LOCUS       NM_002239               4628 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens potassium inwardly rectifying channel subfamily J
            member 3 (KCNJ3), transcript variant 1, mRNA.
ACCESSION   NM_002239
VERSION     NM_002239.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Black,K.A., He,S., Jin,R., Miller,D.M., Bolla,J.R., Clarke,O.B.,
            Johnson,P., Windley,M., Burns,C.J., Hill,A.P., Laver,D.,
            Robinson,C.V., Smith,B.J. and Gulbis,J.M.
  TITLE     A constricted opening in Kir channels does not impede potassium
            conduction
  JOURNAL   Nat Commun 11 (1), 3024 (2020)
   PUBMED   32541684
  REMARK    GeneRIF: A constricted opening in Kir channels does not impede
            potassium conduction.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Schratter,G., Scheruebel,S., Langthaler,S., Ester,K., Pelzmann,B.,
            Ghaffari-Tabrizi-Wizsy,N., Rezania,S., Gorischek,A., Platzer,D.,
            Zorn-Pauly,K., Ahammer,H., Prokesch,A., Stanzer,S., Devaney,T.T.J.,
            Schmidt,K., Jahn,S.W., Prassl,R., Bauernhofer,T. and
            Schreibmayer,W.
  TITLE     GIRK1 triggers multiple cancer-related pathways in the benign
            mammary epithelial cell line MCF10A
  JOURNAL   Sci Rep 9 (1), 19277 (2019)
   PUBMED   31848385
  REMARK    GeneRIF: GIRK1 triggers multiple cancer-related pathways in the
            benign mammary epithelial cell line MCF10A.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4628)
  AUTHORS   Yamada,N., Asano,Y., Fujita,M., Yamazaki,S., Inanobe,A.,
            Matsuura,N., Kobayashi,H., Ohno,S., Ebana,Y., Tsukamoto,O.,
            Ishino,S., Takuwa,A., Kioka,H., Yamashita,T., Hashimoto,N.,
            Zankov,D.P., Shimizu,A., Asakura,M., Asanuma,H., Kato,H.,
            Nishida,Y., Miyashita,Y., Shinomiya,H., Naiki,N., Hayashi,K.,
            Makiyama,T., Ogita,H., Miura,K., Ueshima,H., Komuro,I.,
            Yamagishi,M., Horie,M., Kawakami,K., Furukawa,T., Koizumi,A.,
            Kurachi,Y., Sakata,Y., Minamino,T., Kitakaze,M. and Takashima,S.
  TITLE     Mutant KCNJ3 and KCNJ5 Potassium Channels as Novel Molecular
            Targets in Bradyarrhythmias and Atrial Fibrillation
  JOURNAL   Circulation 139 (18), 2157-2169 (2019)
   PUBMED   30764634
  REMARK    GeneRIF: Mutant KCNJ3 and KCNJ5 Potassium Channels as Novel
            Molecular Targets in Bradyarrhythmias and Atrial Fibrillation.
REFERENCE   4  (bases 1 to 4628)
  AUTHORS   Tateyama,M. and Kubo,Y.
  TITLE     Gi/o-coupled muscarinic receptors co-localize with GIRK channel for
            efficient channel activation
  JOURNAL   PLoS One 13 (9), e0204447 (2018)
   PUBMED   30240440
  REMARK    GeneRIF: Gi/o-coupled muscarinic receptors co-localize with GIRK
            channel for efficient channel activation
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4628)
  AUTHORS   Kammerer,S., Sokolowski,A., Hackl,H., Platzer,D., Jahn,S.W.,
            El-Heliebi,A., Schwarzenbacher,D., Stiegelbauer,V., Pichler,M.,
            Rezania,S., Fiegl,H., Peintinger,F., Regitnig,P., Hoefler,G.,
            Schreibmayer,W. and Bauernhofer,T.
  TITLE     KCNJ3 is a new independent prognostic marker for estrogen receptor
            positive breast cancer patients
  JOURNAL   Oncotarget 7 (51), 84705-84717 (2016)
   PUBMED   27835900
  REMARK    GeneRIF: these data suggest that patients with estrogen receptor
            positive breast cancer might be stratified into high risk and low
            risk groups based on the KCNJ3 levels in the tumor
REFERENCE   6  (bases 1 to 4628)
  AUTHORS   Signorini,S., Liao,Y.J., Duncan,S.A., Jan,L.Y. and Stoffel,M.
  TITLE     Normal cerebellar development but susceptibility to seizures in
            mice lacking G protein-coupled, inwardly rectifying K+ channel
            GIRK2
  JOURNAL   Proc Natl Acad Sci U S A 94 (3), 923-927 (1997)
   PUBMED   9023358
REFERENCE   7  (bases 1 to 4628)
  AUTHORS   Liao,Y.J., Jan,Y.N. and Jan,L.Y.
  TITLE     Heteromultimerization of G-protein-gated inwardly rectifying K+
            channel proteins GIRK1 and GIRK2 and their altered expression in
            weaver brain
  JOURNAL   J Neurosci 16 (22), 7137-7150 (1996)
   PUBMED   8929423
REFERENCE   8  (bases 1 to 4628)
  AUTHORS   Schoots,O., Yue,K.T., MacDonald,J.F., Hampson,D.R., Nobrega,J.N.,
            Dixon,L.M. and Van Tol,H.H.
  TITLE     Cloning of a G protein-activated inwardly rectifying potassium
            channel from human cerebellum
  JOURNAL   Brain Res Mol Brain Res 39 (1-2), 23-30 (1996)
   PUBMED   8804710
REFERENCE   9  (bases 1 to 4628)
  AUTHORS   Chan,K.W., Langan,M.N., Sui,J.L., Kozak,J.A., Pabon,A., Ladias,J.A.
            and Logothetis,D.E.
  TITLE     A recombinant inwardly rectifying potassium channel coupled to
            GTP-binding proteins
  JOURNAL   J Gen Physiol 107 (3), 381-397 (1996)
   PUBMED   8868049
REFERENCE   10 (bases 1 to 4628)
  AUTHORS   Stoffel,M., Espinosa,R. 3rd, Powell,K.L., Philipson,L.H., Le
            Beau,M.M. and Bell,G.I.
  TITLE     Human G-protein-coupled inwardly rectifying potassium channel
            (GIRK1) gene (KCNJ3): localization to chromosome 2 and
            identification of a simple tandem repeat polymorphism
  JOURNAL   Genomics 21 (1), 254-256 (1994)
   PUBMED   8088798
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U50964.1 and AC093633.3.
            
            On Nov 22, 2018 this sequence version replaced NM_002239.3.
            
            Summary: Potassium channels are present in most mammalian cells,
            where they participate in a wide range of physiologic responses.
            The protein encoded by this gene is an integral membrane protein
            and inward-rectifier type potassium channel. The encoded protein,
            which has a greater tendency to allow potassium to flow into a cell
            rather than out of a cell, is controlled by G-proteins and plays an
            important role in regulating heartbeat. It associates with three
            other G-protein-activated potassium channels to form a
            heteromultimeric pore-forming complex that also couples to
            neurotransmitter receptors in the brain and whereby channel
            activation can inhibit action potential firing by hyperpolarizing
            the plasma membrane. These multimeric G-protein-gated
            inwardly-rectifying potassium (GIRK) channels may play a role in
            the pathophysiology of epilepsy, addiction, Down's syndrome,
            ataxia, and Parkinson's disease. Alternative splicing results in
            multiple transcript variants encoding distinct proteins. [provided
            by RefSeq, May 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1; also known as
            GIRK1a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.54688.1,
                                           SRR1803613.164476.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295101.3/ ENSP00000295101.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1118              U50964.1           116-1233
            1119-4628           AC093633.3         120534-124043
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.1"
     gene            1..4628
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /note="potassium inwardly rectifying channel subfamily J
                     member 3"
                     /db_xref="GeneID:3760"
                     /db_xref="HGNC:HGNC:6264"
                     /db_xref="MIM:601534"
     exon            1..783
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1587
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     inward rectifier K+ channel KIR3.1; G protein-activated
                     inward rectifier potassium channel 1; GIRK-1; inward
                     rectifier K(+) channel Kir3.1; potassium channel, inwardly
                     rectifying subfamily J member 3; potassium voltage-gated
                     channel subfamily J member 3"
                     /codon_start=1
                     /product="G protein-activated inward rectifier potassium
                     channel 1 isoform 1"
                     /protein_id="NP_002230.1"
                     /db_xref="CCDS:CCDS2200.1"
                     /db_xref="GeneID:3760"
                     /db_xref="HGNC:HGNC:6264"
                     /db_xref="MIM:601534"
                     /translation="MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQ
                     RFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMW
                     WVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGII
                     LFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNL
                     RNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDA
                     KSPFYDLSQRSMQTEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLE
                     EGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGL
                     DDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEK
                     LVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT"
     exon            784..1000
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..4628
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4606..4611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /note="hexamer: AATAAA"
     polyA_site      4628
                     /gene="KCNJ3"
                     /gene_synonym="GIRK1; KGA; KIR3.1"
                     /note="major polyA site"
ORIGIN      
        1 acctccttat tggtgctagt ttgcagcgcc cagctcctgc gccttcgctt cgcgtttgaa
       61 tctggctcgc cccttcgtat tatgtctgca ctccgaagga aatttgggga cgattatcag
      121 gtagtgacca catcgtccag cggctcgggc ttgcagcccc aggggccagg ccaggaccct
      181 cagcagcagc ttgtgcccaa gaagaagcgg cagcggttcg tggacaagaa cggccggtgc
      241 aatgtacagc acggcaacct gggcagcgag acaagccgct acctctcgga cctcttcacc
      301 acgctggtgg acctcaagtg gcgctggaac ctcttcatct tcattctcac ctacaccgtg
      361 gcctggcttt tcatggcgtc catgtggtgg gtgatcgcct acactcgggg cgacctgaac
      421 aaagcccacg tcggtaacta cacgccttgc gtggccaatg tctataactt cccttctgcc
      481 ttcctcttct tcatcgagac ggaggccacc atcggctatg gctaccgata catcacagac
      541 aagtgccccg agggcatcat cctcttcctc ttccagtcca tcctgggctc catcgtggac
      601 gccttcctca tcggctgcat gttcatcaag atgtcccagc ccaagaagcg cgccgagacc
      661 ctcatgttca gcgagcacgc ggtgatctcc atgagggacg gaaaactcac gcttatgttc
      721 cgggtgggca acctgcgcaa cagccacatg gtctccgcgc agattcgctg caagctgctc
      781 aaatctcggc agacacctga gggtgagttc cttccccttg accaacttga actggatgta
      841 ggttttagta caggggcaga tcaacttttt cttgtgtccc ccctcacaat ttgccacgtg
      901 atcgatgcca aaagcccctt ttatgaccta tcccagcgaa gcatgcaaac tgaacagttc
      961 gagattgtcg tcatcctaga aggcattgtg gaaacaactg ggatgacttg tcaagctcga
     1021 acatcatata ctgaagatga agttctttgg ggtcatcgtt tttttcctgt aatttcctta
     1081 gaagagggat tctttaaagt tgattactcc cagttccatg caacatttga agtccccacc
     1141 ccaccttaca gtgtgaaaga gcaggaggaa atgcttctca tgtcgtcccc tttaatagca
     1201 ccagccataa ctaacagcaa agaaagacat aattctgtgg aatgcttaga tggactagat
     1261 gatattacta caaaactacc atctaagctg cagaaaatta ctggaagaga agactttccc
     1321 aaaaaactct tgaggatgag ttctacaact tcagaaaaag cctacagctt gggagacttg
     1381 cccatgaaac ttcaacgaat aagttcagtt ccgggcaact cagaagaaaa actggtatct
     1441 aaaaccacca agatgttatc tgatcccatg agccagtctg tggctgattt gccaccaaag
     1501 cttcaaaaga tggctggagg agcagctagg atggaaggga accttccagc caaattaaga
     1561 aaaatgaact ctgatcgctt cacataacaa agcactccct taggcattat ttaatgtttg
     1621 atttagtaat agtccaatat ttggcgatga ggtaattctc cctaaggaat ctgaaagtat
     1681 attttcctcc cagttctaca agcatatttg agaacccttc ctttcccaag tattgcgaat
     1741 gtgcagaaag caacagttac ggagggagga catcataagg aagttattaa cgggcatgta
     1801 ttatcacatc aagcatgcaa taatgtgcaa attttgcatt tagttttatg gcatgattta
     1861 tatatggcat atttatattg tatattctgg aaaaaaaata tatatatata tttaaagggg
     1921 agatactctc cctgacattt ctaacatatg tattaagcca aacatgagtg aatagctttc
     1981 agggcgataa aactaaatat atgtctgtgt gtgtgtgtgt atgtatacac acatatacat
     2041 atatatatac acatacatac acatacatac atacatacat atatatctga taaaattgtg
     2101 atgttttgtt caaagttgta gttcttgtgc atgtttactt tattagagta ggaaggctac
     2161 tggcattaat tattaatacc aaatatttta gccttaaatt tttgtcattt taaaatctga
     2221 tttaatgttt tctgctgttt aaggtcttgg gaggctttca attgtatttt atatgagaga
     2281 atcacacaag tttgtgctat ctatggccct gcaaaaatat aaccattaca tgtttaaatt
     2341 gtaaatttta gagcatacca gtactcagta tagcattgaa catttcttat gatttttaaa
     2401 agttgctagt actggggaga aataattgtt gattaatttg agaattattc ctttcctaga
     2461 ctaattaaaa tctggaaatc tgttttgtat atgatctaat acaaagatga gctctgaaca
     2521 aacactgaat catgttaata gacagtagcc aagttatatt gaatatatca gaatctgtgt
     2581 gaagttacac aattaattgt ccctgtttca aactgagtaa attggaaaca ttttctttct
     2641 ttttctggaa attttgtcca ttttaaaaac caatcatttt aagaagacat gacaatgcaa
     2701 tgaaacagat gataaatatt tatgcttaaa atatgtatgt ctaattgagt ctctttttta
     2761 ttctgttttc ttgtttatgg catttgttgt aacaggatag actttttcct cacctaggaa
     2821 acctatccca tgcctgtcac ttatagttca ggaggaagtt tttgcacaga ccagagagaa
     2881 attaaaatta gatgataaat tgaagatatc ttacacacag ttttttggtg aacactgatt
     2941 ttattggtgt cttagatccc tagtctaccc aaataatttt aacagtactg ttttttctaa
     3001 tcctgaagtc tgatatttat gactcattag caggaatcaa aactagtgat cagtagaaca
     3061 ctttcaaaat aaaaatttgg aatgcagact tttatgaaaa tttaaaagtg ctccttaaca
     3121 gaatatcatg ggttttccta taaaacttct ttaagtattg taattccagt ctgccccaac
     3181 tttaaaaaaa attcttatta atatgtcagt cattaattgc tagtttgggc tctcattatt
     3241 tcctgttttt taacaatttt gtgataattt tattattggc aaattaatac atcaacactt
     3301 aaatcattga ctataataat accttctggc tacctctgta tcaaccaaat tctgtaggtg
     3361 caaacatata ccagggaatt cttactggca aaatgatcaa tctggagtgt gcatccactg
     3421 tgaatggagc aaattgccct atacccattg ataacctagc tttcttagtt tgtagatgta
     3481 ggaaacaaaa tagtgacaga gagagaaggg ggtccacagg gcatggtata tttatcagca
     3541 gtggaaaaaa agtgcataga tcatttagtc caagaactta aaactaaata gagccataat
     3601 ttactttgga gagtcatttt aatttgtctt tggtaccaag gagaagacgg aaccaaaaac
     3661 aaactctcca agtatattca cacattcaac aaaatttttg catgccttct atgtcgtagg
     3721 catttttagt tcctggggat ttggacatgg ctaagtcaga gaaggccatt gctcaccatg
     3781 aacactgtat accagaagga gagtggggag gagacaaaaa acaaataaga ccacttcaga
     3841 caatcaaagt atcagttaag agaatgaaaa caggcctgac tcagtggctc acgcctgtaa
     3901 tcccagtact ttgggaggcg gaggttgggg gatcacctga ggtcaggaga tcgagaccag
     3961 cctggacaac atggtgaaaa cccgtctcta ctaaaaatac aaaaattaac tgggcatggt
     4021 ggcaggcacc tgtaatccca gctactgggg aagctgaggc aggagaatcg tttgaacctg
     4081 ggaggcggag gttgcagtga gccaagattc tgccactgca ctccagcctg ggtgacagtg
     4141 cgagactcca tctcaacatc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4201 agaataaaaa cagggtaaca taatgcaaag taactgtgtg gaataaaaat tgattatttt
     4261 agaaaatgtg actggcttag gacggggata atatgtgaac agaaatctat ctcatgagaa
     4321 agtgctactg ttgtcaaaat taccttatct gagtgaatgg tatttttttt atcttttcca
     4381 cacatgcgtg ggaaaggtat gatttctgca tgtaattgca gtttaaccct tatttctagg
     4441 ttgatcatag gtcccagttt acccaggaaa attccagttt atacctgttg tacctgtgta
     4501 attattggta gcactccctt tcactcttac aatgtcttgg tttggatgat atatggtgaa
     4561 gtttttgttg aaactaaatt atgaagtctg atatatttgg ataaaaataa agaattgctt
     4621 ttcttctc
//
