LOCUS       NM_000214               5940 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens jagged canonical Notch ligand 1 (JAG1), mRNA.
ACCESSION   NM_000214
VERSION     NM_000214.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Keefer-Jacques,E., Valente,N., Jacko,A.M., Matwijec,G., Reese,A.,
            Tekriwal,A., Loomes,K.M., Spinner,N.B. and Gilbert,M.A.
  TITLE     Investigation of cryptic JAG1 splice variants as a cause of
            Alagille syndrome and performance evaluation of splice predictor
            tools
  JOURNAL   HGG Adv 5 (4), 100351 (2024)
   PUBMED   39244638
  REMARK    GeneRIF: Investigation of cryptic JAG1 splice variants as a cause
            of Alagille syndrome and performance evaluation of splice predictor
            tools.
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Wu,X., Zhu,H., Liu,J., Ouyang,S., Lyu,Z., Jin,Y., Chen,X. and
            Meng,Q.
  TITLE     Jagged1-Notch1 Signaling Pathway Induces M1 Microglia to Disrupt
            the Barrier Function of Retinal Microvascular Endothelial Cells
  JOURNAL   Curr Eye Res 49 (10), 1098-1106 (2024)
   PUBMED   38783634
  REMARK    GeneRIF: Jagged1-Notch1 Signaling Pathway Induces M1 Microglia to
            Disrupt the Barrier Function of Retinal Microvascular Endothelial
            Cells.
REFERENCE   3  (bases 1 to 5940)
  AUTHORS   Xiao,X., Wang,W., Guo,C., Wu,J., Zhang,S., Shi,H., Kwon,S., Chen,J.
            and Dong,Z.
  TITLE     Hypermethylation leads to the loss of HOXA5, resulting in JAG1
            expression and NOTCH signaling contributing to kidney fibrosis
  JOURNAL   Kidney Int 106 (1), 98-114 (2024)
   PUBMED   38521405
  REMARK    GeneRIF: Hypermethylation leads to the loss of HOXA5, resulting in
            JAG1 expression and NOTCH signaling contributing to kidney
            fibrosis.
REFERENCE   4  (bases 1 to 5940)
  AUTHORS   Huang,H., Jiang,Y., Liu,J., Luo,D., Yuan,J., Mu,R., Yu,X., Sun,D.,
            Lin,J., Chen,Q., Li,X., Jiang,M., Xu,J., Chu,B., Yin,C., Zhang,L.,
            Ye,Y., Cao,B., Wang,Q. and Zhang,Y.
  TITLE     Jag1/2 maintain esophageal homeostasis and suppress foregut
            tumorigenesis by restricting the basal progenitor cell pool
  JOURNAL   Nat Commun 15 (1), 4124 (2024)
   PUBMED   38750026
  REMARK    GeneRIF: Jag1/2 maintain esophageal homeostasis and suppress
            foregut tumorigenesis by restricting the basal progenitor cell
            pool.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5940)
  AUTHORS   Feng,X., Ping,J., Gao,S., Han,D., Song,W., Li,X., Tao,Y. and
            Wang,L.
  TITLE     Novel JAG1 variants leading to Alagille syndrome in two Chinese
            cases
  JOURNAL   Sci Rep 14 (1), 1812 (2024)
   PUBMED   38245625
  REMARK    GeneRIF: Novel JAG1 variants leading to Alagille syndrome in two
            Chinese cases.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5940)
  AUTHORS   Zimrin,A.B., Pepper,M.S., McMahon,G.A., Nguyen,F., Montesano,R. and
            Maciag,T.
  TITLE     An antisense oligonucleotide to the notch ligand jagged enhances
            fibroblast growth factor-induced angiogenesis in vitro
  JOURNAL   J Biol Chem 271 (51), 32499-32502 (1996)
   PUBMED   8955070
REFERENCE   7  (bases 1 to 5940)
  AUTHORS   Genini,M., Schwalbe,P., Scholl,F.A. and Schafer,B.W.
  TITLE     Isolation of genes differentially expressed in human primary
            myoblasts and embryonal rhabdomyosarcoma
  JOURNAL   Int J Cancer 66 (4), 571-577 (1996)
   PUBMED   8635876
REFERENCE   8  (bases 1 to 5940)
  AUTHORS   Lindsell,C.E., Boulter,J., diSibio,G., Gossler,A. and Weinmaster,G.
  TITLE     Expression patterns of Jagged, Delta1, Notch1, Notch2, and Notch3
            genes identify ligand-receptor pairs that may function in neural
            development
  JOURNAL   Mol Cell Neurosci 8 (1), 14-27 (1996)
   PUBMED   8923452
REFERENCE   9  (bases 1 to 5940)
  AUTHORS   Lindsell,C.E., Shawber,C.J., Boulter,J. and Weinmaster,G.
  TITLE     Jagged: a mammalian ligand that activates Notch1
  JOURNAL   Cell 80 (6), 909-917 (1995)
   PUBMED   7697721
REFERENCE   10 (bases 1 to 5940)
  AUTHORS   Spinner,N.B., Loomes,K.M., Krantz,I.D. and Gilbert,M.A.
  TITLE     Alagille Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301450
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF056748.1, U73936.1,
            AF028593.1, AA933912.1 and AI268246.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 8, 2018 this sequence version replaced NM_000214.2.
            
            Summary: The jagged 1 protein encoded by JAG1 is the human homolog
            of the Drosophilia jagged protein. Human jagged 1 is the ligand for
            the receptor notch 1, the latter is involved in signaling
            processes. Mutations that alter the jagged 1 protein cause Alagille
            syndrome. Jagged 1 signalling through notch 1 has also been shown
            to play a role in hematopoiesis. [provided by RefSeq, Nov 2019].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF003837.1, BC098393.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254958.10/ ENSP00000254958.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                BF056748.1         298-381             c
            85-206              U73936.1           29-150
            207-5331            AF028593.1         1-5125
            5332-5479           AA933912.1         176-323             c
            5480-5646           AF028593.1         5273-5439
            5647-5940           AI268246.1         2-295               c
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.2"
     gene            1..5940
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /note="jagged canonical Notch ligand 1"
                     /db_xref="GeneID:182"
                     /db_xref="HGNC:HGNC:6188"
                     /db_xref="MIM:601920"
     exon            1..550
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             470..4126
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /note="protein jagged-1"
                     /codon_start=1
                     /product="protein jagged-1 precursor"
                     /protein_id="NP_000205.1"
                     /db_xref="CCDS:CCDS13112.1"
                     /db_xref="GeneID:182"
                     /db_xref="HGNC:HGNC:6188"
                     /db_xref="MIM:601920"
                     /translation="MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQN
                     VNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVI
                     GGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGM
                     INPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNK
                     TCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGIC
                     NEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCE
                     IAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDL
                     VNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDIN
                     INDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINR
                     FQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDH
                     CRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGT
                     YCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTC
                     RDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTC
                     NIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCV
                     DGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQ
                     EVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSG
                     QSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSP
                     GLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKE
                     ITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTA
                     FYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKM
                     SKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRD
                     LESAQSLNRMEYIV"
     exon            551..856
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..908
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1163
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1224
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1355
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1475
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1589
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1703
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1817
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1864
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2038
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2189
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2354
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2468
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2582
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2696
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2813
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2814..2841
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..2927
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..3041
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3151
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3385
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..3517
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3518..3668
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            3669..5940
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5625..5630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /note="hexamer: ATTAAA"
     polyA_site      5650
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
     regulatory      5916..5921
                     /regulatory_class="polyA_signal_sequence"
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /note="hexamer: AATAAA"
     polyA_site      5940
                     /gene="JAG1"
                     /gene_synonym="AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE;
                     HJ1; JAGL1"
                     /note="major polyA site"
ORIGIN      
        1 agacgggctc tccgggtcct tctccgagag ccgggcgggc acgcgtcatt gtgttacctg
       61 cggccggccc gcgagctagg ctggtttttt tttttctccc ctccctcccc cctttttcca
      121 tgcagctgat ctaaaaggga ataaaaggct gcgcataatc ataataataa aagaagggga
      181 gcgcgagaga aggaaagaaa gccgggaggt ggaagaggag ggggagcgtc tcaaagaagc
      241 gatcagaata ataaaaggag gccgggctct ttgccttctg gaacgggccg ctcttgaaag
      301 ggcttttgaa aagtggtgtt gttttccagt cgtgcatgct ccaatcggcg gagtatatta
      361 gagccgggac gcggcggccg caggggcagc ggcgacggca gcaccggcgg cagcaccagc
      421 gcgaacagca gcggcggcgt cccgagtgcc cgcggcgcgc ggcgcagcga tgcgttcccc
      481 acggacgcgc ggccggtccg ggcgccccct aagcctcctg ctcgccctgc tctgtgccct
      541 gcgagccaag gtgtgtgggg cctcgggtca gttcgagttg gagatcctgt ccatgcagaa
      601 cgtgaacggg gagctgcaga acgggaactg ctgcggcggc gcccggaacc cgggagaccg
      661 caagtgcacc cgcgacgagt gtgacacata cttcaaagtg tgcctcaagg agtatcagtc
      721 ccgcgtcacg gccggggggc cctgcagctt cggctcaggg tccacgcctg tcatcggggg
      781 caacaccttc aacctcaagg ccagccgcgg caacgaccgc aaccgcatcg tgctgccttt
      841 cagtttcgcc tggccgaggt cctatacgtt gcttgtggag gcgtgggatt ccagtaatga
      901 caccgttcaa cctgacagta ttattgaaaa ggcttctcac tcgggcatga tcaaccccag
      961 ccggcagtgg cagacgctga agcagaacac gggcgttgcc cactttgagt atcagatccg
     1021 cgtgacctgt gatgactact actatggctt tggctgcaat aagttctgcc gccccagaga
     1081 tgacttcttt ggacactatg cctgtgacca gaatggcaac aaaacttgca tggaaggctg
     1141 gatgggcccc gaatgtaaca gagctatttg ccgacaaggc tgcagtccta agcatgggtc
     1201 ttgcaaactc ccaggtgact gcaggtgcca gtacggctgg caaggcctgt actgtgataa
     1261 gtgcatccca cacccgggat gcgtccacgg catctgtaat gagccctggc agtgcctctg
     1321 tgagaccaac tggggcggcc agctctgtga caaagatctc aattactgtg ggactcatca
     1381 gccgtgtctc aacgggggaa cttgtagcaa cacaggccct gacaaatatc agtgttcctg
     1441 ccctgagggg tattcaggac ccaactgtga aattgctgag cacgcctgcc tctctgatcc
     1501 ctgtcacaac agaggcagct gtaaggagac ctccctgggc tttgagtgtg agtgttcccc
     1561 aggctggacc ggccccacat gctctacaaa cattgatgac tgttctccta ataactgttc
     1621 ccacgggggc acctgccagg acctggttaa cggatttaag tgtgtgtgcc ccccacagtg
     1681 gactgggaaa acgtgccagt tagatgcaaa tgaatgtgag gccaaacctt gtgtaaacgc
     1741 caaatcctgt aagaatctca ttgccagcta ctactgcgac tgtcttcccg gctggatggg
     1801 tcagaattgt gacataaata ttaatgactg ccttggccag tgtcagaatg acgcctcctg
     1861 tcgggatttg gttaatggtt atcgctgtat ctgtccacct ggctatgcag gcgatcactg
     1921 tgagagagac atcgatgaat gtgccagcaa cccctgtttg aatgggggtc actgtcagaa
     1981 tgaaatcaac agattccagt gtctgtgtcc cactggtttc tctggaaacc tctgtcagct
     2041 ggacatcgat tattgtgagc ctaatccctg ccagaacggt gcccagtgct acaaccgtgc
     2101 cagtgactat ttctgcaagt gccccgagga ctatgagggc aagaactgct cacacctgaa
     2161 agaccactgc cgcacgaccc cctgtgaagt gattgacagc tgcacagtgg ccatggcttc
     2221 caacgacaca cctgaagggg tgcggtatat ttcctccaac gtctgtggtc ctcacgggaa
     2281 gtgcaagagt cagtcgggag gcaaattcac ctgtgactgt aacaaaggct tcacgggaac
     2341 atactgccat gaaaatatta atgactgtga gagcaaccct tgtagaaacg gtggcacttg
     2401 catcgatggt gtcaactcct acaagtgcat ctgtagtgac ggctgggagg gggcctactg
     2461 tgaaaccaat attaatgact gcagccagaa cccctgccac aatgggggca cgtgtcgcga
     2521 cctggtcaat gacttctact gtgactgtaa aaatgggtgg aaaggaaaga cctgccactc
     2581 acgtgacagt cagtgtgatg aggccacgtg caacaacggt ggcacctgct atgatgaggg
     2641 ggatgctttt aagtgcatgt gtcctggcgg ctgggaagga acaacctgta acatagcccg
     2701 aaacagtagc tgcctgccca acccctgcca taatgggggc acatgtgtgg tcaacggcga
     2761 gtcctttacg tgcgtctgca aggaaggctg ggaggggccc atctgtgctc agaataccaa
     2821 tgactgcagc cctcatccct gttacaacag cggcacctgt gtggatggag acaactggta
     2881 ccggtgcgaa tgtgccccgg gttttgctgg gcccgactgc agaataaaca tcaatgaatg
     2941 ccagtcttca ccttgtgcct ttggagcgac ctgtgtggat gagatcaatg gctaccggtg
     3001 tgtctgccct ccagggcaca gtggtgccaa gtgccaggaa gtttcaggga gaccttgcat
     3061 caccatgggg agtgtgatac cagatggggc caaatgggat gatgactgta atacctgcca
     3121 gtgcctgaat ggacggatcg cctgctcaaa ggtctggtgt ggccctcgac cttgcctgct
     3181 ccacaaaggg cacagcgagt gccccagcgg gcagagctgc atccccatcc tggacgacca
     3241 gtgcttcgtc cacccctgca ctggtgtggg cgagtgtcgg tcttccagtc tccagccggt
     3301 gaagacaaag tgcacctctg actcctatta ccaggataac tgtgcgaaca tcacatttac
     3361 ctttaacaag gagatgatgt caccaggtct tactacggag cacatttgca gtgaattgag
     3421 gaatttgaat attttgaaga atgtttccgc tgaatattca atctacatcg cttgcgagcc
     3481 ttccccttca gcgaacaatg aaatacatgt ggccatttct gctgaagata tacgggatga
     3541 tgggaacccg atcaaggaaa tcactgacaa aataatcgat cttgttagta aacgtgatgg
     3601 aaacagctcg ctgattgctg ccgttgcaga agtaagagtt cagaggcggc ctctgaagaa
     3661 cagaacagat ttccttgttc ccttgctgag ctctgtctta actgtggctt ggatctgttg
     3721 cttggtgacg gccttctact ggtgcctgcg gaagcggcgg aagccgggca gccacacaca
     3781 ctcagcctct gaggacaaca ccaccaacaa cgtgcgggag cagctgaacc agatcaaaaa
     3841 ccccattgag aaacatgggg ccaacacggt ccccatcaag gattatgaga acaagaactc
     3901 caaaatgtct aaaataagga cacacaattc tgaagtagaa gaggacgaca tggacaaaca
     3961 ccagcagaaa gcccggtttg ccaagcagcc ggcgtacacg ctggtagaca gagaagagaa
     4021 gccccccaac ggcacgccga caaaacaccc aaactggaca aacaaacagg acaacagaga
     4081 cttggaaagt gcccagagct taaaccgaat ggagtacatc gtatagcaga ccgcgggcac
     4141 tgccgccgct aggtagagtc tgagggcttg tagttcttta aactgtcgtg tcatactcga
     4201 gtctgaggcc gttgctgact tagaatccct gtgttaattt aagttttgac aagctggctt
     4261 acactggcaa tggtagtttc tgtggttggc tgggaaatcg agtgccgcat ctcacagcta
     4321 tgcaaaaagc tagtcaacag taccctggtt gtgtgtcccc ttgcagccga cacggtctcg
     4381 gatcaggctc ccaggagcct gcccagcccc ctggtctttg agctcccact tctgccagat
     4441 gtcctaatgg tgatgcagtc ttagatcata gttttattta tatttattga ctcttgagtt
     4501 gtttttgtat attggtttta tgatgacgta caagtagttc tgtatttgaa agtgcctttg
     4561 cagctcagaa ccacagcaac gatcacaaat gactttatta tttatttttt ttaattgtat
     4621 ttttgttgtt gggggagggg agactttgat gtcagcagtt gctggtaaaa tgaagaattt
     4681 aaagaaaaaa atgtcaaaag tagaactttg tatagttatg taaataattc ttttttatta
     4741 atcactgtgt atatttgatt tattaactta ataatcaaga gccttaaaac atcattcctt
     4801 tttatttata tgtatgtgtt tagaattgaa ggtttttgat agcattgtaa gcgtatggct
     4861 ttattttttt gaactcttct cattacttgt tgcctataag ccaaaattaa ggtgtttgaa
     4921 aatagtttat tttaaaacaa taggatgggc ttctgtgccc agaatactga tggaattttt
     4981 ttgtacgacg tcagatgttt aaaacacctt ctatagcatc acttaaaaca cgttttaagg
     5041 actgactgag gcagtttgag gattagttta gaacaggttt ttttgtttgt ttgttttttg
     5101 tttttctgct ttagacttga aaagagacag gcaggtgatc tgctgcagag cagtaaggga
     5161 acaagttgag ctatgactta acatagccaa aatgtgagtg gttgaatatg attaaaaata
     5221 tcaaattaat tgtgtgaact tggaagcaca ccaatcttac tttgtaaatt ctgatttctt
     5281 ttcaccattc gtacataata ctgaaccact tgtagatttg attttttttt ttaatctact
     5341 gcatttaggg agtattctaa taagctagtt gaatacttga accataaaat gtccagtaag
     5401 atcactgttt agatttgcca tagagtacac tgcctgcctt aagtgaggaa atcaaagtgc
     5461 tattacgaag ttcaagatca aaaaggctta taaaacagag taatcttgtt ggttcaccat
     5521 tgagaccgtg aagatacttt gtattgtcct attagtgtta tatgaacata caaatgcatc
     5581 tttgatgtgt tgttcttggc aataaatttt gaaaagtaat atttattaaa tttttttgta
     5641 tgaaaacatg gaacagtgtg gcctcttctg agcttacgta gttctaccgg ctttgccatg
     5701 tgcttctgcc accctgctga gtctgttctg gtaatcgggg tataataggc tctgcctgac
     5761 agagggatgg aggaagaact gaaaggcttt tcaaccacaa aactcatctg gagttctcaa
     5821 agacctgggg ctgctgtgaa gctggaactg cgggagcccc atctagggga gccttgattc
     5881 ccttgttatt caacagcaag tgtgaatact gcttgaataa acaccactgg attaatggcc
//
