LOCUS       NM_181361               2543 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily M
            regulatory beta subunit 2 (KCNMB2), transcript variant 1, mRNA.
ACCESSION   NM_181361
VERSION     NM_181361.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Tian,Y., Ullrich,F., Xu,R., Heinemann,S.H., Hou,S. and Hoshi,T.
  TITLE     Two distinct effects of PIP2 underlie auxiliary subunit-dependent
            modulation of Slo1 BK channels
  JOURNAL   J Gen Physiol 145 (4), 331-343 (2015)
   PUBMED   25825171
REFERENCE   3  (bases 1 to 2543)
  AUTHORS   Xie,T., Deng,L., Mei,P., Zhou,Y., Wang,B., Zhang,J., Lin,J.,
            Wei,Y., Zhang,X. and Xu,R.
  TITLE     Genome-wide association study combining pathway analysis for
            typical sporadic amyotrophic lateral sclerosis in Chinese Han
            populations
  JOURNAL   Neurobiol Aging 35 (7), 1778 (2014)
   PUBMED   24529757
REFERENCE   4  (bases 1 to 2543)
  AUTHORS   Manzanares,D., Srinivasan,M., Salathe,S.T., Ivonnet,P., Baumlin,N.,
            Dennis,J.S., Conner,G.E. and Salathe,M.
  TITLE     IFN-gamma-mediated reduction of large-conductance, Ca2+-activated,
            voltage-dependent K+ (BK) channel activity in airway epithelial
            cells leads to mucociliary dysfunction
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 306 (5), L453-L462 (2014)
   PUBMED   24414257
  REMARK    GeneRIF: IFN-gamma changed mRNA levels of the BK beta-modulatory
            proteins KCNMB2 (increased) and KCNMB4 (decreased) as well as
            leucine-rich repeat-containing protein (LRRC)26 (decreased).
REFERENCE   5  (bases 1 to 2543)
  AUTHORS   Sun,X., Shi,J., Delaloye,K., Yang,X., Yang,H., Zhang,G. and Cui,J.
  TITLE     The interface between membrane-spanning and cytosolic domains in
            Ca(2)+-dependent K+ channels is involved in beta subunit modulation
            of gating
  JOURNAL   J Neurosci 33 (27), 11253-11261 (2013)
   PUBMED   23825428
  REMARK    GeneRIF: beta subunits of large-conductance calcium-dependent
            potassium channels modulate the interface between membrane-spanning
            and cytosolic domains.
REFERENCE   6  (bases 1 to 2543)
  AUTHORS   Uebele,V.N., Lagrutta,A., Wade,T., Figueroa,D.J., Liu,Y.,
            McKenna,E., Austin,C.P., Bennett,P.B. and Swanson,R.
  TITLE     Cloning and functional expression of two families of beta-subunits
            of the large conductance calcium-activated K+ channel
  JOURNAL   J Biol Chem 275 (30), 23211-23218 (2000)
   PUBMED   10766764
REFERENCE   7  (bases 1 to 2543)
  AUTHORS   Meera,P., Wallner,M. and Toro,L.
  TITLE     A neuronal beta subunit (KCNMB4) makes the large conductance,
            voltage- and Ca2+-activated K+ channel resistant to charybdotoxin
            and iberiotoxin
  JOURNAL   Proc Natl Acad Sci U S A 97 (10), 5562-5567 (2000)
   PUBMED   10792058
REFERENCE   8  (bases 1 to 2543)
  AUTHORS   Brenner,R., Jegla,T.J., Wickenden,A., Liu,Y. and Aldrich,R.W.
  TITLE     Cloning and functional characterization of novel large conductance
            calcium-activated potassium channel beta subunits, hKCNMB3 and
            hKCNMB4
  JOURNAL   J Biol Chem 275 (9), 6453-6461 (2000)
   PUBMED   10692449
REFERENCE   9  (bases 1 to 2543)
  AUTHORS   Xia,X.M., Ding,J.P. and Lingle,C.J.
  TITLE     Molecular basis for the inactivation of Ca2+- and voltage-dependent
            BK channels in adrenal chromaffin cells and rat insulinoma tumor
            cells
  JOURNAL   J Neurosci 19 (13), 5255-5264 (1999)
   PUBMED   10377337
REFERENCE   10 (bases 1 to 2543)
  AUTHORS   Wallner,M., Meera,P. and Toro,L.
  TITLE     Molecular basis of fast inactivation in voltage and Ca2+-activated
            K+ channels: a transmembrane beta-subunit homolog
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 4137-4142 (1999)
   PUBMED   10097176
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC017825.1, AB193524.1,
            AF209747.1, BF434939.1 and BM983559.1.
            
            On Nov 23, 2018 this sequence version replaced NM_181361.2.
            
            Summary: MaxiK channels are large conductance, voltage and
            calcium-sensitive potassium channels which are fundamental to the
            control of smooth muscle tone and neuronal excitability. MaxiK
            channels can be formed by 2 subunits: the pore-forming alpha
            subunit and the modulatory beta subunit. The protein encoded by
            this gene is an auxiliary beta subunit which decreases the
            activation time of MaxiK alpha subunit currents. Alternative
            splicing results in multiple transcript variants of this gene.
            Additional variants are discussed in the literature, but their full
            length nature has not been described. [provided by RefSeq, Jul
            2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.25118.1,
                                           SRR1660803.218457.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000452583.6/ ENSP00000397483.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-630               BC017825.1         1-630
            631-907             AB193524.1         231-507
            908-2368            AF209747.1         917-2377
            2369-2532           BF434939.1         12-175              c
            2533-2543           BM983559.1         18-28               c
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.32"
     gene            1..2543
                     /gene="KCNMB2"
                     /note="potassium calcium-activated channel subfamily M
                     regulatory beta subunit 2"
                     /db_xref="GeneID:10242"
                     /db_xref="HGNC:HGNC:6286"
                     /db_xref="MIM:605214"
     exon            1..276
                     /gene="KCNMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..399
                     /gene="KCNMB2"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..1051
                     /gene="KCNMB2"
                     /note="MaxiK channel beta 2 subunit; large conductance
                     calcium-activated potassium channel beta 2 subunit;
                     large-conductance Ca2+-activated K+ channel beta2 subunit;
                     hbeta2; slo-beta-2; k(VCA)beta-2; BK channel subunit
                     beta-2; potassium channel subfamily M regulatory beta
                     subunit 2; charybdotoxin receptor subunit beta-2;
                     hCG1646471; potassium large conductance calcium-activated
                     channel, subfamily M, beta member 2; BK channel beta
                     subunit 2; MaxiK channel beta-subunit 2; big potassium
                     channel beta subunit 2"
                     /codon_start=1
                     /product="calcium-activated potassium channel subunit
                     beta-2"
                     /protein_id="NP_852006.1"
                     /db_xref="CCDS:CCDS3223.1"
                     /db_xref="GeneID:10242"
                     /db_xref="HGNC:HGNC:6286"
                     /db_xref="MIM:605214"
                     /translation="MFIWTSGRTSSSYRHDEKRNIYQKIRDHDLLDKRKTVTALKAGE
                     DRAILLGLAMMVCSIMMYFLLGITLLRSYMQSVWTEESQCTLLNASITETFNCSFSCG
                     PDCWKLSQYPCLQVYVNLTSSGEKLLLYHTEETIKINQKCSYIPKCGKNFEESMSLVN
                     VVMENFRKYQHFSCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGGVAIVAMVK
                     LTQYLSLLCERIQRINR"
     exon            400..570
                     /gene="KCNMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            571..766
                     /gene="KCNMB2"
                     /inference="alignment:Splign:2.1.0"
     exon            767..2543
                     /gene="KCNMB2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2480..2485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMB2"
                     /note="hexamer: AATAAA"
     polyA_site      2505
                     /gene="KCNMB2"
                     /note="major polyA site"
     regulatory      2519..2524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMB2"
                     /note="hexamer: ATTAAA"
     polyA_site      2543
                     /gene="KCNMB2"
ORIGIN      
        1 agtatgaagc agctttaacg gagctccaga taactaattt caagcatggc tgtctagcgt
       61 gcctgtcact cctattgtcc ttccaaactc tttcagacat tttgagcagg gagtattaaa
      121 gctatgagtt agaaagggtt gtgacattaa tggtccacaa aggctttagg cacaagaggt
      181 aatgatgatt accggtggct atttggagga ctgcaccgga tcgctgtcat taaaaattaa
      241 gcgtggcttt tgaggaagat gtgacaacta ccggaggtct ttttgccatt cctccaggac
      301 atccaccata aggaaaggag accctggacc aacattctct aagatgttta tatggaccag
      361 tggccggacc tcttcatctt atagacatga tgaaaaaaga aatatttacc agaaaatcag
      421 ggaccatgac ctcctggaca aaaggaaaac agtcacagca ctgaaggcag gagaggaccg
      481 agctattctc ctgggactgg ctatgatggt gtgctccatc atgatgtatt ttctgctggg
      541 aatcacactc ctgcgctcat acatgcagag cgtgtggacc gaagagtctc aatgcacctt
      601 gctgaatgcg tccatcacgg aaacatttaa ttgctccttc agctgtggtc cagactgctg
      661 gaaactttct cagtacccct gcctccaggt gtacgttaac ctgacttctt ccggggaaaa
      721 gctcctcctc taccacacag aagagacaat aaaaatcaat cagaagtgct cctatatacc
      781 taaatgtgga aaaaattttg aagaatccat gtccctggtg aatgttgtca tggaaaactt
      841 caggaagtat caacacttct cctgctattc tgacccagaa ggaaaccaga agagtgttat
      901 cctaacaaaa ctctacagtt ccaacgtgct gttccattca ctcttctggc caacctgtat
      961 gatggctggg ggtgtggcaa ttgttgccat ggtgaaactt acacagtacc tctccctact
     1021 atgtgagagg atccaacgga tcaatagata aatgcaaaaa tggataaaat aatttttgtt
     1081 aaagctcaaa tactgttttc tttcattctt caccaaagaa ccttaagttt gtaacgtgca
     1141 gtctgttatg agttccctaa tatattctta tatgtagagc aataatgcaa aagctgttct
     1201 atatgcaaac atgatgtctt tattattcag gagaataaat aactgttttg tgttggttgg
     1261 tggttttcat aatcttattt ctgtactgga actagtactt tcttctctca ttccgccaaa
     1321 acagggctca gttattcatt tgccaagctt cgtggaggaa tgtaggtgac atcaatgtga
     1381 taaagtctgt gttctgagtt gtcagatctc ttgaagacaa tatttttcat cacttattgt
     1441 ttactaaagc tacagccaaa aatatttttt tttcttattc taaactgagc cctatagcaa
     1501 gtgaagggac cagatttcct aattaaagga agttaggtac ttttcttgta ttttttacca
     1561 tatcactgta aagaagaggg gaaacccagc cagctacttt ttttcatcac tttttattca
     1621 taacttcaga tttgtaaaac taatttccaa aatataagct gttttcatta gccagttcta
     1681 taatatcttc ctgtgattta tgtagaaaat gaacacaccc cttttccatt taagaccctg
     1741 ctactgtgtg aagagatgat acttacaagg agtgtcatta cctgtgagct gactgaatgt
     1801 tggtaggtgc tccattacaa tccaggaaag tctgtgttac tgatatttgt gtggaaatct
     1861 ttatttcact tcaatttaac cattagatgg taaaattaag atgctacttg ttggtaaaaa
     1921 ttggtggact ggtttcaatg ggtaaatgtg ttgtggcaaa ttaatgtgtt ggaatattgc
     1981 tctttgtgaa tttgtgctta agtcaatgaa tgtgtagtat ctccttctga caagcattcc
     2041 ctattgggat tttaaagcta tgtgcacaga atattagtct cttctacatg ttttattttt
     2101 ctatttataa ttcccttttt tgttgttata ttttatacac agaatagatc ttttttctaa
     2161 cacatatttg aactgaataa cagacttaaa gaaagccttt gttcacattg ctatttactt
     2221 ttgtgtttgg gggaaaatac gagggattga ttttaaataa aaaacattcc atctttcatt
     2281 taatatcaat atcaaaagaa gaagacaaac atctatcttt ctcatctata tttaagtacc
     2341 tttttgtaat gtagtatcaa agttttttag gtaatgcaaa attttacaaa tcatttgtgg
     2401 aatgaatggt aaaactaatc tgatgaaatg gaaaattatt ctgcaatatt gtaattcata
     2461 gtttgacttt tcataagcaa ataaatccct aggatgtaat caggacttca aatgtgtaat
     2521 taaatttttt taaaaaaaat cta
//
