LOCUS       NM_004972               7023 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens Janus kinase 2 (JAK2), transcript variant 1, mRNA.
ACCESSION   NM_004972
VERSION     NM_004972.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Shin,J.J., Suk,K. and Lee,W.H.
  TITLE     LncRNA BRE-AS1 regulates the JAK2/STAT3-mediated inflammatory
            activation via the miR-30b-5p/SOC3 axis in THP-1 cells
  JOURNAL   Sci Rep 14 (1), 25726 (2024)
   PUBMED   39468152
  REMARK    GeneRIF: LncRNA BRE-AS1 regulates the JAK2/STAT3-mediated
            inflammatory activation via the miR-30b-5p/SOC3 axis in THP-1
            cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Chen,H., Ge,X., Li,C., Zeng,J. and Wang,X.
  TITLE     Structure and assembly of the human IL-12 signaling complex
  JOURNAL   Structure 32 (10), 1640-1651 (2024)
   PUBMED   39111304
REFERENCE   3  (bases 1 to 7023)
  AUTHORS   Peng,Z., Fang,C., Yuan,H., Zhu,Y., Ren,Z., Lu,M. and Hu,K.
  TITLE     miR-135b-5p promotes gastric carcinogenesis by targeting
            CLIP4-mediated JAK2/STAT3 signal pathway
  JOURNAL   Cell Signal 122, 111339 (2024)
   PUBMED   39121973
  REMARK    GeneRIF: miR-135b-5p promotes gastric carcinogenesis by targeting
            CLIP4-mediated JAK2/STAT3 signal pathway.
REFERENCE   4  (bases 1 to 7023)
  AUTHORS   Haage,T.R., Charakopoulos,E., Bhuria,V., Baldauf,C.K., Korthals,M.,
            Handschuh,J., Muller,P., Li,J., Harit,K., Nishanth,G., Frey,S.,
            Bottcher,M., Fischer,K.D., Dudeck,J., Dudeck,A., Lipka,D.B.,
            Schraven,B., Green,A.R., Muller,A.J., Mougiakakos,D. and Fischer,T.
  TITLE     Neutrophil-specific expression of JAK2-V617F or CALRmut induces
            distinct inflammatory profiles in myeloproliferative neoplasia
  JOURNAL   J Hematol Oncol 17 (1), 43 (2024)
   PUBMED   38853260
  REMARK    GeneRIF: Neutrophil-specific expression of JAK2-V617F or CALRmut
            induces distinct inflammatory profiles in myeloproliferative
            neoplasia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7023)
  AUTHORS   Zhao,Y., Liang,B., Sheng,S., Wang,C., Jin,B., Zhang,X., Cheng,Y.,
            Shen,C. and Zheng,F.
  TITLE     AIM2 inflammasome regulated by the IFN-gamma/JAK2/STAT1 pathway
            promotes activation and pyroptosis of monocytes in Coronary Artery
            Disease
  JOURNAL   Immun Inflamm Dis 12 (6), e1317 (2024)
   PUBMED   38869352
  REMARK    GeneRIF: AIM2 inflammasome regulated by the IFN-gamma/JAK2/STAT1
            pathway promotes activation and pyroptosis of monocytes in Coronary
            Artery Disease.
REFERENCE   6  (bases 1 to 7023)
  AUTHORS   Szente,B.E., Subramaniam,P.S. and Johnson,H.M.
  TITLE     Identification of IFN-gamma receptor binding sites for JAK2 and
            enhancement of binding by IFN-gamma and its C-terminal peptide
            IFN-gamma(95-133)
  JOURNAL   J Immunol 155 (12), 5617-5622 (1995)
   PUBMED   7499845
REFERENCE   7  (bases 1 to 7023)
  AUTHORS   Chauhan,D., Kharbanda,S.M., Ogata,A., Urashima,M., Frank,D.,
            Malik,N., Kufe,D.W. and Anderson,K.C.
  TITLE     Oncostatin M induces association of Grb2 with Janus kinase JAK2 in
            multiple myeloma cells
  JOURNAL   J Exp Med 182 (6), 1801-1806 (1995)
   PUBMED   7500025
REFERENCE   8  (bases 1 to 7023)
  AUTHORS   Pritchard,M.A., Baker,E., Callen,D.F., Sutherland,G.R. and
            Wilks,A.F.
  TITLE     Two members of the JAK family of protein tyrosine kinases map to
            chromosomes 1p31.3 and 9p24
  JOURNAL   Mamm Genome 3 (1), 36-38 (1992)
   PUBMED   1581631
REFERENCE   9  (bases 1 to 7023)
  AUTHORS   Wilks,A.F., Harpur,A.G., Kurban,R.R., Ralph,S.J., Zurcher,G. and
            Ziemiecki,A.
  TITLE     Two novel protein-tyrosine kinases, each with a second
            phosphotransferase-related catalytic domain, define a new class of
            protein kinase
  JOURNAL   Mol Cell Biol 11 (4), 2057-2065 (1991)
   PUBMED   1848670
REFERENCE   10 (bases 1 to 7023)
  AUTHORS   Hayashida,K., Kitamura,T., Gorman,D.M., Arai,K., Yokota,T. and
            Miyajima,A.
  TITLE     Molecular cloning of a second subunit of the receptor for human
            granulocyte-macrophage colony-stimulating factor (GM-CSF):
            reconstitution of a high-affinity GM-CSF receptor
  JOURNAL   Proc Natl Acad Sci U S A 87 (24), 9655-9659 (1990)
   PUBMED   1702217
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF005216.1, AL158147.17,
            AF058925.2, AI923337.1 and AL161450.14.
            
            On Dec 4, 2019 this sequence version replaced NM_004972.3.
            
            Summary: This gene encodes a non-receptor tyrosine kinase that
            plays a central role in cytokine and growth factor signalling. The
            primary isoform of this protein has an N-terminal FERM domain that
            is required for erythropoietin receptor association, an SH2 domain
            that binds STAT transcription factors, a pseudokinase domain and a
            C-terminal tyrosine kinase domain. Cytokine binding induces
            autophosphorylation and activation of this kinase. This kinase then
            recruits and phosphorylates signal transducer and activator of
            transcription (STAT) proteins. Growth factors like TGF-beta 1 also
            induce phosphorylation and activation of this kinase and
            translocation of downstream STAT proteins to the nucleus where they
            influence gene transcription. Mutations in this gene are associated
            with numerous inflammatory diseases and malignancies. This gene is
            a downstream target of the pleiotropic cytokine IL6 that is
            produced by B cells, T cells, dendritic cells and macrophages to
            produce an immune response or inflammation. Disregulation of the
            IL6/JAK2/STAT3 signalling pathways produces increased cellular
            proliferation and myeloproliferative neoplasms of hematopoietic
            stem cells. A nonsynonymous mutation in the pseudokinase domain of
            this gene disrupts the domains inhibitory effect and results in
            constitutive tyrosine phosphorylation activity and hypersensitivity
            to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling
            pathway is a therapeutic target for the treatment of excessive
            inflammatory responses to viral infections. Alternative splicing
            results in multiple transcript variants encoding distinct isoforms.
            [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (1), along with variants 2, 3, and
            4, all encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF058925.2, SRR18074968.3599583.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in cytokine storm inflammatory
                                      response
            MANE Ensembl match     :: ENST00000381652.4/ ENSP00000371067.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-266               AF005216.1         28-293
            267-274             AL158147.17        155883-155890
            275-2956            AF005216.1         302-2983
            2957-5070           AF058925.2         2967-5080
            5071-5258           AI923337.1         1-188               c
            5259-7023           AL161450.14        109475-111239
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p24.1"
     gene            1..7023
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="Janus kinase 2"
                     /db_xref="GeneID:3717"
                     /db_xref="HGNC:HGNC:6192"
                     /db_xref="MIM:147796"
     exon            1..359
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            360..442
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            443..693
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     CDS             468..3866
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /EC_number="2.7.10.2"
                     /note="isoform a is encoded by transcript variant 1;
                     tyrosine-protein kinase JAK2; Janus kinase 2 (a protein
                     tyrosine kinase); JAK-2"
                     /codon_start=1
                     /product="tyrosine-protein kinase JAK2 isoform a"
                     /protein_id="NP_004963.1"
                     /db_xref="CCDS:CCDS6457.1"
                     /db_xref="GeneID:3717"
                     /db_xref="HGNC:HGNC:6192"
                     /db_xref="MIM:147796"
                     /translation="MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLY
                     HSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVF
                     HIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHD
                     FVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKI
                     QDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPG
                     SGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQE
                     GSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAV
                     LENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYK
                     HCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSN
                     LLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRRE
                     VGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENIL
                     VQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLI
                     REEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFG
                     TTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSF
                     RAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLG
                     KGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYK
                     GVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRY
                     IHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESK
                     FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRL
                     PRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG"
     exon            694..817
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            818..935
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1081
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1403
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1523
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1681
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1793
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1980
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2108
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2243
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2331
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2459
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2598
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2750
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2901
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..3038
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3228
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3229..3353
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3354..3526
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3644
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3645..3758
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..7023
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5231..5236
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="hexamer: ATTAAA"
     polyA_site      5258
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="major polyA site"
     regulatory      6500..6505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="hexamer: AATGAA"
     polyA_site      6520
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="major polyA site"
     regulatory      6716..6721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="hexamer: ATTAAA"
     polyA_site      6737
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="major polyA site"
     regulatory      6998..7003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="hexamer: AATAAA"
     polyA_site      7023
                     /gene="JAK2"
                     /gene_synonym="JTK10"
                     /note="major polyA site"
ORIGIN      
        1 agaagggggc agcagcggac gccgctaacg gcctccctcg gcgctgacag gctgggccgg
       61 cgcccggctc gcttgggtgt tcgcgtcgcc acttcggctt ctcggccggt cgggcccctc
      121 ggcccgggct tgcggcgcgc gtcggggctg agggctgctg cggcgcaggg agaggcctgg
      181 tcctcgctgc cgagggatgt gagtgggagc tgagcccaca ctggagggcc cccgagggcc
      241 cagcctggag gtcgttcaga gccgtgcccg tcccggggct tcgcagacct tgacccgccg
      301 ggtaggagcc gcccctgcgg gctcgagggc gcgctctggt cgcccgatct gtgtagccgg
      361 tttcagaagc aggcaacagg aacaagatgt gaactgtttc tcttctgcag aaaaagaggc
      421 tcttcctcct cctcccgcga cggcaaatgt tctgaaaaag actctgcatg ggaatggcct
      481 gccttacgat gacagaaatg gagggaacat ccacctcttc tatatatcag aatggtgata
      541 tttctggaaa tgccaattct atgaagcaaa tagatccagt tcttcaggtg tatctttacc
      601 attcccttgg gaaatctgag gcagattatc tgacctttcc atctggggag tatgttgcag
      661 aagaaatctg tattgctgct tctaaagctt gtggtatcac acctgtgtat cataatatgt
      721 ttgctttaat gagtgaaaca gaaaggatct ggtatccacc caaccatgtc ttccatatag
      781 atgagtcaac caggcataat gtactctaca gaataagatt ttactttcct cgttggtatt
      841 gcagtggcag caacagagcc tatcggcatg gaatatctcg aggtgctgaa gctcctcttc
      901 ttgatgactt tgtcatgtct tacctctttg ctcagtggcg gcatgatttt gtgcacggat
      961 ggataaaagt acctgtgact catgaaacac aggaagaatg tcttgggatg gcagtgttag
     1021 atatgatgag aatagccaaa gaaaacgatc aaaccccact ggccatctat aactctatca
     1081 gctacaagac attcttacca aaatgtattc gagcaaagat ccaagactat catattttga
     1141 caaggaagcg aataaggtac agatttcgca gatttattca gcaattcagc caatgcaaag
     1201 ccactgccag aaacttgaaa cttaagtatc ttataaatct ggaaactctg cagtctgcct
     1261 tctacacaga gaaatttgaa gtaaaagaac ctggaagtgg tccttcaggt gaggagattt
     1321 ttgcaaccat tataataact ggaaacggtg gaattcagtg gtcaagaggg aaacataaag
     1381 aaagtgagac actgacagaa caggatttac agttatattg cgattttcct aatattattg
     1441 atgtcagtat taagcaagca aaccaagagg gttcaaatga aagccgagtt gtaactatcc
     1501 ataagcaaga tggtaaaaat ctggaaattg aacttagctc attaagggaa gctttgtctt
     1561 tcgtgtcatt aattgatgga tattatagat taactgcaga tgcacatcat tacctctgta
     1621 aagaagtagc acctccagcc gtgcttgaaa atatacaaag caactgtcat ggcccaattt
     1681 cgatggattt tgccattagt aaactgaaga aagcaggtaa tcagactgga ctgtatgtac
     1741 ttcgatgcag tcctaaggac tttaataaat attttttgac ttttgctgtc gagcgagaaa
     1801 atgtcattga atataaacac tgtttgatta caaaaaatga gaatgaagag tacaacctca
     1861 gtgggacaaa gaagaacttc agcagtctta aagatctttt gaattgttac cagatggaaa
     1921 ctgttcgctc agacaatata attttccagt ttactaaatg ctgtccccca aagccaaaag
     1981 ataaatcaaa ccttctagtc ttcagaacga atggtgtttc tgatgtacca acctcaccaa
     2041 cattacagag gcctactcat atgaaccaaa tggtgtttca caaaatcaga aatgaagatt
     2101 tgatatttaa tgaaagcctt ggccaaggca cttttacaaa gatttttaaa ggcgtacgaa
     2161 gagaagtagg agactacggt caactgcatg aaacagaagt tcttttaaaa gttctggata
     2221 aagcacacag aaactattca gagtctttct ttgaagcagc aagtatgatg agcaagcttt
     2281 ctcacaagca tttggtttta aattatggag tatgtgtctg tggagacgag aatattctgg
     2341 ttcaggagtt tgtaaaattt ggatcactag atacatatct gaaaaagaat aaaaattgta
     2401 taaatatatt atggaaactt gaagttgcta aacagttggc atgggccatg cattttctag
     2461 aagaaaacac ccttattcat gggaatgtat gtgccaaaaa tattctgctt atcagagaag
     2521 aagacaggaa gacaggaaat cctcctttca tcaaacttag tgatcctggc attagtatta
     2581 cagttttgcc aaaggacatt cttcaggaga gaataccatg ggtaccacct gaatgcattg
     2641 aaaatcctaa aaatttaaat ttggcaacag acaaatggag ttttggtacc actttgtggg
     2701 aaatctgcag tggaggagat aaacctctaa gtgctctgga ttctcaaaga aagctacaat
     2761 tttatgaaga taggcatcag cttcctgcac caaagtgggc agaattagca aaccttataa
     2821 ataattgtat ggattatgaa ccagatttca ggccttcttt cagagccatc atacgagatc
     2881 ttaacagttt gtttactcca gattatgaac tattaacaga aaatgacatg ttaccaaata
     2941 tgaggatagg tgccctgggg ttttctggtg cctttgaaga ccgggatcct acacagtttg
     3001 aagagagaca tttgaaattt ctacagcaac ttggcaaggg taattttggg agtgtggaga
     3061 tgtgccggta tgaccctcta caggacaaca ctggggaggt ggtcgctgta aaaaagcttc
     3121 agcatagtac tgaagagcac ctaagagact ttgaaaggga aattgaaatc ctgaaatccc
     3181 tacagcatga caacattgta aagtacaagg gagtgtgcta cagtgctggt cggcgtaatc
     3241 taaaattaat tatggaatat ttaccatatg gaagtttacg agactatctt caaaaacata
     3301 aagaacggat agatcacata aaacttctgc agtacacatc tcagatatgc aagggtatgg
     3361 agtatcttgg tacaaaaagg tatatccaca gggatctggc aacgagaaat atattggtgg
     3421 agaacgagaa cagagttaaa attggagatt ttgggttaac caaagtcttg ccacaagaca
     3481 aagaatacta taaagtaaaa gaacctggtg aaagtcccat attctggtat gctccagaat
     3541 cactgacaga gagcaagttt tctgtggcct cagatgtttg gagctttgga gtggttctgt
     3601 atgaactttt cacatacatt gagaagagta aaagtccacc agcggaattt atgcgtatga
     3661 ttggcaatga caaacaagga cagatgatcg tgttccattt gatagaactt ttgaagaata
     3721 atggaagatt accaagacca gatggatgcc cagatgagat ctatatgatc atgacagaat
     3781 gctggaacaa taatgtaaat caacgcccct cctttaggga tctagctctt cgagtggatc
     3841 aaataaggga taacatggct ggatgaaaga aatgaccttc attctgagac caaagtagat
     3901 ttacagaaca aagttttata tttcacattg ctgtggacta ttattacata tatcattatt
     3961 atataaatca tgatgctagc cagcaaagat gtgaaaatat ctgctcaaaa ctttcaaagt
     4021 ttagtaagtt tttcttcatg aggccaccag taaaagacat taatgagaat tccttagcaa
     4081 ggattttgta agaagtttct taaacattgt cagttaacat cactcttgtc tggcaaaaga
     4141 aaaaaaatag actttttcaa ctcagctttt tgagacctga aaaaattatt atgtaaattt
     4201 tgcaatgtta aagatgcaca gaatatgtat gtatagtttt taccacagtg gatgtataat
     4261 accttggcat cttgtgtgat gttttacaca catgagggct ggtgttcatt aatactgttt
     4321 tctaattttt ccatagttaa tctataatta attacttcac tatacaaaca aattaagatg
     4381 ttcagataat tgaataagta cctttgtgtc cttgttcatt tatatcgctg gccagcatta
     4441 taagcaggtg tatactttta gcttgtagtt ccatgtactg taaatatttt tcacataaag
     4501 ggaacaaatg tctagtttta tttgtatagg aaatttccct gaccctaaat aatacatttt
     4561 gaaatgaaac aagcttacaa agatataatc tattttatta tggtttccct tgtatctatt
     4621 tgtggtgaat gtgtttttta aatggaacta tctccaaatt tttctaagac tactatgaac
     4681 agttttcttt taaaattttg agattaagaa tgccaggaat attgtcatcc tttgagctgc
     4741 tgactgccaa taacattctt cgatctctgg gatttatgct catgaactaa atttaagctt
     4801 aagccataaa atagattaga ttgtttttta aaaatggata gctcattaag aagtgcagca
     4861 ggttaagaat tttttcctaa agactgtata tttgaggggt ttcagaattt tgcattgcag
     4921 tcatagaaga gatttatttc ctttttagag gggaaatgag gtaaataagt aaaaaagtat
     4981 gcttgttaat tttattcaag aatgccagta gaaaattcat aacgtgtatc tttaagaaaa
     5041 atgagcatac atcttaaatc ttttcaatta agtataaggg gttgttcgtt gttgtcattt
     5101 gttatagtgc tactccactt tagacaccat agctaaaata aaatatggtg ggttttgtgt
     5161 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgttatt tatacaaaac ttaaaatact
     5221 tgctgttttg attaaaaaga aaatagtttc ttactttatt tttactggta tgttctactt
     5281 ttttgaaagt tgtactgaag acttctgatt ttgggttgaa gggaaggaaa aggaagaaat
     5341 gttttttaca ttcattatta tacttaaagc atttttaaag cattttaata gttctggatg
     5401 cagaaatcat ctaaaatgac agtgaattag gttttaaaaa gattttagat ttttttgaaa
     5461 gtttaatttt tatttgtaaa gactcctcaa ggatttgtat atgcaacaca gtaaggagat
     5521 cttccatttt actacctttc aagtgaaaaa tagcctatca tacaatatgc ttgatttcag
     5581 attttcatac taaaacttaa ctacatactt aaaagtaggt tcttatcaag ggtctctaac
     5641 attgcttttt aaaacaagat gtgaactaac ttttcttaaa cattttttta aatgcttcat
     5701 cttttagttt tatataaaga atcccacatg tacattcttg tttttagaat ggggtgacta
     5761 ccttattata aaattccaag tttccaagag acttcttttc attgaggctt cgtaaagttt
     5821 tccattttga ttctgactac acataaaaat aagataaccc tgtagttatt aagttggttc
     5881 tgtacaagaa acaggtaagt aattattgta ccagttaatg ccaaaatatt tttcacgtta
     5941 atattcttca gaaacaaggg taaaggtatt cttagaatta tgtaattctg taactattcc
     6001 agtatattaa gttatacaat ctttatataa atgacttttt ccatgggtac ttgtttggaa
     6061 aatagtcact ttttcacgct atttatatat gctgccagta acactataat ttgctatgga
     6121 agagtgttct tttaacctaa ggcttctagt ttacagtcaa gtgtaatttt catcacaact
     6181 ataacttctg gtatttaaat tttatttaaa cagctacaaa agagttagca aatacctaca
     6241 gttctgtatc tatagagcca atcttgatgg tgggtgtggc attatgtgct cactttattg
     6301 agcctatgtt aatttcttta gcatgctccc cctaaattga aatagtgatg tagtaaatat
     6361 tcagaagcga ttttcttttg catttttacc taaccaagga aacgggccac acaccttggt
     6421 ttagggatgt tgtgatagct taccttccag tttttaagaa atgcttccta caactgctgt
     6481 caaccactgt attgtcttta atgaacactg ttgtatccca tcctaattct tgtactgaaa
     6541 ttatttctca tgaaagtttc tctaatattt ctaatgaaag tttctctaat ttgggggcat
     6601 aatgtactaa gaatcagttt gctgtatatt agaataaata gtaacagtaa gtcagcagga
     6661 ttatccaaac aaaagactag gttttatgag ataagcttga tttaagaaaa aaacaattaa
     6721 agtatgaata tcagaaatac tgtgtgttta ctctcagatt ttagttggtt ggatttaata
     6781 tcaagataac tagctgctaa gcgtttcata attctcacag tgatattaga tttcaaaatg
     6841 acactgagag aactgaaaaa ctacatcagt caaattcatg tatgtatatc atatagcctt
     6901 taacttttta cattaatcag attcttagta aaatgcagac tgtataccta aatattaaaa
     6961 tatttacttt tataatctta ccttttattt caatataaat aaaattcttc ttaggttaaa
     7021 aaa
//
