LOCUS       NP_002200               1170 aa            linear   PRI 27-APR-2025
DEFINITION  integrin alpha-L isoform a precursor [Homo sapiens].
ACCESSION   NP_002200
VERSION     NP_002200.2
DBSOURCE    REFSEQ: accession NM_002209.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1170)
  AUTHORS   Zhang,R., Zhu,G., Li,Z., Meng,Z., Huang,H., Ding,C., Wang,Y.,
            Chen,C., Li,Y., Liu,H. and Chen,J.
  TITLE     ITGAL expression in non-small-cell lung cancer tissue and its
            association with immune infiltrates
  JOURNAL   Front Immunol 15, 1382231 (2024)
   PUBMED   38646528
  REMARK    GeneRIF: ITGAL expression in non-small-cell lung cancer tissue and
            its association with immune infiltrates.
            Erratum:[Front Immunol. 2024 Apr 26;15:1415814. doi:
            10.3389/fimmu.2024.1415814. PMID: 38736883]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1170)
  AUTHORS   Lacouture,C., Chaves,B., Guipouy,D., Houmadi,R., Duplan-Eche,V.,
            Allart,S., Destainville,N. and Dupre,L.
  TITLE     LFA-1 nanoclusters integrate TCR stimulation strength to tune
            T-cell cytotoxic activity
  JOURNAL   Nat Commun 15 (1), 407 (2024)
   PUBMED   38195629
  REMARK    GeneRIF: LFA-1 nanoclusters integrate TCR stimulation strength to
            tune T-cell cytotoxic activity.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1170)
  AUTHORS   Wang,Q., Xiao,G., Li,N., Jiang,X. and Li,C.
  TITLE     lncRNA PCBP1-AS1 mediated downregulation of ITGAL as a prognostic
            biomarker in lung adenocarcinoma
  JOURNAL   Aging (Albany NY) 15 (10), 4510-4523 (2023)
   PUBMED   37256932
  REMARK    GeneRIF: lncRNA PCBP1-AS1 mediated downregulation of ITGAL as a
            prognostic biomarker in lung adenocarcinoma.
REFERENCE   4  (residues 1 to 1170)
  AUTHORS   Hickman,A., Koetsier,J., Kurtanich,T., Nielsen,M.C., Winn,G.,
            Wang,Y., Bentebibel,S.E., Shi,L., Punt,S., Williams,L.,
            Haymaker,C., Chesson,C.B., Fa'ak,F., Dominguez,A.L., Jones,R.,
            Kuiatse,I., Caivano,A.R., Khounlo,S., Warier,N.D., Marathi,U.,
            Market,R.V., Biediger,R.J., Craft,J.W. Jr., Hwu,P., Davies,M.A.,
            Woodside,D.G., Vanderslice,P., Diab,A., Overwijk,W.W. and
            Hailemichael,Y.
  TITLE     LFA-1 activation enriches tumor-specific T cells in a cold tumor
            model and synergizes with CTLA-4 blockade
  JOURNAL   J Clin Invest 132 (13) (2022)
   PUBMED   35552271
  REMARK    GeneRIF: LFA-1 activation enriches tumor-specific T cells in a cold
            tumor model and synergizes with CTLA-4 blockade.
REFERENCE   5  (residues 1 to 1170)
  AUTHORS   Garcia-Salido,A., Cuenca-Carcelen,S. and Castillo-Robleda,A.
  TITLE     CD64, CD11a and CD18 leukocytes expression in children with
            SARS-CoV-2 multisystem inflammatory syndrome versus children with
            Kawasaki disease: Similar but not the same
  JOURNAL   Med Clin (Barc) 156 (2), 89-91 (2021)
   PUBMED   33160625
  REMARK    GeneRIF: CD64, CD11a and CD18 leukocytes expression in children
            with SARS-CoV-2 multisystem inflammatory syndrome versus children
            with Kawasaki disease: Similar but not the same.
REFERENCE   6  (residues 1 to 1170)
  AUTHORS   Fawcett,J., Holness,C.L., Needham,L.A., Turley,H., Gatter,K.C.,
            Mason,D.Y. and Simmons,D.L.
  TITLE     Molecular cloning of ICAM-3, a third ligand for LFA-1,
            constitutively expressed on resting leukocytes
  JOURNAL   Nature 360 (6403), 481-484 (1992)
   PUBMED   1448173
REFERENCE   7  (residues 1 to 1170)
  AUTHORS   Alvarez,V., Pulido,R., Campanero,M.R., Paraiso,V., de
            Landazuri,M.O. and Sanchez-Madrid,F.
  TITLE     Differentially regulated cell surface expression of leukocyte
            adhesion receptors on neutrophils
  JOURNAL   Kidney Int 40 (5), 899-905 (1991)
   PUBMED   1762294
REFERENCE   8  (residues 1 to 1170)
  AUTHORS   Maurer,D., Holter,W., Majdic,O., Fischer,G.F. and Knapp,W.
  TITLE     CD27 expression by a distinct subpopulation of human B lymphocytes
  JOURNAL   Eur J Immunol 20 (12), 2679-2684 (1990)
   PUBMED   1702722
REFERENCE   9  (residues 1 to 1170)
  AUTHORS   Dustin,M.L. and Springer,T.A.
  TITLE     T-cell receptor cross-linking transiently stimulates adhesiveness
            through LFA-1
  JOURNAL   Nature 341 (6243), 619-624 (1989)
   PUBMED   2477710
REFERENCE   10 (residues 1 to 1170)
  AUTHORS   Larson,R.S., Corbi,A.L., Berman,L. and Springer,T.
  TITLE     Primary structure of the leukocyte function-associated molecule-1
            alpha subunit: an integrin with an embedded domain defining a
            protein superfamily
  JOURNAL   J Cell Biol 108 (2), 703-712 (1989)
   PUBMED   2537322
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314246.1, BC008777.2 and
            CA430398.1.
            
            On Feb 8, 2008 this sequence version replaced NP_002200.1.
            
            Summary: ITGAL encodes the integrin alpha L chain. Integrins are
            heterodimeric integral membrane proteins composed of an alpha chain
            and a beta chain. This I-domain containing alpha integrin combines
            with the beta 2 chain (ITGB2) to form the integrin lymphocyte
            function-associated antigen-1 (LFA-1), which is expressed on all
            leukocytes. LFA-1 plays a central role in leukocyte intercellular
            adhesion through interactions with its ligands, ICAMs 1-3
            (intercellular adhesion molecules 1 through 3), and also functions
            in lymphocyte costimulatory signaling. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y00796.1, AK314246.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356798.11/ ENSP00000349252.5
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     Protein         1..1170
                     /product="integrin alpha-L isoform a precursor"
                     /note="antigen CD11A (p180), lymphocyte
                     function-associated antigen 1, alpha polypeptide; integrin
                     alpha-L; LFA-1 alpha; integrin gene promoter; LFA-1A; CD11
                     antigen-like family member A; leukocyte adhesion
                     glycoprotein LFA-1 alpha chain; leukocyte
                     function-associated molecule 1 alpha chain; integrin,
                     alpha L (antigen CD11A (p180), lymphocyte
                     function-associated antigen 1; alpha polypeptide)"
                     /calculated_mol_wt=126115
     sig_peptide     1..25
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2673
     mat_peptide     26..1170
                     /product="integrin alpha-L isoform a"
                     /calculated_mol_wt=126115
     Region          31..82
                     /region_name="FG-GAP 1.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Site            65
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Region          83..141
                     /region_name="FG-GAP 2.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Site            89
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Region          155..323
                     /region_name="vWA_integrins_alpha_subunit"
                     /note="Integrins are a class of adhesion receptors that
                     link the extracellular matrix to the cytoskeleton and
                     cooperate with growth factor receptors to promote celll
                     survival, cell cycle progression and cell migration.
                     Integrins consist of an alpha and a beta...; cd01469"
                     /db_xref="CDD:238746"
     Site            order(155,157,178,258,260,282,312)
                     /site_type="active"
                     /note="integrin inhibitor binding pocket [active]"
                     /db_xref="CDD:238746"
     Site            order(162,164,166,231,264)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238746"
     Site            order(164..166,168,231)
                     /site_type="active"
                     /note="integrin-collagen binding site [active]"
                     /db_xref="CDD:238746"
     Region          170..349
                     /region_name="I-domain (insertion domain)"
     Site            188
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19349973; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Region          390..445
                     /region_name="FG-GAP 4.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Region          446..506
                     /region_name="FG-GAP 5.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Region          456..503
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          507..563
                     /region_name="FG-GAP 6.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Region          517..569
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          567..627
                     /region_name="FG-GAP 7.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00803"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Region          579..>603
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          613..999
                     /region_name="Integrin_alpha2"
                     /note="Integrin alpha; pfam08441"
                     /db_xref="CDD:462478"
     Site            649
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            670
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            726
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            730
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            862
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            885
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            897
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            1060
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            1071
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     Site            1089..1112
                     /site_type="transmembrane region"
     Region          1113..1127
                     /region_name="Integrin_alpha"
                     /note="Integrin alpha cytoplasmic region; pfam00357"
                     /db_xref="CDD:459778"
     Region          1115..1119
                     /region_name="GFFKR motif"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Region          1128..1170
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P20701.3)"
     Site            1165
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:16301335; propagated from
                     UniProtKB/Swiss-Prot (P20701.3)"
     CDS             1..1170
                     /gene="ITGAL"
                     /gene_synonym="CD11A; LFA-1; LFA1A"
                     /coded_by="NM_002209.3:97..3609"
                     /note="isoform a precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS32433.1"
                     /db_xref="GeneID:3683"
                     /db_xref="HGNC:HGNC:6148"
                     /db_xref="MIM:153370"
ORIGIN      
        1 mkdscitvma mallsgffff apassynldv rgarsfsppr agrhfgyrvl qvgngvivga
       61 pgegnstgsl yqcqsgtghc lpvtlrgsny tskylgmtla tdptdgsila cdpglsrtcd
      121 qntylsglcy lfrqnlqgpm lqgrpgfqec ikgnvdlvfl fdgsmslqpd efqkildfmk
      181 dvmkklsnts yqfaavqfst syktefdfsd yvkrkdpdal lkhvkhmlll tntfgainyv
      241 atevfreelg arpdatkvli iitdgeatds gnidaakdii ryiigigkhf qtkesqetlh
      301 kfaskpasef vkildtfekl kdlftelqkk iyviegtskq dltsfnmels ssgisadlsr
      361 ghavvgavga kdwaggfldl kadlqddtfi gnepltpevr agylgytvtw lpsrqktsll
      421 asgapryqhm grvllfqepq ggghwsqvqt ihgtqigsyf ggelcgvdvd qdgetellli
      481 gaplfygeqr ggrvfiyqrr qlgfeevsel qgdpgyplgr fgeaitaltd ingdglvdva
      541 vgapleeqga vyifngrhgg lspqpsqrie gtqvlsgiqw fgrsihgvkd legdgladva
      601 vgaesqmivl ssrpvvdmvt lmsfspaeip vhevecsyst snkmkegvni ticfqiksli
      661 pqfqgrlvan ltytlqldgh rtrrrglfpg grhelrrnia vttsmsctdf sfhfpvcvqd
      721 lispinvsln fslweeegtp rdqraqgkdi ppilrpslhs etweipfekn cgedkkcean
      781 lrvsfspars ralrltafas lsvelslsnl eedaywvqld lhfppglsfr kvemlkphsq
      841 ipvsceelpe esrllsrals cnvsspifka ghsvalqmmf ntlvnsswgd svelhanvtc
      901 nnedsdlled nsattiipil ypiniliqdq edstlyvsft pkgpkihqvk hmyqvriqps
      961 ihdhniptle avvgvpqpps egpithqwsv qmeppvpchy edlerlpdaa epclpgalfr
     1021 cpvvfrqeil vqvigtlelv geieassmfs lcsslsisfn sskhfhlygs naslaqvvmk
     1081 vdvvyekqml ylyvlsgigg llllllifiv lykvgffkrn lkekmeagrg vpngipaeds
     1141 eqlasgqeag dpgclkplhe kdsesgggkd
//
