LOCUS       NM_022055              13564 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens potassium two pore domain channel subfamily K member
            12 (KCNK12), mRNA.
ACCESSION   NM_022055
VERSION     NM_022055.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Tateyama,M. and Kubo,Y.
  TITLE     Regulation of the two-pore domain potassium channel, THIK-1 and
            THIK-2, by G protein coupled receptors
  JOURNAL   PLoS One 18 (4), e0284962 (2023)
   PUBMED   37099539
  REMARK    GeneRIF: Regulation of the two-pore domain potassium channel,
            THIK-1 and THIK-2, by G protein coupled receptors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   Blin,S., Chatelain,F.C., Feliciangeli,S., Kang,D., Lesage,F. and
            Bichet,D.
  TITLE     Tandem pore domain halothane-inhibited K+ channel subunits THIK1
            and THIK2 assemble and form active channels
  JOURNAL   J Biol Chem 289 (41), 28202-28212 (2014)
   PUBMED   25148687
  REMARK    GeneRIF: In cell and tissues co-expressing THIK1 and THIK2,
            heterodimeric channels may contribute to cell excitability.
REFERENCE   3  (bases 1 to 13564)
  AUTHORS   Chatelain,F.C., Bichet,D., Feliciangeli,S., Larroque,M.M.,
            Braud,V.M., Douguet,D. and Lesage,F.
  TITLE     Silencing of the tandem pore domain halothane-inhibited K+ channel
            2 (THIK2) relies on combined intracellular retention and low
            intrinsic activity at the plasma membrane
  JOURNAL   J Biol Chem 288 (49), 35081-35092 (2013)
   PUBMED   24163367
  REMARK    GeneRIF: The cytoplasmic amino-terminal region of THIK2 (Nt-THIK2)
            contains an arginine-rich motif (RRSRRR) that acts as a
            retention/retrieval signal.
REFERENCE   4  (bases 1 to 13564)
  AUTHORS   Birlea,S.A., Gowan,K., Fain,P.R. and Spritz,R.A.
  TITLE     Genome-wide association study of generalized vitiligo in an
            isolated European founder population identifies SMOC2, in close
            proximity to IDDM8
  JOURNAL   J Invest Dermatol 130 (3), 798-803 (2010)
   PUBMED   19890347
REFERENCE   5  (bases 1 to 13564)
  AUTHORS   Nyholt,D.R., LaForge,K.S., Kallela,M., Alakurtti,K., Anttila,V.,
            Farkkila,M., Hamalainen,E., Kaprio,J., Kaunisto,M.A., Heath,A.C.,
            Montgomery,G.W., Gobel,H., Todt,U., Ferrari,M.D., Launer,L.J.,
            Frants,R.R., Terwindt,G.M., de Vries,B., Verschuren,W.M., Brand,J.,
            Freilinger,T., Pfaffenrath,V., Straube,A., Ballinger,D.G., Zhan,Y.,
            Daly,M.J., Cox,D.R., Dichgans,M., van den Maagdenberg,A.M.,
            Kubisch,C., Martin,N.G., Wessman,M., Peltonen,L. and Palotie,A.
  TITLE     A high-density association screen of 155 ion transport genes for
            involvement with common migraine
  JOURNAL   Hum Mol Genet 17 (21), 3318-3331 (2008)
   PUBMED   18676988
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   6  (bases 1 to 13564)
  AUTHORS   Theilig,F., Goranova,I., Hirsch,J.R., Wieske,M., Unsal,S.,
            Bachmann,S., Veh,R.W. and Derst,C.
  TITLE     Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1)
            K channels in the mammalian kidney
  JOURNAL   Cell Physiol Biochem 21 (1-3), 63-74 (2008)
   PUBMED   18209473
  REMARK    GeneRIF: THIK-1 and THIK-2 are abundantly expressed in the proximal
            and distal nephron of the mammalian kidney.
REFERENCE   7  (bases 1 to 13564)
  AUTHORS   Girard,C., Duprat,F., Terrenoire,C., Tinel,N., Fosset,M., Romey,G.,
            Lazdunski,M. and Lesage,F.
  TITLE     Genomic and functional characteristics of novel human pancreatic 2P
            domain K(+) channels
  JOURNAL   Biochem Biophys Res Commun 282 (1), 249-256 (2001)
   PUBMED   11263999
REFERENCE   8  (bases 1 to 13564)
  AUTHORS   Rajan,S., Wischmeyer,E., Karschin,C., Preisig-Muller,R.,
            Grzeschik,K.H., Daut,J., Karschin,A. and Derst,C.
  TITLE     THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+
            channels
  JOURNAL   J Biol Chem 276 (10), 7302-7311 (2001)
   PUBMED   11060316
REFERENCE   9  (bases 1 to 13564)
  AUTHORS   Goldstein,S.A., Bockenhauer,D., O'Kelly,I. and Zilberberg,N.
  TITLE     Potassium leak channels and the KCNK family of two-P-domain
            subunits
  JOURNAL   Nat Rev Neurosci 2 (3), 175-184 (2001)
   PUBMED   11256078
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC138655.1.
            
            On Jul 23, 2019 this sequence version replaced NM_022055.1.
            
            Summary: This gene encodes one of the members of the superfamily of
            potassium channel proteins containing two pore-forming P domains.
            The product of this gene has not been shown to be a functional
            channel, however, it may require other non-pore-forming proteins
            for activity. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF287302.1, BC047749.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000327876.5/ ENSP00000327611.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1045              AC138655.1         94943-95987         c
            1046-13564          AC138655.1         34292-46810         c
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.3"
     gene            1..13564
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="potassium two pore domain channel subfamily K
                     member 12"
                     /db_xref="GeneID:56660"
                     /db_xref="HGNC:HGNC:6274"
                     /db_xref="MIM:607366"
     exon            1..1045
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             655..1947
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="tandem pore domain potassium channel THIK-2;
                     potassium channel, subfamily K, member 12; tandem pore
                     domain halothane-inhibited potassium channel 2; potassium
                     channel, two pore domain subfamily K, member 12"
                     /codon_start=1
                     /product="potassium channel subfamily K member 12"
                     /protein_id="NP_071338.1"
                     /db_xref="CCDS:CCDS1835.1"
                     /db_xref="GeneID:56660"
                     /db_xref="HGNC:HGNC:6274"
                     /db_xref="MIM:607366"
                     /translation="MSSRSPRPPPRRSRRRLPRPSCCCCCCRRSHLNEDTGRFVLLAA
                     LIGLYLVAGATVFSALESPGEAEARARWGATLRNFSAAHGVAEPELRAFLRHYEAALA
                     AGVRADALRPRWDFPGAFYFVGTVVSTIGFGMTTPATVGGKAFLIAYGLFGCAGTILF
                     FNLFLERIISLLAFIMRACRERQLRRSGLLPATFRRGSALSEADSLAGWKPSVYHVLL
                     ILGLFAVLLSCCASAMYTSVEGWDYVDSLYFCFVTFSTIGFGDLVSSQHAAYRNQGLY
                     RLGNFLFILLGVCCIYSLFNVISILIKQVLNWMLRKLSCRCCARCCPAPGAPLARRNA
                     ITPGSRLRRRLAALGADPAARDSDAEGRRLSGELISMRDLTASNKVSLALLQKQLSET
                     ANGYPRSVCVNTRQNGFSGGVGALGIMNNRLAETSASR"
     exon            1046..13564
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2059..2064
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="hexamer: AACAAA"
     polyA_site      2083
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
     regulatory      6256..6261
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="hexamer: AATAAA"
     polyA_site      6278
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="major polyA site"
     regulatory      13538..13543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="hexamer: AATAAA"
     polyA_site      13564
                     /gene="KCNK12"
                     /gene_synonym="K2p12.1; THIK-2; THIK2"
                     /note="major polyA site"
ORIGIN      
        1 agtgactccg cctctccctg ccgggcggct cttcggctgg agctgagaaa ggagcgcttc
       61 cccggactcg gctcggctcc gaggctctga agccgacgcc gccagctcag ccccgggggc
      121 gggagcagga ctgcccgcac agcccgcacc taggaggcgc cgatcccgaa cgcctcatgg
      181 gacgcccccg ggggctctct ccacgccttg ctgccgcgtc ccggtcctag gcgcccggga
      241 tccacggccc accccgcccg cagcccgcgg cctgtctgga gaggagtatg aggcccgggg
      301 ccccgcggac gccggccacc gggcggcagg ggcctagctg cggagccccg cgcccgagag
      361 cggcgggtaa ggagccgcgg gagccggcga ggcgtcgggg cgcgcagagg agcgcccctg
      421 cccgggcacc cgctgggcca cgggactcgc gtgtggcctg agcgccgggg aggaggcgga
      481 ggcgcccctc tgtccgggct ctgggaaggc gacgaggggc tctgcgaagg cggcgagggg
      541 ctccgcggcg gccccggacc cctggccacc atcctcacgc tcctgctccc gccgggggga
      601 tgtcgtggcc cgggccccga gcgccgcccc ggccccgggg ctgagctccg gaccatgtcc
      661 tcccgcagcc cccggccccc gccccgccgt agccgccgcc gcctgccgcg cccctcctgc
      721 tgctgctgct gctgccgccg ttcgcacctc aacgaggaca ccggccgctt cgtgctgctg
      781 gcggcgctca tcggcctcta cctggtggcg ggtgccacag tcttctcggc gctcgagagc
      841 cccggcgagg cggaggcgcg ggcgcgctgg ggcgccacgc tgcgcaactt cagcgctgcg
      901 cacggcgtgg ccgagccaga gctgcgcgcc ttcctccggc actacgaggc cgcgctggcc
      961 gccggcgtcc gcgccgacgc gctgcgcccg cgctgggact tccccggcgc cttctacttc
     1021 gtgggcaccg tggtgtcaac cataggtttc ggcatgacca cccccgcgac ggtgggcggg
     1081 aaggccttcc tcatcgccta cgggctgttc ggctgcgctg ggaccatcct gttcttcaac
     1141 ctcttcctgg agcgcatcat ctcgctgctg gccttcatca tgcgcgcctg ccgggagcgc
     1201 cagctgcgcc gcagcggcct gctgcccgcc accttccgcc gcggctccgc gctctcggag
     1261 gccgacagcc tggcgggctg gaagccctcg gtgtaccacg tgctgctcat cctgggcctg
     1321 ttcgccgtgc tgctgtcctg ctgcgcctcg gccatgtaca ccagcgtgga gggctgggac
     1381 tacgtggact cgctctactt ctgcttcgtc accttcagca ccatcggctt cggggacctg
     1441 gtgagcagcc agcacgccgc ctaccggaac caggggctct accgcctggg caacttcctc
     1501 ttcatcctgc tcggcgtgtg ctgcatttac tcgctcttca acgtcatctc catcctcatc
     1561 aagcaggtgc tcaactggat gctgcgcaag ctgagctgcc gctgctgcgc gcgctgctgc
     1621 ccggctcctg gcgcgcccct ggcccggcgc aatgccatca ccccaggctc ccggctgcgc
     1681 cgccgcctgg ccgcgctcgg tgccgacccc gcggcccgcg acagcgacgc cgagggccgc
     1741 cgcctctcgg gcgagctcat ctccatgcgc gacctcacgg cctccaacaa ggtgtcgctg
     1801 gcgctgctgc agaagcagct gtcggagacg gccaacggct acccgcgcag cgtgtgcgtc
     1861 aacacgcgcc agaacggctt ctcgggcggc gtgggcgcgc tgggcatcat gaacaaccgg
     1921 ctggccgaga ccagcgcctc caggtagacc gcccgtccgc ccgcgccggg gaccctctcc
     1981 aggccgcggg gccgccgggc gtggtttgct tctctcagtc actgctggcg ctttcttaat
     2041 ctttatccaa taaaatgaaa caaaaaaaat ttttttaaag aaatactatt tggccaggcc
     2101 tgatgttatc aagggcaggt tttggggacg cctgtgttcc ttgtgagtcc cctcttggag
     2161 aaccgtggcc cccagcagat tctccttcag ggagagaaaa cgtggccccc caggcccaca
     2221 cccagggcat accgcagcga ggagggtttt gcagactggc actaaacccc gtccccatgc
     2281 acagaagcag ccggcaaccc caaagcatat ggtgcaactt ttcgtggctc tgaattacct
     2341 cctcagcatt tagaatcctg gcccagccta gcagcttcct tctggtcgtc agaagtgata
     2401 tagtcacagt tttgacaggt gggggttggg acagccatat gttgcctccc gatgtatata
     2461 tatagaacag ccactacaca cagagaatgg tgtagtatta tgcaatgaga tgaaacatag
     2521 caccaacttg cggactgtgg catttccccc agatttggaa attgcagtgg tgaggggctt
     2581 agctggcctt tctcagctca gagttagtcc atttagcaca gagcacaggg cagctacttc
     2641 taatggtggg cggtccaggg cacctgtgag gaggggagcg gtgaggctgc aggagccctg
     2701 ctgggctttt agctggctga tctccaggct cggatcggca gatcttgaat cccccaaccg
     2761 tcctttcttt cacaagctca tccacagcag tgcttttcaa tattaaccag gaatatctga
     2821 gtcttctcag acaagatatt tatttgttgg tgtggatttt ttttttctta actactgttg
     2881 atcagttcaa cttttccccc tttaagagat catggggttg ccgggcgcag ccctccagct
     2941 tctcctcctc ctcctctgaa ggaggcacct ctgccagctg cgtccttggg aaccagggcc
     3001 tgcagcacca ggaacccaaa agaagccatc ttgcaaatgt cagggtattt ggctccagag
     3061 gccatgatgt gtttggggtc aggaaagata gaatcccttt ctaaatattg tttatcaaga
     3121 ggatttgtct tctccatgca cagcctattg gaggcagagg aaggcaggag ggaggtagct
     3181 ggtgaaactg gtggatggaa gtaatgttag gattacagtt ttgctctcgg gctgagggac
     3241 agattctccc agacttgcct tctgcagcat agggggtccc tgacctggag gaaggccaga
     3301 tacccaggct tcattgaaag gatagtggtc tgggggtgta aacgaacact ctctctgccc
     3361 atttttctca tatatgatga gggcaaggac aggtgccaga gtttatacag gcacggcgtg
     3421 acccaccagt atgcacagct tctgatagga agtcttagaa aagcaccgcc tcaccgcctc
     3481 cagctggagc catgctgtga aatctctctc cctggcaaat gtgcgtggaa cacacacaca
     3541 cacaaacatg cacgcatgcg ctcacgcacc cagcattaga gtcaaacttg aatgtcaaac
     3601 tcttctttcc ccttctagtt catggcagga agggaaagct tacctgagat ctcagtgact
     3661 ctccagctat tttgggtaat ttctgatttg agttgggatt ggtttttaaa cacaggccct
     3721 aatctggggt gaagccagtg aagcagcgct ggccaggaga gacgtcagtc ttgctaaatc
     3781 ctgaaggtgg gttcctgtga aaaccatgct ttcaggattc actgtgactg tcaggccagc
     3841 ccagtgagtg ccccccatcc aacccatggt tgcggtcaca gtgaagggat tgcccacgca
     3901 ggggcgcacg tgttctctag ggcagggccc tctgacagga cctggaaata aaatccacat
     3961 ttatcccttt aaaaaaggga acgattaaaa ggcatccaat tagaggtgtt acctaggcaa
     4021 caggaaataa cttggggatc agagccatcc agagagggaa gaagagatcc ccatgataga
     4081 ggcatctaga cagtgaggac ttgatctgcg ggcaaaataa cagcctaccc acaggaagtg
     4141 agccccagca acagtacagc agcctaaaac ccaggcatca agactgtgtg agctagacag
     4201 gtctaaagaa acctccaggc aatcccccgc ccagcgtgca ggactacaca gaatcgtggt
     4261 ctccatggga ggaaccagga cccacctcca gagaagaaaa actcaaaatg tttcttggca
     4321 gtcttctctg ttggcggtgc ttcctagact ctatcctaag ttgcttatgc tgcagcctca
     4381 gctgttttta cttgttttcc tatgctttgg ttttggaaag aggcaattgg aggcagcttg
     4441 agcaatcttc taaaggaatg gtaagcatgg tttcaagggg caggaaaatg agacccttcc
     4501 tcttcccacc tcccaacttc ccagagaaac gccagtcctc caaggtacaa tggcagctca
     4561 ggatccagtg gcacatgtgt atttttgcct atgttcccct gtccccatcc cccaatccag
     4621 tctcagtcac actgccatga tacatcacac catcatcctt catctcaggc caaagggctc
     4681 agcgccccag acgacaggga ctgcttccct gaagcaggga tgtggttctc ccatcatttc
     4741 aggaacagtg acattgatag ggatgccttt ctttggtgca ggttcttctg aacctcaggc
     4801 caagaaagag ctccattccc tcagatgctg tggcctctct cactgtcttg ggggtctcca
     4861 gtttgcacac cactggtagc tcactgtagc ctacaatttc ctcatgctcc aggatttgct
     4921 tcctaacctt aggcctggac ttacccatca tgccggtgct gtggggctcc cttaaagttt
     4981 gaatgaactc caagaggccc tcaccatatg actgtggcca cagagaatcc agagtcttgg
     5041 ctagcagcct tctcagagct gaggagaaag gtaaagaagc caccctgggt gtcctagctt
     5101 gctaaagcca ggtggtcagg cctttgtgtc ctctgaatga agcagtgcct ccaggcctgg
     5161 agaaggcatc agaaggctta ggcaatctca ggctctcatt ttgtggaaaa tgccagacag
     5221 ccctgggcat ttgcacagca tcatgcatat atgtatatat atatacacac acacatacac
     5281 accagcctta caaagagcta ctaacccttt tccccaagga aaagtctcag tctgggccac
     5341 tttaagaaga aagatttccc atttttaact ctctagagat gtaccagggc ctggaaaatc
     5401 cctctctgcc ttgggggagt tttatgtaca atttgcatgt attcaggttt cacacagaag
     5461 ccagcatcca aacccaagcc aaaccctgga aaatagctgc taaattgacc caggatcctc
     5521 cgtccttcag acaggcctcc cagcagaccc cctcttcctg tggactctgg ggtgagggat
     5581 gactgagccc tcatggccaa aggagaagct tctggggaac tgaggcagac ggcagcccag
     5641 tcaggcagag accataggag cgacgggccc tgtggttcga tcttactgta actttttaca
     5701 ttcttaaaaa acaaaaagca caattccaca tacacactgt ctggaaagca cagccagaca
     5761 gccagcctag ggcatctgag tccagaagag gcgctggggc tttatctgtt gcagggttct
     5821 tgtattgttg tataatgtgc tggtggtgac tcaaggctcc tcactttgta gggtggagag
     5881 ggagggaggg agggcctgct gaggccattc ctgaccccca gggtgggctc atcagcatgg
     5941 acttggggcc tggactcttg cccctgaagc agctctggca ctttcaggca ccccagagca
     6001 caattgctac accttccacg ctagtctcat gcattgagga tttctgcact cggacctggc
     6061 cccaaaccct cttccccttc cccttcttcc ctaaggaagc tcccccttgc tttacaaatc
     6121 attttttatg ctatctgttc tctcaggagg gggctagagc atgcgggaca agcctgcagg
     6181 gtttctcaaa caatacatct atttttctga gcagcattcc aagagaggac ttgaagggtg
     6241 tttaaattaa cgatgaataa aatgcagcct gccacccctc tgagctgttt gctttctgtc
     6301 tgtcagagcc gcctgcctcc ccatcttcca gttcacacct cctctcagga gggcccgggg
     6361 gacttcactg ttaggatggg gcccagtggc agcatcactg ggaagttgct ctacaaaaca
     6421 acagtgaaac agagcccagg gcaccaaggg acctcctggg cagcttggcc aggctgagcc
     6481 aaggctgggg gactgggaga tggatccaac ctggctgtgc ccgaggctct ccaaatggag
     6541 ctttaaagga tcccgaggcc cgggccgtac tcccaggagt ctgatttcat tggtctaagg
     6601 agcaactcag gccagtcctg gaattttttg gggggattct aatgggcagt catggacttg
     6661 aatacaccct gtaaacacag aacactcaca ttatggtggt gtagaaagag cgtccactta
     6721 gaaactagga ggtctgaatt ctagttttgg atctggcagt catttagctc actctgatcc
     6781 tgtttcctca tctctaaagt gggatgatga taccatcccc ccacctccca gcggtcttac
     6841 gagaaataag tgaggggcta ggaactccga aaagtgtaaa gtcctccaca attgcaagat
     6901 gtcacaatta taagaggcat ttcatgagga tatgggacca ggctgcccta ttgcttctgc
     6961 atggggtcct tcccacagtg tcagctgggg atgaagttct tccagcagat tgcttttgcc
     7021 tgggttggct ttgcaaaccc cttcttttca gaagcagctt acttgcccta ataatagtgc
     7081 aaactctact gaggccttct ctcccctcca catccccctc cttcttttcc ttcaattttc
     7141 cctagaaatg agaagtaatt gaccatcagg ctcacactgg ccccacccac ctctgtctat
     7201 acgatcactc cccactcccc tgggctcccc accctgttga gcatgaccac tcagaagcac
     7261 aagctccatc ttttatctac agcagggact ttcatgacat caaatgataa aacaagaagt
     7321 tttctacaac tatactgtac agatcatgca ctattttcca ttctggtatt gttttgtttt
     7381 gtcttgtttt aacccattta tgctggaggt tgcaaatttt ttgtgtaaaa aatcagattt
     7441 tggcgatgac cttaagcagt aggatgtaaa taactctcac agacttagtg ttccaataag
     7501 ggaacgctag gcataaatag gttaatgctg gtcaaagcta ttaaactgat ttcatgatct
     7561 gcctatgaat tacacccagc aatctgagaa acactggctt acagtgtttc tggaaagagg
     7621 ccttcctgtt agcttccagc caagacccat tagctaggct gccgtggcgg agaggcctga
     7681 tttctcgtca cccatcagct ctcctctctg ttggggtctc cagctgtgcc ccagctatgt
     7741 gatgggcaca ggtggctgtt ttacctgcat aaagtagcct agatgatcct tgggttcctt
     7801 tcaagttcta aggcctgtga ttcctataac atttcatatg acactccagt gatccctgga
     7861 tggactgcca ttcctctccc ttctgtggcc agttaccttt ctatgagtgt atctaacaag
     7921 cagattttaa aaaggaactt ttagctgggc atggtggctc atgtctataa tcccagcact
     7981 ttgggaggct gagacaagtg gatcacttta gcctaggagt tcgagaccag cctaggcaac
     8041 atgtcgaaac cctgtcttta ctaaaaatac aaaaaattag ccagtcatgg tggtgcacgc
     8101 ctggagtcca agagactcag ggggctgagg tgggaggatc gcctgagccc aggaagtcga
     8161 tgtttcattg agccatgatc atgccactgc actccagcct tggtgacaga gtaagaccct
     8221 gtctcaaaaa gaaagaaaaa aaaaaaagaa aaaggaagaa aagaaaaaag aactttatat
     8281 ttaccaagcc agacccccag ataagagact tgcatattta tcttatttaa tcccctggcc
     8341 aatcttggaa atttggcact ttcctcccat tttgtcttgg gtcagagagg tcaagtaact
     8401 cgtctagttt ggggtcaggc atcccctgtg ttaggggctg gagagacaag ggtgagaaag
     8461 acaggaccgc ccttgatgtc cctggactcc aggctcctgg aggctgacgg gggcagggcg
     8521 ctgcagtacg gcctgctgag ggctccctgt ccaccaggca ttctgggctg ccatgggcac
     8581 accaaagaga ggcagccacc cagcctgggt aggcagagaa ggctctcaga agcagctgtc
     8641 catgctgagc ctgagagcag caagcctgct gacaaggagc aggaagagtg gcccaggcag
     8701 aggagcagtg tgtgggaaga acagagcagg gccttgttgg gggaactgtg tgtagttctg
     8761 caggactgga gcatgcagag caagggaggt aatgaggggg ccagatagcg aagggccttg
     8821 taaaacaaag caaagacttt tcgcctaatc ctgaggacac tggcaagcca gtggaggggt
     8881 tttaaggatg cgtgtgctgc cgaaaatgac tcagaatgca ggagtggagg aggaaagcag
     8941 atggcagggt ggaccaaggt agtgctagtg agaacacaga gacgtggaga gatttgaggg
     9001 acacccgaaa gagtaggtcc acagaatcaa tgatttgcct ggatggggat gataagactg
     9061 agagagagga attgggacgg agccaggtct caggctaagc agccaggtag acagtggtgc
     9121 caccactgag cctgggaacc caggaggagg aacagaagag gtgatgagtt caatgaagga
     9181 gaagttggac ttcagggacc catgagataa ccatggggga ggggttccca ccaagcaatt
     9241 ggccttgtga gtctagagct cataacaatg acccaggcta gaccaacatt ttggaagttc
     9301 tcagcatcaa gatggtgtga attactcagg ctgtgagtgt gtagaatgag ataaaaagag
     9361 gatcacaacc agcatttaag ggacaggagg aagaagaaaa aagactaagg aaagagggtc
     9421 agagatatgg gagaaaacca agagcacagg atgccacaaa gccaagggaa acaagggttt
     9481 caagaaggaa taagtgccaa gtgccactga gtgattaaga caaggactga agaaaacacc
     9541 cacaagattt agcaaaggga aatatcagtg accttggcag aagcagccgc aggggagtgt
     9601 gagcttcttt ggaactggtg ctgcctggct aattttcatg ggtcacacat tagggataca
     9661 agttaggata cagattcaag ccactctaac agaggttcaa aataatactg gcttccgttg
     9721 gtaaaagttc atgtctctct cttaagagtg agtgcaagca taatctatgt agtatcagac
     9781 acttctatct gtacctatca cttgtgttcc cttgccactg gccagaactt tgtcacatga
     9841 cctaatatag ctgcaagata atcaggaaaa gtggccatga accaagcaag gctggtactc
     9901 ccaagaagag tgttgttcat ctcatgttca ggccactgag gggacattat gcagctgcag
     9961 tgggacctct gaggggtgac aatgagggta gtatgatagc tacctgttga tagtggattg
    10021 atttagactt cagctggaaa caggaagtga gtcccttcct ctttcctccc tcttcctgcc
    10081 ctccccaacc aacaggctct cccagtggta aacctgtttg gaaggaatcc aacaaggagt
    10141 tctgggaaat gtagtttgct cagcaatgca gagcagagtt cagaggcagt gggtgtacaa
    10201 gggcacatca tcccattagc acactttagc cattgtgaag ttcccagaag gcgctttctc
    10261 actgtaagtc ctggaatctg ggcagagaaa gttatattcc tgaccccttt ctggtccatt
    10321 tttgagagct tcctgctcca ttttgaggga aatgtttaca gaagggatgt ttgtagaatt
    10381 acaaatgaaa aacttaacca gaaggcaaca gacaccaggt catgagagct caccactgcc
    10441 ctggttctgt ctctctggcc gactgcaagc agatctcttt cccccacgaa gtttcctttt
    10501 ccatgtcagc aaaatgaggc actggtcttt tggctctgtc actctgaggt tctgaaagga
    10561 aggagaagag aggagaaaat gtaggagttc cacggggatt tccatcaaga acgatactca
    10621 gcaaacatct gcagagtacc tgacccagct tagacagttt gttcaattct tttttctcca
    10681 tggactggga ggagcaggac cagccatctg gatgcaggat ggaaagaaat gtcctccact
    10741 aaggactggc caggttcctg ctgaaggtgc agcttcaaca cacatacccc accccatgag
    10801 aaacttcatg agctccagat cctcgttttc atttgtttct gcttccccaa ggacaagtaa
    10861 agtaccattc tccagaactg cacagtccaa taggcagcca caagccacac acagctattt
    10921 aaatgtaagt gaatttaaac tacattttca gctcgtcaat tgcactaacc ccatttcaag
    10981 tgttcaatag ccacatgtaa cttgtggcta ccatattgga tagcacagat atagaacatt
    11041 tctgtcactg cagaaaagtc tacaacgcag ggctgctcta atgagagctc tgggatccga
    11101 aagcctgaat tccagggcat ccttaagggg cagaagtaaa aggccttccc caaccatcat
    11161 gccccactgt ggtctcctct gcctccaagt ggccttgtct tcctcatccc ctatggaaag
    11221 acccccacct gctgttcacc gtgcacccat ccctctccca cccccagggc aaaagcattt
    11281 ggttaccctg tgatgtcaca cctgtgcggg taacgtcatg cactgtgggc atctctctgt
    11341 aatatggaag cccctcccac cacagacacc acagatgctg gggttctgga cagatttctt
    11401 tagaatacat gtatttgcac acatgagagc attggtccaa atacatatat gcaggcgcag
    11461 gcacacaaat atttatatta aaagcctaat tccacaaatg gctgtaatta agacacaaag
    11521 caaaacctca tgaggttgct cccttcaact cacacattta ctctgccatg ctctggcagc
    11581 aggggaacaa aaaatggaag atttgcttga ctctgcagac agagggaaat gaggctcagt
    11641 aaggaggcca cacagcaatt cactagctaa ttcaagcaaa agccagatat ccttcctgct
    11701 agcccacagc tctgtcctgt ccattcgttt ggtattgtat tcattcactc aacaaacata
    11761 tgtgagaacc tacggtgtgc atgctctgta ctgggccaca cagagattac acagtcaatg
    11821 agacatgaat ctggccctct atggtctagg agctaggaca tgttggttgc caactataga
    11881 agcagggata tcacagtcat accaccaggg gcaggctcag agaagtcccg gagaggctgc
    11941 ttcagatctg agtcccatgc caagcactag gctgctctgt gcttcaccca gctgtacttc
    12001 tccggccctt cattcctttt ctatcttcaa cctggcaaag aataagctat tcttcattta
    12061 ttctgggcag ccagttaata ttaataccag ggttaataga cctgtttctc ctgtacacca
    12121 tcttgtctgc tcctctacct aaaattgccc taagataagt tctaaaaatc cttcttctcg
    12181 tgggcaggtc acagtgctgc tgtgttccac tgaatagaga taaacgacga gagttggtca
    12241 tattcatagc tgaccaaata cgtgcggtga gagtgtaggg gtgttgagat agcccccagg
    12301 gcagatcctg gagccttgac ttttgtgtac ccaggatgct ttcctgtccc cttcttctag
    12361 ggacactatc tctgttcttt tagataactc cacccatctc catgcagtct agtggaactg
    12421 ccaatcacag ggtccctcct gtatcagtta tgaaagcatt caactgtaat gaacagaaaa
    12481 cccattgaag ggtagtttta aggaataaaa atcaaggttt atttcattac cataaccaga
    12541 actctggagc aggtggtctg gggctagcgc agtgacagtg atgccatcgg taaggctggc
    12601 tcctccttcc cgctctgcca tcctttccat gttggcttgc caccccaggt gccagatggt
    12661 agctacccct ccaaggctca catccatgtt actagtagca agaagtagca gggagaaggt
    12721 aaaaaatgct ctctcccaac aggacttttc ctagacctcc ctaggacttt ctgctgcatc
    12781 tcattgacca gaagagagtc acatgatcgt caccacctat aagggaggga gggagagagt
    12841 gttgtgcttt cagtcctctg tacaagagga cagcaagggg gaggggattg ggatgggtgt
    12901 gaagtgagaa tgcctacagt tcctgctgcc tccccatcaa ggctggatta atcacggttc
    12961 cgcattctca gagaaacagc cattgatcta agggagggtg catgcctcag gttggggagt
    13021 gggggggaag tcccctggcc ttacacaaat tggagggggt ggagtagccc tttcttgtct
    13081 tggtttataa attaggcctg gcggcatgag tcagggtcaa gttaatgcaa tagaatccag
    13141 tccagctgat ttaagcagaa aaagacctat cattggcaat caactccaac ctggccacct
    13201 agctctctga cactgatgac ctgactgggt gacagaatcg tgactccagc acgtcatgcc
    13261 acagtcctcg gacagggtct tatgtgctaa ggagagacac ctggacacca caatcctgca
    13321 ccaggtacca tctgcacaca gcaggccacc tccagccagc taatgctgat acaactgacg
    13381 tgttggcagc cagctgacat cagagactgt ggtttcctgc tatgggacag agaatttgca
    13441 atctgttggt tttcaaagac ctaccccatt tcacctcccc catctcatcc tgaacaacct
    13501 tccaagaccc tggcctgaat caggtttcct gtttggcaat aaaacttttt cactcagtgc
    13561 tcaa
//
