LOCUS       NM_002240              19659 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens potassium inwardly rectifying channel subfamily J
            member 6 (KCNJ6), mRNA.
ACCESSION   NM_002240
VERSION     NM_002240.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 19659)
  AUTHORS   Lee,S.J., Maeda,S., Gao,J. and Nichols,C.G.
  TITLE     Oxidation Driven Reversal of PIP2-dependent Gating in GIRK2
            Channels
  JOURNAL   Function (Oxf) 4 (3), zqad016 (2023)
   PUBMED   37168492
  REMARK    GeneRIF: Oxidation Driven Reversal of PIP2-dependent Gating in
            GIRK2 Channels.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19659)
  AUTHORS   Ozberk,D., Haywood,A., Sutherland,H.G., Yu,C., Albury,C.L.,
            Zunk,M., George,R., Good,P., Griffiths,L.R., Hardy,J. and
            Haupt,L.M.
  TITLE     Association of KCNJ6 rs2070995 and methadone response for pain
            management in advanced cancer at end-of-life
  JOURNAL   Sci Rep 12 (1), 17422 (2022)
   PUBMED   36261449
  REMARK    GeneRIF: Association of KCNJ6 rs2070995 and methadone response for
            pain management in advanced cancer at end-of-life.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 19659)
  AUTHORS   Masotti,A., Uva,P., Davis-Keppen,L., Basel-Vanagaite,L., Cohen,L.,
            Pisaneschi,E., Celluzzi,A., Bencivenga,P., Fang,M., Tian,M., Xu,X.,
            Cappa,M. and Dallapiccola,B.
  TITLE     Keppen-Lubinsky syndrome is caused by mutations in the inwardly
            rectifying K+ channel encoded by KCNJ6
  JOURNAL   Am J Hum Genet 96 (2), 295-300 (2015)
   PUBMED   25620207
  REMARK    GeneRIF: Keppen-Lubinsky syndrome is caused by mutations in the
            inwardly rectifying K+ channel encoded by KCNJ6.
REFERENCE   4  (bases 1 to 19659)
  AUTHORS   Whorton,M.R. and MacKinnon,R.
  TITLE     X-ray structure of the mammalian GIRK2-betagamma G-protein complex
  JOURNAL   Nature 498 (7453), 190-197 (2013)
   PUBMED   23739333
  REMARK    GeneRIF: 3.5 A resolution crystal structure of the mammalian GIRK2
            channel in complex with betagamma G-protein subunits, the central
            signalling complex that links G-protein-coupled receptor
            stimulation to K(+) channel activity
REFERENCE   5  (bases 1 to 19659)
  AUTHORS   Finley,M., Arrabit,C., Fowler,C., Suen,K.F. and Slesinger,P.A.
  TITLE     betaL-betaM loop in the C-terminal domain of G protein-activated
            inwardly rectifying K(+) channels is important for G(betagamma)
            subunit activation
  JOURNAL   J Physiol 555 (Pt 3), 643-657 (2004)
   PUBMED   14724209
  REMARK    GeneRIF: L344 and G347 play an important functional role in
            G(betagamma) activation of GIRK2 channels.
REFERENCE   6  (bases 1 to 19659)
  AUTHORS   Ivanina,T., Rishal,I., Varon,D., Mullner,C.,
            Frohnwieser-Steinecke,B., Schreibmayer,W., Dessauer,C.W. and
            Dascal,N.
  TITLE     Mapping the Gbetagamma-binding sites in GIRK1 and GIRK2 subunits of
            the G protein-activated K+ channel
  JOURNAL   J Biol Chem 278 (31), 29174-29183 (2003)
   PUBMED   12743112
REFERENCE   7  (bases 1 to 19659)
  AUTHORS   Ferrer,J., Nichols,C.G., Makhina,E.N., Salkoff,L., Bernstein,J.,
            Gerhard,D., Wasson,J., Ramanadham,S. and Permutt,A.
  TITLE     Pancreatic islet cells express a family of inwardly rectifying K+
            channel subunits which interact to form G-protein-activated
            channels
  JOURNAL   J Biol Chem 270 (44), 26086-26091 (1995)
   PUBMED   7592809
REFERENCE   8  (bases 1 to 19659)
  AUTHORS   Sakura,H., Bond,C., Warren-Perry,M., Horsley,S., Kearney,L.,
            Tucker,S., Adelman,J., Turner,R. and Ashcroft,F.M.
  TITLE     Characterization and variation of a human
            inwardly-rectifying-K-channel gene (KCNJ6): a putative
            ATP-sensitive K-channel subunit
  JOURNAL   FEBS Lett 367 (2), 193-197 (1995)
   PUBMED   7796919
REFERENCE   9  (bases 1 to 19659)
  AUTHORS   Tsaur,M.L., Menzel,S., Lai,F.P., Espinosa,R. 3rd, Concannon,P.,
            Spielman,R.S., Hanis,C.L., Cox,N.J., Le Beau,M.M., German,M.S. et
            al.
  TITLE     Isolation of a cDNA clone encoding a KATP channel-like protein
            expressed in insulin-secreting cells, localization of the human
            gene to chromosome band 21q22.1, and linkage studies with NIDDM
  JOURNAL   Diabetes 44 (5), 592-596 (1995)
   PUBMED   7729621
REFERENCE   10 (bases 1 to 19659)
  AUTHORS   Lesage,F., Duprat,F., Fink,M., Guillemare,E., Coppola,T.,
            Lazdunski,M. and Hugnot,J.P.
  TITLE     Cloning provides evidence for a family of inward rectifier and
            G-protein coupled K+ channels in the brain
  JOURNAL   FEBS Lett 353 (1), 37-42 (1994)
   PUBMED   7926018
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001427.1, DR000377.1,
            D87327.1, BC101547.1, HY332424.1, AP001407.1, AP001419.2 and
            KF457262.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002240.4.
            
            Summary: This gene encodes a member of the G protein-coupled
            inwardly-rectifying potassium channel family of inward rectifier
            potassium channels. This type of potassium channel allows a greater
            flow of potassium into the cell than out of it. These proteins
            modulate many physiological processes, including heart rate in
            cardiac cells and circuit activity in neuronal cells, through
            G-protein coupled receptor stimulation. Mutations in this gene are
            associated with Keppen-Lubinsky Syndrome, a rare condition
            characterized by severe developmental delay, facial dysmorphism,
            and intellectual disability. [provided by RefSeq, Apr 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.254086.1,
                                           SRR3476690.362903.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMN03465402
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000609713.2/ ENSP00000477437.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AP001427.1         8856-8874           c
            20-98               DR000377.1         8-86
            99-1632             D87327.1           1-1534
            1633-1633           BC101547.1         1087-1087
            1634-2545           D87327.1           1536-2447
            2546-2809           HY332424.1         1-264               c
            2810-7096           AP001407.1         1-4287              c
            7097-12698          AP001419.2         13439-19040         c
            12699-13166         KF457262.1         1-468               c
            13167-19659         AP001419.2         6478-12970          c
FEATURES             Location/Qualifiers
     source          1..19659
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.13"
     gene            1..19659
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /note="potassium inwardly rectifying channel subfamily J
                     member 6"
                     /db_xref="GeneID:3763"
                     /db_xref="HGNC:HGNC:6267"
                     /db_xref="MIM:600877"
     exon            1..574
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /inference="alignment:Splign:2.1.0"
     exon            575..626
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /inference="alignment:Splign:2.1.0"
     CDS             602..1873
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /note="inward rectifier potassium channel KIR3.2; inward
                     rectifier K(+) channel Kir3.2; potassium voltage-gated
                     channel subfamily J member 6; potassium channel, inwardly
                     rectifying subfamily J, member 6"
                     /codon_start=1
                     /product="G protein-activated inward rectifier potassium
                     channel 2"
                     /protein_id="NP_002231.1"
                     /db_xref="CCDS:CCDS42927.1"
                     /db_xref="GeneID:3763"
                     /db_xref="HGNC:HGNC:6267"
                     /db_xref="MIM:600877"
                     /translation="MAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHI
                     SRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTV
                     TWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVI
                     TDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKL
                     CLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGDDRLFLVSP
                     LIISHEINQQSPFWEISKAQLPKEELEIVVILEGMVEATGMTCQARSSYITSEILWGY
                     RFTPVLTLEDGFYEVDYNSFHETYETSTPSLSAKELAELASRAELPLSWSVSSKLNQH
                     AELETEEEEKNLEEQTERNGDVANLENESKV"
     exon            627..1547
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..19659
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      19637..19642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /note="hexamer: AATAAA"
     polyA_site      19659
                     /gene="KCNJ6"
                     /gene_synonym="BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2;
                     KATP2; KCNJ7; KIR3.2; KPLBS"
                     /note="major polyA site"
ORIGIN      
        1 gcttttatct cggagccgcg tcgcttcgtg cgctgccagc ggggctcagc cagtcggctg
       61 gaattgagta gcattcattg aatctgcgga tttcatgacg tctctctgcg tggtccacca
      121 cttttctcct aaccggggat tttttttttc ttctgccact cttatctttc cccacttcat
      181 tccacccagt ctccctcccc cgtccctgcc caaacgcgcg cccctccgcc cctcccttgg
      241 ccccagcgcc cagccctgct ctccgcgctc ggccagaggg agccagtccg gagacggccg
      301 cacctggctg gagaggctgg gcgggcggag gggtggagac ccgcggacgc cgggaagccg
      361 gacctggagc cggagcagcc gcgagcagaa tggagtctcc taacagcctc tcggtgctga
      421 tgtgaaattt gaccatctga ttccagtttt tttcttttcc ttttcttttt tgcatttcct
      481 tccctcgcca tccgtcgtgt agtgaattgt tcagtcttgc tccgtttcaa gagaggagat
      541 catgattgag tgaagccacc ccgtccgcag ccaggaaaag cacaaagaag aaactgcaac
      601 aatggccaag ctgacagaat ccatgactaa cgtcctggag ggcgactcca tggatcagga
      661 cgtcgaaagc ccagtggcca ttcaccagcc aaagttgcct aagcaggcca gggatgacct
      721 gccaagacac atcagccgag atcggaccaa aaggaaaatc cagaggtacg tgaggaaaga
      781 cggaaagtgc aatgttcatc acggcaacgt gagggagacc tatcgctacc tgaccgatat
      841 cttcaccaca ttagtggacc tgaagtggag attcaaccta ttgatttttg tcatggttta
      901 cacagtgacc tggctctttt ttggaatgat ctggtggttg atcgcataca tacggggaga
      961 catggaccac atagaggacc cctcctggac tccttgtgtt accaacctca acgggttcgt
     1021 ctctgctttt ttattctcaa tagagacaga aaccaccatt ggttatggct accgggtcat
     1081 cacagataaa tgcccagagg gaattattct tctcttaatc caatctgtgt tggggtccat
     1141 tgtcaatgca ttcatggtgg gatgcatgtt tgtaaaaatc tctcaaccca agaagagggc
     1201 agagaccctg gtcttttcca cccatgcagt gatctccatg cgggatggga aactgtgcct
     1261 gatgttccgg gtaggggacc ttaggaattc ccacattgtg gaggcttcca tcagagccaa
     1321 gttgatcaaa tccaaacaga cctcggaggg ggagttcatc ccgttgaacc agacggatat
     1381 caacgtaggg tattacacgg gggatgaccg tctgtttctg gtgtcaccgc tgatcattag
     1441 ccatgaaatt aaccaacaga gtcctttctg ggagatctcc aaagcccagc tgcccaaaga
     1501 ggaactggaa attgtggtca tcctagaagg aatggtggaa gccacaggga tgacatgcca
     1561 agctcgaagc tcctacatca ccagtgagat cctgtggggt taccggttca cacctgtcct
     1621 gaccctggag gacgggttct acgaagttga ctacaacagc ttccatgaga cctatgagac
     1681 cagcacccca tcccttagtg ccaaagagct ggccgagtta gccagcaggg cagagctgcc
     1741 cctgagttgg tctgtatcca gcaaactcaa ccaacatgca gaactggaga ctgaagagga
     1801 agaaaagaac ctcgaagagc aaacagaaag aaatggtgat gtggcaaacc tggagaatga
     1861 atccaaagtt tagtgcccta gctgggcaaa cccttctctt ctccccccaa cacaatcttt
     1921 ccttgtctct cattctcttt ctttttctgt ctctcttgct ttgttcttta tttgtttata
     1981 tttaattttt acatgaccag aaaacaaatc ttcaaggtgt aaaatatcta cctgccctct
     2041 ctcagttatt cagattgaca aggtagacat ggatttgatg aaagtgcaaa gtgccctcat
     2101 ttgtggccca agcctggtct cctcccaaaa tactacacat ccaactcctg gagatttcag
     2161 ttacttacct gcatgtgttg tacaatacca gatcactcaa aaaggtgtgt caaagatttt
     2221 acctgggata tgacaagcaa ggtttctggt gcctatttat tcattcagtg agacacagag
     2281 tggagccctc agttttatgg atcccaattc atttcatcta ctacagggtg aggtgcttgc
     2341 ccccatgtgg gtgtggcagt tacagggccc aggtgagctg aagacaaacc actgtacata
     2401 tatatgcctt atgtaattat tttctttttg taattagtaa taaaacccag catgtacaaa
     2461 agtaccatag aacagaactg ctaaatactg tacatagatg tatcattaat gtaggtttag
     2521 atatataact ttagaaataa gaagcaaaaa aaaaaagaaa gaaagaaaga ttcctaacat
     2581 gcagtaggca tttctgtttt gatgtctgtg ttttccaggt tattttgatc cagttgacgg
     2641 ctggcccttc agtgcatggc ctttgtttgt gtctcagact ggcagacgtt agggtacctc
     2701 actgttccca cacggatcct ttcattgaat ccaacaagcc ataggtctgg gggaaattga
     2761 gaggtctggt agatggcaaa acccaccacc aatatgctga tatactgtga aatgttacaa
     2821 aacagatgtc tttttttaaa agataacaag ggcctcacta actggcctgg aggaggggga
     2881 ataccaggaa ggaaatattc tctagcctgt caccaaaggt atgtttttac ttttcacatg
     2941 agctcttgcc ctttgtagta caataattta ggaaactttt ctgcaaaacc attgattctc
     3001 cagctgtagt ttctctacac tcttacaatg agacatggta agttagcaga agatggtttg
     3061 ggtctaaagc cgtgaaattc acaaaggcat gtagtgagtg gcatgcatta taatcatcga
     3121 cctctcactt tgactctatc tggctttctg agaatgacat ctcgcctgtc cctggggctt
     3181 atccatcagc atttcttgca ttgtgatctt ttattatttt gaaatgacca gagttcagta
     3241 ttcaaagatt agatcaacaa tcttgatgat tgtctaagaa atgtctctag agtatgccac
     3301 aggctagaaa tcaaatccag tgtctgtcaa tcaaaagaaa acaaataaaa aaattccagc
     3361 cttaattcac tttaatctgc aaaaaggaaa gaagggagga agggagggag gaaggaagga
     3421 aggaaggaag ggtatttgta ctggaaatct gaattttaaa atgaaaaaaa taaggaaaca
     3481 aatcttagat caaacctccg gggcaggtgg agattttgca ctgttacaga tctttcacat
     3541 tcactttgca tttataagct gtttgttctt taacacagtt gcccatcata aatattcatt
     3601 gaatggatcc aagtttattt tcagctccta cagctgtgct cagtttgagg ctctgcacca
     3661 tggaggtcaa agagatcaaa ctagctgtcc ctctttgttt cccttgcctt ccctgacctg
     3721 atcgcacagg cctttggtgt agtctgggga ccttgtctac taaaaaagaa gtcccaggat
     3781 aaggaaagtg gctcctaaat ataacctcgg ggcctctctt ctcccatctc agagaccttg
     3841 gatcaggaca actgcctctt ggcactgact ttaaggaaaa caaaactgtt ggctatagcc
     3901 ctttcctgtt tgccagcatt tccttttttg cattgaatct ctgttgaatc atttctttat
     3961 ttcgcacact ctaagaagga ggtggtggtc cgggaggagg aagctcgata cactcccctt
     4021 gaggctgcgg ggaggagcca tggcttgacc tctctgcagc ctaatgaagg gcactcttgg
     4081 cttggggagc tcccctgcaa ccttgccagg gtcgcagggc aggccatggc aggcacaaaa
     4141 caagatggaa taggggcggc aaaatgtctt ctaatttagc tcttgaagac tcactgggcc
     4201 tttttaattc cattgctaag gtttaaagga cagcgcacac ccccgtgtct ggttggaaga
     4261 gcaagcagct cagggcctca tccctgcttg cctgcccttt agcacaactg aacgaagagc
     4321 cttgacaaca cgttggccac gtgcacctgt cagttctgtg tctgtgacgg gcacccagat
     4381 gtgaggtctc tccctgtttc tcactgtctt tgttgtcaat gtagttggag acagaacttg
     4441 aaccaaatct cgtttttgta atgtgagcct ctgaatcttt acatggggat gctcctgaag
     4501 aaccttttac tagaatgtcc ctatcaaaca tgtctatttt tctacagttg ttaacacagt
     4561 ttaacagtgt attttgtgtc aactgttaaa atttggaact gaaagttcat tgagactgat
     4621 tttctaagca gcgagtagtg agtgaatggt gttttccatt aagacagctc aacaaaattg
     4681 accccacaat ttccactcat tctattccgc aatatccagt cctcattccc tcagtcactc
     4741 cattagcaaa ccaagtgcaa tgagaatttt ttaaaagtct cttttaatac aacagtatcc
     4801 ataactaatg aagatcactc tcaactagat cctttggtct gtttggttaa tgtatttgag
     4861 tcatttttcc ataaagcatg gtgggatccg tgagccagat aagaatttgg aatttaaggg
     4921 ttatggtttg ccacagttga ccccaatttg acagaacaaa gtgctcctgg atggaagggc
     4981 aggttcagct gcaagcatga ccccccacca caaggctgct gagttcctcg gattctcacc
     5041 tccacccacc ccaacgtgct ctccaactgg aagagcaccc attcatttat aaatcaactt
     5101 tatggaacca ataaaatcat ttctgtttta gctgtcccct gggtaagcta tactctttta
     5161 cattaacata gcaaatgtca acttttctgt tccttcaaac atggccttcc agcctggttg
     5221 ttccagcata tatcccctct atgtccatga ggcagaactc tctcttcccc cactccgttc
     5281 ttccatctat ccccatcctt tgctgtttcc ctggttctcc agttccaatt ctattgtttt
     5341 agttcagtac cttatgttcc ctaacagaaa tccagctgca aatgtcaacc tatttcttaa
     5401 gcacaatttt ctcttgcagg tctaaccaag ctagggctct gaaatgtcta tttttcactg
     5461 cactccaatt taaaaattat tcatgacttc atttaatagt ctaaattttg aattctccag
     5521 agcaacattc ttatgctttc catccctaca ttttcttaca tttcagtttt cttacttttt
     5581 tcccctttgc ttttttattt agatttaagc cagtgtcttt gggatattta gaaactgctt
     5641 acaaaggtgg gaactggtgg acagagaaag ggggcataag aatgctatta gaaaactacc
     5701 tgaactcacc agaagaatgg gaaatcattt gatttgctga gagaatacaa ttaaaatact
     5761 catagtaaat catttccaaa acagtttgct aatagtctgg ctactcttca gtctttcttt
     5821 ccttgacttg ggtgatactc tgtatgattt aaaaatgtca ggaaggtagt tgaaagttca
     5881 gtgtcctcta tatattgtaa acattatctt caaggatgat ttcctttcaa tggatggctt
     5941 taacattgaa aaacataggt aacatataca taatagatga ttcattgata ttactttaca
     6001 atacactaaa ataacattat cttgtttctg aactttatgg ccaaccggta tctaagaaaa
     6061 tgttagcctt taatttaaaa ttgagtaaag cacgcctctt cctaatattt ctgcctttaa
     6121 tgtcttgagt gctttagatg cagagtgtca tctagaagtc aaaattctgt cagaaggctg
     6181 ctaagagatt ttgataaatc acagaatata taataataac ccaacaatat tttacccaac
     6241 atggtgtggt gtttgagagc attaagagca cttgtacaaa ttatcttatt tcattctaat
     6301 tcaccatgag aggttgagta ttataattta cattttatag agggaaaact agggtttggg
     6361 caaattggcc aagtttaaag gccagtgaat catagctcct tgtctcaaat ccaggtcaca
     6421 gggccctaag ctgtgggcta tttttactac aaaaaatttc agcaaaccaa atctgctaaa
     6481 aaaaaaaaaa tctggcctgc tgcctgttat tgtaccactt gtgagctaag agcaattttt
     6541 acatttttta ataattgggg gaaaaaaaag aaaagaagaa tattttgaga catgtgaaaa
     6601 tcatcctaaa ttcaaatttc aatgtccaca aacaaagttt tattggaaca cagccacagc
     6661 catttgtctg tgtattgcct atggctgctt ccacatgcac tcacagagct gagtaattac
     6721 aacagagacc gtgtggccag caaagattga aatatttact atctggccct gtataaaaaa
     6781 agtttgctga cccctgtact gtactgttaa gtactattgg tactattaag tactagtaat
     6841 agtactatta ataatagtat gtaagagtac taagtactat gaaatattgt actgtactct
     6901 taagagagtc ctaggcaata tagaagaatg actttttttc tagcataaat tattgaagtc
     6961 actcataagt ctgttcaaaa taacataact cttttttttc ggttgtcaaa gagacaactg
     7021 cttcacaaga cttagagagc ccagtcattc agttagggtc tcaaaggaag aatccttcct
     7081 tttcatgcca ttcctccaga gtggcaaata gagtcaacag ccagccagga tctagcggtc
     7141 aactcagaaa gtggatacag taaaattact ccttaatgaa ctacagagaa atgggcattc
     7201 ccagcccagc cgctgaaata ttgcttcagt ggcaccctca ggcgttggat gatgtacata
     7261 gctcaggctg ccagtctctg ctcagcaaag cagcccagac ctgcagtggt tgccacacat
     7321 caaacccgcc tcgctcctcc acatggcttc tctcttaggc tctcttggag gagagttaaa
     7381 taggtggccc agatgtctcc ctctgttttc ttgctagttt tctctagcct acagtttaca
     7441 actgatcaag gtgatgaacg tgaacctgga actagctcat gtttaattgc gctagttttt
     7501 gctctctcta gccactaagc tagaagttta atgtgtaatt aaagaactaa atgcttctta
     7561 tggaaaacca aaaggtcctt tgaaaagagc tcagggagac acggaagtat agtaaagggt
     7621 tgttggattt cttgcgcttt ctgagtctga agcaaagaca aagattggta attacagaca
     7681 taatggatgg aaaaagtcca gaccttgggg tttttagatg agtcaatatt aaatggcatt
     7741 ctttgtgtca ctaagctgaa ctcccttgtc ttctaaggaa gcatttctaa tgccagaggt
     7801 agtcagccaa gctaggttcc ttatcttttt agttctatgg aaaaacatat caagttctct
     7861 tatgagtgct ttcctggaaa tcagatgaga aagaaaaaaa agtaacaatg taacaaatca
     7921 ttgtgatgtt gttatattgt cgacagataa aggatggatt tgatcctgcg tctgaaacta
     7981 agtaggtaat ggtatctaca atatagatca tgcaaaaagc ttgtgaccct tcaactgcat
     8041 ggaagagtgg gaggaaacaa ctaagtggga ggaaacaaat ctcaaagttc aacaccataa
     8101 aacttaaata aatgtaaaag gagtcaaact tttacttggc ctcattgcct tggtaaaaga
     8161 aagcttcatg tatccaccta attcaataag agagtcctag gaaaaggaag gcaaaatact
     8221 ttaaaggcac acaaaaatat ttcactgggg tggtcaacat gattaagtga tggagattag
     8281 atttatcact gatgaaaaca gtatttatag agttattttt agacactata ccccctagat
     8341 gcaaatccca acatgcactg tggttggccg gagaattgtg gccacatagt aaacagatct
     8401 tgatttcaga gaatgaaatg ttcctaccga ctttgtgtaa ttgcccattt caagattaag
     8461 gctcagtgcc cattgtttta acaccgatca tcaatactgt gatatcaact aagccaatct
     8521 ttataagaat atgaagggct gtttttctca ggcaatgtaa atatctgcta ccaacaagca
     8581 ctagtgaaat aaacatgtaa tcatcatcgt ttctaaaact ctgatagaaa tcacaaaaca
     8641 ttcaagcaaa agggcaaggt cttccaggag cctaggagca gacttttggc tacaaagagc
     8701 ctagtccagg ctgaatgcct tatggagtat gtcagtcttg ggtcttatca ctaaacacaa
     8761 ctagtagaac tcgatcaact ggagtgcaga gatcagagtt tcggtcagcc gtgcattacc
     8821 caaacctctg ggtcttcagc cagtcttctt agaagagggg aaggagcact gtcctcagat
     8881 ccggccatca cgggcatcgc cctgacatca tctctcagca ttgctgactg tgggcatggg
     8941 tatgagaaat accctcccgt agctcagact tgggcagaac aaaatcctca gccatgccca
     9001 cccactgagg tctctctagg ggagcttctc cctgcagggt ctggctcttg cccatctgga
     9061 caatgacccc ctagaagtat cttccggggg accctgtatc tttcaagctt aacttgcccc
     9121 acacccttga ccccacagag cacctccctg ggtcaggaat gatgactaaa ttaagacctt
     9181 tagcatctgc taagcacagg ggtgccctcc catgtgaaag tccaagtaaa ataaatggag
     9241 tcagggtaag gaaatcccca aaagttctga ttttctcatg gtgatgatct cagtgctttt
     9301 ctttttcatg acatatctaa ttctcttttc tctaggaagt ctcacgtttt ggtatcacga
     9361 tgccgtgggc acagactcag tcatcgggcc aatggcagaa ctctctccag atagtctgac
     9421 ctggtccctc tttttgagta aggctgggag tttggtttgt ttgagccacc ccacaaccaa
     9481 tagaatccca ccctggctaa gaaatctcac aaggccgggt gctgtggctc acacctgtaa
     9541 tctcagcact ttgggaggcc aaggtggcag atcacctgag gccaggagtt tgagaccagc
     9601 ctggccaata tggtaatacc ccgcctctac taaaaatatg aaaaagttag ctgggcatgg
     9661 tagttcctgc ctgtacaccc agctactctg gaggctgagg cacaagaata gcttaaactt
     9721 gggaggggga ggttgcagta agctgagatc atgccactgc actccagcct gggccacgga
     9781 gcgagactca gtctcaaaaa aagaaagaaa agaaggaagg aaggaaggaa ggaaggaagg
     9841 aaggaaggaa ggaaggaagg aaaaaagata aagaaagaag gaaggaagga aggaaagaaa
     9901 gaaggaagga aggaagaagg aaagaaagaa agaaaagaaa gaaagaaaaa gaaagaaaag
     9961 aaaagaaaag aaaagaaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag
    10021 agaaaagaaa gagaaagaaa gaaagagaaa agaaatcgca catccccacc tctatcaggg
    10081 tcaccaaatg aaggcatcaa tttcaagtcc cgttattatc tgcaaggaac tctgcattgc
    10141 agcacttaca gacacaactt atgctgtctc ctggtgccca cacagctttc gggtgatggc
    10201 agattgtcag ccaaccattc cccccagctg ctctctctta atttgctccc tcatctcacc
    10261 acgaaaactt cacttttcct ggcacccccg ttggaaggtt aatatcattt ttgagcatcc
    10321 acaatccagc aacctattat tggggttagt cctattcctt accacttaat agccaccacc
    10381 aattttaacc gagcgtatat gttcagagtc taaccatata tatatatata tatatatata
    10441 tgtacatttt gttcctgctt cataattgcc cagagagtct atgagtgatg gttgggcgta
    10501 tacatggtct cagcccggta actgacgaga gcagcataaa gttaggtctt gtgctgaaag
    10561 gacagctgga ctggtccagt gggatcttct tgtcctttgc agcatgccat taggggacca
    10621 gcttgccaaa gaagctcctg aacatcttta ttcagcccag tgcttcctcg tcttaaccaa
    10681 gagccacaca gagacaacag tgaactcaca agttcatagg attccaaaat gattgactcc
    10741 ccaagtgtct gcctgtgttg gcaaagtgct ctttcctttc ccactgtctg cacagactgt
    10801 actctgggga aactcccagc acttgtgggt gcctggttac cgaggatgtg cttggctgcc
    10861 atggtaactc ccagcctctc agtgtggacc actctcccgc aggctcgagg agacctggca
    10921 gaggccccat ggggcggagg ctccctaggg ctgctccacc tgcagacagc cttgggagaa
    10981 cccaaggtaa tgtggagtag gacccagcac acaggagctc cctccgcacg ccaggtctgg
    11041 ggtattccca tcctttcttc ctaagatggg atgcacttag gtggccaaat tgcttcttaa
    11101 aaggctcttc ttcaagccat catcctcata tcacactcat gcccacactc cagtcacatt
    11161 tgcctttaaa aaattttcaa aattattttt gtattatccc atgtaacaga tgtccaaaga
    11221 gttgccattt tcttaacact aaataaattc tgcctagaag attaagattg gtccagaaca
    11281 gggagtgttt taataagcac aaatataaac aaaagcaatt ttaaaattta cttatggatt
    11341 ctttcagaaa acctgatttc aaatcagaaa tactcatttt tgcccccaga gcaaagttcc
    11401 agaatactat ttcttcttga acttgccatt ttccatctgg ctttgaactt gagaatagac
    11461 tcccctatat tggcataaaa ggtaaagtgg aatttaccac tgaaactttc agagctcaat
    11521 ttagtctggc cccatggacc aggcaagaat aaactctgct caccagtaac aattctgcag
    11581 ccaccgtact gaacacaaac cctggagaaa ttctggacat ggtactgctt aagaatgctg
    11641 ggtactcggg aggctgaggc aggagaatgg cgtgaacccg ggaagcggag cttgcagtga
    11701 gccgagattg cgccactgca gtccgcagtc cgacctgggc gacagagcga gactccgtct
    11761 caaaaaaaaa aaaaaaaaaa aaaaaaagaa tgctgggaat gtcagtcgag ggtctgattt
    11821 cagaaagagg acattaatat tcaaatccct gaaccatgcc cacactgtgg gagtcaggcc
    11881 taacaagatc ccagggtcca tagtttacct ggtcagcttg ctgtgtctca tggggcatta
    11941 gagggtagat gagaactttc cgcaatctcg acaactgcac tgtaagaatt tcttctgtgt
    12001 attttctaat tctgtgacaa caggcatcaa caaaacatgt ggcctgttat cacatggttc
    12061 ctccctgtgt gcaccttcat agagattttt tccttttcta aaagaatgag gattcctctg
    12121 aatgttacac tatgcaacaa taatgtcccc agtccttcat aagtaacaca gtaacaatat
    12181 aagcaccctc tccctacata acacacacac atacacacaa atacacacac agacacatgc
    12241 atacacacac acatacacac agacacatgc atacacacac atacagagac atgcgcatac
    12301 acacacacgc atacatgcac atgcatacac acaccaagac atgcatacac acacgcatac
    12361 acagagacac atgcatacac acacgtgcat acacacacag acatatgcat acacacacgc
    12421 atacagacat gcatacacac acatgcatac agagacacat gcatacacac acgcatacac
    12481 acacacgcat acacagagac atgcatacac acatgcatac agagacacat gcatacacgc
    12541 acacagacac atgcatacac acacacgcag acacagacac atgcatacac acagagatac
    12601 acgcatactc acacagacac agacacatgc atacgcacac acagatatac acacacacac
    12661 acacacacac actcttatcc tttccaatca gttatgagtg acaccacaaa agtaaatttc
    12721 cagaaatcaa agccttaaga ttggcgttcc tcccctgcat gcactgtggc attattttaa
    12781 aaatgtacca tacacttccc tgaacctgca ggcaacagcc tttcttccag tgtttggtag
    12841 gaaccctgga ttcagaagac caggttttgc ccccagatga gcaatgtcgt cctgggtata
    12901 tcatttcact ggtcggaaat agcttcctcc accccttttt taaaaacttg ataaatttta
    12961 ttttttagag cagcattaac ttcacagcaa aatagagcag aaattaccaa gagctctcat
    13021 ttacccccgg cccccgcatg catggccgcc cccacgagtg atatgccaaa ccacaatggt
    13081 acatctgtgg aaatggatga gtctacactg agacattact atcaccccag agtccattgt
    13141 ttacaatggt gttgtacatt ctatggattt gggcaaatgt ataaaggcat ggatccacca
    13201 ttatagaatc atacaggata gtcccacggc cctaaaaatc ctctgtgctc tcccaattca
    13261 tcttgtgtcc tcccaacccc tggcaaccac tgatctattt actgtctcca tagttttgcc
    13321 ttttccagaa tggcatataa ttggactcat gcagtacgca gccttataaa attggcttat
    13381 ttcacttagt aacatgcatt taagtttccc tcatatcttt ttatggcttg agagctcact
    13441 tttttagcac caaacactca tccattttct ggatgtacca ctgtttattt atccattcac
    13501 ctactgaagg acaccttggt tgcttccaag tttggtcagt tatgaataaa gccactataa
    13561 atatctgtat gcaggttttt gtgtggacat aagttttcaa ttcatttggg taaatatgat
    13621 atgttaaagc tatatttagt cttgcaagac acagccaaac tgccttccaa agtggctgta
    13681 ctgttttgca ttccactagc gatgaatgag ggttcccgtt gctccatatc ttcaccagca
    13741 tttagtgttg tcaatgatct gcatttgggc cattctaaaa gatgtacggt ggtgtttcat
    13801 tgctgtttta atttgcaatt ccctaatgac atgatttggg acatattttt atatgcttgt
    13861 tttccatctg tacatcttct ttggtgaggt gtctgttcag gtcttttggg cattttttta
    13921 aatcgggttg ttttcttatt gttgatttgt taaagttctt tgtaaatttt agataataat
    13981 tctttatcag atgtgtcttc tgtaaatatt ttctcccagt ccgtggcttg tcttctcatt
    14041 atcttgacct tgtctttttc agagcagacg cttttaattt tactgaagtt cagcttatca
    14101 tttacttctt tcatgatcat gtctttggtg ttctatctaa aaatctattg ccatatccaa
    14161 ggtcatctaa gttttctctt atatgttacc ttctaggagt tgtatagttt tgcattttac
    14221 attagatctg tgaccgattt tgagttaatt tttgtgaaaa gtgtttgtta ggtctgtatt
    14281 tagactcttt tttttttttt tttttttgcc tgtggatgtg caaatgtgca gctatttcaa
    14341 caccgtttgt tgaaaagacc ctctttgccg cactgttttg cctgtgctcc tttgccaagg
    14401 atcagttaac tatatttgtg tgggtctgtt tctcagctct ccattctgtt ccactgatct
    14461 atttgtctat tccttcacca acaccacatc gtcttgagta ctgtagcttt ctttctccat
    14521 ataaactgca gactcagttt tttggtacca acaaaataac ttgctgagat ttttattggg
    14581 attgtattta atctacagag gaagctggga agtgacatct tgacaatatt gagtccctcc
    14641 atgaaatgga atatctctgt atttacttag ttcttctttg atatcattca ccagttttgt
    14701 agttttcctc atgtagatct tatacatatt gttagattta taccttaagt acttcatttc
    14761 aagggatact aatttaaata atcatgtgct tttaatttca aatttcactt attcattgcc
    14821 ggtatatagg aaaacaattg actttgtata ttaactttga atcctaaaat cttgctataa
    14881 tcccttatta gttccaggaa ggttctgggg cttgtttttg ttttgttttt tggtcagtta
    14941 tttcagattt tctacataga tgatcatgtc atctgtaaac aaagacaaag ttttatttct
    15001 tctttcttcc caatctatat atttttattt cctttccttg tcttattgca tcagccagga
    15061 cttccagtat catgttgaaa agcagtagtg atggggacac cttttttttc ctgatcttag
    15121 tgagacagct tcaggtttct caccatcacg ccatttagta tgatgttagc tgtaaggttt
    15181 tttttttaga tattgtttat caggttgagg ggcttctcct ctattcctgc tttactgagt
    15241 gtttttatca tgaaagactg tttggatttt tctgcatctg ttgatatgat catgcaattt
    15301 ttcttctttt gcctgttgat attcattaca ttagttgatc tttgaatgtt gaaccagcct
    15361 tatataccta ggacaaattc cacctggtca tggtgtttaa ttattttcat atattgttgg
    15421 acttgatttg ctaatatttt gttgaggatt tctgcatcta tgcttatgac aggtattggt
    15481 ctgtagtttt cttgtaatgt ctttgtctgg ttttggtatt aaggtgatgc tggattcata
    15541 gaatgagtta gcaagtgttc tctctgcttc tagcttttga aagagattgt agataatcag
    15601 tatcatttct tccttaagtc tttggtagaa ttcaccagtg aacccatctg ggccaagtgc
    15661 tttctggttg gggaggttac taaccattga ttcaaattat ttaacagaga tatacctatt
    15721 cagattgtct atttgctctt gtgtgagttt tgacaaattc tatttttcaa ggaattggtt
    15781 catttcatct acattatcaa atttgtgggt ataaagtcat tcataaaatt ccttcattat
    15841 cttttattat ccttttaacc tccatgggat tgctagtaac gtccccgctc tcatttctga
    15901 tagtagtaat ttgtgttctt tctctttttt tcttagttag catgccttga gacttatgga
    15961 gcttattgat cttttcaaag aatcaacttt gtttctattt attttattga tttcctattt
    16021 tcaatttcgt gaatttttaa tctaatttta attatttctt ctcattggct tactttgggt
    16081 ttaatttgct ctttttttct acttttttaa aatggaaact taaatgattg atttcagatc
    16141 ttcttttgtg ctatctgcat tcaatgttat aaatttccct ttaagcattg tttttattgc
    16201 atcccacaaa ttataataag ctatgttttt attttcattt atttcaaaat attttttcac
    16261 ttatcttgag atttcttctt tgacccatga tatttacacg tgtgttgttt aatctccaag
    16321 tattttggca ttttccagct acctttctgt tactgatttc tagcttaatt ccattgttat
    16381 ctaagagcaa acattgtagg acttgtattc cttttttttt tttttttttt tttaagacag
    16441 agtctcactc tgttgcccag gctggagtag agtggcatga tcttggctca ctgcaacctc
    16501 tgcctccggg gttcaagcga ttctcctgcc tcagcctccc aagtagttgg gactacaggt
    16561 gcccaccacc aaacctggct aatttttgta tttttaatag agatggggtt tcagcatgtt
    16621 ggccaggctg gtcttgaact cctgatccca agtgatctga gaatttctat tcttttaaat
    16681 gtgttaaggt atgttttatg acccagaatg cagtctaact tgatgaacat tccatgcaag
    16741 cttaagcaaa aagactaatc tgctgtagtt tcctcatctt tttcaacaaa catttgaacc
    16801 ccatagtctc tgctttaata attctagtat ccattccaac ctacaccacc atcagttcta
    16861 gactgtacct ctacactaaa tgaccccaga tggttcagct ttaagtctca gctgttttca
    16921 tcatttaact gattcccttg ctggcagcag ggaaattaac taccagccac acacttgtct
    16981 ccaacagcac ccaccgacca cgtcctgctc ccctgtcatt accaccacta aacccggtag
    17041 cagagagatg agctgtgagg taggtgcctt ctagataaat gaggtgctac tggacatgca
    17101 ctgtggtctt ctctgcagta gaaaaatgtc caaggaaggt cagcatcatt gatagaaaat
    17161 aaatgtgagg ctgcatctcg ccagctcatc catgagcaat tccttcagct ccagcaggct
    17221 actgacaatt tgcttttaac ccaaatccag ctctaccctc cctaaagcat gcacctaggg
    17281 ccccagtcag aaaaacaaaa caaaaactaa accagacagg ataattaagg aggcaaaaag
    17341 cttaaaccat aatattttaa ttaagctgat ttattttaac cgtagcacta ctgctttatt
    17401 ttagttatat accagtaatg gaaaatagtg gccacttttc attattaatc ataagataat
    17461 tcattgtatt tgtctttcct aataaaatgg cattatatta aggcccaaaa tgtttactat
    17521 ggcccagtaa aatatagggt tgttctgtga gctatttctt aggatagaaa attattccag
    17581 aaagctatta gttcaaaagt ctctagcagt gccaaggttt caatagtctc tttaattttg
    17641 aatctatgcg attgaagata agactgcatc tagtttgggg gttgcatgtt tgtggacatt
    17701 catgtttcta tgtacctgga acttcttatt ctcaccatca cacagaattt tcaagaacta
    17761 gttaagcaaa gaaagcaaag cccagcaggt gtcagaattg ggtccccaag ctgagggtca
    17821 ttagggtgca tgtcagtgtg ccaactccaa tatccacacc tagagaacgc acatcttccc
    17881 aaaagaaatg aagcacagat gatacttgat cctcaacttt gtctccagaa attttttcag
    17941 gaacttgagt tctttatcaa atgctcacat cctacagaga aataaagtgt tccattttca
    18001 aggttcatga agctcgtcaa tgctgggagc aggcacacac ttgtattttt atactcaggg
    18061 atcccaacag gcaactaaaa ctagacaagg acaaaactcc actcaaaata ttctgaaagt
    18121 tagctgatgc cccatatttt attactccaa atgtgtattc acttgcctga gtgctggcca
    18181 tgagcactca gtgaacactt gcagtacagt gagtgatgtc cttgacaagt tactatgaag
    18241 actctgagaa aaagtaacat cccccactgt cctcaacaag cctgtaatcc catggctgcc
    18301 agcccttacc atgcaacaac actgcgctaa gtgcatcgaa ccacacaaaa ccctgtgaga
    18361 aaatactgct tcccacttca cacgtgaagt aactgagtct agttttagaa atacctaaac
    18421 gaagagggac aggcacaaca aatttgctgg aatgtaaaga aagtagatca gtttctgcgt
    18481 aagcagtaag aaatggcctc atgaggaaga aagggacatt tgccctgaca tttctcaaag
    18541 actgcttagg ttttggagac agagatccaa gggcaggcaa gcacaatggt tcatgcctgt
    18601 aattccagca ctttgagagg ccaaggtggg aggattgctt gagcccagga attcaagatt
    18661 agcctgtgca acacagcaag accccatctc tattttaaaa agagagagag agatccaaaa
    18721 gcaaaggcat ttcagtttgc aaggccagca tgggagaggg tcttaaaaca gaaacacaca
    18781 aggtaagatt gggggatggt acctagacca gcctaggatc ataggatttg ataaaatatg
    18841 catttggaaa actggtgcta atttatagag acttagaatt tcaggctaag gacaaggact
    18901 aagaaacgaa tagcatgaac agtattattg ataaacccat cattgcatct taaaggctct
    18961 gaagtcttta gtattgaatt aaaaaataat tttcactgga caaaaccaaa aggtctttac
    19021 acaactgtca agcactttga aatgattcag caaaaagcag aataaatcac tagtggaatt
    19081 tcaatagcat gtagttgtaa accatcatgt gtccagcaaa atcacaaaac aagagtttat
    19141 tttagaaggg acttagggat attaagaaat ataacataca aaataactaa agttttctcc
    19201 aagcaaaacc aatgatcctt tagggatgaa cagaaagaaa acaagaaatg cttctttcaa
    19261 aagttgctac caaggcttct aagatttctt tggaataaag tcactaagat ttgcttctga
    19321 ggtcaatctt gccaactttt ccaccatgct ttttaggaag gaacaccagg tagggctcaa
    19381 gtactggttt ggctcctgtc ccattttttg gtccttttgt aactcccttt actcaactcc
    19441 aagtatgtct gtgcacggac aatgagacga aagcatgctc aatgcgaaag cacaaattat
    19501 gtattgagaa atcactacaa tgccaaattt ttttaaaata aaaaaaaaat atatatatat
    19561 atatatatct ttaagaactc gctgtgttag ggaaactgtt tacatattga ggtgttcctc
    19621 tttttttgac tggaacaata aaagaaaaat ttgatatgg
//
