccagagct gccggcgcaa cgtcaagtac cggcgggtgc agaactacct gtacaacgtg
      481 ctggagagac cccgcggctg ggcgttcatc taccacgctt tcgtttttct ccttgtcttt
      541 ggttgcttga ttttgtcagt gttttctacc atccctgagc acacaaaatt ggcctcaagt
      601 tgcctcttga tcctggagtt cgtgatgatt gtcgtctttg gtttggagtt catcattcga
      661 atctggtctg cgggttgctg ttgtcgatat agaggatggc aaggaagact gaggtttgct
      721 cgaaagccct tctgtgttat agataccatt gttcttatcg cttcaatagc agttgtttct
      781 gcaaaaactc agggtaatat ttttgccacg tctgcactca gaagtctccg tttcctacag
      841 atcctccgca tggtgcgcat ggaccgaagg ggaggcactt ggaaattact gggttcagtg
      901 gtttatgctc acagcaagga attaatcaca gcttggtaca taggattttt ggttcttatt
      961 ttttcgtctt tccttgtcta tctggtggaa aaggatgcca ataaagagtt ttctacatat
     1021 gcagatgctc tctggtgggg cacaattaca ttgacaacta ttggctatgg agacaaaact
     1081 cccctaactt ggctgggaag attgctttct gcaggctttg cactccttgg catttctttc
     1141 tttgcacttc ctgccggcat tcttggctca ggttttgcat taaaagtaca agaacaacac
     1201 cgccagaaac actttgagaa aagaaggaac ccagctgcca acctcattca gtgtgtttgg
     1261 cgtagttacg cagctgatga gaaatctgtt tccattgcaa cctggaagcc acacttgaag
     1321 gccttgcaca cctgcagccc taccaagaaa gaacaagggg aagcatcaag cagtcagaag
     1381 ctaagtttta aggagcgagt gcgcatggct agccccaggg gccagagtat taagagccga
     1441 caagcctcag taggtgacag gaggtcccca agcaccgaca tcacagccga gggcagtccc
     1501 accaaagtgc agaagagctg gagcttcaac gaccgaaccc gcttccggcc ctcgctgcgc
     1561 ctcaaaagtt ctcagccaaa accagtgata gatgctgaca cagcccttgg cactgatgat
     1621 gtatatgatg aaaaaggatg ccagtgtgat gtatcagtgg aagacctcac cccaccactt
     1681 aaaactgtca ttcgagctat cagaattatg aaatttcatg ttgcaaaacg gaagtttaag
     1741 gaaacattac gtccatatga tgtaaaagat gtcattgaac aatattctgc tggtcatctg
     1801 gacatgttgt gtagaattaa aagccttcaa acacgtgttg atcaaattct tggaaaaggg
     1861 caaatcacat cagataagaa gagccgagag aaaataacag cagaacatga gaccacagac
     1921 gatctcagta tgctcggtcg ggtggtcaag gttgaaaaac aggtacagtc catagaatcc
     1981 aagctggact gcctactaga catctatcaa caggtccttc ggaaaggctc tgcctcagcc
     2041 ctcgctttgg cttcattcca gatcccacct tttgaatgtg aacagacatc tgactatcaa
     2101 agccctgtgg atagcaaaga tctttcgggt tccgcacaaa acagtggctg cttatccaga
     2161 tcaactagtg ccaacatctc gagaggcctg cagttcattc tgacgccaaa tgagttcagt
     2221 gcccagactt tctacgcgct tagccctact atgcacagtc aagcaacaca ggtgccaatt
     2281 agtcaaagcg atggctcagc agtggcagcc accaacacca ttgcaaacca aataaatacg
     2341 gcacccaagc cagcagcccc aacaacttta cagatcccac ctcctctccc agccatcaag
     2401 catctgccca ggccagaaac tctgcaccct aaccctgcag gcttacagga aagcatttct
     2461 gacgtcacca cctgccttgt tgcctccaag gaaaatgttc aggttgcaca gtcaaatctc
     2521 accaaggacc gttctatgag gaaaagcttt gacatgggag gagaaactct gttgtctgtc
     2581 tgtcccatgg tgccgaagga cttgggcaaa tctttgtctg tgcaaaacct gatcaggtcg
     2641 accgaggaac tgaatataca actttcaggg agtgagtcaa gtggctccag aggcagccaa
     2701 gatttttacc ccaaatggag ggaatccaaa ttgtttataa ctgatgaaga ggtgggtccc
     2761 gaagagacag agacagacac ttttgatgcc gcaccgcagc ctgccaggga agctgccttt
     2821 gcatcagact ctctaaggac tggaaggtca cgatcatctc agagcatttg taaggcagga
     2881 gaaagtacag atgccctcag cttgcctcat gtcaaactga aataagttct tcattttctt
     2941 tccaggcata gcagttcttt agccatacat atcattgcat gaactatttc gaaagccctt
     3001 ctaaaaagtt gaaattgcaa gaatcgggaa gaacatgaaa ggcagtttat aagcccgtta
     3061 ccttttaatt gcatgaaaat gcatgtttag ggatggctaa aattccaagg tgcatcgaca
     3121 ttaacccact catttagtaa tgtaccttga gttaaaaagc ctgagaaacc aaacacagct
     3181 aatgctatgg ggtgtatgaa tatgtcaagt ttaggtcatt tagaagattt gacactgtat
     3241 tttgaaatta tgggagtaaa caccttcaaa tttcaggcat ttctgctttg tgactaaata
     3301 caaactacat tttcaagatt aggccataat gtatatttaa acacaatggc tatcaacagc
     3361 tgctaataag gtatcaacta aagcagaatt ggggaataat agaaatggct gcttatttca
     3421 agatatattt gccaacccat tcctattcag tcattttatt attaatgtaa tttgaatgtc
     3481 aatttgtgtg cttttggtga tttagcgctg tggcaagcaa ttttgcacat cattttcatg
     3541 ttgttcttta tgacaagaat gttcttcaat tagaaaatgt gcaaataatg aaattcaggg
     3601 ccagtgaggc aaatagacta tctgacatat ttgactttat gaaaacatat tgcctgatgg
     3661 cagaatcaac tttataagtg gtcaacttct acacaagcgt atgaaatact ggtcagtaga
     3721 acagccattg tgattggact ggtttctctg caatggcgcc aaccccaggc ttgccaatac
     3781 tgcctatgta aagggcaagt gtgagaagct attctcattt cgctgacata caggtaggac
     3841 tatgggggat gggacatttg agtgggactg agataggaaa ggcttgaaaa gaacccagaa
     3901 acaccaccag gaagttggca aagtaaaaga aaatgacttc cccctcaaag ggcaatgaga
     3961 gggagagaaa caaaccaaaa tagaagaact agacttttta gaaaatgagt attgctaggg
     4021 aattcaacta cctaatcttc ccttattctt atatataagc agagaatttt tgcaaggtat
     4081 ttatttttta atatgccctg aatgtctttt gctattatgt gtacattttg catatgaaag
     4141 tctaaaacga aacttccttt actttttata ctgtagtgaa aattttctat tcttcccaag
     4201 aatgttgtcc caaatctgaa attactggtt caatttcctg atataaacat ttacaattag
     4261 aagctagata gtactggcag agtctgcaaa tcagaacact taaatatatc atggcagcaa
     4321 aaagaccaag ggaaacaaaa tgacaaatgt atttatcaga aagcaagcaa tgctgactgc
     4381 ttgttagaaa cccatctagc tatcttaggt caccagaact tcagccaacg taatgacatt
     4441 caaaaaaaaa ggacataaga gatagcattt caattcaatt aaaccctagc cacaggagga
     4501 ctcaagaaag gaagttgttt ccttggcaga cataaatgct gcctccagaa gtacttctcc
     4561 agcagccaag cagaatgagc agggttatca agtggatgtt caaaggaaga aggaggccct
     4621 gaactccacc aatcctcccc agccctcccc aacaggcttt ctccatcatt gactctcccc
     4681 tgcttttctt cttcattaac tccttattca ttccctgcca gtattttgct tctgaacttc
     4741 accaggcctt aaccagcctc catccccagg tcaaccagtt ctccgcttta cctaaaatgt
     4801 gcttctcaac cttttagaac cttgtccctt ttgataaaca tggaaatttt taaagcttcc
     4861 tcttcgcatc ccacttggat tctaagacaa ttagaatcat ttagaatctt tttggctgct
     4921 ggaaatgtat agaagataga ggctaaattt tacacttcag ttaagacatt gtcaatcctt
     4981 ttaagcaatt gttttcattt tcaaaataca gaatgttagt aaattatgaa aatcaatttt
     5041 tcatcttcat tcaacctgaa gttgtgattc ccccttgcaa gaatgttgca tacacacaca
     5101 cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacccctcca
     5161 ctgacctaaa gccagacacc tgccgtgact gggcaggctc tctcccatta ttaacagagg
     5221 aagaggaggg aggcagcaac tttcaggtag cactgattct tggacatact ctgtcctggg
     5281 tcaaacccca ctggcagcag gaattctgaa tgtattgggg acccctcctt aggcctcgtt
     5341 aagtctcaaa cacagtacag ctcacccagc attgctgacg actgaagatt cagaaacaaa
     5401 acctacacac agcactgagg tcttaatcat agcaaaatgt gataaaccat gttgtgaaaa
     5461 acttttatac attgaagcca tgaccatttt gtgtctatct tactctagca caaaaattgg
     5521 gggatgttaa aagatagttg tgtggaagct ataaggttct gtctgtcgtg ttactctctg
     5581 tggaaatgag aggggtctcc catttacact aagcaaattt ccattggtca attttaatga
     5641 tgtcttgaag cacaatttag ctgagcactg agtagccata tgctttctga gtacaagtgt
     5701 gtctgccttc ttcaacatgt agggttgatc aagctaatac ttaattgcaa atatcccttg
     5761 tatgaggtta actaaaaaaa ttttgctatg tctgatctac atgtttgcat tttgttcaac
     5821 taaattaata tttgtagatt gcaagttttg tttaaaataa ctttattgag tttttaagtc
     5881 ctgatctgtt ctaaggtgcc tttctcacct cccattgatt cagtgattct gaagttctta
     5941 atttgcaagt aaaataagtc tactagagag gaggaaatca ggcacaaatt gaccaattct
     6001 catgccattt gcaaagcaaa actgtaagga tgatgaaacc tggctaacta aattaatgga
     6061 tcatttcact aaatcagata gaggaaatat cataaatatt aacttgtctc cctagaagct
     6121 gagatttttc gccttaaatg acatggttgt gtttttgtaa gagaaactta atctaatcta
     6181 ataatgtgca tttaagtaag cagttctaag tcatgtatga caatgcaatt gtctgtttcc
     6241 tgaaaacaaa taaatcggga acaccatatc catttcaagc tatcattaaa gtgtaatttc
     6301 ctttgctctc ttttactggg attatttgtt ttaaagtaaa acattaaaaa gatgtctata
     6361 aaca
//
