LOCUS       NM_001322829           11976 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily M alpha
            1 (KCNMA1), transcript variant 10, mRNA.
ACCESSION   NM_001322829
VERSION     NM_001322829.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Dinsdale,R.L. and Meredith,A.L.
  TITLE     Evaluation of four KCNMA1 channelopathy variants on BK channel
            current under CaV1.2 activation
  JOURNAL   Channels (Austin) 18 (1), 2396346 (2024)
   PUBMED   39217512
  REMARK    GeneRIF: Evaluation of four KCNMA1 channelopathy variants on BK
            channel current under CaV1.2 activation.
REFERENCE   2  (bases 1 to 11976)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   3  (bases 1 to 11976)
  AUTHORS   Moldenhauer,H.J., Tammen,K. and Meredith,A.L.
  TITLE     Structural mapping of patient-associated KCNMA1 gene variants
  JOURNAL   Biophys J 123 (14), 1984-2000 (2024)
   PUBMED   38042986
  REMARK    GeneRIF: Structural mapping of patient-associated KCNMA1 gene
            variants.
REFERENCE   4  (bases 1 to 11976)
  AUTHORS   Dinsdale,R.L., Roache,C.E. and Meredith,A.L.
  TITLE     Disease-associated KCNMA1 variants decrease circadian clock
            robustness in channelopathy mouse models
  JOURNAL   J Gen Physiol 155 (11) (2023)
   PUBMED   37728576
  REMARK    GeneRIF: Disease-associated KCNMA1 variants decrease circadian
            clock robustness in channelopathy mouse models.
REFERENCE   5  (bases 1 to 11976)
  AUTHORS   Ohya,S., Kajikuri,J., Kito,H. and Matsui,M.
  TITLE     Down-Regulation of CYP3A4 by the KCa1.1 Inhibition Is Responsible
            for Overcoming Resistance to Doxorubicin in Cancer Spheroid Models
  JOURNAL   Int J Mol Sci 24 (21), 15672 (2023)
   PUBMED   37958656
  REMARK    GeneRIF: Down-Regulation of CYP3A4 by the KCa1.1 Inhibition Is
            Responsible for Overcoming Resistance to Doxorubicin in Cancer
            Spheroid Models.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11976)
  AUTHORS   McCobb,D.P., Fowler,N.L., Featherstone,T., Lingle,C.J., Saito,M.,
            Krause,J.E. and Salkoff,L.
  TITLE     A human calcium-activated potassium channel gene expressed in
            vascular smooth muscle
  JOURNAL   Am J Physiol 269 (3 Pt 2), H767-H777 (1995)
   PUBMED   7573516
REFERENCE   7  (bases 1 to 11976)
  AUTHORS   Tseng-Crank,J., Foster,C.D., Krause,J.D., Mertz,R., Godinot,N.,
            DiChiara,T.J. and Reinhart,P.H.
  TITLE     Cloning, expression, and distribution of functionally distinct
            Ca(2+)-activated K+ channel isoforms from human brain
  JOURNAL   Neuron 13 (6), 1315-1330 (1994)
   PUBMED   7993625
REFERENCE   8  (bases 1 to 11976)
  AUTHORS   Dworetzky,S.I., Trojnacki,J.T. and Gribkoff,V.K.
  TITLE     Cloning and expression of a human large-conductance
            calcium-activated potassium channel
  JOURNAL   Brain Res Mol Brain Res 27 (1), 189-193 (1994)
   PUBMED   7877450
REFERENCE   9  (bases 1 to 11976)
  AUTHORS   Pallanck,L. and Ganetzky,B.
  TITLE     Cloning and characterization of human and mouse homologs of the
            Drosophila calcium-activated potassium channel gene, slowpoke
  JOURNAL   Hum Mol Genet 3 (8), 1239-1243 (1994)
   PUBMED   7987297
REFERENCE   10 (bases 1 to 11976)
  AUTHORS   Knaus,H.G., Folander,K., Garcia-Calvo,M., Garcia,M.L.,
            Kaczorowski,G.J., Smith,M. and Swanson,R.
  TITLE     Primary sequence and immunological characterization of beta-subunit
            of high conductance Ca(2+)-activated K+ channel from smooth muscle
  JOURNAL   J Biol Chem 269 (25), 17274-17278 (1994)
   PUBMED   8006036
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL731556.11, AL627447.12,
            AC021032.9, AL157833.14, AL731560.4 and AC011439.12.
            
            On May 31, 2019 this sequence version replaced NM_001322829.1.
            
            Summary: This gene encodes the alpha subunit of calcium-activated
            BK channel. The encoded protein is involved in several
            physiological processes including smooth muscle contraction,
            neurotransmitter release and neuronal excitability. Mutations in
            this gene are associated with a spectrum of neurological disorders
            including Paroxysmal Nonkinesigenic Dyskinesia 3, Idiopathic
            Generalized Epilepsy 16 and Liang-Wang syndrome. [provided by
            RefSeq, Aug 2022].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-544               AL731556.11        78960-79503         c
            545-706             AL627447.12        52230-52391         c
            707-768             AC021032.9         8165-8226
            769-862             AC021032.9         74505-74598
            863-974             AC021032.9         75889-76000
            975-1050            AC021032.9         138373-138448
            1051-1126           AC021032.9         146979-147054
            1127-1297           AC021032.9         149078-149248
            1298-1389           AC021032.9         150849-150940
            1390-1500           AL157833.14        10959-11069         c
            1501-1606           AL157833.14        7047-7152           c
            1607-1689           AL157833.14        5196-5278           c
            1690-1759           AL731560.4         100456-100525       c
            1760-1915           AL731560.4         94072-94227         c
            1916-2025           AL731560.4         60503-60612         c
            2026-2094           AL731560.4         48798-48866         c
            2095-2181           AL731560.4         39988-40074         c
            2182-2258           AL731560.4         32942-33018         c
            2259-2267           AL731560.4         29160-29168         c
            2268-2361           AC011439.12        169558-169651       c
            2362-2485           AC011439.12        153385-153508       c
            2486-2710           AC011439.12        148726-148950       c
            2711-2903           AC011439.12        144357-144549       c
            2904-3017           AC011439.12        114520-114633       c
            3018-3148           AC011439.12        109550-109680       c
            3149-3343           AC011439.12        91109-91303         c
            3344-3462           AC011439.12        89035-89153         c
            3463-3687           AC011439.12        86875-87099         c
            3688-11976          AC011439.12        69186-77474         c
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.3"
     gene            1..11976
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="potassium calcium-activated channel subfamily M
                     alpha 1"
                     /db_xref="GeneID:3778"
                     /db_xref="HGNC:HGNC:6284"
                     /db_xref="MIM:600150"
     exon            1..544
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..3709
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="isoform g is encoded by transcript variant 10; BKCA
                     alpha subunit; potassium channel, calcium activated large
                     conductance subfamily M alpha, member 1; stretch-activated
                     Kca channel; maxi-K channel HSLO; calcium-activated
                     potassium channel, subfamily M subunit alpha-1; potassium
                     large conductance calcium-activated channel, subfamily M,
                     alpha member 1; big potassium channel alpha subunit;
                     k(VCA)alpha; slo homolog; slowpoke homolog; BK channel
                     alpha subunit"
                     /codon_start=1
                     /product="Calcium-activated potassium channel subunit
                     alpha-1 isoform g"
                     /protein_id="NP_001309758.1"
                     /db_xref="GeneID:3778"
                     /db_xref="HGNC:HGNC:6284"
                     /db_xref="MIM:600150"
                     /translation="MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSS
                     SSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGL
                     FIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEAVAAEVGWMT
                     SVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQID
                     MAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRA
                     LRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN
                     QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIG
                     NRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNL
                     ELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMR
                     VISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQ
                     GLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFV
                     KLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHD
                     DITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPG
                     NDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSA
                     LIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRAD
                     LRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGF
                     TPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELY
                     LTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGG
                     YSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA
                     HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSK
                     KSSSVHSIPSTANRQNRPKSRESRDKQKKEMVYR"
     exon            545..706
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..768
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..862
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            863..974
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1050
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1126
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1297
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1389
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1500
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1606
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1689
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1759
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1915
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2025
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2094
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2181
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2258
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2267
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2361
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..2485
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2710
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2903
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3017
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3148
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3343
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3462
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3687
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..11976
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11947..11952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="hexamer: ATTAAA"
     polyA_site      11976
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="major polyA site"
ORIGIN      
        1 gcacagcgcc ccgagcccag gcgcctcccc gcccccctcc cgcgctccgc ggcggcggcg
       61 gcggcggcag cagtagcagc aatatggctg ttgatgggtg tttggggtgg cgctggcggc
      121 gggaggagct cccccgagcc cctgcgccgg ctgcccgttg ctagctatgg caaatggtgg
      181 cggcggcggc ggcggcagca gcggcggcgg cggcggcggc ggaggcagca gtcttagaat
      241 gagtagcaat atccacgcga accatctcag cctagacgcg tcctcctcct cctcctcctc
      301 ctcttcctct tcttcttctt cctcctcctc ttcctcctcg tcctcggtcc acgagcccaa
      361 gatggatgcg ctcatcatcc cggtgaccat ggaggtgccg tgcgacagcc ggggccaacg
      421 catgtggtgg gctttcctgg cctcctccat ggtgactttc ttcgggggcc tcttcatcat
      481 cttgctctgg cggacgctca agtacctgtg gaccgtgtgc tgccactgcg ggggcaagac
      541 gaaggaggcc cagaagatta acaatggctc aagccaggcg gatggcactc tcaaaccagt
      601 ggatgaaaaa gaggaggcag tggccgccga ggtcggctgg atgacctccg tgaaggactg
      661 ggcgggggtg atgatatccg cccagacact gactggcaga gtcctggttg tcttagtctt
      721 tgctctcagc atcggtgcac ttgtaatata cttcatagat tcatcaaacc caatagaatc
      781 ctgccagaat ttctacaaag atttcacatt acagatcgac atggctttca acgtgttctt
      841 ccttctctac ttcggcttgc ggtttattgc agccaacgat aaattgtggt tctggctgga
      901 agtgaactct gtagtggatt tcttcacggt gccccccgtg tttgtgtctg tgtacttaaa
      961 cagaagttgg cttggtttga gatttttaag agctctgaga ctgatacagt tttcagaaat
     1021 tttgcagttt ctgaatattc ttaaaacaag taattccatc aagctggtga atctgctctc
     1081 catatttatc agcacgtggc tgactgcagc cgggttcatc catttggtgg agaattcagg
     1141 ggacccatgg gaaaatttcc aaaacaacca ggctctcacc tactgggaat gtgtctattt
     1201 actcatggtc acaatgtcca ccgttggtta tggggatgtt tatgcaaaaa ccacacttgg
     1261 gcgcctcttc atggtcttct tcatcctcgg gggactggcc atgtttgcca gctacgtccc
     1321 tgaaatcata gagttaatag gaaaccgcaa gaaatacggg ggctcctata gtgcggttag
     1381 tggaagaaag cacattgtgg tctgcggaca catcactctg gagagtgttt ccaacttcct
     1441 gaaggacttt ctgcacaagg accgggatga cgtcaatgtg gagatcgttt ttcttcacaa
     1501 catctccccc aacctggagc ttgaagctct gttcaaacga cattttactc aggtggaatt
     1561 ttatcagggt tccgtcctca atccacatga tcttgcaaga gtcaagatag agtcagcaga
     1621 tgcatgcctg atccttgcca acaagtactg cgctgacccg gatgcggagg atgcctcgaa
     1681 tatcatgaga gtaatctcca taaagaacta ccatccgaag ataagaatca tcactcaaat
     1741 gctgcagtat cacaacaagg cccatctgct aaacatcccg agctggaatt ggaaagaagg
     1801 tgatgacgca atctgcctcg cagagttgaa gttgggcttc atagcccaga gctgcctggc
     1861 tcaaggcctc tccaccatgc ttgccaacct cttctccatg aggtcattca taaagattga
     1921 ggaagacaca tggcagaaat actacttgga aggagtctca aatgaaatgt acacagaata
     1981 tctctccagt gccttcgtgg gtctgtcctt ccctactgtt tgtgagctgt gttttgtgaa
     2041 gctcaagctc ctaatgatag ccattgagta caagtctgcc aaccgagaga gccgtatatt
     2101 aattaatcct ggaaaccatc ttaagatcca agaaggtact ttaggatttt tcatcgcaag
     2161 tgatgccaaa gaagttaaaa gggcattttt ttactgcaag gcctgtcatg atgacatcac
     2221 agatcccaaa agaataaaaa aatgtggctg caaacggctg atatattttg aagatgagca
     2281 gccgtcaaca ctatcaccaa aaaaaaagca acggaatgga ggcatgcgga actcacccaa
     2341 cacctcgcct aagctgatga ggcatgaccc cttgttaatt cctggcaatg atcagattga
     2401 caacatggac tccaatgtga agaagtacga ctctactggg atgtttcact ggtgtgcacc
     2461 caaggagata gagaaagtca tcctgactcg aagtgaagct gccatgaccg tcctgagtgg
     2521 ccatgtcgtg gtctgcatct ttggcgacgt cagctcagcc ctgatcggcc tccggaacct
     2581 ggtgatgccg ctccgtgcca gcaactttca ttaccatgag ctcaagcaca ttgtgtttgt
     2641 gggctctatt gagtacctca agcgggaatg ggagacgctt cataacttcc ccaaagtgtc
     2701 catattgcct ggtacgccat taagtcgggc tgatttaagg gctgtcaaca tcaacctctg
     2761 tgacatgtgc gttatcctgt cagccaatca gaataatatt gatgatactt cgctgcagga
     2821 caaggaatgc atcttggcgt cactcaacat caaatctatg cagtttgatg acagcatcgg
     2881 agtcttgcag gctaattccc aagggttcac acctccagga atggatagat cctctccaga
     2941 taacagccca gtgcacggga tgttacgtca accatccatc acaactgggg tcaacatccc
     3001 catcatcact gaactagtga acgatactaa tgttcagttt ttggaccaag acgatgatga
     3061 tgaccctgat acagaactgt acctcacgca gccctttgcc tgtgggacag catttgccgt
     3121 cagtgtcctg gactcactca tgagcgcgac gtacttcaat gacaatatcc tcaccctgat
     3181 acggaccctg gtgaccggag gagccacgcc ggagctggag gctctgattg ctgaggaaaa
     3241 cgcccttaga ggtggctaca gcaccccgca gacactggcc aatagggacc gctgccgcgt
     3301 ggcccagtta gctctgctcg atgggccatt tgcggactta ggggatggtg gttgttatgg
     3361 tgatctgttc tgcaaagctc tgaaaacata taatatgctt tgttttggaa tttaccggct
     3421 gagagatgct cacctcagca cccccagtca gtgcacaaag aggtatgtca tcaccaaccc
     3481 gccctatgag tttgagctcg tgccgacgga cctgatcttc tgcttaatgc agtttgacca
     3541 caatgccggc cagtcccggg ccagcctgtc ccattcctcc cactcgtcgc agtcctccag
     3601 caagaagagc tcctctgttc actccatccc atccacagca aaccgacaga accggcccaa
     3661 gtccagggag tcccgggaca aacagaaaaa agaaatggtt tacagatgaa ccggataatg
     3721 cctatcccag aaacattcaa atcaagccca tgagtaccca catggctaac cagatcaacc
     3781 aatataaatc cacaagcagc ttgattccac caatcagaga agttgaagat gaatgttgac
     3841 tcccaggaga ccagaactat ttttttaaag cctgacaaac ttcataaatg gtgatgtgac
     3901 ttttcttctt acatgctgaa tcactggtgg aaacgttagg ataagcaaga attgccagaa
     3961 aataaagtag acagatcttt ctctttgtct gccttgaata ttgcttccat gtattttgga
     4021 aaagaaaatg atttcattta taaatgaaca tactaaaata gtcatgtata gcctctagca
     4081 ttttaaatta tttttattta ttagaaagaa aatatatttt ttcttttcat tgtcaaatat
     4141 cttccctata tctaaacaat gcaaaatcta aatgaataag tggtcagact ccttaatgtg
     4201 tttttctctt ctttctctct ttttctttga ggagaatgat ggtcaccacc acaattaatt
     4261 gtaattaagg gaaatgaatt attaaaacaa ttttaaaatg gccaatgatg tatacatgta
     4321 ttttagtaaa tccagaaaag aagactaagg ggacaggtga atacctgctc ctcgcctgga
     4381 tgggtgtgta ttttgatctg cattgacacc agctcttaat aaatggaaac ttatttattt
     4441 tcacagttga aagtcatatt tttgtagttc tagttttcat tctgtagttc tcaccccttt
     4501 gttcatattt ttaaagggaa gaccttctgc tgcttttcta caattggcag atcaggtttg
     4561 tctggccaac taaggacacc aggagggttg tgggcacacc tgggatgtgg aagctgagga
     4621 ccagatgcac acaaggcatt gctctgtttg ctgttctagt ggtggagact cctacgtgct
     4681 gttatgcttc tcattttata tttccaaacc acctgagcga gtaatgtcaa cttcgggagg
     4741 gtctggtgga ctttctcccc actccccaag tctcagagct tttagcctgg aagtttgtaa
     4801 aactgattag tagttttact ttaccatagg atgtgagagg gcagtcattc ttctcactga
     4861 gtttttgttt aaaaaccagg aaatttctac aatcatggag acagatttta atttgcatgc
     4921 tctcctttat ttagttgctg gggccaaaca catgaacttg ttacaacaat ttaaaaataa
     4981 taataacaaa aggaaagcaa cgaatgcact tcatatagac tgttagaaaa ggcctttgaa
     5041 aattaccagc agtgaaatca aataaattag ccactttaaa ctgcggtcaa aagatttttc
     5101 tagccatcag atgcaattac gatgtctcag agctctgcta tcgcttgcat gctcggtgta
     5161 cagtctcccg gcaactgttt tgttccaatc aacttagcaa gtctttcaac tttaaaatta
     5221 tcaatctttg atatgatcat caaggtctct ggagaagcat ggatgtaata gtgatttcta
     5281 ttttcttaaa aaatgtaaac tgtggacact taatggaacc aagctaacca aagtatctcc
     5341 ccgaagtttc atgatatcta gtccattaac tctttgggcc atgacctgtg gtacctagtt
     5401 atggtagtgc aaatcagatc agagcaaagc aatgagatca tcatgaagac atatgtccac
     5461 taagtactcc aattaccagc gtaggcaaaa tgagcttcca cctctgtcca ctccctgcag
     5521 agccaatata gtcgtgcttc cacctcgtag gcattgtcct gcagtgattt gtcggactgt
     5581 ggttactctg tcgtcacatt ttgtagaatt agttctataa aagtattgtg aatacaatgt
     5641 atgtccaatt ggattgttta aaaactactt ataattaatc actagcctac tcagcagact
     5701 aaaatgatgt caacagtcta tattcaggct tataaacatt tcttggtttc caacagaagt
     5761 caaatgtgtt tgtggggaga acgttttgct aggattttga caactttcct gaaagctagg
     5821 ccttttttac ataatgattt tgcattgggg tcaccttagt atattcacgt caatccacgc
     5881 tcttctttcc ttcgtgaacc atagtgggct cttcactcct gctcgtaata accacctcca
     5941 aatttgttag agtgatttgg tctcctctgg caggctgtgg tggccacata aaggatcttc
     6001 cattagaggg acacaggaca caaagctcca ttcccatgac cctctcttcc ctgtgtctcc
     6061 cctaacaagg caatctggaa gcagaaatgc aaactccagc cccaccacac atccaccgat
     6121 gcatttgtgg ctagaaattt taatctaaac tttttttaaa aaaaatcata atagttagga
     6181 tagtttccta tagcaaaggg ctccttgatt cataagcacc tcccccccaa aaaaaccaaa
     6241 ttggtaaaaa ttcattttct tttaaataga gcaataagtt catactgtgt tgccgttgta
     6301 ataatgaatt gttctggact agaaaacaaa atgaggcatt ttgttaaggg ggtaggaaaa
     6361 tgactgattg ttgggccctt tggcaaaagc tggtgcctgg taagtagaaa cgaaatggaa
     6421 acagaaataa catttgtgaa atcactggag ggctcaagca aatgccagag cttctccctg
     6481 gcttgttcct acttgttgag ctctttggcc ctgagtccca tccatctcca ctgcaacagt
     6541 ctgtctttct gcctacatcc gcctgagacc aaggagggtc tttggtacag tccacattat
     6601 gaggctctga tttcctatcc cagtttaggg ctccagaaaa gccattctcc caaactaatg
     6661 attcttgggt gctgtgggtc accaacaggc tgtttacaaa gcagaaaata tcatcaccat
     6721 cataaccttt ctccttgaga aaacttttat ttttgtctaa gcaattttat attttagaat
     6781 atcatcttga accattataa aacaccttaa ataaaagtga ttgtgactaa ggcacgttgg
     6841 agaacattcc aatctttcct cccaagggtt ctggcacctt atgatgtact ttgtaaaaaa
     6901 attgattgaa gattttaaat aaacaggaaa ttagaagtgt tagggttaga taaaggaaaa
     6961 gtgaaaaagc ataagaaatt tgggactttt tcatgttgaa aggagaaaaa gcacaagttc
     7021 cctagttacc atttaatatg agggtgcaag agccacattt acaaacaaca aacttgatct
     7081 ttaaaaatgt ttttaaaact ctctgagtct cagtgacttc atttgtaaat gagagtgatt
     7141 catctagatg actctaaggt ctctttcagg tcccagggga cgtgatctaa gttaggagtg
     7201 ggaacatggg agtggctgct tctccttggc taagcccatg cagactattg tgtccaggac
     7261 tggaataaag gtcctttttg ctgcatcacc caggcaacac tcccagcctt actcaggagt
     7321 ggtgaacatt ttctgctctg gacatagaat aggtaggttc attcctggtc ttttcacttt
     7381 agattctctt taggttcaag atgcagattt ctcaatttgg acacacctgc atcacgttgg
     7441 tcaaattttg ctgcctgttt ctagggtttt cttctcagtt tcatccctgc cttccctttt
     7501 ttgttgtttt ttttttggtt caaggcccag cttcagttaa tcccttcatc tctatgccat
     7561 gtctccaatt cctagtatgt aactagaggt tcaagagacc ccaaattcct gccattttcc
     7621 ccatttatgt ttccccatgt gaatcttcag gaagtaacca gtcttccttc tcacttcatt
     7681 tcctgtcctc tgtttatttg gttattttct ttcattaata aactaaccat ctcttagcta
     7741 cttacattca caaatgtaat tttaatctat tgtgtttcat tgtgaaagag aatctcattg
     7801 aaggaaggaa ggaagaaatt gaggaataaa ggtaggaagg gagagaaaca aagactatcc
     7861 aaccaattga ctgagatgtg agccggcttg actcctagga gactaagtag atccattcag
     7921 cttgaacact gagttccagg gcagaatctg tccattttct gttttgttct tacttgctgc
     7981 ttttggaatg gtggtaaagc caccagtcac catgagcctt gaaaggacct tcactaatga
     8041 ggacttgact caccctcttt aaaaagcaaa agaaacagaa tttacttaca atcttctggt
     8101 aaattacctt tctgatcctc tccttattcc caaaaggcaa tttttttaat ccagcagaga
     8161 tgatttagca tataaatccc aggtcatttg aggaggtgat gacgctattt ggatttattt
     8221 atgagacagt agtaattata aatgattcct aggcagtttg tactagtttg gtcatattgg
     8281 ttgtttaagt ggagtctctt ctgattgttt gtttccaata aagtatcagt tgttaataat
     8341 ttcaagattt ccataaaaat aagaaaactt ggaaaacatt tttaatttta tttcttaaga
     8401 gaccacagag cctaattttc aatttttatg gtaagcttgc ttaaaacatg tataaaaaga
     8461 agcctacatg ctcaaaggca gaggcacctt tagccctaca acagttggaa gctccatgat
     8521 atcacagccc cacatggata gattggagaa agaattctga cttagaccac ctgtagcaga
     8581 ccttcctctt ttggcatatt tttttaatca caaaaaggac ctgagactcc ctatctaaag
     8641 ataagccaaa cttggggtat acattaagaa tctgggaaga aagaaaaaaa agaaaaagat
     8701 ataataagca gtattttaaa ataccatgag ttatgggcat gcatatggta caaagagtag
     8761 ttctttgggt tgaaagaaag aagagtttgc attgaactgc gtaggctggc aagaatgcag
     8821 aggtatacaa tgtttgatgg gctttggagg catttcaata gtaaatgggt gacaggactg
     8881 gaaagatgtg gaatatgagg gttttccagg gagatgggac ttaatgggcc atgatgtatt
     8941 tgtaggaatg tgatttcaat agattgacta ttttgtctac tgtgataagt ttgtgtaggg
     9001 gaactgagga aaactgagat gagacttcat ggaaggggct acaaagtaca gtctaaaggc
     9061 ctagataaag tttgaaactt gatttagaaa gtcatgaaaa gccactcaga gttttaagca
     9121 aagagtgatg gggcagcaat ggctgtgcat ttttcaggtt gaaagtagtg agatgtcagt
     9181 tcttcctgga agatatcatg agaatgcaca caaaactgaa cattttctca gcagcaagga
     9241 gtgctaaagg gtgcacactc caagtcaaca actcaccgtg tgcagcacct tcctcacaca
     9301 ttttgctttg ctttctgtca caggaccatc agacgtagaa cgtcctgact ctcctgaatt
     9361 actagaatgt cagcaacaaa ctcacaaagt agattagagg gtaggaatag atacataaat
     9421 ttttaaaaac ctgccaatca tactgttttc tagacagtaa tataataaga acatgaggca
     9481 ggacatccac aaatagtaga gaataattaa tagtccagaa ctgccaaata ggaattcccc
     9541 ttatgaaaac acccttgcag ctaatcaggt agcccttgtt tagaaaccaa ttagcactca
     9601 taaaatattt gtacaaggag tgagtggggg agaaatgagc acaatatggg ttctgccatc
     9661 aatcagctta taatacaact aaagaaagaa aacatgctca aagcaccatt agagaaaggg
     9721 tgagccctaa tgacatagtt tcacattcaa tatggtagga gttgaaacaa gggagaaatg
     9781 ctgtggtttg aagtggttca acaaggtagg atttctagag aaaggggggt ttaggagtta
     9841 gataagtaga ggatcagcat tctaggtatg aaggcaggat tatctggcat gtggcagggt
     9901 gaatgatgtt aatcagtaac tgctaattga tatttactga gcagctgctg tgtcctcacg
     9961 atggtaagac tattagagga agacagccca cctgcctaac tgggaacaca aggcacacat
    10021 atattggaaa gatcatgttg cacaaaacag gtgttcaata aataattttt gaataaatgt
    10081 gaacactgaa tagcaaataa atgttataga tcgtaatagt tgtagaagat tggttgggat
    10141 tgtagggact ggatgtgggg caatggctgt gggtctagag tatattcatt gtgcagtgga
    10201 cacacaccta atctgtcctc aaggacagaa ccatgtggag gaataggaaa acaagagaag
    10261 caggcaagaa gcggaggcag tgagtacagc agctaagaaa tgttacattg ggttcatcca
    10321 gagcaagaaa agctgagagc tgtccgtgag ggaaggccac acaggcaaga ggagtgggac
    10381 aggggagtaa aggagtgggc tacaacaggc cctgcagatg tggaagccag acccacagag
    10441 gcacttagaa agtgaggatc ctggatgaat tattttaggg agaaagtgta ggtttagatc
    10501 agctggcaga tgtgtttgtg ggctacagcc aagtttttca ggactaagaa ggagcaaaat
    10561 aagtagaact tcttgggaca ggagatctag ggtactccag aaccagggaa atgagaaaca
    10621 agaggggaat tttaagaaag agagggaaga tcatctatga ggttggagat gaaaggtgtt
    10681 ggagatggtg aggcacaagt caaaagactc actcgaaggg caggaactct gcttatttgt
    10741 ccatattccc agagtttgtc atgaggtctg gcccacgtgg gcagtcactc ggccactatg
    10801 tatcactgtg gctgccatgg tggatggagg ctggacagaa cctgtggtga tcggcagtct
    10861 ttctggagga ctcaccaggg aggggtctca gggtgggcat ctcctttcac cttcctgtgc
    10921 atgtcactgc actgttcttc ttccctccct gaacagcaca cacactgcat ccttagtttc
    10981 aggggtctga aaacaaatgc tacgaccacc ttgctttggt ggcttttctg aacaggcatt
    11041 gtgagaagag atgttctggc caggtgaaag tggactttat ttttaggtag gtttttcagt
    11101 ttagaaaggt ttcctttgct tatcccttga ggacttctgt gctgctgctt ctcctccatc
    11161 ttgctagtta acagaacagg ggcagaagtg acaccaatgc tctcggccat gcctgccttg
    11221 ggaagcacta ccgggcccca ctgtttagaa tcttagatgg aaatgtacac aaggcattga
    11281 cacacgcctg cgggagtgac ccagtgcttc cctcccatgg gactcagggt cagcgtggga
    11341 aggcagctgg cagggacagg agccccgccc gcaaggtgga ctgagcctca cttccacaca
    11401 aaaccagagt cccatctgcc ccagagcgct cgctctcctc cccttccttc cgctcaatgc
    11461 agcctgacac gcctttgacc agaactgcaa catttcaaac acaaacccag atctgttggg
    11521 aaatcctaat taaaaacgtt gcattcccag gtggcacaat cacatcctgc ctaaaacttc
    11581 tggcccacat cccaccaccc agatgttgaa aagaaacctt tctctgctta acaccaaaac
    11641 ctgcacttca aggtttcttt aggggacaaa gaaaaaaaaa gtacacacat gcagagttca
    11701 gcctacatgt tcattgagga aaaagagcgt ggcttattca tctttcaatc ttttgattgt
    11761 tggcattatg attatgatta tgattaatta ctctgacctg acaggaattg aagaagagac
    11821 tatatgctgg tgctctgaaa tgatctaccc aactctgtca tctgtaacaa ccagtgataa
    11881 ctctcttgtc ttgtaaaaag ggtttgtaca taacttgtac atggtttcat tttgtatttt
    11941 tcgcagatta aaaatttatg tatttgtgtt ctaaaa
//
