LOCUS       NM_002249              13034 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily N member
            3 (KCNN3), transcript variant 1, mRNA.
ACCESSION   NM_002249
VERSION     NM_002249.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Butler,A.S., Hancox,J.C. and Marrion,N.V.
  TITLE     Preferential formation of human heteromeric SK2:SK3 channels limits
            homomeric SK channel assembly and function
  JOURNAL   J Biol Chem 299 (1), 102783 (2023)
   PUBMED   36502918
  REMARK    GeneRIF: Preferential formation of human heteromeric SK2:SK3
            channels limits homomeric SK channel assembly and function.
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Zhang,D., Zhu,Q., Xia,W., Zhu,C., Zhao,X., Zhang,Y., He,C., Ji,S.,
            Li,X. and Zhang,J.
  TITLE     The role of SK3 in progesterone-induced inhibition of human
            fallopian tubal contraction
  JOURNAL   Reprod Biol Endocrinol 20 (1), 73 (2022)
   PUBMED   35488306
  REMARK    GeneRIF: The role of SK3 in progesterone-induced inhibition of
            human fallopian tubal contraction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13034)
  AUTHORS   Gripp,K.W., Smithson,S.F., Scurr,I.J., Baptista,J., Majumdar,A.,
            Pierre,G., Williams,M., Henderson,L.B., Wentzensen,I.M.,
            McLaughlin,H., Leeuwen,L., Simon,M.E.H., van Binsbergen,E.,
            Dinulos,M.B.P., Kaplan,J.D., McRae,A., Superti-Furga,A., Good,J.M.
            and Kutsche,K.
  TITLE     Syndromic disorders caused by gain-of-function variants in KCNH1,
            KCNK4, and KCNN3-a subgroup of K+ channelopathies
  JOURNAL   Eur J Hum Genet 29 (9), 1384-1395 (2021)
   PUBMED   33594261
  REMARK    GeneRIF: Syndromic disorders caused by gain-of-function variants in
            KCNH1, KCNK4, and KCNN3-a subgroup of K(+) channelopathies.
REFERENCE   4  (bases 1 to 13034)
  AUTHORS   Rahm,A.K., Wieder,T., Gramlich,D., Muller,M.E., Wunsch,M.N., El
            Tahry,F.A., Heimberger,T., Weis,T., Most,P., Katus,H.A., Thomas,D.
            and Lugenbiel,P.
  TITLE     HDAC2-dependent remodeling of KCa2.2 (KCNN2) and KCa2.3 (KCNN3) K+
            channels in atrial fibrillation with concomitant heart failure
  JOURNAL   Life Sci 266, 118892 (2021)
   PUBMED   33310041
  REMARK    GeneRIF: HDAC2-dependent remodeling of KCa2.2 (KCNN2) and KCa2.3
            (KCNN3) K(+) channels in atrial fibrillation with concomitant heart
            failure.
REFERENCE   5  (bases 1 to 13034)
  AUTHORS   Bery,F., Figiel,S., Kouba,S., Fontaine,D., Gueguinou,M.,
            Potier-Cartereau,M., Vandier,C., Guibon,R., Bruyere,F., Fromont,G.
            and Maheo,K.
  TITLE     Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating
            EMT-Activator Zeb1 and SK3 Channel Expression
  JOURNAL   Int J Mol Sci 21 (13), 4786 (2020)
   PUBMED   32640738
  REMARK    GeneRIF: Hypoxia Promotes Prostate Cancer Aggressiveness by
            Upregulating EMT-Activator Zeb1 and SK3 Channel Expression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13034)
  AUTHORS   Wittekindt,O.H., Visan,V., Tomita,H., Imtiaz,F., Gargus,J.J.,
            Lehmann-Horn,F., Grissmer,S. and Morris-Rosendahl,D.J.
  TITLE     An apamin- and scyllatoxin-insensitive isoform of the human SK3
            channel
  JOURNAL   Mol Pharmacol 65 (3), 788-801 (2004)
   PUBMED   14978258
REFERENCE   7  (bases 1 to 13034)
  AUTHORS   Dror,V., Shamir,E., Ghanshani,S., Kimhi,R., Swartz,M., Barak,Y.,
            Weizman,R., Avivi,L., Litmanovitch,T., Fantino,E., Kalman,K.,
            Jones,E.G., Chandy,K.G., Gargus,J.J., Gutman,G.A. and Navon,R.
  TITLE     hKCa3/KCNN3 potassium channel gene: association of longer CAG
            repeats with schizophrenia in Israeli Ashkenazi Jews, expression in
            human tissues and localization to chromosome 1q21
  JOURNAL   Mol Psychiatry 4 (3), 254-260 (1999)
   PUBMED   10395215
REFERENCE   8  (bases 1 to 13034)
  AUTHORS   Chandy,K.G., Fantino,E., Wittekindt,O., Kalman,K., Tong,L.L.,
            Ho,T.H., Gutman,G.A., Crocq,M.A., Ganguli,R., Nimgaonkar,V.,
            Morris-Rosendahl,D.J. and Gargus,J.J.
  TITLE     Isolation of a novel potassium channel gene hSKCa3 containing a
            polymorphic CAG repeat: a candidate for schizophrenia and bipolar
            disorder?
  JOURNAL   Mol Psychiatry 3 (1), 32-37 (1998)
   PUBMED   9491810
REFERENCE   9  (bases 1 to 13034)
  AUTHORS   Imbert,G., Saudou,F., Yvert,G., Devys,D., Trottier,Y.,
            Garnier,J.M., Weber,C., Mandel,J.L., Cancel,G., Abbas,N., Durr,A.,
            Didierjean,O., Stevanin,G., Agid,Y. and Brice,A.
  TITLE     Cloning of the gene for spinocerebellar ataxia 2 reveals a locus
            with high sensitivity to expanded CAG/glutamine repeats
  JOURNAL   Nat Genet 14 (3), 285-291 (1996)
   PUBMED   8896557
REFERENCE   10 (bases 1 to 13034)
  AUTHORS   Kohler,M., Hirschberg,B., Bond,C.T., Kinzie,J.M., Marrion,N.V.,
            Maylie,J. and Adelman,J.P.
  TITLE     Small-conductance, calcium-activated potassium channels from
            mammalian brain
  JOURNAL   Science 273 (5282), 1709-1714 (1996)
   PUBMED   8781233
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL390204.11, AJ251016.1,
            BC042147.1, AL606500.8 and AW135188.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002249.5.
            
            Summary: Action potentials in vertebrate neurons are followed by an
            afterhyperpolarization (AHP) that may persist for several seconds
            and may have profound consequences for the firing pattern of the
            neuron. Each component of the AHP is kinetically distinct and is
            mediated by different calcium-activated potassium channels. This
            gene belongs to the KCNN family of potassium channels. It encodes
            an integral membrane protein that forms a voltage-independent
            calcium-activated channel, which is thought to regulate neuronal
            excitability by contributing to the slow component of synaptic AHP.
            This gene contains two CAG repeat regions in the coding sequence.
            It was thought that expansion of one or both of these repeats could
            lead to an increased susceptibility to schizophrenia or bipolar
            disorder, but studies indicate that this is probably not the case.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Feb
            2011].
            
            Transcript Variant: This variant (1) encodes isoform a (also known
            as SK3).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.1321072.1,
                                           SRR14038193.4135882.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2157437
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000271915.9/ ENSP00000271915.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL390204.11        86283-86285         c
            4-558               AJ251016.1         20-574
            559-1363            AJ251016.1         590-1394
            1364-3063           BC042147.1         263-1962
            3064-12535          AL606500.8         111316-120787       c
            12536-13027         AW135188.1         12-503              c
            13028-13034         AL606500.8         110817-110823       c
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..13034
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="potassium calcium-activated channel subfamily N
                     member 3"
                     /db_xref="GeneID:3782"
                     /db_xref="HGNC:HGNC:6292"
                     /db_xref="MIM:602983"
     exon            1..1250
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..2513
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="isoform a is encoded by transcript variant 1; SKCa
                     3; potassium intermediate/small conductance
                     calcium-activated channel, subfamily N, member 3; small
                     conductance calcium-activated potassium channel 3;
                     potassium channel, calcium activated intermediate/small
                     conductance subfamily N alpha, member 3"
                     /codon_start=1
                     /product="small conductance calcium-activated potassium
                     channel protein 3 isoform a"
                     /protein_id="NP_002240.3"
                     /db_xref="CCDS:CCDS30880.1"
                     /db_xref="GeneID:3782"
                     /db_xref="HGNC:HGNC:6292"
                     /db_xref="MIM:602983"
                     /translation="MDTSGHFHDSGVGDLDEDPKCPCPSSGDEQQQQQQQQQQQQPPP
                     PAPPAAPQQPLGPSLQPQPPQLQQQQQQQQQQQQQQPPHPLSQLAQLQSQPVHPGLLH
                     SSPTAFRAPPSSNSTAILHPSSRQGSQLNLNDHLLGHSPSSTATSGPGGGSRHRQASP
                     LVHRRDSNPFTEIAMSSCKYSGGVMKPLSRLSASRRNLIEAETEGQPLQLFSPSNPPE
                     IVISSREDNHAHQTLLHHPNATHNHQHAGTTASSTTFPKANKRKNQNIGYKLGHRRAL
                     FEKRKRLSDYALIFGMFGIVVMVIETELSWGLYSKDSMFSLALKCLISLSTIILLGLI
                     IAYHTREVQLFVIDNGADDWRIAMTYERILYISLEMLVCAIHPIPGEYKFFWTARLAF
                     SYTPSRAEADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFV
                     MKTLMTICPGTVLLVFSISLWIIAAWTVRVCERYHDQQDVTSNFLGAMWLISITFLSI
                     GYGDMVPHTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTK
                     RIKNAAANVLRETWLIYKHTKLLKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLSDQ
                     ANTLVDLSKMQNVMYDLITELNDRSEDLEKQIGSLESKLEHLTASFNSLPLLIADTLR
                     QQQQQLLSAIIEARGVSVAVGTTHTPISDSPIGVSSTSFPTPYTSSSSC"
     exon            1251..1346
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1765
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1907
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2018
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2146
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2216
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..13034
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12997..13002
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="hexamer: AACAAG"
     regulatory      13013..13018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="hexamer: ACTAAA"
     polyA_site      13027
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="major polyA site"
     polyA_site      13034
                     /gene="KCNN3"
                     /gene_synonym="hSK3; KCa2.3; SK3; SKCA3; ZLS3"
                     /note="major polyA site"
ORIGIN      
        1 attgagccag cgaggagtga agctgagcct ggcctcacac gctcctagag gaccacctcc
       61 tgagagagtt ctttcacccc ctcttctttc tccaagctcc cctcctgctc tccctccctg
      121 cccaatacaa tgcattcttg agtggcagcg tctggactcc aggcagcccc agagaaccga
      181 agcaagccaa agagaggact ggagccaaga tactggtggg ggagattgga tgcctggctt
      241 tctttgagga catctttgga gcgagggtgg ctttggggtg ggggcttgtg ctgcagggaa
      301 tacagccagg ccccaagatg gacacttctg ggcacttcca tgactcgggg gtgggggact
      361 tggatgaaga ccccaagtgc ccctgtccat cctctgggga tgagcagcag cagcagcagc
      421 agcagcaaca gcagcagcag ccaccaccgc cagcgccacc agcagccccc cagcagcccc
      481 tgggaccctc gctgcagcct cagcctccgc agcttcagca gcagcagcag cagcagcagc
      541 agcagcagca gcagcagcca ccgcatcccc tgtctcagct cgcccaactc cagagccagc
      601 ccgtccaccc tggcctgctg cactcctctc ccaccgcttt cagggccccc ccttcgtcca
      661 actccaccgc catcctccac ccttcctcca ggcaaggcag ccagctcaat ctcaatgacc
      721 acttgcttgg ccactctcca agttccacag ctacaagtgg gcctggcgga ggcagccggc
      781 accgacaggc cagccccctg gtgcaccggc gggacagcaa ccccttcacg gagatcgcca
      841 tgagctcctg caagtatagc ggtggggtca tgaagcccct cagccgcctc agcgcctccc
      901 ggaggaacct catcgaggcc gagactgagg gccaacccct ccagcttttc agccctagca
      961 accccccgga gatcgtcatc tcctcccggg aggacaacca tgcccaccag accctgctcc
     1021 atcaccctaa tgccacccac aaccaccagc atgccggcac caccgccagc agcaccacct
     1081 tccccaaagc caacaagcgg aaaaaccaaa acattggcta taagctggga cacaggaggg
     1141 ccctgtttga aaagagaaag cgactgagtg actatgctct gatttttggg atgtttggaa
     1201 ttgttgttat ggtgatagag accgagctct cttggggttt gtactcaaag gactccatgt
     1261 tttcgttggc cctgaaatgc cttatcagtc tgtccaccat catccttttg ggcttgatca
     1321 tcgcctacca cacacgtgaa gtccagctct tcgtgatcga caatggcgcg gatgactggc
     1381 ggatagccat gacctacgag cgcatcctgt acatcagcct ggagatgctg gtgtgcgcca
     1441 tccaccccat tcctggcgag tacaagttct tctggacggc acgcctggcc ttctcctaca
     1501 caccctcccg ggcggaggcc gatgtggaca tcatcctgtc tatccccatg ttcctgcgcc
     1561 tgtacctgat cgcccgagtc atgctgctgc acagcaagct cttcaccgat gcctcgtccc
     1621 gcagcatcgg ggccctcaac aagatcaact tcaacacccg ctttgtcatg aagacgctca
     1681 tgaccatctg ccctggcact gtgctgctcg tgttcagcat ctctctgtgg atcattgctg
     1741 cctggaccgt ccgtgtctgt gaaaggtacc atgaccagca ggacgtaact agtaactttc
     1801 tgggtgccat gtggctcatc tccatcacat tcctttccat tggttatggg gacatggtgc
     1861 cccacacata ctgtgggaaa ggtgtctgtc tcctcactgg catcatgggt gcaggctgca
     1921 ctgcccttgt ggtggccgtg gtggcccgaa agctggaact caccaaagcg gagaagcacg
     1981 ttcataactt catgatggac actcagctca ccaagcggat caagaatgct gcagccaatg
     2041 tccttcggga aacatggtta atctataaac acacaaagct gctaaagaag attgaccatg
     2101 ccaaagtgag gaaacaccag aggaagttcc tccaagctat ccaccagttg aggagcgtca
     2161 agatggaaca gaggaagctg agtgaccaag ccaacactct ggtggacctt tccaagatgc
     2221 agaatgtcat gtatgactta atcacagaac tcaatgaccg gagcgaagac ctggagaagc
     2281 agattggcag cctggagtcg aagctggagc atctcaccgc cagcttcaac tccctgccgc
     2341 tgctcatcgc cgacaccctg cgccagcagc agcagcagct cctgtctgcc atcatcgagg
     2401 cccggggtgt cagcgtggca gtgggcacca cccacacccc aatctccgat agccccattg
     2461 gggtcagctc cacctccttc ccgaccccgt acacaagttc aagcagttgc taaataaatc
     2521 tccccactcc agaagcatta cccataggtc ttaagatgca aatcaactct ctcctggtcg
     2581 ctttgccatc aagaaacatt cagaccaggg aacggaaaga agagagaccg agctaattaa
     2641 ctaactcatg ttcattcagc gtgcttggtc cgacatgcct tgaaaccaga aatctaatct
     2701 ctgtttaggt gcctctactt gggagcggga agaggagatg acaggaagcg acgcctctgg
     2761 cagggccctt gctgcagagt tggtggagaa cagaaatcca cgctcaatct caggtcttca
     2821 cgcggggggt gggggtcaga tgcactgaag tagccaacag cgaagccagt ccagaagagg
     2881 ggtccgctgg gagggagggt tgtgtcaggc ttgggggatg ggctcttcgc catgggggtc
     2941 tttgaacaca cctctctcct ttccttttgt ctacggaagc ctctgggtga caaaagtaaa
     3001 agagagctgc ccacaacttg ccaaaacaga tatactcgaa tcagactgaa aaaaaaaaaa
     3061 aaagacacag acaaataaaa agccagattt tccactcgat attaataccc acataaacct
     3121 gtgtgtttgc aaacgtgtac atgtacacac atacacatcc cacgttcgct tcaggtcctt
     3181 tcttatttga gcttaatcca aataaaaagg gacttgacac cttaccctgc atacaatagg
     3241 caccccttac atgtgttttg agttggtctg aatctgaaca tggggtgctt tcagttcagg
     3301 tagttagcta gttctggcca cattctgagt ttcactgaag atgtggatcc cttcagacat
     3361 aatgcacatt gctttgtcct ggatatgcac cttgcttgat ttgaaatgga tgccaagcca
     3421 aaattgttgg cattcaggag ggataagcag gcttctaaaa ataacagcat ctgcagagtt
     3481 ttcttcttcc atccaaacaa gttgtgtttc gatggtccac atgaccaggt gtatgtctgt
     3541 aagtgtggag ggagaggaca agaaattgtg catgtgtgtg cagacatgca caaacaggag
     3601 caatccaata acaccttagt gaatagaaat atggttgggg atttgctgag ctgtatttat
     3661 ccagcaacag gtttccagcc ccagatgtta gtagtgcaaa agggccaagt gcctcaattg
     3721 tgagcctctg agctaggagg agaagtgatg aagagtggcc tatgtggtcc cttctacctg
     3781 accttaagtc atctcaaaat gaaatattgt gagaatgaag ggaaccctta gggaaccttg
     3841 tgggtaaggt aagtggacat ggatttgtca gtagcctgtt cctactgtgc catgttaatc
     3901 ttggtgcgaa aaactattcc aatacatctc aaactccaag agacttcaga aacatcaaga
     3961 tttagtgtaa tgagcggcgc agaaaaatgt tttcattgct ccataatctg accacacgta
     4021 acatttgtga cgtgaaaacc atgactttct cattctgagg tctttggttt ctgcctgtgg
     4081 gaaattgaat ggcactgtat ggactatttc atctgttgat ggtaaaacaa aagggtgatt
     4141 tttttgcttg tggttgtcat cttgggttta cctttgtaag aagacatcat caccattctg
     4201 aaggcagtgg cttggcatgg agatttttat tctgtagcac tgggcctgtt cttctaagga
     4261 cagcacaagg tagacaattg tagagccaag gccacttttt caggaagatc tagtctcttg
     4321 ctaaccctct tctttctctc ttgctattgc tgctgctctt ttgatggttt atagtcttaa
     4381 tggcctgcct tgataattcc tttgaacact ttcattagtt gttttgcata ccagagatgc
     4441 cgcacctgct gttggcttat ttttttactt gttctattaa ctgttgatta tctgaatgtt
     4501 tcccctcctc cttgtttcat gtcccaagaa tggtgctcct gttttcagtg acagttcagc
     4561 tgaacatata agcagatgtg taaacagatg aagtaaccat gcagtttcct tgtggcatta
     4621 gttccatttc acaaagtgaa gaccactggt gggctgatct gagtgtgcca atcctgatca
     4681 tttaaaccta cagccttcaa ctggtgattc ctacccaccg ttttcattct gctataaccc
     4741 tgtgatatgt gtgcgtgtgg gtgtgagtgt gggtgtgtgc acatatagag atttagtctt
     4801 aatttcaacc aaatgacatg caagattcca ctgccactct tcctggccaa aagtgtgcac
     4861 agactgtgat ttattcattg tggtctgtga ctttaaccca tcattgatgc tctcacttag
     4921 gtaaacccta aagaccaaac tagcaacact agtcaaggga gtgactggag ttatttctgg
     4981 tagcagtagc cactggcatc ctagaaacac atggacattt gtagcatgaa ttgacctatt
     5041 ggtagtgcaa tagctataca tgatttttat tcttggcaaa agaaaatgct tcaaaaaaaa
     5101 agtgatcaaa cctgcacatt gatcctgtaa tagcaaatgg aaggctattt ctctgtacta
     5161 gcatttcagc tttatgtggg aaagttaccc gttctcctgc aagtacaatc aacccttgat
     5221 gacttaagta ttaattattc tgggtgtagc tcacccaagt tttcttccta catcttttgg
     5281 ctaattccac cacacctcag catcacagtc agatgggaaa aggggcaggt ggattctcat
     5341 gtcatgcctt cttgtacctt attttcaagt tttgtggtgg aggaggttta attatctgct
     5401 caagaatctg gtatatatag ccaggtgcgg tggctcatgc ctgtaatccc aggactttgg
     5461 gcaaggcgag aggatcacct gaggtcagga gttcaagacc agcctggcca acatggcaaa
     5521 accccatctc tactaaaaat acaaaaatta gctgggtttg gtggtggaca cctgtagtcc
     5581 cagctacttg ggaggctgcg gcaggagaat cacttgaacc ccggaagtgg aggttgcagt
     5641 gagccaagat cgtgccattg cactccagct tgggtgaccg agcaagactc cgtctcaaaa
     5701 aaaaaaaaaa aatcggctat atgtaaaaac tattaattat gtaacctcac tccaatactg
     5761 tactgatcaa gatctgccca ctcctagagg actgacccaa tggcagatcc cctactataa
     5821 tctttctgac aactcagtga caggtaaaat cactctaata ttcctaaaat atctagacac
     5881 ttgaattaga aatacagaac cacccccacc cctgccattg cttaagccat attaaagctt
     5941 catgtactgt aaagtcaggg atgtactgtg atactttaag accactaatc tcagtggaat
     6001 ttcaggacat agcattagtt ggtaaattac accagactag gggctcactc ctccttagat
     6061 tgaagtccaa ataaaagtct tcacctttgg atcaaaggcc acatatatat gatcatcatc
     6121 aataaattgt cataaagtgt ctatgaactg ctcttgccat cttcctaata cgtaacttca
     6181 tgacatcatg tctctagaag tgcttaatac aatccaaaag aacaaggggg gaaggcccca
     6241 agtcaccact gaccgacctc agcaggggcc agtggtagaa caacaatctt gtgaattagg
     6301 agacatagaa aatgtacgca aaagtaaatt ccaagcgctg agcatgtaca agctattttt
     6361 attattaaaa gccaagaagg aagagcattg aagaagtaat gattttaata tagtggggga
     6421 tttccccctt aaatttttat aatttccttg tgaataaaca atcatggaaa agaagaaacc
     6481 tagtcataca aaatcagaat aaataaaaat cttaagactg gatccacatc tttggccaat
     6541 tcatataagc acctaataca tcaatttctc tgtacttaaa atgaagagaa taattaatat
     6601 tcttaagtac aatgttttca atagagaaca aatgaaatca cctttgtcat caacagcagc
     6661 ccagactgac tgtatctaga tcacaccttg atgttacgtt tgaatgcagt cagtagttac
     6721 caaaaagtaa ttagtgcaca ttaaatagtg gtaaaatcag cagggagaaa tatgggatta
     6781 gcacgtagtt ccagaacctt cctcctcctc cagagtgcaa ctcaacaaac agtacactcc
     6841 aaattaagaa tcaatgtttc atcccaggac aaacggtgtt gctcccatca cagtaccaca
     6901 actgagatac tgtgaagtca ttgcactacc agtaggttac tactcttttg aagttactgt
     6961 ttttaaatcc taaaatatgt ccccagtttt ctacattcat tcttcttccc ctaagagttg
     7021 ctagaagcag atgaaggatc cctattgact ctgcagggtt tgatttcagt accttagaca
     7081 gctatctggt gacttcccag taggcacgaa caaccccttc tctccccttg cagtcggtgg
     7141 gcttgatgac gccccagaca gtatcctttc cttctctact gccgccaaaa taaacattag
     7201 ggaaatggga tcattaacac cactgagaaa ggagtttaca gggatcgcaa tagtaactgt
     7261 ctgcttgtgg tagtagaaag cttgtgccag gctgcatgaa tagaaattaa tgttaaactc
     7321 ctgttagacg agtaatatgt agatttttat tgaaacagac cacagcttat taaaaagtac
     7381 tcagtgcaat ggtatataca tatatacaga tagaaaaaaa tataactgtg atctttttgt
     7441 cttacgccat tactggaaac acccaaacgt gaactggagt tccctctgaa caaaaccatt
     7501 gtcaaatctt caacatatga cctccaaatc taggtgccaa gtgccctgtg acagttgttt
     7561 acacacctga aaaatgtatc ctggaagctg taatcttggg cctgggctat gtgttgaaac
     7621 aagcaaaaga gacacatcgt gaccaaccaa gctggtggag gaaaagcaca aaattcaact
     7681 ccaacctgca gttaggatcc caaagagatg ttgacattta tagccaaaaa gaaaaagtac
     7741 atatatatat atatatatat atatatatat atatatatat atatatatct gaatcagatc
     7801 gaagcagcca acgtccagct tcactcaaga aggacaagga ataaacaaca ttctctagct
     7861 gtgtcatgtt gtctcagttt ttgaatctca accaggactg atccaccttc atcagatacg
     7921 ttttttaatg gcgcaacaac tcaattttgg tcccagactc tccattctgc actggccacg
     7981 agaacaacaa gagaatgaca ggatgttaat ggaggaaaca ttttcatggc aagctcagaa
     8041 actgaagtgt ttatattcag aatcagatga gctttaatat gaatttgcat cttgcctctg
     8101 tactgcttgc tattttttaa tgataggaga aagcataaca gtttatttta aatatgaaat
     8161 atattgatat attataaaat atattgtaac tttcaattaa cattttgtat caaactagtg
     8221 tttagcaggt ccactgtcag cctgctctcc caatgactgt ttctgagtac acatcactgt
     8281 tagtgattgc cgggcaccca gaaaaagggt cctcctacca cagcctactg aactgccata
     8341 ttctaattct ccaaggcctg ggtattagag acaaaagaac attattccaa gagtagagga
     8401 ataattctac cctaaattct atcaagccat ttcagaagag attttaaaag ccatcactcc
     8461 tttagtagct ctgcaaagcc agtttaataa aagttcaggc tctttctata taaattcaaa
     8521 ggcctcatta cataaattat ataagtctca aacctagcca accagttttc ctttgacaat
     8581 gtcagaattg gacataagaa agagtcctgt tttcctgtaa cttaaaatac tcaaaatcct
     8641 aggcattctg agtttctcca tgtacgtgaa accggggtcg gctttccaaa ctacacaagg
     8701 cccaacaaga gtccagtgtg cagatgtgct tttctaaatt ggcaataccc ttgagggagc
     8761 aaggttccca catgtttatc aaggacatgg agcccagctc ctaagggagt gatgggagga
     8821 gccggcagga catgaacagc cagagccagc aaagagccgg gctgcctttt ctgctagctt
     8881 cctctagctg ggaggaaata aagggttccc gttgagatat tgtcctttat agcaaaatta
     8941 agcaactcat ggaatctaca cccctctttc tctacagagc agctgagaca tacatgtaga
     9001 agttctcagt tccctccttc atatggctaa ggcactactt ctagatgaaa acaggatgtg
     9061 ttcggttaca gaataaagat tttctagaat cagtgagcct cttctcacct gggaccctca
     9121 cttgctgttg ttgctttggt gcaatattgt acaggtgcaa accaggttgc atcggggcag
     9181 attaactgag cctatgtgtc tcttcccttc ccaagccctg aaaattcaca tagatttaca
     9241 gatagtgcaa aacaacaagt tttctcctgg gaaaaggctg gaatccttca aatttcaatt
     9301 tatatgagct taaaggtata taagtcatgg ggctcactga cattaggtat tcatgctgtt
     9361 tgaaagagaa atgtctaaat gcccgttttc agtctgtttg tgtagctgag ccctaacttg
     9421 tacacaagtt ttcttgttat ggagctaaat tctctcagtg cctgtattac tcctcagtta
     9481 tatgttaggc cgagctccac taaagccatc tgaactccag ggcacttgtt tttatcaggt
     9541 tacttactca ccggataatg gaaattggct tattttcaat tttacaattg actgatgaat
     9601 gtattggtgg taattgattt atttcttcac ttttttttgc tactaaatga ttttttcttt
     9661 tttaaaatta ttattattat tattttgaga tggagtctca ctctgtcgcc caggctggag
     9721 tgcagtggcg ccatctcagc tcactgcaag ctctgcctcc tgggttcatg caattctcct
     9781 gcctcagcct cccgagtagc tgggattaca ggtgcgtgcc accacgacca gctaattttt
     9841 ttgtattttt agtagagaca gggtttcatc atgttaacca ggatggtctc gatctcctga
     9901 cctcgtgatc cacctgcctg ggcctcccaa agtgagccac tgtgcctggc ccagtaagtg
     9961 cttttttctt ttggtttttc cttctaacac tgctcagacc aagacttaca tttataaaga
    10021 gcaaatatat tctgaccttt cccctcaaag ttgtattcca tctagaatca aggaaacaaa
    10081 tgcaaattca tcatttgggg aaagcagacc tcttgacagt aattcaagtc catggctata
    10141 tgctaaccct tgctactcaa agtgtggtcc acagaccagc agcgttagca tcatctgtaa
    10201 gcttgttaga aatgcagaat ctcaggcccc acatcaaacc ttcttcatca gaattggcct
    10261 tttaacaaat ccccaggtga cctgtatgca ccagaaatat tgataagcac tggcctcacc
    10321 tcaccgtagt gataactaac ctcgttaata attagagaat gaagctatct cttccttgag
    10381 tctcatttac agaattattt gaagatgtga aggtcctgaa tgatcataag gattaaaaaa
    10441 atgcatcttt aaattccact tgaggttata taacttggat ggtctacatc aaacataata
    10501 aagtcaataa gagatagtct ttttccggtc ttacctaaat agcactccat atccttgagc
    10561 cacctcaaag cccccatttt taagttttag tctttttttt tttagaccag actagcaaag
    10621 ccatcagaat atcaggtgga acctcagggc aggtcctctg gaaggctaca ggagtctctg
    10681 cttagcatag tacagaaatt gttgtgtttc tcgatccttg tgttcttccc ttgaggaact
    10741 cagcatctca ccagtaagaa ctcccctccc ctagaggaaa atagcaagag aagttagttt
    10801 aaccatctag aaacattgct aatctccttg aaagccaatt ctcttactca ttgcaccaac
    10861 aagaaacact ccaaggtggg caaggaaaga cgtgaattct gatattggcg ccataactcg
    10921 atatgtaccc agaaacagtt atgttctaaa ttaatggctt taacctgttc attgtaaaaa
    10981 gtgccttgga cttcaatcta aagaggtcag tataaatatg tatgtgtgtg tgtgacgtgt
    11041 atgtaggcaa atatttacag atttatacat acataggtgc acacacatat acccgcacat
    11101 tcatatataa tatatacata catatataaa tgcttcataa tatatcatat tgctattcac
    11161 agctccattt cttttgtctg gtcctatgtt tatttgttta gtgagacctt tacatagaga
    11221 ggttctattc ggaacattga tgtatttttt gtttgttttt tactttttat taaaaaggta
    11281 aaggatatta aaaaaaaaaa aaaaagtcaa gtgcctgaag gttttgaatg gagttacggt
    11341 aaactttctc aggtcaccaa agaggaaaca tttttccttt ggaaaacatt tttctgtttc
    11401 agtgactttg ttttcccttg gtggtaatgc ataaataaga gtggttaaag tgattttgta
    11461 agttttattt caatccattt ctgtggttcc cataaggcaa tagctaaaaa tttgttcagt
    11521 ctagtgccca gttttctcca cacatcagca tcagaagggc ccggtcttca catgctctgt
    11581 gtggttttgt tcgcgttgtt actgtgttcc ttactaagga aaggcaaatg aaattgcagg
    11641 ggctggagct tagaccagct ccaaatatgg cttccttttg gtaacacaca cagaaggtgg
    11701 actagtggga agtctctctc cctgaagcag gtgggaacct gtgtcttaga aggcagagga
    11761 gtcccagtga actctccaca gctccatggg ccctgcctgt ctacagttat acaactgcct
    11821 gtatgagtca gcctttctct accttagcca aaggtcttct ctttctgtgg tgtgacagct
    11881 tgtaatgaac caacttgctc tggttagaag tccctagagc tccaggaaga gtcgccaggt
    11941 ttccattgct gtgctcaaag ctcaaggaca cattatactt ctttaaacca actaaatctc
    12001 tctagctccc tgcccccatg gtgacacttt cataataggc agggccaagt cagagaggtc
    12061 atgccctgta tacagtgtca atcagaagag acactgcaaa atgtcagagg tgaccagaaa
    12121 gacaacagat ctctccagct gtcctcacag ctgcaggcac atttgtggaa ttgtgagtag
    12181 gaaggcttgg cagccagggg atgggaaatg aatttcccca agttgaaaac cctgtaccct
    12241 tactttcctt cccataaatt tttctgtagt tctaggtaaa taataataat aataaaaatg
    12301 caaattaggt gctttagaag gagtatgtaa tatctgggac tttttctaag ttggtagacc
    12361 taaaaaatgt tttcaaaaat atatctagct gcatttctac tgctgtcatt ccttaaagct
    12421 cttcctccaa aaactccata tgaatgaata catttaccaa ctcagtgatt actaaataat
    12481 agtactttat acttatacac agtaatacct ttcatctaag gatctcaaat gccaatatat
    12541 tagtcatcac cctgtaaggt ggatgacata ttattcccat tattccaatg ggaaaattgg
    12601 gccatagaaa actgaggagc aaatgactca tctacaggaa ttaaatggaa aaaacaggct
    12661 aggatttctc agcacacttt aggagtgaat gaaaacttac aggcttcagt tctactgctg
    12721 gccaccattg gatttgtaag atccaggatg tgtattgacc acatgtgtcc agacccaggc
    12781 ttagggcatc tggaatgaga gtggtgggct ggtgtgtggg tctgaggatc tggatgggag
    12841 actgcatttt cttctctgtg caaaatatgg aagtgtgacc ttgaaggtgg gcttagtcta
    12901 tggccttccc cactcctgct tgaactgaag ctggagagaa tgggcatttt taaatgttac
    12961 ggcatatgct aatataatat tatggcatta aataaaaaca agaagagaac tgactaaaac
    13021 caaaaaacca aaaa
//
