LOCUS       NP_000623               1152 aa            linear   PRI 27-APR-2025
DEFINITION  integrin alpha-M isoform 2 precursor [Homo sapiens].
ACCESSION   NP_000623
VERSION     NP_000623.2
DBSOURCE    REFSEQ: accession NM_000632.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1152)
  AUTHORS   Liu,Y.G., Peng,H.R., Ren,R.W., Zhao,P. and Zhao,L.J.
  TITLE     CD11b maintains West Nile virus replication through modulation of
            immune response in human neuroblastoma cells
  JOURNAL   Virol J 21 (1), 158 (2024)
   PUBMED   39004752
  REMARK    GeneRIF: CD11b maintains West Nile virus replication through
            modulation of immune response in human neuroblastoma cells.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1152)
  AUTHORS   Yang,C., Li,L., Ye,Z., Zhang,A., Bao,Y., Wu,X., Ren,G., Jiang,C.,
            Wang,O. and Wang,Z.
  TITLE     Mechanisms underlying neutrophils adhesion to triple-negative
            breast cancer cells via CD11b-ICAM1 in promoting breast cancer
            progression
  JOURNAL   Cell Commun Signal 22 (1), 340 (2024)
   PUBMED   38907234
  REMARK    GeneRIF: Mechanisms underlying neutrophils adhesion to
            triple-negative breast cancer cells via CD11b-ICAM1 in promoting
            breast cancer progression.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1152)
  AUTHORS   Duan,M., Zhang,X., Lou,Y., Feng,J., Guo,P., Ye,S., Lv,P. and
            Chen,Y.
  TITLE     Deletion of Tmem268 in mice suppresses anti-infectious immune
            responses by downregulating CD11b signaling
  JOURNAL   EMBO Rep 25 (6), 2550-2570 (2024)
   PUBMED   38730209
REFERENCE   4  (residues 1 to 1152)
  AUTHORS   Hou,L., Li,Z., Guo,X., Lv,J., Chong,Z., Xiao,Y., Zhang,L. and Li,Z.
  TITLE     ITGAM is a critical gene in ischemic stroke
  JOURNAL   Aging (Albany NY) 16 (8), 6852-6867 (2024)
   PUBMED   38637126
  REMARK    GeneRIF: ITGAM is a critical gene in ischemic stroke.
REFERENCE   5  (residues 1 to 1152)
  AUTHORS   Lou,Y., Li,P.H., Liu,X.Q., Wang,T.X., Liu,Y.L., Chen,C.C. and
            Ma,K.L.
  TITLE     ITGAM-mediated macrophages contribute to basement membrane damage
            in diabetic nephropathy and atherosclerosis
  JOURNAL   BMC Nephrol 25 (1), 72 (2024)
   PUBMED   38413872
  REMARK    GeneRIF: ITGAM-mediated macrophages contribute to basement membrane
            damage in diabetic nephropathy and atherosclerosis.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1152)
  AUTHORS   Pahl,H.L., Rosmarin,A.G. and Tenen,D.G.
  TITLE     Characterization of the myeloid-specific CD11b promoter
  JOURNAL   Blood 79 (4), 865-870 (1992)
   PUBMED   1346576
REFERENCE   7  (residues 1 to 1152)
  AUTHORS   Shelley,C.S. and Arnaout,M.A.
  TITLE     The promoter of the CD11b gene directs myeloid-specific and
            developmentally regulated expression
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10525-10529 (1991)
   PUBMED   1683702
REFERENCE   8  (residues 1 to 1152)
  AUTHORS   Hickstein,D.D., Hickey,M.J., Ozols,J., Baker,D.M., Back,A.L. and
            Roth,G.J.
  TITLE     cDNA sequence for the alpha M subunit of the human neutrophil
            adherence receptor indicates homology to integrin alpha subunits
  JOURNAL   Proc Natl Acad Sci U S A 86 (1), 257-261 (1989)
   PUBMED   2563162
REFERENCE   9  (residues 1 to 1152)
  AUTHORS   Corbi,A.L., Kishimoto,T.K., Miller,L.J. and Springer,T.A.
  TITLE     The human leukocyte adhesion glycoprotein Mac-1 (complement
            receptor type 3, CD11b) alpha subunit. Cloning, primary structure,
            and relation to the integrins, von Willebrand factor and factor B
  JOURNAL   J Biol Chem 263 (25), 12403-12411 (1988)
   PUBMED   2457584
REFERENCE   10 (residues 1 to 1152)
  AUTHORS   Arnaout,M.A., Gupta,S.K., Pierce,M.W. and Tenen,D.G.
  TITLE     Amino acid sequence of the alpha subunit of human leukocyte
            adhesion receptor Mo1 (complement receptor type 3)
  JOURNAL   J Cell Biol 106 (6), 2153-2158 (1988)
   PUBMED   2454931
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK057856.1, BC096348.3,
            AA436312.1 and AC093520.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 20, 2006 this sequence version replaced NP_000623.1.
            
            Summary: This gene encodes the integrin alpha M chain. Integrins
            are heterodimeric integral membrane proteins composed of an alpha
            chain and a beta chain. This I-domain containing alpha integrin
            combines with the beta 2 chain (ITGB2) to form a leukocyte-specific
            integrin referred to as macrophage receptor 1 ('Mac-1'), or
            inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2
            integrin is important in the adherence of neutrophils and monocytes
            to stimulated endothelium, and also in the phagocytosis of
            complement coated particles. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Mar 2009].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1.
            The resulting isoform (2) lacks one internal amino acid, compared
            to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.90372.1, BC096346.3
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000544665.9/ ENSP00000441691.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     Protein         1..1152
                     /product="integrin alpha-M isoform 2 precursor"
                     /note="antigen CD11b (p170); macrophage antigen alpha
                     polypeptide; neutrophil adherence receptor alpha-M
                     subunit; integrin alpha-M; CR-3 alpha chain; leukocyte
                     adhesion receptor MO1; CD11 antigen-like family member B;
                     cell surface glycoprotein MAC-1 subunit alpha; integrin,
                     alpha M (complement component 3 receptor 3 subunit);
                     macrophage-1 antigen alpha subunit"
                     /calculated_mol_wt=125472
     sig_peptide     1..16
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=1725
     mat_peptide     17..1152
                     /product="Integrin alpha-M. /id=PRO_0000016289"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
                     /calculated_mol_wt=125472
     Region          18..75
                     /region_name="FG-GAP 1.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          76..135
                     /region_name="FG-GAP 2.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Site            86
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Region          149..324
                     /region_name="vWA_integrins_alpha_subunit"
                     /note="Integrins are a class of adhesion receptors that
                     link the extracellular matrix to the cytoskeleton and
                     cooperate with growth factor receptors to promote celll
                     survival, cell cycle progression and cell migration.
                     Integrins consist of an alpha and a beta...; cd01469"
                     /db_xref="CDD:238746"
     Site            order(149,151,172,252,254,283,313)
                     /site_type="active"
                     /note="integrin inhibitor binding pocket [active]"
                     /db_xref="CDD:238746"
     Site            order(156,158,160,225,258)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238746"
     Site            order(158..160,162,225)
                     /site_type="active"
                     /note="integrin-collagen binding site [active]"
                     /db_xref="CDD:238746"
     Site            240
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Region          339..390
                     /region_name="FG-GAP 3.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          391..442
                     /region_name="FG-GAP 4.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Site            391
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Region          443..503
                     /region_name="FG-GAP 5.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          453..509
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Site            469
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Region          506..564
                     /region_name="FG-GAP 6.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          516..562
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          569..629
                     /region_name="FG-GAP 7.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          615..978
                     /region_name="Integrin_alpha2"
                     /note="Integrin alpha; pfam08441"
                     /db_xref="CDD:462478"
     Site            692
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            696
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            734
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            801
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            880
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            900
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            911
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            940
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            946
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            978
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            993
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            1021
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            1044
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            1050
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            1075
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P11215.2)"
     Site            1105..1128
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     Region          1129..1143
                     /region_name="Integrin_alpha"
                     /note="Integrin alpha cytoplasmic region; pfam00357"
                     /db_xref="CDD:459778"
     Region          1131..1135
                     /region_name="GFFKR motif"
                     /note="propagated from UniProtKB/Swiss-Prot (P11215.2)"
     CDS             1..1152
                     /gene="ITGAM"
                     /gene_synonym="CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6"
                     /coded_by="NM_000632.4:91..3549"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /db_xref="CCDS:CCDS45470.1"
                     /db_xref="GeneID:3684"
                     /db_xref="HGNC:HGNC:6149"
                     /db_xref="MIM:120980"
ORIGIN      
        1 malrvlllta ltlchgfnld tenamtfqen argfgqsvvq lqgsrvvvga pqeivaanqr
       61 gslyqcdyst gscepirlqv pveavnmslg lslaattspp qllacgptvh qtcsentyvk
      121 glcflfgsnl rqqpqkfpea lrgcpqedsd iaflidgsgs iiphdfrrmk efvstvmeql
      181 kksktlfslm qyseefrihf tfkefqnnpn prslvkpitq llgrthtatg irkvvrelfn
      241 itngarknaf kilvvitdge kfgdplgyed vipeadregv iryvigvgda frseksrqel
      301 ntiaskpprd hvfqvnnfea lktiqnqlre kifaiegtqt gssssfehem sqegfsaait
      361 sngpllstvg sydwaggvfl ytskekstfi nmtrvdsdmn daylgyaaai ilrnrvqslv
      421 lgapryqhig lvamfrqntg mwesnanvkg tqigayfgas lcsvdvdsng stdlvligap
      481 hyyeqtrggq vsvcplprgr arwqcdavly geqgqpwgrf gaaltvlgdv ngdkltdvai
      541 gapgeednrg avylfhgtsg sgispshsqr iagsklsprl qyfgqslsgg qdltmdglvd
      601 ltvgaqghvl llrsqpvlrv kaimefnpre varnvfecnd qvvkgkeage vrvclhvqks
      661 trdrlregqi qsvvtydlal dsgrphsrav fnetknstrr qtqvlgltqt cetlklqlpn
      721 ciedpvspiv lrlnfslvgt plsafgnlrp vlaedaqrlf talfpfeknc gndnicqddl
      781 sitfsfmsld clvvggpref nvtvtvrndg edsyrtqvtf ffpldlsyrk vstlqnqrsq
      841 rswrlacesa sstevsgalk stscsinhpi fpensevtfn itfdvdskas lgnklllkan
      901 vtsennmprt nktefqlelp vkyavymvvt shgvstkyln ftasentsrv mqhqyqvsnl
      961 gqrslpislv flvpvrlnqt viwdrpqvtf senlsstcht kerlpshsdf laelrkapvv
     1021 ncsiavcqri qcdipffgiq eefnatlkgn lsfdwyikts hnhllivsta eilfndsvft
     1081 llpgqgafvr sqtetkvepf evpnplpliv gssvggllll alitaalykl gffkrqykdm
     1141 mseggppgae pq
//
