LOCUS       NM_006277               6122 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens intersectin 2 (ITSN2), transcript variant 1, mRNA.
ACCESSION   NM_006277
VERSION     NM_006277.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Salokas,K., Liu,X., Ohman,T., Chowdhury,I., Gawriyski,L.,
            Keskitalo,S. and Varjosalo,M.
  TITLE     Physical and functional interactome atlas of human receptor
            tyrosine kinases
  JOURNAL   EMBO Rep 23 (6), e54041 (2022)
   PUBMED   35384245
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 6122)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6122)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6122)
  AUTHORS   Kropyvko,S., Gryaznova,T., Morderer,D. and Rynditch,A.
  TITLE     Mammalian verprolin CR16 acts as a modulator of ITSN scaffold
            proteins association with actin
  JOURNAL   Biochem Biophys Res Commun 484 (4), 813-819 (2017)
   PUBMED   28161632
  REMARK    GeneRIF: These findings provide the basis for further functional
            investigations of the ITSN/CR16 complex that may play an important
            role in actin remodeling and cellular invasion.
REFERENCE   6  (bases 1 to 6122)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
REFERENCE   7  (bases 1 to 6122)
  AUTHORS   McGavin,M.K., Badour,K., Hardy,L.A., Kubiseski,T.J., Zhang,J. and
            Siminovitch,K.A.
  TITLE     The intersectin 2 adaptor links Wiskott Aldrich Syndrome protein
            (WASp)-mediated actin polymerization to T cell antigen receptor
            endocytosis
  JOURNAL   J Exp Med 194 (12), 1777-1787 (2001)
   PUBMED   11748279
REFERENCE   8  (bases 1 to 6122)
  AUTHORS   Pucharcos,C., Casas,C., Nadal,M., Estivill,X. and de la Luna,S.
  TITLE     The human intersectin genes and their spliced variants are
            differentially expressed
  JOURNAL   Biochim Biophys Acta 1521 (1-3), 1-11 (2001)
   PUBMED   11690630
REFERENCE   9  (bases 1 to 6122)
  AUTHORS   Pucharcos,C., Estivill,X. and de la Luna,S.
  TITLE     Intersectin 2, a new multimodular protein involved in
            clathrin-mediated endocytosis
  JOURNAL   FEBS Lett 478 (1-2), 43-51 (2000)
   PUBMED   10922467
REFERENCE   10 (bases 1 to 6122)
  AUTHORS   Sparks,A.B., Hoffman,N.G., McConnell,S.J., Fowlkes,D.M. and
            Kay,B.K.
  TITLE     Cloning of ligand targets: systematic isolation of SH3
            domain-containing proteins
  JOURNAL   Nat Biotechnol 14 (6), 741-744 (1996)
   PUBMED   9630982
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009228.4, DA382952.1,
            AF182198.1, BC146779.1 and AC008073.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 3, 2019 this sequence version replaced NM_006277.2.
            
            Summary: This gene encodes a cytoplasmic protein which contains SH3
            domains. This protein is a member of a family of proteins involved
            in clathrin-mediated endocytosis. Intersectin 2 is thought to
            regulate the formation of clathrin-coated vesicles and also may
            function in the induction of T cell antigen receptor (TCR)
            endocytosis. [provided by RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1), also known as ITSN2-L,
            encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF182198.1, SRR14038193.1896682.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355123.9/ ENSP00000347244.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AC009228.4         146149-146156       c
            9-207               DA382952.1         251-449
            208-4605            AF182198.1         179-4576
            4606-4606           BC146779.1         4424-4424
            4607-6120           AF182198.1         4578-6091
            6121-6122           AC008073.4         154944-154945       c
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..6122
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /note="intersectin 2"
                     /db_xref="GeneID:50618"
                     /db_xref="HGNC:HGNC:6184"
                     /db_xref="MIM:604464"
     exon            1..233
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            234..297
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..5360
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /note="isoform 1 is encoded by transcript variant 1; SH3
                     domain-containing protein 1B; SH3P18-like WASP-associated
                     protein"
                     /codon_start=1
                     /product="intersectin-2 isoform 1"
                     /protein_id="NP_006268.2"
                     /db_xref="CCDS:CCDS1710.2"
                     /db_xref="GeneID:50618"
                     /db_xref="HGNC:HGNC:6184"
                     /db_xref="MIM:604464"
                     /translation="MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQ
                     ARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPI
                     MKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVP
                     SVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSS
                     STSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSN
                     LSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGG
                     KQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQRE
                     AERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAA
                     KQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGR
                     LQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIK
                     NMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGN
                     MDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLEL
                     MQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQR
                     KDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLY
                     GSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASV
                     TDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKD
                     NHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVN
                     KKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFP
                     SNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTS
                     GWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDEL
                     SFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQ
                     PIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIM
                     SNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTD
                     EDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSL
                     KLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLL
                     HSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIF
                     LNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKK
                     KREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQD
                     TLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPM
                     TRRLLLHEVPTGEVWVRFDLQLFEQKTLL"
     exon            298..390
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            391..454
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            455..618
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            619..822
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            823..919
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1059
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1123
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1261
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1347
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1610
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1760
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1901
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..1989
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2129
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2210
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2347
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2523
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2621
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2803
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..2948
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..3154
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3219
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3220..3386
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3432
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3433..3554
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3651
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3843
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3844..3965
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3966..4072
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4256
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4257..4355
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4523
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4524..4739
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4740..4861
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4862..4944
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4945..5028
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5029..5202
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5203..6122
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6096..6101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /note="hexamer: AATAAA"
     polyA_site      6122
                     /gene="ITSN2"
                     /gene_synonym="PRO2015; SH3D1B; SH3P18; SWA; SWAP"
                     /note="major polyA site"
ORIGIN      
        1 ggaggccgcg gccgccatgt tgttgtgggg ctgaggcggc gccggcggag ccctgagcgg
       61 ctgtgacagg ctacgcaaca ggttcgcggg cggcggcctg acgaccaagc cagctgcagt
      121 ggcggcgacg gcggcagagc agggtctccc cgcgcctgcc cgcgcccagg ctgccggtgc
      181 tgagggacgc ggagtcgcgc tgtgacgagc gggaggcgcg gcgagggcgc cagatggctg
      241 agagctagca aggaaaactc aggaccatga tggctcagtt tcccacagct atgaatggag
      301 ggccaaacat gtgggctatt acctctgaag aacgtactaa gcatgacagg cagtttgata
      361 acctcaaacc ttcaggaggt tacataacag gtgatcaagc acgtaatttt ttcctacaat
      421 caggtctgcc ggcccctgtt ttagctgaaa tatgggcttt atcagaccta aacaaggatg
      481 ggaagatgga tcagcaagag ttctccatag ctatgaaact catcaaactg aagcttcaag
      541 gccaacagtt gcctgtggtt ctccctccta ttatgaagca accccctatg ttttctccat
      601 taatttctgc tcgttttgga atgggaagca tgcccaatct gtccattcct cagccattgc
      661 ctccagctgc acctataaca tcattgtctt ctgcgacttc agggaccaac cttcctccct
      721 taatgatgcc cactccccta gtgccttctg ttagcacatc atcattacca aatggaaccg
      781 ccagtctcat tcagccttta cccattcctt attcttcttc aacattgcct catgggtcat
      841 cttatagtct gatgatggga ggatttggag gtgctagtat acagaaagcg cagtctctga
      901 ttgatttagg atctagtagc tcaacttcct cgactgcttc actctcaggg aactcaccca
      961 agactgggac ctcagagtgg gcagttcctc agcctacaag attaaaatat cggcaaaaat
     1021 ttaatactct tgacaaaagt atgagtggat atctctcagg ttttcaagct agaaatgccc
     1081 ttcttcagtc aaatctttct caaactcagc tggctactat ttggactctg gctgacgttg
     1141 atggtgatgg acagctaaaa gcagaagagt ttattcttgc aatgcacctt actgacatgg
     1201 ccaaagctgg acagccatta ccactgactt tacctcctga gcttgttcct ccatctttca
     1261 gaggaggaaa gcaaattgat tccattaatg gaactctgcc ttcatatcag aaaatgcaag
     1321 aagaggagcc tcagaagaaa ttaccagtta cttttgagga caaacggaaa gccaactatg
     1381 agcgagggaa catggagctg gaaaagcgac gccaagcctt gatggagcag caacaaaggg
     1441 aggcagaacg taaagcccag aaagaaaagg aagagtggga acgaaaacag agagaattac
     1501 aagaacaaga atggaagaaa caacttgaat tagaaaaacg cttagagaag caacgggaat
     1561 tggagagaca acgagaggaa gaaaggagaa aagacataga aagacgagag gcagcaaaac
     1621 aggaacttga acgacaacgt cgcttagaat gggagagaat tcggcgacag gagcttctca
     1681 atcaaaagaa tagagaacaa gaagaaattg tcaggttaaa ctctaaaaag aagaatcttc
     1741 atcttgagtt ggaagcactg aatggcaaac atcagcagat ctcaggcaga cttcaggatg
     1801 tccgactcaa aaagcaaact caaaagactg agctggaagt tctggataag cagtgtgact
     1861 tggaaattat ggaaatcaag caacttcaac aggaacttca ggaatatcag aataagctta
     1921 tctatctggt acctgagaag caattattaa atgaaagaat taaaaacatg cagttcagta
     1981 acacacctga ttcaggggtc agtttacttc ataaaaaatc attagaaaag gaagaattat
     2041 gccaaagact taaagaacag ttagatgctc ttgaaaaaga aactgcatct aagctgtcag
     2101 aaatggattc ttttaacaat caactaaagt gtgggaatat ggatgactct gttcttcagt
     2161 gccttttgtc tctgctaagc tgtctcaaca acctcttcct cttacttaag gaactgagag
     2221 aaacctacaa cacacagcag ttagcccttg aacagcttta taagatcaaa cgtgacaagt
     2281 tgaaggaaat tgaaaggaaa agattagaac taatgcagaa aaagaaacta gaagatgagg
     2341 ctgcaaggaa agcaaagcaa ggaaaagaaa acttatggaa agaaaatctt agaaaggagg
     2401 aagaagaaaa acaaaagcga ctccaggaag aaaaaacaca agaaaaaatt caagaagagg
     2461 aacggaaagc tgaggagaaa caacgtaagg ataaggatac tttgaaagct gaggagaaaa
     2521 aacgtgagac agctagtgtt ttggtgaatt atagagcatt ataccccttt gaagcaagga
     2581 accatgatga gatgagtttt aattctggag atataattca ggttgatgaa aaaaccgtag
     2641 gagaacctgg ttggctttat ggtagttttc aaggaaattt tggctggttt ccatgcaatt
     2701 atgtagaaaa aatgccatca agtgaaaatg aaaaagctgt atctccaaag aaggccttac
     2761 ttcctcctac agtttcttta tctgctacct caacttcctc tgaaccactt tcttcaaatc
     2821 aaccagcatc agtgactgat tatcaaaatg tatctttttc aaacctaact gtaaatacat
     2881 catggcagaa aaaatcagcc ttcactcgaa ctgtgtcccc tggatctgta tcacctattc
     2941 atggacaggg acaagtggta gaaaacttaa aagcacaggc cctttgttcc tggactgcaa
     3001 agaaagataa ccacttgaac ttctcaaaac atgacattat tactgtcttg gagcagcaag
     3061 aaaattggtg gtttggggag gtgcatggag gaagaggatg gtttcccaaa tcttatgtca
     3121 agatcattcc tgggagtgaa gtaaaacggg aagaaccaga agctttgtat gcagctgtaa
     3181 ataagaaacc tacctcggca gcctattcag ttggagaaga atatattgca ctttatccat
     3241 attcaagtgt ggaacctgga gatttgactt tcacagaagg tgaagaaata ttggtgaccc
     3301 agaaagatgg agagtggtgg acaggaagta ttggagatag aagtggaatt tttccatcaa
     3361 actatgtcaa accaaaggat caagagagtt ttgggagtgc tagcaagtct ggagcatcaa
     3421 ataaaaaacc tgagattgct caggtaactt cagcatatgt tgcttctggt tctgaacaac
     3481 ttagccttgc accaggacag ttaatattaa ttctaaagaa aaatacaagt gggtggtggc
     3541 aaggagagtt acaggccaga ggaaaaaagc gacagaaagg atggtttcct gccagtcatg
     3601 ttaaactttt gggtccaagt agtgaaagag ccacacctgc ctttcatcct gtatgtcagg
     3661 tgattgctat gtatgactat gcagcaaata atgaagatga gctcagtttc tccaagggac
     3721 aactcattaa tgttatgaac aaagatgatc ctgattggtg gcaaggagag atcaacgggg
     3781 tgactggtct ctttccttca aactacgtta agatgacgac agactcagat ccaagtcaac
     3841 agtggtgtgc tgatctgcaa accctggaca caatgcagcc aattgagagg aaaagacagg
     3901 gctatattca tgagctgatt cagaccgaag agcggtacat ggctgacctt cagctcgtcg
     3961 tcgaggtttt tcagaaacgc atggcagagt caggctttct cactgaaggg gagatggccc
     4021 tgatttttgt taactggaag gagctcatca tgtccaacac aaagctgctg aaggctttgc
     4081 gggtgcggaa gaagaccggg ggcgagaaga tgccggtgca gatgattggg gacatcctgg
     4141 ccgctgagct gtcccacatg caggcttaca tcaggttctg cagctgccag cttaatggag
     4201 cagctctgtt acagcagaag acagatgaag acacagattt caaagaattt ttaaagaagc
     4261 tggcatctga cccgcggtgt aaaggaatgc ccctctccag cttcctgctg aaacccatgc
     4321 agaggatcac ccgctaccca ctgctcatca gaagtattct ggagaacacc ccggagagcc
     4381 atgcagacca ttcctcccta aagctggccc tcgagcgggc agaggagctg tgctctcaag
     4441 tgaatgaggg agttcgggag aaggaaaact cggaccgact ggagtggatc caggcgcacg
     4501 tgcagtgtga aggcctcgcg gagcaactta ttttcaactc tctcaccaac tgcctggggc
     4561 cccggaagct cttacacagt gggaaattat acaagaccaa gagcaacaag gaactgcacg
     4621 gattcctctt caatgacttc ctgcttctta cctacatggt caagcagttt gctgtttcct
     4681 ctggctctga gaaacttttc agctcgaagt ccaatgctca attcaaaatg tataaaacgc
     4741 ccattttcct gaatgaagtc ttggtgaaac tgcccacaga cccttccagc gatgagcctg
     4801 tcttccacat ttcccacatt gatcgggtct acaccctccg aacagacaac attaatgaga
     4861 ggaccgcctg ggtgcagaag atcaaggcgg cgtctgagca gtacatcgac accgagaaga
     4921 agaagcgtga gaaagcttac caagcccgct cccaaaagac ttcaggcatt gggcgcctga
     4981 tggtgcatgt cattgaagct acagaattaa aagcctgcaa accaaatgga aagagcaacc
     5041 catactgtga aatcagcatg ggctcccaga gctacaccac caggaccatc caggacacac
     5101 tcaatcccaa gtggaatttt aactgccagt tctttattaa ggatctctac caagacgtgc
     5161 tgtgtctcac cctgtttgac agagaccagt tttcaccaga tgatttcctg ggtcgtactg
     5221 aaattccagt ggcaaaaatt cgaacagaac aggaaagcaa aggccctatg acccgccgac
     5281 tgctgctgca tgaggtcccc accggggagg tctgggtccg ttttgacctg cagctttttg
     5341 agcaaaaaac tctcctgtag gggttctaaa ggacagcacc agcgggacag cccacaaggc
     5401 tggggctgga gaatgagaga ctgcgctctc ttggggctga gggagcacca tgcagcttca
     5461 cccctcacaa agccatgcac gctgggggct ctgttttcct gcacactaaa tagctagcaa
     5521 tctatgcaaa cacctttccc ataaagaaac caaaccccat agtacagtgc cttgtcctag
     5581 tgttcacatg ttcagctctg tttgtttaga tgccaaggtt tccattttca gggctataaa
     5641 aagtattact tggaaatgag gcatcagacc accagatgtt accgctcggt tgaatgtgtc
     5701 caccgtggag tggtttggtg acgctgtaac cattccacgc cagtgacctc tgctgggtca
     5761 cagccactca ggaggggaag ggtcaggatg agaggctgca gcctcgacac ttggcgcggc
     5821 ctgatactga aatagcgtct actcgtgcac tgaataaaaa cagaaacttg atcattttat
     5881 tcctgattag attttatcac tctctgctaa gacaatatag tctggagtat aagtgggaaa
     5941 gcttgattta aatactgtga actctaataa tgtggaaaat atttttcaac tttaattttc
     6001 tgaagtataa attatttatg taaattcatt gtttttgcat atttcttagg acatgcatct
     6061 ttaagcttta tcattgccca tatgtacaga aagagaataa agacatatgt ttatggatgg
     6121 aa
//
