LOCUS       NM_004519              11583 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens potassium voltage-gated channel subfamily Q member 3
            (KCNQ3), transcript variant 1, mRNA.
ACCESSION   NM_004519
VERSION     NM_004519.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Jiang,S., Liu,B., Lin,K., Li,L., Li,R., Tan,S., Zhang,X., Jiang,L.,
            Ni,H., Wang,Y., Ding,H., Hu,J., Qian,H. and Ge,R.
  TITLE     Impacted spike frequency adaptation associated with reduction of
            KCNQ2/3 exacerbates seizure activity in temporal lobe epilepsy
  JOURNAL   Hippocampus 34 (2), 58-72 (2024)
   PUBMED   38049972
  REMARK    GeneRIF: Impacted spike frequency adaptation associated with
            reduction of KCNQ2/3 exacerbates seizure activity in temporal lobe
            epilepsy.
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   Foley,K., Shorthouse,D., Rahrmann,E., Zhuang,L., Devonshire,G.,
            Gilbertson,R.J., Fitzgerald,R.C. and Hall,B.A.
  CONSRTM   OCCAMS consortium
  TITLE     SMAD4 and KCNQ3 alterations are associated with lymph node
            metastases in oesophageal adenocarcinoma
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166867 (2024)
   PUBMED   37648039
  REMARK    GeneRIF: SMAD4 and KCNQ3 alterations are associated with lymph node
            metastases in oesophageal adenocarcinoma.
REFERENCE   3  (bases 1 to 11583)
  AUTHORS   Arredondo,K., Myers,C., Hansen-Kiss,E., Mathew,M.T., Jayaraman,V.,
            Siemon,A., Bartholomew,D., Herman,G.E. and Mori,M.
  TITLE     Phenotypic Spectrum in a Family Sharing a Heterozygous KCNQ3
            Variant
  JOURNAL   J Child Neurol 37 (6), 517-523 (2022)
   PUBMED   35384780
  REMARK    GeneRIF: Phenotypic Spectrum in a Family Sharing a Heterozygous
            KCNQ3 Variant.
REFERENCE   4  (bases 1 to 11583)
  AUTHORS   Miyake,N., Heydari,S., Garshasbi,M., Saitoh,S., Nasiri,J.,
            Hamanaka,K., Takata,A., Matsumoto,N., Beheshti,F.H. and
            Chaleshtori,A.R.S.
  TITLE     The identification of two pathogenic variants in a family with mild
            and severe forms of developmental delay
  JOURNAL   J Hum Genet 66 (4), 445-448 (2021)
   PUBMED   33037390
  REMARK    GeneRIF: The identification of two pathogenic variants in a family
            with mild and severe forms of developmental delay.
REFERENCE   5  (bases 1 to 11583)
  AUTHORS   Wang,H.S., Pan,Z., Shi,W., Brown,B.S., Wymore,R.S., Cohen,I.S.,
            Dixon,J.E. and McKinnon,D.
  TITLE     KCNQ2 and KCNQ3 potassium channel subunits: molecular correlates of
            the M-channel
  JOURNAL   Science 282 (5395), 1890-1893 (1998)
   PUBMED   9836639
REFERENCE   6  (bases 1 to 11583)
  AUTHORS   Yang,W.P., Levesque,P.C., Little,W.A., Conder,M.L.,
            Ramakrishnan,P., Neubauer,M.G. and Blanar,M.A.
  TITLE     Functional expression of two KvLQT1-related potassium channels
            responsible for an inherited idiopathic epilepsy
  JOURNAL   J Biol Chem 273 (31), 19419-19423 (1998)
   PUBMED   9677360
REFERENCE   7  (bases 1 to 11583)
  AUTHORS   Charlier,C., Singh,N.A., Ryan,S.G., Lewis,T.B., Reus,B.E.,
            Leach,R.J. and Leppert,M.
  TITLE     A pore mutation in a novel KQT-like potassium channel gene in an
            idiopathic epilepsy family
  JOURNAL   Nat Genet 18 (1), 53-55 (1998)
   PUBMED   9425900
REFERENCE   8  (bases 1 to 11583)
  AUTHORS   Miceli,F., Soldovieri,M.V., Weckhuysen,S., Cooper,E.C. and
            Taglialatela,M.
  TITLE     KCNQ3-Related Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24851285
REFERENCE   9  (bases 1 to 11583)
  AUTHORS   Miceli,F., Soldovieri,M.V., Weckhuysen,S., Cooper,E. and
            Taglialatela,M.
  TITLE     KCNQ2-Related Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20437616
REFERENCE   10 (bases 1 to 11583)
  AUTHORS   Ryan,S.G., Wiznitzer,M., Hollman,C., Torres,M.C., Szekeresova,M.
            and Schneider,S.
  TITLE     Benign familial neonatal convulsions: evidence for clinical and
            genetic heterogeneity
  JOURNAL   Ann Neurol 29 (5), 469-473 (1991)
   PUBMED   1859177
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC018540.13, AF033347.1,
            AV728013.1, AC131042.6 and BX538233.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004519.3.
            
            Summary: This gene encodes a protein that functions in the
            regulation of neuronal excitability. The encoded protein forms an
            M-channel by associating with the products of the related KCNQ2 or
            KCNQ5 genes, which both encode integral membrane proteins.
            M-channel currents are inhibited by M1 muscarinic acetylcholine
            receptors and are activated by retigabine, a novel anti-convulsant
            drug. Defects in this gene are a cause of benign familial neonatal
            convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal
            type 2 (EBN2). Alternative splicing of this gene results in
            multiple transcript variants. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1). The 5' extent of
            this variant is inferred based on orthologous alignments.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF033347.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000388996.10/ ENSP00000373648.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-704               AC018540.13        87622-88325
            705-3435            AF033347.1         1-2731
            3436-3464           AV728013.1         74-102
            3465-11406          AC131042.6         108286-116227
            11407-11583         BX538233.1         4841-5017
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.22"
     gene            1..11583
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /note="potassium voltage-gated channel subfamily Q member
                     3"
                     /db_xref="GeneID:3786"
                     /db_xref="HGNC:HGNC:6297"
                     /db_xref="MIM:602232"
     exon            1..949
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     CDS             564..3182
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     potassium channel, voltage-gated, subfamily Q, member 3;
                     potassium channel subunit alpha KvLQT3; voltage-gated
                     potassium channel subunit Kv7.3; potassium voltage-gated
                     channel, KQT-like subfamily, member 3; potassium channel,
                     voltage gated KQT-like subfamily Q, member 3"
                     /codon_start=1
                     /product="potassium voltage-gated channel subfamily KQT
                     member 3 isoform 1"
                     /protein_id="NP_004510.1"
                     /db_xref="CCDS:CCDS34943.1"
                     /db_xref="GeneID:3786"
                     /db_xref="HGNC:HGNC:6297"
                     /db_xref="MIM:602232"
                     /translation="MGLKARRAAGAAGGGGDGGGGGGGAANPAGGDAAAAGDEERKVG
                     LAPGDVEQVTLALGAGADKDGTLLLEGGGRDEGQRRTPQGIGLLAKTPLSRPVKRNNA
                     KYRRIQTLIYDALERPRGWALLYHALVFLIVLGCLILAVLTTFKEYETVSGDWLLLLE
                     TFAIFIFGAEFALRIWAAGCCCRYKGWRGRLKFARKPLCMLDIFVLIASVPVVAVGNQ
                     GNVLATSLRSLRFLQILRMLRMDRRGGTWKLLGSAICAHSKELITAWYIGFLTLILSS
                     FLVYLVEKDVPEVDAQGEEMKEEFETYADALWWGLITLATIGYGDKTPKTWEGRLIAA
                     TFSLIGVSFFALPAGILGSGLALKVQEQHRQKHFEKRRKPAAELIQAAWRYYATNPNR
                     IDLVATWRFYESVVSFPFFRKEQLEAASSQKLGLLDRVRLSNPRGSNTKGKLFTPLNV
                     DAIEESPSKEPKPVGLNNKERFRTAFRMKAYAFWQSSEDAGTGDPMAEDRGYGNDFPI
                     EDMIPTLKAAIRAVRILQFRLYKKKFKETLRPYDVKDVIEQYSAGHLDMLSRIKYLQT
                     RIDMIFTPGPPSTPKHKKSQKGSAFTFPSQQSPRNEPYVARPSTSEIEDQSMMGKFVK
                     VERQVQDMGKKLDFLVDMHMQHMERLQVQVTEYYPTKGTSSPAEAEKKEDNRYSDLKT
                     IICNYSETGPPEPPYSFHQVTIDKVSPYGFFAHDPVNLPRGGPSSGKVQATPPSSATT
                     YVERPTVLPILTLLDSRVSCHSQADLQGPYSDRISPRQRRSITRDSDTPLSLMSVNHE
                     ELERSPSGFSISQDRDDYVFGPNGGSSWMREKRYLAEGETDTDTDPFTPSGSMPLSST
                     GDGISDSVWTPSNKPI"
     exon            950..1040
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1167
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1340
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1496
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1607
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1703
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1798
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1825
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..2028
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2131
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2263
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2362
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2447
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..11583
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11563..11568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /note="hexamer: AATAAA"
     polyA_site      11583
                     /gene="KCNQ3"
                     /gene_synonym="BFNC2; EBN2; KV7.3"
                     /note="major polyA site"
ORIGIN      
        1 ggcgtgctct cggcccgcgg cgcgctcgct gctgctgctg ctgacaggct gaggctggaa
       61 cgggcgggga ggggcgcggg tgggagcgcg gaggagggag gaggcggcgg cggcggcgcg
      121 tgtggctggc gggcgggagg gcggctggca ccgggcttgt aaataaactg cgatgctgtc
      181 ttgggctcgc cggcctgggt gccgccgccg ccgccgccgc cccggagcgc cggttccagg
      241 ctcccgccgc cgcgcgccct agcgcctccg cgccagcctc gcgcgccctc ccagcttctc
      301 cgcagccccc ggcccgcgac cctcggggtc ctgcggctga cccgcgcggg atgtgacccc
      361 ctgaccccct gcctggcctc ccctgccccc cagggggccc gcctttgcct gcttttgggg
      421 gggggtgggg aggggcgcgc ggatcatggc attggagttc ccgggcttgc agccgccgcc
      481 gccgcctcgt tcacgcaccc cgagcgcccc ttcttcccag agcagcagcg gagaaggcga
      541 agcggccggc gggggcgagg cagatggggc tcaaggcgcg cagggcggcg ggggcggctg
      601 gcggcggcgg cgacgggggc ggcggaggcg gcggggcggc taacccagcc ggaggggacg
      661 cggcggcggc cggcgacgag gagcggaaag tggggctggc gcccggcgac gtggagcaag
      721 tcaccttggc gctcggggcc ggagccgaca aagacgggac cctgctgctg gagggcggcg
      781 gccgcgacga ggggcagcgg aggaccccgc agggcatcgg gctcctggcc aagaccccgc
      841 tgagccgccc agtcaagaga aacaacgcca agtaccggcg catccaaact ttgatctacg
      901 acgccctgga gagaccgcgg ggctgggcgc tgctttacca cgcgttggtg ttcctgattg
      961 tcctggggtg cttgattctg gctgtcctga ccacattcaa ggagtatgag actgtctcgg
     1021 gagactggct tctgttactg gagacatttg ctattttcat ctttggagcc gagtttgctt
     1081 tgaggatctg ggctgctgga tgttgctgcc gatacaaagg ctggcggggc cgactgaagt
     1141 ttgccaggaa gcccctgtgc atgttggaca tctttgtgct gattgcctct gtgccagtgg
     1201 ttgctgtggg aaaccaaggc aatgttctgg ccacctccct gcgaagcctg cgcttcctgc
     1261 agatcctgcg catgctgcgg atggaccgga gaggtggcac ctggaagctt ctgggctcag
     1321 ccatctgtgc ccacagcaaa gaactcatca cggcctggta catcggtttc ctgacactca
     1381 tcctttcttc atttcttgtc tacctggttg agaaagacgt cccagaggtg gatgcacaag
     1441 gagaggagat gaaagaggag tttgagacct atgcagatgc cctgtggtgg ggcctgatca
     1501 cactggccac cattggctat ggagacaaga cacccaaaac gtgggaaggc cgtctgattg
     1561 ccgccacctt ttccttaatt ggcgtctcct tttttgccct tccagcgggc atcctggggt
     1621 ccgggctggc cctcaaggtg caggagcaac accgtcagaa gcactttgag aaaaggagga
     1681 agccagctgc tgagctcatt caggctgcct ggaggtatta tgctaccaac cccaacagga
     1741 ttgacctggt ggcgacatgg agattttatg aatcagtcgt ctcttttcct ttcttcagga
     1801 aagaacagct ggaggcagca tccagccaaa agctgggtct cttggatcgg gttcgccttt
     1861 ctaatcctcg tggtagcaat actaaaggaa agctatttac ccctctgaat gtagatgcca
     1921 tagaagaaag tccttctaaa gaaccaaagc ctgttggctt aaacaataaa gagcgtttcc
     1981 gcacggcctt ccgcatgaaa gcctacgctt tctggcagag ttctgaagat gccgggacag
     2041 gtgaccccat ggcggaagac aggggctatg ggaatgactt ccccatcgaa gacatgatcc
     2101 ccaccctgaa ggccgccatc cgagccgtca gaattctaca attccgtctc tataaaaaaa
     2161 aattcaagga gactttgagg ccttacgatg tgaaggatgt gattgagcag tattctgccg
     2221 ggcatctcga catgctttcc aggataaagt accttcagac gagaatagat atgattttca
     2281 cccctggacc tccctccacg ccaaaacaca agaagtctca gaaagggtca gcattcacct
     2341 tcccatccca gcaatctccc aggaatgaac catatgtagc cagaccatcc acatcagaaa
     2401 tcgaagacca aagcatgatg gggaagtttg taaaagttga aagacaggtt caggacatgg
     2461 ggaagaagct ggacttcctc gtggatatgc acatgcaaca catggaacgg ttgcaggtgc
     2521 aggtcacgga gtattaccca accaagggca cctcctcgcc agctgaagca gagaagaagg
     2581 aggacaacag gtattccgat ttgaaaacca tcatctgcaa ctattctgag acaggccccc
     2641 cggaaccacc ctacagcttc caccaggtga ccattgacaa agtcagcccc tatgggtttt
     2701 ttgcacatga ccctgtgaac ctgccccgag ggggacccag ttctggaaag gttcaggcaa
     2761 ctcctccttc ctcagcaaca acgtatgtgg agaggcccac ggtcctgcct atcttgactc
     2821 ttctcgactc ccgagtgagc tgccactccc aggctgacct gcagggcccc tactcggacc
     2881 gaatctcccc ccggcagaga cgtagcatca cgcgagacag tgacacacct ctgtccctga
     2941 tgtcggtcaa ccacgaggag ctggagaggt ctccaagtgg cttcagcatc tcccaggaca
     3001 gagatgatta tgtgttcggc cccaatgggg ggtcgagctg gatgagggag aagcggtacc
     3061 tcgccgaggg tgagacggac acagacacgg accccttcac gcccagcggc tccatgcctc
     3121 tgtcgtccac aggggatggg atttctgatt cagtatggac cccttccaat aagcccattt
     3181 aaaagaggtc actggctgac ccctccttgt aatgtagaca gactttgtat agttcactta
     3241 ctcttacacc cgacgcttac cagcggggac accaatggct gcatcaaatg catgcgtgtg
     3301 cgtggtggcc ccacccaggc aggggcttcc cacagcctct tcctccccat gtcaccacaa
     3361 caaagtgctt ccttttcagc atggtttgca tgactttaca ctatataaat ggttccgcta
     3421 atctcttcta ggatacacat ttatctgctg ttcttacttt taatcacgat tggaccagta
     3481 cagggagaaa ttactgatga gccatgctat ttgtctgttt ggttggctgg tttgggtttt
     3541 ggtttggtaa gcagagaaag tagtttaagt tatttctagg atcactgccc tcttgtgtaa
     3601 aacgctccag caattaactg tttttgaaag caaggaaata attttctcaa caaaaccaca
     3661 ttgtgtatca gtggggctgc ctactgagct aacattagga aaggaatgag atagtcattg
     3721 tgtctctaaa acaaagaatt taaacaaata tttaaggcaa aaccttttaa aagctctcca
     3781 aaagacctca cttggcctga tgttacttag tatcatgcct cagtttcctt atctgtaaaa
     3841 agtggtggta atcccaagtg tagcatttct gatgagttat gaattggcag ggagaaaaca
     3901 cacacacaca cacacacaca cacacacaca cacacataca caaagttaaa atagtttcca
     3961 atttatgtag ccaaggacaa tggaatttat cagaagtgaa gtgcccctct gggcaggagc
     4021 tgagttcagt gcctttaaaa tgaatttcca actctcattt cttcccatga tactgaagta
     4081 caaaatatcc atctaaaata gaaattaaag tcaagaaggc acacagaaat tttctagtcc
     4141 aaggacttcc ttttataaat gaagaatctt aggctcaggt aaagtgacat acccagagtc
     4201 actcagttgt cccatagaga accaggactc aaaagtcagg tctgcccact ctccctgacc
     4261 ttgtttcccc tcaccacacc attcttccat ttatataatg catcctagag attgtgtcaa
     4321 gatatatgac tttgtgtcgc gtggaaagca cccagtttat ccagcaggag ggggatttcc
     4381 acttttattg caaaacatgc ttgcttgatg caatgcattt cagatctaga aaaaaataat
     4441 gcaagcaagc tacatttaca gatgtgtaaa caggcaaaat ttccagaacc gtccaatttt
     4501 acttttccaa atttatggaa ccaacccaga ttttttaaat gaaaatacta aaaccatatg
     4561 taaccgtaag acaagctaaa atgtaagcag acagcctggg aagcactgcc ctactgtaca
     4621 ggtgtgatat taagcacaag ccatcctctg gcctcttatg agatgttgtt attctctgaa
     4681 aaactttgat ggaaaaggtt tctgttaggc ttagactgaa ttagctaaat gaccatgcca
     4741 cagtattatt gtatcactct tctcacacac ctaggcactg gttgtggata gttgccatgg
     4801 tgacaatcaa gataatccag atgacctaaa caaatgtctg tatgcagctg acactgctat
     4861 tctccctcac agtcttgtca agagccacag tagctatggc agacatttgg ttgatggttt
     4921 catcatttcc taatacgaga gtctggcact atgttggaga gaggaatcac ttcctcagag
     4981 ataggttcct ttcaactcac atgtcaacag ttactgtaca aaaagctcag aatacagtaa
     5041 tgattagagc ttgacccctg actttaggaa cttagaacca aagcccaaag tcccaggcca
     5101 agggcagaca ttgtgagcag cagaagggtg ttttttatgg agagccccat aaatggatgg
     5161 gtgtagacct tgaagtctga gatgggaaat ataccagaat gctcaatgca tcagacatga
     5221 agccccacgg gcttccattt gacatgctgg caatctgaag gacaaagggc atgggttatt
     5281 tgggcctttt ccccactcct tcaatatggc tgaaagtgcc agtggggatt acaacctaag
     5341 aaaccctatg ccccttcctg agggcttgat tattgaatcc tgatcttcct ggaatctttc
     5401 acttgagatg atcacctgcc taacatccaa catgttacta tgctcttctt tatgtacatg
     5461 agttactgga aaccaacaat aaaggagagt gagtcatact ctccacatat gcgccaatta
     5521 attttttaac ttacttatta gtattcttga agaacttact atcttaagta ttttaccaat
     5581 agatgatgca ttttccagca actcaatgga gttctacctt aaagtattta ccattgatcc
     5641 aaaattggag gaactacctt agattctttt aataacctag aaaggattgg agcttgtatt
     5701 atttcctaaa ccataggcag gcagtctagc ttccctgctg gatcataaga ccaggacagc
     5761 agagattggg tttgtattga tcagtagtag ggaagtattg attgtctaac atctagtaag
     5821 ttgtctttat cagtatagac atagacaaaa tacatgtttt atgaatgaat aaataataag
     5881 aaccttaaga gctgtttttt tcccatttaa gaaaacaaaa aggaaaagag aaaaatgatc
     5941 ttctggaata actctgcttt gatattctcc ttttgttccc caaaaaatat attccagaaa
     6001 ggcaaaccaa acctgctcaa gttgcctatg cacagacaat ctcaacaata tggctcacag
     6061 tgagcaagca gaaattcagg gtctggaatt gaatgtgagt tatgggaaga aacagtgtgt
     6121 gctttcacaa agatcctttt gtcagacaag ggaacagcat gataatccaa tggaactggt
     6181 taacatgacc caagatgaca tgaccacaga tatactagtc gttctgaaac ttgcccaaga
     6241 aattgcaatc gtttcagcag aactccatga aatattgaca aattaattcc atcatcaaac
     6301 taggagtgat attatcaggt aaaactatga tctcatgtat gtatttggtc tttctgggtc
     6361 acaatgtgac atgaaaaatt ttaaaggtca tattttagca agatcaaaat gaattagata
     6421 aatcaacttc acaaatgtta agcgaaacat gatcctattg agctgagaaa gaagtacttg
     6481 agatcgtttg agttttgact atcaaatgtc agcacaaaag caaatatcaa aagcttaaga
     6541 aaaaaattat ttcaggaaaa gaaaaacaca tcaaattgag aaagtgttgt tgaataactt
     6601 tgtttttaga actggaaatt taggcttatt agacatgaca ttgatattac aaagccctag
     6661 ttttcatgaa aacataacta gataacccca aggtgtatag gttaaaatga actaattaat
     6721 caacaattat gcatcctgtg tctattttgt gcaaggtact tcctagacac tgtagtttga
     6781 aagaataatt tcctgtagaa aaacctaatc taataggacc tactcataat ttggcaaaat
     6841 catcctcata attccatgct actgaaatgg gggtgtaata aagaaaaaat catttgttta
     6901 ttcatttaac agggtttatt aagcaccaat atatgcccag aactttgcag tgcactggaa
     6961 gttcagagat aagaaaacca cagtttctgc catcaaaatg ctaggaatct agaggtctca
     7021 aacatcagag gtcccttcaa actatgtcta ccaggaaaat aaaatacctg atttcatccc
     7081 tgcaaacaac tctatacaga cagaaatgct tcctagcttg gaaactatag aaaattgaac
     7141 atgaaaatga gagcaaaggt tctacatcaa aatggcaatg atccaatcag cccagtcaag
     7201 gctgagtggg ttgaatgggg acttcgagtt tggattcaat gctttgaact tcatagctcc
     7261 agagaagctg tgcaaaaagg ggggattcta gggaatctga ggaagattgt tggagaaacc
     7321 agctttcttc ttgtctccta atccctgcta agattgatac cagtcaagga gctgagttac
     7381 tttcatttca ttatgttgac caagagaagc ttatgataca gactatgaca cagcacctat
     7441 ttcaagagaa agatgttact ctacttatgg gaacctgcag atcaaattct cctggggcaa
     7501 gagaagaaat ttgttcagca gcctaagaag tcaactgtac tttggacagt gctgccaaac
     7561 acaccccagg attctcccac ccctgtgctt tcccagaaga tgccacatag ctggtatttg
     7621 gttttcatga ataaccaaag attcttcttt gcttcctgaa ccccaggcat ccttaggaat
     7681 ttgcttcatg tcctcacttg tccttgaagg cttgtttttc aggatgaact tttaaagtct
     7741 aagttgttta tattcaaatc aacccaatgc tattgttgct gatattaaac cattccaaac
     7801 tgagccaact ggcccctaaa caagccagca ggcagcccct cactaatgcc cagtttagaa
     7861 catgttagga ccaggctatc tgcatagctt tagtggtttt agaagccagt aatggcagtg
     7921 taaaagagga atcatacaga gatgacctgg aagaaaattt gagtttcagc acattcaaaa
     7981 gcatttgact cttctgccgt gttttgattt tcaagtgaga caatcaatgt acacacaaag
     8041 cctctagtac aatgactgat gcatactagg gactgttatt tatgttattt ttttacagta
     8101 tgcatggact tcattgtctc agtctttggt gttacttata aactctaaat ttacaccaaa
     8161 aaaatttttt ttcagaaaaa gaatcgtgga gaaacaatcc taattattaa atcaaatgct
     8221 ttcacaattg ctttttaaat aagtacatct ctgcaaataa aaatgcttgc tcttgtctac
     8281 cacaataaag aacagaagga atcttggaat gaagagaggc aggaatgttg gatctcagtg
     8341 gctgagttgt ggcaattcca ttggacatat agtaccagat gttgcaccat ctggatgatg
     8401 tgtaggagat aaagccaaat tgcaaaaata aaagactcat gatttcccaa ggaaagtcaa
     8461 ctgtgcatgg tggctatcag acaacaccct agcaatgaat gggggaactt gcagaagaaa
     8521 gagttaatgc ttgcttaaac cttccctggg gagatttgtt cccaggcttt cacggcttta
     8581 acctcagcaa ccctgtaaga aaattgtgat tcatattttg atttgttatc atagatgcaa
     8641 agagacataa tttcatggat tctaagatac acagagcaag aagagatttt gatcacccag
     8701 aacaaccacc tgtcttttta gatcaagaag cagaagctca gagcaaagaa catcacacca
     8761 cgtccctcag tgattgaagc agtgattgag tcacagaata aatctggaac tcaggtcttc
     8821 tgatctttgc tccagatgtt agagacaaaa ctaaaagtaa aataccaagt gaaatcaaag
     8881 catcacgatt gagcccagaa catgaaaaag aacttcctgg cccacttgag aaactgttaa
     8941 accggacata cctttgggga cttcttccct tctctggaat aagattgatg tttccatgct
     9001 gtgaaagacg atgatgttcc ttctcccaga ttcctgctgt cttcaaaagg cctagcaaaa
     9061 accactgctg ctgggtgcag ttgagaaagg gaatgaagaa caatcccatg gccatgcagg
     9121 cactcctccc ctccacctct ctgccctcca cccctctgcc ctctcctacc ttgttccttc
     9181 ttggtcctgc catgtttgga ctggttcacc tttttggccc atcacttggg ctcattcagc
     9241 aacagtcatg ccttaggttg catcctcatc tataggccaa ttaaacaggg tttcttcttg
     9301 ataaatccct atggttttga ttttaggaag tcaggcatga tttcttttgg gatctatatg
     9361 acccaaagtg gccagtttta ccaaaggtgt ccctttgcac tgggatctct gggtctcatt
     9421 tgaacacagc ttgggtcctc ttgccttgtc ccagagaatc cccatgttgt caggacataa
     9481 gcagatactc tagggccttc accaaaagct acaaagaagc agacaggact caacttagga
     9541 catcttatgg ttatgtaact aagagattat tttaggaagt aaacaggcct ctgttggctc
     9601 aaatgaacat cttcattgtt agtttccact tacattgttc tcgggataaa tcggccagaa
     9661 tttggcaggg ctccccagca gtccttgtga atccctgtgg taggatgttt cagttctcct
     9721 gagtcaggct gaagtgaaag ctcctaagag atattcagaa agaaggatgg tcaagttcta
     9781 gaacaacaat agttggccca gtaattttaa agattgtcat tctacctaag ccttcatcct
     9841 ccagatagca ctgttatggg cttaaaataa gcttcaaggg aaataatgaa tttctttgac
     9901 accgccaaaa atcggctgta tataagaata acttgagaat ggcttacaat gaggtagtca
     9961 aaagcccagc tcagtgctca ggaatgtatt tgtaattcct cttagacttg tcgaatggac
    10021 tgcaatttga tacctaagag taataaaaaa tgagaccata ttttatgtta atgttccctt
    10081 ctcttcctct gcccacaaaa gtgaggtcag ggaaggggag agaaacttat cctaagcact
    10141 ccttatgagt tgcttgtgac atgatagctc tctctaaagt tcatgcaatc ctcatcgcaa
    10201 ccccatgagg gagatgttat tgtctccatc ttatagatga ggaaactgaa gacaagacct
    10261 tgtcaaaggt cacacattgt aagaagtaga gctgcatatt caaccaaagc ccgtgtacct
    10321 ggaaagctca ttctctctcc cccttcttcc tctcaaatca ggccctagct cctccacaat
    10381 gcttcgggct tctacgttca ctcagcttta cagtaaaatg atcacagacc catgagttcc
    10441 ctggagagta gagtgatgcc tgggtcagct gttcctcttg tctgttcagc aggtcctttg
    10501 agtggcttca caatccagct gtcagtggca gaaaataatg cctcataaat tattcacctg
    10561 gttcacattg gaatccatta gctctcgggg cttggtgtgg ctgcagatca tccacaagtg
    10621 ccccttgttc tctgccccca aggagctcct gtcattgttt ttaaccaaac aagttcaaat
    10681 atctccacgt ctctgctgac tctggtcttt tgtctctaga ctgtaccttt atttctgagc
    10741 tctgaaattc tgcagaaaca ggtgagtcta taaaaacatt ctgtgggagg ccaaaagctc
    10801 agaacagttg tgtctctctt tctcagtatt aaaagatcaa ggtttcccat ggaagccaac
    10861 ctgatctggt aattgggccc ttctataccc ctctgaatac ttttccaagg agggggcagt
    10921 tcccaccccc atagttcccc tgagggttaa gagtccaggt ggtggccaag ttgcaagatt
    10981 tggggcagcc attttgccaa ggatcagaga tttgctgttg gagaggtgaa gtcggctctg
    11041 tgccatcctg aaaaatcaac ttagaaagat aggtggactt catcagattt gtcagtcctt
    11101 ccttgtttta aagagaaaaa tgatctctgc cttgcttcag ataggatatg gctatttttt
    11161 ttccttcttt ttctaatgta gcagtgatgg aaaacaccag tctttgaaga acaaatggag
    11221 gttcccagtg acatgaatcc attatatttg attttggatt gtatttttta accccttttt
    11281 atggcataga attctggtgc cttgctccct gggtttgtct ctgtgctacg agtaggcttt
    11341 cttcacattc tggcttacag gggaacacaa ctattccaca agtggccttt agtgctcttt
    11401 ataatatgat ttcctgtaat ttttaaactg tatgtgtaat ttatattctg gctctgtcca
    11461 atatttgaac aactttaata ggttgatttc catatatatt atgcaaataa ttcttacctg
    11521 attttttatg ttctatctta aataaatact gcaccactta ttaataaata gacatttcag
    11581 tga
//
