LOCUS       NM_017969               2973 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens interacts with SUPT6H, CTD assembly factor 1 (IWS1),
            transcript variant 1, mRNA.
ACCESSION   NM_017969
VERSION     NM_017969.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Cermakova,K., Demeulemeester,J., Lux,V., Nedomova,M., Goldman,S.R.,
            Smith,E.A., Srb,P., Hexnerova,R., Fabry,M., Madlikova,M.,
            Horejsi,M., De Rijck,J., Debyser,Z., Adelman,K., Hodges,H.C. and
            Veverka,V.
  TITLE     A ubiquitous disordered protein interaction module orchestrates
            transcription elongation
  JOURNAL   Science 374 (6571), 1113-1121 (2021)
   PUBMED   34822292
  REMARK    GeneRIF: A ubiquitous disordered protein interaction module
            orchestrates transcription elongation.
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Sharma,S., Cermakova,K., De Rijck,J., Demeulemeester,J., Fabry,M.,
            El Ashkar,S., Van Belle,S., Lepsik,M., Tesina,P., Duchoslav,V.,
            Novak,P., Hubalek,M., Srb,P., Christ,F., Rezacova,P., Hodges,H.C.,
            Debyser,Z. and Veverka,V.
  TITLE     Affinity switching of the LEDGF/p75 IBD interactome is governed by
            kinase-dependent phosphorylation
  JOURNAL   Proc Natl Acad Sci U S A 115 (30), E7053-E7062 (2018)
   PUBMED   29997176
REFERENCE   3  (bases 1 to 2973)
  AUTHORS   Tesina,P., Cermakova,K., Horejsi,M., Prochazkova,K., Fabry,M.,
            Sharma,S., Christ,F., Demeulemeester,J., Debyser,Z., Rijck,J.,
            Veverka,V. and Rezacova,P.
  TITLE     Multiple cellular proteins interact with LEDGF/p75 through a
            conserved unstructured consensus motif
  JOURNAL   Nat Commun 6, 7968 (2015)
   PUBMED   26245978
  REMARK    GeneRIF: common LEDGF/p75 interaction interface shared by JPO2,
            PogZ, MLL1, IWS1 and HIV IN
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2973)
  AUTHORS   Gao,K., Xu,C., Jin,X., Wumaier,R., Ma,J., Peng,J., Wang,Y.,
            Tang,Y., Yu,L. and Zhang,P.
  TITLE     HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell
            growth in hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 458 (4), 849-855 (2015)
   PUBMED   25689719
REFERENCE   5  (bases 1 to 2973)
  AUTHORS   Gerard,A., Segeral,E., Naughtin,M., Abdouni,A., Charmeteau,B.,
            Cheynier,R., Rain,J.C. and Emiliani,S.
  TITLE     The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for
            postintegration silencing of HIV-1 gene expression in latently
            infected cells
  JOURNAL   Cell Host Microbe 17 (1), 107-117 (2015)
   PUBMED   25590759
  REMARK    GeneRIF: Altogether, these results indicate that a complex
            containing LEDGF/p75, Iws1, and Spt6 participates in regulating
            postintegration steps of HIV latency.
REFERENCE   6  (bases 1 to 2973)
  AUTHORS   Liu,Z., Zhou,Z., Chen,G. and Bao,S.
  TITLE     A putative transcriptional elongation factor hIws1 is essential for
            mammalian cell proliferation
  JOURNAL   Biochem Biophys Res Commun 353 (1), 47-53 (2007)
   PUBMED   17184735
  REMARK    GeneRIF: Here we report that the human homolog of Iws1, hIws1,
            physically interacts with protein arginine methyltransferases PRMT5
            which methylates elongation factor Spt5 and regulates its
            interaction with RNA polymerase II.
REFERENCE   7  (bases 1 to 2973)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   8  (bases 1 to 2973)
  AUTHORS   Ling,Y., Smith,A.J. and Morgan,G.T.
  TITLE     A sequence motif conserved in diverse nuclear proteins identifies a
            protein interaction domain utilised for nuclear targeting by human
            TFIIS
  JOURNAL   Nucleic Acids Res 34 (8), 2219-2229 (2006)
   PUBMED   16648364
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2973)
  AUTHORS   Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R.
  TITLE     Phosphoproteome analysis of the human mitotic spindle
  JOURNAL   Proc Natl Acad Sci U S A 103 (14), 5391-5396 (2006)
   PUBMED   16565220
REFERENCE   10 (bases 1 to 2973)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA268882.1, BC110537.1 and AI638093.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017969.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.123876.1,
                                           SRR1803617.157757.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295321.9/ ENSP00000295321.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               DA268882.1         24-171
            149-2851            BC110537.1         1-2703
            2852-2973           AI638093.1         6-127               c
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.3"
     gene            1..2973
                     /gene="IWS1"
                     /note="interacts with SUPT6H, CTD assembly factor 1"
                     /db_xref="GeneID:55677"
                     /db_xref="HGNC:HGNC:25467"
     exon            1..315
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             282..2741
                     /gene="IWS1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     interacts with Spt6; protein IWS1 homolog; IWS1-like
                     protein; IWS1, SUPT6H interacting protein"
                     /codon_start=1
                     /product="protein IWS1 homolog isoform 1"
                     /protein_id="NP_060439.2"
                     /db_xref="CCDS:CCDS2146.1"
                     /db_xref="GeneID:55677"
                     /db_xref="HGNC:HGNC:25467"
                     /translation="MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGS
                     VERHSENETSDREDGLPKGHHVTDSENDEPLNLNASDSESEELHRQKDSDSESEERAE
                     PPASDSENEDVNQHGSDSESEETRKLPGSDSENEELLNGHASDSENEDVGKHPASDSE
                     IEELQKSPASDSETEDALKPQISDSESEEPPRHQASDSENEEPPKPRMSDSESEELPK
                     PQVSDSESEEPPRHQASDSENEELPKPRISDSESEDPPRHQASDSENEELPKPRISDS
                     ESEDPPRNQASDSENEELPKPRVSDSESEGPQKGPASDSETEDASRHKQKPESDDDSD
                     RENKGEDTEMQNDSFHSDSHMDRKKFHSSDSEEEEHKKQKMDSDEDEKEGEEEKVAKR
                     KAAVLSDSEDEEKASAKKSRVVSDADDSDSDAVSDKSGKREKTIASDSEEEAGKELSD
                     KKNEEKDLFGSDSESGNEEENLIADIFGESGDEEEEEFTGFNQEDLEEEKGETQVKEA
                     EDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVVSAMIVK
                     MNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLP
                     DRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLI
                     NEWSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRMNSTGGQTPRRDLEKVLTGEEKA
                     LRPGDPGFCARARVPMPSNKDYVVRPKWNVEMESSRFQATSKKGISRLDKQMRKFTDI
                     RKKSRSAHAVKISIEGNKMPL"
     exon            316..431
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..1500
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1690
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1748
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1846
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1997
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2080
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2210
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2328
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2440
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2497
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2498..2609
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2973
                     /gene="IWS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2954..2959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IWS1"
                     /note="hexamer: AATAAA"
     polyA_site      2973
                     /gene="IWS1"
                     /note="major polyA site"
ORIGIN      
        1 agacgccatt ttggccccag cggtgcttct aggaagtaga acgccggctc gcatgcctgc
       61 ccgcccgcca gcctgccggg tacggccttt tccgccgggg cttccaggtc aaagaattcg
      121 cctttgccgc taccgctttc ttaccctccg cacccgttaa gttctccggt cgggcggcag
      181 tctctgaaca cttagccgcg ccatccgggg tcacaccgcc tggaaggagg tgacgggggc
      241 ggcgcggggc gcggacactc cccgctgaga gtccgcctgc catggactcg gaatattaca
      301 gcggcgacca gtcagatgat ggtggtgcta ccccagtaca ggatgaacgg gattcagggt
      361 cagacggtga ggatgatgta aatgagcaac actccggatc agacactgga agtgtagaac
      421 gtcattcaga gaatgaaact agtgatcgag aagatggcct ccccaaagga catcatgtga
      481 cagactctga gaacgatgag cccttaaatc ttaatgctag tgactctgaa agtgaggagc
      541 ttcacaggca aaaggacagc gactctgaat ctgaggaacg tgcagagcct cctgcaagcg
      601 attctgaaaa tgaggatgtc aatcagcatg ggagcgactc tgagagtgaa gagaccagga
      661 aattacctgg tagtgactct gaaaatgagg aacttcttaa tgggcatgca agtgactcag
      721 aaaacgaaga tgttgggaag catcccgcca gtgattctga gattgaggag ctccagaaga
      781 gtcctgctag tgactctgaa acagaagatg ctctaaaacc tcaaatcagt gactctgaga
      841 gtgaggaacc cccaaggcac caagccagtg actccgaaaa tgaggagcct cccaaacctc
      901 gaatgagtga ttctgaaagt gaggagcttc ctaaacctca ggtcagtgat tcagaaagtg
      961 aggaaccccc aaggcaccag gccagtgact ctgaaaatga ggagcttccc aaacctcgta
     1021 tcagtgactc agaaagtgag gaccctccga ggcaccaggc cagtgactca gaaaatgaag
     1081 agcttcccaa accccgaatc agtgattcgg aaagtgagga tcccccaagg aaccaggcca
     1141 gtgattcgga aaatgaggag ctacccaaac cccgagtcag tgactctgag agtgaggggc
     1201 ctcagaaggg gcctgccagt gactcagaaa ctgaggatgc gtccagacac aaacagaagc
     1261 cagagtcaga tgatgacagc gacagggaga ataagggaga ggatacagaa atgcagaatg
     1321 actccttcca ttcagacagc catatggaca gaaaaaagtt tcacagttct gatagtgagg
     1381 aggaagaaca caaaaagcaa aaaatggaca gtgatgaaga tgaaaaagag ggtgaggagg
     1441 agaaagtagc gaagagaaaa gctgctgtgc tttctgatag tgaagatgaa gagaaagcat
     1501 cagcaaagaa gagtcgtgtt gtctctgatg cagatgactc tgacagtgat gctgtatcag
     1561 acaagtcagg caaaagagag aagaccatag catctgacag tgaggaagaa gctgggaaag
     1621 aattgtctga taagaaaaat gaagagaagg atctgtttgg gagtgacagt gagtcaggca
     1681 atgaagaaga aaatcttatt gcagacatat ttggagaatc tggtgatgaa gaggaagaag
     1741 aatttacagg ttttaaccaa gaagatctgg aagaagaaaa aggtgaaaca caggtaaaag
     1801 aagcagaaga ttcagattct gatgataaca taaagagagg aaaacatatg gactttctgt
     1861 cagattttga gatgatgttg cagcgaaaaa agagcatgag tggcaagcgc agacggaacc
     1921 gcgatggtgg cacctttatt agtgatgcag acgacgtcgt gagtgccatg atcgtcaaga
     1981 tgaatgaagc tgctgaggaa gacagacagt tgaacaatca aaaaaagcca gcactgaaaa
     2041 aattaacttt actgcctgct gtagttatgc accttaagaa gcaggacctt aaagaaacat
     2101 tcattgacag tggtgtgatg tctgccatca aagaatggct ctcacctcta ccagatagga
     2161 gtttgcctgc actcaagatc cgggaggagc tgctgaagat cctgcaagag ctgcctagtg
     2221 tgagccagga gaccctgaag catagtggga ttggacgagc agtgatgtat ctctataaac
     2281 accccaagga gtcaaggtct aacaaggaca tggcagggaa attaatcaat gagtggtcta
     2341 ggcctatatt tggtcttacc tcaaactaca aaggaatgac aagagaagaa agggagcaga
     2401 gagatctaga acagatgcct caacgacgaa gaatgaacag cactggtggt cagacaccca
     2461 gaagagacct ggaaaaggtg ctgacaggag aggagaaggc tcttagacct ggagatcctg
     2521 gattctgtgc ccgtgcaagg gtcccaatgc cttcaaacaa ggactatgtt gtcaggccca
     2581 aatggaatgt ggaaatggag tcatccaggt ttcaggcgac ctccaagaag ggtatcagtc
     2641 gactggataa acagatgaga aagttcacag atataaggaa aaaaagcaga tctgcacacg
     2701 cagtgaaaat cagcattgag ggcaacaaaa tgccattgtg accttgcctg gaatgtgtcc
     2761 ccatctctac tctaagaaat gcgcaatgga ctctttggag aaagaagata ttttaaaaca
     2821 tttttagtgt gtctgtaaat ggttcagcgt gtatcagatg ttgtcatagg actcacattt
     2881 ctctcagtta tatttaaaac cgttgtgtac tttgtacaaa ggaatactag tcatacttct
     2941 ataaacttta cacaataaaa tttcattctg gtt
//
