LOCUS       NM_020433               9502 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens junctophilin 2 (JPH2), transcript variant 1, mRNA.
ACCESSION   NM_020433
VERSION     NM_020433.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Shi,Y., Mirabdali,S., Vetter,S.W. and Guo,A.
  TITLE     Junctophilin-2 is a double-stranded RNA-binding protein that
            regulates cardiomyocyte-autonomous innate immune response
  JOURNAL   Biochem Biophys Res Commun 733, 150725 (2024)
   PUBMED   39317111
  REMARK    GeneRIF: Junctophilin-2 is a double-stranded RNA-binding protein
            that regulates cardiomyocyte-autonomous innate immune response.
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Patel,V. and Chahal,C.A.A.
  TITLE     Editorial commentary: Genomic and precision medicine provides
            deeper insights into the genetic basis of diverse JPH2-mediated
            phenotypes
  JOURNAL   Trends Cardiovasc Med 33 (1), 11-12 (2023)
   PUBMED   34965474
  REMARK    GeneRIF: Editorial commentary: Genomic and precision medicine
            provides deeper insights into the genetic basis of diverse
            JPH2-mediated phenotypes.
REFERENCE   3  (bases 1 to 9502)
  AUTHORS   Parker,L.E., Kramer,R.J., Kaplan,S. and Landstrom,A.P.
  TITLE     One gene, two modes of inheritance, four diseases: A systematic
            review of the cardiac manifestation of pathogenic variants in
            JPH2-encoded junctophilin-2
  JOURNAL   Trends Cardiovasc Med 33 (1), 1-10 (2023)
   PUBMED   34861382
  REMARK    GeneRIF: One gene, two modes of inheritance, four diseases: A
            systematic review of the cardiac manifestation of pathogenic
            variants in JPH2-encoded junctophilin-2.
            Review article
REFERENCE   4  (bases 1 to 9502)
  AUTHORS   Perni,S. and Beam,K.
  TITLE     Junctophilins 1, 2, and 3 all support voltage-induced Ca2+ release
            despite considerable divergence
  JOURNAL   J Gen Physiol 154 (9) (2022)
   PUBMED   35089322
  REMARK    GeneRIF: Junctophilins 1, 2, and 3 all support voltage-induced Ca2+
            release despite considerable divergence.
REFERENCE   5  (bases 1 to 9502)
  AUTHORS   Yang,Z.F., Panwar,P., McFarlane,C.R., Tuinte,W.E., Campiglio,M. and
            Van Petegem,F.
  TITLE     Structures of the junctophilin/voltage-gated calcium channel
            interface reveal hot spot for cardiomyopathy mutations
  JOURNAL   Proc Natl Acad Sci U S A 119 (10), e2120416119 (2022)
   PUBMED   35238659
  REMARK    GeneRIF: Structures of the junctophilin/voltage-gated calcium
            channel interface reveal hot spot for cardiomyopathy mutations.
REFERENCE   6  (bases 1 to 9502)
  AUTHORS   Matsushita,Y., Furukawa,T., Kasanuki,H., Nishibatake,M.,
            Kurihara,Y., Ikeda,A., Kamatani,N., Takeshima,H. and Matsuoka,R.
  TITLE     Mutation of junctophilin type 2 associated with hypertrophic
            cardiomyopathy
  JOURNAL   J Hum Genet 52 (6), 543-548 (2007)
   PUBMED   17476457
  REMARK    GeneRIF: this is the first report on JPH2 mutation associated with
            hypertrophic cardiomyopathy
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 9502)
  AUTHORS   Minamisawa,S., Oshikawa,J., Takeshima,H., Hoshijima,M., Wang,Y.,
            Chien,K.R., Ishikawa,Y. and Matsuoka,R.
  TITLE     Junctophilin type 2 is associated with caveolin-3 and is
            down-regulated in the hypertrophic and dilated cardiomyopathies
  JOURNAL   Biochem Biophys Res Commun 325 (3), 852-856 (2004)
   PUBMED   15541368
REFERENCE   8  (bases 1 to 9502)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 9502)
  AUTHORS   Nishi,M., Mizushima,A., Nakagawara,Ki. and Takeshima,H.
  TITLE     Characterization of human junctophilin subtype genes
  JOURNAL   Biochem Biophys Res Commun 273 (3), 920-927 (2000)
   PUBMED   10891348
REFERENCE   10 (bases 1 to 9502)
  AUTHORS   Cirino,A.L. and Ho,C.
  TITLE     Hypertrophic Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301725
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035447.6, BC029957.1,
            AL034419.22, BC043206.2 and AW014086.1.
            
            On Oct 16, 2019 this sequence version replaced NM_020433.4.
            
            Summary: Junctional complexes between the plasma membrane and
            endoplasmic/sarcoplasmic reticulum are a common feature of all
            excitable cell types and mediate cross talk between cell surface
            and intracellular ion channels. The protein encoded by this gene is
            a component of junctional complexes and is composed of a C-terminal
            hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum
            membrane and a remaining cytoplasmic domain that shows specific
            affinity for the plasma membrane. This gene is a member of the
            junctophilin gene family. Alternative splicing has been observed at
            this locus and two variants encoding distinct isoforms are
            described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2012114.1,
                                           SRR14372079.1525635.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2155550
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372980.4/ ENSP00000362071.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AL035447.6         23659-23749         c
            92-1118             BC029957.1         482-1508
            1119-1325           AL034419.22        148612-148818       c
            1326-4265           BC043206.2         6-2945
            4266-4395           AW014086.1         18-147              c
            4396-9502           AL034419.22        95257-100363        c
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..9502
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /note="junctophilin 2"
                     /db_xref="GeneID:57158"
                     /db_xref="HGNC:HGNC:14202"
                     /db_xref="MIM:605267"
     exon            1..862
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             484..2574
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     junctophilin type 2"
                     /codon_start=1
                     /product="junctophilin-2 isoform 1"
                     /protein_id="NP_065166.2"
                     /db_xref="CCDS:CCDS13325.1"
                     /db_xref="GeneID:57158"
                     /db_xref="HGNC:HGNC:14202"
                     /db_xref="MIM:605267"
                     /translation="MSGGRFDFDDGGAYCGGWEGGKAHGHGLCTGPKGQGEYSGSWNF
                     GFEVAGVYTWPSGNTFEGYWSQGKRHGLGIETKGRWLYKGEWTHGFKGRYGIRQSSSS
                     GAKYEGTWNNGLQDGYGTETYADGGTYQGQFTNGMRHGYGVRQSVPYGMAVVVRSPLR
                     TSLSSLRSEHSNGTVAPDSPASPASDGPALPSPAIPRGGFALSLLANAEAAARAPKGG
                     GLFQRGALLGKLRRAESRTSVGSQRSRVSFLKSDLSSGASDAASTASLGEAAEGADEA
                     APFEADIDATTTETYMGEWKNDKRSGFGVSERSSGLRYEGEWLDNLRHGYGCTTLPDG
                     HREEGKYRHNVLVKDTKRRMLQLKSNKVRQKVEHSVEGAQRAAAIARQKAEIAASRTS
                     HAKAKAEAAEQAALAANQESNIARTLARELAPDFYQPGPEYQKRRLLQEILENSESLL
                     EPPDRGAGAAGLPQPPRESPQLHERETPRPEGGSPSPAGTPPQPKRPRPGVSKDGLLS
                     PGAWNGEPSGEGSRSVTPSEGAGRRSPARPATERMAIEALQAPPAPSREPEVALYQGY
                     HSYAVRTTPPEPPPFEDQPEPEVSGSESAPSSPATAPLQAPTLRGPEPARETPAKLEP
                     KPIIPKAEPRAKARKTEARGLTKAGAKKKARKEAALAAEAEVEVEEVPNTILICMVIL
                     LNIGLAILFVHLLT"
     exon            863..1652
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1771
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..2493
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2588
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..9502
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4373..4378
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /note="hexamer: AATAAA"
     polyA_site      4395
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /note="major polyA site"
     regulatory      9482..9487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
                     /note="hexamer: ATTAAA"
     polyA_site      9502
                     /gene="JPH2"
                     /gene_synonym="CMD2E; CMH17; JP-2; JP2"
ORIGIN      
        1 gacagccccg cacacccact ggggtggaga agggggccag cgggctgcgg gaattcagcc
       61 tccggcgggg gctgccaccg aggggaattt cccctctttg aaaaggctgg ggacccctcc
      121 accccaccct ttcactgggc ttcttaaaga gatcacaccg ttttcccttg gacttcttgg
      181 aaggcagact tgggacgctt tgtggggact ttgaaccttt ccttcctgct ttctttccag
      241 caggcagcct tgctctggct ggtggagtcg gggctcccgg gcatggcact gactagccga
      301 cccggtgctg acttggcgcc ggggctcttt cagcctctgc tggcatcctg cttcccccct
      361 cctggggtgc agtggtgagt ctgggggcct gcccccgagg gcactggagg agtgttgcag
      421 gcatcaccca cgccctctgc acccacgctg gaggacgggg aggttgtcag gggctatgat
      481 gagatgagtg ggggccgctt cgactttgat gatggagggg cgtactgcgg gggctgggag
      541 gggggaaagg cccatgggca tggactgtgc acaggcccca agggccaggg cgaatactct
      601 ggctcctgga actttggctt tgaggtggca ggtgtctaca cctggcccag cggaaacacc
      661 tttgagggat actggagcca gggcaaacgg catgggctgg gcatagagac caaggggcgc
      721 tggctctaca agggcgagtg gacacatggc ttcaagggac gctacggaat ccggcagagc
      781 tcaagcagcg gtgccaagta tgagggcacc tggaacaatg gcctgcaaga cggctatggc
      841 accgagacct atgctgatgg agggacgtac caaggccagt tcaccaacgg catgcgccat
      901 ggctacggag tacgccagag cgtgccctac gggatggccg tggtggtgcg ctcgccgctg
      961 cgcacgtcgc tgtcgtccct gcgcagcgag cacagcaacg gcacggtggc cccggactct
     1021 cccgcctcgc cggcctccga cggccccgcg ctgccctcgc ccgccatccc gcgtggcggc
     1081 ttcgcgctca gcctcctggc caatgccgag gcggccgcgc gggcgcccaa gggcggcggc
     1141 ctcttccagc ggggcgcgct gctgggcaag ctgcggcgcg cagagtcgcg cacgtccgtg
     1201 ggtagccagc gcagccgtgt cagcttcctt aagagcgacc tcagctcggg cgccagcgac
     1261 gccgcgtcca ccgccagcct gggagaggcc gccgagggcg ccgacgaggc cgcacccttc
     1321 gaggccgata tcgacgccac caccaccgag acctacatgg gcgagtggaa gaacgacaaa
     1381 cgctcgggct tcggcgtgag cgaacgctcc agtggcctcc gctacgaggg cgagtggctg
     1441 gacaacctgc gccacggcta tggctgcacc acgctgcccg acggccaccg cgaggagggc
     1501 aagtaccgcc acaacgtgct ggtcaaggac accaagcgcc gcatgctgca gctcaagagc
     1561 aacaaggtcc gccagaaagt ggagcacagt gtggagggtg cccagcgcgc cgctgctatc
     1621 gcgcgccaga aggccgagat tgccgcctcc aggacaagcc acgccaaggc caaagctgag
     1681 gcagcggaac aggccgccct ggctgccaac caggagtcca acattgctcg cactttggcc
     1741 agggagctgg ctccggactt ctaccagcca ggtccggaat atcagaagcg ccggctgctg
     1801 caggagatcc tggagaactc ggagagcctg ctggagcccc ccgaccgggg cgccggcgca
     1861 gcgggcctcc cacagccgcc ccgcgagagc ccgcagctgc acgagcgtga gacccctcgg
     1921 cccgagggtg gctccccgtc accggccggg acgcccccgc agcccaagcg gcccaggccc
     1981 ggggtgtcca aggacggcct gctgagccca ggcgcctgga acggcgagcc cagcggtgag
     2041 ggcagccggt cagtcactcc gtccgagggc gcgggccgcc gcagccccgc gcgtccagcc
     2101 accgagcgca tggccatcga ggctctgcag gcaccgcctg cgccgtcgcg ggagccggag
     2161 gtggcgcttt accagggcta ccacagctat gctgtgcgca ccacgccgcc cgagccccca
     2221 ccctttgagg accagcccga gcccgaggtc tccgggtccg agtccgcgcc ctcgtccccg
     2281 gccaccgccc cgctgcaggc ccccacgctc cgaggccccg agcctgcacg cgagaccccc
     2341 gccaagctgg agcccaagcc catcatcccc aaagccgagc ccagggccaa ggcccgcaag
     2401 actgaggctc gagggctgac caaggcgggg gccaagaaga aggcgcggaa ggaggccgca
     2461 ctggcggcag aggcggaggt ggaggtggaa gaggtcccca acaccatcct catctgcatg
     2521 gtgatcctgc tgaacatcgg cctggccatc ctctttgttc acctcctgac ctgaccgtcg
     2581 cttaccagag ccagaagttc ctgctgagga catgaggacc cgtctttttg cagtgccagg
     2641 cagggtggcc aaagaggact ggactctgga cctggagccc cgtggtccag ttcacattca
     2701 ccccacaaga gtttcaggag gcctcccacg gtgcctgtgc tgggtggcgg tggtggtgcc
     2761 aagaggaatg gaatgtcctg ggctccttca ggagctctct acccaggggc aaggagagcc
     2821 cagagagaag cgccctggtc tcttgagctt cctgatctgc tcctgtcccc cgctctcctc
     2881 cactcccttg cctttcccta ggttgtcccc tccctgggct tttgtgtgtt ttgggagatg
     2941 tcacctaacc aggacattga tattcaatcc catccccctt cctcccaccc tgccccactt
     3001 tgatttaatc ctttggctgt gggctgaggc ctcccaggga agctgggtgg ggtgggtgtt
     3061 gagaccccct cagaccagca cagagacctg tccttgtgca gtctgcaccc tgcactccct
     3121 cccttgcctg tagatgttct ggatgacagt agaggaaatg gacaaggtca gtttgaatat
     3181 cccagaacac agtgctctgt ctcctcccac cagtccagtt agcttccctt ctggaccaat
     3241 agacgagggg agaccccatg gatcctctgg ctgggaagcc actgaccagg tggccagggg
     3301 gcagggtggg aagaggggtt aaggtgcagt gatgatggcc tgttttggag tgtgtctgag
     3361 actgggattg catttggggt ttcccgtgtg cttgggatgc tagagggtca cctgcaggag
     3421 gcctggggcc ggcgagaaat ctcctgtgat gccctgtgaa atggcttgtc tcctccccca
     3481 tcagggccca ccgaaagctc aggggagcac agaagcccat ggaagcccag ggagatgtcc
     3541 ctggggcaga cactaaggca ggtgttgaag acaagctgct tgtcaagaag catttcccgg
     3601 caagagaggg gcaagtctgg ggctccaact gggtacagcc tgggtgcagt tataagcccc
     3661 tttggcttac ttggtagaag atggctactt ggatgtacct cacttaaaga tgttttgtac
     3721 cacactaggt ctctgggccc ttgtgcttcc tgtgggtggg gtgagggcca aaggctatgg
     3781 tttcctgcct ccaggagaat ggagagaaag ggcttccagg cccctccaag cctggggaag
     3841 gacgtggcat ccaagctgag ccagagggaa ctgctgctgt cctcccttca tttctgtgga
     3901 ccttggaggc tttggctttg tggcagggcc tccccaggca gctctgggac ctaggagttt
     3961 gcttctgata gggtcagctt tcccatttcc cttcaatgct tgggaacctt ctcccttagc
     4021 ttcacacttg ccgtttcaag ccctgctggg accttgtggc ttggctggaa tccaggactg
     4081 tattttcatg gagaagaacc tgcagattct tccatcctca gctggccatg gcccacagct
     4141 ctgcatctgc atctgagctt ctcaagactc ctggagcatg aggggaatgg ggcggggcca
     4201 ctgctctgtg ctgacgggct ccgtctcgga gattcttgtc ctgttttttt tctgttgttt
     4261 ttttttggct ggtgctgggg acaagcctgt gcctgccaaa gctcccaggc caagtttggg
     4321 ggctggtgtt tggggttggg tttggggttc aggatgctgc agtctgtgca ataataaacc
     4381 cgcatctgct cacgggctct ggctgggctg caatgtgctt gtgggtccag gatgaggggc
     4441 tggctcaagg agggtgggtg ggaagaggct gggggacgtg ggtccttccc atctgtgtcc
     4501 ttgttctcac gatccttgag gggcagctga agggcttcag tttgagtggg ggctgaaaga
     4561 ccatagagct tctacagtgg agtttctaaa gcaaacttcc agaaacttct atgagtagat
     4621 gacaatagaa tcaagagcct gccctttgag gtggagtccc cccagcctcc aatgctagcc
     4681 cagccacttt cttcgcttct ctgagcctca gttttctcat ctataaaatg gggataatta
     4741 cagagcctac ctcctggtag tgaggattac attagatgtt gaggctgtag aaagcagtgg
     4801 agaaatggag acgagtgttg ttatcacgct atggtgctgt tagctctgct acaagccctc
     4861 ttgcgttttg gtagaggatc ccagggaccc caaagggatg gaggtgattc agataccagt
     4921 tgtttagtca gaatatctca atcagtgggg gagcctgagg ggatcgccac attccacaga
     4981 ggcttagagc agagggactt gcccaaggtc acgcacacag caagttggtg gcaggaaagc
     5041 agttctcttg actttttagg aataagtact gcccatgaag ttactctcac agccggtggt
     5101 tcccagcgag ggggttggag aagaaggtcc ctcaaccaac agtaggctcg agttcaggca
     5161 caccctgtcc tcaggagcct ggctccccta cccaacccag ccctatcact gatgggccat
     5221 gtgctccctc actccctctt tctgtgcctc agtttcctct tctgctgagg agactctaat
     5281 ccctgcccca tggtcttcct cataggcaat gcagttgtga ggctcaaatg agatagatta
     5341 tggtgataaa gaggttttaa gctgggcatg gtggctcaca cctataattc cagcacgtta
     5401 gaggaggctg aggcaggtgg atcacttgag gtcaggagtt tgagaccagc ctggccaaca
     5461 tggcaaaacc ccatctctac taaaaaagaa aatacaaaaa ttagccaagt gtggtggcgc
     5521 gtacctgtaa acccagctag ttgggagact gaggcaggag aagcacttga accctggagg
     5581 cggaggttgc agtgagccaa gatcaagcca ctgcactcca gcctgggtga cagagcaaga
     5641 ctccatctgg aaaaaaaaaa aaacacaaaa gttttcaaaa ctctggaatg atgctgggat
     5701 tccaggaagg atgggcttgg aggaagcaca ggaggggagg tgggagtgga aatggggagg
     5761 gaattgttat ctgtagcctt tgataaaaac ccacagaggg aaagcaggtg ggcagccagc
     5821 ccagttggcc ggtctgcaga tggggtgtgt ctgtgtgtgt gtgtgtatgt gtgtgtgttg
     5881 gatgagttgg gggcaggggt ggctgtattg aactcacttt ggatggtgtg caaaggaggc
     5941 ggcttcctga gccccaggtt tggagactca gagccaggta gggttagtcg gagacagcac
     6001 cccctgttgg ctcaagggta ggacacagcc aggccgatgc ctcctcttct aatcttgacc
     6061 acaggcagct ctaggaagtc tgatgggtct cctgccatgc ccggaatgca tttaaggtgg
     6121 tttgtaccat gcatgaactt aaaagcaatg ctggcccact gccttgaggt ctctgcagga
     6181 ctcttccagg gcagtgaaag ggaggggacc taggaaccgt gggagtcaga tttgggattc
     6241 gggcaagaag ggaagtttca taatctgatg gacccacaga agacctggct gttctgggag
     6301 agatgcaggc ccagatgatg acctttggag gtgccatgcc tgaaaaactg atatgaagat
     6361 gctctccccc aaataagtga ctcagaatac aaataatacc taaactgtaa aacataaaac
     6421 ttgcatgtat gctgaaatta aaatagctct taggttcttg ttttgtgcaa aactctgact
     6481 atgcctatgg aggccagttc ccactccctc actctctcag cctgtcttgc tggtacactc
     6541 aatggagtca ggtggccgta ggtcaggccc tgtgctaagc ccttcacctc ctgtatgtgg
     6601 tggcagaaca gctgtgtggt tcagaacatg gttgctggtt caaatcttgg ctctgtccct
     6661 taacatctag aactttctta cttttctggg ggtgatgaat accatgttac ctgcctcttc
     6721 agatagaaaa tatgaacaca gcacttagaa caattcccag catggtgtga gcagatggta
     6781 aatgcactgc tattttccat tcttcgcagt atctccatgg tgtaggtatt cgtccaccca
     6841 ttttgcagat gaggaaaatt gagccttggg gagatggagt gattggttca aggtatcaca
     6901 gagagggaag gacagagctg ggactgaaac ccagagctgt gctcttttac agaaccacac
     6961 tgctcccacc atgaaagagt gagcttccca tcactgcaga tgagccagct gaggggagat
     7021 gctagtcaac agggatgtgg caggggacat ttgtctattg gatattagtg gatgtgatga
     7081 ttcagaattc ctgaaggagt tcagggaagg aagtttcctt cttctctgaa gaagcttgag
     7141 gaaataactc cttaggttgt cagagatgga ggcactctaa gatatcagct catctggccc
     7201 cctgtttttc atatggggaa acaggtctgg agagagtgtc gtgatttgta aggatcacac
     7261 agcaagtcag tgacagagcc tggactgagc ggggagtcat attttgtcca cagaccaact
     7321 gcaggtgaga ccctcatgta cggcaacaga tgccagggca cctcatgtgt cagaacattc
     7381 accattcact ccaggctgtc ttgaccaggt gcctacatta cagtcacttc ttcttcctct
     7441 tttctttttt tttttttttg agacagagtc tcattctgtc acccaggctg gagtgcaatg
     7501 gtatacagtc acttcttagt gaccttttaa agctatttac atcttgaggc tgtaaagtca
     7561 gcaatcttga ggctgtaaag tcagcaaagt ctgagggaac aatctccccc tctcccttca
     7621 gaccccagat acaagtttgg ggatctccag ggccaccatc agttcatact gactggctac
     7681 aaacaagggg gttcctatga ttatcctcat actcaataat tcactagaaa gactcacaga
     7741 actcactgaa agctattcta ctcaagtcac attgattaca agcaaagaat gcgaatcaga
     7801 accagccaag ggaaagggac ataggaagga atctgggagg gttccaaatg ctaaacaccc
     7861 gtgtcctcgg tatctgttac cttgccagca ttgataagta acagtatgac aaagtatggc
     7921 caaccaggga agctaaccag tgcttcagtg tccagagttt ttattgggac ttcattatgt
     7981 agaggtgatt gattgaatca tttcccacat ggttgaactt aacctccata cccctcccca
     8041 cacccaaagg tcaggctgac atcacatagc ccaaagcccc aatcttttaa ccctgtggtt
     8101 gatttttctg gcatggccag cactcactat aagccatctg gttagcataa gctgtgagtt
     8161 gtggtcccag gagcccacca tgaataacaa agacactcct gttaatcagg atatcctaag
     8221 ggtttagaaa ttacttccta ggagccagga caaaaatcag acctctccct caaggcccag
     8281 ttgctaacta cacaaccctg tatctcttcc tatccacagc ttctccacta gactgagttc
     8341 cttaagagag gggctatctg tgattcatct ctatatccat gcattagtta gtgtaagctt
     8401 ggctgaggta attgactcca cagtttataa ttgctataca ccatataagt ttgtcataga
     8461 atagctgtcc ctcatcataa tgattcaggg acccaggttt tttccatctg tggttctaca
     8521 tatcctaggg cttttttggt ctctgcatct gccaaaggaa aaggaaagag agtatggagg
     8581 atggaatttg cttcctaaaa actttcacca ggaaaacagc acacatcact tctgctcata
     8641 ttccactagc cagagctcag ttgtttggcc tcaactagct tcaagggagg ctgggaaata
     8701 cggactacat gtgtcctcag ggagaagaga aggatgtggg ttttggtaaa cagacaagac
     8761 agtacagcct gtagtaccat ccccattgtc ccacttaggt ctcggggcag agtagacatt
     8821 cagtgagtgc ttattgagga aatggatatg gtggccataa gaggcagtat ggttggggag
     8881 tggggatggg ttggggcttt ggaattggac agacctagtt tctaatcctg gctatatcac
     8941 ttctttgctg tatgtggaag atgccatcac tgattggatt ctgcaggagc agacactgag
     9001 agtcacctaa gctgcaagat gtttattagg gatcaaaacc tgtgaaagga aggaggaaaa
     9061 tgcagaacag ggcagaggaa gaagtctaac ttgatgcagg cctgacaaag tccaacccag
     9121 tgggagctcc agagcaagga ttgcttgtag agcatctccc attgggcttt tatacccctc
     9181 ccttgctctg ttactgggtg atatggtttg gctgtgttct cacccaaatc tcatcttaaa
     9241 ttcccaccca cacgttgtgg gagggaccca gtgggaggta attgaatcat gggggcaagc
     9301 ctttcccgtg ccgttctcgt ggtagtgaat aagtctcatg agatcgatga ttttaaaaag
     9361 aggagttact ctgcacaagt tcctctcttt gcctgctgcc atctagtaag atgtgacttg
     9421 ctcctccttg tcttccacca tgattgtgag gcttccctag ccacttggaa ctgtgagtcc
     9481 cattaaacct ttcttttata aa
//
