LOCUS       NM_001161352            6261 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens potassium calcium-activated channel subfamily M alpha
            1 (KCNMA1), transcript variant 3, mRNA.
ACCESSION   NM_001161352
VERSION     NM_001161352.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Dinsdale,R.L. and Meredith,A.L.
  TITLE     Evaluation of four KCNMA1 channelopathy variants on BK channel
            current under CaV1.2 activation
  JOURNAL   Channels (Austin) 18 (1), 2396346 (2024)
   PUBMED   39217512
  REMARK    GeneRIF: Evaluation of four KCNMA1 channelopathy variants on BK
            channel current under CaV1.2 activation.
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Li,Q., Chen,G. and Yan,J.
  TITLE     Transmembrane determinants of voltage-gating differences between BK
            (Slo1) and Slo3 channels
  JOURNAL   Biophys J 123 (14), 2154-2166 (2024)
   PUBMED   38637987
  REMARK    GeneRIF: Transmembrane determinants of voltage-gating differences
            between BK (Slo1) and Slo3 channels.
REFERENCE   3  (bases 1 to 6261)
  AUTHORS   Moldenhauer,H.J., Tammen,K. and Meredith,A.L.
  TITLE     Structural mapping of patient-associated KCNMA1 gene variants
  JOURNAL   Biophys J 123 (14), 1984-2000 (2024)
   PUBMED   38042986
  REMARK    GeneRIF: Structural mapping of patient-associated KCNMA1 gene
            variants.
REFERENCE   4  (bases 1 to 6261)
  AUTHORS   Dinsdale,R.L., Roache,C.E. and Meredith,A.L.
  TITLE     Disease-associated KCNMA1 variants decrease circadian clock
            robustness in channelopathy mouse models
  JOURNAL   J Gen Physiol 155 (11) (2023)
   PUBMED   37728576
  REMARK    GeneRIF: Disease-associated KCNMA1 variants decrease circadian
            clock robustness in channelopathy mouse models.
REFERENCE   5  (bases 1 to 6261)
  AUTHORS   Ohya,S., Kajikuri,J., Kito,H. and Matsui,M.
  TITLE     Down-Regulation of CYP3A4 by the KCa1.1 Inhibition Is Responsible
            for Overcoming Resistance to Doxorubicin in Cancer Spheroid Models
  JOURNAL   Int J Mol Sci 24 (21), 15672 (2023)
   PUBMED   37958656
  REMARK    GeneRIF: Down-Regulation of CYP3A4 by the KCa1.1 Inhibition Is
            Responsible for Overcoming Resistance to Doxorubicin in Cancer
            Spheroid Models.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6261)
  AUTHORS   McCobb,D.P., Fowler,N.L., Featherstone,T., Lingle,C.J., Saito,M.,
            Krause,J.E. and Salkoff,L.
  TITLE     A human calcium-activated potassium channel gene expressed in
            vascular smooth muscle
  JOURNAL   Am J Physiol 269 (3 Pt 2), H767-H777 (1995)
   PUBMED   7573516
REFERENCE   7  (bases 1 to 6261)
  AUTHORS   Tseng-Crank,J., Foster,C.D., Krause,J.D., Mertz,R., Godinot,N.,
            DiChiara,T.J. and Reinhart,P.H.
  TITLE     Cloning, expression, and distribution of functionally distinct
            Ca(2+)-activated K+ channel isoforms from human brain
  JOURNAL   Neuron 13 (6), 1315-1330 (1994)
   PUBMED   7993625
REFERENCE   8  (bases 1 to 6261)
  AUTHORS   Dworetzky,S.I., Trojnacki,J.T. and Gribkoff,V.K.
  TITLE     Cloning and expression of a human large-conductance
            calcium-activated potassium channel
  JOURNAL   Brain Res Mol Brain Res 27 (1), 189-193 (1994)
   PUBMED   7877450
REFERENCE   9  (bases 1 to 6261)
  AUTHORS   Pallanck,L. and Ganetzky,B.
  TITLE     Cloning and characterization of human and mouse homologs of the
            Drosophila calcium-activated potassium channel gene, slowpoke
  JOURNAL   Hum Mol Genet 3 (8), 1239-1243 (1994)
   PUBMED   7987297
REFERENCE   10 (bases 1 to 6261)
  AUTHORS   Knaus,H.G., Folander,K., Garcia-Calvo,M., Garcia,M.L.,
            Kaczorowski,G.J., Smith,M. and Swanson,R.
  TITLE     Primary sequence and immunological characterization of beta-subunit
            of high conductance Ca(2+)-activated K+ channel from smooth muscle
  JOURNAL   J Biol Chem 269 (25), 17274-17278 (1994)
   PUBMED   8006036
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC024965.1, AB113382.1,
            U11058.2, AC011439.12 and BQ574058.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001161352.1.
            
            Summary: This gene encodes the alpha subunit of calcium-activated
            BK channel. The encoded protein is involved in several
            physiological processes including smooth muscle contraction,
            neurotransmitter release and neuronal excitability. Mutations in
            this gene are associated with a spectrum of neurological disorders
            including Paroxysmal Nonkinesigenic Dyskinesia 3, Idiopathic
            Generalized Epilepsy 16 and Liang-Wang syndrome. [provided by
            RefSeq, Aug 2022].
            
            Transcript Variant: This variant (3) differs in the 3' UTR and has
            multiple coding region differences, compared to variant 1. The
            resulting isoform (c) contains a distinct C-terminus, compared to
            isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB113382.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286628.14/ ENSP00000286628.8
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               BC024965.1         12-235
            225-3975            AB113382.1         1-3751
            3976-5833           U11058.2           3527-5384
            5834-5834           AC011439.12        84893-84893         c
            5835-6261           BQ574058.1         23-449              c
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.3"
     gene            1..6261
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="potassium calcium-activated channel subfamily M
                     alpha 1"
                     /db_xref="GeneID:3778"
                     /db_xref="HGNC:HGNC:6284"
                     /db_xref="MIM:600150"
     exon            1..544
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..3877
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="isoform c is encoded by transcript variant 3; BKCA
                     alpha subunit; potassium channel, calcium activated large
                     conductance subfamily M alpha, member 1; stretch-activated
                     Kca channel; maxi-K channel HSLO; calcium-activated
                     potassium channel, subfamily M subunit alpha-1; potassium
                     large conductance calcium-activated channel, subfamily M,
                     alpha member 1; big potassium channel alpha subunit;
                     k(VCA)alpha; slo homolog; slowpoke homolog; BK channel
                     alpha subunit"
                     /codon_start=1
                     /product="Calcium-activated potassium channel subunit
                     alpha-1 isoform c"
                     /protein_id="NP_001154824.1"
                     /db_xref="CCDS:CCDS60569.1"
                     /db_xref="GeneID:3778"
                     /db_xref="HGNC:HGNC:6284"
                     /db_xref="MIM:600150"
                     /translation="MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSS
                     SSSSSSSSSSSSSSSSVHEPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGL
                     FIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADGTLKPVDEKEEAVAAEVGWMT
                     SVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQID
                     MAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRA
                     LRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN
                     QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIG
                     NRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNL
                     ELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMR
                     VISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQ
                     GLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFV
                     KLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHD
                     DITDPKRIKKCGCKRPKMSIYKRMRRACCFDCGRSERDCSCMSGRVRGNVDTLERAFP
                     LSSVSVNDCSTSFRAFEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQ
                     IDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIG
                     LRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRA
                     VNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPP
                     GMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQ
                     PFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYST
                     PQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLS
                     TPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSS
                     SVHSIPSTANRQNRPKSRESRDKQKYVQEERL"
     exon            545..706
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..768
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..862
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            863..974
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1050
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1126
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1297
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1389
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1500
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1606
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1689
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1759
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1915
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2025
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2094
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2181
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2258
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2432
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2526
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2650
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2875
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            2876..3068
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..3182
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3313
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3314..3508
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3509..3627
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     exon            3628..6261
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6239..6244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="hexamer: ATTAAA"
     polyA_site      6261
                     /gene="KCNMA1"
                     /gene_synonym="bA205K10.1; BKTM; CADEDS; hSlo; IEG16;
                     KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA;
                     SLO1"
                     /note="major polyA site"
ORIGIN      
        1 gcacagcgcc ccgagcccag gcgcctcccc gcccccctcc cgcgctccgc ggcggcggcg
       61 gcggcggcag cagtagcagc aatatggctg ttgatgggtg tttggggtgg cgctggcggc
      121 gggaggagct cccccgagcc cctgcgccgg ctgcccgttg ctagctatgg caaatggtgg
      181 cggcggcggc ggcggcagca gcggcggcgg cggcggcggc ggaggcagca gtcttagaat
      241 gagtagcaat atccacgcga accatctcag cctagacgcg tcctcctcct cctcctcctc
      301 ctcttcctct tcttcttctt cctcctcctc ttcctcctcg tcctcggtcc acgagcccaa
      361 gatggatgcg ctcatcatcc cggtgaccat ggaggtgccg tgcgacagcc ggggccaacg
      421 catgtggtgg gctttcctgg cctcctccat ggtgactttc ttcgggggcc tcttcatcat
      481 cttgctctgg cggacgctca agtacctgtg gaccgtgtgc tgccactgcg ggggcaagac
      541 gaaggaggcc cagaagatta acaatggctc aagccaggcg gatggcactc tcaaaccagt
      601 ggatgaaaaa gaggaggcag tggccgccga ggtcggctgg atgacctccg tgaaggactg
      661 ggcgggggtg atgatatccg cccagacact gactggcaga gtcctggttg tcttagtctt
      721 tgctctcagc atcggtgcac ttgtaatata cttcatagat tcatcaaacc caatagaatc
      781 ctgccagaat ttctacaaag atttcacatt acagatcgac atggctttca acgtgttctt
      841 ccttctctac ttcggcttgc ggtttattgc agccaacgat aaattgtggt tctggctgga
      901 agtgaactct gtagtggatt tcttcacggt gccccccgtg tttgtgtctg tgtacttaaa
      961 cagaagttgg cttggtttga gatttttaag agctctgaga ctgatacagt tttcagaaat
     1021 tttgcagttt ctgaatattc ttaaaacaag taattccatc aagctggtga atctgctctc
     1081 catatttatc agcacgtggc tgactgcagc cgggttcatc catttggtgg agaattcagg
     1141 ggacccatgg gaaaatttcc aaaacaacca ggctctcacc tactgggaat gtgtctattt
     1201 actcatggtc acaatgtcca ccgttggtta tggggatgtt tatgcaaaaa ccacacttgg
     1261 gcgcctcttc atggtcttct tcatcctcgg gggactggcc atgtttgcca gctacgtccc
     1321 tgaaatcata gagttaatag gaaaccgcaa gaaatacggg ggctcctata gtgcggttag
     1381 tggaagaaag cacattgtgg tctgcggaca catcactctg gagagtgttt ccaacttcct
     1441 gaaggacttt ctgcacaagg accgggatga cgtcaatgtg gagatcgttt ttcttcacaa
     1501 catctccccc aacctggagc ttgaagctct gttcaaacga cattttactc aggtggaatt
     1561 ttatcagggt tccgtcctca atccacatga tcttgcaaga gtcaagatag agtcagcaga
     1621 tgcatgcctg atccttgcca acaagtactg cgctgacccg gatgcggagg atgcctcgaa
     1681 tatcatgaga gtaatctcca taaagaacta ccatccgaag ataagaatca tcactcaaat
     1741 gctgcagtat cacaacaagg cccatctgct aaacatcccg agctggaatt ggaaagaagg
     1801 tgatgacgca atctgcctcg cagagttgaa gttgggcttc atagcccaga gctgcctggc
     1861 tcaaggcctc tccaccatgc ttgccaacct cttctccatg aggtcattca taaagattga
     1921 ggaagacaca tggcagaaat actacttgga aggagtctca aatgaaatgt acacagaata
     1981 tctctccagt gccttcgtgg gtctgtcctt ccctactgtt tgtgagctgt gttttgtgaa
     2041 gctcaagctc ctaatgatag ccattgagta caagtctgcc aaccgagaga gccgtatatt
     2101 aattaatcct ggaaaccatc ttaagatcca agaaggtact ttaggatttt tcatcgcaag
     2161 tgatgccaaa gaagttaaaa gggcattttt ttactgcaag gcctgtcatg atgacatcac
     2221 agatcccaaa agaataaaaa aatgtggctg caaacggccc aagatgtcca tctacaagag
     2281 aatgagacgg gcatgttgtt ttgattgcgg acgttctgag cgtgactgct catgcatgtc
     2341 aggccgtgtg cgtggtaacg tggacaccct tgagagagcc ttcccacttt cttctgtctc
     2401 tgttaatgat tgctccacca gtttccgtgc ctttgaagat gagcagccgt caacactatc
     2461 accaaaaaaa aagcaacgga atggaggcat gcggaactca cccaacacct cgcctaagct
     2521 gatgaggcat gaccccttgt taattcctgg caatgatcag attgacaaca tggactccaa
     2581 tgtgaagaag tacgactcta ctgggatgtt tcactggtgt gcacccaagg agatagagaa
     2641 agtcatcctg actcgaagtg aagctgccat gaccgtcctg agtggccatg tcgtggtctg
     2701 catctttggc gacgtcagct cagccctgat cggcctccgg aacctggtga tgccgctccg
     2761 tgccagcaac tttcattacc atgagctcaa gcacattgtg tttgtgggct ctattgagta
     2821 cctcaagcgg gaatgggaga cgcttcataa cttccccaaa gtgtccatat tgcctggtac
     2881 gccattaagt cgggctgatt taagggctgt caacatcaac ctctgtgaca tgtgcgttat
     2941 cctgtcagcc aatcagaata atattgatga tacttcgctg caggacaagg aatgcatctt
     3001 ggcgtcactc aacatcaaat ctatgcagtt tgatgacagc atcggagtct tgcaggctaa
     3061 ttcccaaggg ttcacacctc caggaatgga tagatcctct ccagataaca gcccagtgca
     3121 cgggatgtta cgtcaaccat ccatcacaac tggggtcaac atccccatca tcactgaact
     3181 agtgaacgat actaatgttc agtttttgga ccaagacgat gatgatgacc ctgatacaga
     3241 actgtacctc acgcagccct ttgcctgtgg gacagcattt gccgtcagtg tcctggactc
     3301 actcatgagc gcgacgtact tcaatgacaa tatcctcacc ctgatacgga ccctggtgac
     3361 cggaggagcc acgccggagc tggaggctct gattgctgag gaaaacgccc ttagaggtgg
     3421 ctacagcacc ccgcagacac tggccaatag ggaccgctgc cgcgtggccc agttagctct
     3481 gctcgatggg ccatttgcgg acttagggga tggtggttgt tatggtgatc tgttctgcaa
     3541 agctctgaaa acatataata tgctttgttt tggaatttac cggctgagag atgctcacct
     3601 cagcaccccc agtcagtgca caaagaggta tgtcatcacc aacccgccct atgagtttga
     3661 gctcgtgccg acggacctga tcttctgctt aatgcagttt gaccacaatg ccggccagtc
     3721 ccgggccagc ctgtcccatt cctcccactc gtcgcagtcc tccagcaaga agagctcctc
     3781 tgttcactcc atcccatcca cagcaaaccg acagaaccgg cccaagtcca gggagtcccg
     3841 ggacaaacag aagtacgtgc aggaagagcg gctttgatat gtgtatccac cgccactgtg
     3901 tgaaactgta tctgccactc atttccccag ttggtgtttc caacaaagta actttccctg
     3961 ttttcccctg tagtcccccc cttttttttt acacatattt gcatatgtat gatagtgtgc
     4021 atgtggttgt catttttatt tcaccaccat aaaacccttg agcacaacag caaataagca
     4081 gacggaccaa aagttattta tgattctggg ggaaaaataa cccaaaggca tgctccagac
     4141 ataaatagct cactgcagga acgagttcac agattagaag ggagcacttg tgatcaacgt
     4201 cagttaggca gagcaagttt atttaatgta aaagaaaagt tgattctgat ttatcaggat
     4261 tatcagggtg ctttgggttt tgattttgtt gttgttgttg ttttcctttc tttctttttt
     4321 tatacacaca ataagttagc acatgtttat ttgaaacaag caaccaaaca gcaatgaaaa
     4381 catattgatt gtttccagtc tctgggccga agtattgcga agcatttgaa aagctttcac
     4441 gatttgtgta gatgattatg aaggacctgc ttgttgcaag agaacatcag tgattttttt
     4501 agttactcac caaggccttt tgtcccagag ccagttccct ctgggagttc ttatgaacat
     4561 ttctcacctt aatatggagg agagaatagt attccaatca tggatgtatc aaattctagt
     4621 catttagttt aagtgaaaag aggtttgatt gcatattaaa ttgttattct gtctccttat
     4681 gttgccatat gaatagctat tttttttctt tcacttttga catttgggat gaaaagccat
     4741 atgtatcata aatatcagat gtaagtcatt aaaaactgcc ttcctgggac ttttacatct
     4801 tttaaaaggt gaattactta ccttatgtac agaataaata atgctcagga aagagcaagt
     4861 atttttccat gcattctcag gggatctttt tactcccctt tgtttgatta gttagggccc
     4921 caatgccagg taggaggaag ggctggggca atggtagagt gagaggaaga caaacccagc
     4981 tgcagatcat gcttttctag gagccgacat gctaaataaa ttagaatgta ggaggatcag
     5041 ccacagttga ctcaacaaag acaaaagcca gccaccacct tcaactgttg gcacagctgt
     5101 gcggtgctgg ctgtcccaat gcagaaagct ggtgggaagg aattcctcat catcactttc
     5161 tttaatgtag ccaatttagg cagggtaatg acggcaatag agagctgctc cttgtcatta
     5221 tgagacgtgg gataagaaga gtgcaacagt gagccaaaca cattttggta tagttatttt
     5281 tttcttcttt tgttttcttt cttttttaac acttagtaag catgagagga gaggtagaaa
     5341 aatacccttt tttcaacata tagttgtcag atgctttgtg catgcaaatc atgctttagg
     5401 cagtgcggta tttcttaaaa actggccaat tcaccataac caatttccct tatggatgga
     5461 ctaggctggt atatacatat ttgaaaagtt ttacttcaaa gaattccatc gaatagaata
     5521 ggggtaaaag ggaggaggaa aacatgtcac agctgtacca tctctaaaaa ggtgttttta
     5581 tggtgaatgt tttggattta gattttggat cccccgtccc ctcaagcatg atagttttgg
     5641 atatttgctt gctgtgtgaa ttgacaagca cttttactga caaatggtga ggctcagtca
     5701 gaacctccac cctcccccac accaaagaca ggggcagcgt agtattcaaa ccagtattgt
     5761 ggtggggaat aattgtatac atgtaaatta tcaagcccta tgagtggaag aattttttca
     5821 aattattttt gtccctctat atattgattt atattatgta taactatctc tttatataaa
     5881 ctatatataa ttatatatat ataactatat aattatatat atataactat atatataact
     5941 atatatatgt atcccctagt attggatcat gaagagctct tcatgcattc tttgcaaagg
     6001 aggttataaa gttacgccct cagaacattt ataactataa gaatgtgcca gttaaagtgc
     6061 tcaacaggaa atatgacagt ttaaaagcat tgtaaaactc acatagctta cttctctctc
     6121 taaagtgcaa caaggatgaa tagaatgggc caaggtatga caattaatgg ttctgcatga
     6181 cctagccact gctgggggtt ttcttctata acgttgtcct tgtgaaaact tttgtgaaat
     6241 taaaaaaaaa ggagttacaa a
//
