LOCUS       NM_004823               5721 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens potassium two pore domain channel subfamily K member 6
            (KCNK6), mRNA.
ACCESSION   NM_004823
VERSION     NM_004823.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Bobak,N., Feliciangeli,S., Chen,C.C., Ben Soussia,I., Bittner,S.,
            Pagnotta,S., Ruck,T., Biel,M., Wahl-Schott,C., Grimm,C., Meuth,S.G.
            and Lesage,F.
  TITLE     Recombinant tandem of pore-domains in a Weakly Inward rectifying K+
            channel 2 (TWIK2) forms active lysosomal channels
  JOURNAL   Sci Rep 7 (1), 649 (2017)
   PUBMED   28381826
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Zhao,K.Q., Xiong,G., Wilber,M., Cohen,N.A. and Kreindler,J.L.
  TITLE     A role for two-pore K channels in modulating Na absorption and Cl
            secretion in normal human bronchial epithelial cells
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 302 (1), L4-L12 (2012)
   PUBMED   21964404
  REMARK    GeneRIF: Potassium channels, in particular K2P channels, are
            expressed and functional in the apical membrane of airway
            epithelial cells
REFERENCE   3  (bases 1 to 5721)
  AUTHORS   Sebastiani,P., Solovieff,N., Hartley,S.W., Milton,J.N., Riva,A.,
            Dworkis,D.A., Melista,E., Klings,E.S., Garrett,M.E., Telen,M.J.,
            Ashley-Koch,A., Baldwin,C.T. and Steinberg,M.H.
  TITLE     Genetic modifiers of the severity of sickle cell anemia identified
            through a genome-wide association study
  JOURNAL   Am J Hematol 85 (1), 29-35 (2010)
   PUBMED   20029952
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   4  (bases 1 to 5721)
  AUTHORS   Gierten,J., Ficker,E., Bloehs,R., Schlomer,K., Kathofer,S.,
            Scholz,E., Zitron,E., Kiesecker,C., Bauer,A., Becker,R.,
            Katus,H.A., Karle,C.A. and Thomas,D.
  TITLE     Regulation of two-pore-domain (K2P) potassium leak channels by the
            tyrosine kinase inhibitor genistein
  JOURNAL   Br J Pharmacol 154 (8), 1680-1690 (2008)
   PUBMED   18516069
REFERENCE   5  (bases 1 to 5721)
  AUTHORS   Goldstein,S.A., Bayliss,D.A., Kim,D., Lesage,F., Plant,L.D. and
            Rajan,S.
  TITLE     International Union of Pharmacology. LV. Nomenclature and molecular
            relationships of two-P potassium channels
  JOURNAL   Pharmacol Rev 57 (4), 527-540 (2005)
   PUBMED   16382106
  REMARK    Review article
REFERENCE   6  (bases 1 to 5721)
  AUTHORS   Pountney,D.J., Gulkarov,I., Vega-Saenz de Miera,E., Holmes,D.,
            Saganich,M., Rudy,B., Artman,M. and Coetzee,W.A.
  TITLE     Identification and cloning of TWIK-originated similarity sequence
            (TOSS): a novel human 2-pore K+ channel principal subunit
  JOURNAL   FEBS Lett 450 (3), 191-196 (1999)
   PUBMED   10359073
REFERENCE   7  (bases 1 to 5721)
  AUTHORS   Salinas,M., Reyes,R., Lesage,F., Fosset,M., Heurteaux,C., Romey,G.
            and Lazdunski,M.
  TITLE     Cloning of a new mouse two-P domain channel subunit and a human
            homologue with a unique pore structure
  JOURNAL   J Biol Chem 274 (17), 11751-11760 (1999)
   PUBMED   10206991
REFERENCE   8  (bases 1 to 5721)
  AUTHORS   Chavez,R.A., Gray,A.T., Zhao,B.B., Kindler,C.H., Mazurek,M.J.,
            Mehta,Y., Forsayeth,J.R. and Yost,C.S.
  TITLE     TWIK-2, a new weak inward rectifying member of the tandem pore
            domain potassium channel family
  JOURNAL   J Biol Chem 274 (12), 7887-7892 (1999)
   PUBMED   10075682
  REMARK    Erratum:[J Biol Chem 1999 Aug 20;274(34):24440]
REFERENCE   9  (bases 1 to 5721)
  AUTHORS   Gray,A.T., Kindler,C.H., Sampson,E.R. and Yost,C.S.
  TITLE     Assignment of KCNK6 encoding the human weak inward rectifier
            potassium channel TWIK-2 to chromosome band 19q13.1 by radiation
            hybrid mapping
  JOURNAL   Cytogenet Cell Genet 84 (3-4), 190-191 (1999)
   PUBMED   10393428
REFERENCE   10 (bases 1 to 5721)
  AUTHORS   Mahboubi,S. and Kaufmann,J.H.
  TITLE     Intramural duodenal hematoma in children. The role of the
            radiologist in its conservative management
  JOURNAL   Gastrointest Radiol 1 (2), 167-171 (1976)
   PUBMED   1088718
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026806.5.
            
            On Nov 22, 2018 this sequence version replaced NM_004823.2.
            
            Summary: This gene encodes one of the members of the superfamily of
            potassium channel proteins containing two pore-forming P domains.
            This channel protein, considered an open rectifier, is widely
            expressed. It is stimulated by arachidonic acid, and inhibited by
            internal acidification and volatile anaesthetics. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF117708.1, AF134149.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263372.5/ ENSP00000263372.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               AC026806.5         13405-13832
            429-824             AC026806.5         20153-20548
            825-5721            AC026806.5         20740-25636
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..5721
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="potassium two pore domain channel subfamily K
                     member 6"
                     /db_xref="GeneID:9424"
                     /db_xref="HGNC:HGNC:6281"
                     /db_xref="MIM:603939"
     exon            1..428
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1048
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="inward rectifying potassium channel protein TWIK-2;
                     TWIK-originated sodium similarity sequence; K2P6.1
                     potassium channel; TWIK-originated similarity sequence;
                     potassium channel, two pore domain subfamily K, member 6"
                     /codon_start=1
                     /product="potassium channel subfamily K member 6"
                     /protein_id="NP_004814.1"
                     /db_xref="CCDS:CCDS12513.1"
                     /db_xref="GeneID:9424"
                     /db_xref="HGNC:HGNC:6281"
                     /db_xref="MIM:603939"
                     /translation="MRRGALLAGALAAYAAYLVLGALLVARLEGPHEARLRAELETLR
                     AQLLQRSPCVAAPALDAFVERVLAAGRLGRVVLANASGSANASDPAWDFASALFFAST
                     LITTVGYGYTTPLTDAGKAFSIAFALLGVPTTMLLLTASAQRLSLLLTHVPLSWLSMR
                     WGWDPRRAACWHLVALLGVVVTVCFLVPAVIFAHLEEAWSFLDAFYFCFISLSTIGLG
                     DYVPGEAPGQPYRALYKVLVTVYLFLGLVAMVLVLQTFRHVSDLHGLTELILLPPPCP
                     ASFNADEDDRVDILGPQPESHQQLSASSHTDYASIPR"
     exon            429..824
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..5721
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2180..2185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="hexamer: AATACA"
     polyA_site      2197
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="major polyA site"
     regulatory      2493..2498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="hexamer: AAGAAA"
     polyA_site      2510
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
     regulatory      5693..5698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="hexamer: AATAAA"
     polyA_site      5721
                     /gene="KCNK6"
                     /gene_synonym="K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2"
                     /note="major polyA site"
ORIGIN      
        1 gccgcgcctg tagcactccc ggaactggaa ctaggtgcca gacggtccgg aggcgggggc
       61 cacgtcagcg gggccaccca gggctcgcgg ggtcccggtg ggtgccatgc ggaggggcgc
      121 gcttctggcg ggcgccttgg ccgcgtacgc cgcgtacctg gtgctgggcg cgctgttggt
      181 ggcgcggctg gaggggccgc acgaagccag gctccgagcc gagctggaga cgctgcgggc
      241 gcagctgctt cagcgcagcc cgtgtgtggc tgcccccgcc ctggacgcct tcgtggagcg
      301 agtgctggcg gccggacggc tggggcgggt cgtgcttgct aacgcttcgg ggtccgccaa
      361 cgcctcggac cccgcctggg acttcgcctc tgctctcttc ttcgccagca cgctgatcac
      421 caccgtgggc tatgggtaca caacgccact gactgatgcg ggcaaggcct tctccatcgc
      481 ctttgcgctc ctgggcgtgc cgaccaccat gctgctgctg accgcctcag cccagcgcct
      541 gtcactgctg ctgactcacg tgcccctgtc ttggctgagc atgcgttggg gctgggaccc
      601 ccggcgggcg gcctgctggc acttggtggc cctgttgggg gtcgtagtga ccgtctgctt
      661 tctggtgccg gctgtgatct ttgcccacct cgaggaggcc tggagcttct tggatgcctt
      721 ctacttctgc tttatctctc tgtccaccat cggcctgggc gactacgtgc ccggggaggc
      781 ccctggccag ccctaccggg ccctctacaa ggtgctggtc acagtctacc tcttcctggg
      841 cctggtggcc atggtgctgg tgctgcagac cttccgccac gtgtccgacc tccacggcct
      901 cacggagctc atcctgctgc cccctccgtg ccctgccagt ttcaatgcgg atgaggacga
      961 tcgggtggac atcctgggcc cccagccgga gtcgcaccag caactctctg ccagctccca
     1021 caccgactac gcttccatcc ccaggtagct ggggcagcct ctgccaggct tgggtgtgcc
     1081 tggcctggga ctgaggggtc caggcgacca gagctggctg tacaggaatg tccacgagca
     1141 cagcaggtga tcttgaggcc ttgccgtcca ccgtctctcc tttgtttccc agcatctggc
     1201 tgggatgtga agggcagcac tccctgtccc catgtcccgg gctccactgg gcaccaacat
     1261 aaccttgttc tctgtccttt ctctcatcct ctttacactg tgtctctctg gctctctggc
     1321 attctcgctg cctctgtctt tccctcttgc tgtctctgtt tctcattctc tttcatgttc
     1381 cgtctgtgtc tctcaattaa ccactcgtca actgctgatt ctactgggct gtgggctcag
     1441 acctcatttc aggcaccaga ttggtcgcta caccctggac aagtgactgc ccgtctctga
     1501 gccttgattt cctcagctgc caaatgggaa gaatagaaga atttgcccct aaacccctcc
     1561 tgtgtgctgg ccctgtgcta gacagtgctg gagacatagt tgggggtgga gaactgccct
     1621 tatggagctt gcagtccagt gaggtggaca gacctgtccc cagacagtga tggcccaaaa
     1681 tggtcaggac tttaatggag gaggtgaggt gttgaaagca caggcagagt ggtcagggct
     1741 gaagtcggag aagcataggg actaggccca atccagcctg gaaagtcagg gaggacttcc
     1801 tagaggaagg gacatcgaac taagacctga actatgagaa ataggcagga agaagttgta
     1861 cctgactcat ttttctcagg tgtctccagg gagcaggacc catggaggga cccctggtgt
     1921 aggcctgggc gatagactct tcctcagcag cctggcaggc aggaaacaga cataggaccc
     1981 cagcccagat ctgaatggca tgggaggtgc tgcccttaac catgacacca ttgtaagagc
     2041 tgtccacatt tgtatgttgt gccctggaat cagcctggtt gagctcaaat cccaacttag
     2101 ccacgtctgg cctgtgtcct tgggcagtca cactacctct ctgattttgt ttccttatct
     2161 gtaaaatggt gatcatcata atacaacttc aaaaggattt caggctgagt gtggtggctc
     2221 acgcctatac acccagcact ttggaaggct gaggaaggag gatcgcttga ggccaggagt
     2281 ttgagactag cctaggcaac acagtgaggc cttatctcaa caacaaccac aaaatctaaa
     2341 aattagctgg gtgtggtggt gcatgcctgt gatcctggct acttcagagg ctgaggtgga
     2401 aggatcactt gaggccagga gtttgaggct gcagtgagtt atgatggcac tgctgcactc
     2461 cagcctgcgg gacagagtga gaccctgtct gaaagaaaga gagaaagaaa gaaagaaaga
     2521 gagagaaaga aagaaagaaa gaaagggaaa gatggaagga aggaaggaaa aagaaagaga
     2581 aagagagaga gagagaaaga aaaagaaaga aaagtaagaa agaaaaaaga aaaagaaaga
     2641 aaagtaagaa agaaaaaaag aaaaagaaag aaagtaagtc ctggctaaca cggtgaaacc
     2701 ctgtctctac taaaaataca aaaaattagc caggcgaggt ggcaggcgcc tgtagtccca
     2761 gctactcggg aggctgaggc aggagaatgg cgtgaacccc ggtgggggcg gagcctgcaa
     2821 tgagccgaga tcgcgccact gcatccaacc tgggtgacag caagactccg tctcaaaaaa
     2881 aaaaaagaaa gaaagaaaag aaaagaagga aagaagcggg gcacagtggc tcagtcctgt
     2941 aatcccagca ccttgggaag gccaaggtgg gaagatcact tgaggctagg agtttgagac
     3001 aagcctggcc aacatagcaa gaccccatct ctacaaaaat aaaaatttta aaaagggctg
     3061 gggcatttga gctgggtccc aacagtagac aagtagaaaa ggcatggaga gggcatacca
     3121 ggtgggagga gctgtgtgca aaggcctgga gatggaaaag catgctggcc accagcttct
     3181 gacaagcagt ttagtatgaa cggtatgcag ggaaagaggg aaggagggca gaggggtgcg
     3241 cacgagcacc cgttagtgtc cttaaatgac cagcatggga acctggtctc tttcctgagg
     3301 gccctagaga gccatggaag ggtttagagt aggagtgggt catggttagg tttgggtttt
     3361 aggatgatcc ccctagttgc tctggagagg tggagcagag cgggtactga ggctgggtgc
     3421 ctgtgggagg ctgaggcagg aatgaagagg gagacgatgg gcctgggctg agccatggaa
     3481 gggaggaggg gtgagtgcgg gcacactcag gagccaagtg gacaggccat agcccccaca
     3541 gactggaggg acgcggctag ggaatgtccc acagagtggc cagttatccc tgagagaaag
     3601 agcaggtttt agcggagacg ctgaggctgc tttagaatat ggtgggtgtg tggggcaaaa
     3661 gggacaccca ggggtgtatc aagaggtcat agggggcagg ccaggtggct cacgcctgta
     3721 atcccagcac tttgtgaggc cgaggcaggt ggatcacctg aggtcaggag ttcaagacca
     3781 gcctggccaa catggtgaaa ccccgtctct actaaaaata caaaaattag ctgggtgtgg
     3841 tggcacatgc ctgtagtccc agctacttgg gaggctgaga cagaattgct tgaacctggg
     3901 aggtggcggc tgcagtgggc cgagatcaca ccactgtact ccagcctggg cgaaacagag
     3961 actctgtctc aaaaaaaaaa aaaaagtcat aggggctggg catagtggct ctcacctata
     4021 atcctagcaa atcgtagtac tttgggaggc cgaggcagga ggattgcctg agcccagggg
     4081 ttcaagacca gcctcagcaa cataaaatac aaaaaataca aaaattagct gggtttggtg
     4141 gtgtgcacct gtggtcccag ctactcagga ggctgaagtg ggaggatcac ttgagcctgg
     4201 gaggtcaagg ctgcagtgag ctgtgattgc atcactgtac tccagcttgg gtgacagagc
     4261 aagaccctgt ctcaaaaaaa aaaaaagaaa agaaaaagaa aaagaaaaag aaaatatgta
     4321 tattggatat actatgtgcc aggcacgatt ccaagcccct gatacattct ctacatttta
     4381 cagatggggg aaggaggtcc agagatgtta aatacttgcc caaggtcaca cagaaagtgg
     4441 aacactggga tttgaacaag gttttggttg ggcatctttt cctatgggag ctcagaaata
     4501 tctgttgtct agccctttct cagcctccca accttctcgg ttccttacct atgtcacagc
     4561 tgactttgag ctaaagtcat ctcggggcag ctaggtgcct atgtgagctg gcgttcattt
     4621 ctcactgttt ctccttccaa atacctccag gaagaaaagc aagtcctttt tggtccaaga
     4681 cattaaaaaa gccaggctca gcggctcatg cctgtaatcc caacacttgg ggaggctgag
     4741 gcaggaggat cgcttgagcc cgggagctgg agaccagcct ggacatgtta acatagcaag
     4801 acccctctac agaaaatgtt aaaaatgagc tgggtgtggt ggcgcacacc tgtagtccca
     4861 gttactcagg aggatgaggc aggaggatca cttgagccta ggaggttgag gctgcagtga
     4921 gccatgatca cactactgga ctccagcctg ggccatagag caagaccctg tcctaggaga
     4981 aaaaaaaggg ccaggcacag tggctcacgc ctgtaattcc agtactttgg gaggccgagg
     5041 cgggcggatc acaaggtcag gagatcaaga ccatcctggc taacacagtg aaacccgtct
     5101 ctactaaaaa tacaaaaaaa ttagctgggt gcggtggcac gcacctgtga tcccagctac
     5161 tcgggaggct gaggcaggag aatcacttga acccaggagg tggaggttgc agtgagccga
     5221 gatcccacca ttgcactcca gcctgggaga cagagcgaga ctccgtctca gaaaaaaaaa
     5281 aaaaagaaaa aagtgtgtgc taaaaaattc atcaggtact aaagagtgta cggtggaaag
     5341 taagtctttc tcctacactc agatcatgga tggatgtctc aggtcttccc aggcacattt
     5401 gatgttatgt accagccata tgcatgtgtg tgtcatttat ttttcactct acacaagtag
     5461 tacctaatat ggtgaggttg ttatgattac agactctgga ggcaaagttc ctgggtataa
     5521 atctgaactc tgtcactccc aagctaagtg gctttgggca agtgatttca cctccatgtg
     5581 cctcagtttg ctagtctgta caatgggatg gtaatagcac ctacctcatg gacttgagaa
     5641 gtcagcaact tcatacctct aaagccctta gagctagcag tgtgtggcac tcaataaaat
     5701 gttgactaat gatgattctc a
//
