LOCUS       NM_014505               4717 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily M
            regulatory beta subunit 4 (KCNMB4), mRNA.
ACCESSION   NM_014505
VERSION     NM_014505.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Iwanicki,T., Balcerzyk,A., Kazek,B., Emich-Widera,E., Likus,W.,
            Iwanicka,J., Kapinos-Gorczyca,A., Kapinos,M., Jarosz,A.,
            Grzeszczak,W., Gorczynska-Kosiorz,S. and Niemiec,P.
  TITLE     Family-Based Cohort Association Study of PRKCB1, CBLN1 and KCNMB4
            Gene Polymorphisms and Autism in Polish Population
  JOURNAL   J Autism Dev Disord 52 (10), 4213-4218 (2022)
   PUBMED   34562210
  REMARK    GeneRIF: Family-Based Cohort Association Study of PRKCB1, CBLN1 and
            KCNMB4 Gene Polymorphisms and Autism in Polish Population.
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4717)
  AUTHORS   Tao,X. and MacKinnon,R.
  TITLE     Molecular structures of the human Slo1 K+ channel in complex with
            beta4
  JOURNAL   Elife 8, e51409 (2019)
   PUBMED   31815672
  REMARK    GeneRIF: This study presents cryo-EM structures of Slo1 in complex
            with the auxiliary protein, beta4. Four beta4, each containing two
            transmembrane helices, encircle Slo1, contacting it through helical
            interactions inside the membrane.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4717)
  AUTHORS   Velazquez-Marrero,C., Seale,G.E., Treistman,S.N. and Martin,G.E.
  TITLE     Large conductance voltage- and Ca2+-gated potassium (BK) channel
            beta4 subunit influences sensitivity and tolerance to alcohol by
            altering its response to kinases
  JOURNAL   J Biol Chem 289 (42), 29261-29272 (2014)
   PUBMED   25190810
  REMARK    GeneRIF: BK channel beta4 subunit influences sensitivity and
            tolerance to alcohol by altering its response to kinases
REFERENCE   5  (bases 1 to 4717)
  AUTHORS   Yang,J., Annala,M., Ji,P., Wang,G., Zheng,H., Codgell,D., Du,X.,
            Fang,Z., Sun,B., Nykter,M., Chen,K. and Zhang,W.
  TITLE     Recurrent LRP1-SNRNP25 and KCNMB4-CCND3 fusion genes promote tumor
            cell motility in human osteosarcoma
  JOURNAL   J Hematol Oncol 7, 76 (2014)
   PUBMED   25300797
  REMARK    GeneRIF: Two recurrent fusion genes associated with the 12q locus,
            LRP1-SNRNP25 and KCNMB4-CCND3, were by RT-PCR, Sanger sequencing
            and FISH, and were found to be osteosarcoma specific in a
            validation cohort of 240 other sarcomas.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4717)
  AUTHORS   Jin,P., Weiger,T.M., Wu,Y. and Levitan,I.B.
  TITLE     Phosphorylation-dependent functional coupling of hSlo
            calcium-dependent potassium channel and its hbeta 4 subunit
  JOURNAL   J Biol Chem 277 (12), 10014-10020 (2002)
   PUBMED   11790768
REFERENCE   7  (bases 1 to 4717)
  AUTHORS   Behrens,R., Nolting,A., Reimann,F., Schwarz,M., Waldschutz,R. and
            Pongs,O.
  TITLE     hKCNMB3 and hKCNMB4, cloning and characterization of two members of
            the large-conductance calcium-activated potassium channel beta
            subunit family
  JOURNAL   FEBS Lett 474 (1), 99-106 (2000)
   PUBMED   10828459
REFERENCE   8  (bases 1 to 4717)
  AUTHORS   Weiger,T.M., Holmqvist,M.H., Levitan,I.B., Clark,F.T., Sprague,S.,
            Huang,W.J., Ge,P., Wang,C., Lawson,D., Jurman,M.E.,
            Glucksmann,M.A., Silos-Santiago,I., DiStefano,P.S. and Curtis,R.
  TITLE     A novel nervous system beta subunit that downregulates human large
            conductance calcium-dependent potassium channels
  JOURNAL   J Neurosci 20 (10), 3563-3570 (2000)
   PUBMED   10804197
REFERENCE   9  (bases 1 to 4717)
  AUTHORS   Meera,P., Wallner,M. and Toro,L.
  TITLE     A neuronal beta subunit (KCNMB4) makes the large conductance,
            voltage- and Ca2+-activated K+ channel resistant to charybdotoxin
            and iberiotoxin
  JOURNAL   Proc Natl Acad Sci U S A 97 (10), 5562-5567 (2000)
   PUBMED   10792058
REFERENCE   10 (bases 1 to 4717)
  AUTHORS   Brenner,R., Jegla,T.J., Wickenden,A., Liu,Y. and Aldrich,R.W.
  TITLE     Cloning and functional characterization of novel large conductance
            calcium-activated potassium channel beta subunits, hKCNMB3 and
            hKCNMB4
  JOURNAL   J Biol Chem 275 (9), 6453-6461 (2000)
   PUBMED   10692449
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025569.37 and BC042446.2.
            
            On Nov 22, 2018 this sequence version replaced NM_014505.5.
            
            Summary: MaxiK channels are large conductance, voltage and
            calcium-sensitive potassium channels which are fundamental to the
            control of smooth muscle tone and neuronal excitability. MaxiK
            channels can be formed by 2 subunits: the pore-forming alpha
            subunit and the modulatory beta subunit. The protein encoded by
            this gene is an auxiliary beta subunit which slows activation
            kinetics, leads to steeper calcium sensitivity, and shifts the
            voltage range of current activation to more negative potentials
            than does the beta 1 subunit. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.193257.1,
                                           SRR1660803.206977.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258111.5/ ENSP00000258111.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-283               AC025569.37        174499-174781       c
            284-1444            BC042446.2         1-1161
            1445-4717           AC025569.37        106779-110051       c
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q15"
     gene            1..4717
                     /gene="KCNMB4"
                     /note="potassium calcium-activated channel subfamily M
                     regulatory beta subunit 4"
                     /db_xref="GeneID:27345"
                     /db_xref="HGNC:HGNC:6289"
                     /db_xref="MIM:605223"
     exon            1..781
                     /gene="KCNMB4"
                     /inference="alignment:Splign:2.1.0"
     CDS             446..1078
                     /gene="KCNMB4"
                     /note="large conductance calcium-dependent potassium ion
                     channel beta 4 subunit; hbeta4; BKbeta4; slo-beta-4;
                     k(VCA)beta-4; BK channel subunit beta-4; maxi K channel
                     subunit beta-4; charybdotoxin receptor subunit beta-4;
                     calcium-activated potassium channel, subfamily M subunit
                     beta-4; potassium large conductance calcium-activated
                     channel, subfamily M, beta member 4; BK channel beta
                     subunit 4; MaxiK channel beta-subunit 4; big potassium
                     channel beta subunit 4; potassium channel subfamily M
                     regulatory beta subunit 4"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     beta-4"
                     /protein_id="NP_055320.4"
                     /db_xref="CCDS:CCDS8997.1"
                     /db_xref="GeneID:27345"
                     /db_xref="HGNC:HGNC:6289"
                     /db_xref="MIM:605223"
                     /translation="MAKLRVAYEYTEAEDKSIRLGLFLIISGVVSLFIFGFCWLSPAL
                     QDLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHS
                     DEHQLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVL
                     LHRTHDEIVLLHCFLWPLVTFVVGVLIVVLTICAKSLAVKAEAMKKRKFS"
     exon            782..909
                     /gene="KCNMB4"
                     /inference="alignment:Splign:2.1.0"
     exon            910..4717
                     /gene="KCNMB4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1421..1426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMB4"
                     /note="hexamer: ATTAAA"
     polyA_site      1444
                     /gene="KCNMB4"
                     /note="major polyA site"
     regulatory      1605..1610
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMB4"
                     /note="hexamer: ATTAAA"
     polyA_site      1638
                     /gene="KCNMB4"
     regulatory      4698..4703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMB4"
                     /note="hexamer: AATAAA"
     polyA_site      4717
                     /gene="KCNMB4"
                     /note="major polyA site"
ORIGIN      
        1 agacactcct agccttgggg cagctgccgg gcgagtcagc ggagtagcgg ccagcgggcg
       61 atggagacag agagacaccc gacgagagga ggcggggtgg gggaggcggg gagagtgcgg
      121 gggcggaggc tggcaggggg cgctggaagc tggagcggtc cgtgcgctcc ccgcgcccga
      181 gggtgcagga ggctctgaag cggctgctgc accgcggggc ccaggcggcg gctggggggc
      241 tggggggcgc tgccgccgcc gccgccgggg gcgtcgctgg cctcggcccc tttgttctcg
      301 cgcgctcccc ctcgccgccc actcccctgc tgtcgcgcgg cggcggcggt ggcggcggcg
      361 gctcctcccg cccgaggcag tcgggctcgg cgccgggggc gggagggggc ggggggagca
      421 cgccagccgc cgagagtggg gggcgatggc gaagctccgg gtggcttacg agtacacgga
      481 agccgaggac aagagcatcc ggctcggctt gtttctcatc atctccggcg tcgtgtcgct
      541 cttcatcttc ggcttctgct ggctgagtcc cgcgctgcag gatctgcaag ccacggaggc
      601 caattgcacg gtgctgtcgg tgcagcagat cggcgaggtg ttcgagtgca ccttcacctg
      661 tggcgccgac tgcaggggca cctcgcagta cccctgcgtc caggtctacg tgaacaactc
      721 tgagtccaac tctagggcgc tgctgcacag cgacgagcac cagctcctga ccaaccccaa
      781 gtgctcctat atccctccct gtaagagaga aaatcagaag aatttggaaa gtgtcatgaa
      841 ttggcaacag tactggaaag atgagattgg ttcccagcca tttacttgct attttaatca
      901 acatcaaaga ccagatgatg tgcttctgca tcgcactcat gatgagattg tcctcctgca
      961 ttgcttcctc tggcccctgg tgacatttgt ggtgggcgtt ctcattgtgg tcctgaccat
     1021 ctgtgccaag agcttggcgg tcaaggcgga agccatgaag aagcgcaagt tctcttaaag
     1081 gggaaggagg cttgtagaaa gcaaagtaca gaagctgtac tcatcggcac gcgtccacct
     1141 gcggaacctg tgtttcctgg cgcaggagat ggacagggcc acgacagggc tctgagaggc
     1201 tcatccctca gtggcaacag aaacaggcac aactggaaga cttggaacct caaagcttgt
     1261 attccatctg ctgtagcaat ggctaaaggg tcaagatctt agctgtatgg agtaactatt
     1321 tcagaaaacc ctataagaag ttcattttct ttcaaaagta acagtatatt atttgtacag
     1381 tgtagtatac aaaccattat gatttatgct acttaaaaat attaaaatag agtggtctgt
     1441 gttattttct atttcctttt ttatgcttag aacaccaggg tttaaaaaaa aaaaaaaggt
     1501 gaggacatct gggtctcatt tgcttctgct aggttaaact tttacttgac aacaaggatt
     1561 cctgctgaag tctgaacctt actgtgtaac cctcagtttc cactattaaa gagtatcttt
     1621 tgacgtctgc ttggaaaatg aatagtatac tggtaactca gtctccagtc acctctgtgt
     1681 ctcttaagca agagattcta aaagattggg aaaacatatc ctccaacacc tgcctttgcc
     1741 taaccattat ttttcaccag attacttctt aagagaggga ggtgattctg aagaaggctt
     1801 ctatctcaaa aagcactggg cttccttatt catctgttct tgttgttttt gacggagtta
     1861 aaaaagtttg tgtgcaatac aatatacatg atgtgaagga cactcttcag cttagtgaaa
     1921 cgctgttttc attttttttt ttttttgtag gtcagaaaaa aacaacaaaa tcagttcaag
     1981 catttttttt tctttgtcct tgccttgatg ttatgagtat taaaaccagg aggattgctg
     2041 ccattgtgca gtttgcttag acaaacctgg agatgcaacc cagctcacat cattgctact
     2101 gatgagcttt ctgtgccttt atcaaaagtt gattgagaag accatatttc tttgtatctt
     2161 tttataaact caaattccaa gtatcaaatc gcaggtctca gtgaacatca aacctattta
     2221 ctacatagaa tcaaaccttt gtttaggtga gatgtacatc gttagtggag gaaaaactga
     2281 caacctaatt tcatttgttt tcttctgata ctcttcagac atgcctctat tagaataaag
     2341 gtaaactgga atttaaagac aagttcccct cagttatttc catggagctg taatatgtat
     2401 atatggagtg atggtttcct gacctttagt ccacatacca atgttttctt ttttcttttt
     2461 tttttttttt tttgagatgg tgtctcactc tgttgccagg ctggagtgca gtggcacgat
     2521 ctcggctcac tacagtctcc acctcctggg ttcaagtcat tcctctgcct cagcctcccg
     2581 agtagctggg actacaggca cgcaccacca cgcctggcta atttttttgt atttttagta
     2641 gagacggggt ttcaccgtgt tagccaggat ggtctcaatc tcctgacctt gtgatctgcc
     2701 cacttcacct cccaaagtgc tgggattaca ggccaatgtt ttcttaatct tagaatgtga
     2761 ataactgaaa atcatagtct gtggaaaggt gttgaattga gtataatctt cttctgttta
     2821 tttttgtgtt ttgtttttta acagatgggt atcttgctat gttgcccagg atggagtgca
     2881 gtagctattc acaggtatga tcatagcaca ctgcagcctc aagctcctgg gctcaagcga
     2941 tccccctccc tcagcctccc aagtatctgg ggttactggt gtgcaccacc gtgcttggct
     3001 ccaataattt tttttctaat tcaaaagtta cagtttcact gtgaaaaagg ccttgaacac
     3061 actatttatg acatcttttg aggcagctcc agtgccttga cttcaatccc agtttccggt
     3121 tgcagcatcc ttgttgtctt agcaacacag tgaactattc tgaagcatag agtaacacga
     3181 aactgggagt ccgagaaata atcatctctg catcacatta tgggagacga agtctgcttt
     3241 atccatttta tctttattca gttgtctatg attaattgat tacagagtag tagattagaa
     3301 tagtgcatgg atatacattt gtgttgaaaa aaggggaagt tgatatatat caatcttagt
     3361 tttcatttat cagtttgata ttcatgcatt tacactaaac gcttccattt atcccgaaaa
     3421 agtatatgca actgtattct gtaggttgat ttttggaaaa ggggagaagc acactgaatt
     3481 cataaggtca catgtagtct taaggtctta cttgcttaca gccaattaaa tttgaagcac
     3541 cttatttata cttgttaaag gtaaaaccca aaagaacaag cagaggacat tttaaggtca
     3601 taaaaggtaa ataagcttac cttcttaatg ttttcattct ctttttgtat aaatcagaaa
     3661 atgatctaaa ctgctgtaac aaagagaccc caaaatatga tggctcatgt aagataattt
     3721 atttttttct cacatagcaa tccagaagtg gcttcatttc acaaggtatt caagggatat
     3781 aggagtcatc taccttgtta gttctcttaa tacccaaggg tattgttctt tccatggtca
     3841 aagctggctc aagacttcct agcctgtgaa aaaagaagaa ggtggagcaa gccatttcct
     3901 ttttaggaaa ttacagccat cacttctgcc caccgtccat tcatgaatac ttactatata
     3961 gctataccta gcttcaagaa agcctgggac gtgtctctaa ctagatggac atgtgcccta
     4021 ctaaaactcc agggaaaggg ttctattact aaagctaaaa agaggggaat gaatactaga
     4081 gttaaagaca aaaatgatag cagccaatgg cccatgccgt gataatctgc tgagcaggca
     4141 tgatggagat cccttgccca gcagaaagtg ttccttggtg aaatcatgaa tctgctatct
     4201 aggagaaact cccttgtcca ttgtcttctg tggccactag tttgacctct aggaaagtct
     4261 tgctcgtcag cttctgtggc cccgtctgaa acttttgagg gacatcgcag cttttgcagc
     4321 ccctgcttgc tggtgcagac ttttagacct agattgcctt agagactgaa aaatatacgc
     4381 ttttataggc cggggtttta gttcatttga ctgtaataaa gacgtcaatg ccgtttttaa
     4441 tgtttgactg ctgacatctt tcaagactca cctttccctt ctcccttatg ctgcacatct
     4501 gggcaagctg atggaagcat gggtgcctcc tcctttggcc ccagcaggaa gttcaaatca
     4561 cgcaagccct ggcatgcatg caggaagctt caccccagcc tcacactcta agacggataa
     4621 aagccaaacc aattaagccg tttctcgacc ctcctgggag cctgccctat ctccctggaa
     4681 agtctcagta tgtgagtaat aaaccttttt ataccca
//
