LOCUS       NM_001321855            5176 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens Janus kinase 1 (JAK1), transcript variant 6, mRNA.
ACCESSION   NM_001321855
VERSION     NM_001321855.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Horesh,M.E., Martin-Fernandez,M., Gruber,C., Buta,S., Le Voyer,T.,
            Puzenat,E., Lesmana,H., Wu,Y., Richardson,A., Stein,D., Hodeib,S.,
            Youssef,M., Kurowski,J.A., Feuille,E., Pedroza,L.A., Fuleihan,R.L.,
            Haseley,A., Hovnanian,A., Quartier,P., Rosain,J., Davis,G.,
            Mullan,D., Stewart,O., Patel,R., Lee,A.E., Rubinstein,R., Ewald,L.,
            Maheshwari,N., Rahming,V., Chinn,I.K., Lupski,J.R., Orange,J.S.,
            Sancho-Shimizu,V., Casanova,J.L., Abul-Husn,N.S., Itan,Y.,
            Milner,J.D., Bustamante,J. and Bogunovic,D.
  TITLE     Individuals with JAK1 variants are affected by syndromic features
            encompassing autoimmunity, atopy, colitis, and dermatitis
  JOURNAL   J Exp Med 221 (6) (2024)
   PUBMED   38563820
  REMARK    GeneRIF: Individuals with JAK1 variants are affected by syndromic
            features encompassing autoimmunity, atopy, colitis, and dermatitis.
            Erratum:[J Exp Med. 2024 Jun 3;221(6):e2023238704302024c. doi:
            10.1084/jem.2023238704302024c.. PMID: 38709237]
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Shrestha,H., Radler,P.D., Dennaoui,R., Wicker,M.N., Rajbhandari,N.,
            Sun,Y., Peck,A.R., Vistisen,K., Triplett,A.A., Beydoun,R.,
            Sterneck,E., Saur,D., Rui,H. and Wagner,K.U.
  TITLE     The Janus kinase 1 is critical for pancreatic cancer initiation and
            progression
  JOURNAL   Cell Rep 43 (5), 114202 (2024)
   PUBMED   38733583
  REMARK    GeneRIF: The Janus kinase 1 is critical for pancreatic cancer
            initiation and progression.
REFERENCE   3  (bases 1 to 5176)
  AUTHORS   Yu,Z., Zhang,Q., Wei,S., Zhang,Y., Zhou,T., Zhang,Q., Shi,R.,
            Zinovkin,D., Pranjol,Z.I., Zhang,J. and Wang,H.
  TITLE     CD146+CAFs promote progression of endometrial cancer by inducing
            angiogenesis and vasculogenic mimicry via IL-10/JAK1/STAT3 pathway
  JOURNAL   Cell Commun Signal 22 (1), 170 (2024)
   PUBMED   38459564
  REMARK    GeneRIF: CD146[+]CAFs promote progression of endometrial cancer by
            inducing angiogenesis and vasculogenic mimicry via IL-10/JAK1/STAT3
            pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5176)
  AUTHORS   Cheng,B., Li,L., Luo,T., Wang,Q., Luo,Y., Bai,S., Li,K., Lai,Y. and
            Huang,H.
  TITLE     Single-cell deconvolution algorithms analysis unveils autocrine
            IL11-mediated resistance to docetaxel in prostate cancer via
            activation of the JAK1/STAT4 pathway
  JOURNAL   J Exp Clin Cancer Res 43 (1), 67 (2024)
   PUBMED   38429845
  REMARK    GeneRIF: Single-cell deconvolution algorithms analysis unveils
            autocrine IL11-mediated resistance to docetaxel in prostate cancer
            via activation of the JAK1/STAT4 pathway.
            Erratum:[J Exp Clin Cancer Res. 2024 Dec 28;43(1):333. doi:
            10.1186/s13046-024-03257-8.. PMID: 39731134]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5176)
  AUTHORS   Huang,Q., Xiao,Y., Lan,T., Lu,Y., Huang,L. and Zheng,D.
  TITLE     WNT7A promotes tumorigenesis of head and neck squamous cell
            carcinoma via activating FZD7/JAK1/STAT3 signaling
  JOURNAL   Int J Oral Sci 16 (1), 7 (2024)
   PUBMED   38246919
  REMARK    GeneRIF: WNT7A promotes tumorigenesis of head and neck squamous
            cell carcinoma via activating FZD7/JAK1/STAT3 signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5176)
  AUTHORS   Johnston,J.A., Wang,L.M., Hanson,E.P., Sun,X.J., White,M.F.,
            Oakes,S.A., Pierce,J.H. and O'Shea,J.J.
  TITLE     Interleukins 2, 4, 7, and 15 stimulate tyrosine phosphorylation of
            insulin receptor substrates 1 and 2 in T cells. Potential role of
            JAK kinases
  JOURNAL   J Biol Chem 270 (48), 28527-28530 (1995)
   PUBMED   7499365
REFERENCE   7  (bases 1 to 5176)
  AUTHORS   Velazquez,L., Fellous,M., Stark,G.R. and Pellegrini,S.
  TITLE     A protein tyrosine kinase in the interferon alpha/beta signaling
            pathway
  JOURNAL   Cell 70 (2), 313-322 (1992)
   PUBMED   1386289
REFERENCE   8  (bases 1 to 5176)
  AUTHORS   Howard,O.M., Dean,M., Young,H., Ramsburg,M., Turpin,J.A.,
            Michiel,D.F., Kelvin,D.J., Lee,L. and Farrar,W.L.
  TITLE     Characterization of a class 3 tyrosine kinase
  JOURNAL   Oncogene 7 (5), 895-900 (1992)
   PUBMED   1373877
REFERENCE   9  (bases 1 to 5176)
  AUTHORS   Pritchard,M.A., Baker,E., Callen,D.F., Sutherland,G.R. and
            Wilks,A.F.
  TITLE     Two members of the JAK family of protein tyrosine kinases map to
            chromosomes 1p31.3 and 9p24
  JOURNAL   Mamm Genome 3 (1), 36-38 (1992)
   PUBMED   1581631
REFERENCE   10 (bases 1 to 5176)
  AUTHORS   Wilks,A.F., Harpur,A.G., Kurban,R.R., Ralph,S.J., Zurcher,G. and
            Ziemiecki,A.
  TITLE     Two novel protein-tyrosine kinases, each with a second
            phosphotransferase-related catalytic domain, define a new class of
            protein kinase
  JOURNAL   Mol Cell Biol 11 (4), 2057-2065 (1991)
   PUBMED   1848670
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL606517.14 and AC093427.2.
            
            On May 31, 2019 this sequence version replaced NM_001321855.1.
            
            Summary: This gene encodes a membrane protein that is a member of a
            class of protein-tyrosine kinases (PTK) characterized by the
            presence of a second phosphotransferase-related domain immediately
            N-terminal to the PTK domain. The encoded kinase phosphorylates
            STAT proteins (signal transducers and activators of transcription)
            and plays a key role in interferon-alpha/beta, interferon-gamma,
            and cytokine signal transduction. This gene plays a crucial role in
            effecting the expression of genes that mediate inflammation,
            epithelial remodeling, and metastatic cancer progression. This gene
            is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune
            and inflammation response and is a therapeutic target for
            alleviating cytokine storms. The kinase activity of this gene is
            directly inhibited by the suppressor of cytokine signalling 1
            (SOCS1) protein. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2020].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2637827.1,
                                           SRR14038191.1645224.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related :: relevant for COVID-19 treatment
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL606517.14        51381-51597         c
            218-301             AL606517.14        41982-42065         c
            302-384             AC093427.2         9395-9477
            385-583             AC093427.2         12261-12459
            584-707             AC093427.2         16588-16711
            708-861             AC093427.2         22213-22366
            862-1025            AC093427.2         26262-26425
            1026-1368           AC093427.2         28528-28870
            1369-1554           AC093427.2         30764-30949
            1555-1712           AC093427.2         35474-35631
            1713-1836           AC093427.2         37957-38080
            1837-2026           AC093427.2         40038-40227
            2027-2133           AC093427.2         44826-44932
            2134-2277           AC093427.2         48061-48204
            2278-2365           AC093427.2         49000-49087
            2366-2493           AC093427.2         50096-50223
            2494-2629           AC093427.2         50847-50982
            2630-2781           AC093427.2         51521-51672
            2782-2932           AC093427.2         54135-54285
            2933-3027           AC093427.2         54397-54491
            3028-3220           AC093427.2         55941-56133
            3221-3345           AC093427.2         57147-57271
            3346-3518           AC093427.2         57632-57804
            3519-3636           AC093427.2         59521-59638
            3637-3747           AC093427.2         60230-60340
            3748-5176           AC093427.2         61079-62507
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..5176
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /note="Janus kinase 1"
                     /db_xref="GeneID:3716"
                     /db_xref="HGNC:HGNC:6190"
                     /db_xref="MIM:147795"
     exon            1..217
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            218..301
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            302..384
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..3843
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 is encoded by transcript variant 6;
                     tyrosine-protein kinase JAK1"
                     /codon_start=1
                     /product="tyrosine-protein kinase JAK1 isoform 1"
                     /protein_id="NP_001308784.1"
                     /db_xref="CCDS:CCDS41346.1"
                     /db_xref="GeneID:3716"
                     /db_xref="HGNC:HGNC:6190"
                     /db_xref="MIM:147795"
                     /translation="MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSD
                     REPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMS
                     LRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFA
                     QGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYK
                     RYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLET
                     LTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSV
                     EKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKM
                     ELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAI
                     NKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYS
                     LHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQP
                     VYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVL
                     DPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRK
                     SDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDP
                     GIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKT
                     LIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIV
                     SEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPES
                     GGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNK
                     NKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIET
                     DKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALF
                     LKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLI
                     EGFEALLK"
     exon            385..583
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            584..707
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            708..861
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1025
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1368
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1554
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1712
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1836
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..2026
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2133
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2277
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2365
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2493
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2629
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2781
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2932
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2933..3027
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3220
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3345
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3518
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3519..3636
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3637..3747
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            3748..5176
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5155..5160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /note="hexamer: AATAAA"
     polyA_site      5176
                     /gene="JAK1"
                     /gene_synonym="AIIDE; JAK1A; JAK1B; JTK3"
                     /note="major polyA site"
ORIGIN      
        1 gcgtcgctga gcgcaggccg cggcggccgc ggagtatcct ggagctgcag acagtgcggg
       61 cctgcgccca gtcccggctg tcctcgccgc gacccctcct cagccctggg cgcgcgcacg
      121 ctggggcccc gcggggctgg ccgcctagcg agcctgccgg tcgaccccag ccagcgcagc
      181 gacggggcgc tgcctggccc aggcgcacac ggaagtgtta tctaaaacag ttcatgctgc
      241 tgaaaacctc cttcctggca gatgtccctc aaccctactg gtgcctggct tctgagacac
      301 acgcttctct gaagtagctt tggaaagtag agaagaaaat ccagtttgct tcttggagaa
      361 cactggacag ctgaataaat gcagtatcta aatataaaag aggactgcaa tgccatggct
      421 ttctgtgcta aaatgaggag ctccaagaag actgaggtga acctggaggc ccctgagcca
      481 ggggtggaag tgatcttcta tctgtcggac agggagcccc tccggctggg cagtggagag
      541 tacacagcag aggaactgtg catcagggct gcacaggcat gccgtatctc tcctctttgt
      601 cacaacctct ttgccctgta tgacgagaac accaagctct ggtatgctcc aaatcgcacc
      661 atcaccgttg atgacaagat gtccctccgg ctccactacc ggatgaggtt ctatttcacc
      721 aattggcatg gaaccaacga caatgagcag tcagtgtggc gtcattctcc aaagaagcag
      781 aaaaatggct acgagaaaaa aaagattcca gatgcaaccc ctctccttga tgccagctca
      841 ctggagtatc tgtttgctca gggacagtat gatttggtga aatgcctggc tcctattcga
      901 gaccccaaga ccgagcagga tggacatgat attgagaacg agtgtctagg gatggctgtc
      961 ctggccatct cacactatgc catgatgaag aagatgcagt tgccagaact gcccaaggac
     1021 atcagctaca agcgatatat tccagaaaca ttgaataagt ccatcagaca gaggaacctt
     1081 ctcaccagga tgcggataaa taatgttttc aaggatttcc taaaggaatt taacaacaag
     1141 accatttgtg acagcagcgt gtccacgcat gacctgaagg tgaaatactt ggctaccttg
     1201 gaaactttga caaaacatta cggtgctgaa atatttgaga cttccatgtt actgatttca
     1261 tcagaaaatg agatgaattg gtttcattcg aatgacggtg gaaacgttct ctactacgaa
     1321 gtgatggtga ctgggaatct tggaatccag tggaggcata aaccaaatgt tgtttctgtt
     1381 gaaaaggaaa aaaataaact gaagcggaaa aaactggaaa ataaacacaa gaaggatgag
     1441 gagaaaaaca agatccggga agagtggaac aatttttctt acttccctga aatcactcac
     1501 attgtaataa aggagtctgt ggtcagcatt aacaagcagg acaacaagaa aatggaactg
     1561 aagctctctt cccacgagga ggccttgtcc tttgtgtccc tggtagatgg ctacttccgg
     1621 ctcacagcag atgcccatca ttacctctgc accgacgtgg cccccccgtt gatcgtccac
     1681 aacatacaga atggctgtca tggtccaatc tgtacagaat acgccatcaa taaattgcgg
     1741 caagaaggaa gcgaggaggg gatgtacgtg ctgaggtgga gctgcaccga ctttgacaac
     1801 atcctcatga ccgtcacctg ctttgagaag tctgagcagg tgcagggtgc ccagaagcag
     1861 ttcaagaact ttcagatcga ggtgcagaag ggccgctaca gtctgcacgg ttcggaccgc
     1921 agcttcccca gcttgggaga cctcatgagc cacctcaaga agcagatcct gcgcacggat
     1981 aacatcagct tcatgctaaa acgctgctgc cagcccaagc cccgagaaat ctccaacctg
     2041 ctggtggcta ctaagaaagc ccaggagtgg cagcccgtct accccatgag ccagctgagt
     2101 ttcgatcgga tcctcaagaa ggatctggtg cagggcgagc accttgggag aggcacgaga
     2161 acacacatct attctgggac cctgatggat tacaaggatg acgaaggaac ttctgaagag
     2221 aagaagataa aagtgatcct caaagtctta gaccccagcc acagggatat ttccctggcc
     2281 ttcttcgagg cagccagcat gatgagacag gtctcccaca aacacatcgt gtacctctat
     2341 ggcgtctgtg tccgcgacgt ggagaatatc atggtggaag agtttgtgga agggggtcct
     2401 ctggatctct tcatgcaccg gaaaagcgat gtccttacca caccatggaa attcaaagtt
     2461 gccaaacagc tggccagtgc cctgagctac ttggaggata aagacctggt ccatggaaat
     2521 gtgtgtacta aaaacctcct cctggcccgt gagggcatcg acagtgagtg tggcccattc
     2581 atcaagctca gtgaccccgg catccccatt acggtgctgt ctaggcaaga atgcattgaa
     2641 cgaatcccat ggattgctcc tgagtgtgtt gaggactcca agaacctgag tgtggctgct
     2701 gacaagtgga gctttggaac cacgctctgg gaaatctgct acaatggcga gatccccttg
     2761 aaagacaaga cgctgattga gaaagagaga ttctatgaaa gccggtgcag gccagtgaca
     2821 ccatcatgta aggagctggc tgacctcatg acccgctgca tgaactatga ccccaatcag
     2881 aggcctttct tccgagccat catgagagac attaataagc ttgaagagca gaatccagat
     2941 attgtttcag aaaaaaaacc agcaactgaa gtggacccca cacattttga aaagcgcttc
     3001 ctaaagagga tccgtgactt gggagagggc cactttggga aggttgagct ctgcaggtat
     3061 gaccccgaag gggacaatac aggggagcag gtggctgtta aatctctgaa gcctgagagt
     3121 ggaggtaacc acatagctga tctgaaaaag gaaatcgaga tcttaaggaa cctctatcat
     3181 gagaacattg tgaagtacaa aggaatctgc acagaagacg gaggaaatgg tattaagctc
     3241 atcatggaat ttctgccttc gggaagcctt aaggaatatc ttccaaagaa taagaacaaa
     3301 ataaacctca aacagcagct aaaatatgcc gttcagattt gtaaggggat ggactatttg
     3361 ggttctcggc aatacgttca ccgggacttg gcagcaagaa atgtccttgt tgagagtgaa
     3421 caccaagtga aaattggaga cttcggttta accaaagcaa ttgaaaccga taaggagtat
     3481 tacaccgtca aggatgaccg ggacagccct gtgttttggt atgctccaga atgtttaatg
     3541 caatctaaat tttatattgc ctctgacgtc tggtcttttg gagtcactct gcatgagctg
     3601 ctgacttact gtgattcaga ttctagtccc atggctttgt tcctgaaaat gataggccca
     3661 acccatggcc agatgacagt cacaagactt gtgaatacgt taaaagaagg aaaacgcctg
     3721 ccgtgcccac ctaactgtcc agatgaggtt tatcaactta tgaggaaatg ctgggaattc
     3781 caaccatcca atcggacaag ctttcagaac cttattgaag gatttgaagc acttttaaaa
     3841 taagaagcat gaataacatt taaattccac agattatcaa gtccttctcc tgcaacaaat
     3901 gcccaagtca ttttttaaaa atttctaatg aaagaagttt gtgttctgtc caaaaagtca
     3961 ctgaactcat acttcagtac atatacatgt ataaggcaca ctgtagtgct taatatgtgt
     4021 aaggacttcc tctttaaatt tggtaccagt aacttagtga cacataatga caaccaaaat
     4081 atttgaaagc acttaagcac tcctccttgt ggaaagaata taccaccatt tcatctggct
     4141 agttcaccat cacaactgca ttaccaaaag gggatttttg aaaacgagga gttgaccaaa
     4201 ataatatctg aagatgattg cttttccctg ctgccagctg atctgaaatg ttttgctggc
     4261 acattaatca tagataaaga aagattgatg gacttagccc tcaaatttca gtatctatac
     4321 agtactagac catgcattct taaaatatta gataccaggt agtatatatt gtttctgtac
     4381 aaaaatgact gtattctctc accagtagga cttaaacttt gtttctccag tggcttagct
     4441 cctgttcctt tgggtgatca ctagcaccca tttttgagaa agctggttct acatgggggg
     4501 atagctgtgg aatagataat ttgctgcatg ttaattctca agaactaagc ctgtgccagt
     4561 gctttcctaa gcagtatacc tttaatcaga actcattccc agaacctgga tgctattaca
     4621 catgctttta agaaacgtca atgtatatcc ttttataact ctaccacttt ggggcaagct
     4681 attccagcac tggttttgaa tgctgtatgc aaccagtctg aataccacat acgctgcact
     4741 gttcttagag ggtttccata cttaccaccg atctacaagg gttgatccct gtttttacca
     4801 tcaatcatca ccctgtggtg caacacttga aagacccggc tagaggcact atggacttca
     4861 ggatccacta gacagttttc agtttgcttg gaggtagctg ggtaatcaaa aatgtttagt
     4921 cattgattca atgtgaacga ttacggtctt tatgaccaag agtctgaaaa tctttttgtt
     4981 atgctgttta gtattcgttt gatattgtta cttttcacct gttgagccca aattcaggat
     5041 tggttcagtg gcagcaatga agttgccatt taaatttgtt catagcctac atcaccaagg
     5101 tctctgtgtc aaacctgtgg ccactctata tgcactttgt ttactcttta tacaaataaa
     5161 tatactaaag acttta
//
