LOCUS       NM_004975              11871 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens potassium voltage-gated channel subfamily B member 1
            (KCNB1), mRNA.
ACCESSION   NM_004975
VERSION     NM_004975.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Lacroix,G., Bhat,S., Shafia,Z. and Blunck,R.
  TITLE     KCNG4 Genetic Variant Linked to Migraine Prevents Expression of
            KCNB1
  JOURNAL   Int J Mol Sci 25 (16), 8960 (2024)
   PUBMED   39201645
  REMARK    GeneRIF: KCNG4 Genetic Variant Linked to Migraine Prevents
            Expression of KCNB1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   Fernandez-Marino,A.I., Tan,X.F., Bae,C., Huffer,K., Jiang,J. and
            Swartz,K.J.
  TITLE     Inactivation of the Kv2.1 channel through electromechanical
            coupling
  JOURNAL   Nature 622 (7982), 410-417 (2023)
   PUBMED   37758949
  REMARK    GeneRIF: Inactivation of the Kv2.1 channel through
            electromechanical coupling.
REFERENCE   3  (bases 1 to 11871)
  AUTHORS   Sun,Q., Liu,F., Zhao,J., Wang,P. and Sun,X.
  TITLE     Cleavage of Kv2.1 by BACE1 decreases potassium current and reduces
            neuronal apoptosis
  JOURNAL   Neurochem Int 155, 105310 (2022)
   PUBMED   35231557
  REMARK    GeneRIF: Cleavage of Kv2.1 by BACE1 decreases potassium current and
            reduces neuronal apoptosis.
REFERENCE   4  (bases 1 to 11871)
  AUTHORS   Bar,C., Breuillard,D., Kuchenbuch,M., Jennesson,M., Le Guyader,G.,
            Isnard,H., Rolland,A., Doummar,D., Fluss,J., Afenjar,A.,
            Berquin,P., De Saint Martin,A., Dupont,S., Goldenberg,A.,
            Lederer,D., Lesca,G., Maurey,H., Meyer,P., Mignot,C., Nica,A.,
            Odent,S., Poisson,A., Scalais,E., Sekhara,T., Vrielynck,P.,
            Barcia,G. and Nabbout,R.
  TITLE     Adaptive behavior and psychiatric comorbidities in KCNB1
            encephalopathy
  JOURNAL   Epilepsy Behav 126, 108471 (2022)
   PUBMED   34915430
  REMARK    GeneRIF: Adaptive behavior and psychiatric comorbidities in KCNB1
            encephalopathy.
REFERENCE   5  (bases 1 to 11871)
  AUTHORS   Liu,M., Zhu,Y., Huang,L., Jiang,W., Wu,N., Song,Y., Lu,Y. and Ma,Y.
  TITLE     Compound heterozygous KCNV2 variants contribute to cone dystrophy
            with supernormal rod responses in a Chinese family
  JOURNAL   Mol Genet Genomic Med 9 (10), e1795 (2021)
   PUBMED   34535971
REFERENCE   6  (bases 1 to 11871)
  AUTHORS   Post,M.A., Kirsch,G.E. and Brown,A.M.
  TITLE     Kv2.1 and electrically silent Kv6.1 potassium channel subunits
            combine and express a novel current
  JOURNAL   FEBS Lett 399 (1-2), 177-182 (1996)
   PUBMED   8980147
REFERENCE   7  (bases 1 to 11871)
  AUTHORS   Hugnot,J.P., Salinas,M., Lesage,F., Guillemare,E., de Weille,J.,
            Heurteaux,C., Mattei,M.G. and Lazdunski,M.
  TITLE     Kv8.1, a new neuronal potassium channel subunit with specific
            inhibitory properties towards Shab and Shaw channels
  JOURNAL   EMBO J 15 (13), 3322-3331 (1996)
   PUBMED   8670833
REFERENCE   8  (bases 1 to 11871)
  AUTHORS   Melis,R., Stauffer,D., Zhao,X., Zhu,X.L., Albrecht,B., Pongs,O.,
            Brothman,A. and Leppert,M.
  TITLE     Physical and genetic localization of a Shab subfamily potassium
            channel (KCNB1) gene to chromosomal region 20q13.2
  JOURNAL   Genomics 25 (1), 285-287 (1995)
   PUBMED   7774931
REFERENCE   9  (bases 1 to 11871)
  AUTHORS   Albrecht,B., Lorra,C., Stocker,M. and Pongs,O.
  TITLE     Cloning and characterization of a human delayed rectifier potassium
            channel gene
  JOURNAL   Recept Channels 1 (2), 99-110 (1993)
   PUBMED   8081723
REFERENCE   10 (bases 1 to 11871)
  AUTHORS   Ikeda,S.R., Soler,F., Zuhlke,R.D., Joho,R.H. and Lewis,D.L.
  TITLE     Heterologous expression of the human potassium channel Kv2.1 in
            clonal mammalian cells by direct cytoplasmic microinjection of cRNA
  JOURNAL   Pflugers Arch 422 (2), 201-203 (1992)
   PUBMED   1283219
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035685.21, L02840.1,
            AF026005.1 and H12055.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004975.3.
            
            Summary: Voltage-gated potassium (Kv) channels represent the most
            complex class of voltage-gated ion channels from both functional
            and structural standpoints. Their diverse functions include
            regulating neurotransmitter release, heart rate, insulin secretion,
            neuronal excitability, epithelial electrolyte transport, smooth
            muscle contraction, and cell volume. Four sequence-related
            potassium channel genes - shaker, shaw, shab, and shal - have been
            identified in Drosophila, and each has been shown to have human
            homolog(s). This gene encodes a member of the potassium channel,
            voltage-gated, shab-related subfamily. This member is a delayed
            rectifier potassium channel and its activity is modulated by some
            other family members. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L02840.1, SRR3476690.1101695.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371741.6/ ENSP00000360806.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AL035685.21        149471-149629       c
            160-649             L02840.1           158-647
            650-2765            AF026005.1         462-2577
            2766-3297           L02840.1           2764-3295
            3298-11706          AL035685.21        31003-39411         c
            11707-11871         H12055.1           10-174              c
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..11871
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /note="potassium voltage-gated channel subfamily B member
                     1"
                     /db_xref="GeneID:3745"
                     /db_xref="HGNC:HGNC:6231"
                     /db_xref="MIM:600397"
     exon            1..755
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..2765
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /note="delayed rectifier potassium channel 1;
                     voltage-gated potassium channel subunit Kv2.1; potassium
                     voltage-gated channel, Shab-related subfamily, member 1"
                     /codon_start=1
                     /product="potassium voltage-gated channel subfamily B
                     member 1"
                     /protein_id="NP_004966.1"
                     /db_xref="CCDS:CCDS13418.1"
                     /db_xref="GeneID:3745"
                     /db_xref="HGNC:HGNC:6231"
                     /db_xref="MIM:600397"
                     /translation="MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEV
                     LWRTLDRLPRTRLGKLRDCNTHDSLLEVCDDYSLDDNEYFFDRHPGAFTSILNFYRTG
                     RLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREG
                     EEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLD
                     EFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVT
                     IFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLL
                     ILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKI
                     VGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNM
                     KDAFARSIEMMDIVVEKNGENMGKKDKVQDNHLSPNKWKWTKRTLSETSSSKSFETKE
                     QGSPEKARSSSSPQHLNVQQLEDMYNKMAKTQSQPILNTKESAAQSKPKEELEMESIP
                     SPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLTSLPSKTGGSTAPEV
                     GWRGALGASGGRFVEANPSPDASQHSSFFIESPKSSMKTNNPLKLRALKVNFMEGDPS
                     PLLPVLGMYHDPLRNRGSAAAAVAGLECATLLDKAVLSPESSIYTTASAKTPPRSPEK
                     HTAIAFNFEAGVHQYIDADTDDEGQLLYSVDSSPPKSLPGSTSPKFSTGTRSEKNHFE
                     SSPLPTSPKFLRQNCIYSTEALTGKGPSGQEKCKLENHISPDVRVLPGGGAHGSTRDQ
                     SI"
     exon            756..11871
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11849..11854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /note="hexamer: AATAAA"
     polyA_site      11871
                     /gene="KCNB1"
                     /gene_synonym="DEE26; DRK1; Kv2.1"
                     /note="major polyA site"
ORIGIN      
        1 gctttcgctt tctgcctcgc agccgtcggg atggaggtga gaagacggcc gtgacgcgcg
       61 cccgcggggc cccctgcacc cccagcagcc cacagcgctc cctgcccccc tcccccgcag
      121 cagcgggcct tgccgtcgag tgacagcggc ctgggggggc agggggggcg ggggcggccg
      181 gatcagcgat gccggcgggc atgacgaagc atggctcccg ctccaccagc tcgctgccgc
      241 ccgagcccat ggagatcgtg cgcagcaagg cgtgctctcg gcgggtccgc ctcaacgtcg
      301 gggggctggc gcacgaggta ctctggcgta ccctggaccg cctgccccgc acgcggctgg
      361 gcaagctccg cgactgcaac acgcacgact cgctgctcga ggtgtgcgat gactacagcc
      421 tcgacgacaa cgagtacttc tttgaccgcc acccgggcgc cttcacctcc atcctcaact
      481 tctaccgcac tgggcgactg cacatgatgg aggagatgtg cgcgctcagc ttcagccaag
      541 agctcgacta ctggggcatc gacgagatct acctggagtc ctgctgccag gcccgctacc
      601 accagaagaa agagcagatg aacgaggagc tcaagcgtga ggccgagacc ctacgggagc
      661 gggaaggcga ggagttcgat aacacgtgct gcgcagagaa gaggaaaaaa ctctgggacc
      721 tactggagaa gcccaattcc tctgtggctg ccaagatcct tgccataatt tccatcatgt
      781 tcatcgtcct ctccaccatt gccctgtccc tcaacacgct gcctgagcta cagagcctcg
      841 atgagttcgg ccagtccaca gacaaccccc agctggccca cgtggaggcc gtgtgcatcg
      901 catggttcac catggagtac ctgctgaggt tcctctcctc gcccaagaag tggaagttct
      961 tcaagggccc actcaatgcc attgacttgt tggccattct gccatactat gtcaccattt
     1021 tcctcaccga atccaacaag agcgtgctgc aattccagaa tgtccgccgc gtggtccaga
     1081 tcttccgcat catgcgaatt ctccgcatcc ttaagcttgc acgccactcc actggcctcc
     1141 agtctctggg cttcactttg cggaggagct acaatgagtt gggcttgctc atcctcttcc
     1201 ttgccatggg cattatgatc ttctccagcc ttgtcttctt tgctgagaag gatgaggacg
     1261 acaccaagtt caaaagcatc ccagcctctt tctggtgggc caccatcacc atgactactg
     1321 ttgggtatgg agacatctac cccaagactc tcctggggaa aattgttggg ggactctgct
     1381 gcattgcagg agtcctggtg attgctcttc ccatccccat catcgtcaat aacttctctg
     1441 agttctataa ggagcagaag agacaggaga aagcaatcaa acggcgagag gctctggaga
     1501 gagccaagag gaatggcagc atcgtatcca tgaacatgaa ggatgctttt gcccggagca
     1561 ttgagatgat ggacattgtg gttgagaaaa atggggagaa tatgggtaag aaagacaaag
     1621 tacaagataa ccacttgtct cctaacaaat ggaaatggac aaagaggaca ctgtctgaaa
     1681 ccagctcaag taagtccttt gaaaccaagg aacagggatc ccctgaaaaa gccagatcgt
     1741 cttctagtcc tcagcacctg aacgttcagc agttggaaga catgtacaat aagatggcca
     1801 agacccaatc ccaacccatc ctcaatacca aggagtcagc agcacagagc aaaccaaagg
     1861 aagaacttga aatggagagt atccccagcc ccgtagcccc tctgcccact cgcacagaag
     1921 gggtcattga catgcgaagt atgtcaagca ttgatagttt cattagctgt gccacagact
     1981 tccctgaggc caccagattc tcccacagcc ctttgacatc actccccagc aagactgggg
     2041 gcagcacagc cccagaagtg ggctggcggg gagctctggg tgccagtggt ggtaggtttg
     2101 tggaggccaa ccccagccct gatgccagcc agcactctag tttcttcatc gagagcccca
     2161 agagttccat gaaaactaac aaccctttga agctccgagc acttaaagtc aacttcatgg
     2221 agggtgaccc cagtccactc ctccccgttc tagggatgta ccatgaccct ctcaggaacc
     2281 gggggagtgc tgcggctgct gtcgctggac tggagtgtgc cacgcttttg gacaaggctg
     2341 tgctgagccc agagtcctcc atctacacca cagcaagtgc taagacaccc ccccggtctc
     2401 ctgagaaaca cacagcaata gcgttcaact ttgaggcggg tgtccaccag tacattgacg
     2461 cagacacaga tgatgaggga cagctgctct acagtgtgga ctccagcccc cccaaaagcc
     2521 tccctgggag caccagtccg aagttcagca cggggacaag atcggagaaa aaccactttg
     2581 aaagctcccc tttacccacc tcccctaagt tcttaaggca gaactgtatt tactccacag
     2641 aagcattgac tggaaaaggc cccagtggtc aggaaaagtg caaacttgag aaccacatct
     2701 cccctgacgt ccgtgtgttg ccagggggag gagcccatgg aagcacacga gatcagagca
     2761 tctgaactgc cctgccttgg aggagagact tttgggtgag gtccaaagag gagagctgtt
     2821 cagcttacct gccacagagc ttttctgcat gaactctgga acagaaaggc cctgtaaagc
     2881 cctcagagag aagagagact ccagagaagg ctccctaaga ccttgagagc catgacaggt
     2941 ccatcagcat gaagttggcc aagccatagg gcacagcacc tccttgtaac aactctatag
     3001 ccctctttgg gagatgacat gagtggaact cacagccacc actaccacca ctttagacag
     3061 gaccgaggcc acatactccc cattctctcg tggctttcca tctcagcctc ggagggcaac
     3121 attgacagtc ctcctggctt cagctagaga aggatgctgg aacaagcggc tggtgttgaa
     3181 agagtgggtt gaccaatttg gtattgaatg ttgcccagcc acccctagga acacctgtcc
     3241 atcacctcct ggatggattc cactgttaga cagctacagg gaatgattgg tcatggggaa
     3301 gtctctgcgc cataagccac gatcccagcg caaaaccctt actcaaatgt cttcattgac
     3361 ttcggtattt catagtacct gagattttat tttgagatac catcagggtg agttgcacca
     3421 cttgtactca attctaattg ccccctggca atctgggaag ggttcagaag gtgggcaccc
     3481 agccaacagc atgaactcag agcattgttt tagggttgga ggaggaacac gctttcttta
     3541 catcactagt gtagactcaa aagatatgca agtgtcaaat atgcaaaaga aatagtttat
     3601 tcaaagagac tgtgtgttac tgaagaacag cataaaaata tgattttttt acttgcaaaa
     3661 atgaaaggaa aaaaatacca cgcattgaaa tgcccagttc agactgaata attcctgctg
     3721 cagcaaggaa agtacctact ataatagaaa ttctgttttg ttttctgtgg ttttcaagtt
     3781 aaaaaaaaaa aagttaaaat aaaaagcact ttatgttttt caaaaatagg ttctgccagg
     3841 gacagtgtca acatcttgca ctttaaaata agcactattt cccatagccc aattgttggg
     3901 actgtagttg gattgttgct acctgcccat ttctggccag catgggtaat attgtgctgg
     3961 tattactgtc tgccatatca aagtctgtgg gctccttccc tgcccctctg aaaccttccc
     4021 ttaaccctca tgggtcagga ggggttcttg ccccttgggt ggggcctctc atgaagtcaa
     4081 gtatacagga gacatgcaca tctcaaaagg acacagtgac agttggacat tgggtgtttt
     4141 actctgttct ctttcttgtc aaccagctat tggtttttca gtgtcactgt ctgtactaaa
     4201 agctcaactc tccttcctgc agccatcgat gcagccagta cagacaaaag caataagtat
     4261 ttgtgtgcat cctgagtggt tctggtgtat ttcttggtgt tggttttcct gtcctcgttt
     4321 gatgacctgg cattgtcctg cctgctttta gtccaacagc atggtgttgt tggccattcc
     4381 tcagtacagt ggcattaggt tgaaccactg atgaatacaa tgctatagtg ttggctcacc
     4441 tgtcagtaaa gatgacatgt tattggtctg cctgtctgta gtacaatgga acaatgttgt
     4501 tctcttaatg taatgttgat ggatgatgtc ccaagtacaa tagtgtagtt ctgtctatcc
     4561 tcaatacagt aacaggcata tgtcttccca gtggaagaaa gatatggtat tggttcacct
     4621 agtcttaagt ctcaatatca tctgttgctg gtctgcctgt tagtacaatg gcatgatcca
     4681 cctctcagta cacaaatatc atggagctgt tctgcctgct cttcatacat aaggcataag
     4741 gttgagccac ctgccacaca tatgatgaga tgggatcatt ccaccaccct taaaaatatg
     4801 tcttctctgc tgtatacaaa cccaattcat aacacttctc agcatctcca gagactgtag
     4861 atccccccaa gagggtgcag tcctgttctt tgccgcatta acagagaaaa cgagaacgtc
     4921 actcttccta ggtatcttca gagtattcgc gcagcaactg caaagggact taggggcagg
     4981 gagagtgtga ggtcatcccg tgccaggcag aggaagcagc actcccaaaa cactttgctg
     5041 aacacgcagt ctgagctggg ggcctcttga ggaatgactg tgggacagtt ctccgccgac
     5101 catctcacat gtctcttctt tgttttcatc accattaatg tccaccatca gcacaaatgt
     5161 gaaaattcag ggaaaaaaac aaatgctttt tgataaaagt aaatagttgt gatttccaat
     5221 tcagatattt ttagagctga tgctatctgt aaaaaataat aataataata atatagtcta
     5281 ttttacatat caggaaagtg aacatgtttc aatatagcca tatatattga gaaggaactt
     5341 accacccctt cttccaatcg aggcatttca tttgttggtt gaagcagatg agaactgtac
     5401 agattgaaaa tgatttgttt tttttttttt ttaaaaaaaa cgaaaaccaa ttggtgcaac
     5461 tctccttgta accaaaggcc ctttctgcct tctgtggtca tgtaggacct ggcccttcct
     5521 tccttgtgta ttttgtccaa ccgtaaagta gcattttatt gagtcacttt tgaaggacga
     5581 attcagaagt atcattaaga acctaccttt tcaggcttca tagccagagt cctatagagt
     5641 tcttatagaa acagaataat tgaaattcag catatactat gcttagaaag tattttgttt
     5701 gggtttgatt gggttttttt ttcttttact tgtgtgtgta tgtactgggg gagtatagct
     5761 tttattctgt gctcaattcg aaagagagta ttataagttt tcccagccca tccacccgct
     5821 aaaaaagaac atatccaaat tttaatatgt atacacccaa cctgtaccta tgtacgtgaa
     5881 tatacatgca ctgggaaatc tacttaagag tcttcactga gaagttcact cgatagaaag
     5941 gtcttgatag agaagagaag aggaaagcaa tggtctgaag gcaaatcctg ctttaacttg
     6001 gtggtccatt tcccattcat tcctgaacca atgagagcag aaagaaacaa gtcaacccaa
     6061 acaagcaaac taggggctgg acgcggcgaa tggatgctgg tcagtttggc aaattcactg
     6121 aaactcatca agaacccagc tgaacttgca ttccaacttc cacaattatc tgaaagggct
     6181 agggaccagg tagccatgtg tcccaatcac acgggggaga gtctgccacc ggcggtgaga
     6241 gaggtgggga agctttggag gagacagtat attcaccttt ttatctttgg gtaaaagaaa
     6301 ccaacaggac tttggtgcag tagatttctc agtgccttct ttaggaattc tatcttaagc
     6361 acacttcaag gggaaagaaa aaaggaggag agaacatttg ttcccgtgaa gtccctttct
     6421 ccaaaggact ggggatcaag aaggaagaag agtaagaagg aaaaacaaca aaatcctgag
     6481 gaggccattt cttttaaccc acccagcttg cccctgaaaa ctgctggtga tacaaacttt
     6541 ttcaaactga cttccagctt ctccatgtgt cttggtaact gtagcctcgt ctctgccctt
     6601 tctcctaata agatccatgt ttctaaggag aaaaacaaaa cagtattctc ttctgtaaaa
     6661 ctgtaatgta ttgttgatat ttgtaactat tgtatatttc tgtcttgctc cagtcccagc
     6721 tggagtgtct agttccatgg acaaggggat atttaaaatt caccacggac aagagccact
     6781 aaagccaggt ctctatcgct aatctctctc ctattcttat ttctctacca actaatctca
     6841 agtaacaata ccccaggctt tttttcagcc tcaattcaag taactttgaa tcaaaagaga
     6901 gcaaggcaaa aagcctccgg acatgcttct ttgggaaagg actttttctg tagctgcaga
     6961 gtgggcagga gggagaaggg gctcccctga gaccccggca ggacaatgga aagcttccag
     7021 ccccgagctg atgtttcctc acctatcact gcttatagga cagcatccaa atgctttcat
     7081 gtcactcagc ccaggcaacc caaaccagtc ctggctacac ccagaaataa gagttacctc
     7141 tgcattttct gttgccattt ttctggctct agaagtatct gatgcgcgac tatgtcatag
     7201 aatgtccacg catctcagag tagtctattt ttctgttcag ataaaatata tatatatatg
     7261 tatttttagc aaatttataa tagggcaatt gagtctaatt tcttcttttg taatacagaa
     7321 caatatctta ggagtttcat tgttggtcat tatcgtagct gatgttttct ggtcttttgc
     7381 agttcctacc attgtccctt gggctgtttg ggttttcttc cttttgattc tgatttcttg
     7441 agggtgggga aagactttgg ggtttgactt ccttgtgtag gcatttcttg acactaggcc
     7501 tggactctgt caatgctgtg actttcctca ttcatttctg tgtgtttctc ccattcgttg
     7561 ttcttaagaa tccagggcat gaatgcagga atgagaccca gggaagcaga gctacagatg
     7621 gccctcaaaa tcaggatggg atgtgggctg gtttcactta tatcccacac ccctttaggg
     7681 atctgaaggg actgagtttt ctcatcccca gagatcttgg aaccaaaaat ctcatgcaag
     7741 caaactcatc actggcattt ctgagatcca gaacacatgt gagtccctcc tggctgggct
     7801 gctcccacat gtccagcacc ctgtcggcct gtaacatcaa tcagagctga aggtcagagg
     7861 tttttcaggt tctcgtgaac ctcacaagaa catgctgggc ctccgatctc accaacttgt
     7921 ttatcagttc cttgtttcga tctgtttatt tatttatttt tttgttatta ttattttgag
     7981 acaaagtctt gctctgttgc ccaggctgca gtgcaatggc gcaatctcgg ctcactgcaa
     8041 cctcctccac ccaggttcaa gtgattctca tgtctcagcc tcctgagtaa ctgggattac
     8101 aagagcgcac catcacgccc agctaatttt tgtattttta gtagaggcag agtttctcca
     8161 tgttggccag gctggtcttg aactcctgac ctcaggtgat ctgcctgcct cggcctccca
     8221 gtgctaggat tacaggtgtg agccaccact gcacccagtc catttcaatc catttctaac
     8281 agcttcacca aaaaacagtg gccagacacc cttgcagcca aaccctgaga agagatgtcc
     8341 ccgaatctgc tagctcatct ccccttccct tgccagctgc ctggctccat agagcaggag
     8401 gtgtgtggca aggacagggg cagttttcca gcttccacct atccacgcct gggggagttt
     8461 ttggactgtc ctgataatta attccagagg cagttttcag tcctgggtgg gcggaggaca
     8521 tctcactagg caggatcttc agcagaaagt gattctccat ccgtcaactc atgcaggaag
     8581 atgaagaaag cccagataat ctccaacttg cattcgttta ggttttggta taccacccac
     8641 ctcactgtca gctgcagggc ttggggctct gcctagaatc agaaatttga gccaaactgg
     8701 gaccctcaga tggcagtgag ccatcccatc agataactcc caagaggaag ccaactcaaa
     8761 tatgaatttt attattctgg actatgaccc tgatcagagt tctcccaccc cgaaaacctc
     8821 tgatagtctg gtttgaacca gagtcaaata aaacagacaa gatttttttt tcctctgcac
     8881 aaaagcaaaa aaacaaaaca aaaaaaagtc tgccagtagt ttcctgggtt tgatgcccag
     8941 ataagcaaac actgttgcct atggctgttg ctgttttcat caagaaagag gttatatagt
     9001 ttgcatttcc atccactctc cctgatttta tcttaaccta gaatttctcc tgaactaccc
     9061 caaatgccaa cttgaatttg ggtagggctc tgttaatcga gggaaccctc atcttcactt
     9121 ataaacctaa ttctctgttc ctccctccaa agatcctgaa aagatctcct aaaggctggc
     9181 caggatgggg tggagtgggg cagtgcacct gaagaaaaaa acttagctgc cagctggctg
     9241 tatgcaagtt ctccgaaaat ttctattgta tgaaagggat ggggtcaatt gcagctcaaa
     9301 ttaaggaagc cctcctaaca ggctgggttg tctgaccatg gagctcaatg ccatggtgga
     9361 cagtgagctc ccttatatca caggtgttta aagagaggct ggctagatgg tgtaaaaaat
     9421 gggcattgca gaggggccct agcattgcat ggaaactggg ctgaacaacc acagaaattc
     9481 ctctttgttt cttggagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     9541 aggtggtttc aacactctgg atataagctc aagccactga ggcaaaggca tgcagcacaa
     9601 ttagcaggaa tgcaacttct gggctttgga gctctcagag ggggtgtcca agagacaagt
     9661 ctgcttcctt ccggctccgt cttggaacat tttaatgact gtcagtcacc aatccaaccc
     9721 atccccagtg gcctaccagg caccacattg ggatgagact catgctaccc ttccccaggc
     9781 cagccaagga gcaatccacg ctgtccccca tccctggctg gctgtcttat ggtgcttcag
     9841 cccctccgcc cagctcctgt gccctcatga gcctggggct ttctgtataa aagatgcaat
     9901 gatatctgtt ccccactagc catccccaca atgaacaact tcccaggcaa tagcaaatgg
     9961 ctccattcca aaagggcttg tttttttggt aaatcaaata agagacccca ctcaagacct
    10021 ttgaaagtcc ccgtcaatga aaaaaatcca gaagggtcaa aagagaaagg cagcttttaa
    10081 tccagtttca cagcaaaacc aggctcttgg ctgcctccaa gcagaatgcc actctcctgc
    10141 agctgtggcc tccatcccaa atcatcctgc atactgcatt ctctaaggaa agcatttgac
    10201 aagagagggt ttatgtatgg tggattggga gaaagtgaga cttcaacctc agggtgaacc
    10261 gaagactggt ttgtctttgg ttgttttagt atgaggttgg gcaggatgat atgtttggat
    10321 gggtagaatg agcagccgcc ttcctctgaa accaaaataa aataaaaacc agttttcaag
    10381 attcctccca actcctcact ggaggctctg ccctgcagaa tcactcaagt tgcaacaaca
    10441 caaggagaca aaaataggat atttcatgaa aatataagaa actcccaatt ccgtcccatt
    10501 aggatgccct caaggggcca tgcagggatc ttcccatact tcctgcttcc ctgatctgaa
    10561 ccaagattgg aaagaggcag gcagcagaga agatgcccaa gaaactctga catatccaca
    10621 gcttggctct gccccttggg cttcactggg gtttgggcct ccctcttggt tgagctgagt
    10681 gtgcctgagt gggtggggac agggaggatt ctccaggtct atcttgattt cataggtcct
    10741 tctcaatcca aggtcaaaaa ctgagaatct tattttctgc accaggaact cagggttatc
    10801 cccaacctat gccgctggca catgatggtc atccaacctg tgtttttgtg agtgagtgaa
    10861 tgtgtgactg gttggcctct gagtggatga ctggatgcgt gaatgtgtga gtgaatgggg
    10921 gagttaatga atccctacct aactgtgaag agaagaaaaa tgaggagccc ctctcccact
    10981 gaaacccata actgccccat caaggtcatc tttctttgac tcaagatgac atctcaattt
    11041 gatgcttgta ggccccaagc tgataagggc cacagccctc tcagagccac acttcctggg
    11101 ggcccacaga gcagctctgc cctccttcct cttcccctac cctgcatttc tcagcctcat
    11161 gttctatttt agctcacaca cgacccatgg gggggaccac caagtggttt tccaatggac
    11221 taagccaagg gttcgaagtt gggacttcct ttggtctggg acttcctggg gcagaaacat
    11281 aggagccaac atctggaaac tggattgcat tccctgagcc atgggagtcc agctggatat
    11341 taaggccagc ctgccctcca gcttttagag ccgaagatct ttttttctga gccccaaagc
    11401 cgtcatgata gtatgccccc agatcccatc agctaagacc ctgtctctcc caggaacccg
    11461 tcgtgtgagg ttggggcttt tctgccagta aagccattct gatcacctca cccttgacag
    11521 gggccaagcc aatcacatta gccataaatg accaatgtcc tcagccactc ttttctcggg
    11581 ggtgcattcg tgggtcctat tcatctctcg tcagttaatg tctctagtcc ttcctgtaaa
    11641 ggtgaaaata agcattgcgc atgtgcgcaa cacacacgca cacacacaca cacacacaca
    11701 cacacaaatc ccagtgtctt tttatgcatg tggtgagatg aaattgtgat ctttgtgtga
    11761 cctccagctg ctgtcttgtc ttgtaaaata tgtccaaatg tttaagaatt tctaagcaaa
    11821 tggtccctta atgaagtctg cagagttcaa taaaaggtat ggaaaaaaca a
//
