LOCUS       NM_021999              10089 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens integral membrane protein 2B (ITM2B), mRNA.
ACCESSION   NM_021999
VERSION     NM_021999.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Xian,D., Yang,S., Liu,Y., Liu,Q., Huang,D. and Wu,Y.
  TITLE     MicroRNA-196a-5p facilitates the onset and progression via
            targeting ITM2B in esophageal squamous cell carcinoma
  JOURNAL   Pathol Int 74 (3), 129-138 (2024)
   PUBMED   38289121
  REMARK    GeneRIF: MicroRNA-196a-5p facilitates the onset and progression via
            targeting ITM2B in esophageal squamous cell carcinoma.
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Ma,T., Jiang,J., Shi,M. and Xu,H.
  TITLE     Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation
            by targeting ITM3E in colorectal cancer
  JOURNAL   Environ Toxicol 39 (2), 803-814 (2024)
   PUBMED   37792719
  REMARK    GeneRIF: Exosomal miRNA-166-5p derived from G-MDSCs promotes
            proliferation by targeting ITM3E in colorectal cancer.
            Erratum:[Environ Toxicol. 2025 Feb;40(2):357-360. doi:
            10.1002/tox.24422.. PMID: 39301815]
REFERENCE   3  (bases 1 to 10089)
  AUTHORS   Tigro,H., Shimozawa,M., Nilsson,P., Lyashkov,A., Khadeer,M.,
            Jarving,I., Ferrucci,L., Shimmo,R., Johansson,J. and Moaddel,R.
  TITLE     Identification of glycolytic proteins as binding partners of Bri2
            BRICHOS domain
  JOURNAL   J Pharm Biomed Anal 232, 115465 (2023)
   PUBMED   37220701
  REMARK    GeneRIF: Identification of glycolytic proteins as binding partners
            of Bri2 BRICHOS domain.
REFERENCE   4  (bases 1 to 10089)
  AUTHORS   Shimozawa,M., Tigro,H., Biverstal,H., Shevchenko,G., Bergquist,J.,
            Moaddel,R., Johansson,J. and Nilsson,P.
  TITLE     Identification of cytoskeletal proteins as binding partners of Bri2
            BRICHOS domain
  JOURNAL   Mol Cell Neurosci 125, 103843 (2023)
   PUBMED   36935047
  REMARK    GeneRIF: Identification of cytoskeletal proteins as binding
            partners of Bri2 BRICHOS domain.
REFERENCE   5  (bases 1 to 10089)
  AUTHORS   Rhyu,J.M., Park,J., Shin,B.S., Kim,Y.E., Kim,E.J., Kim,K.W. and
            Cho,Y.G.
  TITLE     A Novel c.800G>C Variant of the ITM2B Gene in Familial Korean
            Dementia
  JOURNAL   J Alzheimers Dis 93 (2), 403-409 (2023)
   PUBMED   37038821
  REMARK    GeneRIF: A Novel c.800G>C Variant of the ITM2B Gene in Familial
            Korean Dementia.
REFERENCE   6  (bases 1 to 10089)
  AUTHORS   Vidal,R., Revesz,T., Rostagno,A., Kim,E., Holton,J.L., Bek,T.,
            Bojsen-Moller,M., Braendgaard,H., Plant,G., Ghiso,J. and
            Frangione,B.
  TITLE     A decamer duplication in the 3' region of the BRI gene originates
            an amyloid peptide that is associated with dementia in a Danish
            kindred
  JOURNAL   Proc Natl Acad Sci U S A 97 (9), 4920-4925 (2000)
   PUBMED   10781099
REFERENCE   7  (bases 1 to 10089)
  AUTHORS   Kim,S.H., Wang,R., Gordon,D.J., Bass,J., Steiner,D.F., Lynn,D.G.,
            Thinakaran,G., Meredith,S.C. and Sisodia,S.S.
  TITLE     Furin mediates enhanced production of fibrillogenic ABri peptides
            in familial British dementia
  JOURNAL   Nat Neurosci 2 (11), 984-988 (1999)
   PUBMED   10526337
REFERENCE   8  (bases 1 to 10089)
  AUTHORS   Vidal,R., Frangione,B., Rostagno,A., Mead,S., Revesz,T., Plant,G.
            and Ghiso,J.
  TITLE     A stop-codon mutation in the BRI gene associated with familial
            British dementia
  JOURNAL   Nature 399 (6738), 776-781 (1999)
   PUBMED   10391242
REFERENCE   9  (bases 1 to 10089)
  AUTHORS   Pittois,K., Deleersnijder,W. and Merregaert,J.
  TITLE     cDNA sequence analysis, chromosomal assignment and expression
            pattern of the gene coding for integral membrane protein 2B
  JOURNAL   Gene 217 (1-2), 141-149 (1998)
   PUBMED   9795190
REFERENCE   10 (bases 1 to 10089)
  AUTHORS   Ernst,B., Dalby,M.A. and Dalby,A.
  TITLE     Luria testing in demented patients
  JOURNAL   Acta Neurol Scand 46 (S43), 97-98 (1970)
   PUBMED   5466275
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA087198.1, AK130048.1 and
            AL139322.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 14, 2018 this sequence version replaced NM_021999.4.
            
            Summary: Amyloid precursor proteins are processed by beta-secretase
            and gamma-secretase to produce beta-amyloid peptides which form the
            characteristic plaques of Alzheimer disease. This gene encodes a
            transmembrane protein which is processed at the C-terminus by furin
            or furin-like proteases to produce a small secreted peptide which
            inhibits the deposition of beta-amyloid. Mutations which result in
            extension of the C-terminal end of the encoded protein, thereby
            increasing the size of the secreted peptide, are associated with
            two neurogenerative diseases, familial British dementia and
            familial Danish dementia. [provided by RefSeq, Oct 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK130048.1, AK000503.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000647800.2/ ENSP00000497221.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-386               DA087198.1         69-454
            387-1821            AK130048.1         391-1825
            1822-10089          AL139322.13        94321-102588
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.2"
     gene            1..10089
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="integral membrane protein 2B"
                     /db_xref="GeneID:9445"
                     /db_xref="HGNC:HGNC:6174"
                     /db_xref="MIM:603904"
     exon            1..272
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..956
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="BRICHOS domain containing 2B; transmembrane protein
                     BRI; ABri/ADan amyloid peptide; immature BRI2; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="integral membrane protein 2B"
                     /protein_id="NP_068839.1"
                     /db_xref="CCDS:CCDS9409.1"
                     /db_xref="GeneID:9445"
                     /db_xref="HGNC:HGNC:6174"
                     /db_xref="MIM:603904"
                     /translation="MVKVTFNSALAQKEAKKDEPKSGEEALIIPPDAVAVDCKDPDDV
                     VPVGQRRAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGIKYIKDDVILNE
                     PSADAPAALYQTIEENIKIFEEEEVEFISVPVPEFADSDPANIVHDFNKKLTAYLDLN
                     LDKCYVIPLNTSIVMPPRNLLELLINIKAGTYLPQSYLIHEHMVITDRIENIDHLGFF
                     IYRLCHDKETYKLQRRETIKGIQKREASNCFAIRHFENKFAVETLICS"
     exon            273..401
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     exon            402..608
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     exon            609..719
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     exon            720..870
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     exon            871..10089
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1100..1105
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="hexamer: ATTAAA"
     polyA_site      1120
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="major polyA site"
     regulatory      1579..1584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="hexamer: TATAAA"
     polyA_site      1605
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="major polyA site"
     regulatory      1757..1762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="hexamer: AATAAG"
     polyA_site      1779
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
     regulatory      1800..1805
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="hexamer: ATTAAA"
     polyA_site      1823
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
     regulatory      10066..10071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
                     /note="hexamer: AATAAA"
     polyA_site      10089
                     /gene="ITM2B"
                     /gene_synonym="ABRI; BRI; BRI2; BRICD2B; E25B; E3-16; FBD;
                     imBRI2; RDGCA"
ORIGIN      
        1 agtagccgcc tctgccgccg cggagcttcc cgaacctctt cagccgcccg gagccgctcc
       61 cggagcccgg ccgtagaggc tgcaatcgca gccgggagcc cgcagcccgc gccccgagcc
      121 cgccgccgcc cttcgagggc gccccaggcc gcgccatggt gaaggtgacg ttcaactccg
      181 ctctggccca gaaggaggcc aagaaggacg agcccaagag cggcgaggag gcgctcatca
      241 tcccccccga cgccgtcgcg gtggactgca aggacccaga tgatgtggta ccagttggcc
      301 aaagaagagc ctggtgttgg tgcatgtgct ttggactagc atttatgctt gcaggtgtta
      361 ttctaggagg agcatacttg tacaaatatt ttgcacttca accagatgac gtgtactact
      421 gtggaataaa gtacatcaaa gatgatgtca tcttaaatga gccctctgca gatgccccag
      481 ctgctctcta ccagacaatt gaagaaaata ttaaaatctt tgaagaagaa gaagttgaat
      541 ttatcagtgt gcctgtccca gagtttgcag atagtgatcc tgccaacatt gttcatgact
      601 ttaacaagaa acttacagcc tatttagatc ttaacctgga taagtgctat gtgatccctc
      661 tgaacacttc cattgttatg ccacccagaa acctactgga gttacttatt aacatcaagg
      721 ctggaaccta tttgcctcag tcctatctga ttcatgagca catggttatt actgatcgca
      781 ttgaaaacat tgatcacctg ggtttcttta tttatcgact gtgtcatgac aaggaaactt
      841 acaaactgca acgcagagaa actattaaag gtattcagaa acgtgaagcc agcaattgtt
      901 tcgcaattcg gcattttgaa aacaaatttg ccgtggaaac tttaatttgt tcttgaacag
      961 tcaagaaaaa cattattgag gaaaattaat atcacagcat aaccccaccc tttacatttt
     1021 gtgcagtgat tattttttaa agtcttcttt catgtaagta gcaaacaggg ctttactatc
     1081 ttttcatctc attaattcaa ttaaaaccat taccttaaaa tttttttctt tcgaagtgtg
     1141 gtgtctttta tatttgaatt agtaactgta tgaagtcata gataatagta catgtcacct
     1201 taggtagtag gaagaattac aatttcttta aatcatttat ctggattttt atgttttatt
     1261 agcattttca agaagacgga ttatctagag aataatcata tatatgcata cgtaaaaatg
     1321 gaccacagtg acttatttgt agttgttagt tgccctgcta cctagtttgt tagtgcattt
     1381 gagcacacat tttaattttc ctctaattaa aatgtgcagt attttcagtg tcaaatatat
     1441 ttaactattt agagaatgat ttccaccttt atgttttaat atcctaggca tctgctgtaa
     1501 taatatttta gaaaatgttt ggaatttaag aaataacttg tgttactaat ttgtataacc
     1561 catatctgtg caatggaata taaatatcac aaagttgttt aactagactg cgtgttgttt
     1621 ttcccgtata ataaaaccaa agaatagttt ggttcttcaa atcttaagag aatccacata
     1681 aaagaagaaa ctatttttta aaaattcact tctatatata caatgagtaa aatcacagat
     1741 tttttcttta aataaaaata agtcatttta ataactaaac cagattcttt gtggatacta
     1801 ttaaagtaac atttaagcct caaccttgaa tgatgttgtt taatataaaa tgcttttatt
     1861 tttgatggaa aggcaatcaa agcctaaagt ttccaaggaa cgtaaaataa ctgtgcttct
     1921 ctgagaatgc ccgcctcaca agtggaacat tttgagttaa taatgatgat ttttatgttt
     1981 ctaaggttgc ttcctctctg aaataattca gaatccaccc ccatttaata aaatacctat
     2041 gattcatttc acctcaagtt cctaagacaa aaaatttcaa ttctgacttt aggaggtcag
     2101 agtttttaaa gcttccaact cttaaaggat ttagtctaga aaccaattct aattcaaaag
     2161 gatattattt actcatggct tttcatttca tttcagtaat attgtcagaa ctttaagtca
     2221 gtgagttggg tattcaggaa ttaacaattt tagaagacaa tatgtttcaa taaaatgaga
     2281 attggttttt cctactttcc aggctctaaa attacatgaa ttttcaggga gctgtgaaca
     2341 tttaggagca cattattcat tcagcatctt ttatatgccc aacatatgat attgtgttga
     2401 agatatatct gcaaagaaga aaaaaagcca ctgccgtcgt ggagactcac aatctaggaa
     2461 aagagaggga cagcaaacaa ctcattacaa tacattggga tcagtagtgt gagggagaaa
     2521 aacaggtcta ggttctttgg aaaacattta ggagagtcaa tgagactttc agaatgtgaa
     2581 gtgcatggga ttaggtcatg gaagaagacc atgtatgtca tcttgaggac ttcattctgt
     2641 agaccatggg ccactattga agttttgcat tttggaagga gcttattagt ttgttttcct
     2701 gttgattata gtagtaatgc agtatcaggg tagctgaact gcaataataa tcccaacatc
     2761 tcactggctt catgcagtag gaatatgctt tcatcacagt ccagtatggc aggcaacctc
     2821 cagactccca gggacccaaa cttacacttt actaaatgta attctttcat ttgaattttt
     2881 aaaggttttt gtataatcat cttagtaaaa catttgataa ttcttcacaa catttgcaat
     2941 cattgatttg gccagattgg tctaacatgg tacccatgat tttacttcat ttccagtact
     3001 attctgataa tttcctttag agtcaattga gtagagagta cccctctcac ctaagcttag
     3061 tcatgtccca ggaattttgg aactccaact gagatttcag gctcattatt cattctaggg
     3121 ctgaatgaat aatgtgccct ttgaaccctt gtgccatgct ggaaactgtg gtcttggtgt
     3181 ggcatggaaa aaccagaggg tgagggatgt ggatgagaag atgacagaga atcagacatg
     3241 cagagaggac tgccaggtct cccagtgggt tccagggaga ctcagctatc cttccctctt
     3301 cttgagttct gtcttagtcc attcgggcta ctataacaaa ataccataga ctgagtggct
     3361 ttaaacaaca gaagcttgtt tctcacagtt ccagaagctg gaaagttcaa gatcaagtca
     3421 cctgcagatt cagtgtctga tgaggtcctc cttcctcaca gaaagctctc ttctctctgt
     3481 gaccttatat cacagatgac tctgggttcc tcttacaagg acactagttc cattcatggg
     3541 ggttccactc tcatgaccta acacctctca aaggccccat ttcctaatac tgtcaccttg
     3601 ggggtaagga tttcaacatg aattctgggg ggacacaaac attcaaacca tagcaagttc
     3661 cataatgcac cccatcatat gtgtgtgtgt gtgtatttat ttatttatct aagatagctc
     3721 tagtttgctg ctttgcaaca cattctgccc ctattgcatt ggtttaaaaa aaaagtacag
     3781 aagtcaagat gagactatat aaatcttgac aagttgttgg ccgtgattgc tcttttgtga
     3841 tgcaggagtg agaatctgga gaatctggct taagaggtgg ctgagaatta aaataatagg
     3901 catgaaaatg cttcatacag ctctgtacag ttgttgacag tgcctttttt gaaacctatc
     3961 actgctatat gttagcaagc aaacactgat cttgttttgt aatgtgtaat tgttcacttc
     4021 tgctttccct gtatcccttt ttagattcca cacccatatt agtgagaagc aagaaaaaaa
     4081 aatgtacaca gtgtcctaaa tgttgtttat ctcgaataat agaagtagga gtgtaaaact
     4141 taaaggctga ttaataatgc aaatgaaaag gcaaatggat tattgaatag aaaaggtcta
     4201 gattctatgt ttgaatgtgg ttatgaatta atatgggcag aatctctaag attctgctaa
     4261 gtgaacattt agtagtttac tagacatctt ctcttccacc tagcaaagag tttgttgccc
     4321 agagttatat aaatcctact ctattgcaca ctaatctttg catgtttaac tctttgggac
     4381 ttaggttcct agataaggac ttcatgtgta tctatttaga aaaagaacat ttcttcattt
     4441 ccacaaagct acacagccat cagttcgttc aatatcttgt ccaaagttat tgctagcgtt
     4501 cttctttctg agtctttgtg gctaatgctt ttttgaggaa gaagcatgat ttagtctcag
     4561 ctctctattg atcattttag gcttttatct tactgaaaaa attaagcaca atttaagatt
     4621 ttttttgaaa tatttactga attttgaggc cataaattat ttcctgatag cacacatgta
     4681 tgggttgtag attccacact tttaatcttc cagccaccta ttgcagattg gttctgagaa
     4741 gcaggatgag tgggagtggg cttaggggtc aaaattgaga cctaagtggt ataaaacaag
     4801 ataatcctag cttcctccca cttaaaataa tttgatggga cttctgtatc tgatttttaa
     4861 gcttgcattt gtgtttatat tttcattgta aaaatatcaa ataatacagt ttttgaaggt
     4921 aaaaagtaaa tgttcccgcc cttctaccat cctccttcct cagtcccatt ccccaacagt
     4981 ttaacgtgcg atacaataat gtgtgacaca aaatgaccta gttcttgccc tagtgactaa
     5041 gcagatgcaa acgccttctt ctttccaaac agtgtggtct tatccccgtc gcaaatggag
     5101 gatatttaag aaacccaagc cagaaacccc acagtcttca cagtcactgt gtcctccacc
     5161 tcctagcagc ttcctgctgt tctgctctag tggttctctc tgcttcccca cacccacccg
     5221 taagccacaa caatctttcc aaaacacgaa cttgatggtt tcattgtcac accccagctt
     5281 aaaacacttt cctggcttcc cctggttctt aggataaaga ccaaaatact taatgctgca
     5341 cagtccagtc ccaacccact ctccagcttc atcttaatca cacccctctt tgctcctagt
     5401 gtcctaccac attgcctgac ttactgtttc ttggtaaatg ctattcttac ttcctgaaga
     5461 ttatttccat ctcctcaccc gacacccttc agttagcttc cactcaacct tcagaattta
     5521 gttcaggtga cccttcctca agagaagatt ttcctgaacc attaggaaag gcttctcttg
     5581 tttctcagtc tcatagcatc atctgtctct tttttgtgac ttgaatataa ttaatatttt
     5641 atattttgtc cttgttgctt gtttggttaa tagatttcca ccactagact gctcaatacg
     5701 ttagtagggc acctgacaca tagttgcagc tcttatttat tgaatgaatg aatgattcct
     5761 tcccgtacca ccctagtctt gcccactgtc atttttattc tgggtattat atttcccaac
     5821 cagttgtctg actttctcct cagatctcat ccccatgggt ctgagtgagt tgccttgaat
     5881 ataagattca gtgatggtgt cctgctgcac ttaagagaaa atcctttttt caagccctaa
     5941 aatcctgccc tcactagact tctctcacca tttctctgtt tgcatcctag ccttttagtt
     6001 ttggcctcat ataagcttgc agccctgcct gaaacagttg tctctgtccc cactctaccc
     6061 cctgccctcc acaaagaaaa gttaactctt actcatcttt cagatctcag actaaaagtt
     6121 tcctcagaga tgccaccata gctctctatt tctcatacct ttcatgatac ttcatcacct
     6181 gtcttcatga agtcctcctc tagactatta gcaaaatgct catactgtgt cctcagtatt
     6241 tggcacataa taggttttct tgacactgaa ggaaggaata aacatctggt aactatagct
     6301 agaaaaaaag gaaatacggt atctacttca ctaaaaccaa agggggtttt tttgggcaga
     6361 caatcttcat tattcagatg gaaattaatg tttgttgaat gaatgagcta catttcatgg
     6421 attaactcca tgaaatggag ttcagctagt aagggttata ctttgaggct atttcatctt
     6481 gatgggcttg ttgttcaaag ttagagcctc attttaagta aaatgttctg tgggggccat
     6541 attaagattt tcatatgaca gaagtgtata tatatccaca aatgtttcaa gccaagatta
     6601 tgtcttcttc cataatgcca ccttcttcta ggatagtatt tttgggccag atggttttcc
     6661 acatttaaaa cataccaaca ttcttttaag gcccagtgat tatcaaaagg gattggcact
     6721 acattttaaa atgtaggggg gatttttgat tgtcacagga ctagaggatg ccatttgtat
     6781 ttagtagatg aggccaggca ggatgctaaa tgtcttaaaa gtccgggagt cctgcacaaa
     6841 aaattactgc ccttcttgat ttgccaatta tgcccctgct gggaagcatg aaaggagaga
     6901 gaagagcttt cagaatgtca gggataaata tatccattaa gccttcagac taagatgcag
     6961 gaatagtttt ccatttgggt atgaatacgg caagttctta gaacatttct taaaacataa
     7021 ttagccaaat actagtgtaa cagctacaac ttatctagtt ttaatcaact ccagggaaca
     7081 ttacacttta tttaacccct aattatctga taacccaagt tctgtcagaa acactgctga
     7141 ggcatccagg aagatgagcc tgtgaaattt gagaatcctt catgtctctg gaatgagtcc
     7201 ctagattcta gccctgcagc tagctgacac tctaaaaaat tatgagtgcc ttcctttagc
     7261 tccccataga aatgtctttg tataaccatg gcatgatatt attctcttga cttttgttga
     7321 ggattgatag aaataaaaat gagccagaaa tgtttcttca gcttttgtaa agtgctgtta
     7381 aaagcattat gtcatatcta gtacttctta tctaagtatt ttaaaattta gtgttaggaa
     7441 aatttttttc acataggata catgttaata ttaggtagta catctaaaga attcatttgc
     7501 ttcccttttg ccttggttgc tgggaaactc agagccaaac tcggtgctta gattaattac
     7561 tttcagccta ggtttttctg tatatcaccc tttcggtagg gcttgaagta acccttcagt
     7621 ttgctcatat tttgttgtga gttttataga tagtttacat gcaacgaaat gcatatatct
     7681 taagtgttca actcagtgag ttttgacact tttctttgtc catgttaaca atcatcaaaa
     7741 cattttcatt gccccagaaa ataccattgt gctcctttcc ggcaaatatt ccttccctct
     7801 gctaaaacta ctttttcatt tctatgacca tagttcagtt tttggctatt cttgaacttt
     7861 atttgaatgg aatcgtacat atataatctt tttgtttggc ttttaaaact cagtataacg
     7921 ttttgacatt tgtccatgtt gttgcatgac tcagcagttc tttcttttca gttgctgtgt
     7981 agtagtccat ttcttttggt tttgtgtgtg tgtgtgtgct ttttaaattt attttttatt
     8041 tttttttttt tgagacagag tctggctctg tcaactcagg ctggagtgca gtggcatcat
     8101 ctcggctcac tgcaatttct gtctcccagg ctcaagccat cctctaacct cagcctcccg
     8161 agtagctaag actctacagg cgtatgccac catacctagt ttgtttttgt tatttttttt
     8221 agtacagatg gggttttgcc atgttgtcca ggctggtctc gaactcctgg gctcaaagca
     8281 atccaccctc ctcggcctcc caaagtgcta ggattacaag tgtgaggcac tgtgccctgc
     8341 cagtattcca ttttttgtgt ataccacaat ttatcatttt tttctgtgta ggtattttca
     8401 ttgtttccaa gttgggaagt atgaataaag ttgctatgaa catgcatgta caagtctttg
     8461 tagatagctc atgttttaaa aagtgtttgt ttaatgattt cattgcaaaa tcatctttat
     8521 tgtaaacata tctttttgag ggtagattgg taaaatatac ataataaata catgacaatg
     8581 caggagtcca agtattatta ggtttacagt cataacaaac accaagaacc atttataaat
     8641 ttcagtttct cattgtttta gaatggaagg tctaaccaaa tatgtaatgt atcccttcaa
     8701 gtaaactagt ataacttggg taccttctca tataaattcc ccttaaggca tacgttgctg
     8761 tcttttccaa attcccattt tgcttgtcca ataggcatta tcagatacaa catgttcaaa
     8821 ggcaaacttg attttcctcc catggtttcc tcatctcagc tccttttttt ttccagttgc
     8881 acaggctaaa aataggaagt cattctagat tcctctcttt ttcataaacc tcacatcaaa
     8941 tccatcagcc aatctggtgt gctctacctt caagatacgg attatccaga attcagccgc
     9001 ttctcactgt ctctactgct aacatagtag tcaaagctgc tatcatctct tgcttggatt
     9061 ggtgcaatgg cctcacaact gatcttcctg cttccatact tgacccctag agcctcttct
     9121 aaacagagta gctagagcga tcttttctaa atctaagcca catcatgtca ctcccctgct
     9181 caaatcccaa cattttattc agaataaaag tcgaacaatg ccctacctgt aggtaatcta
     9241 ggcctccaaa gctcttctta catcacctcc tgtaactctc cccctttctc actcctctac
     9301 agccacacag ctgcctcact ttcctttgaa cgtaccaagg gcaatcctgc ttcagagcct
     9361 ttgcacttgc tgtttctggg ccaggaattc cacaccctcc ttccctcaca tgtatcccaa
     9421 ggctcactgc cttccttcag ttaagtctgc tggaatgtca cttcattagg tattgcctga
     9481 tcaccttttc taaagtgagt ctcacctcca ccctcaatgc tggcaatgtc tgtctgtagt
     9541 gtattcttaa caatacagat tagcacaatg caggccagac catgaagact gtttcaagtt
     9601 cgaggatggg caaaaataat gactaggact tggctcaaga ttcctcaaac agccaataaa
     9661 caatgcaaag aataaatgag agagtaattg gagacaagct ttcccttggc ttcccttctt
     9721 ttctcacaga ggcttttcca gaaggtggtc atttccatag cccatgtgga atgagatgtc
     9781 cactagggca gagaagcaca gaaccgtggg cactgcttcc cttcctttaa ccctatcata
     9841 ttgctgccca gtgagcaata tggtcattgg tcaagaaggc ttatgcccag gtatccctgt
     9901 ggctatgagc attgctcctt atctatgcct ctgctcttag acttttccat tctgccctct
     9961 taggggcctt cctggccatg accacactac cttatttttc tctatagtac ttatcaccat
    10021 ctgacatgta tttatgtttg tatttgttta ttgtctgtct ctacaaataa aatgtaaatt
    10081 ccctaaaaa
//
