LOCUS       NM_020848               9324 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens junctional cadherin 5 associated (JCAD), transcript
            variant 1, mRNA.
ACCESSION   NM_020848 XM_166132 XM_929312 XM_940811 XM_944819
VERSION     NM_020848.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Xie,L., Chen,H., Zhang,L., Ma,Y., Zhou,Y., Yang,Y.Y., Liu,C.,
            Wang,Y.L., Yan,Y.J., Ding,J., Teng,X., Yang,Q., Liu,X.P. and Wu,J.
  TITLE     JCAD deficiency attenuates activation of hepatic stellate cells and
            cholestatic fibrosis
  JOURNAL   Clin Mol Hepatol 30 (2), 206-224 (2024)
   PUBMED   38190829
  REMARK    GeneRIF: JCAD deficiency attenuates activation of hepatic stellate
            cells and cholestatic fibrosis.
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Liberale,L., Puspitasari,Y.M., Ministrini,S., Akhmedov,A.,
            Kraler,S., Bonetti,N.R., Beer,G., Vukolic,A., Bongiovanni,D.,
            Han,J., Kirmes,K., Bernlochner,I., Pelisek,J., Beer,J.H., Jin,Z.G.,
            Pedicino,D., Liuzzo,G., Stellos,K., Montecucco,F., Crea,F.,
            Luscher,T.F. and Camici,G.G.
  TITLE     JCAD promotes arterial thrombosis through PI3K/Akt modulation: a
            translational study
  JOURNAL   Eur Heart J 44 (20), 1818-1833 (2023)
   PUBMED   36469488
  REMARK    GeneRIF: JCAD promotes arterial thrombosis through PI3K/Akt
            modulation: a translational study.
            Erratum:[Eur Heart J. 2023 May 21;44(20):1843. doi:
            10.1093/eurheartj/ehad174.. PMID: 37171028]
REFERENCE   3  (bases 1 to 9324)
  AUTHORS   Douglas,G., Mehta,V., Al Haj Zen,A., Akoumianakis,I., Goel,A.,
            Rashbrook,V.S., Trelfa,L., Donovan,L., Drydale,E., Chuaiphichai,S.,
            Antoniades,C., Watkins,H., Kyriakou,T., Tzima,E. and Channon,K.M.
  TITLE     A key role for the novel coronary artery disease gene JCAD in
            atherosclerosis via shear stress mechanotransduction
  JOURNAL   Cardiovasc Res 116 (11), 1863-1874 (2020)
   PUBMED   31584065
  REMARK    GeneRIF: A key role for the novel coronary artery disease gene JCAD
            in atherosclerosis via shear stress mechanotransduction.
REFERENCE   4  (bases 1 to 9324)
  AUTHORS   Xu,S., Xu,Y., Liu,P., Zhang,S., Liu,H., Slavin,S., Kumar,S.,
            Koroleva,M., Luo,J., Wu,X., Rahman,A., Pelisek,J., Jo,H., Si,S.,
            Miller,C.L. and Jin,Z.G.
  TITLE     The novel coronary artery disease risk gene JCAD/KIAA1462 promotes
            endothelial dysfunction and atherosclerosis
  JOURNAL   Eur Heart J 40 (29), 2398-2408 (2019)
   PUBMED   31539914
  REMARK    GeneRIF: The novel coronary artery disease risk gene JCAD/KIAA1462
            promotes endothelial dysfunction and atherosclerosis.
REFERENCE   5  (bases 1 to 9324)
  AUTHORS   Jones,P.D., Kaiser,M.A., Ghaderi Najafabadi,M., Koplev,S., Zhao,Y.,
            Douglas,G., Kyriakou,T., Andrews,S., Rajmohan,R., Watkins,H.,
            Channon,K.M., Ye,S., Yang,X., Bjorkegren,J.L.M., Samani,N.J. and
            Webb,T.R.
  TITLE     JCAD, a Gene at the 10p11 Coronary Artery Disease Locus, Regulates
            Hippo Signaling in Endothelial Cells
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (8), 1711-1722 (2018)
   PUBMED   29794114
  REMARK    GeneRIF: JCAD negatively regulates Hippo signaling in endothelial
            cells and contributes to atherosclerosis by regulating YAP activity
            and endothelial dysfunction.
REFERENCE   6  (bases 1 to 9324)
  AUTHORS   Murdock,D.G., Bradford,Y., Schnetz-Boutaud,N., Mayo,P., Allen,M.J.,
            D'Aoust,L.N., Liang,X., Mitchell,S.L., Zuchner,S., Small,G.W.,
            Gilbert,J.R., Pericak-Vance,M.A. and Haines,J.L.
  TITLE     KIAA1462, a coronary artery disease associated gene, is a candidate
            gene for late onset Alzheimer disease in APOE carriers
  JOURNAL   PLoS One 8 (12), e82194 (2013)
   PUBMED   24349219
  REMARK    GeneRIF: Results in this study identify the KIAA1462 locus as a
            candidate locus for late onset Alzheimer disease in APOE4 carriers.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9324)
  AUTHORS   Akashi,M., Higashi,T., Masuda,S., Komori,T. and Furuse,M.
  TITLE     A coronary artery disease-associated gene product, JCAD/KIAA1462,
            is a novel component of endothelial cell-cell junctions
  JOURNAL   Biochem Biophys Res Commun 413 (2), 224-229 (2011)
   PUBMED   21884682
REFERENCE   8  (bases 1 to 9324)
  CONSRTM   Coronary Artery Disease (C4D) Genetics Consortium
  TITLE     A genome-wide association study in Europeans and South Asians
            identifies five new loci for coronary artery disease
  JOURNAL   Nat Genet 43 (4), 339-344 (2011)
   PUBMED   21378988
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 9324)
  AUTHORS   Erdmann,J., Willenborg,C., Nahrstaedt,J., Preuss,M., Konig,I.R.,
            Baumert,J., Linsel-Nitschke,P., Gieger,C., Tennstedt,S.,
            Belcredi,P., Aherrahrou,Z., Klopp,N., Loley,C., Stark,K.,
            Hengstenberg,C., Bruse,P., Freyer,J., Wagner,A.K., Medack,A.,
            Lieb,W., Grosshennig,A., Sager,H.B., Reinhardt,A., Schafer,A.,
            Schreiber,S., El Mokhtari,N.E., Raaz-Schrauder,D., Illig,T.,
            Garlichs,C.D., Ekici,A.B., Reis,A., Schrezenmeir,J., Rubin,D.,
            Ziegler,A., Wichmann,H.E., Doering,A., Meisinger,C., Meitinger,T.,
            Peters,A. and Schunkert,H.
  TITLE     Genome-wide association study identifies a new locus for coronary
            artery disease on chromosome 10p11.23
  JOURNAL   Eur Heart J 32 (2), 158-168 (2011)
   PUBMED   21088011
  REMARK    GeneRIF: KIAA1462 gene encodes a yet uncharacterized protein, on
            chromosome 10p11.23 with genome-wide significant association for
            CAD/MI
            GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   10 (bases 1 to 9324)
  AUTHORS   Chapuis,J., Hot,D., Hansmannel,F., Kerdraon,O., Ferreira,S.,
            Hubans,C., Maurage,C.A., Huot,L., Bensemain,F., Laumet,G.,
            Ayral,A.M., Fievet,N., Hauw,J.J., DeKosky,S.T., Lemoine,Y.,
            Iwatsubo,T., Wavrant-Devrieze,F., Dartigues,J.F., Tzourio,C.,
            Buee,L., Pasquier,F., Berr,C., Mann,D., Lendon,C., Alperovitch,A.,
            Kamboh,M.I., Amouyel,P. and Lambert,J.C.
  TITLE     Transcriptomic and genetic studies identify IL-33 as a candidate
            gene for Alzheimer's disease
  JOURNAL   Mol Psychiatry 14 (11), 1004-1016 (2009)
   PUBMED   19204726
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL158036.14 and CB988996.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020848.3.
            
            Summary: This gene encodes an endothelial cell-to-cell junction
            protein. Naturally occurring mutations in this gene are associated
            with coronary artery disease, late onset alzheimer disease, and
            emphysema distribution. [provided by RefSeq, Mar 2017].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.100409.1,
                                           SRR1803614.16211.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2467143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375377.2/ ENSP00000364526.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL158036.14        76745-76771         c
            28-445              CB988996.1         33-450
            446-4209            AL158036.14        43288-47051         c
            4210-9324           AL158036.14        29988-35102         c
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.23"
     gene            1..9324
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /note="junctional cadherin 5 associated"
                     /db_xref="GeneID:57608"
                     /db_xref="HGNC:HGNC:29283"
                     /db_xref="MIM:614398"
     exon            1..105
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /inference="alignment:Splign:2.1.0"
     exon            106..445
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..4244
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /note="isoform 1 is encoded by transcript variant 1;
                     junctional protein associated with coronary artery
                     disease; junctional cadherin 5-associated protein"
                     /codon_start=1
                     /product="junctional cadherin 5-associated protein isoform
                     1"
                     /protein_id="NP_065899.1"
                     /db_xref="CCDS:CCDS41500.1"
                     /db_xref="GeneID:57608"
                     /db_xref="HGNC:HGNC:29283"
                     /db_xref="MIM:614398"
                     /translation="MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQ
                     NGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSA
                     WSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRS
                     EHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGE
                     HVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSE
                     KSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPG
                     LEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPG
                     TGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDD
                     KSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDG
                     ENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTR
                     TKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSL
                     LSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWP
                     GHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGD
                     HKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPK
                     REVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSS
                     SSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSE
                     SWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSP
                     FPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGA
                     GLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAAR
                     ILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFY
                     GRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQK
                     DVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRM
                     KEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSV
                     SKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV"
     exon            446..4209
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..9324
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7640..7645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /note="hexamer: ATTAAA"
     polyA_site      7673
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
     regulatory      9305..9310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /note="hexamer: AATAAA"
     polyA_site      9324
                     /gene="JCAD"
                     /gene_synonym="KIAA1462"
                     /note="major polyA site"
ORIGIN      
        1 gggcatgctc ggcctcggcg gctgcaggcg gggcgggtcg cgctggtccc cggccggccg
       61 gagcggcggc ggtggcggcg cgaggcggca tgagggagcc gctcgtgctg ggtcctgcca
      121 ccaccatggt tccagtggtt gagctttgct gaagcccagg catcatgtac agtgtagaag
      181 acctcctgat ctctcatgga tacaagctgt caagagaccc cccagcatca cgcgaggata
      241 accccaaggg gcgccaggca gcgaggactg ggacacgagc aggccagggc ctgcagaacg
      301 ggcatgagga tggccctgcg gccctcgcac atcgtaagac gtccgcgggg aaaggacatg
      361 tgagtgactc cgaaagccgc cgcagcacac cgagaggcca cggggagccc cagagcactt
      421 ctgcttccag aacctcggag gcggggtttt gtaatcaacc cccctcagca tggtcctctc
      481 atcccccgac tggtaacgac caagcctacc ggagaagagg acggcaagaa gccaggagcc
      541 agaagccgag ggagcacgaa aacctggagg ccagaggaat ggcccaagcc cacagcctgc
      601 ctgtccacgt gagggagggt ccatgggaag ttggaggaag gtcagagcat gtgatgaaga
      661 agccagtttg ggaagaagaa ttgcgaatgt caggtcctgc caagtggcag aacgtcagcc
      721 tggaaagctg gaaccagcca aggaaattag ggaggcagat gtctgatgga gatggggaga
      781 gactgtttca agacctgtac ccattcattc aaggagaaca tgtgttgaat tctcaaaaca
      841 aagggaagtc tcgctcactg cctagagttc tttcccccga gagcctgagt tgcacggaaa
      901 ttcccattcc attaaatgaa agacattcac ctaaaatgcc accgtatcct cccacttgcg
      961 caccaaattt ggactccacg aggaattctg agaagagtgg ctgctcagcc ccatttcccc
     1021 ggcctaagtt tgggaggccc ctcaagcccc catcttacag ctcgcaccag cagtctaggg
     1081 gaggagcgga cagcagtgac tctcaggaca gccagcagat ggacgcctat gtccccaggc
     1141 atgagctctg cctgtcagac cctggattgg aacctccagt gtacgtgcct ccgccctcat
     1201 acagatcgcc cccgcagaac atcccaaacc cctacttgga agacacggtg cccataaatg
     1261 tgtgtggcgg tcacagtcaa cagcagtctc cgaccgagaa ggctggggcc agcggtcagc
     1321 ctccttcagg cccccctgga actgggaatg agtatggtgt gagcccccgc ttgcctcagg
     1381 ggctccccgc acatccccga cctgtcactg cctatgacgg cttcgttcag tacattccct
     1441 ttgatgatcc acggttacga cattttaaac tagctcagcc ccagggtttc tgtgaagaca
     1501 taaagcttga cgataaatca tataactcca gtcctgtcac tgctcaagag ccggctcatg
     1561 gaggaatgca gcctgatggt gccatttgga atccacagag cttaataccc ccgtcggggg
     1621 atgagagagg cctggtcttg gccgattcca gcccccggtg gctgtggggc cagccccccg
     1681 gggatgggga aaacagtggc ctccccaacc agagagaccg ctgtgtggca aggggacagt
     1741 ggcctgatgt gagaggcagc cagcacgggc acactggaag acaagtttcc tccccttact
     1801 cacagggcga gagcacctgc gaaactcaaa ccaagctcaa aaagttccaa actgggactc
     1861 ggaccaagaa aagttcaaag aaaaaaatga acgagactat attttgcttg gtttctatcc
     1921 cagtgaaatc agaatcacat ctgccagata gagatatgga caacaatgac ttaaagccca
     1981 gtgctgatca aaagaatggg tctgataaga gcccggctct gcaagaacag agtctgctga
     2041 gcatgtcttc caccgacctg gagctgcagg ccctcacagg aagcatgggt gggagaacgg
     2101 agttccaaaa acaagatcta ggggaaccag aagaagacag acaaacaaat gacctcagtt
     2161 tcatccacct tacaaagcac agagaactca agcattctgg ctcttggcca gggcaccggt
     2221 acagagatca gcaaacacaa accagtttct ccgaggagcc ccaaagttcg cagctgctcc
     2281 ctggtgcaaa gctgggaggg ccgagtcgtg cagcattgag tccaaaatgt tcagaccctg
     2341 ctgcctccga agctcagacg cacacagcat tccctaccgg tgatcacaaa cagaggccaa
     2401 gtgcccgtaa cctgaaaggt cacaggtccc tcagcccatc cagcaacagt gcgttctcaa
     2461 ggacttcctt gtccgtggac caggcaccga cgccaaaagc aggccgaagt cagccctgcg
     2521 tggatgtcca cgggcttgga gcccaccctg ggcctaagcg ggaggtggtg aagggggagc
     2581 ccacgggccc ttgcaacagt aaacaactct ttgggcagtt tctcctgaaa ccggtcagcc
     2641 gtcgtccctg ggatttgatc agtcagttag aaagttttaa caaggagctc caggaagagg
     2701 aagaaagcag cagtagcagc agcagcagca gcagcagcag tgaggagagt gaggcggagc
     2761 cgcagcagga gaaccgtgct cactgcagac aggaggatgt gggcttccgc ggaaacagcc
     2821 cggaaatgag ggttgagcca cagccgagga tgtgggtgcc ggagagccct gtgtgtaggt
     2881 cgggaagagg tgagagtaag tctgagagct ggagtgagga gctgcagcct ggccacccac
     2941 gtgcctggcc tccatccccg ggccgctttc gcgtggaaga aggtggcggt gcacctttct
     3001 gctcagcaga tggaagcacg agtgcagaga agagacacct ggaggttagc aacggaatgg
     3061 acgagctggc aggtagccca tttcctgtga cgagaatgtc ttcaagatca agtgacgcaa
     3121 aaccactgcc cgcgtcctat ccagctgaac ctagggagcc ccaggaaagt ccgaaaatca
     3181 ccagtgcttt cagctctgtg aaaccaagtg aagcggtccc tcggaagttt gacagtggtg
     3241 gagagagggg ggcagggctc ccactgtccc tgtctaacaa gaaccgaggg ctctcagctc
     3301 cagacttacg gtctgtgggg ctcacccctg ggcaagaaca gggtgccagt gagctagagg
     3361 ggtctttggg tgaagcaagc acaatagaaa tccccccagg tgagtccttg caagccaggg
     3421 ctgcaaggat cctgggcatt gaggtggcgg tggagtccct cctgccgggc atccggagag
     3481 cgggacagaa ccagcctgct gagcccgatg caagtgcctg caccccagag tccccccagg
     3541 aagagttgct atctcgccca gcaccggcag atgtccccag ggtgtccact gatgcctttt
     3601 atggcaggag gaagtgcggc tggaccaaga gccctctctt tgtaggggac agggacagtg
     3661 ccaggcgggc tcctcaggct tttgagcact cagatgtgga cggggttgtc accagcacag
     3721 accctgtccc tgagcctgag cccagcccct tggagtccaa gttcttcgaa caaaaggatg
     3781 tggaaacaaa accacccttc aggtccactt tattccattt tgtagaaaga accccaagtg
     3841 tggcaggctc tgaaaagaga cttagaagcc cttccaaagt gattgaaagt ttacaagaga
     3901 aactggcctc cccgcctagg agagcagacc ctgaccgcct gatgagaatg aaagaggtga
     3961 gctcagtgtc acggatgaga gtcctgagct tcaggaatgc cgactcccag gaggacgccg
     4021 aggaattgaa ggccaccaca aggggccagg ccgggctccc gggaggcctt gtgtctcctg
     4081 gcagtgggga ccgtgcccag agattgggcc actcactctc tgtgtccaag gacagcatct
     4141 ccagggaaga gaaggagcat ccggcagcac aaaaggagaa gagcatggat caagacttct
     4201 ggtgcccaga ttcctatgac cctagcagag tggagagggt gtgatgaaat gccggtggag
     4261 cttgcattcc gttgcgaatt gagtattctc aagtagctgg tagatttagt ctatcagccc
     4321 cacttcccca tgtagaagct ggaagctgct gagtttttag catcctgata acattcttta
     4381 ccactgccac taggaaactt gccatcctct cagaattgct ttgtggcgat aaagcaatcg
     4441 tccgttgaaa ccatctgttt ttatagcatg aagatttaat gcctataatt aggaaggagt
     4501 tttcacagaa accctccctc atgacagatt tgactaagct ggtaaatagt ttggtgttgc
     4561 tctcattctg ggtgaagatt tcaatgtgga agatctagca aatgctttgg aaaggcctgt
     4621 gaatggtgtg ctcccctctg tgtctccatg gcctgcagtc catgctcttg tgaaggagtt
     4681 tgggacactg gctgttgccc ggtgtttcag cactttcagt ttaggggaaa tgtgaatcaa
     4741 taggtatgat ccatttctaa tcaatttggc cttctgctgc tggaagtaac tttacttata
     4801 agtaacttgc catcagagaa taaaagcaat atcttttaag ttcctgaaaa attaaaactt
     4861 gtactgtaat atactaagag tattatttat ctggcagtga aatattttga tcatttgcaa
     4921 acacgccatt gtctgctttg cactcagtat tatctatttt tattttctgt atgggtattt
     4981 agaaattctt tatatgaaaa ataattgctg gtttaaaata gaccatttta acaaagtgac
     5041 tatatttctt tttacgaaaa tgctattttt ctatgatgta aacaattgtt aggcggcaga
     5101 tttttaagga agtattattt tatttaagag tctgtatccc tttgtgtgag aggaacccaa
     5161 agagggttca cttttgcttt aaagctctgt cccttaccca ctgacaactc tgctactttt
     5221 attttatctt agaaaatgtc aatatagaca tatcacaggg ttaataaaag aaacttttca
     5281 caaagatttt tgtacaagtg tagctatcag gttatatttg aaaataaccc tctgaaaacc
     5341 agccaacatt gtcatgacaa tttttgctgc cgtatcaact agaattacca aaaaacatag
     5401 ttttatcttt gtatgtgtgt ggggatctac cctgagacca tgcttttctt cccaccctgc
     5461 caccccaccc ctttaaagaa atacgtattt ttatctagaa tgatcttgaa ttagtctctg
     5521 catattttta gaaagtgctt ggggacatta tatatatgcc attacaaaag cattatgagg
     5581 cctatgtatt ctactgtgtt taatactgca ttaacctgca caaagtttat gcattttttc
     5641 atgcattttt ttccttcttt ctaagtaagg tattcctgac aactaacatt ccctaaatca
     5701 tttcccctcc ctccctccct ccctcccttc ctttcttcct tccttctgtc ctccctccct
     5761 tgctacctct tgccctcctt ccttcaccct gtcctccttt tcccttttat tttttcccct
     5821 tctctttcat tttattttct tttagagaaa ccaccttatc tatttttaga catttcgaat
     5881 tcctaaaatg tgaaccgttg ggtgatctgt cctttggccc taaaacggct ggccgggctg
     5941 tcccggcctg ttctcatctg taccgtatga ttttatttca taataacttt atgtatttgg
     6001 actgtaacaa acaaaacaga acctaagtcg ccccgaaaaa tctttccaag ccttttcaaa
     6061 acacttatct gcagtccaga attcagctct tatgaaaagt gagagtgcac tccagttttc
     6121 acagcagcaa taagtgcggg aaacaaaaag atcctcttcc caggaaaagt tgagtgggga
     6181 agagttcaga cactgttgta gctggtgaat cattgaagtc acctggtagt accaaataag
     6241 ggctgaggcg cctggaggtc tttgtggggt cttccctctt taaatgcaaa cgtaagaagg
     6301 tatatgtgta tatacattta tatacgtgca catgtatact cgtacatgta tgcacacata
     6361 cccatacatg tatatgaact ccgttctgat ttcctctgaa attttgttga taccatctaa
     6421 atacctcttt gaatcaaaat aacttaattt tagctcattc ctcaagagaa agagaaggat
     6481 ccccaaggaa gaaaaactgt tgtaccttct gtgattctca aaccaaaatg tgatgcgctt
     6541 ctgttttgtt atcttaaatt gttgtatcat atctttctga aaccattctg aattaagttg
     6601 aaattttcaa catttatact ttaactgttc taaatttaag cctacatgta aatctttttt
     6661 ggggggaagg taacttgaaa agtttaactt gaaaatttga cctttaaact ttcatattct
     6721 aaggtaaata atagcctata accaacaaca gctttcccag aggtcttgaa tttaacacta
     6781 attgtttagt tattgtttct atttctcata catacataac ttcgtttttt gtggctggaa
     6841 gaaaaagttt ctatattttg tatttgtctt tgttcttctc atgcacacaa tttactgttg
     6901 gtgtttaata atttggagct cataaaatta aaagttgtaa ttttgaccat ttgggctaaa
     6961 aattatatga gggattacat atgcaatgga ttagtatttc ttgatttgca aaagaaggga
     7021 aaatgatggt aatttagctc aaattgcctt tctttgaaga aaatttctga atgactgaaa
     7081 accaaatatc tatattatca ctttcaacct catgtttctg caaagtacag aaaaactcct
     7141 cactgacagt gcacccagcc cctgactggg ttttcaggat tatgtccatg tgcataactg
     7201 cttggatttc ttcttttaaa gtccctaaaa catgaatctg ttctttgaga tcctggtttc
     7261 cttagcagag ttgtaggttg cgggtttcat tcacgtggaa attctaagta gaacttttgc
     7321 tgccccattt tactgttggt gaagggccgt tgttaatgga agtttacgtt ttaaaactca
     7381 gatgactcag aaaccagact gtgatcattt tttgcagcag caaaatgact tattctgaca
     7441 gcatctcggt ttggggcttc tgaagaaaaa ggatgaagct gatctgttga aacaactact
     7501 ttcccaactc ggaggcaaaa ggggctgata gaagataaca ttcacctacc atttcatctt
     7561 gtgaccccca gagatcacgt ttgcgtgttc tggctgagtt ttttattatt gtttgtttta
     7621 aatgattaaa taatacacaa ttaaatgtca ggatagcatc tcttgcaaag tgaccagtgc
     7681 agcagaattg tctttctatc catatttgag cttagagaaa catttcccat gtcctaagtt
     7741 cttagaagca attactttag cgttggggag cattgttcta ccagacccat ttatgcaggg
     7801 gagatgaagc ttaaaagacg ggatgtgggg tgggagtgtg tttcttaagc cgaagctgtt
     7861 gcaggctggg ggatttttgc attttctttt tgttttgttt ttgactgcag attctgtaca
     7921 tctgtctgtg ggaggagagt tgctactcag gacaggattt aagagacaca ttccagtgac
     7981 ctttaagaat ctgcatggcg ggaggtcctt ctccaggagt gtgggttggt ccactctggg
     8041 acccaccaca ctaagaaggg ggagatgata aaataacatt aaaggaagaa tggcctccag
     8101 cctgcaggtt ttgttggaaa gaaataaaaa gggagtcatt aagaccataa attcagattg
     8161 aggcctcttg aaaaggttga tgtgggctag caacctgcct gtcgaaacag tccttggttc
     8221 atcagcagtg tgggaaggca gccaaggctc ctgcagattc ctggcatcga ccttggaaag
     8281 cctctgcgat acttgtgtgt acgaatacag cagaggacag ggaggtcctt gttctgtggt
     8341 ctctgtttag tgactgaaac ttaaacccaa aggcaagccc agattgtctg gccgttcatc
     8401 cccatgcttt gataggggtt aggaggaacc ctttccgtat gaaagacagg ccctactagg
     8461 gcttacaacc aagccaaagg accatctctt ctttcttcca cctcccttca cccctgcccc
     8521 gcagcagagc cgagatgtga gacattcatt gtcacggagc aaggagacaa aggcaagttc
     8581 aagttgagaa gcatatggca gcaaacagaa atgaaaacca tatgtcccag caaggggaaa
     8641 agcagtcatt tccagattat aaaaatcaat gaagtactct ccacctaggt cagctgaaat
     8701 tcgagccctc acagtcaggc ctgtcagaga agttaagcag aaacatctcg gggggacttc
     8761 taaaatttag tgaagacaag gccttgcaac tccaaagaaa ctttttttcc cccccttgaa
     8821 acagggtctt gctctgttgc ccagcctgga gtgcagtggt gcagtcacgg ctcactgcag
     8881 cctcaagctc ctgggctcaa gcactatccc cacctcaacc tccttagtag ctgggactac
     8941 aggtgcacac caccatgccc agctaaccac agaaactttc atctcttcat tttttctttg
     9001 ggcaccatta atacctaaga caggtagaaa gggtcccaga aagacaccat tggtaatggc
     9061 cgattgccgg ctgcagtcat cgcccccaga tcaggctggt acaggatgcc ttaaggtgat
     9121 gagaggtgag ggtgcatgaa gaataatgag cacagggaag agagaagcag gacaaagtag
     9181 cagataaaat gccggcaaag cacagatgaa tgtcttcaag aagctcttgt atttctctgc
     9241 acagtgtaaa tatccttgct atttcaggat ggcggctggc ctgctcagta acatacatgt
     9301 tccaaataaa gattttgcat gaaa
//
